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At least 1,657 records · Page 92
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Single-nuclei transcriptome analysis of channel catfish spleen provides insight into the immunome of an aquaculture-relevant species

The catfish industry is the largest sector of U.S. aquaculture production. Given its role in food production, the catfish immune response to industry-relevant pathogens has been extensively studied and has provided crucial information on innate and adaptive immune function during disease progression. To further examine the channel catfish immune system, we performed single-cell RNA sequencing on nuclei isolated from whole spleens, a major lymphoid organ in teleost fish. Libraries were prepared using the 10X Genomics Chromium X with the Next GEM Single Cell 3’ reagents and sequenced on an Illumina sequencer. Each demultiplexed sample was aligned to the Coco_2.0 channel catfish reference assembly, filtered, and counted to generate feature-barcode matrices. From whole spleen samples, outputs were analyzed both individually and as an integrated dataset. The three splenic transcriptome libraries generated an average of 278,717,872 reads from a mean 8,157 cells. The integrated data included 19,613 cells, counts for 20,121 genes, with a median 665 genes/cell. Cluster analysis of all cells identified 17 clusters which were classified as erythroid, hematopoietic stem cells, B cells, T cells, myeloid cells, and endothelial cells. Subcluster analysis was carried out on the immune cell populations. Here, distinct subclusters such as immature B cells, mature B cells, plasma cells, γδ T cells, dendritic cells, and macrophages were further identified. Differential gene expression analyses allowed for the identification of the most highly expressed genes for each cluster and subcluster. This dataset is a rich cellular gene expression resource for investigation of the channel catfish and teleost splenic immunome.

Science & Technology - Other Topics↗
NASA NTRS1967

Foil radiometer accessory improves measurements

The responsiveness of a foil radiometer is increased and its time constant is simultaneously decreased by isolating the foil in a controlled environment. Using an optical system, it is coupled to the media to be measured, and the resulting concentration of energy permits the thermocouple junction temperature to respond quickly.

Schumacher, P. E.↗
NASA NTRS1973

SIRU development. Volume 1: System development

A complete description of the development and initial evaluation of the Strapdown Inertial Reference Unit (SIRU) system is reported. System development documents the system mechanization with the analytic formulation for fault detection and isolation processing structure; the hardware redundancy design and the individual modularity features; the computational structure and facilities; and the initial subsystem evaluation results.

Gilmore, J. P.↗
NASA NTRS1974

Evaluation of coated columbium alloy heat shields for space shuttle thermal protection system application. Volume 3, phase 3: Full size TPS evaluation

The thermal protection system (TPS), designed for incorporation with space shuttle orbiter systems, consists of one primary heat shield thermally and structurally isolated from the test fixture by eight peripheral guard panels, all encompassing an area of approximately 12 sq ft. TPS components include tee-stiffened Cb 752/R-512E heat shields, bi-metallic support posts, panel retainers, and high temperature insulation blankets. The vehicle primary structure was simulated by a titanium skin, frames, and stiffeners. Test procedures, manufacturing processes, and methods of analysis are fully documented. For Vol. 1, see N72-30948; for Vol. 2, see N74-15660.

Baer, J. W.↗
NASA NTRS1977

On interstellar accretion and the rejuvenation of white dwarfs

The paper investigates physical conditions which can give rise to thermonuclear runaways in the hydrogen envelopes of low-luminosity white dwarfs. Specifically, calculations are performed for white dwarfs of 0.5, 0.75, 1, and 1.25 solar masses with envelope masses in the range from 0.00001 to 0.001 solar mass and initial luminosities of 0.00001 to 0.01 times the solar value. It is found that envelope masses as low as 0.0001 solar mass are sufficient to initiate thermonuclear runaways on more massive white dwarfs with luminosities as low as about 0.001 the solar value. The runaway time scales under these conditions, typically of the order of a billion years, are comparable to the time scales for cooling of white dwarfs to these low luminosities. Since time-averaged accretion rates as low as a few times 10 to the -14th power solar mass per year are sufficient to reconstitute such envelopes, also on a time scale of several billion years, it is suggested that the association of novalike events with binary systems may not be unique. Accretion of interstellar matter onto isolated white dwarfs may, under some circumstances, be sufficient to rekindle and perhaps rejuvenate the dwarf. Such evolutionary behavior might define a new and distinct class of objects.

Truran, J. W.↗
NASA NTRS1982

Edge softening of the Shuttle TPS strain isolation pad

Tensile tests and an analytical investigation were performed to characterize the edge softening behavior of the strain isolation pad (SIP) between the Orbiter skin and thermal protection system. The tensile tests were carried out with varying sizes of disk-shaped specimens bonded between aluminum disks. The specimens strength and stiffness were determined on the basis of specimen size, and an analytical model of the microstructural stress-strain characteristics was developed. Strength and stiffness were found to decrease near the free edges because through-the-thickness fibers located there were not anchored. No size dependence at maximum load was observed in specimens between 0.75-4.0 in. thick. In-plane and out-of-plane coupling in deformation was detected. The model gave accurate predictions of the tensile behavior of the SIP as a function of distance to a free edge.

Ransone, P. O.↗
NASA NTRS1983

Measured inplane stability characteristics in hover for an advanced bearingless rotor

A study was made of the inplane stability characteristics of a model of an advanced bearingless main rotor. The four-bladed rotor system was tested for aeroelastic stability in hover for both isolated and body-free conditions. Variations in several hub design parameters were tested, including blade coning and sweep angles, blade inplane structural damping, pitch-link location (delta-free effect), and fuselage structural damping. An analysis of the experimental results shows that parametric stability trends observed from isolated-rotor studies may yield incorrect conclusions regarding coupled rotor-body characteristics. For body-free conditions, the baseline rotor configuration resulted in the best stability margins at 1-g thrust as well as at the nominal design rotor speed. Blade built-in coning had little effect on the body-free damping levels at the inplane/body resonance points. Introducing blade sweep destabilized the rotor at the inplane/body pitch resonance.

Weller, W. H.↗
NASA NTRS1984

The G-002 JUFO-1 payload: Its objectives and results

The JUFO-1 payload basically consists of the mechanical support structure with two shock isolated mounting shelves for the experiment hardware and the service systems. For the envisaged operation time of 72 hours, three 28 VDC batteries, with a total capacity of approx 1,7 kWh are chosen as a partially redundant power source. Data are acquired by a 12 bit PCM system and stored on a tape recorder. Five experiments were conducted: (1) growth of a liquid KH2PO4 salt solution; (2) manufacturing of nickel catalysts; (3) transport mechanisms of heavy metals in water cress shoots; (4) determining the influence of cosmic radiation on four different plant seeds; and (5) use of a microprocessor-sequencer for payload control and sequencing at a low power consumption.

Schmitt, G.↗
NASA NTRS1986

Near-field testing of the 5-meter model of the tetrahedral truss antenna

This report documents the technical results from near-field testing of the General Dynamics 5-meter model of the tetrahedral truss antenna at the Martin Marietta Denver Aerospace facility. A 5-meter square side of the tetrahedral served as the perimeter of the antenna, and a mesh surface and extensive surface contouring cord network was used to create a parabolic aperture shape to within an rms accuracy of 30 mils or better. Pattern measurements were made with offset feed systems radiating at frequencies of 7.73, 11.60, 2.27, and 4.26 (all in GHz). This report discusses the method of collecting the data, system measurement accuracy, the test data compiled, and diagostics and isolation of causes of pattern results. The technique of using near-field phase for measuring surface mechanical tolerances is included. Detailed far field antenna patterns and their implications are provided for all tests conducted.

Kefauver, Neill↗
NASA NTRS1986

Mass and energy balance of the cold Io torus

A new model of the cold Io torus is described. Ions and energy are injected into the system by independent processes so that the mass balance is isolated from the energy balance. The primary source of energy is local ionization and acceleration of hot pickup ions resulting from charge exchange between thermal ions and an extended cloud of Iogenic sulfur and oxygen atoms. The primary energy loss mechanism of the plasma is collisionally excited line emission at optical wavelengths. The primary ion source is radial diffusion inward from the hot torus on a time scale of 140-710 days. The primary ion loss mechanism is a novel two-step enhanced recombination mechanism involving charge exchange between thermal ions and an extended cloud of neutral SO2 molecules, followed by rapid dissociative recombination of the resultant molecular ion. The model provides a self-consistent solution which reconciles a number of diverse observations with known physical processes.

Moreno, M. A.↗
NASA NTRS1987

Ice growth in supercooled solutions of antifreeze glycoproteins

The effects of different degrees of supercooling on the habit and rates of growth of ice crystals from solutions of antifreeze glycoproteins are reported. To isolate the influence of different solutions and supercooling alone, a system was devised that nucleated crystals in the middle of a uniformly supercooled sample. Alternatively, single crystals of selected orientation were inserted into free liquid surface. A crystallization rate up to five times greater than that in pure water was found. A mechanism explaining these results is suggested.

Harrison, K.↗
NASA NTRS1989

Isidis basin - Site of ancient volatile-rich debris layer

The differentiation of the Martian Isidis impact basin's interior plains into hillocky terrains with isolated mounds arranged in arctuate chains, and ridged terrains with systems of parallel curvilinear ridges, is presently suggested to reflect the deposition and subsequent removal of a thick layer of material within the basin. The process of terrestrial ice-cover dissintegration, which yields such landforms as moraines, kames, and eskers, furnishes a possible analog to the Isidis features; Viking orbiter images show Martian ridges with similar characteristics, suggesting that the Isidis layer may have been only part of a more general deposition period that coincided with one of major outflow channel formation involving the release of subsurface volatiles.

Grizzaffi, Patricia↗
NASA NTRS1987

Documentation of the current fault detection, isolation and reconfiguration software of the AIPS fault-tolerant processor

Documentation is presented of the December 1986 version of the ADA code for the fault detection, isolation, and reconfiguration (FDIR) functions of the Advanced Information processing System (AIPS) Fault-Tolerant Processor (FTP). Because the FTP is still under development and the software is constantly undergoing changes, this should not be considered final documentation of the FDIR software of the FTP.

Lanning, David T.↗
NASA NTRS1990

SE and I system testability: The key to space system FDIR and verification testing

The key to implementing self-diagnosing design is a systems engineering task focused on design for testability concurrent with design for functionality. The design for testability process described here is the product of several years of DOD study and experience. Its application to the space station has begun on Work Package II under NASA and McDonnell direction. Other work package teams are being briefed by Harris Corporation with the hope of convincing them to embrace the process. For the purpose of this discussion the term testability is used to describe the systems engineering process by which designers can assure themselves and their reviewers that their designs are TESTABLE, that is they will support the downstream process of determining their functionality. Due to the complexity and density of present-day state-of-the-art designs, such as pipeline processors and high-speed integrated circuit technology, testability feature design is a critical requirement of the functional design process. A systematic approach to Space systems test and checkout as well as fault detection fault isolation reconfiguration (FDFIR) will minimize operational costs and maximize operational efficiency. An effective design for the testability program must be implemented by all contractors to insure meeting this objective. The process is well understood and technology is here to support it.

Barry, Thomas↗
NASA NTRS1991

GLObal Backscatter Experiment (GLOBE) Pacific survey mission

NASA conducted the GLObal Backscatter Experiment (GLOBE) Survey Mission over the near coastal and remote Pacific Ocean during 6 to 30 Nov. 1989 (GLOBE 1) and 13 May to 5 Jun. 1990 (GLOBE 2). These missions studied the optical, physical, and chemical properties of atmospheric aerosols. Particular emphasis was given to the magnitude and spatial variability of aerosol backscatter coefficients at mid-infrared wavelengths, and to the remote middle and upper troposphere, where these aerosol properties are poorly understood. Survey instruments were selected to provide either direct beta measurements at the key wavelengths, empirical links with long term or global scale aerosol climatologies, or aerosol microphysics data required to model any of these quantities. The survey deployment included both long distance 6 to 8 hour transit flights and detailed 4 to 6 hour local flights. Several general features were observed from preliminary Survey data analyses. Validation and intercomparison results have shown good agreement, usually better than a factor of two. Atmospheric aerosols frequently exhibited a three layer vertical structure, with (1) high and fairly uniform backscatter in the shallow cloud capped marine boundary layer; (2) moderate and highly variable backscatter in a deeper overlaying cloud pumped layer; and (3) low, regionally uniform, but seasonally and latitudinally variable backscatter in the middle and upper troposphere. The survey missions represent two isolated snapshots of a small portion of the global aerosol system. Consequently, Survey results can best be understood by synthesizing them with the more comprehensive GLOBE data base, which is being compiled at NASA-Marshall.

Bowdle, David A.↗