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At least 1,657 records · Page 92

Crystal structures of a dodecameric multicopper oxidase from Marinithermus hydrothermalis

We report multicopper oxidases (MCOs) represent a diverse family of enzymes that catalyze the oxidation of either an organic or a metal substrate with concomitant reduction of dioxygen to water. These enzymes contain variable numbers of cupredoxin domains, two, three or six per subunit, and rely on four copper ions, a single type I copper and three additional copper ions organized in a trinuclear cluster (TNC), with one type II and two type III copper ions, to catalyze the reaction. Here, two crystal structures and the enzymatic characterization of Marinithermus hydrothermalis MCO, a two-domain enzyme, are reported. This enzyme decolorizes Congo Red dye at 70°C in the presence of high halide concentrations and may therefore be useful in the detoxification of industrial waste that contains dyes. In two distinct crystal structures, MhMCO forms the trimers seen in other two-domain MCOs, but differs from these enzymes in that four trimers interact to create a dodecamer. This dodecamer of MhMCO forms a closed ball-like structure and has implications for the sequestration of bound divalent metal ions as well as substrate accessibility. In each subunit of the dodecameric structures, a Trp residue, Trp351, located between the type I and TNC sites exists in two distinct conformations, consistent with a potential role in facilitating electron transfer in the enzyme.

59 BASIC BIOLOGICAL SCIENCES↗

Direct Numerical Simulation of Involute Channel Turbulence

A direct numerical simulation (DNS) study was performed on turbulent flow in the high flux isotope reactor involute channel geometry to develop a numerical database and determine the differences compared with a flat parallel channel. The varying channel curvature along the walls was studied for differences in mean profiles. Parameters of interest include streamwise velocity, turbulent kinetic energy (TKE), and turbulence dissipation rate, as well as Reynolds stresses and turbulence transport terms. Profile sampling was carried out at 10 locations along the span of the involute. Additional DNS studies were performed on smaller domains of comparable curvature to the involute domain: a high curvature channel (high circular), a low curvature channel (low circular), and a flat channel (flat). Here, each of these four cases was compared against each other and to other DNS studies performed on parallel flows. The results indicate that the bulk involute channel flow does not differ significantly from a flat parallel channel flow and that the curvature of the walls does not significantly alter the mean flow parameters. However, the regions of the involute channel near the side walls exhibit relatively low magnitude twin recirculation structures driven toward the side walls from the centerline of the channel, which warrants further study.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Beam correction for multi-pass arcs in FFA@CEBAF: status update

This work examines the multi-pass steering of six electron beams in an FFA arc ranging from approximately 10.5 GeV to 22 GeV. Shown here is an algorithm based on singular value decomposition (SVD) to successfully steer all six beams through the arc given precise knowledge of all beam positions at each of one hundred and one diagnostic locations with one hundred individual corrector magnets: that is successive application of SVD to different 100 × 101 response matrices—one for each beam energy. Further, a machine learning scheme is developed which only requires knowledge of the energy-averaged beam position at each location to provide equivalent steering. Extension of this scheme to other beam optics quantities as well as transverse and longitudinal coupling is explored.

Accelerator Physics↗

Notice of Submittal – 2020 Radionuclide Air Emissions Report for Los Alamos National Laboratory

Enclosed is the Calendar Year 2020 Radionuclide Air Emissions Report for the Los Alamos National Laboratory (LANL). The submittal is in compliance with 40 CFR 61 Subpart H. The highest effective dose equivalent to any member of the public from LANL operations in 2020 is 0.29 millirem. This maximally exposed individual location is a business at 132 DP Road, Los Alamos, NM. The annual dose is well below the EPA standard of 10 millirem per year as established in Subpart H.

54 ENVIRONMENTAL SCIENCES↗

Effect of Mo precursors in Microwave-assisted Methane Dehydroaromatization over Mo/HZSM5 catalysts

Natural gas flaring occurs in remote shale regions due to limited pipeline takeaway capacity. The conversion of the associated natural gas into aromatics in modular microwave reactors is a viable alternative to monetize the wasted gas. Microwaves offer rapid, selective heating in compact reactor systems that can enable on-demand chemical production at the well-site. Mo-HZSM-5 catalysts are widely used for aromatic production, but the location and nature of the active sites are still under debate. This study focuses on the use of 6 different Mo precursors to elucidate insights into the Mo properties that are desirable for BTX production. The catalysts were characterized by different methods (XPS, TPR, Raman, etc) to determine differences in Mo catalytic properties that may affect performance and understand these differences through performance testing under microwave at 700C for methane dehydroaromatization. Metal precursors that enable a better distribution of Mo into the pores and over the surface lead to improved benzene yield, whereas those that limited Mo to primarily the surface suffer rapid deactivation and low benzene yields. Additionally, strong Lewis acidity that arises from the sodium containing precursor drastically shifts the product selectivity towards dehydrogenation, which produces more ethylene and carbon. This catalyst had the highest deactivation constant of all catalysts tested.

gas flaring reduction↗

On the similarity of hillslope hydrologic function: a clustering approach based on groundwater changes

Abstract. Hillslope similarity is an active topic in hydrology because of its importance in improving our understanding of hydrologic processes and enabling comparisons and paired studies. In this study, we propose a holistic bottom-up hillslope clustering based on a region's integrative hydrodynamic response quantified by the seasonal changes in groundwater levels ΔP. The main advantage of the ΔP clustering is its ability to capture recharge and discharge processes. We test the performance of the ΔP clustering by comparing it to seven other common hillslope clustering approaches. These include clustering approaches based on the aridity index, topographic wetness index, elevation, land cover, and machine-learning that jointly integrate multiple data. We assess the ability of these clustering approaches to identify and categorize hillslopes with similar static characteristics, hydroclimate, land surface processes, and subsurface dynamics in a mountainous watershed – the East River – located in the headwaters of the Upper Colorado River Basin. The ΔP clustering performs very well in identifying hillslopes with six out of the nine characteristics studied. The variability among clusters as quantified by the coefficient of variation (0.2) is less in the ΔP and the machine learning approaches than in the others (> 0.3 for TWI, elevation, and land cover). We further demonstrate the robustness of the ΔP clustering by testing its ability to predict hillslope responses to wet and dry hydrologic conditions, of which it performs well when based on average conditions.

54 ENVIRONMENTAL SCIENCES↗

Integrated engine generator for aircraft secondary power

An integrated engine-generator for aircraft secondary power generation is described. The concept consists of an electric generator located inside a turbojet or turbofan engine and both concentric with and driven by one of the main engine shafts. The electric power conversion equipment and generator controls are located in the aircraft. When properly rated, the generator serves as an engine starter as well as a source of electric power. This configuration reduces or eliminates the need for an external gear box on the engine and permits reduction in the nacelle diameter.

Secunde, R. R.↗

Integrated engine-generator for aircraft secondary power.

The integrated engine-generator concept consists of an electric generator located inside a turbojet or turbofan engine and both concentric with and driven by one of the main engine shafts. The electric power-conversion equipment and generator controls are conveniently located in the aircraft. When properly rated, the generator serves as an engine starter as well as a source of electric power. The available generating capacity permits use of electrically driven engine accessories. This reduces or eliminates the need for an external gearbox on the engine, thereby simplifying the engine and nacelle assembly and increasing aircraft design flexibility. The nacelle diameter can then be decreased, resulting in less aerodynamic drag and reduced takeoff gross weight.

Secunde, R. R.↗

Effects of the air breathing propulsion system on space shuttle orbiter subsonic stability and control characteristics (OA71A)

Experimental aerodynamic investigations were conducted on an 0.0405 scale representation of the -89B (2A) Space Shuttle Orbiter in a 7.75 x 11.00 ft low speed wind tunnel during the time period from July 27, 1973 to August 3, 1973. The primary test objective was to investigate the aerodynamic effects of engine nacelle grouping and location on the orbiter ferry mission configuration. Five nacelles were tested, both individually mounted as well as mounted in a podded configuration, at the baseline position and moved 45.0 in. aft (full scale). Orbiter control effectiveness, both with and without nacelles, was recorded at elevon deflections of 0 deg, 5 deg, 10 deg, -10 deg and -20 deg and aileron deflections, about 0 deg elevon, of 0 deg, 5 deg, 10 deg, and 15 deg. The model was sting mounted on a 2.5 inch diameter internal strain gage balance entering through the base region. The nominal angle of attack range was -4 deg or = alpha or = 30 deg. Yaw polars were recorded over the beta range of -10 deg or = beta or = at fixed angles of attack of 0 deg and 10 deg.

Mennell, R. C.↗

Catalog of data summary of airglow observations obtained at Haleakala, Hawaii

The airglow studies were initiated in July 1961 in response to the discovery of unusual phenomena in the tropical airglow as observed from Algeria. Hawaii, being the southern-most area in the United States and within the tropics, and possessing high mountains with frequent clear skies well isolated from the contaminating light and dust of civilization, appeared to be an ideal location for establishing a tropical airglow station. The University of Hawaii was already developing the Mees Solar Laboratory on Haleakala on the island of Maui, so that the addition of a night sky observatory was a natural development. The collection of data covers a span of about seven and one-half years, including the minimum of the solar cycle in 1963 and just getting into the next maximum in 1968-69. It was unfortunate that financial limitations did not permit the continuation of the observations through one complete solar cycle.

Steiger, W. R.↗

Monitoring the vernal advancement and retrogradation (green wave effect) of natural vegetation

The author has identified the following significant results. From the data that have been analyzed thus far, it is apparent that certain parameters of the vegetation (green biomass and vegetation moisture content) are well correlated with ERTS data at some sites, yet the relationship is poor at other locations. The analyses indicate that the green wave development (spring) can be readily detected in the Great Plains Corridor. Consequently, ERTS-1 data provide a new tool for monitoring range readiness on a regional basis. The onset of summer drought and its duration can also be monitored. Data from ten G.P.C. sites suggest that the satellite coverage has been adequate to monitor the status of rangeland vegetation for regional management purposes.

Rouse, J. W., Jr.↗

Variable positron annihilation radiation from the galactic center region

HEAO 3 Cosmic Gamma-Ray Spectrometer evidence is presented for the existence of a time-varying, unshifted, narrow 511 keV line emission from the vicinity of the galactic center. Although uncertainties exist regarding the spatial extent of the features as well as its centroid, all data are consistent with emission from a single point source located at the galactic center. This interpretation would require a source luminosity of 2 x 10 to the 37th ergs/sec, and a positron annihilation rate of about 10 to the 43rd/sec. It is concluded that a variable source of positrons which could generate such an annihilation figure might be a massive black hole at the galactic center, as has been suggested by IR observations.

Riegler, G. R.↗

Physics of the interaction of the solar wind with the ionosphere of Venus - Flow/field models

Venus deflects incident solar wind by means of thermal and magnetic pressure, both of which are produced by ionospheric currents that are in turn driven by pressure gradients and electric fields induced in the ionosphere by the flowing magnetized solar wind plasma. The ionospheric currents connect across the ionopause into the ionosheath region, and close on, and behind, the bow shock. The computed ionospheric plasma velocity distribution is found to be spatially asymmetric, with the fastest flow velocities lying perpendicular to the ionospheric magnetic fields. Computations also indicate that the ionosphere is susceptible to an MHD shear instability whose extent and location is a function of external solar wind conditions. The present Venus ionosphere model compares well with in situ Pioneer Venus Orbiter observations.

Cloutier, P. A.↗

Analytical model of rotor wake aerodynamics in ground effect

The model and the computer program developed provides the velocity, location, and circulation of the tip vortices of a two-blade helicopter in and out of the ground effect. Comparison of the theoretical results with some experimental measurements for the location of the wake indicate that there is excellent accuracy in the vicinity of the rotor and fair amount of accuracy far from it. Having the location of the wake at all times enables us to compute the history of the velocity and the location of any point in the flow. The main goal of out study, induced velocity at the rotor, can also be calculated in addition to stream lines and streak lines. Since the wake location close to the rotor is known more accurately than at other places, the calculated induced velocity over the disc should be a good estimate of the real induced velocity, with the exception of the blade location, because each blade was replaced only by a vortex line. Because no experimental measurements of the wake close to the ground were available to us, quantitative evaluation of the theoretical wake was not possible. But qualitatively we have been able to show excellent agreement. Comparison of flow visualization with out results has indicated the location of the ground vortex is estimated excellently. Also the flow field in hover is well represented.

Saberi, H. A.↗

Capabilities and limitations of the Jicamarca radar as an MST radar

The Jicamarca radar (Long. 76.52W, Lat. 11.56S), located at 20 km from Lima at approximately 500 meters over sea level, is surrounded by mountains which provide a good shield from man-made interference. The radio horizon goes from a few hundred meters, across the dry valley where it is located, to 15 km, along the valley in the direction of the continental divide. This limits the clutter to 15 km, except for one high peak at 21 km. It is the most equatorial of all existing MST radars. Its proximity to the Andes, makes its location unique for the study of lee waves and orographic-induced turbulence. Vertical as well as horizontal projections of MST velocities are obtained by simultaneously pointing with different sections of the antenna into three or four different directions. The transmitters, receivers, and systems for data acquisition, processing, and control are included.

Woodman, R. F.↗

Optoelectronic Proximity Sensor Finds Edges

Seams tracked for automatic control of welding. Optoelectronic system based on relatively simple array of optical proximity sensors locates edge of plate. Used to control automatic production machinery. System follows curved seams as well as straight ones, and neither special control programming nor precise initial positioning workpiece necessary for accurate tracking of seam.

Bejczy, Antal K.↗

Modified independent modal space control method for active control of flexible systems

A modified independent modal space control (MIMSC) method is developed for designing active vibration control systems for large flexible structures. The method accounts for the interaction between the controlled and residual modes. It incorporates also optimal placement procedures for selecting the optimal locations of the actuators in the structure in order to minimize the structural vibrations as well as the actuation energy. The MIMSC method relies on an important feature which is based on time sharing of a small number of actuators, in the modal space, to control effectively a large number of modes. Numerical examples are presented to illustrate the application of the method to generic flexible systems. The results obtained suggest the potential of the devised method in designing efficient active control systems for large flexible structures.

Baz, A.↗