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At least 1,639 records · Page 91

Pr-specific phytochrome phosphorylation in vitro by a protein kinase present in anti-phytochrome maize immunoprecipitates

Protein kinase activity has repeatedly been found to co-purify with the plant photoreceptor phytochrome, suggesting that light signals received by phytochrome may be transduced or modulated through protein phosphorylation. In this study immunoprecipitation techniques were used to characterize protein kinase activity associated with phytochrome from maize (Zea mays L.). A protein kinase that specifically phosphorylated phytochrome was present in washed anti-phytochrome immunoprecipitates of etiolated coleoptile proteins. No other substrate tested was phosphorylated by this kinase. Adding salts or detergents to disrupt low-affinity protein interactions reduced background phosphorylation in immunoprecipitates without affecting phytochrome phosphorylation, indicating that the protein kinase catalytic activity is either intrinsic to the phytochrome molecule or associated with it by high-affinity interactions. Red irradiation (of coleoptiles or extracts) sufficient to approach photoconversion saturation reduced phosphorylation of immunoprecipitated phytochrome. Subsequent far-red irradiation reversed the red-light effect. Phytochrome phosphorylation was stimulated about 10-fold by a co-immunoprecipitated factor. The stimulatory factor was highest in immunoprecipitates when Mg2+ was present in immunoprecipitation reactions but remained in the supernatant in the absence of Mg2+. These observations provide strong support for the hypothesis that phytochrome-associated protein kinase modulates light responses in vivo. Since only phytochrome was found to be phosphorylated, the co-immunoprecipitated protein kinase may function to regulate receptor activity.

NASA Discipline Plant Biology↗

Climate-Related Trends of Within-Storm Intensities Using Dimensionless Temporal-Storm Distributions

Huff curves are probabilistic time distributions of rainfall expressed as dimensionless cumulative percentages of storm depth and duration. Previous studies have documented development factors, spatial robustness, and the utility of Huff curves in practical applications. However, the effects of trending rainfall on Huff curve intensity patterns have not yet been studied. As such, the goal of this paper is to fill this gap by studying Huff curve patterns in a watershed with demonstrated increasing trends of temperature and precipitation, with the intention that it can be generalized to other areas in the US and the world. To achieve this goal, the high temporal resolution precipitation data collected from a high spatial density, 72-year precipitation-gauge network on the 4.25-km 2 North Appalachian Experimental Watershed in east-central Ohio were used. Seasonal storm pattern trends from 1939 to 2010 were investigated using dimensionless depth (with the frequency of 50%, $d_{50}$) and the curve variability $(V = d_{80} - d_{20})$ at three dimensionless within-storms time periods (three verticals). The Spearman rank correlation procedure (correlation coefficient, ρ and significance probability, p) was used to statistically determine trends over time using 8 periods of 4-season sets of Huff curves over the 72 years. Two averages of ρ and p were computed: (1) by averaging the individual ρ and p obtained from 10 gauges (AvgI); and (2) by grouped averaging of all individual gauge values of $d_{50}$ and V and then computing ρ and p (AvgG). The test results of individual gauges showed that 4 cases for $d_{50}$ and 23 cases for V were significant for all seasons and verticals (total of 120 cases for each variable). The test results of AvgI for $d_{50}$ and V and AvgG for $d_{50}$ showed no significant trends in all seasons and verticals. Only the AvgG for V led to a significant trend for V in spring and fall at different times within storm patterns. The data do not provide sufficient evidence at the p=0.05 significance level to reject the null hypothesis of unchanging position of the dimensionless depth of the 50% Huff curves for individual or averages for all seasons and verticals. Also, there is insufficient evidence to reject the null hypothesis of unchanging variability, V, using the average ρ of individual gauges (AvgI for V) for all seasons and verticals. AvgG results showed a significant trend in V; however, this analysis may be affected by the nonindependence of storm data. The results suggest that it is likely that there is little if any effect of trending climate over approximately 70 years on Huff curve patterns. The results of this study add to the robustness characteristics of Huff curves and to their potential use in hydrological practice as design storms, as the foundation of stochastic storm generation, and for storm disaggregation, and they deserve further investigation. These different forms of inputs to watershed models have the potential to improve runoff estimation. The results suggest that, if verified in other studies, they have applicability to provide useful stationary precipitation patterns across the US and other areas of the world in areas of nonstationary climate. Also, individual rain gauge data may not be representative of trends even over small areas, and seasonal differences were noticeable as found in other studies. Recommendations are provided.

54 ENVIRONMENTAL SCIENCES↗

Measuring the Muon Neutrino Magnetic Moment in the NOvA Near Detector

Measuring an enhanced neutrino magnetic moment would be a clear indication of physics beyond the Standard Model, shedding light on the correct beyond Standard Model theory or the potential Majorana nature of neutrinos. It would manifest in the NOvA near detector as an excess of neutrino-on-electron elastic scattering interactions at low electron recoil energies. Leveraging an intense and highly pure muon neutrino beam, along with a finely segmented liquid scintillator detector technology specifically designed for electromagnetic shower separation, enables NOvA to achieve a potentially world-leading sensitivity in probing the effective muon neutrino magnetic moment. This analysis, based on neutrino data collected between 2014 and 2021, corresponding to an exposure of $13.8\times10^{20}$ protons-on-target, yields a result consistent with the Standard Model-only hypothesis with a p-value of 0.31. An upper limit on the effective muon neutrino magnetic moment is set at $\mu_{\nu_\mu}<19.1\times 10^{-10}\mu_B$ at $90\%$ confidence level. Despite facing statistical limitations stemming from low cross section of the signal process, systematic uncertainties have a significant impact on this result. To address these challenges, the NOvA Test Beam experiment focuses on mitigating some of the largest systematic uncertainties within NOvA by investigating particle interactions and energy deposition in a small-scale replica NOvA detector. This thesis describes the calibration of the NOvA Test Beam detector, which is a crucial step in analysing the Test Beam data before they can be utilised to reduce NOvA systematic uncertainties.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Innovating the next generation of commercial smart building software

Nearly 30% of commercial building energy use is wasted due to equipment faults and HVAC controls problems. The result is increased emissions, compromised comfort and productivity, and less reliable coordination of building power needs with a clean grid. The energy impact alone represents $17 billion in potential savings. Today’s smart building software provides a robust solution to address these operational deficiencies. Energy management and information systems (EMIS) are saving up to 9% on average, with two-year paybacks. They are being incorporated into energy management processes, commissioning services, and utility programs. As effective as they are, two barriers prevent even deeper benefits; limited personnel to fix problems once they are identified, and the expense and time to manually implement changes in control systems. In partnership with the research community, the EMIS industry is developing new capabilities to overcome these barriers. Moving beyond siloed products for either fault detection and diagnostics, or optimal control, these new capabilities empower users to not only automatically identify faults, but also to push corrective action, and control improvements to their buildings. In this paper, several areas for enhancements are documented: ‘one-time’ correction of faults such as setpoints, schedules, and economizer lockouts; short-term active testing for automated proportional integral derivative (PID) loop tuning and functional testing; and continuous supervisory control for demand flexibility and year-round efficiency. Results are presented from a pair of partner implementations out of a dozen providers integrating these enhancements into their products, including field tests from across the country, and insights into operator acceptance and integration into operations and maintenance practices.

Casillas, Armando↗

Noncoplanar magnetic fields at collisionless shocks - A test of a new approach

The expressions derived by Jones and Ellison (1987) (JE) for the spatial integral of the noncoplanar field component in a fast-mode collisionless shock and for the magnitudes of the magnetic-field rotation and potential drop difference are evaluated on the basis of ISEE 1 and 2 observations in the earth bow shock and numerical simulations using the hybrid code of Leroy et al. (1981 and 1982). The data and simulation results are presented graphically and shown to be consistent with the JE hypothesis, that the rotation and drop difference are due to unequal electron and ion masses. The rotations and potential drop differences predicted by the JE equations, however, are found to be accurate only for subcritical shocks at low Mach numbers; the underestimation of the values for hypercritical shocks is attributed to neglect of the ion current from reflected gyrating ions.

Gosling, J. T.↗

Numerical study of the current sheet and PSBL in a magnetotail model

The current sheet and plasma sheet boundary layer (PSBL) in a magnetotail model are discussed. A test particle code is used to study the response of ensembles of particles to a two-dimensional, time-dependent model of the geomagnetic tail, and test the proposition (Coroniti, 1985a, b; Buchner and Zelenyi, 1986; Chen and Palmadesso, 1986; Martin, 1986) that the stochasticity of the particle orbits in these fields is an important part of the physical mechanism for magnetospheric substorms. The realistic results obtained for the fluid moments of the particle distribution with this simple model, and their insensitivity to initial conditions, is consistent with this hypothesis.

Doxas, I.↗

A near-wall two-equation model for compressible turbulent flows

A near-wall two-equation turbulence model of the K - epsilon type is developed for the description of high-speed compressible flows. The Favre-averaged equations of motion are solved in conjunction with modeled transport equations for the turbulent kinetic energy and solenoidal dissipation wherein a variable density extension of the asymptotically consistent near-wall model of So and co-workers is supplemented with new dilatational models. The resulting compressible two-equation model is tested in the supersonic flat plate boundary layer - with an adiabatic wall and with wall cooling - for Mach numbers as large as 10. Direct comparisons of the predictions of the new model with raw experimental data and with results from the K - omega model indicate that it performs well for a wide range of Mach numbers. The surprising finding is that the Morkovin hypothesis, where turbulent dilatational terms are neglected, works well at high Mach numbers, provided that the near wall model is asymptotically consistent. Instances where the model predictions deviate from the experiments appear to be attributable to the assumption of constant turbulent Prandtl number - a deficiency that will be addressed in a future paper.

Zhang, H. S.↗

Copoly(imide siloxane) Abhesive Materials with Varied Siloxane Oligomer Length

In this work, low surface energy copoly(imide siloxane)s were synthesized with various siloxane segment lengths. Characterization of these materials revealed that domain formation of the low surface energy component within the matrix was more prevalent for longer siloxane segments as indicated by increased opacity, decreased mechanical properties, and variation of the Tg. Incorporation of siloxanes lowered the polymer s surface energy as indicated by water contact angle values. Topographical modification of these materials by laser ablation patterning further reduced the surface energy, even generating superhydrophobic surfaces. Combined, the contact angle data and particle adhesion testing indicated that copoly(imide siloxane) materials may provide greater mitigation to particulate adhesion than polyimide materials alone. These enhanced surface properties for abhesive applications did result in a reduction of the tensile moduli of the copolymers. It is possible that lower siloxane loading levels would result in retention of the mechanical properties of the polyimide while still affording abhesive surface properties. This hypothesis is currently being investigated. Laser ablation patterning offers further reduction in particle retention as the available surface area for particle adhesion is reduced. Pattern variation and size dependencies are currently being evaluated. For the purposes of lunar dust adhesion mitigation, it is likely that this approach, termed passive due to the lack of input from an external energy source, would not be sufficient to mitigate surface contamination or clean contaminated surfaces for some lunar applications. It is feasible to combine these materials with active mitigation strategies - methods that utilize input from external energy sources - would broaden the applicability of such materials for abhesive purposes. Collaborative efforts along these lines have been initiated with researchers at NASA Kennedy Space Center where experiments are being conducted involving a series of embedded electrodes within polymeric matrices.

Wohl, Christoper J.↗

Turbulent kinetic energy equation and free mixing

Calculation of free shear flows was carried out to investigate the usefulness of several concepts which were previously successfully applied to wall flows. The method belongs to the class of differential approaches. The turbulence is taken into account by the introduction of one additional partial differential equation, the transport equation for the turbulent shear stress. The structure of turbulence is modeled after Bradshaw et al. This model was used successfully in boundary layers and its applicability to other flows is demonstrated. The work reported differs substantially from that of an earlier attempt to use this approach for calculation of free flows. The most important difference is that the region around the center line is treated by invoking the interaction hypothesis (concerning the structure of turbulence in the regions separated by the velocity extrema). The compressibility effects on shear layer spreading at low and moderate Mach numbers were investigated. In the absence of detailed experiments in free flows, the evidence from boundary layers that at low Mach numbers the structure of turbulence is unaffected by the compressibility was relied on. The present model was tested over a range of self-preserving and developing flows including pressure gradients using identical empirical input. The dependence of the structure of turbulence on the spreading rate of the shear layer was established.

Morel, T.↗

Mass mortality and extraterrestrial impacts

The discovery of iridium enrichment at the Cretaceous/Tertiary boundary resulted in formulation of hypothesis of a cometary or asteroid impact as the cause of the biological extinctions at this boundary. Subsequent discoveries of geochemical anomalies at major stratigraphic boundaries like the Precambrian/Cambrian, Permian/Triassic, Middle/Late Jurassic, resulted in the application of similar extraterrestrial impact theories to explain biological changes at these boundaries. Until recently the major physical evidence, as is the location of the impact crater site, to test the impact induced biological extinction was lacking. The diameter of such a crater would be in the range of 60 to 100 km. The recent discovery of the first impact crater in the ocean provide the first opportunity to test the above theory. The crater, named Montagnais and located on the outer shelf off Nova Scotia, Canada, has a minimum diameter of 42 km, with some evidence to a diameter of more than 60 km. At the Montagnais impact site, micropaleontological analysis of the uppermost 80 m of the fall-back breccia represented by a mixture of pre-impact sediments and basement rocks which fills the crater and of the basal 50 m of post-impact marine sediments which overly the impact deposits, revealed presence of diversified foraminiferal and nannoplankton assemblages. The sediments which are intercalated within the uppermost part of the fall-back breccia, had to be deposited before the meteorite impact. The post-impact deposits were laid down almost immediately after the impact as also supported by the micropaleontological data. In conclusion, micropaleontological studies of sediments from the first submarine impact crater site identified in the ocean did not reveal any mass extinction or significant biological changes at the impact site or in the proximal deep ocean basin.

Jansa, L. F.↗

Evolution versus pulsation along the horizontal branch of M15

The study combines the horizontal-branch tracks (HBETs) of Lee and Demarque (1990) with the period/mean density law of pulsation theory in order to account for the observed periods and relative luminosities of 62 RR Lyrae stars in the extreme metal-poor globular cluster M15. These tests were performed for a number of assumed values for the mean luminosity of the M15 RR Lyrae sample and for different assumed boundaries for the pulsational instability strip. It was found that no single set of models could account for all the RR Lyrae periods and luminosities while simultaneously reproducing the observed value for the the ratio of blue to variable stars. The hypothesis that an RR Lyrae sample evolved near helium exhaustion along the HBETs cannot explain the relatively large number of variable stars in M15 is confirmed.

Simon, Norman R.↗

A near-wall two-equation model for compressible turbulent flows

A near-wall two-equation turbulence model of the k-epsilon type is developed for the description of high-speed compressible flows. The Favre-averaged equations of motion are solved in conjunction with modeled transport equations for the turbulent kinetic energy and solenoidal dissipation wherein a variable density extension of the asymptotically consistent near-wall model of So and co-workers is supplemented with new dilatational models. The resulting compressible two-equation model is tested in the supersonic flat plate boundary layer - with an adiabatic wall and with wall cooling - for Mach numbers as large as 10. Direct comparisons of the predictions of the new model with raw experimental data and with results from the K-omega model indicate that it performs well for a wide range of Mach numbers. The surprising finding is that the Morkovin hypothesis, where turbulent dilatational terms are neglected, works well at high Mach numbers, provided that the near wall model is asymptotically consistent. Instances where the model predictions deviate from the experiments appear to be attributable to the assumption of constant turbulent Prandtl number - a deficiency that will be addressed in a future paper.

Zhang, H. S.↗

Are the 1986-1988 changes in solar free-oscillation frequency splitting significant?

The solar normal-mode splitting coefficients deduced from Big Bear Solar Observatory (BBSO) data differ between 1986 and 1988; inversions for equatorial rotation are slower at depth and faster near the surface in 1988 than in 1986. The significance of the change has been disputed. The data sets overlap for five splitting coefficients (a(j))super 5 sub j = 1 associated with 710 multiplets. On the assumption that rotation rate varies smoothly with radius, both data sets are satisfied by the same rotation model at all colatitudes except near 30-40 deg and near 70 deg (and at their southern hemisphere reflections 140-150 deg and 110 deg). The evidence for equatorial change is weak. Nonparametric tests show a significant offset in the magnitudes of a(1), a(2), and a(4), and of linear combinations sensitive to rotation at colatitudes of 60-80 deg (and 120 deg). Nonparametric tests show significant radial trends in the changes to a(2), a(4), and (less significantly) a(5). There is strong anticorrelation between a(2) and a(4), a(1) and a(3), and a(3) and a(5), suggesting that the estimates are not independent. Individual coefficients a(j) show more evidence for change than do 'physical' linear combinations, adding weight to this hypothesis. Some of the changes in splitting might be related to solar activity, which changed most near colatitude 70 deg from 1986 to 1988.

Gough, Douglas↗

The Evaluation of Cryogenic Force Balance Calibration Methodologies with Respect to Wind Tunnel Results

Independent tests of the NASA Common Research Model (CRM) at NASA's National Transonic Facility (NTF) and the European Transonic Windtunnel (ETW) revealed discrepancies at low operating temperatures and high Reynolds numbers that warranted further investigation. Since each facility used their own force balance for their tunnel entry, one suggestion for the discrepancy was the temperature compensation methodology developed and applied for each balance. This hypothesis is explored through simulation and experimentally. Independent calibrations of NASA's NTF-118A balance at NASA Langley and ETW reveal discrepancies in the thermal compensation of the normal force and pitching moment primary sensitivities with temperature, while the axial force primary sensitivities are in good agreement. The application of the force balance calibrations performed at NASA and ETW to the prior wind tunnel data suggests that the thermal compensation discrepancies are an order of magnitude less than the discrepancies observed between the wind tunnel aerodynamic coefficients.

Burns, Devin E.↗

Distributed Inference with Sparse and Quantized Communication

Here, we consider the problem of distributed inference where agents in a network observe a stream of private signals generated by an unknown state, and aim to uniquely identify this state from a finite set of hypotheses. We focus on scenarios where communication between agents is costly, and takes place over channels with finite bandwidth. To reduce the frequency of communication, we develop a novel event-triggered distributed learning rule that is based on the principle of diffusing low beliefs on each false hypothesis. Building on this principle, we design a trigger condition under which an agent broadcasts only those components of its belief vector that have adequate innovation, to only those neighbors that require such information. We prove that our rule guarantees convergence to the true state exponentially fast almost surely despite sparse communication, and that it has the potential to significantly reduce information flow from uninformative agents to informative agents. Next, to deal with finite-precision communication channels, we propose a distributed learning rule that leverages the idea of adaptive quantization. We show that by sequentially refining the range of the quantizers, every agent can learn the truth exponentially fast almost surely, while using just 1 bit to encode its belief on each hypothesis. For both our proposed algorithms, we rigorously characterize the trade-offs between communication-efficiency and the learning rate.

42 ENGINEERING↗

Heart Rate Variability During Early Adaptation to Space

A recent report hypothesized that episodes of space motion sickness (SMS) were reliably associated with low frequency oscillations (less than 0.03 to less than 0.01 Hz) in heart rate variability. This paper archives a large data set for review of investigators in this field which may facilitate the evaluation of this hypothesis. Continuous recording of Electro-cardiography (ECG) and other measures were made for 6 to 12 hours per day (waking hours) of six Shuttle crewmembers for the first 3 mission days of two separate Shuttle flights. Spectral analyses of heart rate variability during approximately 200 hours of inflight is presented. In addition, nearly 200 hours of data collected on these same individuals during ground tests prior to the mission are presented. The Purpose of this Publication is to document the incidence of low frequency oscillations of heart rate in 4 people exposed to microgravity over a period of five days. In addition, this report contains spectral analyses of heart rate data collected on these same individuals during ground-based mission simulations. By archiving these data in this manner, it is our intention to make this information available to other investigators interested in studying this phenomena.

Toscano, W. B.↗

Crustal Deformation and the Seismic Cycle across the Kodiak Islands, Alaska

The Kodiak Islands are located approximately 130 to 250 km from the Alaska-Aleutian Trench where the Pacific plate is underthrusting the North American plate at a rate of about 57 mm/yr. The southern extent of the 1964 Prince William Sound (${M-w}$ = 9.2) earthquake rupture occurred offshore and beneath the eastern portion of the Kodiak Islands. Here we report GPS results (1993-2001) from northern Kodiak Island that span the transition between the 1964 uplift region along the eastern coast and the region of coseismic subsidence further inland. The horizontal velocity vectors range from 22.9 $\pm$ 2.2 mm/yr at N26.3$\deg$W $\pm$ 2.5$\deg$, about 150 km from the trench, to 5.9 $\pm$ 1.3 mm/yr at N65.9$\deg$W $\pm$ 6.6$\deg$, about 190 km from the trench. Near the northeastern coast of Kodiak the velocity vector above the shallow, locked main thrust zone is between the orientation of PCFC-NOAM plate motion (N22$/deg$W) and the trench-normal (N3O$\deg$W). Further west, our geodetic results suggest the accumulation of shear strain that will be released eventually as left-lateral motion on upper plate faults such as the Kodiak Island fault. These results are consistent with the hypothesis that the difference between the Pacific-North American plate motion and the orientation of the down going slab would lead to 4-8 mm/yr of left-lateral slip. Short-term geodetic uplift rates range from 2 - 14 mm/yr, with the maximum uplift located near the axis of maximum subsidence during the 1964 earthquake. We evaluated alternate interseismic models for Kodiak to test the importance of various mechanisms responsible for crustal deformation rates. These models are based on the plate interface slip history inferred from earlier modeling of coseismic and post-seismic geodetic results. The horizontal (trench perpendicular) and vertical deformation rates across Kodiak are consistent with a model that includes the viscoelastic response to : (1) a downgoing Pacific plate interface that is locked at shallow depths,(2) coseismic slip in the 1964 and (3) interseismic creep below the seismogenic zone. The change in orientation of the horizontal velocity vector occurs down-dip from the locked main thrust zone. In southern Kodiak, the coseismic slip in the 1964 earthquake was smaller than in the northern Kodiak region; yet, the horizontal, interseismic velocities as a function of distance from the trench are comparable to those in northern Kodiak. Based on the earthquake history prior to, and following the 1964 earthquake, we hypothesize that the plate interface in southern Kodiak slips in more frequent large earthquakes than in northern Kodiak.

Sauber, Jeanne↗

Low-cost sensors and analytics for a digital factory

ORNL Manufacturing Demonstration Facility and Perisense worked on a low-cost solution for machining process monitoring to enable a digital factory. Enabling machine connectivity for digital factors is expensive for many small to medium manufacturers. The hypothesis for the research project is that a lowcost sensor suite, which can be retrofitted to a legacy machine tool or a new machine tool, can effectively provide real-time, in-situ sensing for manufacturing processes, which can enable improvements in process performance and efficiency. The Perisense sensor node was installed on the Haas TM1 machine with a current sensor and an accelerometer. Cutting tests were performed at different process parameters and sensor data was recorded. Results show a correlation of the current sensor to the spindle load. The accelerometer, mounted on the machine base, showed a correlation to the spindle speed as well as the spindle load. The sensor data can be used to monitor the machining process and provide insights on the machine spindle load utilization.

42 ENGINEERING↗