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At least 1,621 records · Page 90

Egr1 is a 3D matrix–specific mediator of mechanosensitive stem cell lineage commitment

While extracellular matrix (ECM) mechanics strongly regulate stem cell commitment, the field’s mechanistic understanding of this phenomenon largely derives from simplified two-dimensional (2D) culture substrates. Here, we found a 3D matrix–specific mechanoresponsive mechanism for neural stem cell (NSC) differentiation. NSC lineage commitment in 3D is maximally stiffness sensitive in the range of 0.1 to 1.2 kPa, a narrower and more brain-mimetic range than we had previously identified in 2D (0.75 to 75 kPa). Transcriptomics revealed stiffness-dependent up-regulation of early growth response 1 (Egr1) in 3D but not in 2D. Egr1 knockdown enhanced neurogenesis in stiff ECMs by driving β-catenin nuclear localization and activity in 3D, but not in 2D. Mechanical modeling and experimental studies under osmotic pressure indicate that stiff 3D ECMs are likely to stimulate Egr1 via increases in confining stress during cell volumetric growth. To our knowledge, Egr1 represents the first 3D-specific stem cell mechanoregulatory factor.

59 BASIC BIOLOGICAL SCIENCES↗

Soil pore network response to freeze-thaw cycles in permafrost aggregates

This dataset contains data used for the paper "Pore network response to freeze-thaw cycles in permafrost aggregates". The Related References field will be updated with a full citation when available.Climate change in Arctic landscapes may increase freeze-thaw frequency within the active layer as well as newly thawed permafrost. A highly disruptive process, freeze-thaw can deform soil pores and alter the architecture of the soil pore network with varied impacts to water transport and retention, redox conditions, and microbial activity. Our objective was to investigate how freeze-thaw cycles impacted the pore network of newly thawed permafrost aggregates to improve understanding of what type of transformations can be expected from warming Arctic landscapes. We measured the impact of freeze-thaw on pore morphology, pore throat diameter distribution, and pore connectivity with X-ray computed tomography (XCT) using six permafrost aggregates with sizes of 2.5 cm3 from a mineral soil horizon (Bw; 28-50 cm depths) in Toolik, Alaska. Freeze-thaw cycles were performed using a laboratory incubation consisting of five freeze-thaw cycles (-10˚C to 20˚C) over five weeks. Our findings indicated decreasing spatial connectivity of the pore network across all aggregates with higher frequencies of singly connected pores following freeze-thaw. Water-filled pores that were connected to the pore network decreased in volume while the overall connected pore volumetric fraction was not affected. Shifts in the pore throat diameter distribution were mostly observed in pore throats ranges of 100 microns or less with no corresponding changes to the pore shape factor of pore throats. Responses of the pore network to freeze-thaw varied with aggregate, suggesting that initial pore morphology may play a role in driving freeze-thaw response. Our research suggests that freeze-thaw alters the microenvironment of permafrost aggregates during the incipient stage of deformation following permafrost thaw, impacting soil properties and function in Arctic landscapes undergoing transition. This dataset contains a compressed (.zip) archive of the data and R scripts used for this manuscript. The dataset includes files in .csv format, which can be accessed and processed using MS Excel or R. This archive can also be accessed on GitHub at https://github.com/Erin-Rooney/XCT-freezethaw (DOI: 10.5281/zenodo.5816355).

54 ENVIRONMENTAL SCIENCES↗

High-Temperature and High-Pressure In Situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Nuclear magnetic resonance (NMR) spectroscopy represents an important technique to understand the structure and bonding environment of molecules. There exists a drive to conduct characterization experiment under conditions relevant to the chemical process of interest. To address this, in situ high-temperature, high-pressure MAS NMR methods have been developed to enable the observation of chemical interactions over a range of pressures (vacuum to several hundred bar) and temperatures (well below 0°C to 250°C). Further, the chemical identity of the samples can be comprised of solids, liquids, and gases or mixtures of the three. The method incorporates all-zirconia NMR rotors (sample holder for MAS NMR) which can be sealed using a threaded cap to compress an O-ring. This rotor exhibits great chemical resistance, temperature compatibility, low NMR background, and can withstand high pressures. These factors combined enable its use in a wide range of system combinations which permit its use in diverse fields as carbon sequestration, catalysis, material science, geochemistry, and biology. The flexibility of this technique makes it an attractive option for scientists from numerous disciplines.

Nuclear Magnetic Resonance, NMR, in situ, spectros↗

Experimental determination of the admittances of aluminized propellants by the impedance tube method

The adaptation of the impedance tube concept for the determination of the pressure coupled admittances and response functions of burning solid propellants is discussed. The results obtained in experiments with UTP-3001 and UTP-19360 aluminized propellants are presented. It is shown that the admittance Y remains constant during the quasi-steady burn period of a test, indicating constant driving of the gas phase disturbance by the burning solid propellant. The measured real part of the admittance is positive, indicating that the burning aluminized propellant is driving the gas phase oscillations. In addition, the measured high gas phase damping, provided by the aluminum oxide particles in the gas phase, suggests that the latter can significantly increase the damping in unstable solid rockets over the investigated frequency range. Finally, it is shown that the wave structure obtained by numerically solving the impedance tube wave equations which utilize the determined propellant admittance as an initial condition and the determined value of G to describe the gas phase losses is in excellent agreement with the measured wave structure.

Baum, J. D.↗

Measurement of pressure ridges in SAR images of sea ice - Preliminary results on scattering theory

Sea ice ridges and keels (hummocks and bummocks) are important in sea ice research for both scientific and practical reasons. A long-term objective is to make quantitative measurements of sea ice ridges using synthetic aperture radar (SAR) images. The preliminary results of a scattering model for sea ice ridge are reported. The approach is through the ridge height variance spectrum Psi(K), where K is the spatial wavenumber, and the two-scale scattering model. The height spectrum model is constructed to mimic height statistics observed with an airborne optical laser. The spectrum model is used to drive a two-scale scattering model. Model results for ridges observed at C- and X-band yield normalized radar cross sections that are 10 to 15 dB larger than the observed cross sections of multiyear ice over the range of angles of incidence from 10 to 70 deg.

Vesecky, J. F.↗

SORCE and Future Satellite Observations of Solar Irradiance

With solar activity just passing the maximum of cycle 23, SORCE is beginning a 5 year mission to measure total solar irradiance (TSI) with unprecedented accuracy using phase-sensitive detection, and to measure spectral solar irradiance (SSI) with unprecedented spectral coverage, from 1 to 2000 nm. The new Total Irradiance Monitor (TIM) has 4 active cavity radiometers, any one of which can be used as a fixed-temperature reference against any other that is exposed to the Sun via a shutter that cycles at a rate designed to minimize noise at the shutter frequency. The new Spectral Irradiance Monitor (SIM) is a dual Fery prism spectrometer that can employ either prism as a monochromatic source on the other prism, thus monitoring its transmission during the mission lifetime. Either prism can measure SSI from 200 to 2000 nm, employing the same phase-sensitive electrical substitution strategy as TIM. SORCE also carries dual SOLSTICE instruments to cover the spectral range 100-320 nm, similar to the instruments onboard UARS, and also an XUV Photometer System (XPS) similar to that on TIMED. SSI has now been added to TSI as a requirement of EOS and NPOESS, because different spectral components drive different components of the climate system - UV into upper atmosphere and stratospheric ozone, IR into tropospheric water vapor and clouds, and Visible into the oceans and biosphere. Succeeding satellite missions being planned for 2006 and 2011 will continue to monitor these critical solar variables.

Cahalan, Robert F.↗

Wind-Tunnel Tests of Several Model Tractor-Propeller and Pusher-Propeller Wing Extension-Shaft Arrangements, Special Report

Tests were made in the 20-foot propeller-research tunnel to investigate the possibility of obtaining increased net efficiencies of propeller-nacelle units by enclosing the engines in the wings and by using extension shafts. A wing of 5-foot chord was fitted with a propeller drive assembly providing for several axial locations of tractor propellers and pusher propellers. A three-blade 4-foot propeller and a three-blade 3 1/2-foot propeller of special design were tested in this wing with spinners and fairings ranging in diameter from 6 to 16 inches. A 16-inch NACA cowling was tested for comparative purposes. Two types of cuffs were also employed. It was found that the net efficiency of a conventional round-shank propeller mounted on an extension shaft in front of or behind a wing increased with an increase in the diameter of the spinner and the shaft housing within the scope of the tests. The largest spinner used had a diameter that might favorably compare with that of a radial engine cowling. The efficiencies for the pusher position appeared to be more critically affected by spinner size than those for the tractor position. The spinners with large diameters for the pusher position resulted in a higher efficiency than those for the corresponding tractor arrangements; the reverse was true for the small spinners. The use of propeller cuffs in combination with a spinner of small diameter generally resulted in net efficiencies that were comparable with those found for the large-spinner combinations.

Harmon, Hubert N.↗

Autonomous Rover Traverse and Precise Arm Placement on Remotely Designated Targets

This software controls a rover platform to traverse rocky terrain autonomously, plan paths, and avoid obstacles using its stereo hazard and navigation cameras. It does so while continuously tracking a target of interest selected from 10 20 m away. The rover drives and tracks the target until it reaches the vicinity of the target. The rover then positions itself to approach the target, deploys its robotic arm, and places the end effector instrument on the designated target to within 2-3-cm accuracy of the originally selected target. This software features continuous navigation in a fairly rocky field in an outdoor environment and the ability to enable the rover to avoid large rocks and traverse over smaller ones. Using point-and-click mouse commands, a scientist designates targets in the initial imagery acquired from the rover s mast cameras. The navigation software uses stereo imaging, traversability analysis, path planning, trajectory generation, and trajectory execution. It also includes visual target tracking of a designated target selected from 10 m away while continuously navigating the rocky terrain. Improvements in this design include steering while driving, which uses continuous curvature paths. There are also several improvements to the traversability analyzer, including improved data fusion of traversability maps that result from pose estimation uncertainties, dealing with boundary effects to enable tighter maneuvers, and handling a wider range of obstacles. This work advances what has been previously developed and integrated on the Mars Exploration Rovers by using algorithms that are capable of traversing more rock-dense terrains, enabling tight, thread-the-needle maneuvers. These algorithms were integrated on the newly refurbished Athena Mars research rover, and were fielded in the JPL Mars Yard. Forty-three runs were conducted with targets at distances ranging from 5 to 15 m, and a success rate of 93% was achieved for placement of the instrument within 2-3 cm of the target.

Nesnas, Issa A.↗

Forests dominate the interannual variability of the North American carbon sink

Understanding what drives the interannual variability (IAV) of the land carbon sink is crucial for improving future predictions of this important, yet uncertain, component of the climate system. While drivers of global and hemispheric-scale net ecosystem exchange (NEE) IAV have been investigated, our understanding of the drivers of NEE IAV at regional scales (e.g. sub-continental, biome-level) is quite poor. Here we explore the biome-level attribution and drivers of North American NEE using inverse estimates derived from a dense network of atmospheric CO2 observations. We find that deciduous broadleaf and mixed forests are the primary regions responsible for North American NEE IAV, which differs from the ecoregions identified for the globe and Northern Hemisphere. We also find that a suite of terrestrial biosphere models (TBMs) do not agree on the dominant biome contributing to NEE IAV, with TBMs falling along an apparent spectrum ranging between those with IAV dominated primarily by forested ecosystems to those with IAV dominated by non-forested ecosystems. Furthermore, this regional trade-off in TBM NEE IAV is found to be linked to differing regional responses to environmental drivers among TBMs. This work displays the importance of extra-tropical forests in driving continental NEE IAV and also highlights the challenges and limitations of using TBMs to inform regional-scale carbon flux dynamics.

54 ENVIRONMENTAL SCIENCES↗

Replica-based Crack Inspection

A surface replica-based crack inspection method has recently been developed for use in Space Shuttle main engine (SSME) hydrogen feedline flowliners. These flowliners exist to ensure favorable flow of liquid hydrogen over gimble joint bellows, and consist of two rings each containing 38 elongated slots. In the summer of 2002, multiple cracks ranging from 0.1 inches to 0.6 inches long were discovered; each orbiter contained at least one cracked flowliner. These long cracks were repaired and eddy current inspections ensured that no cracks longer than 0.075 inches were present. However, subsequent fracture-mechanics review of flight rationale required detection of smaller cracks, and was the driving force for development of higher-resolution inspection method. Acetate tape surface replicas have been used for decades to detect and monitor small cracks. However, acetate tape replicas have primarily been limited to laboratory specimens because complexities involved in making these replicas - requiring acetate tape to be dissolved with acetone - are not well suited for a crack inspection tool. More recently developed silicon-based replicas are better suited for use as a crack detection tool. A commercially available silicon-based replica product has been determined to be acceptable for use in SSME hydrogen feedlines. A method has been developed using this product and a scanning electron microscope for analysis, which can find cracks as small as 0.005 inches and other features (e.g., pits, scratches, tool marks, etc.) as small as 0.001 inches. The resolution of this method has been validated with dozens of cracks generated in a laboratory setting and this method has been used to locate 55 cracks (ranging in size from 0.040 inches to 0.004 inches) on space flight hardware. These cracks were removed by polishing away the cracked material and a second round of replicas confirmed the repair.

Newman, John A.↗

High-speed and High-dynamic Variable Frequency Drive Using Modular Multilevel Converter and SiC Devices

This paper presents a high-speed and high-dynamic variable frequency drive (VFD) based on the modular multilevel converter (MMC) and silicon carbide (SiC) devices. A compact submodule design with the straightforward extension feature is introduced. An integrated control scheme including the low- and high- speed control is developed. Based on the designed prototype and the advanced control scheme, the dynamic response including speed acceleration and torque step-change capabilities are investigated. Besides, the steady-state performance in the highspeed range is also examined in terms of speed control accuracy and current distortion. Validated by the experimental results, the developed VFD prototype with the proposed control scheme can successfully reach the high speed (6000 rpm). It has accurate speed control (±1%) and low current distortion (THD%=2.54%) in high-speed operation, as well as the robust dynamic response

Variable speed, motor drive, high-speed, Modular m↗

Sources of errors in the simulation of south Asian summer monsoon in the CMIP5 GCMs

Accurate simulation of the South Asian summer monsoon (SAM) is still an unresolved challenge. There has not been a benchmark effort to decipher the origin of undesired yet virtually invariable unsuccessfulness of general circulation models (GCMs) over this region. This study analyzes a large ensemble of CMIP5 GCMs to show that most of the simulation errors in the precipitation distribution and their driving mechanisms are systematic and of similar nature across the GCMs, with biases in meridional differential heating playing a critical role in determining the timing of monsoon onset over land, the magnitude of seasonal precipitation distribution and the trajectories of monsoon depressions. Errors in the pre-monsoon heat low over the lower latitudes and atmospheric latent heating over the slopes of Himalayas and Karakoram Range induce significant errors in the atmospheric circulations and meridional differential heating. Lack of timely precipitation further exacerbates such errors by limiting local moisture recycling and latent heating aloft from convection. Most of the summer monsoon errors and their sources are reproducible in the land–atmosphere configuration of a GCM when it is configured at horizontal grid spacing comparable to the CMIP5 GCMs. While an increase in resolution overcomes many modeling challenges, coarse resolution is not necessarily the primary driver in the exhibition of errors over South Asia. These results highlight the importance of previously less well known pre-monsoon mechanisms that critically influence the strength of SAM in the GCMs and highlight the importance of land–atmosphere interactions in the development and maintenance of SAM.

CMIP5↗

SU-E-J-116: Uncertainties Associated with Dose Summation of High-Dose Rate Brachytherapy and Intensity Modulated Radiotherapy for Gynecological Cases

Purpose: Determining the cumulative dose distribution (CDD) for gynecological patients treated with both high-dose rate (HDR) brachytherapy and intensity-modulated radiotherapy (IMRT) is challenging. The purpose of this work is to study the uncertainty of performing this with a structure-guided deformable (SGD) approach in Velocity. Methods: For SGD, the Hounsfield units inside specified contours are overridden to set uniform values. Deformable image registration (DIR) is the run on these process images, which forces the DIR to focus on these contour boundaries. 18 gynecological cancer patients were used in this study. The original bladder and rectum planning contours for these patients were used to drive the SGD. A second set of contours were made of the bladder by the same person with the intent of carefully making them completely consistent with each other. This second set was utilized to evaluate the spatial accuracy of the SGD. The determined spatial accuracy was then multiplied by the local dose gradient to determine a dose uncertainty associated with the SGD dose warping. The normal tissue complication probability (NTCP) was then calculated for each dose volume histogram (DVH) that included four different probabilistic uncertainties associated with the spatial errors (e.g., 68.3% and 95.4%). Results: The NTCPs for each DVH (e.g., NTCP-−95.4%, NTCP-−68.3%, NTCP-68.3%, NTCP-95.4%) differed amongst patients. All patients had an NTCP-−95.4% close to 0%, while NTCP-95.4% ranged from 0.67% to 100%. Nine patients had an NTCP-−95.4% less than 50% while the remaining nine patients had NTCP-95.4% greater than 50%. Conclusion: The uncertainty associated with this CDD technique renders a large NTCP uncertainty. Thus, it is currently not practical for clinical use. The two ways to improve this would be to use more precise contours to drive the SGD and to use a more accurate DIR algorithm.

60 APPLIED LIFE SCIENCES↗

The influence of incubation temperature on offspring traits varies across northern and southern populations of the American alligator ( Alligator mississippiensis )

Maternal provisioning and the developmental environment are fundamental determinants of offspring traits, particularly in oviparous species. However, the extent to which embryonic responses to these factors differ across populations to drive phenotypic variation is not well understood. Here, we examine the contributions of maternal provisioning and incubation temperature to hatchling morphological and metabolic traits across four populations of the American alligator (Alligator mississippiensis), encompassing a large portion of the species' latitudinal range. Our results show that whereas the influence of egg mass is generally consistent across populations, responses to incubation temperature show population-level variation in several traits, including mass, head length, head width, and residual yolk mass. Additionally, the influence of incubation temperature on developmental rate is greater at northern populations, while the allocation of maternal resources toward fat body mass is greater at southern populations. Overall, our results suggest that responses to incubation temperature, relative to maternal provisioning, are a larger source of interpopulation phenotypic variation and may contribute to the local adaptation of populations.

59 BASIC BIOLOGICAL SCIENCES↗

Two-dimensional materials for bio-realistic neuronal computing networks

Two-dimensional (2D) van der Waals materials have found broad utility in a diverse range of applications including electronics, optoelectronics, renewable energy, and quantum information technologies. Meanwhile, exponentially growing digital data coupled with the ubiquity of artificial intelligence algorithms have generated significant interest in edge neuromorphic computing as an alternative to centralized cloud computing. The drive to incorporate neuroscience principles into computing hardware is motivated by the low power consumption, parallel processing, and reconfigurability of the human brain. The diverse library of 2D materials with atomic-level thicknesses, exceptional electrostatic tunability, and integration versatility is particularly well-suited for realizing bio-realistic synaptic and neuronal functionality. Here, we summarize past and present work in this field and outline the frontier challenges that have not yet been overcome. Here we also delineate potential solutions and suggest that the neuroscience principles of criticality and synchrony have the potential to inspire breakthrough applications of 2D materials in neuronal computing networks.

36 MATERIALS SCIENCE↗

Nanoscale-femtosecond dielectric response of Mott insulators captured by two-color near-field ultrafast electron microscopy

Characterizing and controlling the out-of-equilibrium state of nanostructured Mott insulators hold great promises for emerging quantum technologies while providing an exciting playground for investigating fundamental physics of strongly-correlated systems. Here, we use two-color near-field ultrafast electron microscopy to photo-induce the insulator-to-metal transition in a single VO 2 nanowire and probe the ensuing electronic dynamics with combined nanometer-femtosecond resolution (10 –21 m • s). We take advantage of a femtosecond temporal gating of the electron pulse mediated by an infrared laser pulse, and exploit the sensitivity of inelastic electron-light scattering to changes in the material dielectric function. By spatially mapping the near-field dynamics of an individual nanowire of VO 2 , we observe that ultrafast photo-doping drives the system into a metallic state on a timescale of ~150 fs without yet perturbing the crystalline lattice. Due to the high versatility and sensitivity of the electron probe, our method would allow capturing the electronic dynamics of a wide range of nanoscale materials with ultimate spatiotemporal resolution.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

Theory and simulations of linear and nonlinear two-dimensional Rayleigh–Taylor dynamics with variable acceleration

Interfacial Rayleigh–Taylor mixing is crucial to describing important natural and engineering processes, such as exploding supernovae, laser micromachining, hot spots in inertial confinement fusion, and optical telecommunications. These require the characterization of the time dependence of the driving acceleration. We compare our theoretical formulation based on group theory foundations with interface-capturing numerical simulations for linear and nonlinear two-dimensional Rayleigh–Taylor instabilities in a finite-sized domain with time-varying acceleration over broad ranges of Atwood numbers and acceleration exponents. Detailed corroboration between theory and simulations is provided for this foundational case. Both demonstrate the strong interfacial nature of Rayleigh–Taylor instabilities, which suggests that practical flow fields can be reconstructed from the derived fluid potential using the proposed theory. A robust agreement is also obtained for the early and late-time evolution of the amplitudes of the bubble and spike, which demonstrate that the Rayleigh–Taylor flow can transition to the mixing regime even for a single-mode initial perturbation. Corroboration with experiments of high energy density plasmas motivated by studies of supernovae is also achieved. In addition, a long-standing puzzle in Rayleigh–Taylor dynamics on the interplay between the acceleration, the shear, and the interface morphology in the theory and simulations is resolved by accounting for finite viscosity of the fluids. The characterization of Rayleigh–Taylor instabilities as a highly interfacial phenomenon provides valuable insight into its multiscale nature, which enhances the design and understanding of numerous processes of practical interest.

42 ENGINEERING↗

PFPO plasma scenarios for exploration of long pulse operation in ITER

Long Pulse Scenarios (LPS) in ITER foreseen during the Pre-Fusion Power Operation (PFPO) phase of the ITER Research Plan (IRP) are assessed using 1.5D transport simulations within the ASTRA framework. Such assessment is required to predict the operational space for LPS operation in PFPO, as well as to evaluate which physics processes for LPS operation during Fusion Power Operation (FPO) could be studied during PFPO. An important aspect in the development of LPSs in PFPO is to minimize lifetime consumption of the Central Solenoid (CS) for these scenarios. The maximum pulse length achievable for LPSs in PFPO with no consumption of CS lifetime (currents in CS coils $⩽$30 kA per turn) has been assessed for a range of heating schemes and heating mixes, confinement regimes (L-mode and H-mode) and for helium and hydrogen plasmas. The operational space of LPS and pulse length has been explored through density scans with the Heating and Current Drive mix required for the FPO Q $⩾$ 5 steady-state plasma scenario (namely Neutral Beam Injection and Electron Cyclotron Heating) including acceptable shine through losses on the first wall for both helium and hydrogen plasmas. Fast particle physics aspects that are common between FPO plasmas and LPS PFPO H-mode plasmas at low densities are studied including MHD stability analysis with the KINX code and non-perturbative critical gradient model based on high-n Toroidal Alfven Eigenmodes (TAE) stability kinetic ballooning code HINST calculations.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗