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At least 163 records · Page 9

An ancient role for nuclear beta-catenin in the evolution of axial polarity and germ layer segregation

The human oncogene beta-catenin is a bifunctional protein with critical roles in both cell adhesion and transcriptional regulation in the Wnt pathway. Wnt/beta-catenin signalling has been implicated in developmental processes as diverse as elaboration of embryonic polarity, formation of germ layers, neural patterning, spindle orientation and gap junction communication, but the ancestral function of beta-catenin remains unclear. In many animal embryos, activation of beta-catenin signalling occurs in blastomeres that mark the site of gastrulation and endomesoderm formation, raising the possibility that asymmetric activation of beta-catenin signalling specified embryonic polarity and segregated germ layers in the common ancestor of bilaterally symmetrical animals. To test whether nuclear translocation of beta-catenin is involved in axial identity and/or germ layer formation in 'pre-bilaterians', we examined the in vivo distribution, stability and function of beta-catenin protein in embryos of the sea anemone Nematostella vectensis (Cnidaria, Anthozoa). Here we show that N. vectensis beta-catenin is differentially stabilized along the oral-aboral axis, translocated into nuclei in cells at the site of gastrulation and used to specify entoderm, indicating an evolutionarily ancient role for this protein in early pattern formation.

NASA Discipline Evolutionary Biology↗

Tensile adhesion testing methodology for thermally sprayed coatings

The structure of thermally sprayed coatings consists of lamellae which are oriented parallel to the substrate surface. The lamellae separate and fracture by distinctive mechanisms which are reflected in the failure morphology, and these may be described as adhesive (between the coating and substrate), cohesive (within the coating), or mixed mode. There is a large variability in the failure stress for any nominally identical group of coatings. A lower bound for the fracture toughness of alumina coatings can be calculated as 0.2 MNm exp -3/2. The coating strength values may also be treated as belonging to the statistical distribution of the Weibull function. The Weibull modulus of the coating strength varied from 1.4 to 3.8. This analysis infers that the flaw size within coatings is highly variable and that the flaws are nonuniformly dispersed. The present work focuses on the question of whether tensile adhesion tests are an appropriate testing method for thermally sprayed materials.

Berndt, Christopher C.↗

Microcracking in composite laminates under thermal and mechanical loading

Composites used in space structures are exposed to both extremes in temperature and applied mechanical loads. Cracks in the matrix form, changing the laminate thermoelastic properties. The goal of the present investigation is to develop a predictive methodology to quantify microcracking in general composite laminates under both thermal and mechanical loading. This objective is successfully met through a combination of analytical modeling and experimental investigation. In the analysis, the stress and displacement distributions in the vicinity of a crack are determined using a shear lag model. These are incorporated into an energy based cracking criterion to determine the favorability of crack formation. A progressive damage algorithm allows the inclusion of material softening effects and temperature-dependent material properties. The analysis is implemented by a computer code which gives predicted crack density and degraded laminate properties as functions of any thermomechanical load history. Extensive experimentation provides verification of the analysis. AS4/3501-6 graphite/epoxy laminates are manufactured with three different layups to investigate ply thickness and orientation effects. Thermal specimens are cooled to progressively lower temperatures down to -184 C. After conditioning the specimens to each temperature, cracks are counted on their edges using optical microscopy and in their interiors by sanding to incremental depths. Tensile coupons are loaded monotonically to progressively higher loads until failure. Cracks are counted on the coupon edges after each loading. A data fit to all available results provides input parameters for the analysis and shows them to be material properties, independent of geometry and loading. Correlation between experiment and analysis is generally very good under both thermal and mechanical loading, showing the methodology to be a powerful, unified tool. Delayed crack initiation observed in a few cases is attributed to a lack of preexisting flaws assumed by the analysis. Some interactions between adjacent ply groups are attributed to local stress concentrations. These two effects are not captured by the analysis due to its global nature. The analysis is conservative in these cases and agrees well with data after the observed onset of cracking.

Maddocks, Jason R.↗

Interplanetary magnetic field dependency of stable Sun-aligned polar cap arcs

This is the first analysis, using a statistically significant data set, of the morphological dependence of the presence, orientation, and motion of stable sun-aligned polar cap arcs upon the vector interplanetary magnetic field (IMF). For the one winter season analyzed we had 1392 all-sky 630.0-nm images of 2-min resolution containing a total of 150 polar cap arcs, all with corresponding values of the IMF as measured by International Monitoring Platform (IMP) 8 or International Sun Earth Explorer (ISEE) 2. After demonstrating an unbiased data set with smooth normal distributions of events versus the dimensions of time, space, and IMF component, we examine IMF dependencies of the properties of the optical arcs. A well-defined dependence for B(sub z) is found for the presence/absence of stable Sun-aligned polar cap arcs. Consistent with previous statistical studies, the probability of observing polar cap aurora steadily increases for larger positive values of B(sub z), and linearly decreases when B(sub z) becomes more negative. The probability of observing Sun-aligned arcs within the polar cap is determined to vary sharply as a function of the arc location; arcs were observed 40% of the time on the dawnside and only 10% on the duskside. This implies an overall probability of at least 40% for the whole polar cap. 20% of the arcs were observed during 'southward IMF conditions,' but in fact under closer inspection were found to have been formed under northward IMF conditions; these 'residual' positive B(sub z) arcs ha d a delayed residence time in the polar cap of about what would be expected after a north to south transition of B(sub z). A firm dependence on B(sub y) is also found for both the orientation and the dawn-dusk direction of motion of the arcs. All the arcs are Sun-aligned to a first approximation, but present deviations from this orientation, depending primarily upon the location of the arc in corrected geomagnetic (CG) coordinates. The arcs populating the 06-12 and the 12-18 quadrants of the CG coordinate system point toward the cusp. The B(sub y) dependency of the arc alignment is consistent with a cusp displacement in local time according to the sign of B(sub y). We found that the arc direction of motion depended both on B(sub y) and the arc location within the polar cap. For a given value of B(sub y) two well-defined regions (or cells) exist. Within each cell the arcs move in the same direction toward the boundary between the cells. The arcs located in the duskside move dawnward; those in the dawnside move duskward. The relative size of these dusk and dawn regions (or cells) are controlled by the magnitude of B(sub y). This persistent dusk-dawn motion fo the polar cap arcs is interpreted in terms of newly open flux tubes entering the polar cap and exerting a displacement of the convective cells and the polar cap arcs that are embedded within them.

Valladares, C. E.↗

Probabilistic Micromechanics and Macromechanics for Ceramic Matrix Composites

The properties of ceramic matrix composites (CMC's) are known to display a considerable amount of scatter due to variations in fiber/matrix properties, interphase properties, interphase bonding, amount of matrix voids, and many geometry- or fabrication-related parameters, such as ply thickness and ply orientation. This paper summarizes preliminary studies in which formal probabilistic descriptions of the material-behavior- and fabrication-related parameters were incorporated into micromechanics and macromechanics for CMC'S. In this process two existing methodologies, namely CMC micromechanics and macromechanics analysis and a fast probability integration (FPI) technique are synergistically coupled to obtain the probabilistic composite behavior or response. Preliminary results in the form of cumulative probability distributions and information on the probability sensitivities of the response to primitive variables for a unidirectional silicon carbide/reaction-bonded silicon nitride (SiC/RBSN) CMC are presented. The cumulative distribution functions are computed for composite moduli, thermal expansion coefficients, thermal conductivities, and longitudinal tensile strength at room temperature. The variations in the constituent properties that directly affect these composite properties are accounted for via assumed probabilistic distributions. Collectively, the results show that the present technique provides valuable information about the composite properties and sensitivity factors, which is useful to design or test engineers. Furthermore, the present methodology is computationally more efficient than a standard Monte-Carlo simulation technique; and the agreement between the two solutions is excellent, as shown via select examples.

Murthy, Pappu L. N.↗

Magnetic Damping of g-Jitter Driven Flows: 3-D Calculations

A 3-D numerical model is developed to represent the oscillating natural convection induced in a cylindrical cavity filled with Ga-doped germanium with and without the presence of an external magnetic field. The model is developed based on the penalty-finite element solution of the equations describing the transport of momentum, heat and solutal element as well as the electromagnetic field distribution in the melt pool. Automatic time step control is applied to help speed up the calculations. Numerical simulations are conducted to study the convection and magnetic damping effects as a function of frequency, directions and amplitudes of g-jitter and also the direction and magnitudes of the applied magnetic fields. The results show that the g-jitter driven flow is time dependent and exhibits a complex recirculating convection pattern in three dimensions and that an applied magnetic field can be employed to suppress this deleterious convective flow and both magnitude and orientation of the applied field are important in magnetic damping of the g-jitter induced convective flows.

Shang, D. Y.↗

Analysis of Snow Bidirectional Reflectance from ARCTAS Spring-2008 Campaign

The spring 2008 Arctic Research of the Composition of the Troposphere from Aircraft and Satellites (ARCTAS) experiment was one of major intensive field campaigns of the International Polar Year aimed at detailed characterization of atmospheric physical and chemical processes in the Arctic region. A part of this campaign was a unique snow bidirectional reflectance experiment on the NASA P-3B aircraft conducted on 7 and 15 April by the Cloud Absorption Radiometer (CAR) jointly with airborne Ames Airborne Tracking Sunphotometer (AATS) and ground-based Aerosol Robotic Network (AERONET) sunphotometers. The CAR data were atmospherically corrected to derive snow bidirectional reflectance at high 1 degree angular resolution in view zenith and azimuthal angles along with surface albedo. The derived albedo was generally in good agreement with ground albedo measurements collected on 15 April. The CAR snow bidirectional reflectance factor (BRF) was used to study the accuracy of analytical Ross-Thick Li-Sparse (RTLS), Modified Rahman-Pinty-Verstraete (MRPV) and Asymptotic Analytical Radiative Transfer (AART) BRF models. Except for the glint region (azimuthal angles phi less than 40 degrees), the best fit MRPV and RTLS models fit snow BRF to within 0.05. The plane-parallel radiative transfer (PPRT) solution was also analyzed with the models of spheres, spheroids, randomly oriented fractal crystals, and with a synthetic phase function. The latter merged the model of spheroids for the forward scattering angles with the fractal model in the backscattering direction. The PPRT solution with synthetic phase function provided the best fit to measured BRF in the full range of angles. Regardless of the snow grain shape, the PPRT model significantly over-/underestimated snow BRF in the glint/backscattering regions, respectively, which agrees with other studies. To improve agreement with experiment, we introduced a model of macroscopic snow surface roughness by averaging the PPRT solution over the slope distribution function and by adding a simple model of shadows. With macroscopic roughness described by two parameters, the AART model achieved an accuracy of about plus or minus 0.05 with a possible bias of plus or minus 0.03 in the spectral range 0.4-2.2 micrometers. This high accuracy holds at view zenith angles below 55-60 degrees covering the practically important range for remote sensing applications, and includes both glint and backscattering directions.

Lyapustin, A.↗

Semiautonomous Avionics-and-Sensors System for a UAV

Unmanned Aerial Vehicles (UAVs) autonomous or remotely controlled pilotless aircraft have been recently thrust into the spotlight for military applications, for homeland security, and as test beds for research. In addition to these functions, there are many space applications in which lightweight, inexpensive, small UAVS can be used e.g., to determine the chemical composition and other qualities of the atmospheres of remote planets. Moreover, on Earth, such UAVs can be used to obtain information about weather in various regions; in particular, they can be used to analyze wide-band acoustic signals to aid in determining the complex dynamics of movement of hurricanes. The Advanced Sensors and Electronics group at Langley Research Center has developed an inexpensive, small, integrated avionics-and-sensors system to be installed in a UAV that serves two purposes. The first purpose is to provide flight data to an AI (Artificial Intelligence) controller as part of an autonomous flight-control system. The second purpose is to store data from a subsystem of distributed MEMS (microelectromechanical systems) sensors. Examples of these MEMS sensors include humidity, temperature, and acoustic sensors, plus chemical sensors for detecting various vapors and other gases in the environment. The critical sensors used for flight control are a differential- pressure sensor that is part of an apparatus for determining airspeed, an absolute-pressure sensor for determining altitude, three orthogonal accelerometers for determining tilt and acceleration, and three orthogonal angular-rate detectors (gyroscopes). By using these eight sensors, it is possible to determine the orientation, height, speed, and rates of roll, pitch, and yaw of the UAV. This avionics-and-sensors system is shown in the figure. During the last few years, there has been rapid growth and advancement in the technological disciplines of MEMS, of onboard artificial-intelligence systems, and of smaller, faster, and smarter wireless telemetry systems. The major attraction of MEMS lies in orders-of-magnitude reductions of power requirements relative to traditional electronic components that perform equivalent functions. In addition, the compactness of MEMS, relative to functionally equivalent traditional electronics systems, makes MEMS attractive for UAV applications. Recent advances in MEMS have made it possible to produce pressure, acceleration, humidity, and temperature sensors having masses in subgram range and possessing sensitivities and accuracies comparable to those of larger devices.

Shams, Qamar↗

The size distributions of fragments ejected at a given velocity from impact craters

The mass distribution of fragments that are ejected at a given velocity for impact craters is modeled to allow extrapolation of laboratory, field, and numerical results to large scale planetary events. The model is semi-empirical in nature and is derived from: (1) numerical calculations of cratering and the resultant mass versus ejection velocity, (2) observed ejecta blanket particle size distributions, (3) an empirical relationship between maximum ejecta fragment size and crater diameter, (4) measurements and theory of maximum ejecta size versus ejecta velocity, and (5) an assumption on the functional form for the distribution of fragments ejected at a given velocity. This model implies that or planetary impacts into competent rock, the distribution of fragments ejected at a given velocity is broad, e.g., 68% of the mass of the ejecta at a given velocity contains fragments having a mass less than 0.1 times a mass of the largest fragment moving at that velocity. The broad distribution suggests that in impact processes, additional comminution of ejecta occurs after the upward initial shock has passed in the process of the ejecta velocity vector rotating from an initially downward orientation. This additional comminution produces the broader size distribution in impact ejecta as compared to that obtained in simple brittle failure experiments.

Okeefe, J. D.↗

Electron precipitation zones around major ground-based VLF signal sources

The spatial distribution of electron precipitation induced by VLF signals from ground-based transmitters is determined by using a test particle computer model of the gyroresonant wave-particle interaction (Inan et al., 1982). The results are presented as contours of energy flux on a map of the region around each transmitter. It is shown that the size of the precipitation zones is a strong function of the geographic location of the transmitter, as well as its radiated power and operating frequency. In general, the precipitation zones are much wider in longitude than in latitude and are oriented along lines of constant geomagnetic latitude. Assuming backscatter and/or wave echoing, precipitation zones around the points that are magnetically conjugate to the sources are also estimated. The results presented can be used to interpret satellite- or ground-based measurements of the precipitation induced by ground-based VLF transmitters.

Inan, U. S.↗

The size distributions of fragments ejected at a given velocity from impact craters

The mass distribution of fragments that are ejected at a given velocity for impact craters is modeled to allow extrapolation of laboratory, field, and numerical results to large scale planetary events. The model is semi-empirical in nature and is derived from: (1) numerical calculations of cratering and the resultant mass versus ejection velocity, (2) observed ejecta blanket particle size distributions, (3) an empirical relationship between maximum ejecta fragment size and crater diameter, (4) measurements and theory of maximum ejecta size versus ejecta velocity, and (5) an assumption on the functional form for the distribution of fragments ejected at a given velocity. This model implies that for planetary impacts into competent rock, the distribution of fragments ejected at a given velocity is broad, e.g., 68 percent of the mass of the ejecta at a given velocity contains fragments having a mass less than 0.1 times a mass of the largest fragment moving at that velocity. The broad distribution suggests that in impact processes, additional comminution of ejecta occurs after the upward initial shock has passed in the process of the ejecta velocity vector rotating from an initially downward orientation. This additional comminution produces the broader size distribution in impact ejecta as compared to that obtained in simple brittle failure experiments.

O'Keefe, John D.↗

Current Limitations on VLBI Accuracy

The contribution of VLBI to geophysics and geodesy arises from its ability to measure distances between stations in a network and to determine the orientation of stations in the network as well as the orientation of the network with respect to the external reference frame of extragalactic radio objects. Integrating nearly two decades of observations provides useful information about station positions and velocities and the orientation of the Earth, but the complications of the real world and the limitations of observing, modeling and analysis prevent recovery of all effects. Of the factors that limit the accuracy of seemingly straightforward geodetic parameters, the neutral propagation medium has been subject to the greatest scrutiny, but the treatment of the mapping function, the wet component and spatial/temporal inhomogeneities is still improving. These affect both the terrestrial scale and consistency over time. The modeling of non-secular site motions (tides and loading) has increased in sophistication, but there are some differences between the models and the observations. VLBI antennas are massive objects, so their behavior is quite unlike GPS monuments, but antenna deformations add some (generally) unmodeled signal. Radio sources used in geodetic VLBI observations are selected for strength and (relative) absence of structure, but apparent changes in position can leak into geodetic parameters. A linear rate of change of baseline or site parameters is the simplest model and its error improves with time span. However, in most cases the VLBI data distribution is insufficient to look for real non-linear behavior that might affect the average rate. A few sites have multiple VLBI antennas, and some show small differences in rate. VLBI intrinsically measures relative positions and velocities, but individual site positions and velocities are generally more useful. The creation of the VLBI terrestrial reference frame, which transforms relative information into individual results, is an empirical process that has intrinsic errors. While UT1 is uniquely measured by VLBI, the geographical distribution and availability of VLBI stations, especially in the southern hemisphere, and the consistency of the VLBI terrestrial reference frame may limit the accuracy of Earth orientation measurements. The effects of particular error sources on geodetic and geophysical parameters derived from VLBI data will be illustrated.

Ma, Chopo↗

Effects of artificially produced defects on film thickness distribution in sliding EHD point contacts

The effects of artificially produced dents and grooves on the elastohydrodynamic (EHD) film thickness profile in a sliding point contact were investigated by means of optical interferometry. The defects, formed on the surface of a highly polished ball, were held stationary at various locations within and in the vicinity of the contact region while the disk was rotating. It is shown that the defects, having a geometry similar to what can be expected in practice, can dramatically change the film thickness which exists when no defects are present in or near the contact. This change in film thickness is mainly a function of the position of the defects in the inlet region, the geometry of the defects, the orientation of the defects in the case of grooves, and the depth of the defect relative to the central film thickness.

Cusano, C.↗

Real-Time Payload Control and Monitoring on the World Wide Web

World Wide Web (W3) technologies such as the Hypertext Transfer Protocol (HTTP) and the Java object-oriented programming environment offer a powerful, yet relatively inexpensive, framework for distributed application software development. This paper describes the design of a real-time payload control and monitoring system that was developed with W3 technologies at NASA Ames Research Center. Based on Java Development Toolkit (JDK) 1.1, the system uses an event-driven "publish and subscribe" approach to inter-process communication and graphical user-interface construction. A C Language Integrated Production System (CLIPS) compatible inference engine provides the back-end intelligent data processing capability, while Oracle Relational Database Management System (RDBMS) provides the data management function. Preliminary evaluation shows acceptable performance for some classes of payloads, with Java's portability and multimedia support identified as the most significant benefit.

Sun, Charles↗

General equilibrium characteristics of a dual-lift helicopter system

The equilibrium characteristics of a dual-lift helicopter system are examined. The system consists of the cargo attached by cables to the endpoints of a spreader bar which is suspended by cables below two helicopters. Results are given for the orientation angles of the suspension system and its internal forces, and for the helicopter thrust vector requirements under general circumstances, including nonidentical helicopters, any accelerating or static equilibrium reference flight condition, any system heading relative to the flight direction, and any distribution of the load to the two helicopters. Optimum tether angles which minimize the sum of the required thrust magnitudes are also determined. The analysis does not consider the attitude degrees of freedom of the load and helicopters in detail, but assumes that these bodies are stable, and that their aerodynamic forces in equilibrium flight can be determined independently as functions of the reference trajectory. The ranges of these forces for sample helicopters and loads are examined and their effects on the equilibrium characteristics are given parametrically in the results.

Cicolani, L. S.↗

A stochastic bioburden model for spacecraft sterilization.

Development of a stochastic model of the probability distribution for the random variable representing the number of microorganisms on a surface as a function of time. The first basic principle associated with bioburden estimation is that viable particles are removed from surfaces. The second notion important to the analysis is that microorganisms in environments and on surfaces occur in clumps. The last basic principle relating to bioburden modeling is that viable particles are deposited on a surface. The bioburden on a spacecraft is determined by the amount and kind of control exercised on the spacecraft assembly location, the shedding characteristics of the individuals in the vicinity of the spacecraft, its orientation, the geographical location in which the assembly takes place, and the steps in the assembly procedure. The model presented has many of the features which are desirable for its use in the spacecraft sterilization programs currently being planned by NASA.

Roark, A. L.↗

The induced magnetosphere of the moon. 1: Theory

An analytic solution for the magnetic field in the space defined by a spherical moon and its downstream cylindrical cavity formed by the solar wind is derived for interplanetary magnetic fields both parallel and perpendicular to the cavity axis. By superposition, the solution is obtained for arbitrary orientations of the interplanetary field. The theory is quasi-static and is formulated in terms of a scalar magnetic potential. Thus the moon model consists of a core of arbitrary size and infinite electrical conductivity surrounded by a nonconducting shell; the cavity volume is assumed to be nonconducting. The variation of the magnetic field on the lunar surface, both on the sunlit and on the dark side hemispheres, and on the cavity boundary is presented for various values of core radius. The solution yields the distribution of currents on the lunar sunlit surface and the surface of the cavity. Theoretical transfer functions are presented and their variations with position on the lunar surface and with core size are discussed.

Schubert, G.↗

CFD Predictions of Boiling Regime Transitions during Line Chilldown validated against a 1G LN2 Experiment

Introduction Before filling a propellant tank on the ground or in Space, the transfer line between the donor and receiver tanks must be cooled down preferably by sacrificing a minimum amount of the cryogenic fluid. The cryogenic line chill-down process involves a transition between different flow boiling regimes, namely, film boiling, transition film boiling, and nucleate boiling which are complex and may be quite gravity-dependent. Capturing these boiling phenomena and predicting the transition between them in a CFD framework is new and challenging both for 1g and microgravity applications. Materials & Methods The present work addresses this challenge by employing a two-phase Eulerian approach in the context of a homogeneous fluid mixture together with the Lee phase change model to capture the film boiling regime of the chill-down process using ANSYS Fluent®. The nucleate boiling regime is predicted by incorporating an in-house developed sub-grid model that accounts for bubble nucleation, bubble growth, bubble departure diameter, and their shedding frequency. The sub-grid model is encoded and implemented into Fluent via a user-defined function for the wall-fluid heat flux calculations. The mathematical formulation and numerical implementation of the CFD model are described in detail. The coupled CFD-Subgrid model is validated against published experimental data for liquid nitrogen chill-down of a heated stainless-steel pipe in 1g. Results Numerical simulation results show good agreements between the CFD predictions of the wall temperature evolution, rewetting temperature, and transition between film and nucleate boiling, with the experimental measurements published by Darr et al [2] for several different LN2 flowrates in the vertical pipe orientation. The CFD predictions for the wall temperature distribution indicate a rapid quenching of the wall at two upstream and downstream temperature sensing locations as compared to the experimental measurement. The only tuning parameter in the CFD model is the Lee mass transfer coefficient. The CFD Model predicts the Liedenfrost rewetting temperature in close agreement with the experiment. This marks a transition between stable and transitionary flow boiling regimes. The CFD-predicted boiling curve for the downstream sensor location is also compared against its experimental counterpart and indicates that the model is able to predict all the key temperature and heat flux parameters during the transitions from stable to transitionary film boiling to nucleate boiling in close agreement with the experiment. A sequence of predicted volume fraction, and temperature contours depicting these transitions will be presented.

Evaporation Condensation↗