High-performance electrochemical immunomagnetic assay for breast cancer analysis
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Freitas, Maria↗
SEARCH · Engineering Papers
Results for “assays”
Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
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Chemical effects on neural network activity: Comparison of acute versus network formation exposure in microelectrode array assays
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Pharmacology & Pharmacy↗
The impact of comet assay data normalization in human biomonitoring studies outcomes
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Esteves, Filipa↗
Analysis of secondary pharmacology assays received by the US Food and Drug Administration
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60 APPLIED LIFE SCIENCES↗
The CAM assay in the study of the metastatic process
Among the in vivo experimental models, the chick embryo chorioallantoic membrane (CAM) has been routinely used to implant several malignant cell lines or tumor tissues to study their angiogenic and metastatic capability. Since the chick embryo is naturally immunodeficient, the CAM can support the engraftment of tumor cells, and their growth therein can faithfully recapitulate most of the characteristics of the carcinogenic process including: growth, invasion, angiogenesis and colonization of distant tissues. This review article is focused on the discussion of the more recent literature data concerning the use of the CAM to investigate the metastatic process.
60 APPLIED LIFE SCIENCES↗
Assessing utility of thyroid in vitro screening assays through comparisons to observed impacts in vivo
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Eytcheson, Stephanie A. (ORCID:0000000200072421)↗
Fieldable Microfluidic Platform for Separation and Assay of U and Pu from Fission Samples in Environmental Matrices
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and nuclear chemistry↗
Cross-Reactivity of Chili Peppers ( Capsicum sp.) with the xMAP Food Allergen Detection Assay
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Agriculture↗
Assessing the Perturbing Effects of Drugs on Lipid Bilayers Using Gramicidin Channel-Based In Silico and In Vitro Assays
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59 BASIC BIOLOGICAL SCIENCES↗
Biolayer Interferometry Assay for Cyclin-Dependent Kinase-Cyclin Association Reveals Diverse Effects of Cdk2 Inhibitors on Cyclin Binding Kinetics
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Biochemistry & Molecular Biology↗
A Fluorescence Polarization Assay for Macrodomains Facilitates the Identification of Potent Inhibitors of the SARS-CoV-2 Macrodomain
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Biochemistry & Molecular Biology↗
Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization
Successes in biocatalytic polyester recycling have raised the possibility of deconstructing alternative polymers enzymatically, with polyamide (PA) being a logical target due to the array of amide-cleaving enzymes present in nature. Here, we screen 40 potential natural and engineered nylon-hydrolyzing enzymes (nylonases), using mass spectrometry to quantify eight compounds resulting from enzymatic nylon-6 (PA6) hydrolysis. Comparative time-course reactions incubated at 40-70 °C showcase enzyme-dependent variations in product distributions and extent of PA6 film depolymerization, with significant nylon deconstruction activity appearing rare. The most active nylonase, a NylC K variant we rationally thermostabilized (an N-terminal nucleophile (Ntn) hydrolase, NylC K -TS, T m = 87.4 °C, 16.4 °C higher than the wild-type), hydrolyzes 0.67 wt% of a PA6 film. Reactions fail to restart after fresh enzyme addition, indicating that substrate-based limitations, such as restricted enzyme access to hydrolysable bonds, prohibit more extensive deconstruction. Overall, this study expands our understanding of nylonase activity distribution, indicates that Ntn hydrolases may have the greatest potential for further development, and identifies key targets for progressing PA6 enzymatic depolymerization, including improving enzyme activity, product selectivity, and enhancing polymer accessibility.
09 BIOMASS FUELS↗