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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 163 records · Page 9

Imaging Intact Filamentous Fungi from Spore to Hyphal Tip on the Nanoscale

Previously, the Pacific Northwest National Laboratory was limited by a 3-order of magnitude gap in resolution for direct 2-D and 3-D imaging of chemical, biological, energy and materials science systems. Our imaging instrumentation could directly visualize 3-D ultrastructure without labeling at resolutions less than 10-nanometers or greater than 10-micrometers. To address this gap, we procured and commissioned a Sigray NanoFast Bio-Lambda laboratory-based x-ray nanotomography system. This equipment provides the capability to image through tens of micrometers of organic materials at down to 35-nanometer resolution to bridge the nanoscale to the mesoscale for biological systems, organic polymers, environmental and particulate samples.

47 OTHER INSTRUMENTATION↗

NSUF Report EBSD analyses on metallic fuel samples: Tips and improvements in sample preparation

EBSD (Electron Backscatter Diffraction) is an analytic technique used to evaluate the microstructure of a specimen. It can be used to evaluate interesting crystallographic data ranging from grains size to grain orientation, from grain boundaries to stresses. Moreover, in the field of nuclear materials EBSD can aid in estimating properties important for reactor performance, such as dislocation and residual strain [1]. EBSD patterns are generated by focusing a stationary electron gun on the specimen and detecting the backscatter electrons that satisfy Bragg conditions, and thus are diffracted by the crystal planes forming the so called Kikuchi bands. These bands correspond to each of the lattice diffracting crystal planes and are detected on a phosphor screen via a low-light CCD camera [2]. The patterns are generated within a small interaction volume located at a depth of less than 50-100 nm. Because of this, the data quality is extremely sensitive to the integrity of the crystallographic lattice order at the surface of the sample and thus to sample preparation and to oxidation [3]. While conventional sample preparation methods are widely used in materials science, they often do not lead to quality electron backscatter patterns needed for the characterization of nuclear materials [4]. This has been related by many authors to the challenges associated with handling radiological specimens, their rapid oxidation, and artifacts in such samples (e.g. high number of defects, precipitates and/or porosities) [4-8]. EBSD, however, can be an invaluable technique to evaluate microstructural evolution during fabrication and after irradiation at the required mesoscale for model and simulation. It also provides important information on grain structure necessary to understand irradiation effect, such as grain subdivision. Thus, it is of paramount importance to further the application of this technique to nuclear materials and especially irradiated fuels.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Venado acceptance: results and tips [Slides]

Nvidia compiler support is not available through cray-mpich/compiler wrapper interface. Adjust CMAKE files to use the correct COMPILER_ID in conditionals and explicit variables to package flags. Use pinned host memory in cray-libsci_acc and cublasXt calls. Set a large blockDim for cublasXt calls. Try MPS and/or explicit numactl binding if performance is lackluster. Use CRAY_MALLOPT_OFF=1 if unexpected OOM errors appear using cce. Use MPICH_SMP_SINGLE_COPY_MODE=CMA for xpmem issues. Use MPICH_OPT_THREAD_SYNC=0 for MPI_THREAD issues. Poor CUDA-aware MPI performance remains an issue.

97 MATHEMATICS AND COMPUTING↗

Open-source Tip-and-Cue for Change-Detection Tasking

Currently, no tool exists to detect daily change across the globe, let alone to do so cost- and resource-efficiently. The proposed tool will use open-source data to detect change in near-real-time to identify targets of interest for tasking, providing a unique capability for constant global situational awareness.

97 MATHEMATICS AND COMPUTING↗

Global Archaeal Diversity Revealed Through Massive Data Integration: Uncovering Just Tip of Iceberg

The domain of Archaea has gathered significant interest for its ecological and biotechnological potential and its role in helping us to understand the evolutionary history of Eukaryotes. In comparison to the bacterial domain, the number of adequately described members in Archaea is relatively low, with less than 1000 species described. It is not clear whether this is solely due to the cultivation difficulty of its members or, indeed, the domain is characterized by evolutionary constraints that keep the number of species relatively low. Based on molecular evidence that bypasses the difficulties of formal cultivation and characterization, several novel clades have been proposed, enabling insights into their metabolism and physiology. Given the extent of global sampling and sequencing efforts, it is now possible and meaningful to question the magnitude of global archaeal diversity based on molecular evidence. To do so, we extracted all sequences classified as Archaea from 500 thousand amplicon samples available in public repositories. After processing through our highly conservative pipeline, we named this comprehensive resource the ‘Global Archaea Diversity’ (GAD), which encompassed nearly 3 million molecular species clusters at 97% similarity, and organized it into over 500 thousand genera and nearly 100 thousand families. Saline environments have contributed the most to the novel taxa of this previously unseen diversity. The majority of those 16S rRNA gene sequence fragments were verified by matches in metagenomic datasets from IMG/M. These findings reveal a vast and previously overlooked diversity within the Archaea, offering insights into their ecological roles and evolutionary importance while establishing a foundation for the future study and characterization of this intriguing domain of life.

59 BASIC BIOLOGICAL SCIENCES↗

The Near-infrared Spectrum of the Nuclear Star Cluster: Looking below the Tip of the Iceberg, and Comparisons with Extragalactic Nuclei

Long-slit near-infrared (NIR) spectra of the Galactic nuclear star cluster (NSC) are discussed. The spectra sample the major axis of the NSC out to its half-light radius. The absorption spectrum of the central regions of the NSC is averaged over angular scales of tens of arc seconds in order to sample globular cluster-like total luminosities, and the results are compared with model spectra. The equivalent widths of Na i2.21 μm and Ca 2.26 μm outside of the center of the NSC, where light from nuclear bulge stars contributes a large fraction to the total flux, are consistent with solar chemical mixtures. In contrast, the equivalent widths of Na i2.21 μm and Ca i2.26 μm near the center of the NSC are larger than expected from models with solar chemical mixtures, even after light from the brightest evolved stars is removed. The depths of spectroscopic features changing along the major axis of the NSC are consistent with imaging studies that have found evidence of population gradients in the NSC. That Na i2.21 μm and Ca i2.26 μm are deeper than predicted for solar chemical mixtures over a range of evolutionary states is consistent with previous studies that find that the majority of stars near the center of the NSC formed from material that had nonsolar chemical mixtures. The depths of the Na i2.21 μm and Ca i2.26 μm features in the central regions of the NSC are comparable to those in the nuclear spectrum of the early-type Virgo disk galaxy NGC 4491, and are deeper than in the central spectra of NGC 253 and 7793. A spectrum of nebular emission and the youngest stars near the galactic center is also extracted. The equivalent widths of emission features in the extracted NIR spectrum are similar to those in the nuclear spectrum of NGC 253, and it is argued that this agreement is best achieved if the current episode of star formation near the center of the NSC has been in progress for at least a few megayears.

79 ASTRONOMY AND ASTROPHYSICS↗