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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 163 records · Page 9
All sourcesNASA NTRSDOE OSTI
NASA NTRS1984

Gravitropism in leafy dicot stems

In an attempt to separate plant responses to mechanical stresses from responses to gravity compensation, six treatments were automated: (1) upright stationary controls; (2) horizontal clinostat; (3) intermittent clinostat (plants upright 3.3 minutes out of every 4 minutes, horizontal and rotated once in the remaining time); (4) inversion every ten minutes (plants upside down half the time); (5) inversion and immediate return to the vertical; and (6) vertical rotation. Epinasty appeared only on clinostated and on inverted plants, both subjected to gravity compensation. The mechanics of gravitropic stem bending and the effects of a unilateral application of ethephon of gravitropic bending were also investigated.

Salisbury, F. B.↗
NASA NTRS1991

Soybean stem growth under high-pressure sodium with supplemental blue lighting

To study high-pressure sodium (HPS) lamps used for plant lighting because of their high energy conversion efficiencies, 'McCall' soybean plants were grown for 28 days in growth chambers utilizing HPS lamps, with/without supplemental light from blue phosphor fluorescent lamps. Total photosynthetic photon flux levels, including blue fluorescent, were maintained near 300 or 500 micromol/sq m s. Results indicate that employment of HPS or other blue-deficient sources for lighting at low to moderate photosynthetic photon flux levels may cause abnormal stem elongation, but this can be prevented by the addition of a small amount of supplemental blue light.

Wheeler, R. M.↗
NASA NTRS2000

BOREAS RSS-17 Stem, Soil, and Air Temperature Data

The BOREAS RSS-17 team collected several data sets in support of its research in monitoring and analyzing environmental and phenological states using radar data. This data set consists of tree bole and soil temperature measurements from various BOREAS flux tower sites. Temperatures were measured with thermistors implanted in the hydroconductive tissue of the trunks of several trees at each site and at various depths in the soil. Data were stored on a data logger at intervals of either 1 or 2 hours. The majority of the data were acquired between early 1994 and early 1995. The primary product of this data set is the diurnal stem temperature measurements acquired for selected trees at five BOREAS tower sites. The data are provided in tabular ASCII format. The data files are available on a CD-ROM (see document number 20010000884) or from the Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC).

Zimmerman, Reiner↗
NASA NTRS2000

BOREAS TE-2 Stem Growth and Sapwood Data

The BOREAS TE-2 team collected several data sets in support of its efforts to characterize and interpret information on the respiration of the foliage, roots, and wood of boreal vegetation. This data set contains measurements of growth and sapwood of the stems conducted in the NSA during the growing season of 1994. The data are available in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884), or from the Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC).

Hall, Forrest G.↗
NASA NTRS2001

Simple Thermal Environment Model (STEM) User's Guide

This report presents a Simple Thermal Environment Model (STEM) for determining appropriate engineering design values to specify the thermal environment of Earth-orbiting satellites. The thermal environment of a satellite, consists of three components: (1) direct solar radiation, (2) Earth-atmosphere reflected shortwave radiation, as characterized by Earth's albedo, and (3) Earth-atmosphere-emitted outgoing longwave radiation (OLR). This report, together with a companion "guidelines" report provides methodology and guidelines for selecting "design points" for thermal environment parameters for satellites and spacecraft systems. The methods and models reported here are outgrowths of Earth Radiation Budget Experiment (ERBE) satellite data analysis and thermal environment specifications discussed by Anderson and Smith (1994). In large part, this report is intended to update (and supersede) those results.

Justus, C.G.↗
NASA NTRS2003

The Hematopoietic Stem Cell Therapy for Exploration of Deep Space

It is hypothesized that the hematopoietic stem cell therapy (HSCT) might countermeasure various space-caused disorders so as to maintain astronauts' homeostasis. If this were achievable, the HSCT could promote human exploration of deep space. Using animal models of disorders (hindlimb suspension unloading system and beta-thalassemia), the HSCT was tested for muscle loss, immunodeficiency and space anemia. The results indicate feasibility of HSCT for these disorders. To facilitate the HSCT in space, growth of HSCs were optimized in the NASA Rotating Wall Vessel (RWV) culture systems, including Hydrodynamic Focusing Bioreactor (HFB).

Ohi, Seigo↗
NASA NTRS1987

Shoot inversion inhibition of stem elongation in Pharbitis nil: a possible role for ethylene-induced glycoprotein and lignin

Inversion of the upper shoot of Pharbitis nil results in the inhibition of elongation in the inverted stem. The objective of the present study was to determine how shoot inversion-induced gravity stress inhibited elongation and to elucidate the possible role of ethylene-induced glycoprotein and lignin in this process. Determinations of hydroxyproline, peroxidase, phenylalanine ammonia-lyase (PAL), phenol, and lignin content/activity were carried out by appropriate spectrophotometric methods. It was found that inversion and Ethrel treatments of upright shoots caused significant increases in hydroxyproline content, peroxidase, and PAL activity in 12 hours and in phenol and lignin contents in 24 hours. All of these increases except for that of cytoplasmic peroxidase activity were partially reversed by AgNO3, the ethylene action inhibitor. It is concluded that possible cross-linking associated with the accumulation of the ethylene-induced hydroxyproline-rich glycoprotein and lignin may be responsible for the later stages of cessation of elongation in the inverted Pharbitis shoot.

NASA Program Space Biology↗
NASA NTRS1992

Genetic separation of phototropism and blue light inhibition of stem elongation

Blue light-induced regulation of cell elongation is a component of the signal response pathway for both phototropic curvature and inhibition of stem elongation in higher plants. To determine if blue light regulates cell elongation in these responses through shared or discrete pathways, phototropism and hypocotyl elongation were investigated in several blue light response mutants in Arabidopsis thaliana. Specifically, the blu mutants that lack blue light-dependent inhibition of hypocotyl elongation were found to exhibit a normal phototropic response. In contrast, a phototropic null mutant (JK218) and a mutant that has a 20- to 30-fold shift in the fluence dependence for first positive phototropism (JK224) showed normal inhibition of hypocotyl elongation in blue light. F1 progeny of crosses between the blu mutants and JK218 showed normal phototropism and inhibition of hypocotyl elongation, and approximately 1 in 16 F2 progeny were double mutants lacking both responses. Thus, blue light-dependent inhibition of hypocotyl elongation and phototropism operate through at least some genetically distinct components.

Non-NASA Center↗
NASA NTRS1991

Photoinhibition of stem elongation by blue and red light: effects on hydraulic and cell wall properties

The underlying mechanism of photoinhibition of stem elongation by blue (BL) and red light (RL) was studied in etiolated seedlings of pea (Pisum sativum L. cv Alaska). Brief BL irradiations resulted in fast transient inhibition of elongation, while a delayed (lag approximately 60 minutes) but prolonged inhibition was observed after brief RL. Possible changes in the hydraulic and wall properties of the growing cells during photoinhibition were examined. Cell sap osmotic pressure was unaffected by BL and RL, but both irradiations increased turgor pressure by approximately 0.05 megapascal (pressure-probe technique). Cell wall yielding was analyzed by in vivo stress relaxation (pressure-block technique). BL and RL reduced the initial rate of relaxation by 38 and 54%, while the final amount of relaxation was decreased by 48 and 10%, respectively. These results indicate that RL inhibits elongation mainly by lowering the wall yield coefficient, while most of the inhibitory effect of BL was due to an increase of the yield threshold. Mechanical extensibility of cell walls (Instron technique) was decreased by BL and RL, mainly due to a reduction in the plastic component of extensibility. Thus, photoinhibitions of elongation by both BL and RL are achieved through changes in cell wall properties, and are not due to effects on the hydraulic properties of the cell.

NASA Program Space Biology↗
NASA NTRS1990

Coupling of solute transport and cell expansion in pea stems

As cells expand and are displaced through the elongation zone of the epicotyl of etiolated pea (Pisum sativum L. var Alaska) seedlings, there is little net dilution of the cell sap, implying a coordination between cell expansion and solute uptake from the phloem. Using [14C] sucrose as a phloem tracer (applied to the hypogeous cotyledons), the pattern of label accumulation along the stem closely matched the growth rate pattern: high accumulation in the growing zone, little accumulation in nongrowing regions. Several results suggest that a major portion of phloem contents enters elongating cells through the symplast. We propose that the coordination between phloem transport and cell expansion is accomplished via regulatory pathways affecting both plasmodesmata conductivity and cell expansion.

NASA Discipline Plant Biology↗
NASA NTRS1999

Sequential and coordinated action of phytochromes A and B during Arabidopsis stem growth revealed by kinetic analysis

Photoreceptor proteins of the phytochrome family mediate light-induced inhibition of stem (hypocotyl) elongation during the development of photoautotrophy in seedlings. Analyses of overt mutant phenotypes have established the importance of phytochromes A and B (phyA and phyB) in this developmental process, but kinetic information that would augment emerging molecular models of phytochrome signal transduction is absent. We have addressed this deficiency by genetically dissecting phytochrome-response kinetics, after having solved the technical issues that previously limited growth studies of small Arabidopsis seedlings. We show here, with resolution on the order of minutes, that phyA initiated hypocotyl growth inhibition upon the onset of continuous red light. This primary contribution of phyA began to decrease after 3 hr of irradiation, the same time at which immunochemically detectable phyA disappeared and an exclusively phyB-dependent phase of inhibition began. The sequential and coordinated actions of phyA and phyB in red light were not observed in far-red light, which inhibited growth persistently through an exclusively phyA-mediated pathway.

NASA Discipline Plant Biology↗
NASA NTRS1998

Modeling learning in brain stem and cerebellar sites responsible for VOR plasticity

A simple model of vestibuloocular reflex (VOR) function was used to analyze several hypotheses currently held concerning the characteristics of VOR plasticity. The network included a direct vestibular pathway and an indirect path via the cerebellum. An optimization analysis of this model suggests that regulation of brain stem sites is critical for the proper modification of VOR gain. A more physiologically plausible learning rule was also applied to this network. Analysis of these simulation results suggests that the preferred error correction signal controlling gain modification of the VOR is the direct output of the accessory optic system (AOS) to the vestibular nuclei vs. a signal relayed through the cerebellum via floccular Purkinje cells. The potential anatomical and physiological basis for this conclusion is discussed, in relation to our current understanding of the latency of the adapted VOR response.

NASA Discipline Neuroscience↗
NASA NTRS1994

Conventional and cross-correlation brain-stem auditory evoked responses in the white leghorn chick: rate manipulations

Rate-dependent changes in the chick brain-stem auditory evoked response (BAER) using conventional averaging and a cross-correlation technique were investigated. Five 15- to 19-day-old white leghorn chicks were anesthetized with Chloropent. In each chick, the left ear was acoustically stimulated. Electrical pulses of 0.1-ms duration were shaped, attenuated, and passed through a current driver to an Etymotic ER-2 which was sealed in the ear canal. Electrical activity from stainless-steel electrodes was amplified, filtered (300-3000 Hz) and digitized at 20 kHz. Click levels included 70 and 90 dB peSPL. In each animal, conventional BAERs were obtained at rates ranging from 5 to 90 Hz. BAERs were also obtained using a cross-correlation technique involving pseudorandom pulse sequences called maximum length sequences (MLSs). The minimum time between pulses, called the minimum pulse interval (MPI), ranged from 0.5 to 6 ms. Two BAERs were obtained for each condition. Dependent variables included the latency and amplitude of the cochlear microphonic (CM), wave 2 and wave 3. BAERs were observed in all chicks, for all level by rate combinations for both conventional and MLS BAERs. There was no effect of click level or rate on the latency of the CM. The latency of waves 2 and 3 increased with decreasing click level and increasing rate. CM amplitude decreased with decreasing click level, but was not influenced by click rate for the 70 dB peSPL condition. For the 90 dB peSPL click, CM amplitude was uninfluenced by click rate for conventional averaging. For MLS BAERs, CM amplitude was similar to conventional averaging for longer MPIs.(ABSTRACT TRUNCATED AT 250 WORDS).

NASA Discipline Neuroscience↗
NASA NTRS2004

Red light regulation of ethylene biosynthesis and gravitropism in etiolated pea stems

During gravitropism, the accumulation of auxin in the lower side of the stem causes increased growth and the subsequent curvature, while the gaseous hormone ethylene plays a modulating role in regulating the kinetics of growth asymmetries. Light also contributes to the control of gravitropic curvature, potentially through its interaction with ethylene biosynthesis. In this study, red-light pulse treatment of etiolated pea epicotyls was evaluated for its effect on ethylene biosynthesis during gravitropic curvature. Ethylene biosynthesis analysis included measurements of ethylene; the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC); malonyl-conjugated ACC (MACC); and expression levels of pea ACC oxidase (Ps-ACO1) and ACC synthase (Ps-ACS1, Ps-ACS2) genes by reverse transcriptase-polymerase chain reaction analysis. Red-pulsed seedlings were given a 6 min pulse of 11 micromoles m-2 s-1 red-light 15 h prior to horizontal reorientation for consistency with the timeline of red-light inhibition of ethylene production. Red-pulse treatment significantly reduced ethylene production and MACC levels in epicotyl tissue. However, there was no effect of red-pulse treatment on ACC level, or expression of ACS or ACO genes. During gravitropic curvature, ethylene production increased from 60 to 120 min after horizontal placement in both control and red-pulsed epicotyls. In red-pulsed tissues, ACC levels increased by 120 min after horizontal reorientation, accompanied by decreased MACC levels in the lower portion of the epicotyl. Overall, our results demonstrate that ethylene production in etiolated epicotyls increases after the initiation of curvature. This ethylene increase may inhibit cell growth in the lower portion of the epicotyl and contribute to tip straightening and reduced overall curvature observed after the initial 60 min of curvature in etiolated pea epicotyls.

Non-NASA Center↗
NASA NTRS2004

Hubble Space Telescope Bi-Stem Thermal Shield Analyses

The Hubble Space Telescope (HST) was launched April 24, 1990, and was deployed April 25 into low Earth orbit (LEO). It was soon discovered that the metal poles holding the solar arrays were expanding and contracting as the telescope orbited the Earth passing between the sunlight and the Earth s shadow. The expansion and contraction, although very small, was enough to cause the telescope to shake because of thermal-induced jitters, a detrimental effect when trying to take pictures millions of miles away. Therefore, the European Space Agency (ESA, the provider of the solar arrays) built new solar arrays (SA-11) that contained bi-stem thermal shields which insulated the solar array metal poles. These thermal shields were made of 2 mil thick aluminized-Teflon fluorinated ethylene propylene (FEP) rings fused together into a circular bellows shape. The new solar arrays were put on the HST during an extravehicular activity (EVA), also called an astronaut space walk, during the first servicing mission (SM1) in December 1993. An on-orbit photograph of the HST with the SA-11, and a close up of the bellows-like structure of the thermal shields is provided in Figure 1.

Finlay, Katherine A.↗
NASA NTRS2004

Modeled Microgravity Disrupts Collagen I/Integrin Signaling During Osteoblastic Differentiation of Human Mesenchymal Stem Cells

Spaceflight leads to reduced bone mineral density in weight bearing bones that is primarily attributed to a reduction in bone formation. We have previously demonstrated severely reduced osteoblastogenesis of human mesenchymal stem cells (hMSC) following seven days culture in modeled microgravity. One potential mechanism for reduced osteoblastic differentiation is disruption of type I collagen-integrin interactions and reduced integrin signaling. Integrins are heterodimeric transmembrane receptors that bind extracellular matrix proteins and produce signals essential for proper cellular function, survival, and differentiation. Therefore, we investigated the effects of modeled microgravity on integrin expression and function in hMSC. We demonstrate that seven days of culture in modeled microgravity leads to reduced expression of the extracellular matrix protein, type I collagen (Col I). Conversely, modeled microgravity consistently increases Col I-specific alpha2 and beta1 integrin protein expression. Despite this increase in integrin sub-unit expression, autophosphorylation of adhesion-dependent kinases, focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2), is significantly reduced. Activation of Akt is unaffected by the reduction in FAK activation. However, reduced downstream signaling via the Ras-MAPK pathway is evidenced by a reduction in Ras and ERK activation. Taken together, our findings indicate that modeled microgravity decreases integrin/MAPK signaling, which likely contributes to the observed reduction in osteoblastogenesis.

Meyers, Valerie E.↗
NASA NTRS2007

Research Infrastructure Challenges for Graduate Programs in STEM Disciplines at Minority Institutions

It is much more challenging to perform experimental research functions at many minority institutions, because of lack of adequate research infrastructure. This is especially true if one wishes to initiate and implement masters and doctoral degree program in physics. In the present paper, an attempt is made to discuss the various hurdles encountered by the authors in the establishment of Master's and Doctoral degree programs in physics at one of the HBCUs (Historically Black Colleges and Universities). The department got no special or necessary treatment and faculty members are asked to teach as much course work as any other undergraduate department on the campus. It was very hard to convince university administration that giving less teaching load to research producing department faculty, shall culminate in abundant funding for the future years. This scenario created an extra heavy pressure on the faculty to continue the program. Some of the challenges included the resistance of some faculty and administrators to change, lack of sufficient release time for research producing faculty, and potential variation in funding or support with changes in the state education budget proration or members of the administration. In spite of the indirect cost recovery, very little infrastructure facilities was provided and the federal funding agencies did not want to interfere in the administration of the university. Various issues of recruiting and mentoring minority students, retention in the STEM disciplines as well as research infrastructure challenges at an HBCU university are presented.

Aggarwal, M. D.↗
NASA NTRS2012

NASA GSFC Opportunities for STEM Professionals Using the Vantage Point of Space

NASA has a variety of learning opportunities for STEM professionals. Three opportunities at GSFC are examined in this chapter: 1) standard summer research and development internship for undergraduates, 2) senior internship for undergraduate and graduate students and 3) a workshop series for informal learning center professionals. We describe these programs, examine their evolution with respect to most effective education practices and their assessment and evaluation, and identify the similarities and differences between them. The internship programs highlight authentic project-based research and development experiences with the senior internship providing a richer, deeper, and more demanding experience that has greater professional value. The workshops for informal learning center professionals on-the-other hand, focus on building knowledge of GSFC s science and engineering strengths among these professionals, and on building enduring partnerships between individuals (participants and GSFC scientist, engineers and educators) and between organizations (GSFC and the informal learning center). Finally, we examine the characteristics of these programs from a design and management perspective. Through this examination we identify a general structure that provides insight into more effective design and management of similar education programs.

Meeson, Blanche W.↗