Light-based control of metabolic flux through assembly of synthetic organelles
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We have systematically investigated the position, orientation, and polarization angle dependence of scattered light from well-characterized, indium tin oxide nanorods (ITO NRs) upon illumination with monochromatic light. Scattering signals from individual ITO NRs of horizontal and vertical configurations are probed quantitatively by examining signal response with respect to the analyzer angle and position along the length of the NR. Our efforts can be highly beneficial in providing fundamental understanding for the light interaction behavior of ITO NRs. Our results can provide valuable bases for comprehending optical emission from individual NRs, with their ever-growing applications in optoelectronics, photonics, and biosensing.
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The objective of this monitored field study was to identify near-term innovative façade technologies for solar control and daylighting with a goal of 20-40% energy use savings below Title 24 2013 in the 30-ft deep perimeter zone near vertical windows within cost and comfort constraints. The targeted market was new or existing commercial office buildings or buildings with similar patterns of use.
This paper describes experimental and analytical studies of the interior noise of twin-engine, propeller-driven, light aircraft. Experimental results indicate that interior noise levels due to propeller noise can be reduced by reduction of engine rpm at constant airspeed (about 3 dB), by synchronization of the twin engines/propellers (up to 12 dB), and by increasing the distances from propeller tip to fuselage. The analytical model described uses modal methods and incorporates the flat-sided geometrical and skin-stringer structural features of light aircraft. Initial results show good agreement with measured noise transmitted into a rectangular box through a flat panel.
Experimental and theoretical studies of the transmission loss (TL) of 1.15 x 1.46 m flat stiffened-skin aluminum panels are described. Panel configurations included panels with no treatment and eight combinations of treatments consisting of a fiberglass-septum layer, a foam-foil damping material, and a plywood double wall trim panel. Measured TL of the untreated panel and the panel with a damping layer showed characteristics of the double mass law: at low frequencies the TL followed a mass law trend associated with the average mass of both skin and stiffness, while at higher frequencies the TL followed a mass law trend associated with skin mass only. A comparison of TL for panels treated with a damping layer, a fiberglass layer, or a trim panel showed that the effects of damping are frequency dependent with some performing better below 500 Hz and others performing better above 500 Hz. Treatment combinations showed that two treatments with the same mass could have TL values different by about 10 dB, while other combinations having weights different by a factor of two could have TL values within a few dB over most of the frequency range. The highest TL values (50 dB at 1000 Hz) were obtained with a treatment consisting of a fiberglass layer, a trim panel, and damping layers on both the stiffened panel and the trim panel.
Previously cited in issue 24, p. 4127, Accession no. A81-48618
Constant output maintained, though luminance varies by 5 million to 1. Three means of normalizing video output utilized in video-level controller: iris adjustment, tube voltage adjustment, and automatic gain control. With aid of automatic light control and gain control, television camera accommodates maximum light level 5 million times greater than lowest light level, while outputting constant 3-V peak signal to processing circuitry.
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PoE technologies are currently ubiquitous in offices in the form of phones, access points, and IP security cameras and managed by on-site IT staff. As low voltage DC-power capabilities have increased with network switches, PoE devices are now being integrated across additional building systems, including lighting, computers, and HVAC controls. Since PoE devices are connected to network switches, energy monitoring and management can be performed through the IT network management software. Data and results from technology validations are still very limited for PoE equipment. This lack of information hinders higher adoption as its benefits and challenges have been insufficiently quantified in real-world scenarios. The use of IT network switches to power and control lighting, plug loads, and HVAC were demonstrated at a variety of sites including offices, schools, and a hotel. This paper presents the findings of a three-year DOE-/State of Minnesota-funded project that demonstrated and assessed the energy and non-energy benefits of Power over Ethernet (PoE) technologies at four commercial/institutional sites. The goals of the demonstrations were to: (1) assess the receptiveness of commercial and institutional markets to these new technologies, (2) demonstrate the feasibility of PoE technologies within standard design/construction practices and commercial codes, (3) assess the energy and cost savings opportunities of these technologies, (4) provide energy management opportunities where they typically are not available, and (5) formulate approaches to spur adoption of these technologies in the marketplace.
PoE technologies are currently ubiquitous in offices in the form of phones, access points, and IP security cameras and managed by on-site IT staff. As low voltage DC-power capabilities have increased with network switches, PoE devices are now being integrated across additional building systems, including lighting, computers, and HVAC controls. Since PoE devices are connected to network switches, energy monitoring and management can be performed through the IT network management software. Data and results from technology validations are still very limited for PoE equipment. This lack of information hinders higher adoption as its benefits and challenges have been insufficiently quantified in real-world scenarios. The use of IT network switches to power and control lighting, plug loads, and HVAC were demonstrated at a variety of sites including offices, schools, and a hotel. This paper presents the findings of a three-year DOE-/State of Minnesota-funded project that demonstrated and assessed the energy and non-energy benefits of Power over Ethernet (PoE) technologies at four commercial/institutional sites. The goals of the demonstrations were to: (1) assess the receptiveness of commercial and institutional markets to these new technologies, (2) demonstrate the feasibility of PoE technologies within standard design/construction practices and commercial codes, (3) assess the energy and cost savings opportunities of these technologies, (4) provide energy management opportunities where they typically are not available, and (5) formulate approaches to spur adoption of these technologies in the marketplace.
Case study describes how the Byron G. Rogers Federal Building replaced existing T12 and T8 luminaires with LED dedicated troffers with advanced controls. Together these measures cut lighting energy use by nearly 60% in the 18-story, 791,000-square-foot facility. The new lighting control system provides automated dimming of lights down to 0% output when daylight provides adequate light levels. The project earned GSA an award for exemplary performance from the Interior Lighting Campaign in 2016.
Electronic controller switches lights on or off in response to amount of sunlight available. Is application in offices and industrial installations where electrical energy is wasted by using artificial light in sunlit areas. Device utilizes electronic monitor that varies artificial lighting according to amount of sunlight in given area.
In this project we combined synthetic biology, systems biology, protein engineering and metabolic engineering to study, control, and improve the production of branched chain alcohols (BCAs), a class of advanced biofuels preferred by the DOE, in the yeast Saccharomyces cerevisiae. This involved the development and application of optogenetic systems as a new modality of dynamic control of native and engineered metabolic pathways, using light as inducible or repressible agent. The optogenetic systems include gene circuits for light control of gene expression, as well as light-assembled synthetic organelles and photo-switchable protein binders to control metabolic and protein function with light at the protein level. In addition, we developed the first genetically encoded biosensor for BCA production in yeast, which we used to design high throughput assays to identify highly productive strains, pathways, and enzymes. We also showed that this biosensor can be functionally co-expressed with optogenetic circuits in the same strain, raising the possibility of establishing, for the first time, computer-interfaced closed-loop controls of engineered metabolic pathways. The yeast gene deletion library was also utilized to conduct the first systems-level study on BCA toxicity in yeast, which uncovered key fundamental principles of yeast sensitivity and tolerance to these alcohols, allowing us to design highly tolerant strains with increased BCA production. This project, thus comprises the development of several new technologies, which we integrated to make new discoveries on the dynamics of BCA production and their mechanisms of cellular toxicity and tolerance, as well as to establish new paradigms to engineer and control metabolic pathways and microbial fermentations with light, for the production of BCAs and other products of interest to the DOE.
Sleep inertia is the brief period of performance impairment and reduced alertness experienced after waking, especially from slow-wave sleep. We assessed the efficacy of polychromatic short-wavelength-enriched light to improve vigilant attention, alertness and mood immediately after waking from slow-wave sleep at night. Twelve participants (six female, 23.3 ± 4.2 years) maintained an actigraphy-confirmed sleep schedule of 8.5 hr for 5 nights, and 5 hr for 1 night prior to an overnight laboratory visit. In the laboratory, participants were awakened from slow-wave sleep, and immediately exposed to either dim, red ambient light (control) or polychromatic short-wavelength-enriched light (light) for 1 hr in a randomized crossover design. They completed a 5-min Psychomotor Vigilance Task, the Karolinska Sleepiness Scale, and Visual Analogue Scales of mood at 2, 17, 32 and 47 min after waking. Following this testing period, lights were turned off and participants returned to sleep. They were awakened from their subsequent slow-wave sleep period and received the opposite condition. Compared with the control condition, participants exposed to light had fewer Psychomotor Vigilance Task lapses (χ 2 [1] = 5.285, p = 0.022), reported feeling more alert (Karolinska Sleepiness Scale: F 1,77 = 4.955, p = 0.029; Visual Analogue Scale alert : F 1,77 = 8.226, p = 0.005), and reported improved mood (Visual Analogue Scale cheerful : F 1,77 = 8.615, p = 0.004). There was no significant difference in sleep-onset latency between conditions following the testing period (t 10 = 1.024, p = 0.330). Our results suggest that exposure to polychromatic short-wavelength-enriched light immediately after waking from slow-wave sleep at night may help improve vigilant attention, subjective alertness, and mood. Future studies should explore the potential mechanisms of this countermeasure and its efficacy in real-world environments.