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At least 163 records · Page 9

Large fluxes of fatty acids from membranes to triacylglycerol and back during N-deprivation and recovery in Chlamydomonas

Abstract Microalgae accumulate triacylglycerol (TAG) during nutrient deprivation and break it down after nutrient resupply, and these processes involve dramatic shifts in cellular carbon allocation. Due to the importance of algae in the global carbon cycle, and the potential of algal lipids as feedstock for chemical and fuel production, these processes are of both ecophysiological and biotechnological importance. However, the metabolism of TAG is not well understood, particularly the contributions of fatty acids (FAs) from different membrane lipids to TAG accumulation and the fate of TAG FAs during degradation. Here, we used isotopic labeling time course experiments on Chlamydomonas reinhardtii to track FA synthesis and transfer between lipid pools during nitrogen (N)-deprivation and resupply. When cells were labeled before N-deprivation, total levels of label in cellular FAs were unchanged during subsequent N-deprivation and later resupply, despite large fluxes into and out of TAG and membrane lipid pools. Detailed analyses of FA levels and labeling revealed that about one-third of acyl chains accumulating in TAG during N-deprivation derive from preexisting membrane lipids, and in total, at least 45% of TAG FAs passed through membrane lipids at one point. Notably, most acyl chains in membrane lipids during recovery after N-resupply come from TAG. Fluxes of polyunsaturated FAs from plastidic membranes into TAG during N-deprivation were particularly noteworthy. These findings demonstrate a high degree of integration of TAG and membrane lipid metabolism and highlight a role for TAG in storage and supply of membrane lipid components.

Plant Sciences↗

Data for "Quantifying the Propagation of Parametric Uncertainty on Flux Balance Analysis"

In the repository are example scripts that perform uncertainty injection and propagation to flux balance analysis with outputs for a small sample size (for demonstration purpose only). For proper analysis, user should download the scripts and run for a large sample size (e.g., 10,000 samples). If you use the scripts, please cite the following Metabolic Engineering article: “Quantifying the propagation of parametric uncertainty on flux balance analysis” (https://doi.org/10.1016/j.ymben.2021.10.012) There are two subdirectories: /uncFBA/uncBiom: injection of normally distributed noise to biomass precursor coeffcients and ATP maintenance (growth-associated ATP maintenance (GAM) and non-growth associated ATP maintenance (NGAM)) /uncFBA/uncRHS: departure from steady-state by adding noise drawn from normal distribution to the RHS terms of mass balance constraints

Metabolomics↗

Critical Heat Flux Phenomena at High Pressure & Low Mass Fluxes: NEUP Final Report Part I: Experiments

This report is a preliminary document presenting an overview of the Critical Heat Flux (CHF) phenomenon, the High Pressure Critical Heat Flux facility (HPCHF), preliminary CHF data acquired, and the future direction of the research. The HPCHF facility has been designed and built to study CHF at high pressure and low mass flux ranges in a rod bundle prototypical of conceptual Small Modular Reactor (SMR) designs. The rod bundle is comprised of four electrically heated rods in a 2x2 square rod bundle with a prototypic chopped-cosine axial power profile and equipped with thermocouples at various axial and circumferential positions embedded in each rod for CHF detection. Experimental test parameters for CHF detection range from pressures of ~80 – 160 bar, mass fluxes of ~400 – 1500 kg/m 2 s, and inlet water subcooling from ~30 – 70°C. The preliminary data base established will be further extended in the future along with comparisons to existing CHF correlations, models, etc. whose application ranges may be applicable to the conditions of SMRs.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Effects of dynamic heat fluxes on model climate sensitivity Meridional sensible and latent heat fluxes

The high- and low-latitude radiative-dynamic (HLRD) climatic model of Wang et al. (1984) was used to study the effect of meridional heat (MH) fluxes on climate changes caused by increases of CO2 abundance and solar constant variations. However, the empirical MH parameterization of the HLRD model was replaced by physically based parameterization, which gives separate meridional sensible and latent heat fluxes and provides a complete representation of the dependence of the flux on the mean temperature field. Both parameterization methods yielded about the same changes in global mean surface temperature and ice line, and both produced only small changes in meridional temperature gradient, although the latter were even smaller with the physically based parameterizations. At any latitude, the hemispheric mean surface temperature, rather than MH fluxes, dominates the surface temperature changes.

Gutowski, W. J., Jr.↗

Comparison of flux-vector and flux-difference splitting techniques for hypersonic flow

Some numerical aspects of flux-vector splitting (FVS) and flux-difference splitting (FDS) schemes are investigated to determine the accuracy for the shock and expansion waves in hypersonic blunt body flow fields. The analysis includes the implementation of Steger-Warming's three-component FVS (Reklis and Thomas, 1981), van Leer's (1986) FVS, and Yang's (1985) FDS to the right-hand side of a time marching scheme where the left-hand side is a variant of the incremental line Gauss-Seidel scheme. Numerous computations are performed by blending two simple flux limiters to test the various schemes on a generic aerobrake at M(infinity) = 10 and zero angle of attack. It is found that the flux limiter plays a very important role in the accuracy of shock capturing. The steady state results are compared to those of a shock-fitting scheme and advantages and disadvantages of each scheme are discussed briefly.

Wey, T. C.↗

Permanent magnet flux-biased magnetic actuator with flux feedback

The invention is a permanent magnet flux-biased magnetic actuator with flux feedback for adjustably suspending an element on a single axis. The magnetic actuator includes a pair of opposing electromagnets and provides bi-directional forces along the single axis to the suspended element. Permanent magnets in flux feedback loops from the opposing electromagnets establish a reference permanent magnet flux-bias to linearize the force characteristics of the electromagnets to extend the linear range of the actuator without the need for continuous bias currents in the electromagnets.

Groom, Nelson J.↗

Accuracy of quantum sensors measuring yield photon flux and photosynthetic photon flux

Photosynthesis is fundamentally driven by photon flux rather than energy flux, but not all absorbed photons yield equal amounts of photosynthesis. Thus, two measures of photosynthetically active radiation have emerged: photosynthetic photon flux (PPF), which values all photons from 400 to 700 nm equally, and yield photon flux (YPF), which weights photons in the range from 360 to 760 nm according to plant photosynthetic response. We selected seven common radiation sources and measured YPF and PPF from each source with a spectroradiometer. We then compared these measurements with measurements from three quantum sensors designed to measure YPF, and from six quantum sensors designed to measure PPF. There were few differences among sensors within a group (usually <5%), but YPF values from sensors were consistently lower (3% to 20%) than YPF values calculated from spectroradiometric measurements. Quantum sensor measurements of PPF also were consistently lower than PPF values calculated from spectroradiometric measurements, but the differences were <7% for all sources, except red-light-emitting diodes. The sensors were most accurate for broad-band sources and least accurate for narrow-band sources. According to spectroradiometric measurements, YPF sensors were significantly less accurate (>9% difference) than PPF sensors under metal halide, high-pressure sodium, and low-pressure sodium lamps. Both sensor types were inaccurate (>18% error) under red-light-emitting diodes. Because both YPF and PPF sensors are imperfect integrators, and because spectroradiometers can measure photosynthetically active radiation much more accurately, researchers should consider developing calibration factors from spectroradiometric data for some specific radiation sources to improve the accuracy of integrating sensors.

NASA Center KSC↗

Chloride Flux Growth of Idiomorphic AWO 4 (A = Sr, Ba) Single Microcrystals

Scheelite-type divalent metal tungstate materials (AWO 4 ) have been studied for various applications due to their attractive mechanical and chemical properties. Preparation of the shape-controlled AWO 4 crystals with high crystallinity is one of the most effective approaches for further exploring and improving their properties. In this study, highly crystalline SrWO 4 and BaWO 4 microcrystals with different morphologies were grown by using a chloride flux growth technique. To investigate the effect of growth conditions on SrWO 4 and BaWO 4 crystals, NaCl and KCl were used as a flux, and the solute concentration was adjusted in the range of 5-50 mol %. The difference in the flux cation species (Na + and K + ) mainly affected the crystal size. In accordance with increasing the solute concentration, the dominant crystal shape of SrWO 4 and BaWO 4 varied as follows: whisker (a rod- or wire-like morphology with a large aspect ratio) → platelet → well/less-faceted polyhedron. Additionally, according to scanning electron microscopy and transmission electron microscopy results, a dendritic morphological transformation from AWO4 whisker to platelet during crystal growth has been proposed.

36 MATERIALS SCIENCE↗

Ion fluxes and memory effects in an Ar–O 2 modulated radiofrequency-driven atmospheric pressure plasma jet

In this work we used molecular beam mass spectrometry to characterize ion fluxes produced by a modulated radiofrequency (RF)-driven atmospheric pressure plasma jet operating in a homogeneous gas environment (Ar + 1% O 2 ). The influence of the RF modulation frequency (100 Hz to 20 kHz) on the ion fluxes was investigated by time-resolved measurements, and the lifetimes of the dominant ions, O 2 + , NO + , O − , O 2 − and O 3 − , were found to be 28 ± 2, 117 ± 8, 7.3 ± 0.4, 17 ± 1 and 23 ± 2 μs, respectively. The absolute ion densities in the near afterglow region were found to be of the order of 1011 cm−3. Significant differences in the dynamics of the positive and negative ions were found; these are explained by the presence of electrons at similar densities in the afterglow produced by electron detachment reactions from negative ions due to the large concentrations of atomic oxygen and singlet delta oxygen. Transitions in ion flux dynamics for different modulation frequencies and at the startup of the plasma were analyzed together with intensified charge coupled device images recording the plasma propagation in order to assess the dynamics of plasma plume propagation and how it is impacted by ‘memory effects’. Quantitative measurements of the ion densities enabling these memory effects are reported. The results in this paper highlight the tremendous impact of memory effects on plasma propagation and the corresponding pre-ionization.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

As-Built Simulation of the High Flux Isotope Reactor

The Oak Ridge National Laboratory High Flux Isotope Reactor (HFIR) is an 85 MWt flux trap-type research reactor that supports key research missions, including isotope production, materials irradiation, and neutron scattering. The core consists of an inner and an outer fuel element containing 171 and 369 involute-shaped plates, respectively. The thin fuel plates consist of a U 3 O 8 -Al dispersion fuel (highly enriched), an aluminum-based filler, and aluminum cladding. The fuel meat thickness is varied across the width of the involute plate to reduce thermal flux peaks at the radial edges of the fuel elements. Some deviation from the designed fuel meat shaping is allowed during manufacturing. A homogeneity scan of each fuel plate checks for potential anomalies in the fuel distribution by scanning the surface of the plate and comparing the attenuation of the beam to calibration standards. While typical HFIR simulations use homogenized fuel regions, explicit models of the plates were developed under the Low-Enriched Uranium Conversion Program. These explicit models typically include one inner and one outer fuel plate with nominal fuel distributions, and then the plates are duplicated to fill the space of the corresponding fuel element. Therefore, data extracted from these simulations are limited to azimuthally averaged quantities. To determine the reactivity and physics impacts of an as-built outer fuel element and generate azimuthally dependent data in the element, 369 unique fuel plate models were generated and positioned. This model generates the three-dimensional (i.e., radial–axial–azimuthal) plate power profile, where the azimuthal profile is impacted by features within the adjacent control element region and beryllium reflector. For an as-built model of the outer fuel element, plate-specific homogeneity data, 235 U loading, enrichment, and channel thickness measurements were translated into the model, yielding a much more varied azimuthal power profile encompassed by uncertainty factors in analyses. These models were run with the ORNL-TN and Shift Monte Carlo tools, and they contained upwards of 500,000 cells and 100,000 unique tallies.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Survival of Flux Transfer Event (FTE) Flux Ropes Far Along the Tail Magnetopause

During intervals of southward IMF, magnetic reconnection can result in the formation of flux transfer events (FTEs) on the dayside magnetopause which travel along the magnetopause in the anti-sunward direction. Of particular interest is their fate and the role they play transporting solar wind plasma into the magnetosphere. We present the discovery of FTEs far along the distant tail magnetopause (x = 67 Earth radii) using data from ARTEMIS on the dusk flank magnetopause under southward/duskward IMF conditions. The identification of several events is further supported by excellent fits to a force-free flux rope model. The axis of each structure is principally north-south, i.e., perpendicular to the Sun-Earth line. Simultaneous observations by THEMIS on the dayside magnetopause indicate that FTEs are being produced there, although perhaps 2-4 times smaller in size. The convection time from the dayside magnetopause to ARTEMIS is 30 min, and the FTEs have a flux content comparable to those typically observed on the dayside magnetopause, indicating that these features are in quasi-equilibrium as they are convected downtail. By considering the relative orientations of the FTEs observed by THEMIS and ARTEMIS, the magnetic field geometry is consistent with the FTEs being produced on the dayside magnetopause along an extended X-line in the presence of IMF By and bending as they are convected to the flanks.

ARTEMIS↗

Intrinsic Constraints on Asymmetric Turbulent Transport of Scalars Within the Constant Flux Layer of the Lower Atmosphere: CONSTANT FLUX LAYER

A widely used assumption in boundary layer meteorology is the z independence of turbulent scalar fluxes F s throughout the atmospheric surface layer, where z is the distance from the boundary. This assumption is necessary for the usage of Monin-Obukhov Similarity Theory and for the interpretation of eddy covariance measurements of F s when using them to represent emissions or uptake from the surface. It is demonstrated here that the constant flux assumption offers intrinsic constraints on the third-order turbulent transport of F s in the unstable atmospheric surface layer. When enforcing z independence of F s on multilevel F s measurements collected above different surface cover types, it is shown that increasing instability leads to a novel and universal description of (i) the imbalance between ejecting and sweeping eddy contributions to F s and (ii) the ratio formed by a dimensionless turbulent transport of Fs and a dimensionless turbulent transport of scalar variance. When combined with structural models for the turbulent transport of F s , these two findings offer a new perspective on “closing” triple moments beyond conventional gradient diffusion schemes. A practical outcome is a diagnostic of the constant flux assumption from single-level F s measurements.

54 ENVIRONMENTAL SCIENCES↗

A mechanism for the dynamo terms to sustain closed-flux current, including helicity balance, by driving current which crosses the magnetic field

An analysis of imposed dynamo current drive (IDCD) [T.R. Jarboe et al., Nucl. Fusion 52 083017 (2012)] reveals: (a) current drive on closed flux surfaces seems possible without relaxation, reconnection, or other flux-surface-breaking large events; (b) the scale size of the key physics may be smaller than is often computationally resolved; (c) helicity can be sustained across closed flux; and (d) IDCD current drive is parallel to the current which crosses the magnetic field to produce the current driving force. In addition to agreeing with spheromak data, IDCD agrees with selected tokamak data.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Error field impact on mode locking and divertor heat flux in NSTX-U

During the 2016 NSTX-U experimental campaign, locked modes in the plasma edge presented clear evidence of the presence of error fields. Extensive metrology and plasma response modeling with IPEC and M3D-C1 have been conducted to understand the various sources of error fields in NSTX-U as built in 2016, and to determine which of these sources have the greatest effect on the plasma. In particular, modeling finds that the error field from misalignment of the toroidal field (TF) coils may have a significant effect on the plasma. The response to the TF error field is shown to depend on the presence of a q = 1 surface, in qualitative agreement with experimental observations. It is found that certain characteristics of the TF error field present new challenges for error field correction. Specifically, the error field spectrum differs significantly from that of coils on the low-field side (such as the NSTX-U error field correction coils), and does not resonate strongly with the dominant kink mode, thus potentially requiring a multi-mode correction. Moreover, to mitigate heat fluxes using poloidal flux expansion, the pitch angle at the divertor plates must be small (~1°). It is shown that uncorrected error fields may result in potentially significant local perturbation to the pitch angle. Estimates for coil alignment tolerances in NSTX-U are derived based on consideration of both heat flux and core resonant fields independently.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Hacking the thylakoid proton motive force for improved photosynthesis: modulating ion flux rates that control proton motive force partitioning into Δ ψ and ΔpH

There is considerable interest in improving plant productivity by altering the dynamic responses of photosynthesis in tune with natural conditions. This is exemplified by the 'energy-dependent' form of non-photochemical quenching (q E ), the formation and decay of which can be considerably slower than natural light fluctuations, limiting photochemical yield. In addition, we recently reported that rapidly fluctuating light can produce field recombination-induced photodamage (FRIP), where large spikes in electric field across the thylakoid membrane (Δψ) induce photosystem II recombination reactions that produce damaging singlet oxygen ( 1 O 2 ). Both q E and FRIP are directly linked to the thylakoid proton motive force (pmf), and in particular, the slow kinetics of partitioning pmf into its ΔpH and Δψ components. In this work, using a series of computational simulations, we explored the possibility of 'hacking' pmf partitioning as a target for improving photosynthesis. Under a range of illumination conditions, increasing the rate of counter-ion fluxes across the thylakoid membrane should lead to more rapid dissipation of Δψ and formation of ΔpH. This would result in increased rates for the formation and decay of qE while resulting in a more rapid decline in the amplitudes of Δψ-spikes and decreasing 1 O 2 production. These results suggest that ion fluxes may be a viable target for plant breeding or engineering. However, these changes also induce transient, but substantial mismatches in the ATP : NADPH output ratio as well as in the osmotic balance between the lumen and stroma, either of which may explain why evolution has not already accelerated thylakoid ion fluxes. Overall, though the model is simplified, it recapitulates many of the responses seen in vivo, while spotlighting critical aspects of the complex interactions between pmf components and photosynthetic processes. By making the programme available, we hope to enable the community of photosynthesis researchers to further explore and test specific hypotheses. This article is part of the themed issue 'Enhancing photosynthesis in crop plants: targets for improvement'.

59 BASIC BIOLOGICAL SCIENCES↗

Radiative Flux Analysis (RADFLUXANAL) Value-Added Product: Retrieval of Clear-Sky Broadband Radiative Fluxes and Other Derived Values

This technical report describes the algorithms used in the Radiative Flux Analysis (RADFLUXANAL) Value-Added Product (VAP). This VAP estimates clear sky broadband irradiance values and derives several additional cloud properties from broadband measurements including fractional sky cover and cloud optical depth. The VAP is based on a collection of algorithms and codes developed by Charles N. Long. It is an updated version of the Shortwave Flux Analysis VAP that has been used in ARM for a number of years. The RADFLUXANAL VAP includes two major updates over the previous Shortwave Flux Analysis VAP: 1) longwave (LW) clear sky irradiance estimates and derived parameters, and 2) takes in better quality-controlled input data from the QCRAD VAP.

54 ENVIRONMENTAL SCIENCES↗