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At least 163 records · Page 9

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NASA NTRS2012

Low Biotoxicity of Mars Analog Soils Suggests that the Surface of Mars May be Habitable for Terrestrial Microorganisms

Recent studies on the interactive effects of hypobaria, low temperatures, and CO2-enriched anoxic atmospheres on the growth of 37 species of mesophilic bacteria identified 14 potential biocidal agents that might affect microbial survival and growth on the martian surface. Biocidal or inhibitory factors include (not in priority): (1) solar UV irradiation, (2) low pressure, (3) extreme desiccating conditions, (4) extreme diurnal temperature fluctuations, (5) solar particle events, (6) galactic cosmic rays, (7) UV-glow discharge from blowing dust, (8) solar UV-induced volatile oxidants [e.g., O2(-), O(-), H2O2, O3], (9) globally distributed oxidizing soils, (10) extremely high salts levels [e.g., MgCl2, NaCl, FeSO4, and MgSO4] in surficial soils at some sites on Mars, (11) high concentrations of heavy metals in martian soils, (12) likely acidic conditions in martian fines, (13) high CO2 concentrations in the global atmosphere, and (14) perchlorate-rich soils. Despite these extreme conditions several studies have demonstrated that dormant spores or vegetative cells of terrestrial microorganisms can survive simulated martian conditions as long as they are protected from UV irradiation. What has not been explored in depth are the effects of potential biotoxic geochemical components of the martian regolith on the survival and growth of microorganisms. The primary objectives of the research included: (1) prepare and characterize Mars analog soils amended with potential biotoxic levels of sulfates, salts, acidifying minerals, etc.; and (2) use the simulants to conduct biotoxicity assays to determine if terrestrial microorganisms from spacecraft can survive direct exposure to the analog soils.

Schuerger, A. C.↗

NASA NTRS2013

Overview of International Space Station Carbon Dioxide Removal Assembly On-Orbit Operations and Performance

Controlling Carbon Dioxide (CO2) partial pressure in the habitable vehicle environment is a critical part of operations on the International Space Station (ISS). On the United States segment of ISS, CO2 levels are primarily controlled by the Carbon Dioxide Removal Assembly (CDRA). There are two CDRAs on ISS; one in the United States Laboratory module, and one in the Node3 module. CDRA has been through several significant operational issues, performance issues and subsequent re-design of various components, primarily involving the Desiccant Adsorbent Bed (DAB) assembly and Air Selector Valves (ASV). This paper will focus on significant operational and performance issues experienced by the CDRA team from 2008-2012.

Matty, Christopher M.↗

NASA NTRS2012

International Space Station Environmental Control and Life Support System Status: 2012-2013

Williams, David E.↗

NASA NTRS2011

Assessment of Resistance of Bacillus Horneckiae Endospores to UV Radiation and Function of Their Extraneous Layer in Resistance

Spore-forming microbes are highly resistant to various physical and chemical conditions, which include ionizing and UV radiation, desiccation and oxidative stress, and the harsh environment of outer space or planetary surfaces. The spore's resistance might be due to their metabolically dormant state, and/or by the presence of a series of protective structures that encase the interior-most compartment, the core, which houses the spore chromosome. These spores have multiple layers surrounding the cell that are not found in vegetative cells, and some species have an outer layer of proteins and glycoproteins termed the "exosporium" or a fibrous "extraneous layer" (EL). Bacillus horneckiae is an EL-producing novel sporeformer isolated from a Phoenix spacecraft assembly clean room, and it has previously demonstrated resistance to UV radiation up to 1000 J/m(sup 2). The EL appears to bind B. horneckiae spores into large aggregations, or biofilms, and may confer some UV resistance to the spores. Multiple culturing and purification schemes were tried to achieve high purity spores because vegetative cells would skew UV resistance results. An ethanol-based purification scheme produced high purity spores. Selective removal of the EL from spores was attempted with two schemes: a chemical extraction method and physical extraction (sonication). Results from survival rates in the presence and absence of the external layer will provide a new understanding of the role of biofilms and passive resistance that may favor survival of biological systems in aggressive extra-terrestrial environments. The chemical extraction method decreased viable counts of spores and lead to an inconclusive change UV resistance relative to non-extracted spores. The physical extraction method lead to non-aggregated spores and did not alter viability; however, it produced UV resistance profiles similar to non-extracted spores. In addition to the EL-removal study, samples of B. horneckiae spores dried on aluminum coupons and exposed to increasing UV (200-400 nm range) levels (0 to 8.0 x 105 kJ/m(sup 2)) were tested for viability, which indicated that the maximum UV exposure level that still resulted in viable spores was 5.0 x 10? kJ/m(sup 2).

resistance↗

NASA NTRS2013

Preparation of a Frozen Regolith Simulant Bed for ISRU Component Testing in a Vacuum Chamber

In-Situ Resource Utilization (ISRU) systems and components have undergone extensive laboratory and field tests to expose hardware to relevant soil environments. The next step is to combine these soil environments with relevant pressure and temperature conditions. Previous testing has demonstrated how to incorporate large bins of unconsolidated lunar regolith into sufficiently sized vacuum chambers. In order to create appropriate depth dependent soil characteristics that are needed to test drilling operations for the lunar surface, the regolith simulant bed must by properly compacted and frozen. While small cryogenic simulant beds have been created for laboratory tests, this scale effort will allow testing of a full 1m drill which has been developed for a potential lunar prospector mission. Compacted bulk densities were measured at various moisture contents for GRC-3 and Chenobi regolith simulants. Vibrational compaction methods were compared with the previously used hammer compaction, or "Proctor", method. All testing was done per ASTM standard methods. A full 6.13 m3 simulant bed with 6 percent moisture by weight was prepared, compacted in layers, and frozen in a commercial freezer. Temperature and desiccation data was collected to determine logistics for preparation and transport of the simulant bed for thermal vacuum testing. Once in the vacuum facility, the simulant bed will be cryogenically frozen with liquid nitrogen. These cryogenic vacuum tests are underway, but results will not be included in this manuscript.

Klenhenz, Julie↗

NASA NTRS2014

Martian Oceans: Old Debate - New Insights

The possibility of an ancient ocean in the northern lowlands of Mars has been discussed for decades [1-14], but the subject remains controversial [15-20]. Among the many unique features of the northern lowlands is the extensive development of "giant polygons" - polygonal landforms that range from 1 to 20 km across. The kilometer-scale size of these features distinguishes them from a variety of smaller polygons (usually < 250 m) on Mars that have been compared to terrestrial analogs such as ice-wedge and desiccation features. However, until recently, geologists were aware of no examples of polygons on Earth comparable in scale to the giant polygons of Mars, so there were no good analogs from which to draw interpretations. That picture has changed with 3D seismic data acquired by the petroleum industry in exploration of offshore basins. The new data reveal kilometer-scale polygonal features in more than 50 offshore basins on Earth]. These features provide a credible analog for the giant polygons of Mars.

Oehler, Dorothy Z.↗

NASA NTRS2014

Lunar Polar Environmental Testing: Regolith Simulant Conditioning

As ISRU system development approaches flight fidelity, there is a need to test hardware in relevant environments. Extensive laboratory and field testing have involved relevant soil (lunar regolith simulants), but the current design iterations necessitate relevant pressure and temperature conditions. Including significant quantities of lunar regolith simulant in a thermal vacuum chamber poses unique challenges. These include facility operational challenges (dust tolerant hardware) and difficulty maintaining a pre-prepared soil state during pump down (consolidation state, moisture retention).For ISRU purposes, the regolith at the lunar poles will be of most interest due to the elevated water content. To test at polar conditions, the regolith simulant must be doped with water to an appropriate percentage and then chilled to cryogenic temperatures while exposed to vacuum conditions. A 1m tall, 28cm diameter bin of simulant was developed for testing these simulant preparation and drilling operations. The bin itself was wrapped with liquid nitrogen cooling loops (100K) so that the simulant bed reached an average temperature of 140K at vacuum. Post-test sampling was used to determine desiccation of the bed due to vacuum exposure. Depth dependent moisture data is presented from frozen and thawed soil samples.Following simulant only evacuation tests, drill hardware was incorporated into the vacuum chamber to test auguring techniques in the frozen soil at thermal vacuum conditions. The focus of this testing was to produce cuttings piles for a newly developed spectrometer to evaluate. This instrument, which is part of the RESOLVE program science hardware, detects water signatures from surface regolith. The drill performance, behavior of simulant during drilling, and characteristics of the cuttings piles will be offered.

lunar regolith↗

NASA NTRS2014

Salt Tolerance and Polyphyly in the Cyanobacterium Chroococcidiopsis (Pleurocapsales)1

Chroococcidiopsis Geitler (Geitler 1933) is a genus of cyanobacteria containing desiccation and radiation resistant species. Members of the genus live in habitats ranging from hot and cold deserts to fresh and saltwater environments. Morphology and cell division pattern have historically been used to define the genus. To better understand the genetic and phenotypic diversity of the genus, 15 species were selected that had been previously isolated from different locations, including salt and freshwater environments. Four markers were sequenced from these 15 species, the 16S rRNA, rbcL, desC1 and gltX genes. Phylogenetic trees were generated which identified two distinct clades, a salt-tolerant clade and a freshwater clade. This study demonstrates that the genus is polyphyletic based on saltwater and freshwater phenotypes. To understand the resistance to salt in more details, species were grown on a range of sea salt concentrations which demonstrated that the freshwater species were salt-intolerant whilst the saltwater species required salt for growth. This study shows an increased resolution of the phylogeny of Chroococcidiopsis and provides further evidence that the genus is polyphyletic and should be reclassified to improve clarity in the literature.

Genus↗

NASA NTRS2014

Lunar Polar Environmental Testing: Regolith Simulant Conditioning

in situ resource utilization↗

NASA NTRS2014

Microorganisms in the Stratosphere (MIST): In-flight Sterilization with UVC Leds

The stratosphere (10 km to 50 km above sea level) is a unique place on Earth for astrobiological studies of microbes in extreme environments due to the combination of harsh conditions (high ultraviolet radiation, low pressure, desiccation, and low temperatures). Microorganisms in the Stratosphere (MIST) will attempt to characterize the diversity of microbes at these altitudes using a balloon collection device on a meteorological weather balloon. A major challenge of such an aerobiology study is the potential for ground contamination that makes it difficult to distinguish between collected microbes and contaminants. One solution is to use germicidal ultraviolet light emitting diodes (UV LEDs) to sterilize the collection strip. To use this solution, an optimal spatial arrangement of the lights had to be determined to ensure the greatest chance of complete sterilization within the 30 to 60 minute time of balloon ascent. A novel, 3D-printed test stand was developed to experimentally determine viable Bacillus pumilus SAFR-032 spore reduction after exposure to ultraviolet radiation at various times, angles, and distances. Taken together, the experimental simulations suggested that the UV LEDs on the MIST flight hardware should be active for at least 15 minutes and mounted within 4 cm of the illuminated surface at any angle to achieve optimal sterilization. These findings will aid in the production of the balloon collection device to ensure pristine stratospheric microbial samples are collected. Flight hardware capable of in-flight self-sterilization will enable future life detection missions to minimize both forward contamination and false positives.

Exobiology↗

NASA NTRS2014

Modeling of Revitalization of Atmospheric Water

The Atmosphere Revitalization Recovery and Environmental Monitoring (ARREM) project was initiated in September of 2011 as part of the Advanced Exploration Systems (AES) program. Under the ARREM project, testing of sub-scale and full-scale systems has been combined with multiphysics computer simulations for evaluation and optimization of subsystem approaches. In particular, this paper describes the testing and modeling of the water desiccant subsystem of the carbon dioxide removal assembly (CDRA). The goal is a full system predictive model of CDRA to guide system optimization and development.

Coker, Robert↗

NASA NTRS2014

Biosentinel: Developing a Space Radiation Biosensor

Ionizing radiation presents a major challenge to human exploration and long-term residence in space. The deep-space radiation spectrum includes highly energetic particles that generate double strand breaks (DSBs), deleterious DNA lesions that are usually repaired without errors via homologous recombination (HR), a conserved pathway in all eukaryotes. While progress identifying and characterizing biological radiation effects using Earth-based facilities has been significant, no terrestrial source duplicates the unique space radiation environment.We are developing a biosensor-based nanosatellite to fly aboard NASAs Space Launch System Exploration Mission 1, expected to launch in 2017 and reach a 1AU (astronomic unit) heliocentric orbit. Our biosensor (called BioSentinel) uses the yeast S. cerevisiae to measure DSBs in response to ambient space radiation. The BioSentinel strain contains engineered genetic defects that prevent growth until and unless a radiation-induced DSB near a reporter gene activates the yeasts HR repair mechanisms. Thus, culture growth and metabolic activity directly indicate a successful DSB-and-repair event. In parallel, HR-defective and wild type strains will provide survival data. Desiccated cells will be carried within independent culture microwells, built into 96-well microfluidic cards. Each microwell set will be activated by media addition at different time points over 18 months, and cell growth will be tracked continuously via optical density. One reserve set will be activated only in the occurrence of a solar particle event. Biological measurements will be compared to data provided by onboard physical dosimeters and to Earth-based experiments.BioSentinel will conduct the first study of biological response to space radiation outside Low Earth Orbit in over 40 years. BioSentinel will thus address strategic knowledge gaps related to the biological effects of space radiation and will provide an adaptable platform to perform human-relevant measurements in multiple space environments. We hope that it can therefore be used on the ISS, on and around other planetary bodies as well as other exploration platforms as a self-contained system that will allow us to compare and calibrate different radiation environments.BioSentinels results will be critical for improving interpretation of the effects of space radiation exposure, and for reducing the risk associated with long-term human exploration.

space radiation↗

NASA NTRS2014

Ground Testing of the EMCS Seed Cassette for Biocompatibility with the Cellular Slime Mold, Dictyostelium Discoideum

The European Modular Cultivation System, EMCS, was developed by ESA for plant experiments. To expand the use of flight verified hardware for various model organisms, we performed ground experiments to determine whether ARC EMCS Seed Cassettes could be adapted for use with cellular slime mold for future space flight experiments. Dictyostelium is a cellular slime mold that can exist both as a single-celled independent organism and as a part of a multicellular colony which functions as a unit (pseudoplasmodium). Under certain stress conditions, individual amoebae will aggregate to form multicellular structures. Developmental pathways are very similar to those found in Eukaryotic organisms, making this a uniquely interesting organism for use in genetic studies. Dictyostelium has been used as a genetic model organism for prior space flight experiments. Due to the formation of spores that are resistant to unfavorable conditions such as desiccation, Dictyostelium is also a good candidate for use in the EMCS Seed Cassettes. The growth substratum in the cassettes is a gridded polyether sulfone (PES) membrane. A blotter beneath the PES membranes contains dried growth medium. The goals of this study were to (1) verify that Dictyostelium are capable of normal growth and development on PES membranes, (2) develop a method for dehydration of Dictyostelium spores with successful recovery and development after rehydration, and (3) successful mock rehydration experiments in cassettes. Our results show normal developmental progression in two strains of Dictyostelium discoideum on PES membranes with a bacterial food source. We have successfully performed a mock rehydration of spores with developmental progression from aggregation to slug formation, and production of morphologically normal spores within 9 days of rehydration. Our results indicate that experiments on the ISS using the slime mold, Dictyostelium discoideum could potentially be performed in the flight verified hardware of the EMCS ARC Seed Cassettes.

biocompatibility↗

NASA NTRS2015

Halite as a Methane Sequestration Host: A Possible Explanation for Periodic Methane Release on Mars, and a Surface-accessible Source of Ancient Martian Carbon

We present the hypothesis that halite may play a role in methane sequestration on the martian surface. In terrestrial examples, halite deposits sequester large volumes of methane and chloromethane. Also, examples of chloromethane-bearing, approximately 4.5 Ga old halite from the Monahans meteorite show that this system is very stable unless the halite is damaged. On Mars, methane may be generated from carbonaceous material trapped in ancient halite deposits and sequestered. The methane may be released by damaging its halite host; either by aqueous alteration, aeolian abrasion, heating, or impact shock. Such a scenario may help to explain the appearance of short-lived releases of methane on the martian surface. The methane may be of either biogenic or abiogenic origin. If this scenario plays a significant role on Mars, then martian halite deposits may contain samples of organic compounds dating to the ancient desiccation of the planet, accessible at the surface for future sample return missions.

Fries, M. D.↗

NASA NTRS2012

Survival of B. Horneckiae Spores Under Ground-simulated Space Conditions

To prevent forward contamination and maintain the scientific integrity of future life detection missions, it is important to characterize and attempt to eliminate terrestrial microorganisms associated with exploratory spacecraft and landing vehicles. Among the organisms isolated from spacecraft-associated habitats, spore-forming microbes are highly resistant to various physical and chemical conditions, which include ionizing and UV radiation, desiccation and oxidative stress, and the harsh environment of outer space or planetary surfaces. Recently a radiation resistant, spore forming bacterial isolate, Bacillus horneckiae, was isolated from a clean room of the Kennedy Space Center where the Phoenix spacecraft was assembled. The exceptionally high tolerance of extreme conditions demonstrated by sporeforming bacteria highlighted the need to assess the viability of these microbes in situ (in real) space. The proposed BOSS (Biofilm Organisms Surfing Space) project aims to understand the mechanisms by which biofilm forming organisms, such as B. horneckiae, will potentially be able to withstand harsh space conditions. As previously stated, the spore producing ability of these species gives them increased survivability to harsh conditions. Some of the spores will have the protective exosporium layer artificially removed before the test to determine if the existence of this layer significantly changes the survivability during the mission. In preparation for that experiment, we analyzed spores which were exposed during a ground simulation, the EXPOSE R2 Biofilm Organisms Surfing Space (BOSS). Previous to exposure, spores were deposited onto spacecraft grade aluminum coupons in a spore suspension calculated to contain between 10(exp 7) and 10(exp 8) spores. This precursor series will be used to establish a baseline survivability function for comparison with the future flight tests during EXPOSE-R. For each coupon, a 10% polyvinyl alcohol (PVA) film was applied and peeled from the coupon to recover the spores. One hundred μl of sterile 10% PVA was applied to the surface of the coupon and allowed to dry for 1 hour at 37 C. The films were then removed using sterile scalpel and forceps and placed into a glass test tube containing 2 milliliters of sterile deionized water. The PVA film process was then repeated on each coupon one additional time to ensure recovery of the majority of spores. The second PVA film was added in the same glass tube as in the previous round. If the spores remained 100% viable, the test tubes should now contain between 5 X 10(exp 6) and 5 X 10(exp 7) spores per millimeter; however, it is expected that some loss of viability has occurred. In order to assess this loss, the number of colony forming, viable spores was counted. To count the colony forming units (CFUs), the spore containing solution was diluted in a process of 10-fold serial dilution by mixing successive solutions in a 100 microliter spore suspension to 900 microliter deionized H2O ratio. A sample dilution series revealed that 10(exp -3) and 10(exp -4) concentrations would be necessary for an accurate CFU count to be taken. For those two concentrations, a spread on a TSA plate was prepared and incubated at 32 C. For the samples exposed to UV radiation, the cell survivability was too low to establish a count from 100 microliter spread plating. Instead, no dilutions were performed and the entire 2 milliliter spore suspension was plated and incubated at 32 C. The plate's CFU counts were taken at 24 hours and 48 hours from the time of plating. At the end of the CFU counting the total surviving spores in each sample were calculated based on the number of CFUs that were observed per 100 microliters, or per 2 milliliters for the UV irradiated samples. The results of these calculations are shown in Figures 1 and 2.

cleanroom↗

NASA NTRS2015

Innovative Air Conditioning and Climate Control

NASA needed to develop a desiccant wheel based humidity removal system to enable the long term testing of the Orion CO2 scrubber on the International Space Station. In the course of developing that system, we learned three things that are relevant to energy efficient air conditioning of office towers. NASA developed a conceptual design for a humidity removal system for an office tower environment. We are looking for interested partners to prototype and field test this concept.

Graf, John↗

NASA NTRS2015

Computer Simulation and Modeling of CO2 Removal Systems for Exploration 2013-2014

The Atmosphere Revitalization Recovery and Environmental Monitoring (ARREM) project was initiated in September of 2011 as part of the Advanced Exploration Systems (AES) program. Under the ARREM project and the follow-on Life Support Systems (LSS) project, testing of sub-scale and full-scale systems has been combined with multiphysics computer simulations for evaluation and optimization of subsystem approaches. In particular, this paper will describes the testing and 1-D modeling of the combined water desiccant and carbon dioxide sorbent subsystems of the carbon dioxide removal assembly (CDRA). The goal is a full system predictive model of CDRA to guide system optimization and development.

Coker, R.↗

NASA NTRS2015

Life Beyond the Planet of Origin and Implications for the Search for Life on Mars

Outer space is vast, cold, devoid of matter, radiation filled with essentially no gravity. These factors present an environmental challenge for any form of life. Earth's biosphere has evolved for more than 3 billion years shielded from the hostile environment of outer space by the protective blanket of the atmosphere and magnetosphere. Space is a nutritional wasteland with no liquid water and readily available organic carbon. Moving beyond a life's planet of origin requires a means for transport, the ability to withstand transport, and the ability to colonize, thrive and ultimately evolve in the new environment. Can life survive beyond its home planet? The key to answering this question is to identify organisms that first have the ability to withstand space radiation, space vacuum desiccation and time in transit, and second the ability to grow in an alien environment. Within the last 60 years space technology allowed us to transport life beyond Earth's protective shield so we may study, in situ, their responses to selected conditions of space. To date a variety of microbes ranging from viruses, to Bacteria, to Archaea, to Eukarya have been tested in the space environment. Most died instantly, but not all. These studies revealed that ultraviolet radiation is the near-term lethal agent, while hard radiation is the long-term lethal agent when the organism is shielded from ultraviolet radiation. In fact, bacterial spores, halophilic cyanobacteria and Archaea as well as some lichens survive very well if protected from ultraviolet radiation [1]. Some microbes, then, may be able to survive the trip in outer space to Mars on a spacecraft or in a meteorite. Once on Mars can a terrestrial microbe survive? Although the conditions on Mars are not as harsh as those in space, they are not hospitable for a terrestrial microbe. Studies, however, have shown that certain microbes that can survive in space for several years may also be able to survive on Mars if protected from ultraviolet radiation [1]. Laboratory simulation experiments using a mock-up of the Phoenix lander have shown that microbes transported to the surface of Mars on a spacecraft come off the spacecraft and mix into the Martian regolith [2]. Additionally, studies simulating Martian dust storms demonstrate that microbes can survive in the Martian wind blown dust and be scattered across the Martian surface away from the spacecraft. Would these microbes that may survive on Mars metabolize and propagate? Growth requires liquid water, a carbon source and an energy source. Survival on Mars also requires protection from ultraviolet radiation. In the cold, dry environment of Mars the probability of microbial metabolism and growth at or just beneath the surface is extremely low. Although the probability is low, Mars may be contaminated with potentially live terrestrial organisms. In light of that statistic we must be extremely diligent and cautious in our search for Martian life. If we are not cautious we may find life on Mars and it may be a contaminant from Earth.

Survival in Space↗