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At least 163 records · Page 9

Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis

Abstract Protein activity, abundance, and stability can be regulated by post-translational modification including ubiquitination. Ubiquitination is conserved among eukaryotes and plays a central role in modulating cellular function; yet, we lack comprehensive catalogs of proteins that are modified by ubiquitin in plants. In this study, we describe an antibody-based approach to enrich ubiquitinated peptides coupled with isobaric labeling to enable quantification of up to 18-multiplexed samples. This approach identified 17,940 ubiquitinated lysine sites arising from 6,453 proteins from Arabidopsis (Arabidopsis thaliana) primary roots, seedlings, and rosette leaves. Gene ontology analysis indicated that ubiquitinated proteins are associated with numerous biological processes including hormone signaling, plant defense, protein homeostasis, and metabolism. We determined ubiquitinated lysine residues that directly regulate the stability of three transcription factors, CRYPTOCHROME-INTERACTING BASIC-HELIX-LOOP-HELIX 1 (CIB1), CIB1 LIKE PROTEIN 2 (CIL2), and SENSITIVE TO PROTON RHIZOTOXICITY1 (STOP1) using in vivo degradation assays. Furthermore, codon mutation of CIB1 to create a K166R conversion to prevent ubiquitination, via CRISPR/Cas9-derived adenosine base editing, led to an early flowering phenotype and increased expression of FLOWERING LOCUS T (FT). These comprehensive site-level ubiquitinome profiles provide a wealth of data for future functional studies related to modulation of biological processes mediated by this post-translational modification in plants.

Biochemistry & Molecular Biology↗

Model-independent determination of the axial mass parameter in quasielastic neutrino-nucleon scattering

Quasielastic neutrino-nucleon scattering is a basic signal process for neutrino oscillation studies. At accelerator energies, the corresponding cross section is subject to significant uncertainty due to the poorly constrained axial-vector form factor of the nucleon. A model-independent description of the axial-vector form factor is presented. Data from the MiniBooNE experiment for quasielastic neutrino scattering on {sup 12}C are analyzed under the assumption of a definite nuclear model. The value of the axial mass parameter, m{sub A}=0.85{sub -0.07}{sup +0.22}{+-}0.09 GeV, is found to differ significantly from extractions based on traditional form factor models. Implications for future neutrino scattering and pion electroproduction measurements are discussed.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

{pi}N transition distribution amplitudes: Their symmetries and constraints from chiral dynamics

Baryon to meson transition distribution amplitudes (TDAs) extend the concept of generalized parton distributions. Baryon to meson TDAs appear as building blocks in the collinear factorized description of amplitudes for a class of hard exclusive reactions, prominent examples of which being hard exclusive meson electroproduction off a nucleon in the backward region and baryon-antibaryon annihilation into a meson and a lepton pair. We study the general properties of these objects following the underlying symmetries of QCD. In particular, the Lorentz symmetry results in the polynomiality property of the Mellin moments in longitudinal momentum fractions. We present a detailed account of the isotopic and permutation symmetry properties of nucleon to pion ({pi}N) TDAs. This restricts the number of independent leading twist {pi}N TDAs to eight functions, providing description of all isotopic channels. Using chiral symmetry and the crossing relation between {pi}N TDAs and {pi}N generalized distribution amplitudes, we establish soft pion theorems for {pi}N TDAs, which determine the magnitude of {pi}N TDAs. Finally, we build a simple resonance exchange model for {pi}N TDAs considering N and {Delta}(1232) exchange contributions into the isospin-(1/2) and isospin-(3/2) {pi}N TDAs.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Z{gamma}{gamma} production with leptonic decays and triple photon production at next-to-leading order QCD

We present a calculation of the O({alpha}{sub s}) QCD corrections to the production of a Z boson in association with two photons and to triple-photon production at hadron colliders. All final-state photons are taken as real. For the Z boson, we consider the decays both into charged leptons and into neutrinos including all off-shell effects. Numerical results are obtained via a Monte Carlo program based on the structure of the VBFNLO program package. This allows us to implement general cuts and distributions of the final-state particles. We find that the next-to-leading order QCD corrections are sizable and significantly exceed the expectations from a scale variation of the leading-order result. In addition, differential distributions of important observables change considerably. The prediction of two-photon-associated Z production with Z decays into neutrinos from the charged-lepton rate works well, once we use an additional cut on the invariant mass of the charged-lepton pair.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Charm quark system at the physical point of 2+1 flavor lattice QCD

We investigate the charm quark system using the relativistic heavy quark action on 2+1 flavor PACS-CS configurations previously generated on 32{sup 3}x64 lattice. The dynamical up, down, and strange quark masses are set to the physical values by using the technique of reweighting to shift the quark-hopping parameters from the values employed in the configuration generation. At the physical point, the lattice spacing equals a{sup -1}=2.194(10) GeV and the spatial extent L=2.88(1) fm. The charm quark mass is determined by the spin-averaged mass of the 1S charmonium state, from which we obtain m{sub charm}{sup MS}({mu}=m{sub charm}{sup MS})=1.260(1)(6)(35) GeV, where the errors are due to our statistics, scale determination and renormalization factor. An additional systematic error from the heavy quark is of order {alpha}{sub s}{sup 2}f(m{sub Q}a)(a{Lambda}{sub QCD}), f(m{sub Q}a)(a{Lambda}{sub QCD}){sup 2}, which are estimated to be a percent level if the factor f(m{sub Q}a) analytic in m{sub Q}a is of order unity. Our results for the charmed and charmed-strange meson decay constants are f{sub D}=226(6)(1)(5) MeV, f{sub D{sub s}}=257(2)(1)(5) MeV, again up to the heavy quark errors of order {alpha}{sub s}{sup 2}f(m{sub Q}a)(a{Lambda}{sub QCD}), f(m{sub Q}a)(a{Lambda}{sub QCD}){sup 2}. Combined with the CLEO values for the leptonic decay widths, these values yield |V{sub cd}|=0.205(6)(1)(5)(9), |V{sub cs}|=1.00(1)(1)(3)(3), where the last error is because of the experimental uncertainty of the decay widths.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

LHC diphoton Higgs signal predicted by little Higgs models

Little Higgs theory naturally predicts a light Higgs boson whose most important discovery channel at the LHC is the diphoton signal pp{yields}h{yields}{gamma}{gamma}. In this work, we perform a comparative study for this signal in some typical little Higgs models, namely, the littlest Higgs model, two littlest Higgs models with T-parity (named LHT-I and LHT-II), and the simplest little Higgs models. We find that compared with the standard model prediction, the diphoton signal rate is always suppressed and the suppression extent can be quite different for different models. The suppression is mild (< or approx. 10%) in the littlest Higgs model but can be quite severe ({approx_equal}90%) in other three models. This means that discovering the light Higgs boson predicted by the little Higgs theory through the diphoton channel at the LHC will be more difficult than discovering the standard model Higgs boson.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

High-energy photon-hadron scattering in holographic QCD

This article provides an in-depth look at hadron high-energy scattering by using gravity dual descriptions of strongly coupled gauge theories. Just like deeply inelastic scattering (DIS) and deeply virtual Compton scattering (DVCS) serve as clean experimental probes into nonperturbative internal structure of hadrons, elastic scattering amplitude of a hadron and a (virtual) photon in gravity dual can be exploited as a theoretical probe. Since the scattering amplitude at sufficiently high energy (small Bjorken x) is dominated by parton contributions (=Pomeron contributions) even in strong coupling regime, there is a chance to learn a lesson for generalized parton distribution (GPD) by using gravity dual models. We begin with refining derivation of the Brower-Polchinski-Strassler-Tan (BPST) Pomeron kernel in gravity dual, paying particular attention to the role played by the complex spin variable j. The BPST Pomeron on warped spacetime consists of a Kaluza-Klein tower of 4D Pomerons with nonlinear trajectories, and we clarify the relation between Pomeron couplings and the Pomeron form factor. We emphasize that the saddle-point value j* of the scattering amplitude in the complex j-plane representation is a very important concept in understanding qualitative behavior of the scattering amplitude. The total Pomeron contribution to the scattering is decomposed into the saddle-point contribution and at most a finite number of pole contributions, and when the pole contributions are absent (which we call saddle-point phase), kinematical variable (q,x,t)-dependence of ln(1/q) evolution and ln(1/x) evolution parameters {gamma}{sub eff} and {lambda}{sub eff} in DIS and t-slope parameter B of DVCS in HERA experiment are all reproduced qualitatively in gravity dual. All of these observations shed a new light on modeling of GPD. Straightforward application of those results to other hadron high-energy scattering is also discussed.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Hyperon forward spin polarizabilty {gamma}{sub 0}

We present the results of a systematic leading-order calculation of hyperon Compton scattering and extract the forward spin polarizability--{gamma}{sub 0}--of hyperons within the framework of SU(3) heavy baryon chiral perturbation theory (HBChPT). The results obtained for {gamma}{sub 0} in the case of nucleons agree with that of the known results of SU(2) HBChPT when kaon loops are not considered.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Cosmogenic gamma rays and the composition of cosmic rays

We discuss the prospects of detecting the sources of ultrahigh energy (UHE) cosmic ray (CR) nuclei via their emission of cosmogenic {gamma} rays in the GeV to TeV energy range. These {gamma} rays result from electromagnetic cascades initiated by high energy photons, electrons, and positrons that are emitted by CRs during their propagation in the cosmic radiation background and are independent of the simultaneous emission of {gamma} rays in the vicinity of the source. The corresponding production power by UHE CR nuclei (with mass number A and charge Z) is dominated by pion photo production ({proportional_to}A) and Bethe-Heitler pair production ({proportional_to}Z{sup 2}). We show that the cosmogenic {gamma}-ray signal from a single steady UHE CR source is typically more robust with respect to variations of the source composition and injection spectrum than the accompanying signal of cosmogenic neutrinos. We study the diffuse emission from the sum of extragalactic CR sources as well as the point-source emission of the closest sources.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Consequences of neutrino Lorentz violation for leptonic meson decays

If the observation by OPERA of apparently superluminal neutrinos is correct, the Lagrangian for second-generation leptons must break Lorentz invariance. We calculate the effects of an energy-independent change in the neutrino speed on another observable, the charged pion decay rate. The rate decreases by a factor [1-(3/1-m{sub {mu}}{sup 2}/m{sub {pi}}{sup 2})(<v{sub {nu}}>-1)], where <v{sub {nu}}> is the (directionally averaged) neutrino speed in the pion's rest frame. This provides a completely independent experimental observable that is sensitive to the same forms of Lorentz violation as a neutrino time-of-flight measurement.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Associated Higgs-W-Boson Production at Hadron Colliders: A Fully Exclusive QCD Calculation at NNLO

We consider QCD radiative corrections to standard model Higgs-boson production in association with a W boson in hadron collisions. We present a fully exclusive calculation up to next-to-next-to-leading order (NNLO) in QCD perturbation theory. To perform this NNLO computation, we use a recently proposed version of the subtraction formalism. Our calculation includes finite-width effects, the leptonic decay of the W boson with its spin correlations, and the decay of the Higgs boson into a bb pair. We present selected numerical results at the Tevatron and the LHC.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Probing Nonrelativistic QCD Factorization in Polarized J/{psi} Photoproduction at Next-to-Leading Order

We analyze the polarization observables of J/{psi} photoproduction at next-to-leading order within the factorization formalism of nonrelativistic quantum chromodynamics (NRQCD). This is a complete next-to-leading-order study of heavy-quarkonium polarization including the full relativistic corrections due to the intermediate {sup 1}S{sub 0}{sup [8]}, {sup 3}S{sub 1}{sup [8]}, and {sup 3}P{sub J}{sup [8]} color-octet states closing a gap in the worldwide endeavor to test NRQCD factorization at the quantum level. We present theoretical predictions in the helicity, target, and Collins-Soper frames of DESY HERA, evaluated using the color-octet long-distance matrix elements previously extracted through a global fit to experimental data of unpolarized J/{psi} production, and confront them with recent measurements by the H1 and ZEUS Collaborations. We find the overall agreement to be satisfactory but the case for NRQCD to be not as strong as for the J/{psi} yield.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Implementation and Validation of Photon Transport in OpenMC

OpenMC is a Monte Carlo particle transport code focused on reactor physics calculations. It stochastically simulates neutrons moving through 3D models using constructive solid geometry and continuous-energy cross sections. Recently, a photon transport capability was added to OpenMC. In reactor simulations, photon transport is needed, for example, to account for photon heating: although the majority of the energy deposited at a fission site comes from the kinetic energy of the fission fragments, a nontrivial portion can be carried away by photons. Therefore, photons produced in neutron reactions must be simulated in order to accurately model energy deposition. OpenMC can now simulate neutron-induced photon production as well as perform pure photon calculations. The four basic photon interactions with matter — coherent (Rayleigh) scattering, incoherent (Compton) scattering, the photoelectric effect, and pair production — are modeled. Secondary processes that can create new photons (i.e., atomic relaxation, electron-positron annihilation, and bremsstrahlung) are also included. The methods implemented here were validated against MCNP6 for a variety of materials and energies.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗