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At least 163 records · Page 9

PPI DataHub Project Data Package: S. elongatus PCC 7942 Circadian Control Bioproduction Metabolomics (PB-DP5)

The purpose of this experiment was to evaluate how circadian clock regulation impacts carbon partitioning between storage, growth, and product synthesis in Synechococcus elongatus PCC 7942 in providing insights to strategies for enhanced bioproduction. Culture samples were collected at 0, 0.5, 1, 2, 4, 6, and 8 hours for extracellular sucrose analysis. Circadian metabolomics data was acquired using a Agilent single quadrupole gas chromatography-mass spectrometer and processed using Agilent Mass Hunter for targeted sucrose quantification. Metabolomic analysis of PCC 7942 light-dark cycle cultures transitioned to constant light revealed distinct temporal patterns in sucrose production. Processed metabolomic datasets are openly accessible from the PNNL DataHub project dataset download page and contain secondary processed GC-MS results files and supporting metadata materials linked to relevant source code information supporting data transparency and reuse.

59 BASIC BIOLOGICAL SCIENCES↗

Increasing the Scale of the Mass Spectrometry Query Language Compendium with Explainable AI

A significant bottleneck in metabolomics data interpretation is the effective use of domain knowledge to assign structural information based on fragmentation patterns. The mass spectrometry query language (MassQL) aims to make this process accessible and applicable across multiple analysis platforms. While advanced computational methods are capable of predicting compound structures from fragmentation data, AI/ML approaches often rely on complex, opaque criteria that are difficult to interpret or modify. As a result, their predictive patterns cannot be readily translated into human-readable rules, such as those used in MassQL. Here, in this study, we introduce ChemEcho, a machine learning embedding method that converts tandem mass spectrometry data into sparse feature vectors containing peak and neutral mass subformulae to enhance explainable AI/ML-based methods. An advantage of this approach is that decision trees trained using these feature vectors can be directly translated to MassQL. Using a battery of decision trees trained using ChemEcho embeddings to predict molecular attributes, we generated over 1500 MassQL queries for 765 molecular features and evaluated their precision and recall. From these queries, the 50 highest-performing queries were integrated into the MassQL compendium. This set of generated MassQL queries included environmentally and biologically relevant classes such as PFAS and molecules containing phosphate or sulfate substructures. To illustrate the impact these queries would have on a typical metabolomics experiment, these MassQL queries were applied to a public metabolomics data set─resulting in a marked increase in the structural information derived from tandem mass spectra. Access and reuse of these queries is expected to enhance structural annotation in untargeted experiments, leading to more specific claims and advancing many applications in metabolomics.

Harwood, Thomas V. [USDOE Joint Genome Institute (↗

Plant Nutrition Influences Resistant Maize Defense Responses to the Fall Armyworm ( Spodoptera frugiperda )

Plants are often confronted by different groups of herbivores, which threaten their growth and reproduction. However, they are capable of mounting defenses against would-be attackers which may be heightened upon attack. Resistance to insects often varies among plant species, with different genotypes exhibiting unique patterns of chemical and physical defenses. Within this framework, plant access to nutrients may be critical for maximal functioning of resistance mechanisms and are likely to differ among plant genotypes. In this study, we aimed to test the hypothesis that access to nutrition would alter the expression of plant resistance to insects and alter insect performance in a manner consistent with fertilization regime. We used two maize (Zea mays) genotypes possessing different levels of resistance and the fall armyworm (Spodoptera frugiperda) as model systems. Plants were subjected to three fertilization regimes prior to assessing insect-mediated responses. Upon reaching V4 stage, maize plants were separated into two groups, one of which was infested with fall armyworm larvae to induce plant defenses. Plant tissue was collected and used in insect bioassays and to measure the expression of defense-related genes and proteins. Insect performance differed between the two plant genotypes substantially. For each genotype, fertilization altered larval performance, where lower fertilization rates hindered larval growth. Induction of plant defenses by prior herbivory substantially reduced naïve fall armyworm growth in both genotypes. The effects between fertilization and induced defenses were complex, with low fertilization reducing induced defenses in the resistant maize. Gene and protein expression patterns differed between the genotypes, with herbivory often increasing expression, but differing between fertilization levels. The soluble protein concentrations did not change across fertilization levels but was higher in the susceptible maize genotype. These results demonstrate the malleability of plant defenses and the cascading effects of plant nutrition on insect herbivory.

54 ENVIRONMENTAL SCIENCES↗

Electrical Evaluation of RCA MWS5501D Random Access Memory, Volume 2, Appendix a

The electrical characterization and qualification test results are presented for the RCA MWS5001D random access memory. The tests included functional tests, AC and DC parametric tests, AC parametric worst-case pattern selection test, determination of worst-case transition for setup and hold times, and a series of schmoo plots. The address access time, address readout time, the data hold time, and the data setup time are some of the results surveyed.

Klute, A.↗

Survey on Efficient and Productive Use of Electricity In Women-Run Small Businesses in Uganda

In Uganda, women make up a large portion of the service sector, notably in informal small and micro businesses such as retail shops, food preparation, tailoring, sewing, beer brewing, basket weaving, healthcare, and hairdressing. Lawrence Berkeley National Laboratory (LBNL), and USAID, in partnership with the Clean Energy Enthusiasts (CEE), a local implementer in Uganda, designed and conducted a participatory survey to collect baseline data and information on the current state of benefits to energy access. The study included data on the uptake of efficient and productive electric (EPUE) equipment, business skills, and financial needs among small and mid-size women-led businesses in Uganda. The objective of the survey was to build evidence and identify economic activities where access to electricity has a positive impact on women’s empowerment by helping them develop new and existing businesses, improve productivity, and increase their income. The survey included questions about the type of electric equipment used and needed, electric consumption patterns, alternative sources to electricity, and business and capital barriers related to increased access. The survey results provided the basis for designing a training program tailored to the need of women entrepreneurs in the region.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Imaging conical intersection dynamics during azobenzene photoisomerization by ultrafast X-ray diffraction

Significance Vibronic coherences are unique features that emerge in the decisive moments of photochemistry and photophysics. Here we demonstrate how X-ray diffraction can access fundamental information about these coherences. This is enabled by recent developments at free-electron lasers, working toward temporal resolution and single-molecule accessibility of scattering-based measurements. Time-resolved diffraction patterns of the isomerization of azobenzene are simulated. The process is monitored in time via the momentum-space signal, with a special focus on the mixed elastic/inelastic scattering from coherences. We give practical ideas on how this relatively weak contribution to the signal can potentially be retrieved. A direct connection to the real-space movie of the conical intersection dynamics is made, enabling the observation of quantum coherences that direct chemical processes.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Fpack and Funpack Utilities for FITS Image Compression and Uncompression

Fpack is a utility program for optimally compressing images in the FITS (Flexible Image Transport System) data format (see http://fits.gsfc.nasa.gov). The associated funpack program restores the compressed image file back to its original state (as long as a lossless compression algorithm is used). These programs may be run from the host operating system command line and are analogous to the gzip and gunzip utility programs except that they are optimized for FITS format images and offer a wider choice of compression algorithms. Fpack stores the compressed image using the FITS tiled image compression convention (see http://fits.gsfc.nasa.gov/fits_registry.html). Under this convention, the image is first divided into a user-configurable grid of rectangular tiles, and then each tile is individually compressed and stored in a variable-length array column in a FITS binary table. By default, fpack usually adopts a row-by-row tiling pattern. The FITS image header keywords remain uncompressed for fast access by FITS reading and writing software. The tiled image compression convention can in principle support any number of different compression algorithms. The fpack and funpack utilities call on routines in the CFITSIO library (http://hesarc.gsfc.nasa.gov/fitsio) to perform the actual compression and uncompression of the FITS images, which currently supports the GZIP, Rice, H-compress, and PLIO IRAF pixel list compression algorithms.

Pence, W.↗

"Giovanni at 20: Consistency and Persistency in Making Earth Remote Sensing Data Available (and Useful) to the Earth Science Community"

Since its creation in the year 2000, the NASA Geospatial Interactive Online Visualization ANd aNalysis Infrastructure (Giovanni) has been an exemplar of how to provide remotely-sensed satellite data and related Earth science datasets to a broad and globally diverse researcher community. One of the hallmarks of Giovanni system usage is that it is common to see practitioners of many branches of science – particularly those in fields not traditionally associated with remote-sensing data, such as animal behavior and paleooceanography – both accessing and employing datasets which the system provides. This usage pattern is attested to by the diversity of published science citing Giovanni. Giovanni has evolved through several versions, each of which increased analytical and visualization options while also enhancing ease-of-use. Information resources supporting Giovanni from the Goddard Earth Sciences Data and Information Services Center (GES DISC), which hosts the system, start with basic mapping and plotting functions and extend to “How-to” recipes demonstrating interusability with other data analysis systems and software. Giovanni is now being developed for use in a cloud environment, potentially expanding datasets the system can be applied to, and also improving performance for data having high spatial and temporal resolution. This presentation covers the system’s historical success with interesting examples of Giovanni-citing research, and will apply its current capabilities to a multi-dataset examination of derecho events associated with mesoscale convective phenomena in the summer of 2020, indicating how Giovanni is prepared for ongoing support of Earth science in a changing world.

Giovanni Derecho visualization↗

Global patterns in cloud forms on Mars

Mariner 9 images and all Viking orbiter images through July 1979 were searched for cloud forms. A computer-accessible catalog was assembled, consisting of a classification of cloud type (lee wave, for example) and properties (directionality, wavelength, for example). Lee wave directionality shows a pattern and seasonal variation at high latitudes which is consistent with predictions of theoretical modeling. Fog and haze occurrence shows no obvious correlation with water abundance or any other simple causal factor. Lee waves are rare at equatorial latitudes. Plumes (probably dust) occur preferentially at locations where strong boundary layer convection is expected.

French, R. G.↗

Exploring Nonmotorized Travel in New York State Using 2017 National Household Travel Survey Data

This report presents systematic analysis of nonmotorized travel behavior within New York State (NYS) utilizing 2017 National Household Travel Survey data. As walking and biking assume increasingly prominent roles in advancing active transportation objectives, accessible mobility, and sustainable transportation systems, comprehensive understanding of these modal patterns becomes essential for evidence-based policy development and strategic planning initiatives. Recognizing the substantial geographic, demographic, and socioeconomic heterogeneity characterizing NYS—spanning from the concentrated urban fabric of New York City (NYC) to dispersed suburban and rural contexts—this analysis employs the structured "4Ws" analytical framework to examine participant demographics, spatial and temporal distributions, and motivational factors underlying active transportation choices relative to motorized alternatives. This methodological approach captures modal behavior patterns, user characteristics, trip purposes, and temporal variations across the state's diverse contexts. Additionally, the research examines behavioral differences among distinct user classifications, including walk-only and bike-only travelers, while conducting comparative analysis of nonmotorized travel patterns between ALICE (Asset Limited, Income Constrained, Employed) and non-ALICE household categories.

99 GENERAL AND MISCELLANEOUS↗

Extending the utility of forest cover maps

A computer-assisted map analysis system is discussed that characterizes timber supply in terms of standing timber; accessibility considering various harvesting factors; and availability as affected by ownership patterns of timberlands. Factors affecting harvesting include proximity to existing roads and consideration of terrain characteristics. Availability considerations include size of ownership, housing density and excluded areas. The model is demonstrated for a seventy square mile tract in western Massachusetts.

Berry, J. K.↗

Near-field testing of the 30 GHz TRW proof-of-concept multibeam antenna

Near-field testing was conducted on the 30 GHz TRW proof-of-concept (POC) Multibeam Antenna (MBA). The TRW POC MBA is a dual offset Cassegrain reflector system using a 2.7 m main reflector. This configuration was selected to assess the ability to create both multiple fixed and scanned spot beams. The POC configuration investigated frequency reuse via spatial separation of beams, polarization selectivity and time division multiple access scanning at 30 GHz. Measurements of directivity, sidelobe level, and pattern were made at NASA Lewis Research Center's Near-Field Antenna Test Facility. Presented in this paper are complete results of these measurements. Included is a detailed discussion of all testing procedures and parameters. Results of additional testing used to evaluate diffraction effects of the subreflector and distortions of the main reflector are also presented.

Kunath, R. R., Jr.↗

Table look-up approach to pattern recognition.

The table look-up approach is based on prestoring in fast, random-access, core memory the desired answer (e.g., crop-type) for all combinations of multispectral scanner outputs from selected channels. Specifically, each set of measurements from a given point on the ground is interpreted as that address in core memory where the answer can be retrieved. Substituting the simple retrieval operation for the length y computations required by the conventional approach offers two advantages: (1) the processing time is reduced by more than an order of magnitude; (2) the multispectral scanner data can be processed by computers having minimal sophistication, complexity, and cost. These two advantages may make it possible to use an onboard computer to perform the classification function in flight.

Eppler, W. G.↗

ISS Wastewater Pretreatment via DNA Pattern Picofilter Using Inorganic Brine Stimulant

Astronauts in the International Space Station (ISS) need as much water as they can access, whether it comes from people's breath, sweat or urine, recycled shower water or hand washing. It is of vital importance that any type of water is recycled and filtrated through different methods so it can be re-used. Recently, Cerahelix Inc. offered a ceramic tubular membrane element that uses DNA strands as a pattern in a sol-gel process that allows the sintered product to have a pore size in the picometer scale and claims to achieve ten times higher purity than other commercially available ceramic filters. This should allow higher purities and yields at reduced energy costs and theoretically allow near total dewatering of the reject stream and greater than 80% removal of polyvalent ions. Trials were performed with Cerahelix Inc. filters using an inorganic brine simulant at two pHs to test the efficiency of Pico Helix TM membranes and determine their feasibility for spacecraft wastewater treatment processes. Emphasis was placed on the polyvalent ions: SO42-, PO43-, Mg2+, and Ca2+. These ions should be rejected almost entirely from the feed solution and, not permeate through the filter. Results show that at a pH of 4.2, permeation of the polyvalent ions varied from 59% to 74% and at pH of 8.0, permeation varied from 72% to 87%. An Extended Nernst Planck (ENP) approach that describes the mass transfer process for a pico-filtration membrane was used to construct a model to inform the experimental expectations and outcome.

Carolina Franco↗

Results From Invoking Artificial Neural Networks to Measure Insider Threat Detection & Mitigation

Advances on differentiating between malicious intent and natural “organizational evolution” to explain observed anomalies in operational workplace patterns suggest benefit from evaluating collective behaviors observed in the facilities to improve insider threat detection and mitigation (ITDM). Advances in artificial neural networks (ANN) provide more robust pathways for capturing, analyzing, and collating disparate data signals into quantitative descriptions of operational workplace patterns. In response, a joint study by Sandia National Laboratories and the University of Texas at Austin explored the effectiveness of commercial artificial neural network (ANN) software to improve ITDM. Overall, this research demonstrates the benefit of learning patterns of organizational behaviors, detecting off-normal (or anomalous) deviations from these patterns, and alerting when certain types, frequencies, or quantities of deviations emerge for improving ITDM. Evaluating nearly 33,000 access control data points and over 1,600 intrusion sensor data points collected over a nearly twelve-month period, this study's results demonstrated the ANN could recognize operational patterns at the Nuclear Engineering Teaching Laboratory (NETL) and detect off-normal behaviors—suggesting that ANNs can be used to support a data-analytic approach to ITDM. Several representative experiments were conducted to further evaluate these conclusions, with the resultant insights supporting collective behavior-based analytical approaches to quantitatively describe insider threat detection and mitigation.

45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEF↗

Dual-Wavelength Simultaneous Patterning of Degradable Thermoset Supports for One-Pot Embedded 3D Printing

Vat photopolymerization (VP) techniques have enabled the fabrication of complex geometries while balancing high precision and fast processing times. 3D printed objects are traditionally built layer-by-layer with newly cured layers being structurally supported by previous ones. Fabricating unsupported features such as overhangs and arches risks misalignment and sagging, limiting the range of accessible designs. To overcome this issue, support structures are fabricated along with the primary object as temporary scaffolds that provide stability and conserve print fidelity. For VP specifically, patterning dissolvable sacrificial supports is attractive to avoid manual removal after printing. In this study, we demonstrate a base-degradable thermoset to pattern print supports in a one-pot formulation along with the primary structural material. Efficient printing is enabled using a dual-wavelength negative imaging (DWNI) DLP printer that patterns the degradable thermoset with visible light and the permanent network with UV light, which are simultaneously projected using a single digital micromirror device (DMD). Printed objects undergo thermal postprocessing to enhance the final conversion of the primary material, after which thermoset supports are degraded in a basic, aqueous solution. This approach provides a robust method for the dual-wavelength patterning of sacrificial thermoset supports, broadening the range of accessible 3D printable materials and geometries.

3D printing↗

Hourly Load Profile Dataset for Electric Port Cargo Handling Equipment in the United States

Historically, ports have relied on fossil fuels, particularly diesel, as their primary energy source. Transitioning to electric cargo handling equipment (eCHE) offers a promising solution, as this relatively mature technology eliminates tailpipe emissions, reduces harmful airborne particulates, lowers noise pollution, and supports decarbonization. This study develops an initial estimation of hourly electricity demand for eCHE at the top 25 container ports (by tonnage) in the United States. These datasets, accessible at data.nrel.gov/submissions/281, provide valuable insights into the electricity demand patterns and potential grid impacts associated with widespread eCHE adoption, forming a foundation for future refinement based on stakeholder feedback.

33 ADVANCED PROPULSION SYSTEMS↗

Full color hybrid display for aircraft simulators

A full spectrum color monitor, connected to the camera and lens system of a television camera supported by a gantry frame over a terrain model simulating an aircraft landing zone, projects the monitor image onto a lens or screen visually accessible to a trainee in the simulator. A digital computer produces a pattern corresponding to the lights associated with the landing strip onto a monochromatic display, and an optical system projects the calligraphic image onto the same lens so that it is superposed on the video representation of the landing field. The optical system includes a four-color wheel which is rotated between the calligraphic display and the lens, and an apparatus for synchronizing the generation of a calligraphic pattern with the color segments on the color wheel. A servo feedback system responsive to the servo motors on the gantry frame produces an input to the computer so that the calligraphically generated signal corresponds in shape, size and location to the video signal.

Chase, W. D.↗