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162 records · Page 9
All sourcesNASA NTRSDOE OSTI
NASA NTRS2001

Relationship between radiation-induced aberrations in individual chromosomes and their DNA content: effects of interaction distance

PURPOSE: To study the effect of the interaction distance on the frequency of inter- and intrachromosome exchanges in individual chromosomes with respect to their DNA content. Assumptions: Chromosome exchanges are formed by misrejoining of two DNA double-strand breaks (DSB) induced within an interaction distance, d. It is assumed that chromosomes in G(0)/G(1) phase of the cell cycle occupy a spherical domain in a cell nucleus, with no spatial overlap between individual chromosome domains. RESULTS: Formulae are derived for the probability of formation of inter-, as well as intra-, chromosome exchanges relating to the DNA content of the chromosome for a given interaction distance. For interaction distances <1 microm, the relative frequency of interchromosome exchanges predicted by the present model is similar to that by Cigarran et al. (1998) based on the assumption that the probability of interchromosome exchanges is proportional to the "surface area" of the chromosome territory. The "surface area" assumption is shown to be a limiting case of d-->0 in the present model. The present model also predicts that the probability of intrachromosome exchanges occurring in individual chromosomes is proportional to their DNA content with correction terms. CONCLUSION: When the interaction distance is small, the "surface area" distribution for chromosome participation in interchromosome exchanges has been expected. However, the present model shows that for the interaction distance as large as 1 microm, the predicted probability of interchromosome exchange formation is still close to the surface area distribution. Therefore, this distribution does not necessarily rule out the formation of complex chromosomal aberrations by long-range misrejoining of DSB.

Non-programmatic↗
NASA NTRS2021

LUVOIR-ECLIPS Closed-Loop Adaptive Optics Performance and Contrast Predictions

One of the primary science goals of the Large UV/Optical/Infrared Surveyor (LUVOIR) mission concept is to detect and characterize Earth-like exoplanets orbiting nearby stars with direct imaging. The success of its coronagraph instrument ECLIPS (Extreme Coronagraph for Living Planetary Systems) depends on the ability to stabilize the wavefront from a large segmented mirror such that optical path differences are limited to tens of picometers RMS during an exposure time of a few hours. In order to relax the constraints on the mechanical stability, ECLIPS will be equipped with a wavefront sensing and control (WS\&C) architecture to correct wavefront errors up to temporal frequencies $\gtrsim$1 Hz. These errors may be dominated by spacecraft structural dynamics exciting vibrations at the segmented primary mirror. In this work, we present detailed simulations of the WS\&C system within the ECLIPS instrument and the resulting contrast performance. This study assumes wavefront aberrations based on a finite element model of a simulated telescope with spacecraft structural dynamics. Wavefront residuals are then computed according to a model of the adaptive optics system that includes numerical propagation to simulate a realistic wavefront sensor and an analytical model of the temporal performance. An end-to-end numerical propagation model of ECLIPS is then used to estimate the residual starlight intensity distribution at the science detector. We show that the contrast performance depends strongly on the target star magnitude and the spatio-temporal distribution of wavefront errors from the telescope. In cases with significant vibration, we advocate for the use of laser metrology to mitigate high temporal frequency wavefront errors and increase the mission yield.

Sirbu, Dan↗
NASA NTRS1998

Instrument for Achieving High Angular Resolution on the Infrared Telescope

Aberrations in stellar images caused by the atmosphere sets a significant limit on angular resolution in ground based astronomy. The largest of these aberrations is the image motion or wavefront tilt. Since the image motion is random it causes a blurring of the image, and this causes a blurring of the image from 0.3 arcseconds to about 0.7 arcseconds. The purpose of the tip-tilt project was to devise a system for the NASA Infrared Telescope Facility that would measure the image movement and correct it by rapidly tilting a mirror in two axes (tip and tilt). The system would involve building a CCD sensor package to measure the image motion, a new top end for the telescope to hold the tip-tilt mirror, a control system, and software. The system was designed to correct images for the facility camera, NSFCAM, and for the facility spectrometer, SPEX. Both of these instruments are equipped with a cold beamsplitter to feed the sensor package.

Hall, Donald N. B.↗
NASA NTRS1994

In-orbit performance of the COSTAR-corrected Faint Object Camera

The improvements due to the Corrective Optics Space Telescope Axial Replacement (COSTAR) on imaging with the Faint Object Camera (FOC) on board the Hubble Space Telescope (HST) are presented. The encircled energy performance is dramatically improved, such that 85% of the total light in the Point Spread Function (PSF) is now enclosed within a circle of radius 0.1 sec at 486 nm wavelength, compared to 18% in the spherically aberrated PSF. This is equivalent to a sensitivity increase of 1.6 mag. The effective angular resolution is also improved from 66 to 43 mas at 486 nm. These improvements are slightly offset by a 20% lower total throughput at visible wavelengths. The plate scale is changed from 22.3 mas/pixel to 14.35 mas/pixel, resulting in a decrease in the field of view from 11 x 11/sq arcsec to 7.3 x 7.3/sq arcsec for the workhorse 512 x 512 format.

Jedrzejewski, R. I.↗
NASA NTRS1997

Results of the Compensated Earth-Moon-Earth Retroreflector Laser Link (CEMERLL) Experiment

Adaptive optics techniques can be used to realize a robust low bit-error-rate link by mitigating the atmosphere-induced signal fades in optical communications links between ground-based transmitters and deep-space probes. Phase I of the Compensated Earth-Moon-Earth Retroreflector Laser Link (CEMERLL) experiment demonstrated the first propagation of an atmosphere-compensated laser beam to the lunar retroreflectors. A 1.06-micron Nd:YAG laser beam was propagated through the full aperture of the 1.5-m telescope at the Starfire Optical Range (SOR), Kirtland Air Force Base, New Mexico, to the Apollo 15 retroreflector array at Hadley Rille. Laser guide-star adaptive optics were used to compensate turbulence-induced aberrations across the transmitter's 1.5-m aperture. A 3.5-m telescope, also located at the SOR, was used as a receiver for detecting the return signals. JPL-supplied Chebyshev polynomials of the retroreflector locations were used to develop tracking algorithms for the telescopes. At times we observed in excess of 100 photons returned from a single pulse when the outgoing beam from the 1.5-m telescope was corrected by the adaptive optics system. No returns were detected when the outgoing beam was uncompensated. The experiment was conducted from March through September 1994, during the first or last quarter of the Moon.

Wilson, K. E.↗
NASA NTRS1994

Imaging the effect of dipole tilt on magetotail boundaries

Eight years of Interplanetary Monitoring Platform (IMP) 8, four years of International Sun Earth Explorer (ISEE) 2, and one year of IMP 7 magnetometer data have been combined to produce an 'image' of the average magnetic field for a YZ cross section (aberrated GSM) of the magnetotail at a downtail distance of 25 R(sub E). The shape of the neutral sheet and magnetopause boundaries can be observed directly from the images. A fitting function that qualitatively matches the observed boundary shape can then be chosen. This approach improves on previous fits to possible unsuitable functional forms specified independently of the data. In addition, as a refinement of previous studies, we have corrected for varying solar wind dynamic pressure and the effects of tail flaring. We find the magnetopause is displaced above the XY plane with increasing dipole tilt. The neutral sheet is found to curve slightly more than the model of Fairlfield (1980) during times of large dipole tilt and near the flanks appears to differ substantially from the neutral sheet shape given by the analytic model of Voigt (1984), the more recent neutral sheet model of Dandouras (1988) based on the Voigt model, and the semi-empirical model of Tsyganenko (1989).

Hammond, C. Max↗
NASA NTRS2003

A Theoretical Light Scattering Model of Nanoparticle Laser Tweezers

Accomplishments this reporting period include: 1. derived, programmed, checked, and tested the Mie light scattering theory formulas for the radiation trapping force for both the on-axis and off-axis geometry of the trapping beam plus trapped spherical particle; 2. verified that the computed radiation trapping force for a freely propagating focused Gaussian laser beam incident on a spherical particle agrees with previous published calculations; 3. compared the small particle size and large particle size limits of the Mie calculation with the results of Rayleigh scattering theory and ray scattering theory, respectively and verified that the comparison is correct for Rayleigh scattering theory but found that ray theory omits an important light scattering effect included in the Mie theory treatment; 4. generalized the calculation of the radiation trapping force on a spherical particle in the on-axis geometry from a freely propagating focused Gaussian laser beam to the realistic situation of a Gaussian beam truncated and focused by a high numerical aperture oil-immersion microscope objective lens and aberrated by the interface between the microscope cover slip and the liquid-filled sample volume; and 5. compared the calculated radiation trapping force for this geometry with the results of previously published experiments and found that the agreement is better than when using previously developed theories.

Lock, James A.↗
NASA NTRS1994

FOC SV support and science data analysis

The main activity of NASA grant NAG5-1733 has been the study of Planetary Nebulae in the Magellanic Clouds through observations using the ESA Faint Object Camera conducted during Hubble Space Telescope (HST) Observing Cycles 1 and 2 in the pre-Costar era and in Cycle 4 with Costar and the FOC. A table of the observations obtained during these observing periods is attached along with figures showing the final images. Both the table and the figures are intended for publication later this year in the Astrophysical journal. Work that had been carried out under this grant included: astrometric measurement of ground-based images and preparation of detailed observing proposals; re-calibration of FOC images; deconvolution; and data analysis and measurement. The work has resulted in two papers, the first already published and the second in preparation by Blades, Osmer and Barlow. A reprint of the first paper is attached. The second paper will enlarge upon the results described in the first paper, bearing in mind the much larger sample of objects. Finally, the recently acquired images in Cycle 4 with the corrected optics will allow us to make direct and quantitative comparison between the pre- and post-correction for FOC. This work will be carried out under a separate grant. All the observations concerning Planetary Nebulae as described in an earlier status report have been collected and measured. In addition, a preliminary study of the photometric response of the FOC was begun under this grant but not completed because the analysis of the Cycle 2 Planetary Nebulae took much longer and was much more difficult than originally envisaged (due to the spherical aberration problem). However, because our data covers a long time period, we shall be able to deduce the photometric properties now using the actual PN themselves. This work will be carried out under a separate grant.

Blades, J. Chris↗
NASA NTRS1992

Iterative deconvolution of x ray and optical SNR images

Blind Iterative Deconvolution (BID) is a technique which was originally developed to correct the degrading effects of atmospheric turbulence on astronomical images from single short exposure, high signal-to-noise-ratio frames. At the Center for Astro physics, we have implemented a version of BID following the general approach of Ayers and Dainty (1988), but extending the technique to use Wiener filtering, and developed it for application to high energy images from Einstein and ROSAT. In the optical, the point spread function (PSF) that degrades the images is due to a combination of telescope and atmospheric aberrations. At high energies, the degrading function is the instrument response function, which is known to be time and energy level unstable. In both cases, the PSF is poorly known, so BID can be used to extract the PSF from the image and then deconvolve the blurred image to produce a sharpened image. Other aspects of this technique are discussed.

Nisenson, Peter↗
NASA NTRS2001

Probabilities of radiation-induced inter- and intrachromosomal exchanges and their dependence on the DNA content of the chromosome

A biophysical model has been developed that is based on the assumptions that an interphase chromosome occupies a spherical territory and that chromosome exchanges are formed by the misrejoining of two DNA double-strand breaks induced within a defined interaction distance. The model is used to explain the relative frequencies of inter- and intrachromosomal exchanges and the relationship between radiation-induced aberrations in individual chromosomes and the DNA content of the chromosome. Although this simple model predicts a higher ratio of inter- to intrachromosomal exchanges for low-LET radiation than for high-LET radiation, as has been suggested by others, we argue that the comparison of the prediction of the model with experimental results is not straightforward. With the model, we also show that the probability of the formation of interchromosomal exchanges is proportional to the "surface area" of the chromosome domain plus a correction term. The correction term is small if the interaction distance is less than 1 microm for both low- and high-LET radiations.

NASA Center JSC↗
NASA NTRS1992

Radiative equilibrium in rapidly expanding shells

The equation of radiative equilibrium for rapidly expanding spherical shells including the special relativistic terms is discussed, and a temperature-correction procedure for the solution of this equation is given. The temperature-correction scheme combines a partial linearization approach with the approximate Lambda-operator iteration method. For some test problems, the temperature structures of atmospheres in radiative equilibrium in the Lagrangian frame are calculated. The effects of the velocity field on the temperature stratification and the luminosity are also discussed. We find that the temperature structure of a moving photosphere in radiative equilibrium in the Lagrangian frame differs significantly from that of a static photosphere and that, at least, all first-order terms, in particular the advection and aberration terms, have to be included consistently both in the radiative transfer and the energy equation in studies of supernovae atmospheres and other high-speed radiating flows.

Hauschildt, P. H.↗
NASA NTRS2008

Optical Modeling Activities for NASA's James Webb Space Telescope (JWST): V. Operational Alignment Updates

This paper is part five of a series on the ongoing optical modeling activities for the James Webb Space Telescope (JWST). The first two papers discussed modeling JWST on-orbit performance using wavefront sensitivities to predict line of sight motion induced blur, and stability during thermal transients. The third paper investigates the aberrations resulting from alignment and figure compensation of the controllable degrees of freedom (primary and secondary mirrors), which may be encountered during ground alignment and on-orbit commissioning of the observatory, and the fourth introduced the software toolkits used to perform much of the optical analysis for JWST. The work here models observatory operations by simulating line-of-sight image motion and alignment drifts over a two-week period. Alignment updates are then simulated using wavefront sensing and control processes to calculate and perform the corrections. A single model environment in Matlab is used for evaluating the predicted performance of the observatory during these operations.

Howard, Joseph M.↗
NASA NTRS2002

Hypergravity-induced immunomodulation in a rodent model: lymphocytes and lymphoid organs

The major goal of this study was to quantify changes in lymphoid organs and cells over time due to centrifugation-induced hypergravity. C57BL/6 mice were exposed to 1, 2 and 3 G and the following assays were performed on days 1, 4, 7, 10, and 21: spleen, thymus, lung, and liver masses; total leukocyte, lymphocyte, monocyte/macrophage, and granulocyte counts; level of splenocyte apoptosis; enumeration of CD3+ T, CD3+/CD4+ T helper, CD3+/CD8+ T cytotoxic, B220+ B, and NK1.1+ natural killer cells; and quantification of cells expressing CD25, CD69, and CD71 activation markers. The data show that increased gravity resulted in decreased body, spleen, thymus, and liver, but not lung, mass. Significant reductions were noted in all three major leukocyte populations (lymphocytes, granulocytes, monocyte/macrophages) [correction of macrphages] with increased gravity; persistent depletion was noted in blood but not spleen. Among the various lymphocyte populations, the CD3+/CD8+ T cells and B220+ B cells were the most affected and NK1.1+ NK cells the least affected. Overall, the changes were most evident during the first week, with a greater influence noted for cells in the spleen. A linear relationship was found between some of the measurements and the level of gravity, especially on day 4. These findings indicate that hypergravity profoundly alters leukocyte number and distribution in a mammalian model and that some aberrations persisted throughout the three weeks of the study. In certain cases, the detected changes were similar to those observed after whole-body irradiation. In future investigations we hope to combine hypergravity with low-dose rate irradiation and immune challenge.

Non-NASA Center↗
NASA NTRS1982

Cooled grating array spectrometer for 0.6-5 microns

A grating spectrometer, designed to illuminate an array of 122 InSb photodiodes with minimum aberrations and maximum speed, has been constructed. The instrument will be used on the 5 meter Hale telescope at Palomar Observatory, and is easily adaptable to telescopes of various focal ratios. A resolving power of 100-1000 can be obtained at wavelengths between 0.6 microns and 5 microns with remotely interchangeable gratings. The spectrometer is sufficiently compact to fit on the 8-inch work surface of a commercially available dewar, and uses simple on-axis spherical and paraboloidal optical elements. The camera mirror produces an f/2.5 beam which, with the 0.2 mm detectors, allows a 3-in. focal-plane aperture on the 5 meter telescope. All rays fall within a 100 microns blur circle at all points along the array. Distortions have been corrected with a tilted field flattening lens in front of the detector.

Nordholt, J. E.↗
NASA NTRS2011

Phase Retrieval for Radio Telescope and Antenna Control

Phase-retrieval is a general term used in optics to describe the estimation of optical imperfections or "aberrations." The purpose of this innovation is to develop the application of phase retrieval to radio telescope and antenna control in the millimeter wave band. Earlier techniques do not approximate the incoherent subtraction process as a coherent propagation. This approximation reduces the noise in the data and allows a straightforward application of conventional phase retrieval techniques for radio telescope and antenna control. The application of iterative-transform phase retrieval to radio telescope and antenna control is made by approximating the incoherent subtraction process as a coherent propagation. Thus, for systems utilizing both positive and negative polarity feeds, this approximation allows both surface and alignment errors to be assessed without the use of additional hardware or laser metrology. Knowledge of the antenna surface profile allows errors to be corrected at a given surface temperature and observing angle. In addition to imperfections of the antenna surface figure, the misalignment of multiple antennas operating in unison can reduce or degrade the signal-to-noise ratio of the received or broadcast signals. This technique also has application to the alignment of antenna array configurations.

Dean, Bruce↗
NASA NTRS2001

Adjustable Focus Optical Correction Lens (AFOCL)

This report describes the activities and accomplishments along with the status of the characterization of a PLZT-based Adjustable Focus Optical Correction Lens (AFOCL) test device. The activities described in this report were undertaken by members of the Center for Applied Optics (CAO) at the University of Alabama in Huntsville (UAH) under NASA Contract NAS8-00188. The effort was led by Dr. Bruce Peters as the Principal Investigator and supported by Dr. Patrick Reardon, Ms. Deborah Bailey, and graduate student Mr. Jeremy Wong. The activities outlined for the first year of the contract were to identify vendors and procure a test device along with performing the initial optical characterization of the test device. This activity has been successfully executed and test results are available and preliminary information was published at the SPIE Photonics West Conference in San Jose, January 2001. The paper, "Preliminary investigation of an active PLZT lens," was well received and generated response with several questions from the audience. A PLZT test device has been commercially procured from an outside vendor: The University of California in San Diego (UCSD) in partnership with New Interconnect Packaging Technologies (NIPT) Inc. The device has been subjected to several tests to characterize the optical performance of the device at wavelengths of interest. The goal was to evaluate the AFOCL similar to a conventional lens and measure any optical aberrations present due to the PLZT material as a deviation in the size of the diffraction limited spot (blur), the presence of diffracted energy into higher orders surrounding the focused spot (a variation in Strehl), and/or a variation or spread in the location of the focused energy away from the optical axis (a bias towards optical wedge, spherical, comma, or other higher order aberrations). While data has been collected indicative of the imaging quality of the AFOCL test device, it was not possible to fully characterize the optical performance of the AFOCL alone because there were significant optical distortions due to fabrication related issues.

Peters, Bruce R.↗
NASA NTRS2009

Probing Millisecond Pulsar Emission Geometry Using Light Curves From the Fermi Large Area Telescope

An interesting new high-energy pulsar sub-population is emerging following early discoveries of gamma-ray millisecond pulsars (MSPs) by the Fermi Large Area Telescope (LAT). We present results from 3D emission modeling, including the Special Relativistic effects of aberration and time-of-flight delays and also rotational sweepback of 13-field lines, in the geometric context of polar cap (PC), slot gap (SG), outer gap (OG), and two-pole caustic (TPC) pulsar models. In contrast to the general belief that these very old, rapidly-rotating neutron stars (NSs) should have largely pair-starved magnetospheres due to the absence of significant pair production, we find that most of the light curves are best fit by SG and OG models, which indicates the presence of narrow accelerating gaps limited by robust pair production -- even in these pulsars with very low spin-down luminosities. The gamma-ray pulse shapes and relative phase lags with respect to the radio pulses point to high-altitude emission being dominant for all geometries. We also find exclusive differentiation of the current gamma-ray MSP population into two MSP sub-classes: light curve shapes and lags across wavebands impose either pair-starved PC (PSPC) or SG / OG-type geometries. In the first case, the radio pulse has a small lag with respect to the single gamma-ray pulse, while the (first) gamma-ray peak usually trails the radio by a large phase offset in the latter case. Finally, we find that the flux correction factor as a function of magnetic inclination and observer angles is typically of order unity for all models. Our calculation of light curves and flux correction factor f(_, _, P) for the case of MSPs is therefore complementary to the "ATLAS paper" of Watters et al. for younger pulsars.

Venter, Christo↗
NASA NTRS2001

Physical and biological studies with protons and HZE particles in a NASA supported research center in radiation health

NASA has established and supports a specialized center for research and training (NSCORT) to specifically address the potential deleterious effects of HZE particles on human health. The NSCORT in radiation health is a joint effort between Lawrence Berkeley National Laboratory (LBNL) and Colorado State University (CSU). The overall scope of research encompasses a broad range of subjects from microdosimetric studies to cellular and tissue responses to initial damage produced by highly energetic protons and heavy charged particles of the type found in galactic cosmic rays (GCR) spectrum. The objectives of the microdosimetry studies are to determine the response of Tissue Equivalent Proportional Counter (TEPC) to cosmic rays using ground based accelerators. This includes evaluation of energy loss due to the escape of high-energy delta rays and increased energy deposition due to the enhanced delta ray production in the wall of the detector. In this report major results are presented for 56Fe at 1000, 740, 600 and 400 MeV/nucleon. An assessment of DNA repair and early development of related chromosomal changes is extremely important to our overall understanding of enhanced biological effectiveness of high LET particle radiation. Results are presented with respect to the fidelity of the rejoining of double strand breaks and the implications of misrejoining. The relationship between molecular and cytogenetic measurements is presented by studying damage processing in highly heterochromatic supernumerary (correction of sypernumerary) X chromosomes and the active X-chromosome. One of the important consequences of cell's inability to handle DNA damage can be evaluated through mutation studies. Part of our goal is the assessment of potential radioprotectors to reduce the mutation yield following HZE exposures, and some promising results are presented on one compound. A second goal is the integration of DNA repair and mutation studies. Results are presented on a direct comparison of initial double strand breaks induction, the time course and fidelity of double strand break rejoining, cell killing and mutation induction in the same human model system. In order to understand the carcinogenic potential of protons and HZE particles, the role of damaged microenvironment in this process must be understood. In this project it has been postulated that radiation affects the microenvironment, which then modifies cell interactions in a manner conducive to neoplastic progression. Both TGF-beta and FGF-2 are important components of microenvironment. A recent result on the assessment of the role of FGF-2 and its cross-talk with TGF-beta as a function of radiation quality is presented. Theoretical modeling has so far played a central role in analyzing and integrating experimental data on repair and mutation studies and predicting new phenomena. The integrated NSCORT program also provides a broad training experience for students and postdoctoral fellows in space radiation health.

NASA Discipline Radiation Health↗