Structural Adaptation in Its Orphan Domain Engenders Betaglycan with an Alternate Mode of Growth Factor Binding Relative to Endoglin
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Utilities are proposing changes to residential rate structures to address concerns about lost revenue due to increased adoption of distributed solar generation. An investigation of the impacts of increased fixed charges, minimum bills and residential demand charges on PV and non-PV customer bills suggests that minimum bills more accurately capture utilities' revenue requirement than fixed charges, while not acting as a disincentive to efficiency or negatively impacting low-income customers.
Agent-based transportation models are critical tool for evaluating energy use, emissions and mobility impacts of transportation. In this context, vehicle ownership models are critical for linking agent activities to their impacts on the transportation system. However, no agent-based freight transportation models to date has been developed that predicts the fuel technology that is preferred by establishments when purchasing a Light Duty Vehicle (LDV) for business purposes. This research addresses this gap by using recently collected survey data to estimate a discrete choice model that can be applied in the agent-based context.
Highlights: • Application of LCA in planning integrated waste management systems. • Environmental valuation of 3 legacy scenarios for the Olympic Park. • Hot-spot analysis highlights the importance of energy and materials recovery. • Most environmental savings are achieved through materials recycling. • Sensitivity analysis shows importance of waste composition and recycling rates. - Abstract: This paper presents the results of the life cycle assessment (LCA) of 10 integrated waste management systems (IWMSs) for 3 potential post-event site design scenarios of the London Olympic Park. The aim of the LCA study is to evaluate direct and indirect emissions resulting from various treatment options of municipal solid waste (MSW) annually generated on site together with avoided emissions resulting from energy, materials and nutrients recovery. IWMSs are modelled using GaBi v6.0 Product Sustainability software and results are presented based on the CML (v.Nov-10) characterisation method. The results show that IWMSs with advanced thermal treatment (ATT) and incineration with energy recovery have the lowest Global Warming Potential (GWP) than IWMSs where landfill is the primary waste treatment process. This is due to higher direct emissions and lower avoided emissions from the landfill process compared to the emissions from the thermal treatment processes. LCA results demonstrate that significant environmental savings are achieved through substitution of virgin materials with recycled ones. The results of the sensitivity analysis carried out for IWMS 1 shows that increasing recycling rate by 5%, 10% and 15% compared to the baseline scenario can reduce GWP by 8%, 17% and 25% respectively. Sensitivity analysis also shows how changes in waste composition affect the overall result of the system. The outcomes of such assessments provide decision-makers with fundamental information regarding the environmental impacts of different waste treatment options necessary for sustainable waste management planning.
Highlights: • Little is known about FEW impacts of managing food waste after it has been disposed. • Food waste management options have variable FEW impacts and opportunities. • Preventable and unpreventable food waste have different mechanisms of FEW impact. • A “food-waste-FEW systems” approach may maximize benefits across FEW sectors. • Characterizing FEW nexus impacts of wasted food is a priority for future work. - Abstract: Throughout the world, much food produced is wasted. The resource impact of producing wasted food is substantial; however, little is known about the energy and water consumed in managing food waste after it has been disposed. Herein, we characterize food waste within the Food-Energy-Water (FEW) nexus and parse the differential FEW effects of producing uneaten food and managing food loss and waste. We find that various food waste management options, such as waste prevention, landfilling, composting, anaerobic digestion, and incineration, present variable pathways for FEW impacts and opportunities. Furthermore, comprehensive sustainable management of food waste will involve varied mechanisms and actors at multiple levels of governance and at the level of individual consumers. To address the complex food waste problem, we therefore propose a “food-waste-systems” approach to optimize resources within the FEW nexus. Such a framework may be applied to devise strategies that, for instance, minimize the amount of edible food that is wasted, foster efficient use of energy and water in the food production process, and simultaneously reduce pollution externalities and create opportunities from recycled energy and nutrients. Characterization of FEW nexus impacts of wasted food, including descriptions of dynamic feedback behaviors, presents a significant research gap and a priority for future work. Large-scale decision making requires more complete understanding of food waste and its management within the FEW nexus, particularly regarding post-disposal impacts related to water.
Highlights: • A group of microRNAs contributes to the regulation of SRSF1, SRSF2 and hnRNP A1. • microRNAs targeting SRSF1 and SRSF2 are involved in a regulatory feedback loop. • microRNAs that regulate SRSF2 affect also the expression of apoptotic genes. • 3′UTR variants of SRSF1 and SRSF2 differ by the presence of miRNA-binding sites. SRSF1, SRSF2 and hnRNP A1 are splicing factors that regulate the expression of oncogenes and tumor suppressors. SRSF1 and SRSF2 contribute to the carcinogenesis in the kidney. Despite their importance, the mechanisms regulating their expression in cancer are not entirely understood. Here, we investigated the microRNA-mediated regulation of SRSF1, SRSF2 and hnRNP A1 in renal cancer. The expression of microRNAs predicted to target SRSF1, SRSF2 and hnRNP A1 was disturbed in renal tumors compared with controls. Using qPCR, Western blot/ICC and luciferase reporter system assays we identified microRNAs that contribute to the regulation of expression of SRSF1 (miR-10b-5p, miR-203a-3p), SRSF2 (miR-183-5p, miR-200c-3p), and hnRNP A1 (miR-135a-5p, miR-149-5p). Silencing of SRSF1 and SRSF2 enhanced the expression of their targeting microRNAs. miR-183-5p and miR-200c-3p affected the expression of SRSF2-target genes, TNFRSF1B, TNFRSF9, CRADD and TP53. 3′UTR variants of SRSF1 and SRSF2 differed by the presence of miRNA-binding sites. In conclusion, we identified a group of microRNAs that contribute to the regulation of expression of SRSF1, SRSF2 and hnRNP A1. The microRNAs targeting SRSF1 and SRSF2 are involved in a regulatory feedback loop. microRNAs miR-183-5p and miR-200c-3p that target SRSF2, affect the expression of genes involved in apoptotic regulation.
Dendritic cells are one of the most popular immune cells, which plays a remarkable role in both immunotherapy and tolerance induction. Due to unwanted side effects of leukocyte presence in donated blood, the policy of blood service is the pre-storage reduction of leukocytes, which today, filtration is the most common method for this purpose. The filtration method has led to diminished access to Buffy coat as a generally used conventional source of biological cells. We developed a simple, affordable, and reproducible method for dendritic cell differentiation from filter-derived monocytes and, the results of the filter study were compared with differentiated DCs from the conventional buffy coat-derived monocytes. The Monocytes were recovered from leukoreduction filter using an optimized protocol with supplemented PBS buffer. Following the adhesion method, CD14{sup +} Monocyte-enriched population with the purity of 94 % was obtained. After cytokine stimulation over a 6-day period and maturation induction by LPS, differentiated DCs were evaluated for morphology, surface markers (CD86, CD40, CD83 and, HLA-DR), antigen uptake potency and IL-12 secretion. Analysis and comparison of the results represented no significant difference between the two groups. Accordingly, we conclude that leukoreduction filters could be introduced as a reliable and research-grade source of monocyte for DC generation in biological research.
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In this study, carbon dioxide is one of the end products of combustion, and is not a benign component of the atmosphere. The concentration of CO 2 in the atmosphere has reached unprecedented levels and continues to increase owing to an escalating rate of fossil fuel combustion, causing concern about climate change and rising sea levels. In view of the inevitable depletion of fossil fuels, a possible solution to this problem is the recycling of carbon dioxide, possibly captured at its point of generation, to fuels. Researchers in this field are using solar energy for CO 2 activation and utilization in several ways: (i) so-called artificial photosynthesis using photo-induced electrons; (ii) bulk electrolysis of a CO 2 saturated solution using electricity produced by photovoltaics; (iii) CO 2 hydrogenation using solar-produced H 2 ; and (iv) the thermochemical reaction of metal oxides at extremely high temperature reached by solar collectors. Since the thermodynamics of CO 2 at high temperature (> 1000 ºC) are quite different from those near room temperature, only chemistry below 200 ºC is discussed in this review.
Cellulosic biomass-based sustainable aviation fuels (SAFs) can be produced from various feedstocks. The breakeven price and carbon intensity of these feedstock-to-SAF pathways are likely to differ across feedstocks and across spatial locations due to differences in feedstock attributes, productivity, opportunity costs of land for feedstock production, soil carbon effects, and feedstock composition. We integrate feedstock to fuel supply chain economics and life-cycle carbon accounting using the same system boundary to quantify and compare the spatially varying greenhouse gas (GHG) intensities and costs of GHG abatement with SAFs derived from four feedstocks (switchgrass, miscanthus, energy sorghum, and corn stover) at 4 km resolution across the U.S. rainfed region. We show that the optimal feedstock for each location differs depending on whether the incentive is to lower breakeven price, carbon intensity, or cost of carbon abatement with biomass or to have high biomass production per unit land. The cost of abating GHG emissions with SAF ranges from $\$181$ Mg –1 CO 2 e to more than $\$444$ Mg –1 CO 2 e and is lowest with miscanthus in the Midwest, switchgrass in the south, and energy sorghum in a relatively small region in the Great Plains. While corn stover-based SAF has the lowest breakeven price per gallon, it has the highest cost of abatement due to its relatively high GHG intensity. Furthermore, our findings imply that different types of policies, such as volumetric targets, tax credits, and low carbon fuel standards, will differ in the mix of feedstocks they incentivize and locations where they are produced in the U.S. rainfed region.
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