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At least 1,603 records · Page 89

Overcoming Barriers in Plant Transformation: A Focus on Bioenergy Crops

Although 2023 marked the 40th anniversary of the first transgenic plant, routine transformation of most plant genotypes remains elusive. Rapid systems to overexpress, interfere, or knock out genes— collectively defined in this report as “transformation and editing technologies”—are needed to understand plant gene function. This understanding in turn is crucial for efficiently developing new, sustainable, high-yielding, and climate-resilient crops to meet the growing demand for food, feed, fiber, and fuel. In particular, the ability to apply transformation and editing technologies to bioenergy crops has remained largely unrealized. To address this opportunity, the U.S. Department of Energy (DOE) Biological and Environmental Research Program convened a workshop on September 18–20, 2023, to define transformation and editing needs and barriers focused on bioenergy crops. The main conclusions are summarized below.

09 BIOMASS FUELS↗

Diversification of aminoacyl-tRNA synthetase activities via genomic duplication

Intricate evolutionary events enabled the emergence of the full set of aminoacyl-tRNA synthetase (aaRS) families that define the genetic code. The diversification of aaRSs has continued in organisms from all domains of life, yielding aaRSs with unique characteristics as well as aaRS-like proteins with innovative functions outside translation. Recent bioinformatic analyses have revealed the extensive occurrence and phylogenetic diversity of aaRS gene duplication involving every synthetase family. However, only a fraction of these duplicated genes has been characterized, leaving many with biological functions yet to be discovered. Here we discuss how genomic duplication is associated with the occurrence of novel aaRSs and aaRS-like proteins that provide adaptive advantages to their hosts. We illustrate the variety of activities that have evolved from the primordial aaRS catalytic sites. This precedent underscores the need to investigate currently unexplored aaRS genomic duplications as they may hold a key to the discovery of exciting biological processes, new drug targets, important bioactive molecules, and tools for synthetic biology applications.

noncanonical functions↗

Explaining the stellar initial mass function with the theory of spatial networks

The distributions of stars and prestellar cores by mass (initial and dense core mass functions, IMF/DCMF) are among the key factors regulating star formation and are the subject of detailed theoretical and observational studies. Results from numerical simulations of star formation qualitatively resemble an observed mass function, a scale-free power law with a sharp decline at low masses. However, most analytic IMF theories critically depend on the empirically chosen input spectrum of mass fluctuations which evolve into dense cores and, subsequently, stars, and on the scaling relation between the amplitude and mass of a fluctuation. Here we propose a new approach exploiting techniques from the field of network science. We represent a system of dense cores accreting gas from the surrounding diffuse interstellar medium (ISM) as a spatial network growing by preferential attachment and assume that the ISM density has a self-similar fractal distribution following the Kolmogorov turbulence theory. We effectively combine gravoturbulent and competitive accretion approaches and predict the accretion rate to be proportional to the dense core mass: dM/dt∝M. Then we describe the dense core growth and demonstrate that the power-law core mass function emerges independently of the initial distribution of density fluctuations by mass. Our model yields a power law solely defined by the fractal dimensionalities of the ISM and accreting gas. With a proper choice of the low-mass cut-off, it reproduces observations over three decades in mass. We also rule out a low-mass star dominated “bottom-heavy” IMF in a single star-forming region.

79 ASTRONOMY AND ASTROPHYSICS↗

Prediction of the diffuse far-infrared flux from the galactic plane

A basic model and simple numerical relations useful for future far-infrared studies of the Galaxy are presented. Making use of recent CO and other galactic surveys, the diffuse far-infrared flux distribution from the galactic plane is predicted as a function of galactic longitude for longitudes between 4 and 90 deg; the far-infrared emissivity is predicted as a function of galactocentric distance. Future measurements of the galactic far-infrared flux would yield valuable information on the physical properties and distribution of dust and molecular clouds in the Galaxy, particularly the inner region.

Fazio, G. G.↗

Prediction of the diffuse far-infrared flux from the galactic plane

A basic model and simple numerical relations useful for future far-infrared studies of the Galaxy are presented. Making use of recent CO and other galactic surveys, the diffuse far-infrared flux distribution from the galactic planes is predicted as a function of galactic longitude for the region between 4 and 90 deg longitude; the far-infrared emissivity is predicted as a function of galactocentric distance. Future measurements of the galactic far-infrared flux would yield valuable information on the physical properties and distribution of dust and molecular clouds in the Galaxy, particularly the inner region.

Fazio, G. G.↗

Extinction-sedimentation inversion technique for measuring size distribution of artificial fogs

In measuring the size distribution of artificial fog particles, it is important that the natural state of the particles not be disturbed by the measuring device, such as occurs when samples are drawn through tubes. This paper describes a method for carrying out such a measurement by allowing the fog particles to settle in quiet air inside an enclosure through which traverses a parallel beam of light for measuring the optical depth as a function of time. An analytic function fit to the optical depth time decay curve can be directly inverted to yield the size distribution. Results of one such experiment performed on artificial fogs are shown as an example. The forwardscattering corrections to the measured extinction coefficient are also discussed with the aim of optimizing the experimental design so that the error due to forwardscattering is minimized.

Deepak, A.↗

Laboratory measurements of the cometary photochemical phenomena

Laboratory experiments are described that provide fundamental information about photochemical processes in comets. The yield of cometary radicals such as CN, OH, etc. can be determined as a function of photolyzing wavelength. Quantum state distributions of the internal energy of the cometary radicals can also be measured as a function of wavelength permitting one to define the recoil velocity of the fragments. This type of information supplies the data needed for more elaborate models to interpret the data being obtained on comets.

Jackson, W. M.↗

The energy separation between the classical and nonclassical isomers of protonated acetylene - An extensive study in one- and n-particle space saturation

The energy separation between the classical and nonclassical forms of protonated acetylene has been reinvestigated in light of the recent experimentally deduced lower bound to this value of 6.0 kcal/mol. The objective of the present study is to use state-of-the-art ab initio quantum mechanical methods to establish this energy difference to within chemical accuracy (i.e., about 1 kcal/mol). The one-particle basis sets include up to g-type functions and the electron correlation methods include single and double excitation coupled-cluster (CCSD), the CCSD(T) extension, multireference configuration interaction, and the averaged coupled-pair functional methods. A correction for zero-point vibrational energies has also been included, yielding a best estimate for the energy difference between the classical and nonclassical forms of 3.7 + or - 1.3 kcal/mol.

Lindh, Roland↗

Extrapolation procedures in Mott electron polarimetry

In standard Mott electron polarimetry using thin gold film targets, extrapolation procedures must be used to reduce the experimentally measured asymmetries A to the values they would have for scattering from single atoms. These extrapolations involve the dependent of A on either the gold film thickness or the maximum detected electron energy loss in the target. A concentric cylindrical-electrode Mott polarimeter, has been used to study and compare these two types of extrapolations over the electron energy range 20-100 keV. The potential systematic errors which can result from such procedures are analyzed in detail, particularly with regard to the use of various fitting functions in thickness extrapolations, and the failure of perfect energy-loss discrimination to yield accurate polarizations when thick foils are used.

Gay, T. J.↗

Electrolytic smelting of lunar rock for oxygen, iron, and silicon

Preliminary studies of the electrochemical properties of silicate melts such as those available from heating of lunar mare soils indicate that conductivities are high enough for design of a practical electrolytic cell. The nature and kinetics of the electrode reactions, which involve reduction of Fe(++) and Si(IV) and oxidation of silicate anions as the primary, product-forming reactions, are also satisfactory. A survey of the efficiencies for production (amount of product for a given current) of O2, Fe(sup 0), and Si(sup 0) as functions of potential and of electrolyte composition indicate that conditions can be chosen to yield high production efficiencies. We also conclude that electronic conductivity does not occur to a significant extent. Based on these data, a cell with electrodes of 30 sq m in area operating between 1 and 5V with a current between 1.6 and 3.5(10)(exp 5) A for a mean power requirement of 0.54 MW and total energy use of approximately 13 MWhr per 24-hr day would produce 1 ton of O2, 0.81 ton of Fe(sup 0), 0.65 ton of Si(sup 0) (as Fe(sup 0)-Si(sup 0) alloy), and about 3.5 tons of silicate melt of altered composition per 24 hr. Adjustable distance between electrodes could offer flexibility with respect to feedstock and power source.

Haskin, Larry A.↗

Nonlinear dynamics of charged particles in the magnetotail

An important region of the earth's magnetosphere is the nightside magnetotail, which is believed to play a significant role in energy storage and release associated with substorms. The magnetotail contains a current sheet which separates regions of oppositely directed magnetic field. Particle motion in the collisionless magnetotail has been a long-standing problem. Recent research from the dynamical point of view has yielded considerable new insights into the fundamental properties of orbits and of particle distribution functions. A new framework of understanding magnetospheric plasma properties is emerging. Some novel predictions based directly on nonlinear dynamics have proved to be robust and in apparent good agreement with observation. The earth's magnetotail may serve as a paradigm, one accessible by in situ observation, of a broad class of boundary regions with embedded current sheets. This article reviews the nonlinear dynamics of charged particles in the magnetotail configuration. The emphasis is on the relationships between the dynamics and physical observables. At the end of the introduction, sections containing basic material are indicated.

Chen, James↗

Compressibility effects on rotor forces in the leakage path between a shrouded pump impeller and its housing

A modified approach to Childs' previous work on fluid-structure interaction forces in the leakage path between an impeller shroud and its housing is presented in this paper. Three governing equations consisting of continuity, path-momentum, and circumferential-momentum equations were developed to describe the leakage path inside a pump impeller. Radial displacement perturbations were used to solve for radial and circumferential force coefficients. In addition, impeller-discharge pressure disturbances were used to obtain pressure oscillation responses due to precessing impeller pressure wave pattern. Childs' model was modified from an incompressible model to a compressible barotropic-fluid model (the density of the working fluid is a function of the pressure and a constant temperature only). Results obtained from this model yielded interaction forces for radial and circumferential force coefficients. Radial and circumferential forces define reaction forces within the impeller leakage path. An acoustic model for the same leakage path was also developed. The convective, Coriolis, and centrifugal acceleration terms are removed from the compressible model to obtain the acoustics model. A solution due to impeller discharge pressure disturbances model was also developed for the compressible and acoustics models. The results from these modifications are used to determine what effects additional perturbation terms in the compressible model have on the acoustic model. The results show that the additional fluid mechanics terms in the compressible model cause resonances (peaks) in the force coefficient response curves. However, these peaks only occurred at high values of inlet circumferential velocity ratios greater than 0.7. The peak pressure oscillation was shown to occur at the wearing ring seal. Introduction of impeller discharge disturbances with n = 11 diametral nodes showed that maximum peak pressure oscillations occurred at nondimensional precession frequencies of f = 6.4 and f = 7.8 for this particular pump. Bolleter's results suggest that for peak pressure oscillations to occur at the wearing ring seal, the nondimensional excitation frequency should be on the order of f = 2.182 for n = 11. The resonances found in this research do not match the excitation frequencies predicted by Bolleter. At the predicted peak excitation frequencies given by Bolleter, the compressible model shows an attenuation of the pressure oscillations at the seal exit. The compressibility of the fluid does not have a significant influence on the model at low values of nondimensional excitation frequency. At high values of nondimensional frequency, the effects of compressibility become more significant. For the acoustic analysis, the convective, Coriolis, and centrifugal acceleration terms do affect the results to a limited extent for precession excitation and to a large extent for a pressure excitation when the fluid operates at relatively high Mach numbers.

Cao, Nhai The↗

The proton cyclotron instability and the anisotropy/B inverse correlation

Spacecraft observations in the strongly compressed subsolar magnetosheath show an inverse correlation between the proton temperature anistrophy (T(sub perpendicular to p)/T(sub parallel to p) greater than 1 where perpendicular to and parallel to denote directions perpendicular and parallel to the background magnetic field) and the parallel proton beta (beta(sub parallel to p). This manuscript uses one-dimensional hybrid simulations of the proton cyclotron anisotropy instability in homogeneous electron-proton plasmas to study this correlation which may represent a limited closure relation for fluid theories of anisotropic space plasmas. The emphasis is on driven simulation which increase the temperature anisotropy by periodically reducing the magnetic-field-aligned velocities of the protons. The late-time states from ensembles of both initial value and driven simulations yield very similar expressions for the proton anisotropy/beta(sub parallel to P) inverse correlation, and provide a basis for explaining differences between sheath observations from different spacecraft. The driven simulations also yield expressions for the maximum instability growth rate and the fluctuating field energy as functions of beta (sub parallel to p) and a parameter characterizing the anisotropy driver.

Gary, S. Peter↗

The correlated k-distribution technique as applied to the AVHRR channels

Correlated k-distributions have been created to account for the molecular absorption found in the spectral ranges of the five Advanced Very High Resolution Radiometer (AVHRR) satellite channels. The production of the k-distributions was based upon an exponential-sum fitting of transmissions (ESFT) technique which was applied to reference line-by-line absorptance calculations. To account for the overlap of spectral features from different molecular species, the present routines made use of the multiplication transmissivity property which allows for considerable flexibility, especially when altering relative mixing ratios of the various molecular species. To determine the accuracy of the correlated k-distribution technique as compared to the line-by-line procedure, atmospheric flux and heating rate calculations were run for a wide variety of atmospheric conditions. For the atmospheric conditions taken into consideration, the correlated k-distribution technique has yielded results within about 0.5% for both the cases where the satellite spectral response functions were applied and where they were not. The correlated k-distribution's principal advantages is that it can be incorporated directly into multiple scattering routines that consider scattering as well as absorption by clouds and aerosol particles.

Kratz, David P.↗

Sputtering of Metals by Mass-Analyzed N2(+) and N(+)

Low-energy sputtering studies were conducted with the help of a specially designed ion accelerator. A high-intensity rf ion source was developed for use in conjunction with electrostatic acceleration and magnetic mass separation of ion beams in the 0 to 8 kev energy range. Beams of N(+) or N2(+) ions have been produced with intensities of 200 to 500 micro-a (approx. 1 sq cm in cross section) and energy half-widths of about 20 ev. The sputtering yields of five metals (Cu, Ni, Fe, Mo, and W) were obtained as a function of energy (0-8 kev), bombarding ion (N(+) and N2(+)), and angle of incidence (normal and 450). Results are presented and some of their theoretical implications are discussed.

Bader, Michel↗

First-Order Model Management With Variable-Fidelity Physics Applied to Multi-Element Airfoil Optimization

First-order approximation and model management is a methodology for a systematic use of variable-fidelity models or approximations in optimization. The intent of model management is to attain convergence to high-fidelity solutions with minimal expense in high-fidelity computations. The savings in terms of computationally intensive evaluations depends on the ability of the available lower-fidelity model or a suite of models to predict the improvement trends for the high-fidelity problem, Variable-fidelity models can be represented by data-fitting approximations, variable-resolution models. variable-convergence models. or variable physical fidelity models. The present work considers the use of variable-fidelity physics models. We demonstrate the performance of model management on an aerodynamic optimization of a multi-element airfoil designed to operate in the transonic regime. Reynolds-averaged Navier-Stokes equations represent the high-fidelity model, while the Euler equations represent the low-fidelity model. An unstructured mesh-based analysis code FUN2D evaluates functions and sensitivity derivatives for both models. Model management for the present demonstration problem yields fivefold savings in terms of high-fidelity evaluations compared to optimization done with high-fidelity computations alone.

Alexandrov, N. M.↗

Advanced Aerodynamic Design of Passive Porosity Control Effectors

This paper describes aerodynamic design work aimed at developing a passive porosity control effector system for a generic tailless fighter aircraft. As part of this work, a computational design tool was developed and used to layout passive porosity effector systems for longitudinal and lateral-directional control at a low-speed, high angle of attack condition. Aerodynamic analysis was conducted using the NASA Langley computational fluid dynamics code USM3D, in conjunction with a newly formulated surface boundary condition for passive porosity. Results indicate that passive porosity effectors can provide maneuver control increments that equal and exceed those of conventional aerodynamic effectors for low-speed, high-alpha flight, with control levels that are a linear function of porous area. This work demonstrates the tremendous potential of passive porosity to yield simple control effector systems that have no external moving parts and will preserve an aircraft's fixed outer mold line.

Hunter, Craig A.↗