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At least 1,567 records · Page 87
All sourcesNASA NTRSDOE OSTI
NASA NTRS1971

Laser modulation of optical absorption in ZnSe

The possibility of producing and detecting laser induced modulation of absorption (LIMA) in ZnSe crystals using a moderate laser intensity of about 6 mw output was investigated. The modulation is smaller than that previously obtained with higher laser photon flux, but is not too difficult to detect. Modulation of the order of a few parts in 10 to the 5th power, up to one part in 10 to the 4th power appears typical at the present. LIMA pulse shapes were recorded with varying system resolutions at a half dozen wavelengths in the visible, from 4800 A to 6000 A. Detailed data were taken, particularly at 5000 A, for a range of time intervals following laser pulse turn-on. Quantitative study of the excitation and decay kinetics of these is underway, though analysis is not complete. Major features are discussed with attention centered upon the detailed measurements made most recently at 5000 A.

Major, R. W.↗
NASA NTRS1987

A microstructural lattice model for strain oriented problems: A combined Monte Carlo finite element technique

A specialized, microstructural lattice model, termed MCFET for combined Monte Carlo Finite Element Technique, was developed which simulates microstructural evolution in material systems where modulated phases occur and the directionality of the modulation is influenced by internal and external stresses. In this approach, the microstructure is discretized onto a fine lattice. Each element in the lattice is labelled in accordance with its microstructural identity. Diffusion of material at elevated temperatures is simulated by allowing exchanges of neighboring elements if the exchange lowers the total energy of the system. A Monte Carlo approach is used to select the exchange site while the change in energy associated with stress fields is computed using a finite element technique. The MCFET analysis was validated by comparing this approach with a closed form, analytical method for stress assisted, shape changes of a single particle in an infinite matrix. Sample MCFET analytical for multiparticle problems were also run and in general the resulting microstructural changes associated with the application of an external stress are similar to that observed in Ni-Al-Cr alloys at elevated temperature.

Gayda, J.↗
NASA NTRS2008

John F. Kennedy Space Center's Technology Development and Application 2006-2007 Report

Topics covered include: Reversible Chemochromic Hydrogen Detectors; Determining Trajectory of Triboelectrically Charged Particles, Using Discrete Element Modeling; Using Indium Tin Oxide To Mitigate Dust on Viewing Ports; High-Performance Polyimide Powder Coatings; Controlled-Release Microcapsules for Smart Coatings for Corrosion Applications; Aerocoat 7 Replacement Coatings; Photocatalytic Coatings for Exploration and Spaceport Design; New Materials for the Repair of Polyimide Electrical Wire Insulation; Commodity-Free Calibration; Novel Ice Mitigation Methods; Crack Offset Measurement With the Projected Laser Target Device; New Materials for Structural Composites and Protective Coatings; Fire Chemistry Testing of Spray-On Foam Insulation (SOFI); Using Aerogel-Based Insulation Material To Prevent Foam Loss on the Liquid-Hydrogen Intertank; Particle Ejection and Levitation Technology (PELT); Electrostatic Characterization of Lunar Dust; Numerical Analysis of Rocket Exhaust Cratering; RESOLVE Projects: Lunar Water Resource Demonstration and Regolith Volatile Characterization; Tribocharging Lunar Soil for Electrostatic Beneficiation; Numerically Modeling the Erosion of Lunar Soil by Rocket Exhaust Plumes; Trajectory Model of Lunar Dust Particles; Using Lunar Module Shadows To Scale the Effects of Rocket Exhaust Plumes; Predicting the Acoustic Environment Induced by the Launch of the Ares I Vehicle; Measuring Ultrasonic Acoustic Velocity in a Thin Sheet of Graphite Epoxy Composite; Hail Size Distribution Mapping; Launch Pad 39 Hail Monitor Array System; Autonomous Flight Safety System - Phase III; The Photogrammetry Cube; Bird Vision System; Automating Range Surveillance Through Radio Interferometry and Field Strength Mapping Techniques; Next-Generation Telemetry Workstation; GPS Metric Tracking Unit; and Space-Based Range.

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NASA NTRS2011

NASA Tech Briefs, October 2011

Topics covered include: Laser Truss Sensor for Segmented Telescope Phasing; Qualifications of Bonding Process of Temperature Sensors to Deep-Space Missions; Optical Sensors for Monitoring Gamma and Neutron Radiation; Compliant Tactile Sensors; Cytometer on a Chip; Measuring Input Thresholds on an Existing Board; Scanning and Defocusing Properties of Microstrip Reflectarray Antennas; Cable Tester Box; Programmable Oscillator; Fault-Tolerant, Radiation-Hard DSP; Sub-Shot Noise Power Source for Microelectronics; Asynchronous Message Service Reference Implementation; Zero-Copy Objects System; Delay and Disruption Tolerant Networking MACHETE Model; Contact Graph Routing; Parallel Eclipse Project Checkout; Technique for Configuring an Actively Cooled Thermal Shield in a Flight System; Use of Additives to Improve Performance of Methyl Butyrate-Based Lithium-Ion Electrolytes; Li-Ion Cells Employing Electrolytes with Methyl Propionate and Ethyl Butyrate Co-Solvents; Improved Devices for Collecting Sweat for Chemical Analysis; Tissue Photolithography; Method for Impeding Degradation of Porous Silicon Structures; External Cooling Coupled to Reduced Extremity Pressure Device; A Zero-Gravity Cup for Drinking Beverages in Microgravity; Co-Flow Hollow Cathode Technology; Programmable Aperture with MEMS Microshutter Arrays; Polished Panel Optical Receiver for Simultaneous RF/Optical Telemetry with Large DSN Antennas; Adaptive System Modeling for Spacecraft Simulation; Lidar-Based Navigation Algorithm for Safe Lunar Landing; Tracking Object Existence From an Autonomous Patrol Vehicle; Rad-Hard, Miniaturized, Scalable, High-Voltage Switching Module for Power Applications; and Architecture for a 1-GHz Digital RADAR.

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NASA NTRS2013

Damping Effects of Drogue Parachutes on Orion Crew Module Dynamics

Currently, simulation predictions of the Orion Crew Module (CM) dynamics with drogue parachutes deployed are under-predicting the amount of damping as seen in free-flight tests. The Apollo Legacy Chute Damping model has been resurrected and applied to the Orion system. The legacy model has been applied to predict CM damping under drogue parachutes for both Vertical Spin Tunnel free flights and the Pad Abort-1 flight test. Comparisons between the legacy Apollo prediction method and test data are favorable. A key hypothesis in the Apollo legacy drogue damping analysis is that the drogue parachutes' net load vector aligns with the CM drogue attachment point velocity vector. This assumption seems reasonable and produces good results, but has never been quantitatively proven. The wake of the CM influences the drogue parachutes, which makes performance predictions of the parachutes difficult. Many of these effects are not currently modeled in the simulations. A forced oscillation test of the CM with parachutes was conducted in the NASA LaRC 20-Ft Vertical Spin Tunnel (VST) to gather additional data to validate and refine the Apollo legacy drogue model. A second loads balance was added to the original Orion VST model to measure the drogue parachute loads independently of the CM. The objective of the test was to identify the contribution of the drogues to CM damping and provide additional information to quantify wake effects and the interactions between the CM and parachutes. The drogue parachute force vector was shown to be highly dependent on the CM wake characteristics. Based on these wind tunnel test data, the Apollo Legacy Chute Damping model was determined to be a sufficient approximation of the parachute dynamics in relationship to the CM dynamics for preliminary entry vehicle system design. More wake effects should be included to better model the system. These results are being used to improve simulation model fidelity of CM flight with drogues deployed, which has been identified by the project as key to a successful Orion Critical Design Review.

Aubuchon, Vanessa V.↗
NASA NTRSDate not supplied

Py MILab: Capturing, Analyzing and Storing Test Data

Integrated Computational Materials Engineering (ICME) has recently received widespread attention due to its promises in reducing dependence on physical testing for engineering design by relying on simulation, reducing both time and cost to market for various applications. ICME however requires validated multiscale material models, which is heavily dependent on available test data with full material and test pedigree, including material processing, test and measurement equipment, raw data collection, and analysis methodology and results. Populating searchable information management systems with such rich data sets is often burdensome for data producers, resulting in a lack of findable data for modelers to validate and verify their models. To overcome these cultural barriers to ICME, NASA has developed of various database-integration toolsets that perform both data management activities within the organization’s best practices with additional functionality that relieves the effort of the data producer and promotes adoption of information management system. One such tool currently under development is Py MILab, an automatic framework for automatic capturing, analysis, maintenance, and storage of material test data. Py MILab uses a modular approach for capturing raw data, analyzing the data, and storing the data in a database, interfaced by neutral file structures, to promote plug-and-play capabilities for various analysis types. TMAnalysis is a Python-based tool that performs automatic data reduction and analysis of uniaxial thermomechanical test data. The TMAnalysis toolset can be implemented within the Analysis module of Py MILab, and thus requires a populated neutral file form the Raw Data Module of Py MILab and outputs a Analysis neutral file compatible with the Database Module of Py MILab. TMAnalysis is able to perform automatic segmentation of multistage tests and perform data analysis and reduction, including determination of point-wise properties in tension, compression, and shear, analysis of stress relaxation tests, creep analysis and zone identification, and combination of these stage types for tests with complex loading histories. The TMAnalysis code is accompanied with a graphical user interface (GUI) that allows users to easily analyze test data in bulk, verify the automatic, consistent analysis performed by the backend code, and edit stage segmentation if necessary before producing the output neutral files, ensuring data is properly analyzed and maintained with full traceability.

Data management↗
DOE OSTI2024

OpenFAST Modeling of the T-Omega Wind Floating Offshore Wind Turbine System: Preprint

To reduce the cost of offshore wind energy through a more efficient design of the floating support structure, T-Omega Wind developed a novel lightweight and shallow-draft platform concept that aims to achieve a wave-following behavior without resonance amplification. The rotor and generator are carried by four tower legs with each leg supported by a shallow-draft float at the base. In preparation for a more detailed analysis, a coupled aero-hydro-elastic model of the proposed concept is developed in OpenFAST. The detailed modeling approach is presented, including a novel application of the SubDyn substructure dynamics module of OpenFAST to approximate the loads on the axle tube at the tower top from the rotor hub bearings. Preliminary results obtained with the OpenFAST model by rigidifying the substructure and blades indicate that the original sizing of the design can lead to large hub acceleration in the axial/surge direction and in the pitch direction due to platform-pitch motion. The high hub acceleration also potentially leads to large bending moments in the four tower legs. To address the issue identified, an updated design is developed with, among other changes, increased float spacing at the tower base and improved geometry of the tower legs. The new design suggests significantly reduced extreme platform pitch angles under the same conditions and will be further analyzed in the future through full aero-hydro-servo-elastic simulations.

FOWT↗
DOE OSTI2020

N -Arylimide Molecular Balances: A Comprehensive Platform for Studying Aromatic Interactions in Solution

Noncovalent interactions of aromatic surfaces play a key role in many biological processes and in determining the properties and utility of synthetic materials, sensors, and catalysts. However, the study of aromatic interactions has been challenging because these interactions are usually very weak and their trends are modulated by many factors such as structural, electronic, steric, and solvent effects. Recently, N-arylimide molecular balances have emerged as highly versatile and effective platforms for studying aromatic interactions in solution. These molecular balances can accurately measure weak noncovalent interactions in solution via their influence on the folded–unfolded conformational equilibrium. The structure (i.e., size, shape, π-conjugation, and substitution) and nature (i.e., element, charge, and polarity) of the π-surfaces and interacting groups can be readily varied, enabling the study of a wide range of aromatic interactions. These include aromatic stacking, heterocyclic aromatic stacking, and alkyl–π, chalcogen–π, silver–π, halogen–π, substituent–π, and solvent–π interactions. The ability to measure a diverse array of aromatic interactions within a single model system provides a unique perspective and insights as the interaction energies, stability trends, and solvent effects for different types of interactions can be directly compared. Some broad conclusions that have emerged from this comprehensive analysis include: (1) The strongest aromatic interactions involve groups with positive charges such as pyridinium and metal ions which interact with the electrostatically negative π-face of the aromatic surface via cation–π or metal–π interactions. Attractive electrostatic interactions can also form between aromatic surfaces and groups with partial positive charges. (2) Electrostatic interactions involving aromatic surfaces can be switched from repulsive to attractive using electron-withdrawing substituents or heterocycles. These electrostatic trends appear to span many types of aromatic interactions involving a polar group interacting with a π-surface such as halogen–π, chalcogen–π, and carbonyl–π. (3) Nonpolar groups form weak but measurable stabilizing interactions with aromatic surfaces in organic solvents due to favorable dispersion and/or solvophobic effects. Furthermore, a good predictor of the interaction strength is provided by the change in solvent-accessible surface area. (4) Solvent effects modulate the aromatic interactions in the forms of solvophobic effects and competitive solvation, which can be modeled using solvent cohesion density and specific solvent–solute interactions.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
NASA NTRS1979

ATLAS, an integrated structural analysis and design system. Volume 3: User's manual, input and execution data

The input data and execution control statements for the ATLAS integrated structural analysis and design system are described. It is operational on the Control Data Corporation (CDC) 6600/CYBER computers in a batch mode or in a time-shared mode via interactive graphic or text terminals. ATLAS is a modular system of computer codes with common executive and data base management components. The system provides an extensive set of general-purpose technical programs with analytical capabilities including stiffness, stress, loads, mass, substructuring, strength design, unsteady aerodynamics, vibration, and flutter analyses. The sequence and mode of execution of selected program modules are controlled via a common user-oriented language.

Dreisbach, R. L.↗
NASA NTRS1986

Independent Orbiter Assessment (IOA): Analysis of the rudder/speed brake subsystem

The results of the Independent Orbiter Assessment (IOA) of the Failure Modes and Effects Analysis (FMEA) and Critical Items List (CIL) are presented. The IOA approach features a top-down analysis of the hardware to determine failure modes, criticality, and potential critical items. To preserve independence, this analysis was accomplished without reliance upon the results contained within the NASA FMEA/CIL documentation. The independent analysis results for the Orbiter Rudder/Speedbrake Actuation Mechanism is documented. The function of the Rudder/Speedbrake (RSB) is to provide directional control and to provide a means of energy control during entry. The system consists of two panels on a vertical hinge mounted on the aft part of the vertical stabilizer. These two panels move together to form a rudder but split apart to make a speedbrake. The Rudder/Speedbrake Actuation Mechanism consists of the following elements: (1) Power Drive Unit (PDU) which is composed of hydraulic valve module and a hydraulic motor-powered gearbox which contains differentials and mixer gears to provide PDU torque output; (2) four geared rotary actuators which apply the PDU generated torque to the rudder/speedbrake panels; and (3) ten torque shafts which join the PDU to the rotary actuators and interconnect the four rotary actuators. Each level of hardware was evaluated and analyzed for possible failures and causes. Criticality was assigned based upon the severity of the effect for each failure mode. Critical RSB failures which result in potential loss of vehicle control were mainly due to loss of hydraulic fluid, fluid contaminators, and mechanical failures in gears and shafts.

Wilson, R. E.↗
NASA NTRS1994

A Planar Approximation for the Least Reliable Bit Log-likelihood Ratio of 8-PSK Modulation

The optimum decoding of component codes in block coded modulation (BCM) schemes requires the use of the log-likelihood ratio (LLR) as the signal metric. An approximation to the LLR for the least reliable bit (LRB) in an 8-PSK modulation based on planar equations with fixed point arithmetic is developed that is both accurate and easily realizable for practical BCM schemes. Through an error power analysis and an example simulation it is shown that the approximation results in 0.06 dB in degradation over the exact expression at an E(sub s)/N(sub o) of 10 dB. It is also shown that the approximation can be realized in combinatorial logic using roughly 7300 transistors. This compares favorably to a look up table approach in typical systems.

Thesling, William H.↗
NASA NTRS2002

Submillimeter Laboratory Investigations: Spectroscopy and Collisions

Currently, millimeter-wave and submillimeter-wave spectroscopy is conducted in our laboratory on several different types of spectrometers. Our standard spectrometer utilizes the output of a phase-locked klystron operating in the 40-60 GHz region, which is sent into a crossed-waveguide harmonic generator, or "multiplier". The high frequency millimeter-and submillimeter-wave radiation is transmitted via quasi-optical techniques through an absorption cell and then onto a detector, which is either an InSb hot electron bolometer cooled to 1.4 K or a Si bolometer cooled to 0.3 K. The detector response is sent to a computer for measurement and analysis. The frequency range produced and detected in this manner goes from 80 GHz to upwards of 1 THz. Spectra are normally taken with source modulation, with line frequencies typically measured to an accuracy of 50-100 kHz. Higher accuracy is available when needed. Recently, we developed a new, broad-band spectrometer in our laboratory based on a free-running backward wave oscillator (BWO) of Russian manufacture as the primary source of radiation. The so-called FASSST (fast-scan submillimeter spectroscopic technique) system uses fast-scan and optical calibration methods rather than the traditional locking techniques. The output power from the BWO is split such that 90% goes into the absorption cell while 10% is coupled to a 40-meter Fabry-Perot cavity, which yields fringe? for frequency measurement. Results from this spectrometer on the spectrum of nitric acid (HNO3) show that 100 GHz of spectral data can be obtained in 5 seconds with a measurement accuracy of 50 kHz. Currently, the frequency range of the FASSST system in our laboratory is roughly 100-700 GHz.

Herbst, Eric↗
NASA NTRS2011

International Space Station Acoustics - A Status Report

It is important to control acoustic noise aboard the International Space Station (ISS) to provide a satisfactory environment for voice communications, crew productivity, and restful sleep, and to minimize the risk for temporary and permanent hearing loss. Acoustic monitoring is an important part of the noise control process on ISS, providing critical data for trend analysis, noise exposure analysis, validation of acoustic analysis and predictions, and to provide strong evidence for ensuring crew health and safety, thus allowing Flight Certification. To this purpose, sound level meter (SLM) measurements and acoustic noise dosimetry are routinely performed. And since the primary noise sources on ISS include the environmental control and life support system (fans and airflow) and active thermal control system (pumps and water flow), acoustic monitoring will indicate changes in hardware noise emissions that may indicate system degradation or performance issues. This paper provides the current acoustic levels in the ISS modules and sleep stations, and is an update to the status presented in 20031. Many new modules, and sleep stations have been added to the ISS since that time. In addition, noise mitigation efforts have reduced noise levels in some areas. As a result, the acoustic levels on the ISS have improved.

Allen, Christopher S.↗
NASA NTRS2019

James Webb Space Telescope Optical Telescope Element / Integrated Science Instrument Module (OTIS) Cryogenic Vacuum Test Part I: Thermal Architecture

The James Webb Space Telescope (JWST), set to launch in early 2019, is currently undergoing a series of system-level environmental tests to verify its workmanship and end-to-end functionality. As part of this series, the Optical Telescope Element and Integrated Science Instrument Module (OTIS) Cryo-Vacuum (CV) test, the most complex cryogenic test executed to date by NASA, was completed in 2017 at the Johnson Space Center's Chamber A facility. The OTIS CV test was intended as a comprehensive test of the integrated instrument and telescope systems to fully understand its optical, structural, and thermal performance within its intended flight environment. Due to its complexity, extensive pre-test planning was required to ensure payload safety and compliance with all limits and constraints. A comprehensive thermal analysis campaign was undertaken to predict the behavior of the test payload during its transition to cryogenic temperatures and back to ambient, and exhaustive preparations for off-nominal scenarios were studied due to the increased possibility of unanticipated events during the 100-day test length. This current four-part online course for the NASA Engineering and Safety Center (NESC) Academy gives a thorough overview of the mutiple years of preparation executed by hundreds of individuals to ensure the success of the JWST OTIS CV Test. Part I will introduce the major components of James Webb and specifically the OTIS CV Test thermal architecture. Part II will encompass the extensive thermal analysis performed to prepare for the test. Part III will talk about the preparations for off-nominal events: what analysis was done, and how steps were taken pre-test to anticipate unexpected circumstances and mitigate their impacts to hardware and test timeline. Part IV is a recap of the lessons learned from the thermal perspective for both the payload and ground support equipment (GSE) test conductors. It is hoped that the insight gained from the OTIS CV test campaign will benefit planning and execution for upcoming large cryogenic missions.

OTIS↗
NASA NTRSDate not supplied

The Representation of Aerosols in GMAO’s Newest Reanalyses

Over the past few years, NASA’s Global Modelling and Assimilation Office has been working on the configuration and production of three new reanalysis products, GEOS-IT, GiOcean, and MERRA-21C. GEOS-IT, or the Goddard Earth Observing System for Instrument Teams, is a 3D variational data assimilation system that runs in a near real time framework however retrospectively provides data back through 1998 to deliver a consistent view of the Earth-atmosphere system for the production of observational NASA products. Retrospective production for GEOS-IT is complete and the meteorology has since been used to produce the one way weakly coupled GiOcean reanalysis. Due to differences in the atmospheric model, particularly related to scavenging, aerosols are not identical in GEOS-IT and GiOcean. MERRA-21C, or the Modern Era Restrospective analysis for Research and Applications in the 21st century, is a hybrid 4D ensemble variational system at a finer horizontal resolution of 0.25 degrees. Although different in their intended use, and therefore configuration, these systems prominently feature coupling between meteorology and aerosols. The differences and similarities in the aerosol configuration between the three systems will be discussed, covering biomass burning and anthropogenic emissions as well as observations used for the assimilation of aerosol optical depth. A large emphasis will be placed on the version of the underlying aerosol module, GOCART, which underwent a complete refactoring and the addition of radiatively active brown carbon between GEOS-IT and MERRA-21C. Independent observations will be used to evaluate the performance of aerosols in both reanalyses, focusing on aerosol optical depth, surface particulate matter, and vertical profiles of aerosol backscatter.

Allison Collow↗
DOE OSTI2023

Next Generation Durable, Cost Effective, Energy Efficient Tubular SOFC (Final Scientific/Technical Report)

The overall objective of this project is to develop and optimize a YSZ electrolyte-based solid oxide fuel cell (SOFC) technology for low cost, low temperature (~ 650°C), and high energy efficiency operation. The developed technology will be implemented and demonstrated in a high efficiency 2~3kW SOFC with applicability to sub-MW systems. A summary of significant accomplishments is provided below. Key accomplishments: 1. Improved fundamental cell technology demonstrated at single tube and system scale. Improved power output by 54% while operating at the normal temperature of 750°C. And improved power output by 33% while operating at 650°C, 100°C lower than normal temperature. 2. SPS patented internal recycle arrangement was developed and extended to operate on LPG fuel directly in a compact, high-efficiency (> 40%) system. 3. System testing was conducted to prove the long-term durability of cell improvements. Demonstrated over 8000 hours of operation at 0.21 %/1000 hrs degradation. 4. A large-scale, 2.5kW net power system demonstration was completed, which demonstrated 40% net efficiency over 1000 hours. 5. System design and cost analysis of a 1MW system utilizing a 2.5kW bundle was completed. Cost optimization of the bundle showed a reduction of nearly 80% is possible from $\$$5,790/kW to $\$$1250/kW. This lower cost is considered viable for SPS commercialization.

03 NATURAL GAS↗
DOE OSTI2023

Life-Cycle Analysis of Potentially Longer Life Expectancy CdTe PV Modules

Research on PV module longevity shows that with lower degradation rates and better encapsulation, PV modules can significantly outlast their current life expectancy of 25-30 years. The focus of this study is on alternative CdTe PV module back-contact and encapsulation materials that show the potential of increasing module operation lives from 30 to 40 and 50 years. Life-cycle-analyis shows that some alternative materials may slightly increase cradle-to-gate environmental impacts per m2 of module, but increased operational life more than counterbalances such impacts. The life-cycle carbn footprint of systems operating under average US insolation conditions of 1800 kWh/m2/yr is reduced from 10 gCO2eq/kWh to 6 gCO2eq/kWh when the operation life increases from 30 to 50 yrs. Similar reductions are noted for all life-cycle impact indicators, whereas the EROI of the considered system increases from 67 to 177 as life increases from 30 to 50 yrs.

14 SOLAR ENERGY↗
DOE OSTI2022

Machine-learning from Pseudomonas putida KT2440 transcriptomes reveals its transcriptional regulatory network

Bacterial gene expression is orchestrated by numerous transcription factors (TFs). Elucidating how gene expression is regulated is fundamental to understanding bacterial physiology and engineering it for practical use. In this study, a machine-learning approach was applied to uncover the genome-scale transcriptional regulatory network (TRN) in Pseudomonas putida KT2440, an important organism for bioproduction. We performed independent component analysis of a compendium of 321 high-quality gene expression profiles, which were previously published or newly generated in this study. We identified 84 groups of independently modulated genes (iModulons) that explain 75.7% of the total variance in the compendium. With these iModulons, we (i) expand our understanding of the regulatory functions of 39 iModulon associated TFs (e.g., HexR, Zur) by systematic comparison with 1993 previously reported TF-gene interactions; (ii) outline transcriptional changes after the transition from the exponential growth to stationary phases; (iii) capture group of genes required for utilizing diverse carbon sources and increased stationary response with slower growth rates; (iv) unveil multiple evolutionary strategies of transcriptome reallocation to achieve fast growth rates; and (v) define an osmotic stimulon, which includes the Type VI secretion system, as coordination of multiple iModulon activity changes. Taken together, this study provides the first quantitative genome-scale TRN for P. putida KT2440 and a basis for a comprehensive understanding of its complex transcriptome changes in a variety of physiological states.

09 BIOMASS FUELS↗