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At least 1,567 records · Page 87

All sources NASA NTRS DOE OSTI

DOE OSTI2012

Complete Genome Sequence of Desulfurococcus fermentans, a Hyperthermophilic Cellulolytic Crenarchaeon Isolated from a Freshwater Hot Spring in Kamchatka, Russia

Desulfurococcus fermentans is the first known cellulolytic archaeon. This hyperthermophilic and strictly anaerobic crenarchaeon produces hydrogen from fermentation of various carbohydrates and peptides without inhibition by accumulating hydrogen. The complete genome sequence reported here suggested that D. fermentans employs membrane-bound hydrogenases and novel glycohydrolases for hydrogen production from cellulose.

ARCHAEON↗

DOE OSTI2012

Complete Genome Sequence of the Thermophilic, Piezophilic, Heterotrophic Bacterium Marinitoga piezophila KA3

Marinitoga piezophila KA3 is a thermophilic, anaerobic, chemoorganotrophic, sulfur-reducing bacterium isolated from the Grandbonum deep-sea hydrothermal vent site at the East Pacific Rise (13 degrees N, 2,630-m depth). The genome of M. piezophila KA3 comprises a 2,231,407-bp circular chromosome and a 13,386-bp circular plasmid. This genome was sequenced within Department of Energy Joint Genome Institute CSP 2010.

ORDER THERMOTOGALES↗

DOE OSTI2012

Whole-Genome Sequences of Borrelia bissettii Borrelia valaisiana and Borrelia spielmanii

It has been known for decades that human Lyme disease is caused by the three spirochete species Borrelia burgdorferi, Borrelia afzelii, and Borrelia garinii. Recently, Borrelia valaisiana, Borrelia spielmanii, and Borrelia bissettii have been associated with Lyme disease. We report the complete genome sequences of B. valaisiana VS116, B. spielmanii A14S, and B. bissettii DN127.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2021

Complete Genome Sequence of Desulfobulbus oligotrophicus Prop6, an Anaerobic Deltabacterota Strain That Lacks Mercury Methylation Capability

Desulfobulbus oligotrophicus Prop6 is a sulfate-reducing, propionate-oxidizing Deltabacterota (formerly Deltaproteobacteria) strain from sewage sludge. Desulfobulbus species are found in anoxic environments, in animal microbiota, and some produce the neurotoxin methylmercury. The 3.1-Mbp D. oligotrophicus genome sequence enables studies of diverse environmental adaptations and the evolutionary genomics of mercury methylation mechanisms.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2023

Draft Genome Sequence of Pedococcus sp. Strain 5OH_020, Isolated from California Grassland Soil

The draft genome sequence of the soil bacterium Pedococcus sp. strain 5OH_020, isolated on a natural cobalamin analog, comprises 4.4 Mbp, with 4,108 protein-coding genes. Its genome encodes cobalamin-dependent enzymes, including methionine synthase and class II ribonucleotide reductase. Taxonomic analysis suggests that it is a novel species within the genus Pedococcus .

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2021

Complete Genome Sequence of Human Oral Actinomyces sp. HMT897 Strain ORNL0104, a Host of the Saccharibacterium (TM7) HMT351

Actinomyces sp. HMT897 strain ORNL0104 was isolated from a human saliva sample and can serve as a host for the ectobiont Saccharibacteria (TM7) HMT351. Its 3.3-Mb chromosome was completely sequenced using PacBio long reads, and it encodes 2,407 proteins and 63 RNAs.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2022

Near-Complete Genome Sequence of Zygosaccharomyces rouxii NRRL Y-64007, a Yeast Capable of Growing on Lignocellulosic Hydrolysates

The halotolerant and osmotolerant yeast Zygosaccharomyces rouxii can produce multiple volatile compounds and has the ability to grow on lignocellulosic hydrolysates. We report the annotated genome sequence of Z. rouxii NRRL Y-64007 to support its development as a platform organism for biofuel and bioproduct production.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2019

Improved Draft Genome Sequence of Bacillus sp. Strain YF23, Which Has Plant Growth-Promoting Activity

We report here the improved draft genome sequence of Bacillus sp. strain YF23, a bacterium originally isolated from switchgrass ( Panicum virgatum ) plants and shown to exhibit plant growth-promoting activity. The genome comprised 5.82 Mbp, containing 5,933 genes, with 193 as RNA genes, and a GC content of 35.10%.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2020

Draft Genome Sequence of Yokenella regensburgei Strain WCD67, Isolated from the Boxelder Bug

We report here the draft genome sequence of Yokenella regensburgei strain WCD67, isolated from the boxelder bug ( Boisea trivittata ). The genome is 5,277,883 bp in size, has a GC content of 54.12%, and has 5,416 genes. A total of 17 mobile elements were discovered, 6 of which were predicted to be phages.

Meyers, Desiree J.↗

DOE OSTI2024

Metagenomic sequencing of a Patescibacteria-containing enrichment from Zodletone spring in Oklahoma, USA

An enrichment of sulfidic sediments from Zodletone spring was sequenced as a metagenome. Draft genomes representing Cloacimonadota, Deltabacterota, Firmicutes, and Patescibacteria were binned and annotated and will aid functional genomics and cultivation efforts.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2019

Gene Determinants for Mercury Bioremediation as Revealed by Draft Genome Sequence Analysis of Stenotrophomonas sp. Strain MA5

A soilborne Stenotrophomonas sp. strain (MA5) that is resistant to mercury was isolated. A draft genome sequence-based analysis revealed a suite of gene determinants to resist mercury and other heavy metals, multidrug efflux, stress response, and membrane transport, and these provide cues to a suite of mechanisms that underpin cellular survival in contaminated soil.

Pathak, Ashish↗

DOE OSTI2022

Complete Genome Sequence of Bacillus cereus Strain CPT56D-587-MTF, Isolated from a Nitrate- and Metal-Contaminated Subsurface Environment

Bacillus cereus strain CPT56D-587-MTF was isolated from nitrate- and toxic metal-contaminated subsurface sediment at the Oak Ridge Reservation (ORR) (Oak Ridge, TN, USA). Here, we report the complete genome sequence of this strain to provide genomic insight into its strategies for survival at this mixed-waste site.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2023

Complete Genome Sequence of Desulfomicrobium sp. Strain ZS1 from Zodletone Spring in Oklahoma, USA

Desulfomicrobium sp. strain ZS1 is an obligate anaerobic, sulfate-reducing member of the Desulfobacterota from Zodletone Spring, an anoxic sulfide-rich spring in southwestern Oklahoma. Its complete genome was sequenced using a combination of Illumina and Oxford Nanopore platforms and encodes 3,364 proteins and 81 RNAs on a single chromosome.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2021

Metagenome-Assembled Genome Sequences of Novel Prokaryotic Species from the Mercury-Contaminated East Fork Poplar Creek, Oak Ridge, Tennessee, USA

We sequenced two metagenomes of sediments from the East Fork Poplar Creek in the Oak Ridge Reservation (Oak Ridge, TN), a natural stream that has been contaminated with Hg from upstream sources, and we reconstructed 28 metagenome-assembled genomes of novel prokaryotic species.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2020

Draft Genome Sequence of Larkinella sp. Strain BK230, Isolated from Populus deltoides Roots

Larkinella sp. strain BK230, a heterotrophic bacterium of the phylum Bacteroidetes , was isolated from the roots of a field-grown eastern cottonwood tree ( Populus deltoides ) located in Georgia. The draft 7.27-Mb genome has a G+C content of 53.4% and contains 6,026 coding sequences, including 41 tRNA genes.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2021

Complete Genome Sequences of Two Gammaproteobacterial Methanotrophs Isolated from a Mercury-Contaminated Stream

The genomes of Methylomonas sp. strain EFPC1 and Methylococcus sp. strain EFPC2 isolated from a mercury-contaminated stream in Oak Ridge Tennessee, were sequenced.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2022

Draft Genome Sequences of 10 Pseudomonas sp. Isolates from the Active Layer of Permafrost in Ny Ålesund, Svalbard, Norway

Ten distinct isolates from the genus Pseudomonas were isolated in culture. The genomes of these isolates were sequenced using the Illumina MiSeq platform and assembled in order to provide insight into the metabolic and carbon-degrading potential of bacteria residing in soils at high latitudes.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2025

Draft genome sequence of Rossellomorea marisflavi DL-A, a malathion-degrading bacterium

This study presents the draft genome of Rossellomorea marisflavi DL-A, a malathion-degrading bacterium isolated from the North Oconee River in Athens, GA, USA. The DL-A genome is 4.27 Mb in size and comprises 4,271 coding sequences with proteins.

59 BASIC BIOLOGICAL SCIENCES↗