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DOE OSTI2023

Final Scientific/Technical Report: Real Time Particle Size and Settling Velocity for In-Situ Monitoring of Deep-Sea Polymetallic Nodule Mining

In the coming decade, it is anticipated that deep-sea mining activities will commence throughout the deep ocean, at depths around 4000-6000m where vast deposits of baseball-sized rocks called polymetallic nodules lay on the seabed. These nodules contain resources such as cobalt, copper and nickel which are needed to produce batteries for electric vehicles. Collector machines driving on the seabed and gathering these nodules will stir up clouds of sediment, and there is concern about how the resulting concentration of sediment in the near-bottom ocean water will affect deep and mid-ocean biology. There is currently no technology capable of comprehensively monitoring the concentrations and properties of stirred up sediment in the deep. This project addressed this critical gap by developing a deep-sea particle measurement system (Real-Time Size and Settling Velocity, RTSSV) that uses a multi-camera high-resolution video imaging system, with accompanying image processing, to measure the concentration, size distribution and settling speed of sediment. Importantly, these measurements are made in-situ, so that delicate sediment aggregates will not be disturbed, as is the case when collecting samples at depth to be measured in the lab. The instrument can be mounted on a variety of established deep-sea platforms such as moorings, AUVs and ROVs, providing data in near real time, where the key measurements need to be made—from the source of the sediment disturbance and throughout the resulting plume—vital for determining if deep-sea mining can be done in an environmentally responsible manner. The developed system (RTSSV) will possess the only such capability worldwide, giving a US-based group a substantial competitive advantage as this newly evolving global industry develops. As added value, the technology will also have widespread application to the offshore oil and gas industry, dredging industry, and to sediment science research.

47 OTHER INSTRUMENTATION↗

DOE OSTI2020

Fungal Community, Not Substrate Quality, Drives Soil Microbial Function in Northeastern U.S. Temperate Forests

Mycorrhizal fungi can affect soil organic matter cycling through several mechanisms including priming, nutrient competition, and direct enzyme production. Differences in nutrient foraging strategies between ectomycorrhizal (EcM) and arbuscular mycorrhizal (AM) fungi produce divergent belowground dynamics: where EcM can take up organic nitrogen and directly break down soil organic matter (SOM) by producing enzymes, AM fungi are limited to scavenging mineral N. EcM-associated tree species also have leaf litter with relatively higher ratios of carbon to nitrogen (C:N), and belowground saprotrophic communities more dominated by fungi. Consequently, free-living microbes in EcM-dominated soils should experience nitrogen limitation, with subsequent increases in enzyme production and decreased carbon use efficiency (CUE). However, the relative importance of the effects of substrate quality and fungal community composition on enzyme production and CUE are unclear. To assess this distinction, we sampled the organic horizon and 10 cm of the mineral horizon in northern temperate forest soils along a gradient of EcM dominance. We characterized fungal community composition by measuring EcM relative abundances from extracted fungal DNA and the fungal to bacterial (F:B) ratios from phospholipid fatty acid (PLFA) analysis. We assessed soil substrate quality as the soil C:N ratio. Soil microbial functions were measured as potential activities of five hydrolytic and two oxidative enzymes, and microbial CUE. We found that the fungal community, represented by either the F:B ratio, EcM relative abundance, or both, affected CUE and six measured enzyme activities, while the C:N ratio affected only oxidative and chitin-targeting extracellular enzyme activities. Our results highlight the use of EcM relative dominance as a predictor of soil microbial community composition and function independent of substrate quality.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2023

BONCAT-FACS-Seq reveals the active fraction of a biocrust community undergoing a wet-up event

Determining which microorganisms are active within soil communities remains a major technical endeavor in microbial ecology research. One promising method to accomplish this is coupling bioorthogonal non-canonical amino acid tagging (BONCAT) with fluorescence activated cell sorting (FACS) which sorts cells based on whether or not they are producing new proteins. Combined with shotgun metagenomic sequencing (Seq), we apply this method to profile the diversity and potential functional capabilities of both active and inactive microorganisms in a biocrust community after being resuscitated by a simulated rain event. We find that BONCAT-FACS-Seq is capable of discerning the pools of active and inactive microorganisms, especially within hours of applying the BONCAT probe. The active and inactive components of the biocrust community differed in species richness and composition at both 4 and 21 h after the wetting event. The active fraction of the biocrust community is marked by taxa commonly observed in other biocrust communities, many of which play important roles in species interactions and nutrient transformations. Among these, 11 families within the Firmicutes are enriched in the active fraction, supporting previous reports indicating that the Firmicutes are key early responders to biocrust wetting. We highlight the apparent inactivity of many Actinobacteria and Proteobacteria through 21 h after wetting, and note that members of the Chitinophagaceae, enriched in the active fraction, may play important ecological roles following wetting. Based on the enrichment of COGs in the active fraction, predation by phage and other bacterial members, as well as scavenging and recycling of labile nutrients, appear to be important ecological processes soon after wetting. To our knowledge, this is the first time BONCAT-FACS-Seq has been applied to biocrust samples, and therefore we discuss the potential advantages and shortcomings of coupling metagenomics to BONCAT to intact soil communities such as biocrust. In all, by pairing BONCAT-FACS and metagenomics, we are capable of highlighting the taxa and potential functions that typifies the microbes actively responding to a rain event.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1971

A summary of phase analysis on Apollo 14 samples

The results of an analysis of lunar samples from Apollo 14 are presented. The large number of breccias returned from the Fra Mauro formation show that impact events are an important rock forming mechanism on the moon. Larger rocks as well as micro breccias bear structural resemblance to brecciated chondrites and terrestrial impactites. Many show evidence of repetitious events of break-up and accumulation welding. The surface of the regolith has become thoroughly mixed by this process. Most components however appear locally derived from basalts rich in feldspar and Kreep components, similar to rocks such as 14310.

Fredriksson, K.↗

NASA NTRS1975

Attenuation of sound in ducts with acoustic treatment: A generalized approximate equation

A generalized approximate equation for duct lining sound attenuation is presented. The specification of two parameters, the maximum possible attenuation and the optimum wall acoustic impedance is shown to completely determine the sound attenuation for any acoustic mode at any selected wall impedance. The equation is based on the nearly circular shape of the constant attenuation contours in the wall acoustic impedance plane. For impedances far from the optimum, the equation reduces to Morse's approximate expression. The equation can be used for initial acoustic liner design. Not least important is the illustrative nature of the solutions which provide an understanding of the duct propagation problem usually obscured in the exact calculations. Sample calculations using the approximate attenuation equation show that the peak and the bandwidth of the sound attenuation spectrum can be represented by quite simple functions of the ratio of actual wall acoustic resistance to optimum resistance.

Rice, E. J.↗

NASA NTRS1975

Attenuation of sound in ducts with acoustic treatment - A generalized approximate equation

Rice, E. J.↗

NASA NTRS1979

Measurement of chlorophyll a fluorescence with an airborne fluorosensor

Phytoplankton biomass and diversity among various algal species are important for marine productivity assessments. The spatial heterogeneity of phytoplankton in coastal and estuarine environments complicates estimates of total biomass using conventional surface sampling techniques. Since synoptic or near-synoptic data can be quite useful in these studies, this area is a natural focal point for development of remote sensors. However, it is very difficult to sense phytoplankton density and diversity with spacecraft-borne passive sensors primarily because modulation in the signal due to phytoplankton is of the same order as that of atmospheric effects. The same sensors mounted on aircraft may be able to detect and quantify high concentrations of phytoplankton (blooms), but the current lack of knowledge about the spectral reflectance signatures of the major phytoplankton color groups rules out any diversity measurements by this type of sensor. An active fluorosensor mounted on a low-flying aircraft or helicopter is not limited by any of these constraints. A brief survey of the four currently active systems is presented.

Jarrett, O., Jr.↗

NASA NTRS1982

Intagliated phosphor screen image tube project

The production and evaluation of a magnetic focus image tube for astronomical photography that has an intagliated phosphor screen is described. The modulation transfer function of such a tube was measured by electronic means and by film tests, and the results compared with tubes of more conventional construction. The physical properties of the image tube and film combination, the analytical model of the optical interface, and the salient features of the intagliated screen tube are described. The results of electronic MTF tests of the intagliated image tube and of the densitometry of the tube and film test samples are presented. It is concluded that the intagliated screen is a help, but that the thickness of the photographic film is also important.

Hertzel, R. J.↗

NASA NTRS1982

Airborne aerosol measurements in the quiescent plume of Mount Saint Helens September, 1980

A study of the emissions from the Mt. St. Helens volcano was conducted to obtain data for an assessment of the importance of volcanoes as a global source of volatile trace elements to the atmosphere and to the global biochemical cycles of these elements. Sampling was done on board an Orion P-3 turboprop aircraft modified for tropospheric aerosol and gas sampling. Two filter collection systems were used. Samples were collected on a single flight on September 22, 1980, at which time the volcano was emitting a stable plume to an altitude of between 2 and 3 km. The results regarding the concentrations of aerosols obtained for this mission are presented in a table. Attention is also given to data concerning particulate vs. gas phase sulfur in the Mt. Saint Helens plume, and the estimated volcanic particle flux of selected volatile elements.

Phelan, J. M.↗

NASA NTRS1982

An improved algorithm for evaluating trellis phase codes

A method is described for evaluating the minimum distance parameters of trellis phase codes, including CPFSK, partial response FM, and more importantly, coded CPM (continuous phase modulation) schemes. The algorithm provides dramatically faster execution times and lesser memory requirements than previous algorithms. Results of sample calculations and timing comparisons are included.

Mulligan, M. G.↗

NASA NTRS1983

Rain estimation from satellites: An examination of the Griffith-Woodley technique

The Griffith-Woodley Technique (GWT) is an approach to estimating precipitation using infrared observations of clouds from geosynchronous satellites. It is examined in three ways: an analysis of the terms in the GWT equations; a case study of infrared imagery portraying convective development over Florida; and the comparison of a simplified equation set and resultant rain map to results using the GWT. The objective is to determine the dominant factors in the calculation of GWT rain estimates. Analysis of a single day's convection over Florida produced a number of significant insights into various terms in the GWT rainfall equations. Due to the definition of clouds by a threshold isotherm the majority of clouds on this day did not go through an idealized life cycle before losing their identity through merger, splitting, etc. As a result, 85% of the clouds had a defined life of 0.5 or 1 h. For these clouds the terms in the GWT which are dependent on cloud life history become essentially constant. The empirically derived ratio of radar echo area to cloud area is given a singular value (0.02) for 43% of the sample, while the rainrate term is 20.7 mmh-1 for 61% of the sample. For 55% of the sampled clouds the temperature weighting term is identically 1.0. Cloud area itself is highly correlated (r=0.88) with GWT computed rain volume. An important, discriminating parameter in the GWT is the temperature defining the coldest 10% cloud area. The analysis further shows that the two dominant parameters in rainfall estimation are the existence of cold cloud and the duration of cloud over a point.

Negri, A. J.↗

NASA NTRS1984

Rain estimation from satellites - An examination of the Griffith-Woodley Technique

The Griffith-Woodley Technique (GWT) is an approach to estimating precipitation using infrared observations of clouds from geosynchronous satellites. It is examined in three ways: an analysis of the terms in the GWT equations; a case study of infrared imagery portraying convective development over Florida; and the comparison of a simplified equation set and resultant rain map to results using the GWT. The objective is to determine the dominant factors in the calculation of GWT rain estimates. Analysis of a single day's convection over Florida produced a number of significant insights into various terms in the GWT rainfall equations. Due to the definition of clouds by a threshold isotherm the majority of clouds on this day did not go through an idealized life cycle before losing their identity through merger, splitting, etc. As a result, 85 percent of the clouds had a defined life of 0.5 or 1 h. For these clouds, the terms in the GWT which are dependent on cloud life history become essentially constant. The empirically derived ratio of radar echo area to cloud area is given a singular value (0.02) for 43 percent of the sample, while the rainrate term is 20.7 mmh-1 for 61 percent of the sample. For 55 percent of the sampled clouds, the temperature weighting term is identically 1.0. Cloud area itself is highly correlated (r = 0.88) with GWT computed rain volume. An important, discriminating parameter in the GWT is the temperature defining the coldest 10 percent cloud area. The analysis further shows that the two dominant parameters in rainfall estimation are the existence of cold cloud and the duration of cloud over a point. Previously announced in STAR as N84-13735

Negri, A. J.↗

NASA NTRS1984

Nucleation of (He-3)-B from the A phase - A cosmic-ray effect?

When He-3 is liquified and subsequently cooled, it undergoes, at a temperature of approximately 2.0-2.5 mK, a second-order transition into the so-called A phase. On further cooling, a first-order transition occurs, and the liquid passes into the B phase. Supercooling occurs with respect to the second transition, and the problem of nucleation arises. In connection with the experimental interest in studying metastable (He-3)-A down to the lowest temperatures in weak magnetic fields, the understanding of the mechanism of this transition and the feasibility of its inhibition is of some importance. The present investigation is concerned with the possibility that the transition is nucleated by the passage of a cosmic ray through the sample cell.

Leggett, A. J.↗

NASA NTRS1984

An improved algorithm for evaluating trellis phase codes

Mulligan, M. G.↗

NASA NTRS1986

Comparison of stratospheric air parcel trajectories calculated from SSU and LIMS satellite data

Midstratospheric trajectories for February and March 1979 are calculated using geopotential analyses derived from limb infrared monitor of the stratosphere data. These trajectories are compared with the corresponding results using stratospheric sounding unit data. The trajectories are quasi-isentropic in that a radiation scheme is used to simply cross-isentrope flow. The results show that in disturbed conditions, quantitative agreement the trajectories, that is, within 25 great circle degrees (GCD) (one GCD about 110 km) may be valid for only 3 or 4 days, whereas during quiescent periods, quantitative agreement may last up to 10 days. By comparing trajectories calculated with different data some insight can be gained as to errors due to vertical resolution and horizontal resolution (due to infrequent sampling) in the analyzed geopotential height fields. For the disturbed trajectories described in this paper the horizontal resolution of the data was more important than vertical resolution; however, for the quiescent trajectories, which could be calculated accurately for a longer duration because of the absence of appreciable transients, the vertical resolution of the data was found to be more important than the horizontal resolution. It is speculated that these characteristics are also applicable to trajectories calculated during disturbed and quiescent periods in general. A review of some recently published trajectories shows that the qualitative conclusions of such works remains unaffected when the calculations are repeated using different data.

Austin, J.↗

NASA NTRS1989

Detecting biotic and hydrogeochemical processes in large peat basins with Landsat TM imagery

A survey was made of three large peat basins in boreal North America with Landsat TM imagery and field sampling. False-color composites composed of Bands 2, 3, and 4 are particularly effective in discriminating the major vegetation types and the important hydrogeochemical processes in these peatlands. This imagery indicates that the discharge of alkaline groundwater provides one of the most important regional and local controls on peatland development.

Glaser, Paul H.↗

NASA NTRS1993

Thermal expansion behavior of LDEF metal matrix composites

The thermal expansion behavior of Long Duration Exposure Facility (LDEF) metal matrix composite materials was studied by (1) analyzing the flight data that was recorded on orbit to determine the effects of orbital time and heating/cooling rates on the performance of the composite materials, and (2) characterizing and comparing the thermal expansion behavior of post-flight LDEF and lab-control samples. The flight data revealed that structures in space are subjected to nonuniform temperature distributions, and thermal conductivity of a material is an important factor in establishing a uniform temperature distribution and avoiding thermal distortion. The flight and laboratory data showed that both Gr/Al and Gr/Mg composites were stabilized after prolonged thermal cycling on orbit. However, Gr/Al composites showed more stable thermal expansion behavior than Gr/Mg composites and offer advantages for space structures particularly where very tight thermal stability requirements in addition to high material performance must be met.

Le, Tuyen D.↗

NASA NTRS1999

Diffusive and Radiative Transport in Fires (DARTFire): Opposed-Flow Flame Spread in Low-Velocity Flows

For flames spreading into a low-velocity flow that can only be obtained in microgravity, we have observed behavior that is different from that which is obtained at higher velocities where radiative effects are unimportant and species transport is relatively fast. Unfortunately, lack of a large body of low-gravity flame spread experimental data inhibits progress in developing an understanding of the physics of low-velocity, opposed-flow flame spread phenomena. Recent DARTFire sounding rocket experimental studies though, coupled with developing theory and modelling, have allowed some strides in understanding to be made, on which we report here. Four launches to date have resulted in six experiments for opposed-flow flame spread over a thick PMMA sample. During the 6 min microgravity period, the PMMA samples were ignited, and steady flame spread was studied under varied flow velocity, oxidizer atmospheric conditions, and, because radiative heat transfer is so important in these slowly spreading flames, external radiant flux. These were the first attempts at such experimental control and measurement in microgravity. A recent reflight of the Solid Surface Combustion Experiment (SSCE) has demonstrated, as modelling predicts, that for the thick fuel of the DARTFire experiment, flame spread in a quiescent environment is a transient process evolving from ignition to extinction on the order of 600 s (Altenkirch et al., 1999). Further study then of the effects of radiation in the very low-velocity opposing flows is of interest in understanding the transition from steady, sustained spread to the unsteady evolution to extinction as the opposing flow is reduced further and eventually removed.

Altenkirch, R. A.↗