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NASA NTRS2008

Stereoscopic Imaging in Hypersonics Boundary Layers using Planar Laser-Induced Fluorescence

Stereoscopic time-resolved visualization of three-dimensional structures in a hypersonic flow has been performed for the first time. Nitric Oxide (NO) was seeded into hypersonic boundary layer flows that were designed to transition from laminar to turbulent. A thick laser sheet illuminated and excited the NO, causing spatially-varying fluorescence. Two cameras in a stereoscopic configuration were used to image the fluorescence. The images were processed in a computer visualization environment to provide stereoscopic image pairs. Two methods were used to display these image pairs: a cross-eyed viewing method which can be viewed by naked eyes, and red/blue anaglyphs, which require viewing through red/blue glasses. The images visualized three-dimensional information that would be lost if conventional planar laser-induced fluorescence imaging had been used. Two model configurations were studied in NASA Langley Research Center's 31-Inch Mach 10 Air Wind tunnel. One model was a 10 degree half-angle wedge containing a small protuberance to force the flow to transition. The other model was a 1/3-scale, truncated Hyper-X forebody model with blowing through a series of holes to force the boundary layer flow to transition to turbulence. In the former case, low flowrates of pure NO seeded and marked the boundary layer fluid. In the latter, a trace concentration of NO was seeded into the injected N2 gas. The three-dimensional visualizations have an effective time resolution of about 500 ns, which is fast enough to freeze this hypersonic flow. The 512x512 resolution of the resulting images is much higher than high-speed laser-sheet scanning systems with similar time response, which typically measure 10-20 planes.

Danehy, Paul M.↗

NASA NTRS2009

Single Longitudinal Mode, High Repetition Rate, Q-switched Ho:YLF Laser for Remote Sensing

Ho:YLF/LuLiF lasers have specific applications for remote sensing such as wind-speed measurement and carbon dioxide (CO2) concentration measurement in the atmosphere because the operating wavelength (around 2 m) is located in the eye-safe range and can be tuned to the characteristic lines of CO2 absorption and there is strong backward scattering signal from aerosol (Mie scattering). Experimentally, a diode pumped Ho:Tm:YLF laser has been successfully used as the transmitter of coherent differential absorption lidar for the measurement of with a repetition rate of 5 Hz and pulse energy of 75 mJ [1]. For highly precise CO2 measurements with coherent detection technique, a laser with high repetition rate is required to averaging out the speckle effect [2]. In addition, laser efficiency is critically important for the air/space borne lidar applications, because of the limited power supply. A diode pumped Ho:Tm:YLF laser is difficult to efficiently operate in high repetition rate due to the large heat loading and up-conversion. However, a Tm:fiber laser pumped Ho:YLF laser with low heat loading can be operated at high repetition rates efficiently [3]. No matter whether wind-speed or carbon dioxide (CO2) concentration measurement is the goal, a Ho:YLF/LuLiF laser as the transmitter should operate in a single longitudinal mode. Injection seeding is a valid technique for a Q-switched laser to obtain single longitudinal mode operation. In this paper, we will report the new results for a single longitudinal mode, high repetition rate, Q-switched Ho:YLF laser. In order to avoid spectral hole burning and make injection seeding easier, a four mirror ring cavity is designed for single longitudinal mode, high repetition rate Q-switched Ho:YLF laser. The ramp-fire technique is chosen for injection seeding.

Bai, Yingxin↗

NASA NTRS2009

Discovery of Photon Index Saturation in the Black Hole Binary GRS 1915+105

We present a study of the correlations between spectral, timing properties and mass accretion rate observed in X-rays from the Galactic Black Hole (BH) binary GRS 1915+105 during the transition between hard and soft states. We analyze all transition episodes from this source observed with Rossi X-ray Timing Explorer (RXTE), coordinated with Ryle Radio Telescope (RT) observations. We show that broad-band energy spectra of GRS 1915+105 during all these spectral states can be adequately presented by two Bulk Motion Comptonization (BMC) components: a hard component (BMC1, photon index Gamma(sub 1) = 1.7 -- 3.0) with turnover at high energies and soft thermal component (BMC2, Gamma(sub 2) = 2.7 -- 4.2) with characteristic color temperature < or = 1 keV, and the red-skewed iron line (LAOR) component. We also present observable correlations between the index and the normalization of the disk "seed" component. The use of "seed" disk normalization, which is presumably proportional to mass accretion rate in the disk, is crucial to establish the index saturation effect during the transition to the soft state. We discovered the photon index saturation of the soft and hard spectral components at values of < or approximately equal 4.2 and 3 respectively. We present a physical model which explains the index-seed photon normalization correlations. We argue that the index saturation effect of the hard component (BMC1) is due to the soft photon Comptonization in the converging inflow close to 1311 and that of soft component is due to matter accumulation in the transition layer when mass accretion rate increases. Furthermore we demonstrate a strong correlation between equivalent width of the iron line and radio flux in GRS 1915+105. In addition to our spectral model components we also find a strong feature of "blackbody-like" bump which color temperature is about 4.5 keV in eight observations of the intermediate and soft states. We discuss a possible origin of this "blackbody-like" emission.

Titarchuk, Lev↗

NASA NTRS2009

Heavy Ion Formation in Titan's Ionosphere: Magnetospheric Introduction of Free Oxygen and a Source of Titan's Aerosols?

Discovery by Cassini's plasma instrument of heavy positive and negative ions within Titan's upper atmosphere and ionosphere has advanced our understanding of ion neutral chemistry within Titan's upper atmosphere, primarily composed of molecular nitrogen, with approx.2.5% methane. The external energy flux transforms Titan's upper atmosphere and ionosphere into a medium rich in complex hydrocarbons, nitriles and haze particles extending from the surface to 1200 km altitudes. The energy sources are solar UV, solar X-rays, Saturn's magnetospheric ions and electrons, solar wind and shocked magnetosheath ions and electrons, galactic cosmic rays (CCR) and the ablation of incident meteoritic dust from Enceladus' E-ring and interplanetary medium. Here it is proposed that the heavy atmospheric ions detected in situ by Cassini for heights >950 km, are the likely seed particles for aerosols detected by the Huygens probe for altitudes <100km. These seed particles may be in the form of polycyclic aromatic hydrocarbons (PAH) containing both carbon and hydrogen atoms CnHx. There could also be hollow shells of carbon atoms, such as C60, called fullerenes which contain no hydrogen. The fullerenes may compose a significant fraction of the seed particles with PAHs contributing the rest. As shown by Cassini, the upper atmosphere is bombarded by magnetospheric plasma composed of protons, H(2+) and water group ions. The latter provide keV oxygen, hydroxyl and water ions to Titan's upper atmosphere and can become trapped within the fullerene molecules and ions. Pickup keV N(2+), N(+) and CH(4+) can also be implanted inside of fullerenes. Attachment of oxygen ions to PAH molecules is uncertain, but following thermalization O(+) can interact with abundant CH4 contributing to the CO and CO2 observed in Titan's atmosphere. If an exogenic keV O(+) ion is implanted into the haze particles, it could become free oxygen within those aerosols that eventually fall onto Titan's surface. The process of freeing oxygen within aerosols could be driven by cosmic ray interactions with aerosols at all heights. This process could drive pre-biotic chemistry within the descending aerosols. Cosmic ray interactions with grains at the surface, including water frost depositing on grains from cryovolcanism, would further add to abundance of trapped free oxygen. Pre-biotic chemistry could arise within surface microcosms of the composite organic-ice grains, in part driven by free oxygen in the presence of organics and any heat sources, thereby raising the astrobiological potential for microscopic equivalents of Darwin's "warm ponds" on Titan.

Sittler, E. C., Jr.↗

NASA NTRS2011

Concept for Sustained Plant Production on ISS Using VEGGIE Capillary Mat Rooting System

Plant growth in microgravity presents unique challenges associated with maintaining appropriate conditions for seed germination, seedling establishment, maturation and harvest. They include maintaining appropriate soil moisture content, nutrient balance, atmospheric mixing and containment. Sustained production imposes additional challenges of harvesting, replanting, and safety. The VEGGIE is a deployable (collapsible) plant growth chamber developed as part of a NASA SBIR Phase II by Orbitec, Madison, WI. The intent of VEGGIE is to provide a low-resource system to produce fresh vegetables for the crew on long duration missions. The VEGGIE uses and LED array for lighting, an expandable bellows for containment, and a capillary matting system for nutrient and water delivery. The project evaluated a number of approaches to achieve sustained production, and repeated plantings, using the capillary rooting system. A number of different root media, seed containment, and nutrient delivery systems were evaluated and effects on seed germination and growth were evaluated. A number of issues limiting sustained production, such as accumulation of nutrients, uniform water, elevated vapor pressure deficit, and media containment were identified. A concept using pre-planted rooting packs shown to effectively address a number of those issues and is a promising approach for future development as a planting system for microgravity conditions.

Stutte, Gary W.↗

NASA NTRS2011

Exoplanetary Science: Instrumentation, Observations, and Expectations

More than 700 exoplanets have been discovered and studied using indirect techniques, leading our field into the exciting new era of comparative exoplanetology. However, the direct detection of exoplanetary systems still remains at the sensitivity limits of both ground- and space-based observatories. The development of new technologies for adaptive optics systems and high contrast instruments continues to increase the ability to directly study exoplanets. The scientific impact of these developments has promising prospects for both short and long timescales. In my talk, I will discuss recent highlights from the SEEDS survey and the current instrumentation in use at the Subaru telescope. SEEDS is a high contrast imaging strategic observing program with 120 nights of time allocated at the NAOJ's flagship optical and infrared telescope. I will also describe new instrumentation I designed to improve the SEEDS capabilities and efficiency. Finally, I will briefly discuss the conceptual design of a transiting planet camera to fly as a potential second generation instrument on-board NASA's SOFIA observatory.

McElwain, Michael↗

NASA NTRS2013

Development and Testing of a Green Monopropellant Ignition System

This paper will detail the development and testing of a "green" monopropellant booster ignition system. The proposed booster ignition technology eliminates the need for a pre-heated catalyst bed, a high wattage power source, toxic pyrophoric ignition fluids, or a bi-propellant spark ignitor. The design offers the simplicity of a monopropellant feed system features non-hazardous gaseous oxygen (GOX) as the working fluid. The approach is fundamentally different from all other "green propellant" solutions in the aerospace in the industry. Although the proposed system is more correctly a "hybrid" rocket technology, since only a single propellant feed path is required, it retains all the simple features of a monopropellant system. The technology is based on the principle of seeding an oxidizing flow with a small amount of hydrocarbon.1 The ignition is initiated electrostatically with a low-wattage inductive spark. Combustion gas byproducts from the hydrocarbon-seeding ignition process can exceed 2400 C and the high exhaust temperature ensures reliable main propellant ignition. The system design is described in detail in the Hydrocarbon-Seeded Ignition System Design subsection.

Whitmore, Stephen A.↗

NASA NTRS2014

Preparation of Metal Nanowire Decorated Carbon Allotropes

In the method of embodiments of the invention, the metal seeded carbon allotropes are reacted in solution forming zero valent metallic nanowires at the seeded sites. A polymeric passivating reagent, which selects for anisotropic growth is also used in the reaction to facilitate nanowire formation. The resulting structure resembles a porcupine, where carbon allotropes have metallic wires of nanometer dimensions that emanate from the seed sites on the carbon allotrope. These sites are populated by nanowires having approximately the same diameter as the starting nanoparticle diameter.

Southward, Robin E.↗

NASA NTRS2016

Preparation of Metal Nanowire Decorated Carbon Allotropes

Southward, Robin E.↗

NASA NTRS2017

Zinnia Germination and Lunar Soil Amendment

Germination testing was performed to determine the best method for germinating zinnias. This method will be used to attempt to germinate the zinnia seeds produced in space. It was found that seed shape may be critically important in determining whether a seed will germinate or not. The ability of compost and worm castings to remediate lunar regolith simulant for plant growth was tested. It was found that neither treatment effectively improves plant growth in lunar regolith simulant. A potential method of improving lunar regolith simulant by mixing it with arcillite was discovered.

Reese, Laura↗

NASA NTRS2018

Automated Scenario Generation for Human-in-the-Loop Simulations

Automated Multi-Aircraft Control System scenario generation for Human-in-the-Loop (HITL) evaluations of air traffic management concepts is described. The objective is to replace the difficult manual process with the automated process for creating an initial (seed) scenario that serves as a starting point for manual adjustments for creating the Human-in-the-Loop scenario. Methods for analyzing and comparing the seed-scenario generated using the automated process and the Human-in-the-Loop-scenario derived from it to meet the experiment objectives are discussed. Results of comparison of input Human-in-the-Loop-scenario with the Multi-Aircraft Control System output are also presented. The main findings are: (1) many of the characteristics of the seed-scenario used for constructing the Human-in-the-Loop-scenario are preserved in the Human-in-the-Loop-scenario, (2) landing rate profile of the traffic generated by the Multi-Aircraft Control System (MACS) using the input scenario compares reasonably well with that intended in the input scenario, and (3) many of the desired characteristics of the Human-in-the-Loop-scenario can be achieved by further automation.

Automated Scenario Generation↗

NASA NTRS2018

Mitigation of Polystyrene Microsphere Surface Contamination for Wind Tunnel Applications

Polystyrene latex (PSL) microspheres have been utilized as seed material for flow visualization in wind tunnels. However, PSL microspheres have been observed to strongly adhere to wind tunnel and model surfaces. Surface contamination on the cleaning screens that remove vorticity and provide laminar flow in the test section, is particularly problematic. Agglomeration of particles on these screens cause constriction of the airflow through the screen resulting in inconsistent airflow properties in the test section. The adhesion mechanism of PSL microspheres to wind tunnel screens and 316 stainless steel flat plates, were evaluated in a contamination apparatus where small sections of screen material were exposed to PSL-seeded airflow. Using a design of experiments (DOE) methodology airflow seeding parameters were changed to evaluate how these modifications affected the degree of surface contamination. The solution composition, comprised of ethanol and water, was determined to be the most significant factor in particle adhesion. Utilizing image analysis software, data were collected from the contaminated surfaces and incorporated to generate predictive particle contamination models. A relationship was identified between the solvent evaporation rate, and the morphology and magnitude of PSL contaminants on the test surfaces. This analysis can be extended to other solvent mixtures to provide insight into simultaneously improving wind tunnel testing capabilities while diminishing facility contamination.

Wohl, Christopher J.↗

NASA NTRS2019

The Seismic Experiment for Interior Structure (SEIS): Experiment Data Distribution

The six sensors of SEIS (The Seismic Experiment for Interior Structure) [- one of three primary instruments on NASA's Mars Lander Insight] cover a broad range of the seismic bandwidth, from 0.01 hertz to 50 hertz, with possible extension to longer periods. Data are transmitted in the form of three continuous VBB (Very Broad-Band) components at 2 samples per second (sps), an estimation of the short period (SP) energy content from the SP at 1 sps, and a continuous compound VBB/SP vertical axis at 10 sps. The continuous streams are augmented by requested event data with sample rates from 20 to 100 sps. SEIS data products are downlinked from the spacecraft in raw CCSDS (Consultative Committee for Space Data Systems) packets and converted to both the Standard for the Exchange of Earthquake Data (SEED) format files and ASCII tables (GeoCSV) for analysis and archiving. Metadata are available in dataless SEED and StionXML. Time series data (waveforms) are available in miniseed and GeoCSV. Data are distributed according to FDSN (Federation of Digital Seismograph Networks - http://www.fdsn.org) formats and interfaces. Wind, pressure and temperature data from the Auxiliary Payload Sensor Suite (APSS) will also be available in SEED format, and can be used for decorrelation and diagnostic purposes on SEIS.

Pardo, Constanza↗

NASA NTRS2019

Early Prototype Development of the Passive Orbital Nutrient Delivery System (PONDS)

Long-duration space missions will eventually require a fresh food supply to supplement crew diets, which means growing crops in space. The Passive Orbital Nutrient Delivery System (PONDS) is a new plant growth approach that contains both an area for a contained substrate and a reservoir for water and/or plant nutrient solutions. Ground studies have shown that the system facilitates both reliable water delivery to seeds for germination (e.g., while avoiding overwatering), and transport of water from the reservoir for improved plant growth while providing nutrients and oxygen to the root zone. In ground prototypes a capillary mat wicking material passively links the water/nutrient solution reservoir to a removable rooting module containing a substrate adapted to support plant growth. Oxygen permeable membranes are incorporated into both the reservoir walls and the rooting modules, bringing in oxygen from outside of the system into the reservoir and then into the rooting modules where the plant roots proliferate. Water is delivered from the reservoir to the substrate contained within the rooting module through the use of wicking material inserted into the plant growth substrate both from the bottom and from the sides of the rooting module. The capillary mat material is intrinsically hydrophilic and continuously wicks water to the substrate throughout the plant growth interval. The system is therefore self-watering in terms of supplying water to the root zone encompassed within the rooting module on demand. At the top, a hydrophilic phenolic foam plug surrounds the wick in the seed insertion zone, and both contains the substrate within the rooting module, and facilitates removal of excess moisture from the capillary mat wick before it can encompass seeds prior to germination. This work is supported by NASA’s Space Life and Physical Sciences and Research Applications Division (SLPSRAD).

Levine, Howard G.↗

NASA NTRSDate not supplied

Microgreens Food Safety Evaluation

Microgreens have been recently identified as a new type of pick and eat salad crop that can be utilized in space crop production systems. The majority of traditionally grown leafy green crops can be grown as microgreens, in addition to crops such as legumes (e.g. pea shoots), sunflower, buckwheat, most herbs, and corn, presenting hundreds of microgreen crop options. With a wide variety of flavors, exceptional nutritional density, short growth cycles (7-14 days), and unmatched volume optimization potential, microgreens present as an interesting option for sustainable production of nutritious and flavorful crops in space. The food safety aspects of microgreens have been investigated by USDA in recent years, however, an assessment for crew consumption purposes has not yet been conducted to capture the variety of microgreen cultivar types grown in spaceflight relevant environmental conditions and hardware that could be a part of the astronaut diet. Seed sanitation methods will be developed for each microgreen cultivar to sanitize the seed coat and ensure viable germination and plant growth. Food safety analysis will be performed on 24 different fast-growing cultivars of microgreens by performing aerobic plate counts (APC), fungal counts, and select pathogen screening of edible plant tissue before and after treatment with ProSan sanitizing produce wash. Additional avenues of investigation into microgreens food safety will be undertaken to understand impacts of plant height at time of harvest (able to be manipulated by light spectrum), seed density at time of planting, and role of blue light in food safety metrics of microgreens (n=6-12 microgreen cultivars tested, depending on variable). These findings will be included in the Space Crop Production Hazard Analysis Critical Control Point (HACCP) plan. This new work is currently postponed due to Covid-19.

M E Hummerick↗

NASA NTRSDate not supplied

APH Experiments: Germination and Seedling Establishment Methods

Plant experiments during ground studies were conducted in the Advanced Plant Habitat (APH) Science Carrier (SC) for ensuring high germination rates and vigorous seedling establishment. The methods constitute a mixture of environmental and planting factors that control moisture and humidity during early seedling establishment. The APH uses granular media that is fertilized with slow release fertilizer pellets. Seeds were sanitized and their germination was tested prior to use in the SC. Seeds were glued in place with guar gum in different wicking materials. It was found that seedlings establishment was reduced if the chamber was too dry or if salt buildup near the seeds was permitted. Thus, germination was carried in media that was flooded and drained, in a chamber held at 80% relative humidity at a low wind speed of 0.3 m/s. After a week the humidity was lowered to 60% RH and wind speed was increased to 0.6 m/s. The germination of different plant species were found to respond differently to germination wicks or to gauze, as well as fertilization rates. Several methods for growing Arabidopsis, wheat, radish and peppers in the APH SC will be presented

O Monje↗

NASA NTRS2016

Passive Water Assurance Delivery System

This report provides an analysis and evaluation of proposed design improvements to the internal water delivery system of NASA’s passive vegetable growing system. The VEGGIE system is designed to provide fresh vegetables and psychological benefits for astronauts aboard the International Space Station. Preliminary testing conducted by NASA for the VEGGIE system revealed flaws in the water delivery system. In these tests, the interface between the plants and the water reservoir failed to provide passive water delivery through the system both in microgravity experiments and experiments conducted on Earth. Our team’s goal was to fix this interface such that water can be passively delivered from the water reservoir to the plant rooting pillows to grow vegetative crops from seed to harvest. The system requirements outlined by NASA include: minimal total mass, on-demand passive water delivery, same dimensions as existing fixtures, minimal pressure on system to prevent leaking, zero mold growth, minimal swelling or clogging for non-flammable capillary materials, pressure stabilization between the water reservoir and plant pillow bags, even dispersal of water during initial priming, minimal bubble obstruction of capillary interface, maximum gas availability to plant roots, growth in a mixed artificial media (50:50 Arcillite:Fafard #2), and to avoid overwatering and drought conditions for plants. The major proposed design improvements are to use a single-interface capillary cord design to directly connect the water reservoir to the plant-rooting pillows, and to alter the water reservoir to model a propellant management device (PMD) in order to ensure consistent and long term watering for the VEGGIE system. The plant rooting pillow required minimal changes outside of replacing the capillary mat on the bottom of the pillow with O-ring insertion points the single-interface capillary system. - Research was conducted to determine which materials are able to uptake water through capillary action to grow Outredgeous Lettuce plants from seed to harvest. The primary requirements to be met by the team's design and evaluation of the capillary interface were: continuous passive watering for 90 days using non-flammable capillary materials and a peak water delivery rate of 30 mL/hr./0.15 m 2 . The results of the team's experimentation showed that the capillary material Nomex displayed the highest capillary water delivery potential with a maximum flow rate of 3.6 mL/hr. The Nomex capillary systems were the only capillary material to consistently grow Outredgeous Lettuce plants from seed to harvest, and displayed the highest average flow rate for multiple experiment sets. The Nomex material was previously incorporated into the VEGGIE system using a matted version of the capillary material, though the team recommends using a cord configuration in the single-interface capillary system for greater system stability. Nomex has proven to be a promising material, as it has passed both health and fire standards for use aboard the International Space Station. PMDs are made of materials that utilize surface tension and adhesive forces to overcome adverse accelerations to improve stability and ensure fluid delivery. PMDs are typically used in fuel tanks to ensure fuel delivery. The team recommends using a sponge PMD in order to mitigate bubble obstruction, decrease system weight, and ensure reliable water delivery to the capillary interface. The PMD water reservoir requires a rigid water reservoir and a vent tube for pressure stabilization. Since PMD’s cannot be tested in 1-G (Earth conditions), further theoretical modeling and testing is required for the proposed water reservoir design. The intent of this proposed system is to passively water plants in microgravity, though the technology is not limited to microgravity applications. The testing at The Ohio State University has proven that the design is highly effective on Earth, demonstrating that it could serve as a simple water delivery system in home and office applications. This would make vegetative crops more accessible in all indoor applications, thereby improving indoor air quality and occupant comfort. Additionally, this technology has great potential to be utilized in greenhouse plant production, cutting back on more sophisticated watering system energy and time requirements.

Alexandria Jensen↗

NASA NTRS2024

3D Printed Materials Characterization for Rapid Prototyping and Plant Growth

Through KSC IRTD funding in 2022, this project brought a list of 18, 3D printed filaments into formal characterization testing to provide a reference for their behaviors under relevant applications. The project format set up a series of tests to expose 3D printed specimens. A total of 1,989 individual 3D printed test specimens were sent across KSC to be scrutinized by three laboratories to fulfill a multidisciplinary assessment of each material TRL. Testing started with 18 materials. Initially, seed germination assays in the PPA, sample materials were enclosed in petri dishes with lettuce seeds on damp germination paper. No significant impacts on lettuce seed germination were observed in this testing. Next, sample coupons were printed and sent for materials testing to the KSC Analysis/Mechanical and Environmental Testing Laboratory, where they were subjected to 14- and 30-day soak periods in solutions used to provide nutrients to plants or to sanitize hardware before and after use. Following a long soak typical of a 30-day plant growout in Hoagland’s solution, 14 materials gained more than 10% of their own mass. This indicated an increased potential for leaching or providing conditions that are not food safe. Materials that exceeded 15% absorption by mass were eliminated from further testing. Based off this result, the team continued with a core list of nine filaments to fulfill Tensile, Flexural, Biofilm formation, and plant growth testing. Those materials were PLA (Raise3D), ABS (Raise3D), PETG (PolyethyleneTerephthalate Glycol) (Raise3D), ASA (Acrylonitrile Styrene Acrylate) (Raise3D), PC (Polycarbonate) (Raise3D), TPU (Thermoplastic polyurethane)-95 (Raise3D), PLA Copper (Gizmodorks), PP (Polypropylene) (Braskem), and HIPS (High Impact Polystyrene) (Gizmodorks). Testing also quantified the spectral impact of using different color 3D printed surfaces in a growth chamber. The material used for spectral testing was PLA. Printing employed a standard surface texture representative of all materials. It was shown through Tensile Testing (ASTM D638-22) that the breaking force of a 3D printed part greatly varied depending on layer orientation. This is common through all materials, and demonstrates that the strength of a 3D printed component can be maximized by layering the material normal to the primary force on the part. Four-point flexural testing (ASTM D790) provided quantities of interest, Flexural modulus, Flexural strength, Flexural stress, and strain at break within a 5% strain limit from each of nine materials. Biofilm formation testing was conducted in the Molecular and Microbiological Laboratory. Testing completed on specimens from each material showed equal formation on the surface. Additional plant growth testing was conducted in the PPA beyond the initial germination testing. The final assessment documents that three materials (PLA, ABS, and PC) have reached TRL 6 through extensive testing, and ultimate end-to-end applied use in experimental or testing conditions (flight and ground). TRL 5 materials (ASA, TPU-95, PLA Copper, PP, PETG, and HIPS) have all been successfully applied in Research and Development for crop growth applications and are ready to be applied in formal testing. TRL 4 materials Nylon910, PLA Carbon Fiber, PPA CF, PPA Glass Fiber (GF), NinjaFlex, and P-filament 721 are materials that were able to be printed and tested, but have yet to show data meeting applied requirements. TRL 3 NylonX, Flex TPE-185, and Nylon were unable to be reliably printed to fulfill testing. These results provide researchers with reference for materials to use during plant growth experimentation, and also set a standard for future characterization work applying 3D printing and materials to testing, research, and experimentation.

Gioia Massa↗