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At least 1,567 records · Page 87

Increasing thermostability of the key photorespiratory enzyme glycerate 3‐kinase by structure‐based recombination

As global temperatures rise, improving crop yields will require enhancing the thermotolerance of crops. One approach for improving thermotolerance is using bioengineering to increase the thermostability of enzymes catalysing essential biological processes. Photorespiration is an essential recycling process in plants that is integral to photosynthesis and crop growth. The enzymes of photorespiration are targets for enhancing plant thermotolerance as this pathway limits carbon fixation at elevated temperatures. We explored the effects of temperature on the activity of the photorespiratory enzyme glycerate kinase (GLYK) from various organisms and the homologue from the thermophilic alga Cyanidioschyzon merolae was more thermotolerant than those from mesophilic plants, including Arabidopsis thaliana. To understand enzyme features underlying the thermotolerance of C. merolae GLYK (CmGLYK), we performed molecular dynamics simulations using AlphaFold-predicted structures, which revealed greater movement of loop regions of mesophilic plant GLYKs at higher temperatures compared to CmGLYK. Based on these simulations, hybrid proteins were produced and analysed. These hybrid enzymes contained loop regions from CmGLYK replacing the most mobile corresponding loops of AtGLYK. Two of these hybrid enzymes had enhanced thermostability, with melting temperatures increased by 6 °C. One hybrid with three grafted loops maintained higher activity at elevated temperatures. Whilst this hybrid enzyme exhibited enhanced thermostability and a similar Km for ATP compared to AtGLYK, its Km for glycerate increased threefold. This study demonstrates that molecular dynamics simulation-guided structure-based recombination offers a promising strategy for enhancing the thermostability of other plant enzymes with possible application to increasing the thermotolerance of plants under warming climates.

59 BASIC BIOLOGICAL SCIENCES↗

The structure of the RCAN1:CN complex explains the inhibition of and substrate recruitment by calcineurin

Regulator of calcineurin 1 (RCAN1) is an endogenous inhibitor of the Ser/Thr phosphatase calcineurin (CN). It has been shown that excessive inhibition of CN is a critical factor for Down syndrome and Alzheimer’s disease. Here, we determined RCAN1’s mode of action. Using a combination of structural, biophysical, and biochemical studies, we show that RCAN1 inhibits CN via multiple routes: first, by blocking essential substrate recruitment sites and, second, by blocking the CN active site using two distinct mechanisms. We also show that phosphorylation either inhibits RCAN1-CN assembly or converts RCAN1 into a weak inhibitor, which can be reversed by CN via dephosphorylation. This highlights the interplay between posttranslational modifications in regulating CN activity. Last, this work advances our understanding of how active site inhibition of CN can be achieved in a highly specific manner. Together, these data provide the necessary road map for targeting multiple neurological disorders.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Measurement of neutral current single π 0 production on argon with the MicroBooNE detector

We report the first measurement of π 0 production in neutral current (NC) interactions on argon with average neutrino energy of ≲1 GeV. We use data from the MicroBooNE detector’s 85-tonne active volume liquid argon time projection chamber situated in Fermilab’s Booster Neutrino Beam and exposed to 5.89 × 10 20 protons on target for this measurement. Measurements of NC π 0 events are reported for two exclusive event topologies without charged pions. Those include a topology with two photons from the decay of the π 0 and one proton and a topology with two photons and zero protons. Flux-averaged cross-sections for each exclusive topology and for their semi-inclusive combination are extracted (efficiency-correcting for two-plus proton final states), and the results are compared to predictions from the GENIE, NEUT, and NUWRO neutrino event generators. We measure cross sections of 1.243 ± 0.185 (syst) ±0.076 (stat), 0.444 ± 0.098 ± 0.047, and 0.624 ± 0.131 ± 0.075 [10 -38 cm 2 /Ar] for the semi-inclusive NCπ 0 , exclusive NCπ 0 +1p, and exclusive NCπ 0 +0p processes, respectively.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Q4Q: Quantum Computation for Quantum Prediction of Materials and Molecular Properties

We aim at exploring the potential of quantum computation to solve practical problems that are currently targeted by “traditional” high performance computing (HPC). Along the way, we will develop theoretical frameworks and algorithmic strategies. We propose computational research activities that will advance the application of quantum computers to selected materials/molecular properties: (1) Band structure of solids; (2) Molecular electronic/vibronic properties; (3) Free energies and phase transitions.

36 MATERIALS SCIENCE↗

Novel methods for TH-229 production through fast neutron irradiation of TH-23F0 and charged particle irradiation of Th-230 and Th-232. Final Report DE-SC0020140

The activities and results of research related to accelerator production of Pa and Th isotopes via bombardment of 230 Th and 232 Th targets are described herein. This research was supported through DOE grant DE-SC0020140 and was conducted in collaboration with Oak Ridge National Laboratory and Lawrence Berkeley National Laboratory. Cross section measurements, transport model and nuclear physics model calculations are presented. Highlights include the first ever measurements of 229 Pa, two publications, and graduation of a PhD student.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

The Johns Hopkins Ultraviolet Telescope for Shuttle astronomy

The Hopkins Ultraviolet Telescope (HUT) is a 90-cm f/2 prime focus telescope and spectrometer with a photon-counting microchannel plate detector designed to obtain moderate resolution (wavelength interval, approximately 4 A) spectrophotometry of faint (visual magnitude, less than approximately 17) objects in the far-ultraviolet spectral region (approximately 900-1700 A). The HUT will be mounted on the Spacelab Instrument Pointing System and will be operated by a Payload Specialist using a field acquisition TV system to assist in locating targets. The initial flight of the HUT, expected in 1984 or 1985, will be devoted primarily to the study of quasars and active galactic nuclei, emphasizing the 900-1200 A spectral region which is inaccessible with other existing or currently planned orbiting instruments.

Davidson, A. F.↗

Radio stimulation and diagnostics of space plasmas

We have investigated the small-scale topside ionospheric plasma structures first observed at Millstone Hill, Massachusetts with the 440 MHz incoherent scatter radar. These small-scale obliquely propagating plasma modes occurring in the vicinity of the midlatitude ionospheric trough, have large radar cross-sections and narrow spectral widths. They have, until recently, been dismissed solely as hard target contamination of the incoherent scatter radar. The geophysical conditions associated with the ionospheric trough, such as the field-aligned current activity and steep plasma density gradients, suggest that these recently discovered small-scale topside ionospheric plasmas may also appear in the auroral and polar ionosphere. In fact, this speculation has been corroborated by the preliminary experiments and data analyses at Tromso, Norway and Sondrestromfjord, Greenland. The primary research results are highlighted. Described in Section 3 are the experiments conducted at Arecibo, Puerto Rico in the past summer for simulating the geophysical conditions of generating these topside ionospheric plasma structures. Recommendation for the future research is finally given. Attached as the appendix of this report are several chapters which present the detailed results of research in the concerned topside ionospheric clutter. Highlights of the research results include: (1) causes of the enhanced radar backscatter (ERB) phenomenon; (2) occurrence of the ERB phenomenon; (3) altitudes of the ERB phenomenon; (4) strength of the ERB returns; (5) range of altitudes of the ERB returns; (6) occurrence frequency of the ERB phenomenon; (7) Doppler effect of the ERB phenomenon; (8) persistency of the ERB; and (9) distinction between ERB phenomenon and space object signatures.

Lee, Min-Chang↗

MARSNET: A European network of stations on the surface of Mars

Following an ESA preliminary study on the possible areas of European participation in the future international exploration of Mars and an ESA call for ideas of new missions, MARSNET, a network of small surface stations, was selected for further in-depth scientific and technical assessment studies as a potential European contribution to such exploration. Subsequently, the MARSNET phase A studies started in the autumn of 1991. The industrial kickoff took place in early January 1992, following the tender evaluation and the decision to select the Aerospatiale-led consortium including Dornier, Alcatel, Laben, and Etca to perform the industrial studies. The phase A studies ended in early 1993. However, critical items such as an instrument deployment device continue to be studied in the framework of ESA's Technology Research Program. The MARSNET mission consists of a network of three semihard landers to be placed on the Martian surface, several thousand kilometers apart, thus defining a regional/global seismological and meteorological network in the Tharsis region. The small stations would be targeted for landing at scientifically interesting sites in this region of Mars, which is the most likely area to still show tectonic activity; this would allow the seismometers to acquire data for the determination of the internal structure of the planet. Landing site geology and geochemistry will also be studied.

Chicarro, A. F.↗

The ATIC Long Duration Balloon Project

Long Duration Balloon (LDB) scientific experiments, launched to circumnavigate the south pole over Antarctica, have particular advantages compared to Shuttle or other Low Earth Orbit (LEO) missions in terms of cost, weight, scientific 'duty factor' and work force development. The Advanced Thin Ionization Calorimeter (ATIC) cosmic ray astrophysics experiment is a good example of a university-based project that takes full advantage of current LDB capability. The ATIC experiment is currently being prepared for its first LDB science flight that will investigate the charge composition and energy spectra of primary cosmic rays over the energy range from about 10(exp 10) to 10(exp 14) eV. The instrument is built around a fully active, Bismuth Germanate (BGO) ionization calorimeter to measure the energy deposited by the cascades formed by particles interacting in a thick carbon target. A highly segmented silicon matrix, located above the target, provides good incident charge resolution plus rejection of the 'backscattered' particles from the interaction. Trajectory reconstruction is based on the cascade profile in the BGO calorimeter, plus information from the three pairs of scintillator hodoscope layers in the target section above it. A full evaluation of the experiment was performed during a test flight occurring between 28 December 2000 and 13 January 2001 where ATIC was carried to an altitude of approx. 37 km above Antarctica by an approx. 850,000 cu m helium filled balloon for one circumnavigation of the continent. All systems behaved well, the detectors performed as expected, more than 43 gigabytes of engineering and cosmic ray event data was returned and these data are now undergoing preliminary data analysis. During the coming 2002-2003 Antarctica summer season, we are preparing for a ATIC science flight with approx. 15 to 30 days of continuous data collection in the near-space environment of LDB float altitudes.

Guzik, T. G.↗

High-Performance, Radiation-Hardened Electronics for Space and Lunar Environments

The Radiation Hardened Electronics for Space Environments (RHESE) project develops advanced technologies needed for high performance electronic devices that will be capable of operating within the demanding radiation and thermal extremes of the space, lunar, and Martian environment. The technologies developed under this project enhance and enable avionics within multiple mission elements of NASA's Vision for Space Exploration. including the Constellation program's Orion Crew Exploration Vehicle. the Lunar Lander project, Lunar Outpost elements, and Extra Vehicular Activity (EVA) elements. This paper provides an overview of the RHESE project and its multiple task tasks, their technical approaches, and their targeted benefits as applied to NASA missions.

Keys, Andrew S.↗

Earth Knowledge Acquired by Middle School Students

Earth Knowledge Acquired by Middle School Students (EarthKAM), an education activity, allows middle school students to program a digital camera on board the International Space Station to photograph a variety of geographical targets for study in the classroom. Photos are made available on the web for viewing and study by participating schools around the world. Educators use the images for projects involving Earth Science, geography, physics, and social science.

Ride, Sally↗

Grid Visualization Tool

The Grid Visualization Tool (GVT) is a computer program for displaying the path of a mobile robotic explorer (rover) on a terrain map. The GVT reads a map-data file in either portable graymap (PGM) or portable pixmap (PPM) format, representing a gray-scale or color map image, respectively. The GVT also accepts input from path-planning and activity-planning software. From these inputs, the GVT generates a map overlaid with one or more rover path(s), waypoints, locations of targets to be explored, and/or target-status information (indicating success or failure in exploring each target). The display can also indicate different types of paths or path segments, such as the path actually traveled versus a planned path or the path traveled to the present position versus planned future movement along a path. The program provides for updating of the display in real time to facilitate visualization of progress. The size of the display and the map scale can be changed as desired by the user. The GVT was written in the C++ language using the Open Graphics Library (OpenGL) software. It has been compiled for both Sun Solaris and Linux operating systems.

Chouinard, Caroline↗

A Survey of LIDAR Technology and Its Use in Spacecraft Relative Navigation

This paper provides a survey of modern LIght Detection And Ranging (LIDAR) sensors from a perspective of how they can be used for spacecraft relative navigation. In addition to LIDAR technology commonly used in space applications today (e.g. scanning, flash), this paper reviews emerging LIDAR technologies gaining traction in other non-aerospace fields. The discussion will include an overview of sensor operating principles and specific pros/cons for each type of LIDAR. This paper provides a comprehensive review of LIDAR technology as applied specifically to spacecraft relative navigation. HE problem of orbital rendezvous and docking has been a consistent challenge for complex space missions since before the Gemini 8 spacecraft performed the first successful on-orbit docking of two spacecraft in 1966. Over the years, a great deal of effort has been devoted to advancing technology associated with all aspects of the rendezvous, proximity operations, and docking (RPOD) flight phase. After years of perfecting the art of crewed rendezvous with the Gemini, Apollo, and Space Shuttle programs, NASA began investigating the problem of autonomous rendezvous and docking (AR&D) to support a host of different mission applications. Some of these applications include autonomous resupply of the International Space Station (ISS), robotic servicing/refueling of existing orbital assets, and on-orbit assembly.1 The push towards a robust AR&D capability has led to an intensified interest in a number of different sensors capable of providing insight into the relative state of two spacecraft. The present work focuses on exploring the state-of-the-art in one of these sensors - LIght Detection And Ranging (LIDAR) sensors. It should be noted that the military community frequently uses the acronym LADAR (LAser Detection And Ranging) to refer to what this paper calls LIDARs. A LIDAR is an active remote sensing device that is typically used in space applications to obtain the range to one or more points on a target spacecraft. As the name suggests, LIDAR sensors use light (typically a laser) to illuminate the target and measure the time it takes for the emitted signal to return to the sensor. Because the light must travel from the source, to

Christian, John A.↗

Mapping the Gas Turbulence in the Coma Cluster: Predictions for Astro-H

Astro-H will be able for the first time to map gas velocities and detect turbulence in galaxy clusters. One of the best targets for turbulence studies is the Coma cluster, due to its proximity, absence of a cool core, and lack of a central active galactic nucleus. To determine what constraints Astro-H will be able to place on the Coma velocity field, we construct simulated maps of the projected gas velocity and compute the second-order structure function, an analog of the velocity power spectrum. We vary the injection scale, dissipation scale, slope, and normalization of the turbulent power spectrum, and apply measurement errors and finite sampling to the velocity field. We find that even with sparse coverage of the cluster, Astro-H will be able to measure the Mach number and the injection scale of the turbulent power spectrum-the quantities determining the energy flux down the turbulent cascade and the diffusion rate for everything that is advected by the gas (metals, cosmic rays, etc.). Astro-H will not be sensitive to the dissipation scale or the slope of the power spectrum in its inertial range, unless they are outside physically motivated intervals. We give the expected confidence intervals for the injection scale and the normalization of the power spectrum for a number of possible pointing configurations, combining the structure function and velocity dispersion data. Importantly, we also determine that measurement errors on the line shift will bias the velocity structure function upward, and show how to correct this bias.

clusters: intracluster medium↗

Mitigation of Backgrounds from Cosmogenic $^{137}$Xe in Xenon Gas Experiments using $^{3}$He Neutron Capture

\Xe{136} is used as the target medium for many experiments searching for \bbnonu. Despite underground operation, cosmic muons that reach the laboratory can produce spallation neutrons causing activation of detector materials. A potential background that is difficult to veto using muon tagging comes in the form of \Xe{137} created by the capture of neutrons on \Xe{136}. This isotope decays via beta decay with a half-life of 3.8 minutes and a \Qb\ of $\sim$4.16 MeV. This work proposes and explores the concept of adding a small percentage of \He{3} to xenon as a means to capture thermal neutrons and reduce the number of activations in the detector volume. When using this technique we find the contamination from \Xe{137} activation can be reduced to negligible levels in tonne and multi-tonne scale high pressure gas xenon neutrinoless double beta decay experiments running at any depth in an underground laboratory.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Progress on Pu-238 Production at Idaho National Laboratory From March 2021 to February 2022

Idaho National Laboratory has completed irradiation of Np-237 targets in the Advanced Test Reactor’s (ATR) South Flux Trap (SFT) and I-7 positions. INL also progressed qualification of new ATR Gen 1 Np-237 targets for the North East Flux Trap (NEFT), inner A, and H positions. This paper gives an overview of operational and technical activities from March 2021 to February 2022.

07 ISOTOPE AND RADIATION SOURCES↗

Development of Analysis Methods that Integrate Numeric and Textual Equipment Reliability Data

Within the Light Water Reactor Sustainability (LWRS) program, the Risk-Informed Systems Analysis (RISA) Pathway is performing collaborative research on the development and deployment of technologies designed to assist operating nuclear power plants (NPPs) to reduce operating costs improve plant reliability and availability. One of the RISA research areas is focusing on the development of methods and tools designed to optimize plant operations (e.g., maintenance/replacement schedules, optimal maintenance postures for plant structures, systems, and components [SSCs]) in a manner that is more cost effective than current approaches and makes better use of available SSC health data. The Risk-Informed Asset Management (RIAM) project targets this research area by creating a direct bridge between component equipment reliability (ER) data and system engineer decision making regarding maintenance activity scheduling and component aging management. In this respect, one challenge that NPP system engineers are facing is that the amount of ER data being continuously generated is not only extremely large in size, but it comes in different forms: textual (e.g., condition or maintenance reports) and numeric (e.g., generated by monitoring systems). All these data elements provide them with valuable insights and information regarding: 1) the discovery of anomalous behaviors or degradation trends, 2) the identification of the possible causes behind such behaviors/trends, and 3) the prediction of their direct consequences. However, several challenges have proved to be roadblocks to this process. While some of these challenges are technical in nature (i.e., data are often distributed over several physical servers/databases), others are conceptual in nature: data elements come in different formats (e.g., numeric or textual), and measured values have different scales (e.g., vibration spectra and oil temperature). The activities performed by the RIAM project during FY23 directly tackles the need to simultaneously integrate the analysis of ER data in all its forms, numeric and textual. Note that such task has never been performed before due to the complexity of the systems under consideration but, most importantly, because of the technical challenges behind the harmonization of ER data formats and the lack of adequate computational methods to analyze them. Our approach borrows ideas and concepts from the medical field where integration of several data sources is vital to assist medical practitioners to perform correct diagnosis and indicate optimal treatments. In our view a NPP asset is equivalent to a patient in a medical context. The main difference is the complexity of a human body is a magnitude more complex when compared to typical assets commonly present in NPPs (e.g., centrifugal pumps, or motor operated valves). This simplifies our first requirement when analyzing heterogenous ER data formats: to put data into “context”. Context is here intended as the additional piece of information that is needed by ER data analysis tools to understand what these data elements are referring to, i.e., which king of knowledge they are generating. In our context, this knowledge can be translated into models that capture the form and functional architecture of assets/systems, their dependencies, and how they interact. These models actually emulate the knowledge that that NPP system engineers possess about assets and systems; this is their key of success when analyzing ER data, their challenge is ability to handle large amount of data. Here, we employ model-based system engineering (MBSE) models of systems and assets to represent and capture their architecture and functional, i.e. cause-effect, relations. Then, ER data elements are processed by identifying first of all which elements of the developed MBSE elements they are referring to. For numeric ER data this task is fairly easy since it is possible to precisely pinpoint what MBSE elements the corresponding sensor are observing (e.g., bearing temperature of a centrifugal pump). Task is much harder for textual data since the information contained in issue or maintenance reports needs to “be understood” by a computational tool. Here we called this process as “knowledge extraction”. Once again, we borrow the experience in the medical field where methods to extract knowledge from textual data have been developed in the past decade. The missing element for us is the availability of a complete dictionary of NPP related concepts (in addition to the MBSE models presented earlier) that can put “text into context”. In FY23, such dictionary has been developed along with all the computational elements required for knowledge extraction. Lastly, once numeric and textual ER data elements have been processed and “understood”, then the last step is the discovery of possible cause-effect relations among them. This is performed by observing if a logical connection through the MBSE models exists, and if the

97 MATHEMATICS AND COMPUTING↗

In Vitro Selection of Antibodies Targeting Yersinia pestis Membrane Lipids Using Nanodisc-Based Antigen Presentation

Proteins are the most common targets for antibody discovery and vaccine development, but their sequence variability can limit the breadth of resulting antigens. Lipids represent an alternative class of antigens due to their structural conservation and roles in host–pathogen interactions. Here, we describe the development and optimization of an in vitro antibody selection workflow using lipid-containing nanodiscs as antigen presentation platforms to enable phage and yeast display selections under conditions adapted for these non-protein targets. Lipopolysaccharide (LPS) nanodiscs were first used as a model system to evaluate selection strategies, including competitive and subtractive approaches to reduce non-specific binders, yielding peptide and single-chain variable fragment (scFv) binders that were affinity matured to improve binding signals. The same approach was subsequently used to select scFv antibodies that recognize lipid nanodiscs prepared from Yersinia pestis membrane lipid extracts. These antibodies show binding to lipid nanodiscs derived from Y. pestis, with evidence of selectivity relative to control nanodiscs. Overall, this work establishes a workflow for antibody selection against lipid-containing nanodisc antigens and highlights practical considerations associated with these targets. The approach may be useful for generating affinity reagents to membrane-associated lipids, although further characterization is required to define antigen specificity and functional activity.

59 BASIC BIOLOGICAL SCIENCES↗