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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 1,531 records · Page 85
All sourcesNASA NTRSDOE OSTI
DOE OSTI2014

Synthesis and characterization of antiperovskite nitrides GaNCr{sub 3−x}Mn{sub x}

The effects of Mn-doping on the crystal structure, magnetic and electrical/thermal transport properties of GaNCr{sub 3−x}Mn{sub x} (0≤x≤1.5) have been investigated systematically. As a result, the lattice constant and the residual resistivity increase, while the residual resistivity ratio, electron thermal conductivity, and room-temperature carrier concentration decrease with increasing Mn-doping level. The ground state of parent compound GaNCr{sub 3} is nonmagnetic, interestingly, the ferrimagnetism and antiferromagnetism are observed in Mn-doped samples GaNCr{sub 3−x}Mn{sub x}. Correspondingly, around the antiferromagnetic transition of GaNCr{sub 3−x}Mn{sub x} the correlation effect is studied. Furthermore, the analysis of thermal conductivity data suggests that the electron thermal conductivity plays a major role in total thermal conductivity of GaNCr{sub 3} at low temperatures, while the phonon thermal conductivity is dominant for Mn-doped GaNCr{sub 3} in the whole temperature of 5–330 K. The positive values of Seebeck coefficient and Hall coefficient indicate that the nature of charge carrier is hole-type in GaNCr{sub 3−x}Mn{sub x}. - Graphical abstract: The Rietveld refinement and structural properties of GaNCr{sub 3−x}Mn{sub x}. Display Omitted - Highlights: • Polycrystalline GaNCr{sub 3−x}Mn{sub x} are synthesized by using the solid state reaction method. • A good quality of samples is verified by Rietveld refinement and RRR values. • Antiferromagnetic and ferromagnetic competitions are induced by Mn-doping. • We present a comprehensive understanding of physical properties of GaNCr{sub 3−x}Mn{sub x}.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2018

A novel IMRT planning study by using the fixed-jaw method in the treatment of peripheral lung cancer with mediastinal lymph node metastasis

Intensity-modulated radiotherapy (IMRT) is an important technology in cancer radiotherapy. In the current planning system, such as in the Pinnacle{sup 3} system, jaw positions are automatically set to cover all target volumes, and many medical centers in developing countries are not equipped with linear accelerators with a jaw tracing function. As solitary lesions are often located in patients, the resulting radiation leakage and transmission increase the dose exposure in surrounding critical organs, although blocked by multileaf collimator (MLC) leaves. We therefore designed a method to manually fix jaw positions, which further reduces doses. We particularly focused on the patients of peripheral lung cancer combined with mediastinal lymph node metastasis, as our medical center mainly targets lung cancer. We designed 2 treatment plans for each patient with the same optimization parameters, i.e., the plan of automatically chosen jaw positions (jaw auto-chosen plan) and the plan of fixed-jaw positions (fixed-jaw plan). In the latter plan, jaws were manually fixed for tumors in lung and in mediastinal lymph node metastases, respectively. We found that both plans met the clinical requirements, and the D{sub 2}, D{sub 98}, conformation number (CN), and homogeneity index (HI) for planning target volume (PTV) had no significant differences between the 2 plans. Importantly, the machine units (MUs) for fixed-jaw plans were 50%~60% more than routine jaw auto-chosen plans, whereas the V{sub 5}, V{sub 10}, V{sub 20}, V{sub 30}, and the mean dose in the total lung and the ipsilateral lung were less than the routine jaw auto-chosen plans. Dose-volume values D{sub 1} for the spinal cord and D{sub 2}, V{sub 40}, V{sub 60} for the heart existed no significant differences for 2 plans. In the fixed-jaw method, the total lung TLV{sub 5}-â–³V{sub ab} and TLV{sub 10}-â–³V{sub ab} values had a moderate positive correlation with the lung radiation leakage and the transmission area reduction. We concluded that the fixed-jaw plan is superior to the routine jaw auto-chosen plan in reducing the radiation exposure of surrounding critical organs, which will benefit the IMRT application.

62 RADIOLOGY AND NUCLEAR MEDICINE↗
DOE OSTI2015

Changes in cholesterol homeostasis and acute phase response link pulmonary exposure to multi-walled carbon nanotubes to risk of cardiovascular disease

Adverse lung effects following pulmonary exposure to multi-walled carbon nanotubes (MWCNTs) are well documented in rodents. However, systemic effects are less understood. Epidemiological studies have shown increased cardiovascular disease risk after pulmonary exposure to airborne particles, which has led to concerns that inhalation exposure to MWCNTs might pose similar risks. We analyzed parameters related to cardiovascular disease, including plasma acute phase response (APR) proteins and plasma lipids, in female C57BL/6 mice exposed to a single intratracheal instillation of 0, 18, 54 or 162 μg/mouse of small, entangled (CNT{sub Small}, 0.8 ± 0.1 μm long) or large, thick MWCNTs (CNT{sub Large}, 4 ± 0.4 μm long). Liver tissues and plasma were harvested 1, 3 and 28 days post-exposure. In addition, global hepatic gene expression, hepatic cholesterol content and liver histology were used to assess hepatic effects. The two MWCNTs induced similar systemic responses despite their different physicochemical properties. APR proteins SAA3 and haptoglobin, plasma total cholesterol and low-density/very low-density lipoprotein were significantly increased following exposure to either MWCNTs. Plasma SAA3 levels correlated strongly with pulmonary Saa3 levels. Analysis of global gene expression revealed perturbation of the same biological processes and pathways in liver, including the HMG-CoA reductase pathway. Both MWCNTs induced similar histological hepatic changes, with a tendency towards greater response following CNT{sub Large} exposure. Overall, we show that pulmonary exposure to two different MWCNTs induces similar systemic and hepatic responses, including changes in plasma APR, lipid composition, hepatic gene expression and liver morphology. The results link pulmonary exposure to MWCNTs with risk of cardiovascular disease. - Highlights: • Systemic and hepatic alterations were evaluated in female mice following MWCNT instillation. • Despite being physicochemically different, the two MWCNTs induced a similar systemic acute phase response. • We found a close correlation between plasma SAA levels and pulmonary Saa3 transcription levels. • Similar changes in plasma lipid levels and in the hepatic transcriptome were observed. • Our results indicate a link between pulmonary exposure to MWCNTs with risk of cardiovascular disease.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2016

Relationship of antioxidant and oxidative stress markers in different organs following copper toxicity in a rat model

Copper (Cu) at a higher level becomes toxic and it can catalyze the formation of highly reactive hydroxyl radical. We report the vulnerability of liver, kidney and brain to different dose of copper sulfate (CuSO{sub 4}) induced oxidative stress at different time duration. Fifty-four male Wistar rats (weight range = 205 ± 10 g) were equally divided into three groups. CuSO{sub 4} was administered orally to the experimental groups (Group-II and III) up to 90 days in a dose of 100 and 200 mg/Kg body weight per day. Saline water was given to the control group (Group-I). At the end of 30, 60 and 90 days of administration, neurobehavioral studies were done and six rats from each group were sacrificed. Their liver, kidney and brain tissues were subjected for Cu, glutathione (GSH), malondialdehyde (MDA) and total antioxidant capacity (TAC) assay. Blood urea nitrogen (BUN), serum creatinine, bilirubin and transaminases were measured. GSH, TAC and MDA levels were correlated with the markers of respective organ dysfunction. Administration of CuSO{sub 4} resulted in increased free Cu and MDA level, and decrease GSH and TAC levels in group-II and III compared with group-I. In experimental groups, the reduction in TAC and GSH levels was maximum in liver tissue followed by brain and kidney; whereas increase in MDA level was highest in liver followed by brain and kidney at 30, 60 and 90 days. TAC and GSH levels in the liver inversely correlated with serum transaminases and bilirubin, and tissue free Cu, and positively correlated with MDA levels. Free Cu level in kidney tissue and BUN inversely correlated with TAC and GSH, and positively with MDA level. Grip-strength, rotarod and Y-maze findings were inversely correlated with brain free Cu and MDA levels and positively with GSH and TAC levels. The oxidative stress was highest in liver followed by brain and kidney after oral CuSO{sub 4} exposure in a rat model. These levels correlated with the respective organ dysfunction and tissue free Cu concentration. - Highlights: • Oral dosing of CuSO{sub 4} leads to oxidative stress in liver, brain and kidney. • Liver has maximum oxidative stress followed by brain and kidney. • Oxidative stress correlated with the respective organ dysfunction and tissue Cu concentration.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2018

Spatial-temporal variations of dissolved organic nitrogen molecular composition in agricultural runoff water

Leaching of dissolved organic nitrogen (DON) has been reported as a pathway of N loss from agriculture, but the molecular composition of DON in agricultural water is poorly understood. Runoff water samples were collected from citrus grove furrows (CGF), ditches (CGD) and pasture ditches (PD) in four seasons. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) was used to investigate molecular composition of DON. Chemodiversity index of DON had spatiotemporal variations, while the molecular composition of total DON showed minimal variations, except for PD in November. Lignin derivatives constituted 61% of the total DON compounds. Relative abundance of aliphatic compounds, char and condensed aromatics of unique DON compounds varied spatiotemporally and had a significant correlation with DON concentration. Aromaticity index decreased from CGF to connected CGD, implying that photodegradation is possibly the dominant process that alters molecular composition of aquatic DON during the transport. Significant differences in unique DON composition between CGD and PD indicates that fertilization and land use affect DON composition. The information on molecular characterization of DON should be useful for tracking DON source and developing technologies to remove DON in the agricultural runoff water.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2015

Oxygen-dependent acetylation and dimerization of the corepressor CtBP2 in neural stem cells

The transcriptional corepressor CtBP2 is essential for proper development of the nervous system. The factor exerts its repression by interacting in complexes with chromatin-modifying factors such as histone deacetylases (HDAC) 1/2 and the histone demethylase LSD1/KDM1. Notably, the histone acetyl transferase p300 acetylates CtBP2 and this is an important regulatory event of the activity and subcellular localization of the protein. We recently demonstrated an essential role for CtBPs as sensors of microenvironmental oxygen levels influencing the differentiation potential of neural stem cells (NSCs), but it is not known whether oxygen levels influence the acetylation levels of CtBP factors. Here we show by using proximity ligation assay (PLA) that CtBP2 acetylation levels increased significantly in undifferentiated, proliferating NSCs under hypoxic conditions. CtBP2 interacted with the class III HDAC Sirt1 but this interaction was unaltered in hypoxic conditions, and treatment with the Sirt1 inhibitor Ex527 did not result in any significant change in total CtBP2 acetylation levels. Instead, we revealed a significant decrease in PLA signal representing CtBP2 dimerization in NSCs under hypoxic conditions, negatively correlating with the acetylation levels. Our results suggest that microenvironmental oxygen levels influence the dimerization and acetylation levels, and thereby the activity, of CtBP2 in proliferating NSCs.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2016

Probing electronic wave functions of sodium-doped clusters: Dyson orbitals, anisotropy parameters, and ionization cross-sections

We apply high-level ab initio methods to describe the electronic structure of small clusters of ammonia and dimethylether (DME) doped with sodium, which provide a model for solvated electrons. We investigate the effect of the solvent and cluster size on the electronic states. We consider both energies and properties, with a focus on the shape of the electronic wave function and the related experimental observables such as photoelectron angular distributions. The central quantity in modeling photoionization experiments is the Dyson orbital, which describes the difference between the initial N-electron and final (N-1)-electron states of a system. Dyson orbitals enter the expression of the photoelectron matrix element, which determines total and partial photoionization cross-sections. We compute Dyson orbitals for the Na(NH3)n and Na(DME)m clusters using correlated wave functions (obtained with equation-of-motion coupled-cluster model for electron attachment with single and double substitutions) and compare them with more approximate Hartree-Fock and Kohn-Sham orbitals. As a result, we also analyze the effect of correlation and basis sets on the shapes of Dyson orbitals and the experimental observables.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2020

Geodetic Measurements of Slow-Slip Events Southeast of Parkfield, CA

Tremor and low-frequency earthquakes are presumed to be indicative of surrounding slow, aseismic slip that is often below geodetic detection thresholds. This study uses data from borehole seismometers and long-baseline laser strainmeters to observe both the seismic and geodetic signatures of episodic tremor and slip on the Parkfield region of the San Andreas Fault near Cholame, CA. We note the observed occurrence rates of both the tremors and co-located families of low-frequency earthquakes are not steady but instead exhibit quasiperiodic bursts of increased activity.We show that these periods of elevated seismic activity correlate with statistically significant stacked strain signals consisting of 44 slow-slip events. Modeled individual slow-slip events and their total summed moment, which are constrained by seismic signals and stacked strain, respectively, indicate that the individual moment magnitudes of these events range from $M_w$ 4.6–5.2.We find that the measured geodetic signal likely precedes the seismic signal by several hours, consistent with the aseismic slip preceding and driving the observed seismic tremor activity.We confirm that strike-slip faults, in addition to subduction zones, are capable of producing episodic tremor and slip.

58 GEOSCIENCES↗
DOE OSTI2014

Interstellar protons in the TeV γ-ray SNR HESS J1731-347: Possible evidence for the coexistence of hadronic and leptonic γ-rays

HESS J1731-347 (G353.6-0.7) is one of the TeV γ-ray supernova remnants (SNRs) that shows the shell-like morphology. We have made a new analysis of the interstellar protons toward the SNR by using both the {sup 12}CO(J = 1-0) and H I data sets. The results indicate that the TeV γ-ray shell shows significant spatial correlation with the interstellar protons at a velocity range from –90 km s{sup –1} to –75 km s{sup –1}. The total mass of the interstellar medium (ISM) protons is estimated to be 6.4 × 10{sup 4} M {sub ☉}, 25% of which is atomic gas, and the distance corresponding to the velocity range is ∼5.2 kpc, a factor of 2 larger than the previous figure, 3 kpc. We have identified the cold H I gas observed as self-absorption which shows significant correspondence with the northeastern γ-ray peak. While the good correspondence between the ISM protons and TeV γ-rays in the north of the SNR lends support to the hadronic scenario for the TeV γ-rays, the southern part of the shell shows a break in the correspondence; in particular, the southwestern rim of the SNR shell shows a significant decrease of the interstellar protons by a factor of two. We argue that this discrepancy can be explained due to leptonic γ-rays because this region coincides well with the bright shell that emits non-thermal radio continuum emission and non-thermal X-rays, suggesting that the γ-rays of HESS J1713-347 consist of both the hadronic and leptonic components. The leptonic contribution corresponds to ∼20% of the total γ-rays.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2014

Near-infrared structure of fast and slow-rotating disk galaxies

We investigate the stellar disk structure of six nearby edge-on spiral galaxies using high-resolution JHK {sub s}-band images and three-dimensional radiative transfer models. To explore how mass and environment shape spiral disks, we selected galaxies with rotational velocities between 69 km s{sup –1} 150 km s{sup –1}) galaxies, only NGC 4013 has the super-thin+thin+thick nested disk structure seen in NGC 891 and the Milky Way, albeit with decreased oblateness, while NGC 1055, a disturbed massive spiral galaxy, contains disks with h{sub z} ≲ 200 pc. NGC 4565, another fast-rotator, contains a prominent ring at a radius ∼5 kpc but no super-thin disk. Despite these differences, all fast-rotating galaxies in our sample have inner truncations in at least one of their disks. These truncations lead to Freeman Type II profiles when projected face-on. Slow-rotating galaxies are less complex, lacking inner disk truncations and requiring fewer disk components to reproduce their light distributions. Super-thin disk components in undisturbed disks contribute ∼25% of the total K {sub s}-band light, up to that of the thin-disk contribution. The presence of super-thin disks correlates with infrared flux ratios; galaxies with super-thin disks have f{sub K{sub s}}/f{sub 60} {sub μm}≤0.12 for integrated light, consistent with super-thin disks being regions of ongoing star-formation. Attenuation-corrected vertical color gradients in (J – K {sub s}) correlate with the observed disk structure and are consistent with population gradients with young-to-intermediate ages closer to the mid-plane, indicating that disk heating—or cooling—is a ubiquitous phenomenon.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2015

X-ray emission in non-AGN galaxies at z ≃ 1

Using data from the DEEP2 galaxy redshift survey and the All Wavelength Extended Groth Strip International Survey we obtain stacked X-ray maps of galaxies at 0.7⩽z⩽1.0 as a function of stellar mass. We compute the total X-ray counts of these galaxies and show that in the soft band (0.5–2 kev) there exists a significant correlation between galaxy X-ray counts and stellar mass at these redshifts. The best-fit relation between X-ray counts and stellar mass can be characterized by a power law with a slope of 0.58 ± 0.1. We do not find any correlation between stellar mass and X-ray luminosities in the hard (2–7 kev) and ultra-hard (4–7 kev) bands. The derived hardness ratios of our galaxies suggest that the X-ray emission is degenerate between two spectral models, namely point-like power-law emission and extended plasma emission in the interstellar medium. This is similar to what has been observed in low redshift galaxies. Using a simple spectral model where half of the emission comes from power-law sources and the other half from the extended hot halo we derive the X-ray luminosities of our galaxies. The soft X-ray luminosities of our galaxies lie in the range 10{sup 39}–8×10{sup 40} erg s{sup −1}. Dividing our galaxy sample by the criteria U−B>1, we find no evidence that our results for X-ray scaling relations depend on optical color.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2015

The Spitzer survey of stellar structure in galaxies (S{sup 4}G): Precise STELLAR mass distributions from automated dust correction at 3.6 μm

The mid-infrared is an optimal window to trace stellar mass in nearby galaxies and the 3.6 μm IRAC band has been exploited to this effect, but such mass estimates can be biased by dust emission. We present our pipeline to reveal the old stellar flux at 3.6 μm and obtain stellar mass maps for more than 1600 galaxies available from the Spitzer Survey of Stellar Structure in Galaxies (S{sup 4}G). This survey consists of images in two infrared bands (3.6 and 4.5 μm), and we use the Independent Component Analysis (ICA) method presented in Meidt et al. to separate the dominant light from old stars and the dust emission that can significantly contribute to the observed 3.6 μm flux. We exclude from our ICA analysis galaxies with low signal-to-noise ratio (S/N<10) and those with original [3.6]–[4.5] colors compatible with an old stellar population, indicative of little dust emission (mostly early Hubble types, which can directly provide good mass maps). For the remaining 1251 galaxies to which ICA was successfully applied, we find that as much as 10%–30% of the total light at 3.6 μm typically originates from dust, and locally it can reach even higher values. This contamination fraction shows a correlation with specific star formation rates, confirming that the dust emission that we detect is related to star formation. Additionally, we have used our large sample of mass estimates to calibrate a relationship of effective mass-to-light ratio (M/L) as a function of observed [3.6]–[4.5] color: log(M/L) = −0.339(±0.057) × ([3.6]−[4.5])−0.336(±0.002). Our final pipeline products have been made public through IRSA, providing the astronomical community with an unprecedentedly large set of stellar mass maps ready to use for scientific applications.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2017

Energy exchange dynamics across L–H transitions in NSTX

Here, we studied the energy exchange dynamics across the low-to-high-confinement (L–H) transition in NSTX discharges using the gas-puff imaging (GPI) diagnostic. The investigation focused on the energy exchange between flows and turbulence to help clarify the mechanism of the L–H transition. We applied this study to three types of heating schemes, including a total of 17 shots from the NSTX 2010 campaign run. Results show that the edge fluctuation characteristics (fluctuation levels, radial and poloidal correlation lengths) measured using GPI do not vary just prior to the H-mode transition, but change after the transition. Using a velocimetry approach (orthogonal-dynamics programming), velocity fields of a $24\times 30$ cm GPI view during the L–H transition were obtained with good spatial (~1 cm) and temporal (~2.5 μs) resolutions. Analysis using these velocity fields shows that the production term is systematically negative just prior to the L–H transition, indicating a transfer from mean flows to turbulence, which is inconsistent with the predator–prey paradigm. Moreover, the inferred absolute value of the production term is two orders of magnitude too small to explain the observed rapid L–H transition. These discrepancies are further reinforced by consideration of the ratio between the kinetic energy in the mean flow to the thermal free energy, which is estimated to be much less than 1, suggesting again that the turbulence depletion mechanism may not play an important role in the transition to the H-mode. Although the Reynolds work therefore appears to be too small to directly deplete the turbulent free energy reservoir, order-of-magnitude analysis shows that the Reynolds stress may still make a non-negligible contribution to the observed poloidal flows.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2024

Coupled model intercomparison project phase 6 (CMIP6) high resolution model intercomparison project (HighResMIP) bias in extreme rainfall drives underestimation of amazonian precipitation

Abstract Extreme rainfall events drive the amount and spatial distribution of rainfall in the Amazon and are a key driver of forest dynamics across the basin. This study investigates how the 3-hourly predictions in the High Resolution Model Intercomparison Project (HighResMIP, a component of the recent Coupled Model Intercomparison Project, CMIP6) represent extreme rainfall events at annual, seasonal, and sub-daily time scales. TRMM 3B42 (Tropical Rainfall Measuring Mission) 3 h data were used as observations. Our results showed that eleven out of seventeen HighResMIP models showed the observed association between rainfall and number of extreme events at the annual and seasonal scales. Two models captured the spatial pattern of number of extreme events at the seasonal and annual scales better (higher correlation) than the other models. None of the models captured the sub-daily timing of extreme rainfall, though some reproduced daily totals. Our results suggest that higher model resolution is a crucial factor for capturing extreme rainfall events in the Amazon, but it might not be the sole factor. Improving the representation of Amazon extreme rainfall events in HighResMIP models can help reduce model rainfall biases and uncertainties and enable more reliable assessments of the water cycle and forest dynamics in the Amazon.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2022

First-principles study of L -shell iron and chromium opacity at stellar interior temperatures

Recently developed free-energy density functional theory (DFT)-based methodology for optical property calculations of warm dense matter has been applied for studying L-shell opacity of iron and chromium at T = 182 eV. We use Mermin–Kohn–Sham density functional theory with a ground-state and a fully temperature-dependent generalized gradient approximation exchange-correlation (XC) functionals. It is demonstrated that the role of XC at such a high-T is negligible due to the total free-energy of interacting system being dominated by the noninteracting free-energy term in agreement with estimations for the homogeneous electron gas. Furthermore, our DFT predictions are compared to the radiative emissivity and opacity of dense plasmas model, to the real-space Greens function method, and to experimental measurements. Good agreement is found between all three theoretical methods, and in the bound–continuum region for Cr when compared to the experiment, while the discrepancy between direct DFT calculations and the experiment for Fe remains essentially the same as for plasma-physics models.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2017

Energy Exchange Dynamics across L-H Transitions in NSTX

We studied the energy exchange dynamics across the low-to-high-confinement (L-H) in NSTX discharges using the gas-puff imaging (GPI) diagnotic. The investigation focused on the energy exchange between flows and turbulence, to help clarify the mechanism of the L-H transition. We apply this study to three type of heating schemes, including a total of 17 shots from the NSTX 2010 campaign run. Results show that the edge fluctuation characteristics (fluctuation levels, radial and poloidal correlation lengths) measured using GPI do not vary just prior to the H-mode transition, but change after the transition. Using a velocimetry approach (orthogonal-programming decomposition), velocity fields of a 24 $\times$ 30 cm GPI view during the L-H transition were obtained with good spatial ($\sim$1 cm) and temporal ($\sim$2.5 $\mu$s) resolutions. Analysis using these velocity fields shows that the production term is systematically negative just prior to the L-H transition indicating transfer from mean flows to turbulence, which is inconsistent with the predator-prey paradigm. Moreover, using the inferred absolute value of the production term, an estimate of the L-H transition duration is found to be 25 ms, which is much larger than the measured duration. These discrepancies are further reinforced by consideration of the ratio between the kinetic energy in the mean flow to the thermal free energy, which is estimated to be much less than 1, suggesting again that turbulence depletion mechanism may not be playing an important role in the transition to the H-mode. Although the Reynolds work is too small to directly deplete the turbulent free energy reservoir, order-of-magnitude analysis shows that the Reynolds stress may still make a non-negligible contribution to the observed poloidal flows.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2014

An Integrated Study to Analyze Soil Microbial Community Structure and Metabolic Potential in Two Forest Types

Soil microbial metabolic potential and ecosystem function have received little attention owing to difficulties in methodology. In this study, we selected natural mature forest and natural secondary forest and analyzed the soil microbial community and metabolic potential combing the high-throughput sequencing and GeoChip technologies. Phylogenetic analysis based on 16S rRNA sequencing showed that one known archaeal phylum and 15 known bacterial phyla as well as unclassified phylotypes were presented in these forest soils, and Acidobacteria, Protecobacteria, and Actinobacteria were three of most abundant phyla. The detected microbial functional gene groups were related to different biogeochemical processes, including carbon degradation, carbon fixation, methane metabolism, nitrogen cycling, phosphorus utilization, sulfur cycling, etc. The Shannon index for detected functional gene probes was significantly higher (P<0.05) at natural secondary forest site. The regression analysis showed that a strong positive (P<0.05) correlation was existed between the soil microbial functional gene diversity and phylogenetic diversity. Mantel test showed that soil oxidizable organic carbon, soil total nitrogen and cellulose, glucanase, and amylase activities were significantly linked (P<0.05) to the relative abundance of corresponded functional gene groups. Variance partitioning analysis showed that a total of 81.58% of the variation in community structure was explained by soil chemical factors, soil temperature, and plant diversity. Therefore, the positive link of soil microbial structure and composition to functional activity related to ecosystem functioning was existed, and the natural secondary forest soil may occur the high microbial metabolic potential. Although the results can’t directly reflect the actual microbial populations and functional activities, this study provides insight into the potential activity of the microbial community and associated feedback responses of the terrestrial ecosystem to environmental changes.

54 ENVIRONMENTAL SCIENCES↗