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At least 1,513 records · Page 84
All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

CRISPR-COPIES: Web Tool

CRISPR/Cas system has emerged as a powerful genome-editing tool for metabolic engineering and human gene therapy. However, the conundrum of where to integrate heterologous genes on the chromosome using the CRISPR/Cas system remains an open question. Selecting a site for gene integration requires incorporation of complex criteria such as factors involved in CRISPR/Cas-mediated integration, genetic stability, and gene expression and therefore, usually requires strenuous characterization of sites on particular or different chromosomal locations. To address these issues, we developed CRISPR-COPIES, a COmputational Pipeline for the Identification of CRISPR/Cas-facilitated intEgration Sites. The tool applies ScaNN, a state-of-the-art model on the embedding-based nearest neighbor search for fast and accurate off-target search and can identify genome-wide intergenic sites for most bacterial and fungal genomes within minutes. This submission contains the code we developed to create a user-friendly web interface for CRISPR-COPIES (https://biofoundry.web.illinois.edu/copies/). We anticipate CRISPR-COPIES will serve as a useful tool for targeted DNA integration and aid in the characterization of synthetic biology toolkits, rapid strain construction to produce valuable biochemicals, and human gene and cell therapy.

Bioinformatics↗
DOE OSTI2018

CERF – A Geospatial Model for Assessing Future Energy Production Technology Expansion Feasibility

Electricity system expansion planning faces significant challenges not only because of commonly modeled uncertainties such as future demand growth, fuel prices, and technology cost and performance, but also because of challenges such as climate change and carbon policies that can affect supply and demand. Changes in energy technology, natural resource availability, and population migration and growth will affect the feasibility of power plant siting, potentially hindering the achievement of policy goals. New modeling approaches are needed that couple future projections of energy system expansion with “on the ground” feasibility modeling—where both are informed by a consistent set of assumptions about future climate trends, resource availability, climate policy, socioeconomics, and environmental regulations. This research describes the development and evaluation of the Capacity Expansion Regional Feasibility (CERF) model, a geospatial tool for scenario analysis of electricity system expansions.

grid expansion, water-energy nexus, power plant si↗
NASA NTRS1974

Venus - Mass, gravity field, atmosphere, and ionosphere as measured by the Mariner 10 dual-frequency radio system

The unique properties of the Mariner 10 radio system, and the preliminary scientific results obtained from the analysis of the radio signals are described. In the normal two-way communication mode, a command- and range-modulated 2115-MHz signal is transmitted to the spacecraft for reception on its omnidirectional antenna. As implemented for Mariner 10, the dual-frequency system has proven fully capable of performing interplanetary columnar electron content measurements while achieving the prime goals of the celestial mechanics and radio science team. The determination of the mass and gravitational potential of Venus is one of the major objectives of the radio science experiments. Information on Venus's atmosphere was deduced from analysis of the radio signals during occultation. Open-loop receiver differential Doppler data were used to measure the nightside and dayside ionospheres of Venus.

Howard, H. T.↗
NASA NTRS1987

USSR Space Life Sciences Digest, issue 9

This is the ninth issue of NASA's USSR Space Lifes Sciences Digest. It contains abstracts of 46 papers recently published in Russian language periodicals and bound collections and of a new Soviet monograph. Selected abstracts are illustrated with figures and tables from the original. Additional features include reviews of a Russian book on biological rhythms and a description of the papers presented at a conference on space biology and medicine. A special feature describes two paradigms frequently cited in Soviet space life sciences literature. Information about English translations of Soviet materials available to readers is provided. The abstracts included in this issue have been identified as relevant to 28 areas of aerospace medicine and space biology. These areas are: adaptation, biological rhythms, body fluids, botany, cardiovascular and respiratory systems, developmental biology, endocrinology, enzymology, equipment and instrumentation, gastrointestinal system, genetics, habitability and environment effects, hematology, human performance, immunology, life support systems, mathematical modeling, metabolism, microbiology, morphology and cytology, musculoskeletal system, nutrition, neurophysiology, operational medicine, perception, personnel selection, psychology, radiobiology, and space biology and medicine.

Hooke, Lydia Razran↗
NASA NTRS1993

The Space and Earth Science Data Compression Workshop

This document is the proceedings from a Space and Earth Science Data Compression Workshop, which was held on March 27, 1992, at the Snowbird Conference Center in Snowbird, Utah. This workshop was held in conjunction with the 1992 Data Compression Conference (DCC '92), which was held at the same location, March 24-26, 1992. The workshop explored opportunities for data compression to enhance the collection and analysis of space and Earth science data. The workshop consisted of eleven papers presented in four sessions. These papers describe research that is integrated into, or has the potential of being integrated into, a particular space and/or Earth science data information system. Presenters were encouraged to take into account the scientists's data requirements, and the constraints imposed by the data collection, transmission, distribution, and archival system.

Tilton, James C.↗
NASA NTRS1990

Information Security and Integrity Systems

Viewgraphs from the Information Security and Integrity Systems seminar held at the University of Houston-Clear Lake on May 15-16, 1990 are presented. A tutorial on computer security is presented. The goals of this tutorial are the following: to review security requirements imposed by government and by common sense; to examine risk analysis methods to help keep sight of forest while in trees; to discuss the current hot topic of viruses (which will stay hot); to examine network security, now and in the next year to 30 years; to give a brief overview of encryption; to review protection methods in operating systems; to review database security problems; to review the Trusted Computer System Evaluation Criteria (Orange Book); to comment on formal verification methods; to consider new approaches (like intrusion detection and biometrics); to review the old, low tech, and still good solutions; and to give pointers to the literature and to where to get help. Other topics covered include security in software applications and development; risk management; trust: formal methods and associated techniques; secure distributed operating system and verification; trusted Ada; a conceptual model for supporting a B3+ dynamic multilevel security and integrity in the Ada runtime environment; and information intelligence sciences.

Source record↗
NASA NTRS1994

The 1994 Space and Earth Science Data Compression Workshop

This document is the proceedings from the fourth annual 'Space and Earth Science Data Compression Workshop,' which was held on April 2, 1994, at the University of Utah in Salt Lake City, Utah. This workshop was held in cooperation with the 1994 Data Compression Conference, which was held at Snowbird, Utah, March 29-31 1994. The Workshop explored opportunities for data compression to enhance the collection and analysis of space and Earth science data. It consisted of 13 papers presented in 4 sessions. The papers focus on data compression research that is integrated into, or has the potential to be integrated into, a particular space and/or Earth science data information system. Presenters were encouraged to take into account the scientist's data requirements, and the constraints imposed by the data collection, transmission, distribution, and archival system.

Tilton, James C.↗
NASA NTRS1995

The Telestation: A product for distributed data gathering and operations

Advances in computer, navigation and wireless telecommunication technologies are enabling better electronic devices at reduced costs. Individually, these devices have been applied to increase the performance of many common systems, such as desktop computers, surveying equipment, and modems. However, we believe the convergence of these three technologies will create a product that simultaneously lowers life cycle costs while increasing operational effectiveness. Space Systems/Loral (SS/L) is developing portions of this product, known as the Telestation, for both terrestrial and orbital applications. The Telestation consists of an advanced microprocessor for command and data handling; a GPS receiver for position, time, and attitude information; and a Globalstar Transceiver for two-way digital communications. The Telestation provides the user with real-time command and control of globally distributed hardware elements. This capability can be applied terrestrially for gathering information (e.g., science, environmental, etc.) in remote or inhospitable locations, or where logistical support is inadequate. An in-orbit version can be used for spacecraft or payload operations, allowing principal investigators instant access to their payloads during all phases of a mission. This paper describes some cost effectiveness metrics of the Telestation, its development status, and its utility in both terrestrial and orbital applications.

Cleave, Robert R.↗
NASA NTRS1996

A mission operations architecture for the 21st century

An operations architecture is proposed for low cost missions beyond the year 2000. The architecture consists of three elements: a service based architecture; a demand access automata; and distributed science hubs. The service based architecture is based on a set of standard multimission services that are defined, packaged and formalized by the deep space network and the advanced multi-mission operations system. The demand access automata is a suite of technologies which reduces the need to be in contact with the spacecraft, and thus reduces operating costs. The beacon signaling, the virtual emergency room, and the high efficiency tracking automata technologies are described. The distributed science hubs provide information system capabilities to the small science oriented flight teams: individual access to all traditional mission functions and services; multimedia intra-team communications, and automated direct transparent communications between the scientists and the instrument.

Tai, W.↗
NASA NTRS1996

Combined Industry, Space and Earth Science Data Compression Workshop

The sixth annual Space and Earth Science Data Compression Workshop and the third annual Data Compression Industry Workshop were held as a single combined workshop. The workshop was held April 4, 1996 in Snowbird, Utah in conjunction with the 1996 IEEE Data Compression Conference, which was held at the same location March 31 - April 3, 1996. The Space and Earth Science Data Compression sessions seek to explore opportunities for data compression to enhance the collection, analysis, and retrieval of space and earth science data. Of particular interest is data compression research that is integrated into, or has the potential to be integrated into, a particular space or earth science data information system. Preference is given to data compression research that takes into account the scien- tist's data requirements, and the constraints imposed by the data collection, transmission, distribution and archival systems.

Kiely, Aaron B.↗
NASA NTRS1996

Development of Collaborative Research Initiatives to Advance the Aerospace Sciences-via the Communications, Electronics, Information Systems Focus Group

The primary goal of the Adaptive Vision Laboratory Research project was to develop advanced computer vision systems for automatic target recognition. The approach used in this effort combined several machine learning paradigms including evolutionary learning algorithms, neural networks, and adaptive clustering techniques to develop the E-MOR.PH system. This system is capable of generating pattern recognition systems to solve a wide variety of complex recognition tasks. A series of simulation experiments were conducted using E-MORPH to solve problems in OCR, military target recognition, industrial inspection, and medical image analysis. The bulk of the funds provided through this grant were used to purchase computer hardware and software to support these computationally intensive simulations. The payoff from this effort is the reduced need for human involvement in the design and implementation of recognition systems. We have shown that the techniques used in E-MORPH are generic and readily transition to other problem domains. Specifically, E-MORPH is multi-phase evolutionary leaming system that evolves cooperative sets of features detectors and combines their response using an adaptive classifier to form a complete pattern recognition system. The system can operate on binary or grayscale images. In our most recent experiments, we used multi-resolution images that are formed by applying a Gabor wavelet transform to a set of grayscale input images. To begin the leaming process, candidate chips are extracted from the multi-resolution images to form a training set and a test set. A population of detector sets is randomly initialized to start the evolutionary process. Using a combination of evolutionary programming and genetic algorithms, the feature detectors are enhanced to solve a recognition problem. The design of E-MORPH and recognition results for a complex problem in medical image analysis are described at the end of this report. The specific task involves the identification of vertebrae in x-ray images of human spinal columns. This problem is extremely challenging because the individual vertebra exhibit variation in shape, scale, orientation, and contrast. E-MORPH generated several accurate recognition systems to solve this task. This dual use of this ATR technology clearly demonstrates the flexibility and power of our approach.

Knasel, T. Michael↗
NASA NTRS2000

Testing the BUNDLE Experiment for Microgravity Disturbance Characterization

A primary objective of the International Space Station is to provide a premier facility for microgravity research. There are many disturbances on board the Station that can disturb the quality of the microgravity environment, including disturbances caused by both system and payload hardware. Disturbances within experiments themselves can also affect the quality of their own environment. The primary functions of the Bridgman Unidirectional Dendrites in Liquids Experiment (BUNDLE) are to melt, directionally solidify, and perform in-situ quench of metallic alloys, providing data to aid in understanding the effects of the microgravity environment on the characteristics of these processed metals. Quenching of the samples in the BUNDLE furnace is accomplished by rapidly introducing high pressure helium into the furnace chamber. This type of quench will induce sample vibrations which may be large enough to adversely affect sample quality. The primary objective of the test described in this presentation was to determine the acceleration imparted on the BUNDLE sample during helium quench. From this information, the science community can better assess whether this method of quench will allow them to obtain the data they need. Utilizing breadboard hardware, the sample quench sequence was conducted. Data was collected from accelerometers located on the breadboard hardware and inside the sample cartridge. The test data indicated that the maximum acceleration achieved by the sample was 0.325 g, which is much higher than the desired microgravity (10(exp -6) g) environment. However, this acceleration level occurred over a time period of less than 0.1 second. Because this time period is so short, there should be no detrimental effects to dendrite growth. This result was consistent with the observations of the experiment scientists, who are satisfied with the results of previous dendrite growth in this breadboard unit.

Gattis, Christy↗
NASA NTRS2001

Jupiter: Atmospheric Sounding and Sensing of the Interior (JASSI)

The formation of the giant planets is one of the most fundamental questions in solar system exploration. Understanding the process that led to the creation of Jupiter is essential to understanding the nature of the primordial solar nebula, and the formation of our solar system and others currently being discovered. Data from Galileo combined with HST and Ulysses results validated our basic understanding of Jupiter as a giant planet whose gaseous envelope consists of solar nebula gas enriched in elements heavier than He by in falling icy planetesimals. However, the current Galileo Probe data set does not itself allow firm conclusions about the original planetesimal composition or the process of giant planet formation - we crucially need the O and N abundances that Galileo could not determine. We propose a new and simple concept capable of determining these abundance in Jupiter plus substantial gravity science. Additional information is contained in the original extended abstract.

Bolton, S. J.↗
NASA NTRS2001

Titan Orbiter Aerorover Mission

We propose a combined Titan orbiter and Titan Aerorover mission with an emphasis on both in situ and remote sensing measurements of Titan's surface, atmosphere, ionosphere, and magnetospheric interaction. The biological aspect of the Titan environment will be emphasized by the mission (i.e., search for organic materials which may include simple organics to 'amono' analogues of amino acids and possibly more complex, lightening detection and infrared, ultraviolet, and charged particle interactions with Titan's surface and atmosphere). An international mission is assumed to control costs. NASA will provide the orbiter, launch vehicle, DSN coverage and operations, while international partners will provide the Aerorover and up to 30% of the cost for the scientific instruments through collaborative efforts. To further reduce costs we propose a single PI for orbiter science instruments and a single PI for Aerorover science instruments. This approach will provide single command/data and power interface between spacecraft and orbiter instruments that will have redundant central DPU and power converter for their instruments. A similar approach could be used for the Aerorover. The mission profile will be constructed to minimize conflicts between Aerorover science, orbiter radar science, orbiter radio science, orbiter imaging science, and orbiter fields and particles (FP) science. Additional information is contained in the original extended abstract.

Sittler Jr., E. C.↗
NASA NTRS2001

Issues in Informal Education: Event-Based Science Communication Involving Planetaria and the Internet

For the last several years the Science Directorate at Marshall Space Flight Center has carried out a diverse program of Internet-based science communication. The program includes extended stories about NASA science, a curriculum resource for teachers tied to national education standards, on-line activities for students, and webcasts of real-time events. The focus of sharing real-time science related events has been to involve and excite students and the public about science. Events have involved meteor showers, solar eclipses, natural very low frequency radio emissions, and amateur balloon flights. In some cases broadcasts accommodate active feedback and questions from Internet participants. Panel participation will be used to communicate the problems and lessons learned from these activities over the last three years.

Adams, Mitzi L.↗