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All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

Increased inflammation as well as decreased endoplasmic reticulum stress and translation differentiate pancreatic islets from donors with pre-symptomatic stage 1 type 1 diabetes and non-diabetic donors

Aims/hypothesis Progression to type 1 diabetes is associated with genetic factors, the presence of autoantibodies and a decline in beta cell insulin secretion in response to glucose. Very little is known regarding the molecular changes that occur in human insulin-secreting beta cells prior to the onset of type 1 diabetes. Herein, we applied an unbiased proteomics approach to identify changes in proteins and potential mechanisms of islet dysfunction in islet-autoantibody-positive organ donors with pre-symptomatic stage 1 type 1 diabetes (HbA1c ≤42 mmol/mol [6.0%]). We aimed to identify pathways in islets that are indicative of beta cell dysfunction. Methods Multiple islet sections were collected through laser microdissection of frozen pancreatic tissues from organ donors positive for single or multiple islet autoantibodies (AAb + , n=5), and age (±2 years)- and sex-matched non-diabetic (ND) control donors (n=5) obtained from the Network for Pancreatic Organ donors with Diabetes (nPOD). Islet sections were subjected to MS-based proteomics and analysed with label-free quantification followed by pathway and functional annotations. Results Analyses resulted in ~4500 proteins identified with low false discovery rate (<1%), with 2165 proteins reliably quantified in every islet sample. We observed large inter-donor variations that presented a challenge for statistical analysis of proteome changes between donor groups. We therefore focused on only the donors with stage 1 type 1 diabetes who were positive for multiple autoantibodies (mAAb + , n=3) and genetic risk compared with their matched ND controls (n=3) for the final statistical analysis. Approximately 10% of the proteins (n=202) were significantly different (unadjusted p<0.025, q<0.15) for mAAb + vs ND donor islets. The significant alterations clustered around major functions for upregulation in the immune response and glycolysis, and downregulation in endoplasmic reticulum (ER) stress response as well as protein translation and synthesis. The observed proteome changes were further supported by several independent published datasets, including a proteomics dataset from in vitro proinflammatory cytokine-treated human islets and single-cell RNA-seq datasets from AAb + individuals. Conclusions/interpretation In situ human islet proteome alterations in stage 1 type 1 diabetes centred around several major functional categories, including an expected increase in immune response genes (elevated antigen presentation/HLA), with decreases in protein synthesis and ER stress response, as well as compensatory metabolic response. The dataset serves as a proteomics resource for future studies on beta cell changes during type 1 diabetes progression and pathogenesis. Data availability The LC-MS raw datasets that support the findings of this study have been deposited in the online repository: MassIVE (https://massive.ucsd.edu/ProteoSAFe/static/massive.jsp) with accession no. MSV000090212.

Autoantibody-positive↗
DOE OSTI2022

Spectral evidence of solar neighborhood analogs in CALIFA galaxies

We introduce a novel nonparametric method to find solar neighborhood analogs (SNAs) in extragalactic integral field spectroscopic surveys. The main ansatz is that the physical properties of the solar neighborhood (SN) should be encoded in its optical stellar spectrum. We assume that our best estimate of such a spectrum is the one extracted from the analysis performed by the Code for Stellar properties Heuristic Assignment (CoSHA) from the MaStar stellar library. It follows that finding SNAs in other galaxies consist in matching, in a χ 2 sense, the SN reference spectrum across the optical extent of the observed galaxies. We applied this procedure to a selection of CALIFA galaxies, by requiring a close to face-on projection, relative isolation, and non-active galactic nucleus. We explore how the local and global properties of the SNAs (stellar age, metallicity, dust extinction, mass-to-light ratio, stellar surface mass density, star-formation density, and galactocentric distance) and their corresponding host galaxies (morphological type, total stellar mass, star-formation rate, and effective radius) compared with those of the SN and the Milky Way (MW). We find that SNAs are located preferentially in S(B)a–S(B)c galaxies, in a ring-like structure, which radii seem to scale with the galaxy size. Despite the known sources of systematics and errors, most properties present a considerable agreement with the literature on the SN. We conclude that the solar neighborhood is relatively common in our sample of SNAs. Our results warrant a systematic exploration of correlations among the physical properties of the SNAs and their host galaxies. We reckon that our method should inform current models of the galactic habitable zone in our MW and other galaxies.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2021

Oak Ridge National Laboratory Roane and Anderson Counties, Tennessee Historic Architectural Resource Survey June 2018

From February through September 2017, Cultural Resource Analysts, Inc. personnel completed a historic architectural resource survey of the Oak Ridge National Laboratory in Roane and Anderson Counties, Tennessee. The survey was completed at the request of UT-Battelle, LLC, the private not-for-profit company that manages and operates Oak Ridge National Laboratory for the U.S. Department of Energy. The purpose of the survey was to assist the Department of Energy in complying with the National Historic Preservation Act of 1966, as amended, by providing updated recommendations regarding the National Register of Historic Places eligibility of historic architectural resources at Oak Ridge National Laboratory. The survey included the entirety of Oak Ridge National Laboratory’s main campus that occupies approximately 1,100 acres in Bethel and Melton Valleys and on Chestnut Ridge. Based on Facilities Information Management System data and guidance provided by UT-Battelle, LLC, preservation and modernization staff, the survey team developed a list of resources for inclusion in the survey. The list included all substantial buildings constructed in or before 1980 in order to provide sufficient coverage of buildings that will turn 50 years of age within the next decade. Only those buildings with recorded area over 100 sq ft were included in the survey. Additionally, the UT-Battelle team identified 15 buildings for more intensive survey because such buildings are central to Oak Ridge National Laboratory’s future mission needs and are expected to undergo renovations in the foreseeable future. These building were subject to both exterior and interior documentation with particular focus on identifying character-defining features, and thus were subject to more detailed analysis than the other resources included in the survey. The findings of this historic resource survey build on the conclusions of the 1994 survey by DuVall & Associates, Inc., as well as the survey updates completed by Thomason and Associates in 2004 and 2015 in support of the Oak Ridge National Laboratory Historic Preservation Plan. Within 12 distinct areas of Oak Ridge National Laboratory, as well as outlying areas, the survey recorded 195 buildings and structures constructed in or before 1980. In consideration of the role and significance of each area and each individual resource within the context of Oak Ridge National Laboratory, and in light of their current integrity and condition, each recorded resource was evaluated to determine if it is eligible for listing in the National Register of Historic Places.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗
NASA NTRS1973

Evaluation of ERTS-1 imagery for mapping Quaternary deposits and landforms in the Great Plains and Midwest

The author has identified the following significant results. The main landform associations and larger landforms are readily identifiable on the better images and commonly the gross associations of surficial Quaternary deposits also can be differentiated, primarily by information on landforms and soils. Maps showing the Quaternary geologic-terrain units that can be differentiated from the ERTS-1 images are being prepared for 20-odd potential study areas in Illinois, Iowa, Missouri, Kansas, Nebraska, and South Dakota. Among the more distinct features are the major moraines and outwash channels of the last (Wisconsin) glaciation. Analysis of dissection/drainage patterns from the synoptic imagery is proving useful for detecting anomalies that may be caused by stream diversions and moraines of pre-Wisconsin glaciations, by variable loess deposition, by tectonism, and other factors. Numerous abandoned river valleys have been mapped. Trend-lines of several known pre-Wisconsin moraine systems have been identified in Iowa, Nebraska, and Kansas, and also several similar trend-lines, that may indicate previously unknown moraine systems of middle and possibly early Pleistocene age, have been found in Iowa and Missouri. The area inundated by a major flood in southwestern Iowa also has been delineated from ERTS-1 imagery.

Morrison, R. B.↗
NASA NTRS2003

Chandra X-Ray Observations of the Spiral Galaxy M81

A Chandra X-Ray Observatory ACIS-S imaging observation is used to study the population of X-ray sources in the nearby Sab galaxy M81 (NGC 3031). A total of 177 sources are detected with 124 located within the D(sub 25) isophote to a limiting X-ray luminosity of approx. 3 x 10(exp 36) ergs/s. Source positions, count rates, luminosities in the 0.3-8.0 keV band, limiting optical magnitudes, and potential counterpart identifications are tabulated. Spectral and timing analysis of the 36 brightest sources are reported including the low luminosity active galactic nucleus, SN 1993J, and the Einstein-discovered ultraluminous X-ray source X6. The nucleus accounts for approx. 86%, or 5 x 10(exp 40) ergs/s, of the total X-ray emission from M81. Its spectrum is well fitted by an absorbed power law with photon index 1.98 +/- 0.08, consistent with previous observations (average index 1.9). SN 1993J has softened and faded since its discovery. At an age of 2594 days, SN 1993J displayed a complex thermal spectrum from a reverse shock rich in Fe L and highly ionized Mg, Si, and S but lacking O. A hard X-ray component, emitted by a forward shock, is also present. X6 is spatially coincident with a stellar object with optical brightness and colors consistent with an O9-B1 main-sequence star. It is also coincident with a weak radio source with a flux density of approx. 95 microJy at lambda = 3.6 cm. The continuum-dominated X-ray spectrum of X6 is most closely reproduced by a blackbody disk model suggesting the X-ray source is an approx. 18 solar mass object accreting at nearly its Eddington limit.

Swartz, Douglas A.↗
NASA NTRS2020

Climate and Anthropogenic Controls on Blue Carbon Sequestration in Hudson River Tidal Marsh, Piermont, New York

Tidal marshes globally are experiencing erosion with sea level rise. In order to adaptively plan for essential marsh preservation, we recognize the importance of the investigation of marsh archives for the perspective they provide toward resilience. Our objective in this study is to examine the relationship of tidal marsh carbon sequestration with both climate change and human impact throughout past centuries and millennia. A Hudson River marsh sediment core spanning the last 2000 years is analyzed for bulk loss on ignition (LOI), bulk density, sedimentation rate, carbon (C) and mineral flux, and x-ray fluorescence (XRF) analysis including lead, copper, titanium and potassium. We compare this record to previously established pollen and spore stratigraphy from the same site, along with an extensive macrofossil based AMS 14C chronology based upon both cores. Carbon accumulation generally follows sediment accumulation rates, which were higher than 200 g C m−2 yr−1 prior to 1500 years ago. Declines in carbon storage rate during the Medieval Warm Period (MWP) are linked to drought, fire, and charcoal, while lesser declines during the Little Ice Age (LIA) are linked to cooling and a shorter growing season. Subsequent human impact with marsh haying practices also led to carbon accumulation rate decline to 100 g C m−2 yr−1. Increases in C sequestration rates in recent decades may be attributable to nitrogen pollution of the estuary, invasive plants, and/or increased flooding, but the lack of mineral sediment threatens their stability. Ecosystem function is declining with the loss of foundational species, and the crisis is deepening for preservation of this habitat. We strongly recommend strategies for minimizing marsh loss.

Climate controls↗
NASA NTRS2013

Evidence for Perchlorates and the Origin of Chlorinated Hydrocarbons Detected by SAM at the Rocknest Aeolian Deposit in Gale Crater

Four individual sample portions from a single scoop of the Rocknest aeolian deposit were sieved ( 150 m) and delivered to the Sample Analysis at Mars (SAM) instrument by the Mars Science Laboratory rover's sample acquisition system. The samples were analyzed separately by the SAM pyrolysis evolved gas and gas chromatography mass spectrometry analysis modes. Several chlorinated hydrocarbons including chloromethane, dichloromethane, trichloromethane, a chloromethylpropene, and chlorobenzene were identified by SAM above background levels with abundances of 0.01 to 2.3 nanomole.The evolution of the chloromethanes observed during pyrolysis is coincident with the increase in O2 released from the Rocknest sample and the decomposition of a product of N-methyl-N- (tert-butyldimethylsilyl)trifluoroacetamide (MTBSTFA), a chemical that leaked from a derivatization cup inside SAM.The best candidate for the oxychloride phase in Rocknest is a hydrated calcium perchlorate (Ca(ClO4)2nH2O), based on the temperature release of O2 that correlates with the release of the chlorinated species measured by SAM, although other chlorine bearing phases are being considered. Laboratory pyrolysis experiments suggest that reaction of martian chlorine with organic carbon from MTBSTFA can explain the presence of the chloromethanes and a chloromethylpropene also detected by SAM.However, we cannot exclude the possibility that traces of organic carbon of either martian or exogenous origin contributed to some of the chloromethanes measured by SAM. Although the alteration history and exposure age of the Rocknest deposit is unknown, it is possible that oxidative degradation of complex organic matter by ionizing radiation or other chemical processes in Rocknest has occurred.

Mars Rover↗
DOE OSTI2026

Adapting Nuclear Forensics from Light Water to Molten Salt Reactors: A Survey of Emerging Needs

Rising interest in molten salt reactors for commercial power production presents an opportunity to evaluate the techniques used to characterize materials of nuclear forensic interest. Since extensive research has been performed to identify and develop signatures of light water reactor (LWR) materials (e.g. uranium ore concentrates and uranium dioxide fuel pellets), we use this as a basis to explore possibilities for molten salt signature development. Through this comparative method, nuclear forensic signatures used today to identify the provenance of nuclear materials found out of regulatory control are adapted to molten salt reactor (MSR) fuel cycle materials. Radiological, elemental composition, isotopic composition, and model age signatures will likely not need large adaptations before being applied to MSR materials but may need to expand to be applicable to both thorium- and uranium-fueled systems. The liquid nature of molten salt fuel may erase signatures related to production and irradiation history that are informative for typical LWR materials. However, it may also lead to opportunities for new signatures, such as cooling rate–controlled morphology. Targets identified for further research include radiological attributes of fuel salts; elemental, chemical, and isotopic analysis of salts with differing production routes; morphological effects of various thermodynamic environments; and relevant fuel cycle radiochronometers. Additionally, the comparative nature of the proposed signatures implies a need for MSR-relevant databases and the production of salt standard reference materials.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗
DOE OSTI2026

A Community Guide to Regulatory Barriers Affecting Microgrids (Reports 1-3)

In response to growing risks of power outages from extreme weather and aging infrastructure, communities are increasingly exploring the potential of microgrids to provide reliable energy access. Microgrids offer promising solutions to meet this challenge but face a complex landscape of non-technical barriers, particularly regulations concerning the provision and distribution of energy. Most existing legal and regulatory frameworks were designed for a centralized, one-way power system, and are often poorly suited to handle systems that independently balance distributed energy resources with local load. This three-part report series provides a strategic analysis of existing regulatory and legal factors affecting microgrid deployments to help non-technical community leaders and decision-makers better understand the feasibility of a microgrid in their community. -Report No. 1: Foundational Issues Facing Microgrids details the universal policy barriers all microgrids face, including utility interconnection processes, rate structures, and local permitting. -Report No. 2: Single Property Microgrids outlines how direct asset ownership and operating behind-the-meter can bypass some regulatory oversight, using the Blue Lake Rancheria microgrid as a case study. -Report No. 3: Multi-Property Microgrids tackles the complex challenges of crossing public rights-of-way and navigating utility franchise rights, highlighting the Coventry microgrid project. The series equips decision-makers with phased frameworks to navigate financial and regulatory complexities, engage effectively with local utilities and Authorities Having Jurisdiction (AHJs), and structure successful microgrid projects.

24 POWER TRANSMISSION AND DISTRIBUTION↗
DOE OSTI2024

Effect of Environmental and Socioeconomic Factors on Increased Early Childhood Blood Lead Levels: A Case Study in Chicago

This study analyzes the prevalence of elevated blood lead levels (BLLs) in children across Chicagoland zip codes from 2019 to 2021, linking them to socioeconomic, environmental, and racial factors. Wilcoxon tests and generalized additive model (GAM) regressions identified economic hardship, reflected in per capita income and unemployment rates, as a significant contributor to increased lead poisoning (LP) rates. Additionally, LP rates correlate with the average age of buildings, particularly post the 1978 lead paint ban, illustrating policy impacts on health outcomes. The study further explores the novel area of land surface temperature (LST) effects on LP, finding that higher nighttime LST, indicative of urban heat island effects, correlates with increased LP. This finding gains additional significance in the context of anthropogenic climate change. When these factors are combined with the ongoing expansion of urban territories, a significant risk exists of escalating LP rates on a global scale. Racial disparity analysis revealed that Black and Hispanic/Latino populations face higher LP rates, primarily due to unemployment and older housing. The study underscores the necessity for targeted public health strategies to address these disparities, emphasizing the need for interventions that cater to the unique challenges of these at-risk communities.

Lee, Jangho (ORCID:0000000289421092)↗
NASA NTRS1988

Preliminary report on radiocarbon dating of cryptoendolithic microorganisms

The existence of microbial communities living inside desert rocks has been reported by FRIEDMANN et al. (1967, 1976), first in rocks collected from the hot and dry Negev desert and later in rocks in the frigid Ross Desert of Southern Victoria Land, Antarctica. The extremely inhospitable climatic conditions in both places has led to the suggestion that these organisms have very low rates of metabolism and may, in addition, be very old (FRIEDMANN 1982). Our preliminary measurements showed a 14C deficiency indicating a carbon age in the order of magnitude of 10(3) years.

NASA Program Exobiology↗
NASA NTRS2010

Discovery of X-Ray-Emitting O-Ne-Mg-Rich Ejecta in the Galactic Supernova Remnant Puppis A

We report on the discovery of X-ray-emitting O-Ne-Mg-rich ejecta in the middle-aged Galactic O-rich supernova remnant Puppis A with Chandra and XMM-Newton. We use line ratios to identify a low-ionization filament running parallel to the northeastern edge of the remnant that requires super-solar abundances, particularly for O, Ne, and Mg, which we interpret to be from O-Ne-Mg-rich ejecta. Abundance ratios of Ne/O, Mg/O, and Fe/O are measured to be [approx]2, [approx]2, and <0.3 times the solar values. Our spatially resolved spectral analysis from the northeastern rim to the western rim otherwise reveals sub-solar abundances consistent with those in the interstellar medium. The filament is coincident with several optically emitting O-rich knots with high velocities. If these are physically related, the filament would be a peculiar fragment of ejecta. On the other hand, the morphology of the filament suggests that it may trace ejecta heated by a shock reflected strongly off the dense ambient clouds near the northeastern rim.

Katsuda, Satoru↗
NASA NTRS2016

Continued Development of In Situ Geochronology for Planetary Missions Using KArLE (Potassium-Argon-Laser Experiment)

Since these techniques are very new and as they have never been used or this purpose. they will need to be replicated by several independent studies. These techniques may be very important if the optical imaging encounters difficulties, for example, if a sample is made of very dark or monochromatic material and in the case of very deep pits (>500 microns) Based on the preliminary results, the LIBS continuum technique is more appropriate to the large pits produced by long ablations The relationship may work best homogeneous samples, but the continuum is collected with every LIBS analysis so does not require any addition to the experimental suite of techniques. The integration of a QCMB in the ablation chamber may be a very interesting solution to determine the ablated mass. Even if it only measures a fraction of the total mass, its sensitivity should be able to weigh hundreds of nanograms accumulated on the crystal during ablation and relate it to the actual ablated mass. In the future. these options may help in situ K-Ar dating to give the age of the rock with the best accuracy and precision.

KArLE↗
NASA NTRS2019

Properties of Rubble-Pile Asteroid (101955) Bennu from OSIRIS-REx Imaging and Thermal Analysis

Establishing the abundance and physical properties of regolith and boulders on asteroids is crucial for understanding the formation and degradation mechanisms at work on their surfaces. Using images and thermal data from NASA's Origins, Spectral Interpretation, Resource Identification, and Security-Regolith Explorer (OSIRIS-REx) spacecraft, we show that asteroid (101955) Bennu's surface is globally rough, dense with boulders, and low in albedo. The number of boulders is surprising given Bennu's moderate thermal inertia, suggesting that simple models linking thermal inertia to particle size do not adequately capture the complexity relating these properties. At the same time, we find evidence for a wide range of particle sizes with distinct albedo characteristics. Our findings imply that ages of Bennu's surface particles span from the disruption of the asteroid's parent body (boulders) to recent in situ production (micrometre-scale particles).

DellaGiustina, D. N.↗
NASA NTRSDate not supplied

Chronology of Steno Crater Basalt

The Apollo Next Generation Sample Analysis (ANGSA) program was designed to investigate specially curated samples returned by the Apollo 15 and 17 missions [1]. As part of this effort, we are examining a suite of Apollo 17 basalts collected from the rim of Steno Crater, to investigate the magmatic and post-magmatic volatile histories of these samples. Here we report initial 207Pb-206Pb chronology of accessory phases for two steno crater basalts, which will ultimately be paired with (40)Ar*/(39)Ar chronology and (38)Ar exposure ages to constrain crystallization and surface histories. The chronology of these samples will help determine if the samples derive from the one lava sequence or represent multiple lava flows, and will allow us to place petrologic and volatile data into the wider context of the magmatic suite [1,2]. Note: Extended abstract on document.

S. J. Pomeroy↗
DOE OSTI2023

Comparative Analysis of Hotspot Stress Endurance in Pristine and Thermal Cycled Prestressed Glass–Glass Photovoltaic Modules

Hotspots pose a significant long-term reliability challenge in photovoltaic (PV) modules that can have a detrimental impact on the efficiency, safety, and financial viability of a PV system. This paper aims to evaluate the endurance of hotspot stress in pristine and prestressed glass–glass (GG) modules. The accelerated prestressing was conducted for 600 thermal cycles (TC600) to represent decades of field exposure. GG modules are quickly becoming an alternative to the traditional glass–backsheet (GB) modules that have been the industry standard. Unlike other conventional studies that subject only pristine modules to hotspot stress, this paper evaluates the performance of an accelerated/simulated field-aged GG module (using TC600) and a pristine GG module. Pre- and post-characterizations were performed before and after each test to determine changes in electrical performance and observe any defects in GG modules. During the hotspot test, an approximately 200 °C maximum cell temperature was observed with a cell shading of 25% (the worst-case shading ratio). After the hotspot test, electroluminescence imaging indicated that most cells in the prestressed GG module exhibited severe damage whereas no significant defects were evident in the pristine GG module where the prestressed GG module degraded 8.2% and the pristine GG module degraded 1.5% in maximum power. These findings are critical for the industry, considering that GG bifacial modules will dominate the market.

14 SOLAR ENERGY↗
DOE OSTI2021

The Effects of Herbarium Specimen Characteristics on Short-Read NGS Sequencing Success in Nearly 8000 Specimens: Old, Degraded Samples Have Lower DNA Yields but Consistent Sequencing Success

Phylogenetic datasets are now commonly generated using short-read sequencing technologies unhampered by degraded DNA, such as that often extracted from herbarium specimens. The compatibility of these methods with herbarium specimens has precipitated an increase in broad sampling of herbarium specimens for inclusion in phylogenetic studies. Understanding which sample characteristics are predictive of sequencing success can guide researchers in the selection of tissues and specimens most likely to yield good results. Multiple recent studies have considered the relationship between sample characteristics and DNA yield and sequence capture success. Here we report an analysis of the relationship between sample characteristics and sequencing success for nearly 8,000 herbarium specimens. This study, the largest of its kind, is also the first to include a measure of specimen quality (“greenness”) as a predictor of DNA sequencing success. We found that taxonomic group and source herbarium are strong predictors of both DNA yield and sequencing success and that the most important specimen characteristics for predicting success differ for DNA yield and sequencing: greenness was the strongest predictor of DNA yield, and age was the strongest predictor of proportion-on-target reads recovered. Surprisingly, the relationship between age and proportion-on-target reads is the inverse of expectations; older specimens performed slightly better in our capture-based protocols. We also found that DNA yield itself is not a strong predictor of sequencing success. Most literature on DNA sequencing from herbarium specimens considers specimen selection for optimal DNA extraction success, which we find to be an inappropriate metric for predicting success using next-generation sequencing technologies.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1977

The surface abundance and stratigraphy of lunar rocks from data about their albedo

The data pf ground-based studies and surveys of the lunar surface by the Zond and Apollo spacecraft have been used to construct an albedo map covering 80 percent of the lunar sphere. Statistical analysis of the distribution of areas with various albedos shows several types of lunar surface. Comparison of albedo data for maria and continental areas with the results of geochemical orbital surveys allows the identification of the types of surface with known types of lunar rock. The aluminum/silcon and magnesium/silicon ratios as measured by the geochemical experiments on the Apollo 15 and Apollo 16 spacecraft were used as an indication of the chemical composition of the rock. The relationship of the relative aluminum content to the age of crystalline rocks allows a direct dependence to be constructed between the mean albedo of areas and the age of the rocks of which they are composed.

Shevchenko, V. V.↗