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At least 1,495 records · Page 83

Deletion of the EP402R Gene from the Genome of African Swine Fever Vaccine Strain ASFV-G-ΔI177L Provides the Potential Capability of Differentiating between Infected and Vaccinated Animals

The African swine fever virus (ASFV) mutant ASFV-G-ΔI177L is a safe and efficacious vaccine which induces protection against the challenge of its parental virus, the Georgia 2010 isolate. Although a genetic DIVA (differentiation between infected and vaccinated animals) assay has been developed for this vaccine, still there is not a serological DIVA test for differentiating between animals vaccinated with ASFV-G-ΔI177L and those infected with wild-type viruses. In this report, we describe the development of the ASFV-G-ΔI177L mutant having deleted the EP402R gene, which encodes for the viral protein responsible for mediating the hemadsorption of swine erythrocytes. The resulting virus, ASFV-G-ΔI177L/ΔEP402R, does not have a decreased ability to replicates in swine macrophages when compared with the parental ASFV-G-ΔI177L. Domestic pigs intramuscularly (IM) inoculated with either 10 2 or 10 6 HAD 50 of ASFV-G-ΔI177L/ΔEP402R remained clinically normal, when compared with a group of mock-vaccinated animals, indicating the absence of residual virulence. Interestingly, an infectious virus could not be detected in the blood samples of the ASFV-G-ΔI177L/ΔEP402R-inoculated animals in either group at any of the time points tested. Furthermore, while all of the mock-inoculated animals presented a quick and lethal clinical form of ASF after the intramuscular inoculation challenge with 10 2 HAD 50 of highly virulent parental field isolate Georgia 2010 (ASFV-G), all of the ASFV-G-ΔI177L/ΔEP402R-inoculated animals were protected, remaining clinically normal until the end of the observational period. Most of the ASFV-G-ΔI177L/ΔEP402R-inoculated pigs developed strong virus-specific antibody responses against viral antigens, reaching maximum levels at 28 days post inoculation. Importantly, all of the sera collected at that time point in the ASFV-G-ΔI177L/ΔEP402R-inoculated pigs did not react in a direct ELISA coated with the recombinant EP402R protein. Conversely, the EP402R protein was readily recognized by the pool of sera from the animals immunized with recombinant live attenuated vaccine candidates ASFV-G-ΔI177L, ASFV-G-ΔMGF, or ASFV-G-Δ9GL/ΔUK. Therefore, ASFV-G-ΔI177L/ΔEP402R is a novel, safe and efficacious candidate with potential to be used as an antigenically DIVA vaccine.

59 BASIC BIOLOGICAL SCIENCES↗

Evaluation of Cross-Protection of African Swine Fever Vaccine ASFV-G-ΔI177L Between ASFV Biotypes

Background/Objectives: Vaccine development for the prevention of ASF has been very challenging due to the extensive genetic and largely unknown antigenic diversity. Inactivated vaccines, using different inactivation methods and a variety of adjuvants, have been consistently inefficacious. Historically, animals recovering from an infection with an attenuated virus became protected from the development of a clinical disease caused by an antigenically related strain. Therefore, immunization of susceptible animals with attenuathe ted virus strains has become a common method of vaccination with the first two commercially available vaccines based on recombinant live-attenuated viruses (LAVs). An important limitation is that the efficacy of the LAV is restricted to those strains that are antigenically related and, in most cases, only provide protection against homologous strains. Due to the unknown antigenic heterogeneity among all ASFV field isolates, the development of broad-spectrum vaccines is a challenge. Besides the anecdotal data, there is not a large amount of information describing patterns of cross-protection between different ASFV strains. Methods: We evaluated the cross-protection induced by the ASFV live-attenuated vaccine ASFV-G-ΔI177L against different biotypes of ASFV and compared their genomic sequences to determine potential genetic mutations that could cause the lack of cross-protection. Results: Results presented here demonstrate different patterns of protection when ASFV-G-ΔI177L vaccinated pigs were challenged with six different ASFV field isolates belonging to different biotypes. Conclusions: The presence of cross-protection cannot be predicted solely by the classical methodology for genotyping-based B646L ORF only. Biotyping, considering the entire virus proteome, appears to be a more promising prediction tool, although additional gathering of experimental data will be necessary to fully validate it; until then, the presence of cross-protection needs to be confirmed in efficacy trials challenging vaccinated animals.

Immunology↗

The Most Metal-poor Stars in the Magellanic Clouds Are r-process Enhanced*

The chemical abundances of a galaxy’s metal-poor stellar population can be used to investigate the earliest stages of its formation and chemical evolution. The Magellanic Clouds are the most massive of the Milky Way’s satellite galaxies and are thought to have evolved in isolation until their recent accretion by the Milky Way. Unlike the Milky Way’s less massive satellites, little is known about the Magellanic Clouds’ metal-poor stars. We have used the mid-infrared metal-poor star selection of Schlaufman & Casey and archival data to target nine LMC and four SMC giants for high-resolution Magellan/MIKE spectroscopy. These nine LMC giants with -2.4 ≲ [Fe/H] ≲ -1.5 and four SMC giants with -2.6 ≲ [Fe/H] ≲ -2.0 are the most metal-poor stars in the Magellanic Clouds yet subject to a comprehensive abundance analysis. While we find that at constant metallicity these stars are similar to Milky Way stars in their α, light, and iron-peak elemental abundances, both the LMC and SMC are enhanced relative to the Milky Way in the r-process element europium. These abundance offsets are highly significant, equivalent to 3.9σ for the LMC, 2.7σ for the SMC, and 5.0σ for the complete Magellanic Cloud sample. We propose that the r-process enhancement of the Magellanic Clouds’ metal-poor stellar population is a result of the Magellanic Clouds’ isolated chemical evolution and long history of accretion from the cosmic web combined with r-process nucleosynthesis on a timescale longer than the core-collapse supernova timescale but shorter than or comparable to the thermonuclear (i.e., Type Ia) supernova timescale.

79 ASTRONOMY AND ASTROPHYSICS↗

The Arecibo Galaxy Environment Survey. XII. Optically Dark H i Clouds in the Leo I Group

Using data from the Arecibo Galaxy Environment Survey, we report the discovery of five H i clouds in the Leo I group without detected optical counterparts. Three of the clouds are found midway between M96 and M95, one is only from the southeast side of the well-known Leo Ring, and the fifth is relatively isolated. H i masses range from 2.6 × 10 6 M ⊙ to 9.0 × 10 6 M ⊙ , and velocity widths (W50) range from 16 to 42 km s -1 . Although a tidal origin is the most obvious explanation, this formation mechanism faces several challenges. For the most isolated cloud, the difficulties are its distance from neighboring galaxies and the lack of any signs of disturbance in the H i disks of those systems. Some of the clouds also appear to follow the baryonic Tully–Fisher relation between mass and velocity width for normal, stable galaxies, which is not expected if they are tidal in origin. Three clouds are found between M96 and M95 that have no optical counterparts but have otherwise similar properties and locations to the optically detected galaxy LeG 13. While overall we favor a tidal debris scenario to explain the clouds, we cannot rule out a primordial origin. If the clouds were produced in the same event that gave rise to the Leo Ring, they may provide important constraints on any model attempting to explain that structure.

79 ASTRONOMY AND ASTROPHYSICS↗

Galactic Chemical Evolution of Short-lived Radioactive Isotopes Produced by Explosive Nucleosynthesis: 60 Fe and 53 Mn

Several short-lived radionuclides (SLRs) are know to have existed in the early solar system (ESS). These species, which typically decay with half-lives of the order of a few million years, can be used to probe the timescales of events preceding the birth of the Sun. We investigate the ESS origin of 53 Mn, produced by core-collapse supernovae (CCSNe) and Type Ia supernovae (SNe Ia), and 60 Fe, produced exclusively by CCSNe. We model the evolution of the radioactive-to-stable abundance ratios of these SLRs with a Galactic chemical evolution (GCE) framework accounting for different SN yields, SN Ia delay times, and other Galactic features (K). A further set of models is calculated assuming that SNe Ia did not contribute any 53 Mn to the ESS. The predicted ratios are compared to meteoritic ratios to derive a distribution of solar isolation times that includes uncertainties due to stochastic chemical enrichment and measurements of the ESS values. The isolation times are then compared to those of 107 Pd and 182 Hf calculated in previous work. A self-consistent solution can be found within the current uncertainties, especially when using the GCE setups with K = 1.6 and 2.3, although the maximum likelihood for the 60 Fe distribution is typically ∼4–5 Myr shorter than for 53 Mn. The predicted 60 Fe/ 53 Mn ratio, instead, is completely inconsistent with the ESS value; this could be resolved using a larger fraction of faint CCSNe than usually considered in GCE models.

79 ASTRONOMY AND ASTROPHYSICS↗

Hard X-Ray Excess from the Magnificent Seven Neutron Stars

We report significant hard X-ray excesses in the energy range 2–8 keV for two nearby isolated neutron stars: RX J1856.6-3754 and RX J0420.0-5022. These neutron stars have previously been observed in soft X-rays to have nearly thermal spectra at temperatures ~100 eV, which are thought to arise from the warm neutron star surfaces. We find nontrivial hard X-ray spectra well above the thermal surface predictions with archival data from the XMM-Newton and Chandra X-ray telescopes. We analyze possible systematic effects that could generate such spurious signals, such as nearby X-ray point sources and pileup of soft X-rays, but we find that the hard X-ray excesses are robust to these systematics to the extent that is possible to test. We also investigate possible sources of hard X-ray emission from the neutron stars and find no satisfactory explanation with known mechanisms, suggesting that a novel source of X-ray emission is at play. Here, we do not find high-significance hard X-ray excesses from the other five Magnificent Seven isolated neutron stars.

79 ASTRONOMY AND ASTROPHYSICS↗

On the H I Content of MaNGA Major Merger Pairs

The role of H I content in galaxy interactions is still under debate. To study the H I content of galaxy pairs at different merging stages, we compile a sample of 66 major-merger galaxy pairs and 433 control galaxies from the Sloan Digital Sky Survey IV (SDSS-IV) MaNGA IFU survey. In this study, we adopt kinematic asymmetry as a new effective indicator to describe the merging stage of galaxy pairs. With archival data from the HI-MaNGA survey and new observations from the Five-hundred-meter Aperture Spherical radio Telescope (FAST), we investigate the differences in H I gas fraction (f H I ), star formation rate (SFR), and H I star formation efficiency (SFE H I ) between the pair and control samples. Our results suggest that the H I gas fraction of major-merger pairs on average is marginally decreased by ~15% relative to isolated galaxies, implying mild H I depletion during galaxy interactions. Compared to isolated galaxies, pre-passage paired galaxies have similar f H I , SFR, and SFE H I , while pairs during the pericentric passage have weakly decreased f H I (–0.10 ± 0.05 dex), significantly enhanced SFR (0.42 ± 0.11 dex), and SFE H I (0.48 ± 0.12 dex). When approaching the apocenter, paired galaxies show marginally decreased f H I (–0.05 ± 0.04 dex), comparable SFR (0.04 ± 0.06 dex), and SFE H I (0.08 ± 0.08 dex). We propose that the marginally detected H i depletion may originate from the gas consumption in fueling the enhanced H2 reservoir of galaxy pairs. In addition, new FAST observations also reveal a H I absorber (N H I ~ 4.7 × 10 21 cm –2 ), which may suggest gas infalling and the triggering of active galactic nuclei activity.

79 ASTRONOMY AND ASTROPHYSICS↗

SDSS-IV MaNGA: How Galaxy Interactions Influence Active Galactic Nuclei

We present a comparative study of active galactic nuclei (AGN) between galaxy pairs and isolated galaxies with the final data release of the MaNGA integral field spectroscopic survey. We build a sample of 391 kinematic galaxy pairs within the footprint of the survey and select AGN using the survey's spectra. We use the comoving volume densities of the AGN samples to quantify the effects that tidal interactions have on the triggering of nuclear accretion. Our hypothesis is that the pair sample contains AGN that are triggered by not only stochastic accretion but also tidally induced accretion and correlated accretion. With the level of stochastically triggered AGN fixed by the control sample, we model the strength of tidally induced accretion and correlated accretion as a function of projected separation (r p ) and compare the model expectations with the observed volume densities of dual AGN and offset AGN (single AGN in a pair). At r p ~ 10 kpc, we find that tidal interactions induce ~30% more AGN than stochastic fueling and cause ~12% of the offset AGN to become dual AGN because of correlations. The strength of both these effects decreases with increasing r p . We also find that the [O III ] luminosities of the AGN in galaxy pairs are consistent with those found in isolated galaxies, likely because stochastically fed AGN dominate even among close pairs. Our results illustrate that while we can detect tidally induced effects statistically, it is challenging to separate tidally induced AGN and stochastically triggered AGN in interacting galaxies.

79 ASTRONOMY AND ASTROPHYSICS↗

Flares, Warps, Truncations, and Satellite: The Ultra-thin Galaxy UGC 11859

The structure of the outskirts of galaxies provides valuable information about their past and evolution. Due to their projected orientation, edge-on isolated galaxies effectively serve as test labs in which to study the three-dimensional structures of galaxies, including warps and flares, and to explore the possible sources of such distortions. We analyzed the structure of the apparently isolated edge-on ultra-thin galaxy UGC 11859 to look for the presence of distortions. The deep optical imaging observations (${\mu }_{\mathrm{lim}}=30.6$ and 30.0 $\mathrm{mag}\ {\mathrm{arcsec}}^{-2}$ in the g- and r-bands, respectively) we acquired with the 10.4 m Gran Telescopio Canarias are used to derive the radial and vertical surface brightness profiles and g - r color radial profile. We find that UGC 11859's disk displays a significant gravitational distortion. A warp is clearly detected on one side of the disk, and the galactic plane on both sides of the center shows increasing scale height with an increasing galactocentric radius, indicating the presence of a flare in the stellar distribution. The surface brightness profile of the disk shows a sharp break at 24 kpc galactocentric radius, and a steep decline to larger radii, an "edge-on truncation," which we associate with the presence of the flare. The present study is the first observational support for a connection between truncations and flares. Just beyond the warped side of the disk, a faint galaxy is observed within a small angular distance, identified as a potential interacting companion. Based on ultradeep g and r photometry we estimate that if the potential companion is at the same distance as UGC 11859, the stellar mass of the satellite galaxy is log 10 (M ⊙ ) = ${6.33}_{-0.02}^{+0.02}$ .

79 ASTRONOMY AND ASTROPHYSICS↗

Spindown of Pulsars Interacting with Companion Winds: The Impact of Magnetospheric Compression

Pulsars in binary systems with strong companion winds can have the magnetopause separating their magnetosphere from the wind located well within their light cylinder. This bow-like enclosure effectively creates a waveguide that confines the pulsar's electromagnetic fields and can significantly alter its spindown. In this paper, we study the spindown of compressed pulsar magnetospheres in such systems. We parameterize the confinement as the ratio between the equatorial position of the magnetopause (or standoff distance) R m and the pulsar's light cylinder R LC . Using particle-in-cell simulations, we quantify the pulsar spindown for a range of compressions, R m /R LC = 1/3–1, and inclination angles, χ = 0°...90°, between magnetic and rotation axes. Our strongly confined models (R m /R LC = 1/3) show two distinct limits. For χ = 0°, the spindown of a compressed pulsar magnetosphere is enhanced by approximately a factor of three compared to an isolated pulsar due to the increased number of open magnetic field lines. Conversely, for χ = 90°, the compressed pulsar spins down at less than 40% of the rate of an isolated reference pulsar due to the mismatch between the pulsar wind stripe wavelength and the waveguide size. We apply our analysis to the 2.77 s oblique rotator (χ = 60°) in the double-pulsar system PSR J0737-3039. With the numerically derived spindown estimate, we constrain its surface magnetic field to B* ≈ (7.3 ± 0.2) × 10 11 G. We discuss the time modulation of its period derivative, the effects of compression on its braking index, and implications for the radio eclipse in PSR J0737-3039.

79 ASTRONOMY AND ASTROPHYSICS↗

H I Gas and Star Formation in Major Galaxy Pairs from the FAST All Sky H I Survey (FASHI)

Atomic hydrogen (H I ) plays a fundamental role in fueling star formation in galaxies. However, the behavior of H I gas in interacting systems, particularly galaxy pairs, remains elusive. In this work, we investigate the H I content of major mergers by crossmatching the extragalactic H I catalog from the Five-hundred-meter Aperture Spherical radio Telescope (FAST) All-Sky H I Survey with a previously established sample of isolated galaxy pairs. With the superior sensitivity of FAST, we have constructed the largest sample of major mergers with H I detections, consisting of 440 galaxy pairs: 364 spiral-spiral (S+S) and 76 spiral-elliptical (S+E) systems. We examine the H I gas fraction (f H I ), star formation rate (SFR), and H I star formation efficiency (SFE H I = SFR/M H I ) for individual galaxies in pairs. The control sample is matched in both stellar mass and redshift. We find that paired galaxies, particularly those in pairs with small projected separations (d p < 50 h −1 kpc), exhibit systematically lower (by 8.8%) H I gas fractions compared to the control galaxies. The SFR is enhanced for galaxies in the S+S pairs. The SFE H I is ∼15% higher for galaxies in the S+S pairs than in the control galaxies, while spiral galaxies in the S+E pairs show no significant difference in SFE H I compared to the control sample. These findings suggest that the merger process triggers efficient H I gas depletion and enhances star formation, especially in close S+S pairs. Notably, our sample includes 26 red spirals in paired systems. These galaxies exhibit H I deficiency and suppressed star formation activity compared to the isolated galaxies, indicating that interactions may affect quiescent spirals differently, potentially due to mechanisms similar to those of ellipticals.

Yan, Shulan 淑澜鄢 [Xiamen University (China); SDSS C↗

A Self-interacting Dark Matter Solution to the Extreme Diversity of Low-mass Halo Properties

Abstract The properties of low-mass dark matter (DM) halos appear to be remarkably diverse relative to cold, collisionless DM predictions, even in the presence of baryons. We show that self-interacting DM (SIDM) can simultaneously explain observations of halo diversity at two opposite extremes—the inner density profile of the dense substructure perturbing the strong lens galaxy SDSSJ0946+1006 and the rotation curves of isolated, gas-rich ultradiffuse galaxies (UDGs). To achieve this, we present the first cosmological zoom-in simulation featuring strong DM self-interactions in a galaxy group environment centered on a 10 13 M ⊙ host halo. In our SIDM simulation, most surviving subhalos of the group-mass host are deeply core-collapsed, yielding excellent candidates for the observed dense strong-lens perturber. Self-interactions simultaneously create kiloparsec-scale cores in low-concentration isolated halos, which could host the observed UDGs. Our scenario can be further tested with observations of DM structure and galaxies over a wide mass range.

Astronomy & Astrophysics↗

Developing Fuel Cell Electric Powertrain Architectures for Commercial Vehicles

Here, this article addresses the architecture development for a commercial vehicle fuel cell electric powertrain by establishing a clear multi-step formalized workflow that employs a unique technoeconomic solution for architecture selection. The power capability of the fuel cell, the energy capacity and chemistry of the electrical energy storage (battery), the DC-DC converter (including the input current rating and isolation resistance requirements), the traction drive solution, the on-board hydrogen storage solution, and the real-time power-split management of the fuel cell and the battery are all considered and developed in this effort. The methods were used to select architecture for Class 8 urban, regional, and line haul applications. When compared to traditional load-following power-split controllers, an energy management power-split controller can increase system energy efficiency by up to 19.5%. The energy-efficient power-split controller may increase the required battery capacity for an equivalent life by up to 2.6 times. The impact on the total cost of ownership (TCO) for a variety of financial cases demonstrates that high C-rate capable batteries have the potential to provide better TCO solutions over a six-year vehicle life than low C-rate capable batteries. To achieve TCO parity with the 600 A non-isolated DC-DC converter case, the specific choice of the fuel cell DC-DC converter to achieve a target power output based on current levels (from 500 A to 2400 A) shows that efficiency decreases and cost increases due to the higher current, requiring fuel cell prices to decrease by $50–$100/kW, $60–$110/kW, and $100–$220/kW for urban, regional, and line haul applications, respectively. Key recommendations for powertrain system architectures are provided, with specifics based on vehicle dynamics, mission and application characteristics, end customer use-case profile, critical powertrain component costs, and architecture selection cost function. This study rigorously demonstrates the interplay of the above parameters, with a focus on TCO, and provides application decision-makers with a mechanism and well-defined set of impact factors to consider as part of their architecture selection process.

25 ENERGY STORAGE↗

Effects of Port Angle on Scavenging of an Opposed Piston Two-Stroke Engine

Opposed-piston 2-stroke (OP-2S) engines have the potential to achieve higher thermal efficiency than a typical diesel engine. However, the uniflow scavenging process is difficult to control over a wide range of speeds and loads. Scavenging performance is highly sensitive to pressure dynamics, port timings, and port design. This study proposes an analysis of the effects of port vane angle on the scavenging performance of an opposed-piston 2-stroke engine via simulation. A CFD model of a three-cylinder opposed-piston 2-stroke was developed and validated against experimental data collected by Achates Power Inc. One of the three cylinders was then isolated in a new model and simulated using cycle-averaged and cylinder-averaged initial/ boundary conditions. This isolated cylinder model was used to efficiently sweep port angles from 12 degrees to 29 degrees at different pressure ratios. Results indicate that scavenging performance is correlated with the bulk swirl ratio generated by these port angles. Scavenging performance is also sensitive to the pressure ratio across the engine. It was concluded that, for a given pressure ratio, the smallest port angle produces the best scavenging; however, previous work indicates lower bulk swirl ratio during combustion can have an impact on emissions formation.

33 ADVANCED PROPULSION SYSTEMS↗

Revisiting adiabatic fraction estimations in cumulus clouds: high-resolution simulations with a passive tracer

The process of mixing in warm convective clouds and its effects on microphysics are crucial for an accurate description of cloud fields, weather, and climate. Still, they remain open questions in the field of cloud physics. Adiabatic regions in the cloud could be considered non-mixed areas and therefore serve as an important reference to mixing. For this reason, the adiabatic fraction (AF) is an important parameter that estimates the mixing level in the cloud in a simple way. Here, we test different methods of AF calculations using high-resolution (10 m) simulations of isolated warm cumulus clouds. The calculated AFs are compared with a normalized concentration of a passive tracer, which is a measure of dilution by mixing. This comparison enables the examination of how well the AF parameter can determine mixing effects and the estimation of the accuracy of different approaches used to calculate it. Comparison of three different methods to derive AF, with the passive tracer, shows that one method is much more robust than the others. Moreover, this method's equation structure also allows for the isolation of different assumptions that are often practiced when calculating AF such as vertical profiles, cloud-base height, and the linearity of AF with height. The use of a detailed spectral bin microphysics scheme allows an accurate description of the supersaturation field and demonstrates that the accuracy of the saturation adjustment assumption depends on aerosol concentration, leading to an underestimation of AF in pristine environments.

54 ENVIRONMENTAL SCIENCES↗

Inferring and evaluating satellite-based constraints on NO x emissions estimates in air quality simulations

Satellite observations of tropospheric NO 2 columns can provide top-down observational constraints on emissions estimates of nitrogen oxides (NO x ). Mass-balance-based methods are often applied for this purpose but do not isolate near-surface emissions from those aloft, such as lightning emissions. Here, we introduce an inverse modeling framework that couples satellite chemical data assimilation to a chemical transport model. In the framework, satellite-constrained emissions totals are inferred using model simulations with and without data assimilation in the iterative finite-difference mass-balance method. The approach improves the finite-difference mass-balance inversion by isolating the near-surface emissions increment. We apply the framework to separately estimate lightning and anthropogenic NO x emissions over the Northern Hemisphere for 2019. Using overlapping observations from the Ozone Monitoring Instrument (OMI) and the Tropospheric Monitoring Instrument (TROPOMI), we compare separate NO x emissions inferences from these satellite instruments, as well as the impacts of emissions changes on modeled NO 2 and O 3 . OMI inferences of anthropogenic emissions consistently lead to larger emissions than TROPOMI inferences, attributed to a low bias in TROPOMI NO 2 retrievals. Updated lightning NO x emissions from either satellite improve the chemical transport model's low tropospheric O 3 bias. The combined lighting and anthropogenic emissions updates improve the model's ability to reproduce measured ozone by adjusting natural, long-range, and local pollution contributions. Thus, the framework informs and supports the design of domestic and international control strategies.

54 ENVIRONMENTAL SCIENCES↗

Aerosol-deep convection interaction based on joint cell-thermal tracking in Large Eddy Simulations during the TRACER campaign

In cumulus clouds, aerosol concentrations control cloud droplet concentrations, modifying cloud radiative properties, precipitation processes, and cloud electrification. However, mechanisms of aerosol-deep convection interactions are not well understood due to complex cloud dynamics and microphysics. We investigate the interaction of aerosols with isolated deep convection using Large Eddy Simulations of two cases during the TRacking Aerosol Convection interactions ExpeRiment (TRACER) near Houston, Texas, using a joint cell-thermal tracking algorithm. Cumulus thermals are droplet generators, since supersaturation and droplet nucleation coincide with thermal centers, where the strongest updrafts occur. Primary ice crystal formation does not take place inside thermals, but at layers where previous thermals detrained moisture. As subsequent thermals containing supercooled droplets penetrate these layers, hail and graupel form at or near these thermals. Higher aerosol concentrations result in higher droplet concentrations that suppress drizzle, delay warm rain processes, and transport more moisture aloft. This increases snow and ice amount, as well as graupel and hail, leading to more lightning. Polluted thermals initiate at slightly higher altitudes, and are slightly larger and faster, suggesting a weak invigoration. We also find more thermals per cell, but fewer isolated cells, since convection is more aggregated and intense, especially near the end of the 24 h simulation. Non-linear mesoscale feedback likely triggered by temperature and moisture responses to aerosol-thermal interactions causes the aggregation. Time-lagged aerosol-reinitialization experiments show that the mesoscale response is the predominant forcing for the invigoration. These changes happen within one day, on a smaller scale than previously suggested.

54 ENVIRONMENTAL SCIENCES↗

Diverse organic carbon dynamics captured by radiocarbon analysis of distinct compound classes in a grassland soil

Soil organic carbon (SOC) is a large, dynamic reservoir composed of a complex mixture of plant- and microbe-derived compounds with a wide distribution of cycling timescales and mechanisms. The distinct residence times of individual carbon components within this reservoir depend on a combination of factors, including compound reactivity, mineral association, and climate conditions. To better constrain SOC dynamics, bulk radiocarbon measurements are commonly used to trace biosphere inputs into soils and to estimate timescales of SOC cycling. However, understanding the mechanisms driving the persistence of organic compounds in bulk soil requires analyses of SOC pools that can be linked to plant sources and microbial transformation processes. Here, we adapt approaches, previously developed for marine sediments, to isolate organic compound classes from soils for radiocarbon ( 14 C) analysis. We apply these methods to a soil profile from an annual grassland in Hopland, California (USA), to assess changes in SOC persistence with depth (down to 1 m). We measured the radiocarbon values of water-extractable organic carbon (WEOC), total lipid extracts (TLEs), total hydrolyzable amino acids (AAs), and an acid-insoluble (AI) fraction from bulk and physically separated size fractions (< 2 mm, 2 mm–63 µm, and < 63 µm). Our results show that Δ 14 C values of bulk soil, size fractions, and extracted compound classes became more depleted with depth, and individual SOC components have distinct age–depth distributions that suggest distinguishable cycling rates. We found that AAs and TLEs cycle faster than the bulk soils and the AI fraction. The AI was the most 14 C-depleted fraction, indicating that it is the most chemically inert in this soil. Our approach enables the isolation and measurement of SOC fractions that separate functionally distinct SOC pools that can cycle relatively quickly (e.g., plant and microbial residues) from more passive or inert SOC pools (associated with minerals or petrogenic) from bulk soils and soil physical fractions. With the effort to move beyond SOC bulk analysis, we find that compound class 14 C analysis can improve our understanding of SOC cycling and disentangle the physical and chemical factors driving OC cycling rates and persistence.

58 GEOSCIENCES↗