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At least 1,495 records · Page 83

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DOE OSTI2021

Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT

We previously described X-ray histotomography, a high-resolution, non-destructive form of X-ray microtomography (micro-CT) imaging customized for three-dimensional (3D), digital histology, allowing quantitative, volumetric tissue and organismal phenotyping (Ding et al., 2019). Here, we have combined micro-CT with a novel application of ionic silver staining to characterize melanin distribution in whole zebrafish larvae. The resulting images enabled whole-body, computational analyses of regional melanin content and morphology. Normalized micro-CT reconstructions of silver-stained fish consistently reproduced pigment patterns seen by light microscopy, and further allowed direct quantitative comparisons of melanin content across wild-type and mutant samples, including subtle phenotypes not previously noticed. Silver staining of melanin for micro-CT provides proof-of-principle for whole-body, 3D computational phenomic analysis of a specific cell type at cellular resolution, with potential applications in other model organisms and melanocytic neoplasms. Advances such as this in whole-organism, high-resolution phenotyping provide superior context for studying the phenotypic effects of genetic, disease, and environmental variables.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS1969

Coherent optical data processing and remotely sensed imagery

It is shown that an automatic imaging system consisting of a combination of optical and digital computer elements is feasible and has considerable advantages over direct image scanning systems. With such a system, it is possible to process very large quantities of remotely sensed image spectra.

Macdougall, E. B.↗

NASA NTRS1973

Marshes and turbid waters in the French Atlantic littoral

The author has identified the following significant results. The multispectral information provided by ERTS-1 is very rich for the coastal regions but the cloud cover, even when only partial, often cuts up the data, and lessens its practical value. The research by densitometric methods has thus far been the most fruitful. It consists of measuring densities along preferential axes. This method has enabled the investigators to perfect a system of computer cartography for the best image. Comparative study of microdensitometric transects in four MSS bands for carefully dilimited profiles on the ground, makes it possible to isolate and recognize various types of countryside: (1) countryside of coastal plains; (2) countryside of coastal sand dunes and beaches; and (3) forms and processes of offshore domains. This study shows the usefulness of the ERTS program in establishing a rapid cartography of the physiographic units of the coastal plains in the interest of a rational program of exploitation and development.

Verger, F.↗

NASA NTRS1973

ERIM progress report on use of ERTS-1 data: Summary report of work on ten tasks

The author has identified the following significant results. Several of the tasks have produced significant results which are summarized: (1) Absolute water depth can be calculated from a ratio of signals from bands MSS 4 and MSS 5. (2) A 13 category terrain feature classification map of Yellowstone National Park has been produced using supervised pattern recognition techniques. (3) ERTS-1 data has been shown to provide a detection and monitoring capability for a number of water quality problems associated with off-shore ocean dumping sites and inland lakes. (4) A corrected ratio of bands MSS-5 and MSS-7 signals has been formed. (5) A concise format has been devised for storing the ratio signatures of geologic rock and mineral materials determined from laboratory reflectance spectra. (6) Results of work in information extraction demonstrate: signal variability exists among ERTS-1 detectors in any one spectral band that will impact users doing quantitative analysis on successive ERTS-1 images; a newly developed computer-aided procedure for correlating ERTS-1 pixels to ground features; the strong influence of atmospheric effects in ERTS-1 data; and area estimation accuracies are better using the ERIM proportion estimation algorithm than for conventional recognition techniques.

Thomson, F. J.↗

NASA NTRS1977

The application of digital techniques to the analysis of metallurgical experiments

The application of a specific digital computer system (known as the Image Data Processing System) to the analysis of three NASA-sponsored metallurgical experiments is discussed in some detail. The basic hardware and software components of the Image Data Processing System are presented. Many figures are presented in the discussion of each experimental analysis in an attempt to show the accuracy and speed that the Image Data Processing System affords in analyzing photographic images dealing with metallurgy, and in particular with material processing.

Rathz, T. J.↗

NASA NTRS1977

Toward fully automated agricultural inventory from satellites

Conventional approaches to inventories from satellites rely heavily on the judgment of image analysts, who 'train' the computer classification program. The fully automated approach to this problem, described in the present paper, eliminates the need for human judgment in training the computer, by using fully automated techniques for selecting spectral characteristics and classification parameters to optimize agreement with ground truth in specified test areas.

Ungar, S. G.↗

NASA NTRS1982

NORSEX 1979 microwave remote sensing data report

Airborne microwave remote sensing measurements obtained by NASA Langley Research Center in support of the 1979 Norwegian Remote Sensing Experiment (NORSEX) are summarized. The objectives of NORSEX were to investigate the capabilities of an active/passive microwave system to measure ice concentration and type in the vicinity of the marginal ice zone near Svalbard, Norway and to apply microwave techniques to the investigation of a thermal oceanic front near Bear Island, Norway. The instruments used during NORSEX include the stepped frequency microwave radiometer, airborne microwave scatterometer, precision radiation thermometer and metric aerial photography. The data are inventoried, summarized, and presented in a user-friendly format. Data summaries are presented as time-history plots which indicate when and where data were obtained as well as the sensor configuration. All data are available on nine-track computer tapes in card-image format upon request to the NASA Langley Technical Library.

Hennigar, H. F.↗

NASA NTRS1982

Remote sensing of agricultural crops and soils

Research results and accomplishments of sixteen tasks in the following areas are described: (1) corn and soybean scene radiation research; (2) soil moisture research; (3) sampling and aggregation research; (4) pattern recognition and image registration research; and (5) computer and data base services.

Bauer, M. E.↗

NASA NTRS1981

Oregon trails revisted

Oregon State University's Environmental Remote Sensing Applications Laboratory (ERSAL) has six full-time researchers with expertise in a variety of biological, Earth, atmospheric and computer sciences as well as image interpretation and statistical techniques. The primary emphasis of the ERSAL research and demonstration program is the development and application of remote sensing technology in operational resource management programs. LANDSAT multi-spectral, multi-date digital data and imagery are utilized in concert with high altitude NASA-acquired photography, low altitude ERSAL-acquired photography, and field observations and data to provide customized, inexpensive and useful final products. Synopses are given of 9 applications projects conducted in Oregon.

Lewis, A. J.↗

NASA NTRS1982

Cost effective development of a Shuttle-based astronomical instrument control system

The high level language FORTH is used for the electronic control of the Space Shuttle-based Ultraviolet Imaging Telescope, in a flight computer system which minimizes costs. The greater part of the breadboard version of the flight computer is assembled from commercially available components, reducing novel circuit design features and permitting simultaneous development of both hardware and software. The commercial boards are then refabricated on aluminum core heat conducting stock, using high reliability parts to produce the flight versions of the system. The system's ground support equipment employs a MINC-25 minicomputer which performs such functions as flight computer software development, PROM programming, test and integration support, and flight operations support. The implementation of these concepts in flight computer telescope controls is described.

Parise, R. A.↗

NASA NTRS1983

Environmental data display

Methods for using a combination of computer-generated color graphics and image processing techniques to display a large data base of environment information are described. The data source can be either field data or mathematical models, reduced to summary statistics that characterize the data field as a whole. Sharp gradients are plotted into contour plots, which can also feature shades, degree of brightness, and saturation levels for fine-tuning the image. The basic concepts of digital image processing are reviewed, including location of the pixels, intensity mapping operations, pseudocolor enhancements, neighborhood averaging, and smoothing. Sample applications are presented in terms of emissions and air quality distributions over the south coast air basin of southern California.

Hussey, K. J.↗

NASA NTRS1984

Research in solar physics: Some techniques for analyzing data from the ultraviolet spectrometer and polarimeter

Useful information for certain aspects of the analysis of data obtained by the Ultraviolet Spectrometer and Polarimeter (UVSP) on the Solar Maximum Mission (SMM) are contained. The meaning of the UVSPCO-ordinate system and the SMM roll, pitch, and yaw are described and the process for overlaying UVSP images is explained. The various computer programs that calculate the line of sight component of the SMM spacecraft velocity from the spacecraft to the Sun is described. The spacecraft velocity is used to correct or interpret the signal observed in USVP dopplergrams. A method of using the spacecraft velocity to calibrate UVSP dopplergrams and magnetograms, i.e., determine the width of the observed emission line is applied. The UVSP polarization analysis procedures are described and the expressions for the statistical uncertainties in various quantities obtained from UVSP measurements are given.

Henze, W., Jr.↗

NASA NTRS1985

Activities at the Lunar and Planetary Institute

The activities of the Lunar and Planetary Institute for the period July to December 1984 are discussed. Functions of its departments and projects are summarized. These include: planetary image center; library information center; computer center; production services; scientific staff; visitors program; scientific projects; conferences; workshops; seminars; publications and communications; panels, teams, committees and working groups; NASA-AMES vertical gun range (AVGR); and lunar and planetary science council.

Source record↗

NASA NTRS1985

Spatial distribution read-out system for thermoluminescence sheets

A spatial distribution read-out system of thermoluminescence (TL) sheets is developed. This system consists of high gain image intensifier, a CCD-TV camera, a video image processor and a host computer. This system has been applied to artificial TL sheets (BaSO4:Eu doped) for detecting high energy electromagnetic shower and heavy nuclei tracks.

Yamamoto, I.↗

NASA NTRS1984

MIDAS - A microcomputer-based image display and analysis system with full Landsat frame processing capabilities

Image Display and Analysis Systems (MIDAS) developed at NASA/Ames for the analysis of Landsat MSS images is described. The MIDAS computer power and memory, graphics, resource-sharing, expansion and upgrade, environment and maintenance, and software/user-interface requirements are outlined; the implementation hardware (including 32-bit microprocessor, 512K error-correcting RAM, 70 or 140-Mbyte formatted disk drive, 512 x 512 x 24 color frame buffer, and local-area-network transceiver) and applications software (ELAS, CIE, and P-EDITOR) are characterized; and implementation problems, performance data, and costs are examined. Planned improvements in MIDAS hardware and design goals and areas of exploration for MIDAS software are discussed.

Hofman, L. B.↗

NASA NTRS1983

Improving our understanding of the remote sensing process

Plans for two fundamental research programs aimed at improving an understanding of the physics and mathematics of remote sensing are discussed. The first program, Scene Radiation and Atmospheric Research Program, is concerned with developing the capability to determine biophysical attributes of terrestrial scenes through an improved understanding of the relationships between those properties and remotely sensed radiation. The research issues in the second program, Mathematical Pattern Recognition and Image Analysis, are discussed with reference to the following five categories: preprocessing, digital image representation, object scene inference, computational structures, and continuing studies.

Calabrese, M. A.↗

NASA NTRS1986

Comet Halley near-nucleus phenomena in 1986

Computer enhanced high resolution groundbased images taken during Halley's most active phases in 1985-86 exhibit all of the coma structures identified in 1910 photographs. The structures result mostly from discrete dust jet activity producing spirals, arcs, and jagged-edged envelopes. Multiple nucleus and antisunward jet phenomena are also observed. The curvature of the jets is consistent with the location of the Earth with respect to the nucleus equatorial plane. The variation in jet activity during the Vega and Giotto flybys is easily seen on the groundbased images. Images of CN jets show that they usually do not correlate with dust jets. Wide field, medium resolution images of faint outer envelopes are required to augment the smaller field, high resolution images of near-nucleus jets in modeling the jets.

Larson, S.↗

NASA NTRS1988

An interactive testbed for development of expert tools for pattern recognition

The initial implementation of an interactive testbed for development of expert system applications in image processing, i.e., a toolbox of procedures designed to facilitate the capture of expert knowledge for region grouping and analysis is described. The user can elect to interactively enter commands (via a command interpreter) for region manipulation to, in effect, simulate the actions of a hypothetical expert system. The user can then incorporate any rules and procedures as derived from interactive experimentation into customized region processing procedures using the library of utility functions. An iterative technique based on image pyramids is used to compute the initial region segmentation without the use of process parameters. These regions can then be interactively examined and manipulated using the command interpreter.

Wharton, Stephen W.↗