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At least 1,477 records · Page 82

Demonstration of a sterilizable solid rocket motor system

A solid propellant rocket motor containing 60.9 Kg (134-lb) of propellant was successfully static fired after being subjected to eight heat sterilization cycles (three 54-hour cycles plus five 40-hour cycles) at 125 C (257 F). The test motor, a modified SVM-3 chamber, incorporated a flexible grain retention system of EPR rubber to relieve thermal shrinkage stresses. The propellant used in the motor was ANB-3438, and 84 wt% solids system (18 wt% aluminum) containing 66 wt% stabilized ammonium perchlorate oxidizer and a saturated hydroxylterminated polybutadiene binder. Bonding of the propellant to the EPR insulation (GenGard V-4030) was provided by the use of SD-886, an epoxy urethane restriction.

Mastrolia, E. J.↗

Viking planetary quarantine procedures and implementation

Some of the techniques and methodology that were used on Viking to implement planetary quarantine requirements are reported. Special attention was given to techniques and approaches used to implement sterilization of the Viking probe. Quarantine procedures for unmanned planetary missions and procedures for microbiological contamination of space hardware are included. A probability of contamination of the biological instruments onboard by terrestrial organisms was examined.

Howell, R.↗

Automated microbial metabolism laboratory

The labeled release concept was advanced to accommodate a post- Viking mission designed to extend the search, to confirm the presence of, and to characterize any Martian life found, and to obtain preliminary information on control of the life detected. The advanced labeled release concept utilizes four test chambers, each of which contains either an active or heat sterilized sample of the Martian soil. A variety of C-14 labeled organic substrates can be added sequentially to each soil sample and the resulting evolved radioactive gas monitored. The concept can also test effects of various inhibitors and environmental parameters on the experimental response. The current Viking '75 labeled release hardware is readily adaptable to the advanced labeled release concept.

Source record↗

Ecology and thermal inactivation of microbes in and on interplanetary space vehicle components

A series of experiments was conducted to determine the dry heat resistance of microorganisms in soil obtained from Denver Colorado, Pasadena California, Kennedy Space Center Florida, and Cincinnati Ohio. The results of the KSC terminal sterilization cycle experiment are given in graphs. The average number of viable organisms per m1 was calculated for 18 replicate soil samples for each sample area and points plotted equivalent to 30 hr exposure at 112 C. The result showed a reduction of 3 logs from the initial population for both KSC and Cincinnati soil samples. Results from other areas are given in graphs.

Reyes, A. L.↗

Environmental microbiology as related to planetary quarantine

Continued experimental work related to dry heat resistance of microorganisms. One phase of this research has been concerned with the viability and dry heat resistance of indigenous microflora associated with small soil particles. The second part of this report is an analysis of the present status of dry heat sterilization. An attempt is made to integrate results for both laboratory grown spores and spores in soil.

Pflug, I. J.↗

Objectives and models of the planetary quarantine program

The objectives of the planetary quarantine program are presented and the history of early contamination prevention efforts is outlined. Contamination models which were previously established are given and include: determination of parameters; symbol nomenclature; and calculations of contamination and hazard probabilities. Planetary quarantine is discussed as an issue of national and international concern. Information on international treaty and meetings on spacecraft sterilization, quarantine standards, and policies is provided. The specific contamination probabilities of the U.S.S.R. Venus 3 flyby are included.

Werber, M.↗

Determination of failure limits for sterilizable solid rocket motor

A structural evaluation to establish probable failure limits and a series of environmental tests involving temperature cycling, sustained acceleration, and vibration were conducted on an 18-inch diameter solid rocket motor. Despite the fact that thermal, acceleration and vibration loads representing a severe overtest of conventional environmental requirements were imposed on the sterilizable motor, no structural failure of the grain or flexible support system was detected. The following significant conclusions are considered justified. It is concluded that: (1) the flexible grain retention system, which permitted heat sterilization at 275 F on the test motor, can readily be adopted to meet the environmental requirements of an operational motor design, and (2) if further substantiation of structural integrity is desired, the motor used is considered acceptable for static firing.

Lambert, W. L.↗

Summary of the proceedings of the Mars Surface Sample Return Symposium

A summary is made of various technical and scientific aspects of a Mars surface sample return mission. Particular attention was focused on the question of back contamination. Data are also given on problems inherent in the back contamination issue and return sample mission; areas where additional research is needed were pointed out. Quarantine procedures, safety measures, and sterilization effects on organic-inorganic data, and biological problems were also dealt with.

Devincenzi, D. L.↗

Microbial metabolism and dynamic changes in the electrical conductivity of soil solutions - A method for detecting extraterrestrial life

Experiments are reported which show that measuring metabolic activity in soil solutions by means of dynamic changes in electrical conductivity, water-soluble Ca, or water-soluble Mg is a feasible life detection method. The addition of 0.5% glucose solutions to 12 different air-dried soils always resulted in increases in all three of these parameters. The kinetics and magnitude of these changes for at least two and usually all three of the parameters over a 14-day period were clearly distinguishable from the changes in heat-sterilized controls or unsterilized controls without added glucose. In general, maximal values were achieved more rapidly under aerobic than under anaerobic incubation.

Silverman, M. P.↗

Computer simulation of thermal and fluid systems for MIUS integration and subsystems test /MIST/ laboratory

This paper describes the application of the SINDA (systems improved numerical differencing analyzer) computer program to simulate the operation of the NASA/JSC MIUS integration and subsystems test (MIST) laboratory. The MIST laboratory is designed to test the integration capability of the following subsystems of a modular integrated utility system (MIUS): (1) electric power generation, (2) space heating and cooling, (3) solid waste disposal, (4) potable water supply, and (5) waste water treatment. The SINDA/MIST computer model is designed to simulate the response of these subsystems to externally impressed loads. The computer model determines the amount of recovered waste heat from the prime mover exhaust, water jacket and oil/aftercooler and from the incinerator. This recovered waste heat is used in the model to heat potable water, for space heating, absorption air conditioning, waste water sterilization, and to provide for thermal storage. The details of the thermal and fluid simulation of MIST including the system configuration, modes of operation modeled, SINDA model characteristics and the results of several analyses are described.

Rochelle, W. C.↗

Ecology and thermal inactivation of microbes in and on interplanetary space vehicle components

Dry heat treatment is specified as the preferred means for the terminal sterilization of spacecraft and for decontamination of spacecraft components. The presence of organisms highly resistant to dry heat in soil and fallout around assembly and industrial manufacturing areas is shown. The dry heat survival characteristics of the Cape Kennedy isolate 4-6 B. brevis spores is demonstrated. The presence of hardy organisms from soil samples obtained from geographical areas of the United States is shown. A resistant fraction appears to occur in low numbers in a soil sample. The heat resistance characteristics of 4-6 B. brevis and B. subtilis var. niger spores are compared. Their morphological characteristics are compared by scanning electron microscopy.

Reyes, A. L.↗

Application and utilization of a space chamber for the drying and decontamination of books, documents and other materials

Truckloads of materials such as rare books, papers, engineering drawings, blue prints, art work, leather objects such as shoes, and clothing were successfully dried, decontaminated and impregnated against future infestation by microorganisms in a large 12 x 24 foot vacuum chamber designed originally for testing unmanned spacecraft. The process is unique in that it allows either frozen or wet material, soaked by some castastrophic event to be dried and sterilized in the same chamber with a minimum of handling and transportation.

Koesterer, M. G.↗

The Screwworm Eradication Data System (SEDS)

One of the objectives of the SEDS was to build a versatile, yet economical, interactive system on the existing digital PDP 11/45 computers used in the Skylab production processing system. Previous experience on various interactive systems gave some background in the best methods of handling image data, registering and correcting the data, and extracting useful information from the images. The screwworm was eliminated in many parts of the United States by air-dropping sterile screwworms in areas where screwworms breed. The reproduction of the screwworm can be more or less predicted based on weather conditions -- cold weather slows the breeding, warm weather fosters it, and some moisture appears to enhance breeding conditions which, in turn, improves the chances of screwworm population growth. To obtain temperature and moisture conditions from remote areas, the NOAA improved TIROS operational satellite was selected to give data from the very high resolution radiometer (VHRR). The VHRR is a two-channel scanning instrument sensitive to energy in the visible spectrum from 0.6 to 0.7 micrometers and the infrared spectrum from 10.5 to 12.5 micrometers.

Quinn, M. J.↗

Studies related to the development of the Viking 1975 labeled release experiment

The labeled release life detection experiment on the Viking 1975 Mars mission is based on the concept that microorganisms will metabolize radioactive organic substrates in a nutrient medium and release radioactive carbon dioxide. Several experiments, using laboratory equipment, were carried out to evaluate various aspects of the concept. Results indicate: (1) label is released by sterilization-treated soil, (2) substantial quantities of label are retained in solution under basic conditions, (3) the substrate used, as well as position of label in the molecule, affect release of label, (4) label release is depressed by radiolytic decomposition of substrates, and (5) About 100,000 organisms are required to produce a detectable response. These results, suggest additional areas for testing, add to the data base for interpretation of flight results, and have significance for broader application of this technique for assessing microbial activity.

Devincenzi, D. L.↗

Health protection and food preservation by gamma irradiation

Results of several major studies on food systems for space missions beginning with Apollo 12 through Apollo-Soyuz and investigations of the application of irradiation to food for manned space flight are reported. The study of flight food systems involved the application of radurization (pasteurizing levels) doses of gamma irradiation to flour and bread supplied by Pepperidge Farms in advance of the missions. All flights from Apollo 12 through 17 carried irradiated fresh bread. On Apollo 17, cooperation with Natick Laboratories permitted the introduction of a ham sandwich using irradiated bread and irradiated sterile ham. Investigations centered on irradiated bread were conducted during the course of these missions. Studies were applied to the concept of improving fresh bread from the point of view of mold inhibition. The studies considered how irradiation could best be applied at what levels and on a variety of bread types. Throughout the studies of the application of gamma irradiation the emphasis was placed upon using low levels of irradiation in the pasteurizing or radurizing doses--under a Megarad. The primary goal was to determine if a public health benefit could be demonstrated using radurization along with food preservation and food quality improvements. The public health benefit would be parallel to that of pasteurization of milk as a concept. Publications are included providing the details of these observations, one dealing with the flour characteristics and the other dealing with the influence on fresh bread types. These demonstrate the major findings noted during the period of the studies examining bread.

Source record↗

The Evolution of the Viking Landing Gear

The primary function of the landing gear is to dissipate touchdown energy in a controlled fashion that minimizes the landing shock for onboard components while maximizing ground clearance and the probability of landing stability over the range of possible surface and touchdown parameter variations. Many other mission requirements and constraints were translated into the evolving Viking landing gear design as they arose. Hence, design considerations included such factors as prelaunch heat sterilization and noncontamination of the Martian landing site, gear stowage and deployment, terminal descent engine shutdown initiation, structural load attenuation, hard/soft landing surface capability, reliability, weight, and post-landed stability.

Pohlen, J. C.↗

Detailed results of ASTP experiment MA-011

This experiment was developed in order to conduct engineering and operational tests of electrokinetic equipment in a micro-gravity environment. The experimental hardware in general functioned as planned and electrophoretic separations were obtained in space. The results indicated the development of satisfactory sample collection, return, and preservation techniques. The application of a near-zero zeta potential interior wall coating to the experimental columns, confirmation of biocompatibility of all appropriate hardware components, and use of a sterile operating environment provided a significant step forward in the development of a biological processing facility in space. A separation of a test of aldehyde-fixed rabbit, human, and horse red blood cells was obtained. Human kidney cells were separated into several components and viable cells returned to earth. The isotachophoretic separation of red cells was also demonstrated. Problems associated with the hardware led to a lack of success in the attempt to separate subpopulations of human lymphocytes.

Seaman, G. V. F.↗