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This volume, the first of two volumes, is a collection of 29 unclassified/unlimited-distribution papers which were presented at the 50th Joint Army-Navy-NASA-Air Force (JANNAF) Propulsion Meeting, held 11-13 July 2001 at the Salt Lake City Marriott Hotel in Salt Lake City, Utah.
Observations from the Voyager and Galileo spacecraft have revealed Jupiter's moon Io to be the most volcanically active body of our Solar System. The Galileo Near Infrared Imaging Spectrometer (NIMS) detected extensive deposits of sulfur compounds, elemental sulfur and SO2 frost on the surface of Io. There are extreme temperature variations on Io's surface, ranging from -130 C to over 2000 C at the Pillan Patera volcanic vent. The active volcanoes, fumaroles, calderas, and lava lakes and vast sulfur deposits on this frozen moon indicate that analogs of sulfur- and sulfate-reducing bacteria might inhabit Io. Hence Io may have great significance to Astrobiology. Earth's life forms that depend on sulfur respiration are members of two domains: Bacteria and Archaea. Two basic links of the biogeochemical sulfur cycle of Earth have been studied: 1) the sulfur oxidizing process (occurring at aerobic conditions) and 2) the process of sulfur-reduction to hydrogen sulfide (anaerobic conditions). Sulfate-reducing bacteria (StRB) and sulfur-reducing bacteria (SrRB) are responsible for anaerobic reducing processes. At the present time the systematics of StRB include over 112 species distributed into 35 genera of Bacteria and Archaea. Moderately thermophilic and mesophilic SrRB belong to the Bacteria. The hyperthermophilic SrRB predominately belong to the domain Archaea and are included in the genera: Pyrodictium, Thermoproteus, Pyrobaculum, Thermophilum, Desulfurococcus, and Thermodiscus. The StRB and SrRB use a wide spectrum of substrates as electron donors for lithotrophic and heterotrophic type nutrition. The electron acceptors for the StRB include: sulfate, thiosulfate, sulfite, sulfur, arsenate, dithionite, tetrathionate, sulfur monoxide, iron, nitrite, selenite, fumarate, oxygen, carbon dioxide, and chlorine-containing phenol compounds. The Sulfate- and Sulfur-reducing bacteria are widely distributed in anaerobic ecosystems, including extreme environments like hot springs, deepsea hydrothermal vents, soda and high salinity lakes, and cryo-environments. Furthermore, the StRB and SrRB have Astrobiological significance as these anaerobic extremophiles may represent the dominant relic life forms that inhabited our planet during the extensive volcanic activity in the Earth's early evolutionary period.
The NASA Airborne Visible/Infrared Imaging Spectrometer (AVIRIS) measures spectra from 370 to 2500 nm with nominally 10-nm sampling and resolution. The spectra are acquired as images with an 11 km width and up to 800 km length from the ER-2 platform or 2.1 km width and 160 km length from the Twin Otter platform. AVIRIS measurements are used for a range of Earth science research and application objectives. The molecular absorption and particle scattering properties expressed in the calibrated AVIRIS measurements are used. For both science research and application objectives, calibration of the AVIRIS spectra is required to produce useful results. Each year prior to the flight season AVIRIS is calibrated in the laboratory. However, the temperature, pressure, vibration, and observational geometry, as well as mechanical, electrical, and operational interfaces of the laboratory are different than the environment on board the airborne platform. To validate the calibration of AVIRIS in the flight environment, an inflight calibration experiment is orchestrated at the beginning of each flight season. In most years additional inflight calibration experiments occur towards the middle and end of the flight season as well. For an inflight calibration experiment, AVIRIS acquires airborne data over a designated calibration target. In concert with the airborne data acquisition, surface and atmospheric properties at the calibration target are measured in situ. These in situ measurements are used to constrain a radiative transfer code and predict the radiance incident at the AVIRIS instrument from the calibration target. This prediction is compared with the AVIRIS-measured radiance to validate the calibration of AVIRIS in the flight environment. Additional properties (such as the AVIRIS inflight measurement precision) are determined as well. This paper presents measurements, analyses, and results from the inflight calibration experiment held on the dry lake bed surface of Rogers Dry Lake, California, on June 5, 2000.
Extremophiles are microorganisms that thrive in, from an anthropocentric view, extreme environments including hot springs, soda lakes and arctic water. This ability of survival at extreme conditions has rendered extremophiles to be of interest in astrobiology, evolutionary biology as well as in industrial applications. Of particular interest to the biotechnology industry are the biological catalysts of the extremophiles, the extremozymes, whose unique stabilities at extreme conditions make them potential sources of novel enzymes in industrial applications. There are two major approaches to microbial enzyme production. This entails enzyme isolation directly from the natural host or creating a recombinant expression system whereby the targeted enzyme can be overexpressed in a mesophilic host. We are employing both methods in the effort to produce alpha-amylases from a hyperthermophilic archaeon (Thermococcus) isolated from a hydrothermal vent in the Atlantic Ocean, as well as from alkaliphilic bacteria (Bacillus) isolated from a soda lake in Tanzania. Alpha-amylases catalyze the hydrolysis of internal alpha-1,4-glycosidic linkages in starch to produce smaller sugars. Thermostable alpha-amylases are used in the liquefaction of starch for production of fructose and glucose syrups, whereas alpha-amylases stable at high pH have potential as detergent additives. The alpha-amylase encoding gene from Thermococcus was PCR amplified using carefully designed primers and analyzed using bioinformatics tools such as BLAST and Multiple Sequence Alignment for cloning and expression in E.coli. Four strains of Bacillus were grown in alkaline starch-enriched medium of which the culture supernatant was used as enzyme source. Amylolytic activity was detected using the starch-iodine method.
Terrestrial biotas from microbially dominated hypersaline environments will help us understand microbial fossilization processes. Hypersaline tolerant biota from Storr's Lake, San Salvador Island (Bahamas), Mono Lake (California), and the Dead Sea (Israel) represent marine and nonmarine sites for comparative studies of potential analogs for interpreting some Mars meteorites and Mars sample return rocks [1,2,3,4,5,6]. The purpose of this study is to compare microbial fossilization processes, the dominant associated minerals, and potential diagenic implications.
Since the two Voyagers passed by Jupiter in 1979, it has been known that volcanic activity is ubiquitous on the surface of Io. With over 400 volcanic centers, Io is even more volcanically active than the earth with massive flood basalt-style eruptions and komatitite lavas a common occurrence. Additionally, some volcanoes appear to be giant lava lakes, with violent activity churning the crust of the lake for periods of 20 years or more. Finally, sulfur is believed to play a large role in Io's volcanism, be it as a primary lava or as a secondary product of large, high-temperature eruptions. By studying one volcano in particular, Gish Bar Patera, one can observe many of these characteristics in one volcanic center.
At least some meteoritic nanodiamonds are likely of presolar origin because of their association with anomalous Xe-HL and Te isotopic components indicative of a supernova (SN) origin. But the abundance of Xe is such that only approx. 1 in 10(exp 6) nano-diamonds contains a Xe atom, and the bulk C-13/C-12 composition of nano-diamond acid residues is chondritic (solar). Therefore, it is possible that a significant fraction of meteoritic nano-diamonds formed within the solar nebula. Nano-diamonds have recently been detected for the first time within the accretion discs of young stars by the Infrared Space Observatory (ISO). No comparable evidence of nanodiamonds in the interstellar medium has yet been found. We have identified nano-diamonds in acid etched thin-sections of meteorites, polar micrometeorites, and interplanetary dust particles (IDPs) with the goal of determining their distribution as a function of heliocentric distance. (It is assumed the meteorites and the polar micrometeorites are from asteroids at 2-4 AU and at least some of the IDPs are from comets at >50AU). We found that nano-diamonds are heterogeneously distributed throughout carbon-rich meteoritic materials (we identified them in some IDPs and not in others), and that their abundance may actually decrease with heliocentric distance, consistent with the hypothesis that some of them formed within the inner solar system and not in a presolar (SN) environment. In order to gain further insight about the origins of meteoritic nano-diamonds we are currently investigating their distribution in unetched thin-sections. We have examined a chondritic cluster IDP (U220GCA), fragments of the Tagish Lake (CM1) meteorite, and a SN graphite spherule (KE3d8) isolated from the Murchison (CM) meteorite. We selected U220GCA because its nano-diamond abundance (in acid etched thin-sections) appears to be as much as approx. 10X higher than in Murchison matrix, Tagish Lake because it has a higher reported nano-diamond abundance than other carbonaceous chondrites (approx. 3650-4330 ppm), and KE3d8 because it is a carbon spherule with an isotopic composition suggesting that it is a bone fide presolar SN grain.
While Loki is the most active volcanic center on Io, major questions remain concerning the nature of that activity. Rathbun et al. showed that the activity was semi-periodic, and suggested it was due to a resurfacing wave which swept across a lava lake as the crust cooled and become unstable. However in 2001 new observations showed that an intermediate level, less periodic mode of activity had apparently begun. Galileo-NIMS observations of Loki clearly show that the highest temperatures are found near the edge of the patera, consistent with disruption of a lava lake at the margins. NIMS observations also show gradients in temperature across the patera which, when modeled in terms of lava cooling models, are generally consistent with ages expected for the resurfacing wave but may also be consistent with spreading flows. We present a further analysis of NIMS data from I24 and I32 which help define the nature of the temperature variations present in Loki patera, along with Galileo-SSI images from the G1-I32 flybys which show albedo changes apparently correlated with the "periodic" activity measured from ground-based observations.
The P-type asteroids, together with the D asteroids, had been believed to be one of the most primitive asteroid classes having surface materials rich in carbon and/or organics. Upon a fall of a new type of meteorite, Tagish Lake in 2000, we came to have a possible sample of the D (and/or T) asteroids. In both spectrally and distance from the sun, the P asteroids are located in between the C/G/B/F asteroids and the D asteroids. Because it is believed that the former group are similar to (thermally metamorphosed) CI/CM chondrites and the latter the Tagish Lake meteorite, the surface material of the P asteroids may be understood in combination of those two meteorite groups. Taking that direction, this paper presents possibly the first quantitative characterization of the P asteroids in terms of carbonaceous chondrites and their experimental derivatives.
The generation of waves by winds across Earth's water oceans is a topic of enduring fascination. However, the physics of the problem are rather forbidding and thus the relationships between real-world windspeed and sea state tend to be empirical. Such empirical relations are of limited utility in environments where the physical parameters are different, such as the surfaces of other planets. These environments have only recently come to oceanographers attention, with the discovery of ancient shorelines and lakes on Mars, and the prospects for and recent evidence of lakes and seas of liquid hydrocarbons on Saturn's moon Titan. We are aware of only one other published experimental wind-water tunnel study where the fluid parameters have been varied. This used artificially-generated mm-scale waves at 3.8-7.6 Hz in water, glycerol solutions (higher viscosity) and surfactant solutions (lower surface tension). Lower viscosity solutions had higher wave growth rates: surprisingly, higher surface tension led to more rapid wave growth. The liquid density was not appreciably varied, and 1 bar air was used throughout.We used the MARSWIT (Mars Wind Tunnel) operated by ASU at NASA Ames. A fiberglass tray (5 cm x 120 cm x 75 cm) was installed in the tunnel, with an approx. 1:5 ramp to prevent strong flow separation. The tray was filled to a depth of about 4 cm. Sensors were clamped to the tray itself or held by a steel and aluminium frame just above the water level. A towel was draped on the water surface at the downwind end of the tray to act as a damper to suppress wave reflection. Position-sensitive infrared (IR) reflection sensors (Sharp GP12D02) and ultrasonic rangers (Devantech DF-04) used in mobile robotics were used as water level sensors. The tray was observed with a video camera, whose output could be viewed on a monitor and recorded on VHS tape.
Hydrogen isotopes are highly variable among primitive meteorites and interplanetary dust particles (IDPs) [1, 2]. In particular, many primitive objects exhibit D (and N-15) enrichments, relative to terrestrial values, thought to represent partial preservation of presolar material fractionated in molecular clouds. However, the diversity of D/H ratios among IDPs and chondrites indicates a complex history of processing in the solar nebula and on meteorite parent bodies. Deconvolving this record requires identification and characterization of the carriers of D enrichments in different objects. Isotopic imaging has proven to be a powerful method to quantitatively explore the distribution of D/H ratios on a one to several m scale in IDPs [2-4] and the CR chondrite Renazzo [5, 6]. In this study, we have used ion imaging to explore the microscale D/H distribution of two carbonaceous chondrites, Tagish Lake (unique) and Al Rais (CR2). Previous D/H measurements (on a ~tens of microns scale) of Tagish Lake matrix fragments by Messenger [7] and Engrand et al. [8] have found different results, most likely related to the analytical techniques used. Previous work has also shown a large range of D/H ratios in CR chondrites, including very large variations on a scale of a few microns [5, 6, 9].
The presentations in this session are: 1. A Prototype Life Detection Chip 2. The Geology of Atlantis Basin, Mars, and Its Astrobiological Interest 3. Collecting Bacteria Together with Aerosols in the Martian Atmosphere by the FOELDIX Experimental Instrument Developed with a Nutrient Detector Pattern: Model Measurements of Effectivity 4. 2D and 3D X-ray Imaging of Microorganisms in Meteorites Using Complexity Analysis to Distinguish Field Images of Stromatoloids from Surrounding Rock Matrix in 3.45 Ga Strelley Pool Chert, Western Australia 4. Characterization of Two Isolates from Andean Lakes in Bolivia Short Time Scale Evolution of Microbiolites in Rapidly Receding Altiplanic Lakes: Learning How to Recognize Changing Signatures of Life 5. The Effect of Salts on Electrospray Ionization of Amino Acids in the Negative Mode 6. Determination of Aromatic Ring Number Using Multi-Channel Deep UV Native Fluorescence 7. Microbial D/H Fractionation in Extraterrestrial Materials: Application to Micrometeorites and Mars 8. Carbon Isotope Characteristics of Spring-fed Iron-precipitating Microbial Mats 9. Amino Acid Survival Under Ambient Martian Surface UV Lighting Extraction of Organic Molecules from Terrestrial Material: Quantitative Yields from Heat and Water Extractions 10. Laboratory Detection and Analysis of Organic Compounds in Rocks Using HPLC and XRD Methods 11. Thermal Decomposition of Siderite-Pyrite Assemblages: Implications for Sulfide Mineralogy in Martian Meteorite ALH84001 Carbonate Globules 12. Determination of the Three-Dimensional Morphology of ALH84001 and Biogenic MV-1 Magnetite: Comparison of Results from Electron Tomography and Classical Transmission Electron Microscopy 13. On the Possibility of a Crypto-Biotic Crust on Mars Based on Northern and Southern Ringed Polar Dune Spots 14. Comparative Planetology of the Terrestrial Inner Planets: Implications for Astrobiology 15. A Possible Europa Exobiology 16. A Possible Biogeochemical Model for Titan
Contents include the following: 1. Characterization of Tagish Lake organic content. The first two grant years were largely devoted to the molecular and isotopic analyses of Tagish Lake organic composition. This carbonaceous meteorite fell in Canada in the winter of the year 2000, and its exceptional atmospheric entry and subsequent recovery (e. g., the sample was recovered and stored by avoiding hand contact and above freezing temperatures) contributed in providing a rare and pristine extraterrestrial material. 2. Chiral analyses of Murchison and Murray soluble organics. One of the most intriguing finding in regard to soluble meteorite organics is the presence within the amino acid suite of some compounds displaying L-enantiomeric excesses. This configuration is exclusive in the amino acids of terrestrial proteins and the finding has raised speculations of a possible role of amino acids from meteorites in the origin of homochirality on the early Earth. The main objective for this NASA funding was the characterization of enantiomeric excesses in meteorites and we have conducted several studies toward establishing their distribution and indignity.
Evidence for the presence of ice and fluids near the surface of Mars in both the distant and recent past is growing with each new mission to the Planet. One explanation for fluids forming springlike features on Mars is the discharge of subsurface brines. Brines offer potential refugia for extant Martian life, and near surface ice could preserve a record of past life on the planet. Proven techniques to get underground to sample these environments, and get below the disruptive influence of the surface oxidant and radiation regime, will be critical for future astrobiology missions to Mars. Our Astrobiology for Science and Technology for Exploring Planets (ASTEP) project has the goal to develop and test a novel ultrasonic corer in a Mars analog environment, the McMurdo Dry valleys, Antarctica, and to detect and describe life in a previously unstudied extreme ecosystem; Lake Vida (Fig. 1), an ice-sealed lake.
Diverse evidence shows a direct correlation between episodic endogenetic events of the Tharsis magmatic complex (TMC)/Superplume, flood inundations in the northern plains, and glacial/ lacustrine/ice sheet activity in the south polar region, which includes Hellas and Argyre impact basins, corroborating the MEGAOUTFLO hypothesis. The TMC encompasses a total surface area of approximately 2 x 10(exp 7) sq km, which is slightly larger than the estimated size of the Southern Pacific Superplume. These hydrologic events include: (1) a Noachian to possibly Early Hesperian oceanic epoch and related atmospheric and environmental change (a water body covering about 1/3 of the planet s surface area) related to the incipient development of Tharsis Superplume and the northwestern sloping valleys (NSVs) and possibly early circum-Chryse development, the northwest and northeast watersheds of Tharsis, respectively, (2) a smaller ocean inset within the former larger ocean related to extensive Late Hesperian to Early Amazonian effusive volcanism at Tharsis and Elysium and incisement of the circum-Chryse outflow system. During this time, magmatic/plume-driven tectonic activity transitioned into more centralized volcanism. This Late Hesperian water body may have simply diminished into smaller seas and/or lakes during the Amazonian Period, or renewed activity at Tharsis and Elysium resulted in brief perturbations from the prevailing cold and dry climatic conditions to later form minor seas or lakes. All of the hydrologic phases transitioned into extensive periods of quiescence.
The carbon isotope effects associated with synthesis of methane from acetate have been determined for Methanosarcina barkeri 227 and for methanogenic archaea in sediments of Wintergreen Lake, Michigan. At 37 degrees C, the 13C isotope effect for the reaction acetate (methyl carbon) --> methane, as measured in replicate experiments with M. barkeri, was - 21.3% +/- 0.3%. The isotope effect at the carboxyl portion of acetate was essentially equal, indicating participation of both positions in the rate-determining step, as expected for reactions catalyzed by carbon monoxide dehydrogenase. A similar isotope effect, - 19.2% +/- 0.3% was found for this reaction in the natural community (temperature = 20 degrees C). Given these observations, it has been possible to model the flow of carbon to methane within lake sediment communities and to account for carbon isotope compositions of evolving methane. Extension of the model allows interpretation of seasonal fluctuations in 13C contents of methane in other systems.
The fungal microflora of a dry valley in Southern Victoria Land near McMurdo Sound, Antarctica, was investigated. Samples were collected from introduced objects such as a mummified penguin and spent chewing tobacco in addition to the sparse soil found in rock fissures, isolated moss colonies, shoreline deposit materials, CaCO3 precipitates, and microbial mat debris obtained from the frozen surface of the lake in the basin of Taylor Valley. Using conventional media and techniques, all collection sites yielded populations of yeasts and filamentous fungi. Water samples and live microbial mats from beneath the lake ice yielded species of fungi along with an abundance of bacteria.
A key objective of the Cirrus Regional Study of Tropical Anvils and Cirrus Layers - Florida Area Cirrus Experiment (CRYSTAL-FACE) is to understand the relationships between properties of tropical convective cloud systems and the lifecycle of the extended cirrus anvils they produce. We report here on a case study of 23 July 2002 where a line of land-based convective storms was generated between Lake Okeechobee and the Florida east coast as a result of complex interactions between lake and sea breeze fronts and outflow boundaries. A central goal of this study is to develop a description of convective input to the anvil system and to quantify the ongoing dynamical forcing of anvil processes by mesoscale and large-scale dynamics. This information is then used to force high-resolution cloud simulations with a model that explicitly resolves cloud microphysical processes (bin model) for study of cirrus anvil microphysical development.