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At least 1,477 records · Page 82

Aberrant FBXW7-mediated ubiquitination and degradation of ZMYND8 enhances tumor progression and stemness in bladder cancer

ZMYND8, an epigenetic regulator, was identified as a common oncogene across various tumors. However, little was reported about the association between ZMYND8 and bladder cancer. Besides, aberrant mechanisms that contribute to abnormal ZMYND8 expressions still remain unclear. In the current study, we first found that ZMYND8 protein levels were significantly elevated in Bca samples versus normal tissues, but not the mRNA levels. We then utilized the Cell Counting Kit-8 (CCK-8) assay, clone formation assay and transwell analysis to confirm that ZMYND8 could remarkably promote the tumor progression in vitro, including growth capacity and migration. Bioinformatic predictive analysis revealed that E3 ubiquitin ligase FBXW7 interacts directly with ZMYND8 and degrades ZMYND8 in a polyubiquitination manner. Low FBXW7 was a hazard factor for promoting and depending on accumulated ZMYND8 proteins to promote Bca progression. Gene set enrichment analysis (GSEA) further indicated that ZMYND8 was notably associated with stemness process, which was well functionally validated. Lastly, ZMYND8 deficiency was observed to inhibit tumor growth of Bca in vivo, revealing a promising translational significance in Bca treatment. In conclusion, our study for the first time provided evidence for a novel mechanism of FBXW7/ZMYND8 axis in Bca, providing therapeutic vulnerability for individualized cancer treatment.

60 APPLIED LIFE SCIENCES↗

PKA regulatory subunit Bcy1 couples growth, lipid metabolism, and fermentation during anaerobic xylose growth in Saccharomyces cerevisiae

Organisms have evolved elaborate physiological pathways that regulate growth, proliferation, metabolism, and stress response. These pathways must be properly coordinated to elicit the appropriate response to an ever-changing environment. While individual pathways have been well studied in a variety of model systems, there remains much to uncover about how pathways are integrated to produce systemic changes in a cell, especially in dynamic conditions. We previously showed that deletion of Protein Kinase A (PKA) regulatory subunit BCY1 can decouple growth and metabolism in Saccharomyces cerevisiae engineered for anaerobic xylose fermentation, allowing for robust fermentation in the absence of division. This provides an opportunity to understand how PKA signaling normally coordinates these processes. Here, we integrated transcriptomic, lipidomic, and phospho-proteomic responses upon a glucose to xylose shift across a series of strains with different genetic mutations promoting either coupled or decoupled xylose-dependent growth and metabolism. Together, results suggested that defects in lipid homeostasis limit growth in the bcy1Δ strain despite robust metabolism. To further understand this mechanism, we performed adaptive laboratory evolutions to re-evolve coupled growth and metabolism in the bcy1Δ parental strain. The evolved strain harbored mutations in PKA subunit TPK1 and lipid regulator OPI1 , among other genes, and evolved changes in lipid profiles and gene expression. Deletion of the evolved opi1 gene partially reverted the strain’s phenotype to the bcy1Δ parent, with reduced growth and robust xylose fermentation. We suggest several models for how cells coordinate growth, metabolism, and other responses in budding yeast and how restructuring these processes enables anaerobic xylose utilization.

59 BASIC BIOLOGICAL SCIENCES↗

Multi-Input Regulation and Logic with T7 Promoters in Cells and Cell-Free Systems

Engineered gene circuits offer an opportunity to harness biological systems for biotechnological and biomedical applications. However, reliance on native host promoters for the construction of circuit elements, such as logic gates, can make the implementation of predictable, independently functioning circuits difficult. In contrast, T7 promoters offer a simple orthogonal expression system for use in a variety of cellular backgrounds and even in cell-free systems. Here we develop a T7 promoter system that can be regulated by two different transcriptional repressors for the construction of a logic gate that functions in cells and in cell-free systems. We first present LacI repressible T7lacO promoters that are regulated from a distal lac operator site for repression. We next explore the positioning of a tet operator site within the T7lacO framework to create T7 promoters that respond to tet and lac repressors and realize an IMPLIES gate. Finally, we demonstrate that these dual input sensitive promoters function in an E. coli cellfree protein expression system. Our results expand the utility of T7 promoters in cell based as well as cell-free synthetic biology applications.

59 BASIC BIOLOGICAL SCIENCES↗

Aerodynamic Heat-Power Engine Operating on a Closed Cycle

Hot-air engines with dynamic compressors and turbines offer new prospects of success through utilization of units of high efficiencies and through the employment of modern materials of great strength at high temperature. Particular consideration is given to an aerodynamic prime mover operating on a closed circuit and heated externally. Increase of the pressure level of the circulating air permits a great increase of limit load of the unit. This also affords a possibility of regulation for which the internal efficiency of the unit changes but slightly. The effect of pressure and temperature losses is investigated. A general discussion is given of the experimental installation operating at the Escher Wyss plant in Zurich for a considerable time at high temperatures.

Ackeret, J.↗

Joint Test Plan for Gas Dynamic Spray Technology Demonstration

Air Force Space Command (AFSPC) and NASA have similar missions, facilities, and structures located in similar harsh environments. Both are responsible for a number of facilities/structures with metallic structural and non-structural components in highly and moderately corrosive environments. Regardless of the corrosivity of the environment, all metals require periodic maintenance activity to guard against the insidious effects of corrosion and thus ensure that structures meet or exceed design or performance life. The standard practice for protecting metallic substrates in atmospheric environments is the use of an applied coating system. Current coating systems used across AFSPC and NASA contain volatile organic compounds (VOCs) and hazardous air pollutants (HAPs). These coatings are subject to environmental regulations at the Federal and State levels that limit their usage. In addition, these coatings often cannot withstand the high temperatures and exhaust that may be experienced by AFSPC and NASA structures. In response to these concerns, AFSPC and NASA have approved the use of thermal spray coatings (TSCs). Thermal spray coatings are extremely durable and environmentally friendly coating alternatives, but utilize large cumbersome equipment for application that make the coatings difficult and time consuming to repair. Other concerns include difficulties coating complex geometries and the cost of equipment, training, and materials. Gas Dynamic Spray (GDS) technology (also known as Cold Spray) will be evaluated as a smaller, more maneuverable repair method as well as for areas where thermal spray techniques are not as effective. The technology can result in reduced maintenance and thus reduced hazardous materials/wastes associated with current processes. Thermal spray and GDS coatings also have no VOCs and are environmentally preferable coatings. To achieve a condition suitable for the application of a coating system, including GDS coatings, the substrate must undergo some type of surface preparation and/or depainting operation to ensure adhesion of the new coating system. The GDS unit selected for demonstration has a powder feeding system that can be used for surface preparation or coating application. The surface preparation feature will also be examined. The primary objective of this effort is to demonstrate GDS technology as a repair method for TSCs. The project will also determine the optimal GDS coating thickness for acceptable performance. Successful completion of this project will result in approval of GDS technology as a repair method for TSCs at AFSPC and NASA installations and will improve corrosion protection at critical systems, facilitate easier maintenance activity, extend maintenance cycles, eliminate flight hardware contamination, and reduce the amount of hazardous waste generated.

Lewis, Pattie↗

State of the Art in Large-Scale Soil Moisture Monitoring

Soil moisture is an essential climate variable influencing land atmosphere interactions, an essential hydrologic variable impacting rainfall runoff processes, an essential ecological variable regulating net ecosystem exchange, and an essential agricultural variable constraining food security. Large-scale soil moisture monitoring has advanced in recent years creating opportunities to transform scientific understanding of soil moisture and related processes. These advances are being driven by researchers from a broad range of disciplines, but this complicates collaboration and communication. For some applications, the science required to utilize large-scale soil moisture data is poorly developed. In this review, we describe the state of the art in large-scale soil moisture monitoring and identify some critical needs for research to optimize the use of increasingly available soil moisture data. We review representative examples of 1) emerging in situ and proximal sensing techniques, 2) dedicated soil moisture remote sensing missions, 3) soil moisture monitoring networks, and 4) applications of large-scale soil moisture measurements. Significant near-term progress seems possible in the use of large-scale soil moisture data for drought monitoring. Assimilation of soil moisture data for meteorological or hydrologic forecasting also shows promise, but significant challenges related to model structures and model errors remain. Little progress has been made yet in the use of large-scale soil moisture observations within the context of ecological or agricultural modeling. Opportunities abound to advance the science and practice of large-scale soil moisture monitoring for the sake of improved Earth system monitoring, modeling, and forecasting.

Review↗

Design of a GRCop-42 Regeneratively Cooled Thrust Chamber Assembly and Feed System

An additively manufactured thrust chamber assembly was designed and printed using laser powder bed fusion. The NASA-developed material GRCop-42 was used for its high strength and high temperature characteristics. The combustion chamber and nozzle utilize regenerative coolant channels to achieve long duration hot fire tests. Design for additive manufacturing was used to create geometries not easily attainable with traditional machining. The thrust chamber assembly was post processed to ensure print quality and verify for testing. A feed system – encompassing all propellant lines, valves, sensors, and tanks – was designed and built to enable steady state, pressure regulated testing. Pressure transducers, thermocouples, and load cells were placed to enable measurements of the propellant properties and engine performance. Venturis and orifices were used throughout the system to control the flow rates to the thrust chamber. Aside from providing propellants, the feed system provides pressure using nitrogen, and purges the lines during shutdown. The design process for this testing platform is described. A testing campaign on the hardware will be conducted by the Akronauts Rocket Design Team at the University of Akron in Spring 2023.

Dillon M. Petty↗

Design of a GRCop-42 Regeneratively Cooled Thrust Chamber Assembly and Feed System

An additively manufactured thrust chamber assembly was designed and printed using laser powder bed fusion. The NASA-developed material GRCop-42 was used for its high strength and high temperature characteristics. The combustion chamber and nozzle utilize regenerative coolant channels to achieve long duration hot fire tests. Design for additive manufacturing was used to create geometries not easily attainable with traditional machining. The thrust chamber assembly was post processed to ensure print quality and verify for testing. A feed system – encompassing all propellant lines, valves, sensors, and tanks – was designed and built to enable steady state, pressure regulated testing. Pressure transducers, thermocouples, and load cells were placed to enable measurements of the propellant properties and engine performance. Venturis and orifices were used throughout the system to control the flow rates to the thrust chamber. Aside from providing propellants, the feed system provides pressure using nitrogen, and purges the lines during shutdown. The design process for this testing platform is described. A testing campaign on the hardware will be conducted by the Akronauts Rocket Design Team at the University of Akron in Spring 2023.

Dillon M. Petty↗

Damaged Fuel in the United States

Over the years of commercial nuclear power plant operations in the United States (U.S.), many fuel assemblies have lost the capability to perform all of their desired functions. These assemblies have lost the capability to be handled, stored, or transported to meet regulations resulting in them being classified as damaged fuel. The causes of these failed assemblies are diverse and plant-specific. The majority of these failed assemblies are contained in a damaged fuel can that will be used in conjunction with a storage and/or transportation system. The storage and transportation systems have a limit on how many slots can be filled with damaged fuel cans. A damaged fuel can is generally a stainless steel container that confines damaged SNF and is closed on its end by screened openings. These screened openings allow gaseous and liquid media to escape but minimize the dispersal of gross particulate material. Out of an abundance of caution a few reactors have loaded high burnup fuel into damaged fuel cans. Damaged fuel is not licensed for storage or transport in the U.S., because the regulations for storage and transport do not specify exactly how to classify damaged fuel. Instead the regulations license/certify packages that specify approved contents. Damaged fuel must be included in the approved contents to be a viable option. In many cases damaged SNF is encapsulated in a damaged fuel can to ensure it can confine gross fuel particles, debris, and or damaged assemblies to a known volume within a loaded cask. This damaged fuel can may then be utilized in the same way as an assembly in a storage and transportation design system. Some storage cask systems utilize top and bottom plugs to confine debris in damaged fuel. The most recent domestic documentation on damaged SNF was performed by the U.S. Energy Information Administration (EIA) and used data from U.S. reactors concluding in June 30th 2013 to produce Form GC-859, “Nuclear Fuel Data Survey”. Based on this form there were 136,821 boiling water reactor (BWR) spent nuclear fuel (SNF) assemblies and 104,647 pressurized water reactor (PWR) SNF assemblies for a total of 241,468 SNF assemblies in the U.S. Out of these 241,468 SNF assemblies, 4,521 assemblies were classified as failed. Some assemblies were also disassembled and the fuel rods or pieces of fuel rods were combined together to make a consolidated assembly. A consolidated assembly may include damaged fuel, or it could have been consolidated as part of a demonstration project. The GC-859 data includes 2,550 consolidated assemblies containing between 0 and 264 whole fuel rods. These consolidated assemblies could be placed in single assembly canisters and stored in the spent fuel pool. For dry storage and transportation, a single assembly canister is generally placed in a damaged fuel can. In addition to the consolidated assemblies, 2,391 uncanistered fuel rod pieces exist, which were removed from 494 assemblies.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Progress Towards the Reality of Low-Temperature Critical Experiments with Low-Temperature TEX

Integral benchmarks for criticality safety do not exist below room temperature. Incredible support for below room temperature benchmarks is present across the international community. Validation of low-temperature neutron cross sections is necessary to verify any operation at temperatures below room temperature which is typically observed in environments far from the equator. For example, a fissile material transportation truck may routinely observe ambient temperatures down to -40°C, which is the lower temperature bound of the normal conditions of transportation defined in the United States Title 10 Code of Federal Regulations §71.71c2. Similarly, the IAEA, which contains 176 member states, has the same requirement for subcriticality for fissile material transportation. Additionally, sub-room temperature benchmarks can validate newly produced cross sections, that include novel thermal scattering laws, from North Carolina State University. A six-case experiment designed based off the TEX-HEU baseline experiments has been proposed utilizing a chilled vacuum chamber capable of cooling the HEU stack down to -50°C. The six proposed configurations span the entire neutron fission energy range and can be tested at intermediate temperatures between room temperature and -40°C. Of the six configurations, the fastest case utilizes nearly 135 kg of HEU. Understanding the system is required prior to critical experiment operations. In order to test the functionality of the system, testing with surrogate fuel materials has been conducted. The following discusses the design of the low temperature TEX (LT-TEX) experiment as well as the progress made via surrogate testing towards a reality in which low-temperature integral benchmarks exist in the International Criticality Safety Benchmark Evaluation Project (ICSBEP).

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

PEEL-AND-STICK SENSORS POWERED BY DIRECTED RF ENERGY

PARC, a Xerox Company, is developing a low-cost system of peel-and-stick wireless sensors that will enable widespread building environment sensor deployment with the potential to deliver up to 30% energy savings. The system is embodied by a set of RF hubs that provide power to automatically located sensor nodes, and relay data wirelessly to the building management system (BMS). The sensor nodes are flexible electronic labels powered by rectified RF energy transmitted by an RF hub and can contain multiple printed and conventional sensors. The system design overcomes limitations in wireless sensors related to power delivery, lifetime, and cost by eliminating batteries and photovoltaic devices. Sensor localization is performed automatically by the inclusion of a programmable multidirectional antenna array in the RF hub. Comparison of signal strengths while the RF beam is swept allows for sensor localization, reducing installation effort and enabling automatic recommissioning of sensors that have been relocated, overcoming a significant challenge in building operations. PARC has already demonstrated wireless power and temperature data transmission up to a distance of 20m with less than one minute between measurements, using power levels well within the FCC regulation limits in the 902-928 MHz ISM band. The sensor’s RF energy harvesting antenna achieves high performance with dimensions below 5cm x 9cm.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Developmental exposure to 2,3,7,8 tetrachlorodibenzo-p-dioxin attenuates capacity of hematopoietic stem cells to undergo lymphocyte differentiation

The process of hematopoiesis, characterized by long-term self-renewal and multi-potent lineage differentiation, has been shown to be regulated in part by the ligand-activated transcription factor known as the aryl hydrocarbon receptor (AHR). 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), a ubiquitous contaminant and the most potent AHR agonist, also modulates regulation of adult hematopoietic stem and progenitor cell (HSC/HPC) homeostasis. However, the effect of developmental TCDD exposure on early life hematopoiesis has not been fully explored. Given the inhibitory effects of TCDD on hematopoiesis and lymphocyte development, we hypothesized that in utero exposure to TCDD would alter the functional capacity of fetal HSC/HPCs to complete lymphocyte differentiation. To test this hypothesis, we employed a co-culture system designed to facilitate the maturation of progenitor cells to either B or T lymphocytes. Furthermore, we utilized an innovative limiting dilution assay to precisely quantify differences in lymphocyte differentiation between HSC/HPCs obtained from fetuses of dams exposed to 3 μg/kg TCDD or control. We found that the AHR is transcribed in yolk sac hematopoietic cells and is transcriptionally active as early as gestational day (GD) 7.5. Furthermore, the number of HSC/HPCs present in the fetal liver on GD 14.5 was significantly increased in fetuses whose mothers were exposed to TCDD throughout pregnancy. Despite this increase in HSC/HPC cell number, B and T lymphocyte differentiation is decreased by approximately 2.5 fold. These findings demonstrate that inappropriate developmental AHR activation in HSC/HPCs adversely impacts lymphocyte differentiation and may have consequences for lymphocyte development in the bone marrow and thymus later in life.

60 APPLIED LIFE SCIENCES↗

Unexpected role of the IMD pathway in Drosophila gut defense against Staphylococcus aureus

Highlights: • Drosocrystallin (Dcy) is involved in resistance against oral infection with S. aureus in adult Drosophila. • The presence of S. aureus does not trigger reactive oxygen species (ROS) production in the gut. • The IMD pathway is required for the clearance of ingested S. aureus. In this study, fruit fly of the genus Drosophila is utilized as a suitable model animal to investigate the molecular mechanisms of innate immunity. To combat orally transmitted pathogenic Gram-negative bacteria, the Drosophila gut is armed with the peritrophic matrix, which is a physical barrier composed of chitin and glycoproteins: the Duox system that produces reactive oxygen species (ROS), which in turn sterilize infected microbes, and the IMD pathway that regulates the expression of antimicrobial peptides (AMPs), which in turn control ROS-resistant pathogens. However, little is known about the defense mechanisms against Gram-positive bacteria in the fly gut. Here, we show that the peritrophic matrix protects Drosophila against Gram-positive bacteria S. aureus. We also define the few roles of ROS in response to the infection and show that the IMD pathway is required for the clearance of ingested microbes, possibly independently from AMP expression. These findings provide a new aspect of the gut defense system of Drosophila, and helps to elucidate the processes of gut-microbe symbiosis and pathogenesis.

60 APPLIED LIFE SCIENCES↗

Acarbose impairs gut Bacteroides growth by targeting intracellular glucosidases

ABSTRACT Acarbose is a type 2 diabetes medicine that prevents dietary starch breakdown into glucose by inhibiting host amylase and glucosidase enzymes. Numerous gut species in theBacteroidesgenus enzymatically break down starch and change in relative abundance within the gut microbiome in acarbose-treated individuals. To mechanistically explain this observation, we used two model starch-degradingBacteroides,Bacteroides ovatus(Bo), andBacteroides thetaiotaomicron(Bt). Bt growth on starch polysaccharides is severely impaired by acarbose, whereas Bo growth is much less affected by the drug. TheBacteroidesuse a starch utilization system (Sus) to grow on starch. We hypothesized that Bo and Bt Sus enzymes are differentially inhibited by acarbose. Instead, we discovered that although acarbose primarily targets the Sus periplasmic GH97 enzymes in both organisms, the drug affects starch processing at multiple other points. Acarbose competes for transport through the TonB-dependent SusC proteins and binds to the Sus transcriptional regulators. Furthermore, Bo expresses a non-Sus GH97 (BoGH97D) when grown in starch with acarbose. The Bt homolog, BtGH97H, is not expressed in the same conditions, nor can overexpression of BoGH97D complement the Bt growth inhibition in the presence of acarbose. This work informs us about unexpected complexities of Sus function and regulation inBacteroides, including variation between related species. Furthermore, this indicates that the gut microbiome may be a source of variable response to acarbose treatment for diabetes. IMPORTANCE Acarbose is a type 2 diabetes medication that works primarily by stopping starch breakdown into glucose in the small intestine. This is accomplished by the inhibition of host enzymes, leading to better blood sugar control via reduced ability to derive glucose from dietary starches. The drug and undigested starch travel to the large intestine where acarbose interferes with the ability of some bacteria to grow on starch. However, little is known about how gut bacteria interact with acarbose, including microbes that can use starch as a carbon source. Here, we show that two gut species,Bacteroides ovatus(Bo) andBacteroides thetaiotaomicron(Bt), respond differently to acarbose: Bt growth is inhibited by acarbose, while Bo growth is less affected. We reveal a complex set of mechanisms involving differences in starch import and sensing behind the different Bo and Bt responses. This indicates the gut microbiome may be a source of variable response to acarbose treatment for diabetes via complex mechanisms in common gut microbes.

Microbiology↗

Development of Integrated Biomimetic Framework with Intelligent Monitoring, Cognition and Decision Capabilities for Control of Advanced Energy Plants

The objective of the project entitled “Development of Integrated Biomimetic Framework with Intelligent Monitoring, Cognition, and Decision Capabilities for Control of Advanced Energy Plants” is to develop algorithms and methodologies for designing a biomimetic control system for optimal control of advanced energy plants. Traditionally, control systems have been designed based on assumed a priori knowledge of the process system. A number of variables are manipulated to accomplish disturbance rejection and/or servo control performance. While it is possible to adapt the process model based on available data, the control structure and the controllers rarely change. Thus, the knowledge learnt during process operation is lost or remains underutilized. In addition, the business models of today, especially for power plants, are rapidly changing. The power plant operators are being pushed to the edge due to the penetration of renewable energy derived power to the grid, the demand for high efficiency, ever-tightening environmental regulations, and requirements for increased plant availability. Thus, power plant operations need to be agile and should adapt quickly to the dynamic requirements. A number of characteristics distinguish biological systems from the traditional process control systems. Self-organization, distributed intelligence, adaptability, intelligent monitoring, cognition, and decision capabilities are some of the powerful characteristics of the biological world that can be effectively utilized in process control. At the top, the central nervous system (CNS) integrates the information from and coordinates the activities of all parts of the bodies (for bilaterian animals). Inspired by these distinct characteristics, this project seeks to develop methodologies and algorithms to accomplish: (i) self-organization of the control structure for maximizing the plant’s operating profit by mimicking the function of the cortical areas in the human brain, (ii) distributed and adaptive controllers that mimic the rule of pursuit present in ants, (iii) intelligent monitoring of the controllers powered with cognition and decision capabilities that mimic the artificial immune systems, and (iv) seamless coordination and integration in the control system that mimics the CNS.

20 FOSSIL-FUELED POWER PLANTS↗

On the Growth and Saturation of the Gyroresonant Streaming Instabilities

The self-regulation of cosmic-ray (CR) transport in the interstellar and intracluster media has long been viewed through the lenses of linear and quasi-linear kinetic plasma physics. Such theories are believed to capture the essence of CR behavior in the presence of self-generated turbulence but cannot describe potentially critical details arising from the nonlinearities of the problem. We utilize the particle-in-cell numerical method to study the time-dependent nonlinear behavior of the gyroresonant streaming instabilities, self-consistently following the combined evolution of particle distributions and self-generated wave spectra in one-dimensional periodic simulations. We demonstrate that the early growth of instability conforms to the predictions from linear physics, but that the late-time behavior can vary depending on the properties of the initial CR distribution. We emphasize that the nonlinear stages of instability depend strongly on the initial anisotropy of CRs—highly anisotropic CR distributions do not efficiently reduce to Alfvénic drift velocities, owing to reduced production of left-handed resonant modes. We derive estimates for the wave amplitudes at saturation and the timescales for nonlinear relaxation of the CR distribution and then demonstrate the applicability of these estimates to our simulations. Bulk flows of the background plasma due to the presence of resonant waves are observed in our simulations, confirming the microphysical basis of CR-driven winds.

79 ASTRONOMY AND ASTROPHYSICS↗

Single-Phase Active Boost Rectifier with Power Factor Correction for Wireless Power Transfer Applications

Wireless Power Transfer (WPT) technology is a novel research area in the charging technology that bridges utility and the automotive industries. There are various solutions that are currently being evaluated by several research teams to find the most efficient way to manage the power flow from the grid to the vehicle energy storage system. There are different control parameters that can be utilized to compensate for the change in the impedance. To understand the power flow through the system this paper presents a novel approach to the system model and the impact of different control parameters on the load power. The implementation of an active front-end rectifier on the grid side for power factor control and voltage boost capability for load power regulation is also discussed.

PEV charging↗

Self-regulating power management for a neural network system

A neural network runs a known input data set using an error free power setting and using an error prone power setting. The differences in the outputs of the neural network using the two different power settings determine a high level error rate associated with the output of the neural network using the error prone power setting. If the high level error rate is excessive, the error prone power setting is adjusted to reduce errors by changing voltage and/or clock frequency utilized by the neural network system. If the high level error rate is within bounds, the error prone power setting can remain allowing the neural network to operate with an acceptable error tolerance and improved efficiency. The error tolerance can be specified by the neural network application.

Kegel, Andrew G.↗