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All sourcesNASA NTRSDOE OSTI
NASA NTRS2022

Nutrient Limitation Predisposes A Cultivate of Burkholderia Contaminans From the ISS Water Processor Assembly to Biofilm Formation Under Simulated Microgravity.

The International Space Station (ISS) Water Processor Assembly (WPA) experiences intermittent dormancy between water recycling events thus promoting biofilm formation within the system. In this work we aimed to gain a deeper understanding of the impact of nutrient limitation on bacterial growth and biofilm formation under microgravity in support of biofilm mitigation efforts in exploration water recovery systems. A representative species of bacteria that is commonly cultured from the ISS WPA was cultured in an WPA influent water ersatz formulation tailored for microbiology studies. Burkholderia contaminans was cultured under a simulated microgravity (SµG) treatment in a vertically rotating high-aspect rotating vessel (HARV), with a rotating control (R) in the horizontal plane at the determined optimal rpm of 15 along with a stationary (S) control. At different time points, the bacterial culture and ersatz were harvested for bacterial counts, transcriptomic and nutrient content analyses. Under the test conditions, the culture under SµG treatment consumed the essential nutrients faster than the R and S control cultures in the early stage of growth, thus approaching a nutrient limited growth condition earlier than the controls. The rapid uptake and subsequent depletion of essential nutrients was further illustrated in the transcriptomic response of the SµG culture when compared to the transcriptomic response of the R and S control conditions. The observed starvation response may serve as one element to explain a moderate enhancement of biofilm formation in the SµG treatment. One implication of this investigation is that biofilm mitigation in the ISS environment could be supported by ensuring a steady flow of water as a vehicle for essential nutrients within the WPA to avoid complete consumption which occurs in times of no flow lending to undesired biofilm formation.

Angie Diaz↗
NASA NTRSDate not supplied

Habitability Assessments And Lessons-learned From 3-day And 11-day Enriched Oxygen Hypobaric Chamber Tests At NASA Johnson Space Center

INTRODUCTION: Decompression sickness (DCS) is a risk to the health and performance of astronauts and high-altitude aircrew. Tolerance to flammability, hypoxia, prebreathe duration, and DCS risk varies across different organizations, vehicles, suits, and destinations, necessitating a variety of DCS risk mitigation approaches. Existing models of altitude DCS risk are often insufficient to enable accurate risk-informed decisions during hardware development, mission planning, and flight operations. METHODS: NASA completed outfitting of a dedicated facility at Johnson Space Center to support testing of up to eight human subjects for multiple days in hypobaric and enriched oxygen atmospheres. The primary purpose of the testing capability is validation of DCS risk mitigation protocols for Artemis missions to the Moon; however, it will also support development and validation of a generalizable altitude DCS risk estimation tool. A 3-day and an 11-day prebreathe validation test were completed in 2022, each with 8 human subjects living at 56.5 kPa (8.2 psia), 34% O2, 66% N2, with 5 simulated EVAs performed on masks at 29.6 kPa (4.3 psi), 85% O2, 15% N2. Facility and organizational lessons-learned and process improvements were recorded during and following the tests, and subjective habitability ratings were recorded daily during the 11-day test. Hypoxia and DCS-related physiological and cognitive outcome measures were recorded during both tests and are reported in companion presentations. RESULTS & DISCUSSION: All subjects completed each of the tests. Primary habitability issues related to mask discomfort during simulated EVAs and poor sleep quality due to thin mattresses. Polybenzimidazole (PBI) clothing was worn by all subjects due to the increased fire risk and may be required for Artemis missions; clothing was found to be acceptable overall with the worst ratings being due to poor fit and inelasticity. Chamber O2 and CO2 sensor inconsistency was observed that did not result in test termination but required post-test follow-up. Forward plans include additional hypobaric testing and integration of existing and future physiological outcome data into an open-source Aerospace Estimation Tool for Hypobaric Exposure Risk (AETHER). NASA is also working to make the testing capability available to commercial companies.

Andrew F J Abercromby↗
NASA NTRSDate not supplied

Early Radiometric Performance of Landsat-9 Thermal Infrared Sensor

Landsat-9, launched on September 27, 2021, carries the Thermal Infrared Sensor (TIRS). The Landsat-9 TIRS is a close copy of the Landsat-8 TIRS instrument; it is a two spectral-band, pushbroom sensor with three Sensor Chip Assemblies (SCAs) that cover the 15-degree field-of-view. The primary radiometric change between the instruments is the addition of baffling in the Landsat-9 TIRS telescope to mitigate the stray light issue that has impacted the radiometric quality of Landsat-8 TIRS. The on-orbit radiometric performance is monitored using the on-board variable temperature blackbody and views of deep space. Maneuvers to look at and around the moon have provided an assessment of the stray light. The absolute calibration is monitored by vicarious calibration techniques by teams at NASA/Jet Propulsion Lab and the Rochester Institute of Technology. Landsat-9 completed a three-month commissioning phase in January 2022 and has been operational since February 2022. The instrument has demonstrated excellent radiometric performance, as assessed from the on-orbit measurements. The TIRS instrument is radiometrically stable to 0.1% within a power cycle, and has noise levels below 0.1K. The lunar scans and the vicarious calibration data provide evidence that the stray light has been effectively mitigated.

Landsat-9↗
NASA NTRS2022

Biofilm Study Under Simulated Microgravity

The goal of this study was to understand biofilm formation under microgravity (µg), in support of biofilm mitigation efforts in exploration water recovery systems. The technical approach was to conduct a mass transfer and bacterial culture study under both simulated µg and ambient gravity. The aim was to correlate nutrient consumption to gene expression to better understand biofilm formation. A representative species of bacteria that is commonly cultured from the International Space Station (ISS) Water Processor Assembly (WPA) was cultured in a WPA influent water ersatz formulation that is tailored for microbiology studies. A mass transfer rate study was carried out using the ersatz WPA influent water by introducing a water-soluble dye to represent dissolved nutrients and nutrient particles. Imaging of dye diffusion over time allowed for the comparison of mass transport rates under a series of rotation per minute (RPM) speeds for the High Aspect Ratio Vessels (HARVs) on a Rotating Wall Vessel (RWV). This was done to determine the speed that will most accurately simulate the low convective rates experienced under actual µg conditions. Three biological replicates of the Burkholderia contaminans (B. contaminans) microbe were cultured under simulated µg with a rotating (R) control in the horizontal plane at the determined optimal RPM of 15, along with a stationary (S) reference culture. At T=0, and then at T=1,2,3 (in exponential phase) and T=4 (in S phase), the bacterial culture and ersatz were harvested for transcriptomic and nutrient content analysis, respectively. The experimental results illustrated that phosphate is a limiting nutrient in the WPA ersatz formula. Nutrient analysis illustrated that the µg treatment culture took up essential nutrients more rapidly than the R and S control cultures, yet non-essential nutrients remained higher in the µg treatment than in the controls at later timepoints. The rapid uptake and subsequent starvation of phosphate in the culture under µg conditions is further illustrated in the transcriptomic response when compared to that of the R control condition. The subsequent starvation response may serve as one element to explain a moderate enhancement of biofilm formation in the µg treatment. One implication of this work is that biofilm mitigation in the ISS environment could be supported by ensuring a steady flow of water as a vehicle for phosphate within the WPA to avoid complete phosphate consumption, which occurs in times of no flow and leads to undesired biofilm formation.

Aubrie O’Rourke↗
NASA NTRSDate not supplied

CDC Bioreactor Configuration Method for Volume Level Control with Controlled Inlet and Outlet Flow

Environmental Control Life Support Systems and other microbiological biofilm studies often utilize small scale bioreactors. Among these options are the popular BioSurface CDC bioreactors, currently being utilized by Marshall Space Flight Center groups researching the impact of biofilms on life support systems. After a recent experimental regime, it was determined additional equipment could be added to augment the capabilities of the bioreactors. Previous research configurations such as ASTM E3161 – 18 relied on an outlet stream gravity draining from a side drain port located above sampling coupons. This limited applications to experimental conditions with a controlled inlet and uncontrolled outlet flow. With the introduction of a small single pole single throw (SPST) reed float switch, a peristaltic pump connected to the outlet drain and a chassis controller, the bioreactor is able to maintain a set level. The modification allows additional variables to be tested, including highly adjustable fill and flush cycles, bioreactor volume, draining and filling control. Once the configuration modifications were implemented through the installation of the new equipment, data was collected to ensure the stability of the level measurements. As the level control switch is a float switch, consideration was taken into account for effects of internal stirring speed, along with effects of inlet and outlet flow rate. Data presented in this study will illustrate the stability and effectiveness of the configuration changes in equipment made to the bioreactor. These configuration changes are proving an effective control method to conduct biofilm mitigation techniques for ECLSS hardware research. The controlled level capabilities allow for a constant drip feed flow rate into the bioreactors, a key aspect of the biofilm mitigation testing.

CDC Bioreactor↗
NASA NTRSDate not supplied

Mapping Forest Carbon Stocks to Understand Carbon Implications of Treatment on Wildfire for the Calwood Fire, Boulder County Colorado

Recent, record-breaking wildfire activity in the western U.S. illustrates the need for fire mitigation, such as forest fuels reduction treatments. Forests serve as crucial carbon sinks that combat the increasing effects of climate change, but fuels reduction treatments may remove carbon from forested systems. As a result, forest managers need to find a balance between fire mitigation and carbon preservation. This project partnered with Boulder County Parks and Open Space (BCPOS) and the University of Colorado, Denver to investigate the 2020 Cal-Wood fire in Boulder County, Colorado. Using remote sensing data from Landsat 8 Operational Land Imager, Shuttle Radar Topography Mission, Sentinel-2 MultiSpectral Imagery, and LiDAR, we mapped post-fire forest carbon pools and compared these values with values derived from field measurements. The analysis suggested that fuels reduction treatments did not reduce carbon loss in the presence of wildfire enough to clearly distinguish post-fire carbon between treated and untreated areas. However, the final carbon maps provide BCPOS and researchers with an opportunity to explore carbon estimation models based on remote sensing data as well as a framework to evaluate fuels reduction treatment effectiveness and impact on forest carbon stocks for future wildfire events.

Sarah Hettema↗
NASA NTRSDate not supplied

Assessing Environmental and Socioeconomic Factors of Urban Flood Vulnerability in Kansas City, Kansas

Pluvial flooding, over-saturated ground, and drainage systems disproportionately impact historically marginalized urban neighborhoods during extreme rainfall events. These communities are impacted by physical and socioeconomic factors that make them vulnerable to flooding events, such as high concentrations of impervious landcover, high precipitation rates, and a combined sewer system framework. Despite known vulnerability to environmental hazards, understanding potential pluvial street-level flooding events are largely unknown. Using the open-source National Capital Project’s Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) Urban Flood Risk Mitigation model, NASA DEVELOP examined neighborhood scale runoff retention and potential economic damages for risk mapping throughout Kansas City, Kansas. The generated outputs aid in identifying vulnerable neighborhoods susceptible to flooding and in need of future intervention. Previous studies have applied this model framework to understand urban flood vulnerability regarding ecosystem services, urban planning, and flood mitigation strategies. We utilized the InVEST outputs to develop indices pertaining to environmental justice factors of race, socioeconomic status, social vulnerability, and health. The findings indicate that historically redlined neighborhoods in Kansas City, Kansas face disproportional impacts from flood events and are subject to greater environmental stressors. This research provides an approach to utilizing an open-source flood vulnerability model to empower neighborhood-scale environmental justice analysis, enhancing the local communities' understanding of present-day impacts of historical environmental injustices.

Hadwynne Gross↗
NASA NTRS2023

Concept of Operations for an In-time Aviation Safety Management System (IASMS) for Upper E Airspace

The National Airspace System undergoes continuous change including in the Upper Class E airspace involving increasingly complex operations and a widening diversity of vehicles. To secure a safe future system, the National Academies recommended an In-time Aviation Safety Management System (IASMS) that is extensible to Upper E. Current Air Traffic Management is not cost-effective to scale for future Upper E operations and diversity of vehicles so the Federal Aviation Administration developed an Upper E Traffic Management ConOps to safely integrate the diverse operations and vehicles having different performance characteristics and flight missions without disrupting current operations including space launch and reentry, suborbital flights, supersonic and hypersonic flights, slow moving or stationary unmanned balloons, and long endurance fixed wing vehicles that are slow, stationary, or high speed. IASMS integrates state-of-the-art predictive modeling with reactive and proactive analytics to detect hazards and mitigate risk precursors for Upper E operators. IASMS identifies emergent safety risks exposed by transformation of the NAS with new and increasingly complex operations. Safety intelligence will also expand the data available and offer insight to new approaches for implementing safety improvements to mitigate risk with more seamless “in-time” integration across the policy, risk management, safety assurance, and promotion pillars of SMS.

K Ellis↗
NASA NTRSDate not supplied

Operational Techniques in Microgravity for Cryogenic Fluid Management

Management of cryogens in microgravity is a critical challenge for exploration missions to the Moon and Mars. Operational strategies to aid in cryogenic fluid management (CFM) are proposed and demonstrated using computational fluid dynamics simulations. Application of axial settling thrust is an essential – but potentially expensive – tool for CFM. Demonstrations show that its utilization and timing are important during certain unsettling events, such as engine cutoff. At other times, substantial savings can be realized by reducing the average acceleration (through pulsing thrusters, for example). If the metric for reaching a settled condition is a particular number of slosh periods, then fewer settling resources are needed with a lower acceleration. Alternatively, if a target wave amplitude is the settling objective, then a lower average acceleration will also allow for a higher allowable amplitude for the same heat transfer. Savings can also be obtained by rolling the vehicle, which can mitigate lateral slosh. Advantages and disadvantages of anti-slosh baffles are also discussed along with practical examples of their impact on CFM and ullage collapse. Ullage collapse examples are presented along with recommendations for slewing a vehicle with angular and translational acceleration to mitigate mixing between liquid and ullage.

Cryogenic Fluid Management↗
NASA NTRSDate not supplied

Consequences of Asteroid Characterization on the State of Knowledge about Inferred Physical Properties and Impact Risk

Physical characteristics of Near-Earth Objects (NEOs) are essential inputs to planetary defense assessments. The size, density, and strength of an NEO are critical inputs to modeling behavior during atmospheric entry as well as assessing the risk of impact. Similarly, knowledge of the physical characteristics of an object are necessary to evaluate the probable result of a mitigation mission. Usually, these attributes cannot be directly measured, but increasingly sophisticated methods have been developed to infer physical properties from related measurements of asteroids, meteors, and/or meteorites. Fortuitously, some of these measurements have been obtained for enough NEOs to elucidate the distribution of values across the sampled population. However, the situation becomes more challenging when considering a specific asteroid, since it is unlikely that all the relevant measurements have been made for any given object. We have developed a Bayesian network that can combine available information about a particular NEO with knowledge of the larger population to infer probabilistic values and uncertainties for physical characteristics of interest. Distributions of asteroid population albedos, taxonomic classes, and macroporosities, along with meteorite density distributions and associations between taxonomic classes and meteorite classes, provide the default distributions for the network’s parameter nodes. The inference network links parameters for each virtual asteroid either deterministically or probabilistically as appropriate, and eliminates any unphysical combinations of parameters. Within the context of planetary defense, our Bayesian network can be used to constrain the ranges of likely impactor properties, which can subsequently reduce the uncertainty in modelling of atmospheric entry, mitigation efficacy, and impact risk assessment. When additional measurements become available for a specific object, the network incorporates those measurements to generate virtual asteroids with property distributions that are consistent with the measurements. We will use the 2023 PDC scenario to demonstrate how the inference network can be combined with plausible characterization measurements to refine the state of knowledge about likely combinations of physical parameters and the resulting impact risk.

risk assessment↗
NASA NTRSDate not supplied

Temporal Changes in Astronauts’ Muscle and Cardiorespiratory Physiology Before, During, and After Spaceflight

Background: NASA’s planned space exploration missions will require astronauts to safely perform extravehicular activity (EVA) and to safely egress vehicles in a variety of landing scenarios. Prolonged exposure to spaceflight can diminish tolerance for physical activity, decrease cardiovascular and sensorimotor function, cause loss of bone mineral density, as well as reduced muscle mass and strength. Although exercise can mitigate these spaceflight-induced physiological decrements, little is known regarding the time-course of changes in muscle and aerobic performance during spaceflight. Furthermore, these exercise countermeasures are not fully protective. For example, maximal aerobic capacity (VO2pk), lower body muscle cross-sectional area, and strength decrease by about 10% to 15% after short- (≈14 days) and long-duration (≈6 months) missions on the International Space Station (ISS). Future space missions longer in duration and further from Earth will employ exploration vehicles that will have exercise hardware with less robust and more constrained exercise capabilities than of those available on the ISS. Thus, countermeasures will need to be optimized to protect crew health and performance on exploration-class missions lasting up to 3 years. This requires a more detailed understanding of the dynamic effects of spaceflight on human health and performance, the ability of exercise to protect against this deconditioning, and the interaction of exercise with interrelated factors like nutrition, sleep, and environmental conditions. Methods: We will use standardized research and medical testing protocols previously validated in 1g and microgravity to quantify the time course and the inter-individual variability of changes in physical performance, including cardiorespiratory fitness and muscle strength and endurance, before, during, and after spaceflight missions lasting 2 months, 6 months, and 1 year. Additionally, we will use an extrapolation model to predict changes associated with multi-year exploration missions. Additionally, we will monitor in-flight exercise, nutrition, and sleep using in-flight assessment tools. Significance: Our testing protocols will provide valuable information for determining time course of change and the interindividual variability of spaceflight-induced deconditioning of aerobic capacity and muscle strength and endurance over the course of spaceflight missions up to and beyond 1 year. This information will be vital to assess whether humans can be physically ready for deep space exploration, such as on a mission to Mars, using current technology, or if additional mitigation strategies are necessary.

N Strock↗
NASA NTRSDate not supplied

Recent Improvements and Verification of A Full Body Model in Opensim

BACKGROUND: The dynamic feasibility [1,2] criterion, that the subject’s Center of Pressure (COP) be located within the Base of Support (BOS) which outlines the feet, has aided in assessing the stability of human motion recorded on earth while performing the recorded tasks in lunar gravity or as countermeasures exercises on a vibration isolation and stabilization system in microgravity. The convex hull of the BOS on the platform under the subject’s feet was estimated using virtual markers on the feet of the scaled subject model. The COP was calculated using the ground reaction forces and moments determined from motion capture data with biomechanical modeling tools [3]. Occasionally, large-amplitude oscillatory spikes or “artifacts” were observed in the subject’s linear and angular momentum derivatives, affecting some COP data derived from motion capture. The purpose of this investigation was to assess and improve the accuracy of model scaling and BOS estimation as well as to determine the efficacy of model adjustments in mitigating artifacts influencing motion capture-derived ground reaction force and COP results. METHODS: To aid evaluation of proposed process and model updates, motion capture data were collected for two subjects during unit test and range of motion trials, lunar tasks, and countermeasures exercise motions. Markers were added to the full body Plug-in Gait marker set [4] during data collection. New markers were placed on the front, back, sides, and top of the head to improve scaling using distances between marker pairs. Medial elbow markers were added to stabilize the upper arm during OpenSim Inverse Kinematics (IK) [5]. Finally, markers were added on the outer edge of the heels and on the outside edges of the first and last toes on each foot. These additional foot markers were made available to test new automated foot scaling techniques and to calculate the error between the subject’s estimated and recorded BOS. The modified unscaled OpenSim Full Body Rajagopal Model [6,7] was adjusted using some previously investigated techniques [8] to mitigate rapid shifts in joint angles occurring during IK, as these were found to cause the spike artifacts observed in subsequent stages of analysis. Since OpenSim models use Euler angles and rotation sequences, the arm axes of rotation were adjusted, and the pelvis order of rotation was changed to minimize the likelihood of encountering “gimbal lock” during common human motion. The model clavicle, arm, elbow, wrist, pelvis, and ankle angle limits were adjusted to better accommodate the full human range of motion seen in exercise and lunar data. The shoulder joint center was calculated using a “pivoting” algorithm [9], and both shoulder joint center and upper arm markers were included during IK to provide additional shoulder stability on a case-by-case basis. The quality of IK results was assessed by three criteria: minimizing error between recorded motion capture markers and model markers, checking for reasonable rates of change in joint angles between fames (i.e., no IK artifacts), and ensuring the absence of spikes in the inertial forces and angular momentum derivatives calculated using a custom OpenSim plugin [10]. RESULTS: The additional markers placed on the subject during data collection allowed the head and feet to be scaled more accurately using distances between new marker pairs. Scaling with BOS markers placed on the subject and removing the limit on subtalar angle resulted in more accurate BOS determination. Unrealistically large changes in joint angles between frames could be reduced by including clavicle, sternum, and medial elbow markers during IK. In cases with large arm ranges of motion, results could be further improved by running IK using medial elbow and virtual shoulder joint center markers. Model adjustments significantly improved the IK results affecting COP calculation and increased the accuracy of BOS estimation.

C A Bell↗
NASA NTRS2023

Climate and Human Impacts on Hydrological Processes and Flood Risk in Southern Louisiana

Satellite observations of coastal Louisiana indicate an overall land loss over recent decades, which could be attributed to climate and human-induced factors, including sea level rise (SLR). Climate induced hydrological change (CHC) has impacted the way flood control structures are used, altering the spatiotemporal water distribution. Based on “what-if” scenarios, we determine relative impacts of SLR and CHC on increased flood risk over southern Louisiana and examine the role of water management, via flood control structures, in mitigating flood risk over the region. Our findings show that CHC has increased flood risk over the past 28 years. The number of affected people increases as extreme hydrological events become more exceptional. Water management reduces flood risk to urban areas and croplands, especially during exceptional hydrological events. For example, currently (i.e., 2016-2020 period), CHC-induced flooding puts an additional 73km2 of cropland under flood risk at least half of the time (median flood event) and 65km2 once a year (annual flood event), when compared to a past period (1993-1997). A ten- to twenty-fold increase relative to SLR-induced flooding. CHC also increases population vulnerability in southern Louisiana to flooding; additional 9900 residents currently live under flood risk at least half of the time, and that number increases to 27,400 for annual flood events. Residents vulnerable to SLR induced flooding is lower (6000 and 3300 residents, respectively). Conclusions are that CHC is a major factor that should be accounted for flood resilience and that water management interventions can mitigate risks to human life and activities.

Augusto Getirana↗
NASA NTRSDate not supplied

PLANETARY DEFENSE: FINDINGS AND RECOMMENDATIONS FROM THE NATIONAL ACADEMIES PLANETARY SCIENCE AND ASTROBIOLOGY DECADAL SURVEY 2023 - 2032

The National Academies of Sciences, Engineering, and Medicine was chartered by the NASA Science Mission Directorate (SMD), with support from the National Science Foundation, to conduct a new decadal survey to develop a comprehensive science and mission strategy for planetary science (1). The survey was tasked to determine the current state of knowledge and to identify the most important scientific questions to be addressed in astrobiology and planetary science during the upcoming decade of 2023 - 2032. For the first time, the decadal survey also included and addressed the topic of planetary defense, now recognized as a key element of NASA’s planetary science activities under the direction of NASA’s SMD Planetary Defense Coordination Office. The topic of planetary defense was included in the decadal survey as a dedicated chapter, divided into five sections based on the framework from the U.S. National NEO Preparedness Strategy and Action Plan (2). These sections discussed: NEO Detection, Tracking, and Characterization NEO Modelling, Prediction, and Integration NEO Deflection and Disruption Missions International Cooperation on NEO Preparation NEO Impact Emergency Procedures and Action Protocols Content of these sections included identifying the activities, ground- and space-based assets, facilities, resources, technologies, and missions that should be considered to enable NEO preparedness over the next decade. Supporting inputs to this chapter involved contributions from submitted white papers authored by many members of the NEO science and planetary defense communities. In addition, a planetary defense rapid mission architecture study commissioned by the decadal survey small bodies panel and led by the Jet Propulsion Laboratory examined several different types of in-situ characterization and mitigation demonstration missions. All of this input contributed to the formulation of 42 findings and 11 recommendations that suggest a path forward for increasing our understanding of the NEO population and their associated physical characteristics, modelling the impact probabilities and hazard potential of these objects, and implementing plans to develop and test NEO impact mitigation capabilities. Including planetary defense in the decadal survey has helped sharpen the focus on this international issue and provided key inputs for stakeholders and decision makers to consider when developing future planetary defense-related activities. Such information is vital for developing a long-term integrated planetary defense capability able to protect humanity in the decades to come.

NEO Detection and Characterization↗
NASA NTRSDate not supplied

CDC Bioreactor Configuration Method for Volume Level Control with Controlled Inlet and Outlet Flow

Environmental Control Life Support Systems and other microbiological biofilm studies often utilize small scale bioreactors. Among these options are the popular BioSurface CDC bioreactors, currently being utilized by Marshall Space Flight Center groups researching the impact of biofilms on life support systems. After a recent experimental regime, it was determined additional equipment could be added to augment the capabilities of the bioreactors. Previous research configurations such as ASTM E3161-21 relied on an outlet stream gravity draining from a side drain port located above sampling coupons. This limited applications to experimental conditions with a controlled inlet and uncontrolled outlet flow. With the introduction of a small single pole single throw (SPST) reed float switch and a peristaltic pump connected to the outlet drain and a chassis controller, the bioreactor is able to maintain a set level. The modification allows additional variables to be tested, including highly adjustable fill and flush cycles, bioreactor volume, draining and filling control. Once the configuration modifications were implemented through the installation of the new equipment, data was collected to ensure the stability of the level measurements. As the level control switch is a float switch, consideration was taken into account for effects of internal stirring speed, along with effects of inlet and outlet flow rate. Data presented in this study will illustrate the stability and effectiveness of the configuration changes in equipment made to the bioreactor. These configuration changes have demonstrated control method to conduct biofilm mitigation techniques for ECLSS hardware research. The controlled level capabilities allow for a constant drip feed flow rate into the bioreactors, a key aspect of the biofilm mitigation testing.

CDC Bioreactor↗
NASA NTRS2023

Molecular Identification of Microbial Contaminants

Microorganisms can have significant impacts on the success of NASA’s missions, including the integrity of materials, the reliability of scientific results, and maintenance of crew health. Robust cleaning and sterilization protocols are currently in place in NASA facilities, but agency experts agree that microbial contamination is unavoidable and its impact on NASA’s missions and science must be minimized. Therefore, it is critical to understand: 1) what specific microorganisms are present, 2) how they may impact scientific objectives, and 3) how to select appropriate mitigation strategies. The Marshall Space Flight Center (MSFC) Planetary Protection (PP) microbiology lab historically relied solely upon enumeration of culturable microbial contamination associated with spacecraft materials or cleanrooms. However, this process is time consuming, many microbes cannot be cultured, and very few can be identified with any fidelity using NASA standard microbiological methods. The work described in this white paper includes the establishment of molecular identification capabilities at MSFC, including DNA isolation, amplification, purification, and Sanger sequencing. This capability will not only improve planetary protection efforts at MSFC (i.e. by identifying contaminating microorganisms in cleanrooms or on spacecraft) but also offers a service center-wide for the identification of contaminants that arise in other projects, processing locations, or during set up and roll out of spacecraft. This work also lays the foundation for higher throughput efforts to identify large populations of microbes across the lifetime of a project and serves as the starting point for future work into whole genome sequencing, non-culture based methods, or additional characterization studies. Ultimately, accurate identification informs appropriate mitigation strategies, increasing the chances of success for NASA’s missions and objectives.

C. D. Cassilly↗
NASA NTRS2023

Optimizing Surfactant Templating of Yttria-Stabilized Zirconia Aerogels for High-Temperature Applications: Effect of Anionic and Nonionic Surfactant

Aerogels, mesoporous materials with extreme material properties including high specific surface area, low density, and low thermal conductivity, can be useful for applications including catalysis, sorption media, and drug delivery. The low thermal conductivity and high surface area of aerogels makes them advantageous as thermal management systems, specifically for aerospace or aeronautics applications. However, the pore collapse and subsequent surface area decrease of aerogels following exposure to high temperatures must be mitigated to allow aerogels to be effectively used in thermal management applications. This work investigates the anionic surfactant sodium dodecyl sulfate (SDS) and the nonionic surfactant Pluronic® P-123 as surfactant templates in yttria-stabilized zirconia (YSZ) aerogels. By utilizing surfactant templates, known to influence pore structure, it is anticipated that sintering and densification of the aerogels following high-temperature exposure will be mitigated. It was determined that the addition of SDS and P-123 increased the surface area and pore volume of as-dried aerogels and suppressed crystallite growth at high temperatures. However, the impact of surfactant templates on the surface area and pore volume of heat-treated aerogels was negligible, potentially due to low concentrations of surfactant and the removal of the surfactant following high-temperature exposure.

Yttria-stabilized zirconia aerogels↗
NASA NTRSDate not supplied

EMU CO2 Washout Comparative Assessments for the HAB/HAP-E in Support of EVA 80

After water was reported in the Extravehicular Mobility Unit (EMU) helmet during ISS US EVA 80, mitigation strategies were created to attempt to arrest the motion of any droplets that enter the helmet for future Extravehicular Activities (EVAs). This included adding absorbent materials into the interior of the helmet. But before a mitigation strategy can be implemented, it must first be proven to be safe. Towards this aim, a computational fluid dynamics (CFD) model was developed to assess the effect that the absorbent material has on the concentration of carbon dioxide in the EMU helmet. Within a small, closed volume such as a helmet, some amount of the carbon dioxide produced by the suit-wearer will be re-inhaled before being cleared from the oral-nasal region. The ability of the suit to remove carbon dioxide is referred to as CO2 washout. Pathological levels of inhaled CO2 (hypercapnia) are associated with dizziness, fatigue, and headaches. The CFD model was built in ANSYS Fluent and adapted to assess CO2 washout in a variety of scenarios grouped into three categories: ventilation cases, varying metabolic rates, and varying sorbent material configurations. The presence of the sorbent material did not prove detrimental to CO2 washout in the helmet.

Moses Navarro↗