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At least 1,477 records · Page 82

New Horizons for a Practical and Performance-Optimized Solar System Internet

The High-rate Delay Tolerant Networking (HDTN) project at the NASA Glenn Research Center (GRC) is developing a performance-optimized Delay Tolerant Networking (DTN) implementation using the Bundle Protocol (BP) both for infusion in modern, distributed spacecraft systems and for foundational network research purposes. While one purpose of networking is to enable a returns-to-scale with communicating assets, it is recognized that neither the protocol nor its implementations may impose a bottleneck on data delivery if expected to be implemented. With this in mind, this paper explores the current status of the HDTN software, including its capabilities and also various performance metrics at the bundle layer, the convergence layers (e.g. performance-optimized Licklider Transmission Protocol, or LTP), and bundle storage and retrieval. This includes goals of software implementations that support multi-gigabit per second communications regardless of payload size. A discussion on the current software architecture and internal scheduling and routing is included. Interoperability with such extant DTN implementations as Interplanetary Overlay Network (ION) and DTN Marshall Enterprise Implementation (DTNME) are included. The research goals of the project are also listed, including Software-Defined Networking (SDN) and the theory of DTNs. A table-based filtration system for the Bundle Protocol (BP), called BP Filter, is also explored: this addition enables table-based policies within the context of a DTN for management purposes. Infusion begins with systems testing in the Johnson Space Center (JSC) Software Development and Integration Laboratory (SDIL), which supports International Space Station (ISS) Flight Software development, integration, and verification. Observations made from the results of testing in the SDIL and in flight testing are also discussed, pointing to a path for infusion into high data return low-earth orbit (LEO) missions. The paper concludes with areas for future work.

Delay Tolerant Networking↗

Proposed Protocols for Defining Requirements and Sizing of Media-Based Filters for Spacecraft and Planetary Lander Applications

The air quality control equipment aboard future deep space exploration vehicles provide the vital function of maintaining a clean cabin environment for the crew and the hardware. This becomes a serious challenge in pressurized space compartments since no outside air ventilation is possible, and a larger particulate load is imposed on the filtration system due to lack of sedimentation in low gravity, and can experience short duration peak loading of dust from planetary surfaces for Lunar or Mars landers. The filter industry has established methods to properly size filters for a given particulate load, but requirements for the space or planetary application introduce additional consideration. In this work, a methodology for properly evaluating and sizing particulate filters for a Lunar surface pressurized environment will be presented, including estimating the loading and particle size distributions of the loading based on mission requirements. In addition, a scaling analysis from single filter media sheet to full-scale filters for this application, based on recent testing, will also be presented. These results of this study may provide meaningful guidance for properly designing media-based particulate filters for the air revitalization challenges in future deep space exploration missions.

media-based filters↗

Water Recovery from Bioreactor Mixed Liquors Using Forward Osmosis with Polyelectrolyte Draw Solutions

This paper reports on the use of forward osmosis (FO) with polyelectrolyte draw solutions to recover water from bioreactor mixed liquors. The work was motivated by the need for new regenerative water purification technologies to enable long-duration space missions. Osmotic membrane bioreactors may be an option for water and nutrient recovery in space if they can attain high water flux and reverse solute flux selectivity (RSFS), which quantifies the mass of permeated water per mass of draw solute that has diffused from the draw solution into a bioreactor. Water flux was measured in a direct flow system using wastewater from a municipal wastewater treatment plant and draw solutions prepared with two polyelectrolytes at different concentrations. The direct flow tests displayed a high initial flux (>10 L/m2/h) that decreased rapidly as solids accumulated on the feed side of the membrane. A test with deionized water as the feed revealed a small mass of polyelectrolyte crossover from the draw solution to the feed, yielding an RSFS of 80. Crossflow filtration experiments demonstrated that steady state flux above 2 L/m2·h could be maintained for 70 h following an initial flux decline due to the formation of a foulant cake layer. This study established that FO could be feasible for regenerative water purification from bioreactors. By utilizing a polyelectrolyte draw solute with high RSFS, we expect to overcome the need for draw solute replenishment. This would be a major step towards sustainable operation in long-duration space missions.

Calen R Raulerson↗

Overview of NASA's RESOURCE (Resource Exploration and Science of OUR Cosmic Environment) Project

The RESOURCE(Resource Exploration and Science of OUR Cosmic Environment) project is supported by NASA’s SSERVI (Solar System Exploration Research Virtual Institute) and is led by Principal Investigator (PI) Dr. Jennifer L. Heldmann and Deputy PIs Dr. Matthew Deans and Dr. Alexander Sehlke at NASA Ames Research Center. RESOURCE is focused on enabling In Situ Resource Utilization (ISRU) near the sites of robotic and/or human missions to enable sustainable and affordable exploration of the Moon and near-Earth objects (NEOs). This year RESOURCE has supported the development of a summary of the current state of knowledge regarding lunar polar volatiles as well as a comprehensive catalog and analysis of NEOs. RESOURCE is developing advanced mission capabilities to enable rapid, collaborative operations for lunar resource exploration missions. Hardware testing has been conducted to evaluate potential contaminants released during lunar polar regolith heating. Volatiles and particulates are the two sources of contamination that will affect the requirements of a water cleanup system, and RESOURCE research has identified the requirement for filtration prior to electrolysis for ISRU. RESOURCE also supports development of next-generation planetary drilling systems with integrated instrumentation within the drill. RESOURCE is also deeply committed to sustained efforts to engage educators, students, and broadening participation among under represented groups, and has partnered with Howard University in Washington, DC to foster minoritized students’ interest in STEAMD (science, technology, engineering, arts, math, design) careers through direct and virtual experiences with NASA Subject Matter Experts.

Jennifer L Heldmann↗

Air Quality in the International Space Station

The International Space Station (ISS) gives a 7-member astronaut crew the ability to live and work in low Earth orbit. It is a unique indoor environment, which has served as both home and workplace to over 250 people since the year 2000. In this low gravity environment, smoke does not rise and cookie crumbs do not settle the way they do on Earth, causing aerosols to behave differently and pose unique hazards for crew members. In its existence, virtually the same volume of ISS air has been continuously conditioned and ‘revitalized,’ including the removal of particles by filtration. Particles have been sampled and measured in real-time through ISS technology demonstration payloads, and the results differ vastly from typical terrestrial indoor spaces. This presentation will cover particle types, quantities and sources in the unique ISS environment.

Marit E. Meyer↗

Environmental Testing of Contamination Sensitive Instruments and Optics in Non-Cleanliness Controlled Facilities

The James Webb Space Telescope (JWST) Optical Telescope Element + Integrated Science Instrument Module (OTIS) went through environmental testing at the Goddard Space Flight Center. The vibration and acoustic tests occurred outside of cleanrooms. These areas are typically around ISO Class 8, far out of specifications for JWST. Through collaboration between the OTIS Mechanical and Contamination Engineering teams, a portable clean enclosure was designed, built, and verified to prevent contamination of the sensitive OTIS assembly. This manuscript describes the process of designing and building this enclosure, including materials selection and High Efficiency Particle Air (HEPA) filtration. This enclosure also provided environmental control with a portable air conditioning unit to control the relative humidity level enabling personnel to work within it. The manuscript emphasizes working in a systematic order to maintain cleanliness and integrity. “Clean as you go” during construction was a top priority to ensure that this critical Ground Support Equipment (GSE) was acceptable. The process of installing the walls and then sealing their internal and external surfaces with approved films was critical to maintaining OTIS integrity. This proved important because OTIS was in the enclosure for months longer than originally planned. This manuscript concludes by reviewing the process used to verify that the OTIS clean enclosure could achieve acceptable contamination level fallout accumulation for use during the vibration and acoustic testing and then verifying that the predicted performance was achieved after testing was complete.

Contamination Control, James Webb Space Telescope,↗

The membrane bioreactor (MBR): A hybrid technology for bioregenerative wastewater treatment and resource recovery in space

Extraplanetary surface habitat life support systems (LSS) on the Moon and Mars, as well as long-duration space travel, will require novel capabilities to withstand anticipated unique, harsh conditions. In order to provide safe, habitable environments for the crew, water purification and waste processing systems will be required to treat all sources of water (condensate, Sabatier, urine, hygiene, fecal, food waste) in order to achieve the necessary levels of recovery needed to sustain life over the long-duration missions. The ability to recycle organic wastes creates an opportunity to recover critical elements (e.g., C, H, O, N, P) for subsequent food production, water purification, and atmospheric regeneration. Bioregenerative systems mimic functions of nature in engineered systems, or bioreactors, utilizing combination of prokaryotes, eukaryotes and archaea. While these systems are commonly used on Earth for wastewater treatment, bioreactors for space travel face additional challenges. Terrestrial bioreactors often rely on gravitational settling of dense flocs and granules for cell retention. For micro- or partial-gravity environments, density differential alone will not be adequate for cell retention; a gravity-independent means for cell retention is crucial. The membrane bioreactor represents the state of the art in wastewater treatment. This hybrid system combines biological processes with membrane filtration to achieve performance beyond what each can accomplish individually. The complete cell retention in an MBR allows for the decoupling of hydraulic retention time (HRT) and solids retention time (SRT), which result in a high-thruput, compact, treatment system. The Bioregenerative Water Technology Team at NASA Kennedy Space Center and the University of South Florida has developed a bioregenerative platform based on the hybrid MBR technology. The overall architecture is compact, modular, flexible, and adaptable to mission evolutions. The main subsystems of the bioregenerative architecture include: 1) Anaerobic membrane bioreactor (AnMBR): Also termed the Organic processor assembly (OPA), the function of the AnMBR is to treat organic wastes such as fecal and food wastes. These wastes are characterized by a concentration of suspended solids comprised of carbohydrates, proteins and lipids. The assigned function of the AnMBR is to break down and covert suspended solids to biogas (methane, hydrogen and carbon dioxide), reduce effluent chemical oxygen demand (COD), liberate organically-bound nutrients, and remove pathogenic organisms. 2) Phototrophic membrane bioreactor (PMBR): The PMBR is comprised of a co-culture of microalgae and bacteria. The assigned function of the PMBR is to polish the permeate of the AnMBR to further remove dissolved organic carbon, manage nutrients (nitrogen transformation, load dampening), and perform air revitalization. 3) Food processor assembly (FPA): The FPA is a food production platform (prokaryotic or eukaryotic), fueled by outputs from the AnMBR, or PMBR. For the presentation, we will describe each step of the bioregenerative architecture, and present performance data from extended trials treating analog and real metabolic wastes.

Bioreactor↗

Method Development for In Situ Microbiome Profiling of the Water Recovery System’s Wastewater Tank Onboard the International Space Station

A distinctive microbial community has inhabited the wastewater tank within the International Space Station Water Recovery System (WRS) for over 14 years and experienced the stressors associated with the microgravity environment. The WRS generates potable water for the crew from urine distillate, humidity condensate, Sabatier product water, and the occasional off-loading of ground-supplied water. The reservoir for these products, the wastewater tank, does not have a means of microbial control. While samples are occasionally collected for analysis, the time between sample collection and the return to Earth, as well as the lack of preservation, results in a skewed depiction of the microbiome. Routinely observed from these returned samples are high counts (105 – 106 colony forming units per mL) and two prevailing genera, Burkholderia and Ralstonia. The wastewater tank likely contains a more diverse microbiome, as a higher diversity of bacteria and fungus has been noted upstream and downstream of the tank. To characterize the microbial profile of the tank, analysis needs to occur at the time of sample collection. Toward this goal, a method for in situ analysis based on nanopore sequencing was developed. The filter-to-sequencer method evolved from previous work that has been validated onboard the ISS (BEST payload and the BioMole Crew Health Care Systems Facility). The method, including filtration, DNA extraction, purification, amplification, library preparation, and nanopore sequencing will be described. Additionally, data collected with this method from both ISS and terrestrial samples will be detailed. The consumables needed to support in situ analysis of the tank are set to the launch to the ISS in the spring of 2023. This investigation will allow for the first accurate characterization of the microbiome of the tank providing insight for crew health, planetary protection, and has the potential to enable engineering controls for future space station water systems.

Sarah Stahl-Rommel↗

Polymer Aerogels for Lunar Survivability (PALS)

As the Space Community endeavors to reach new heights of exploration, materials for extreme environment are on the forefront of research. One such material would include polymer aerogels; lightweight solids with nano-scale pore size (10-20 nm), high internal surface area (500-850 m2/g), low thermal conductivity (~20 mW/K), low density ( 0.15-0.05 g/cm3) and extremely high porosities (>95%). Due to these interesting properties, aerogels are ideal as thermal insulators and are by no means limited to this application. The high porosity combined with high surface area, make aerogels an ideal host system for particle incorporation for applications such as IR filtration, sensor platforms, as well as vibro-acoustic, dust, and radiation mitigation materials.

Stephanie Vivod↗

WET Water Resources: A Google Earth Engine Python API Tool to Automate Wetland Extent Mapping Using Radar Satellite Sensors for Wetland Management and Monitoring

Wetland ecosystems are annually or seasonally wet transition zones between land and water. They provide a range of ecosystem services such as water filtration, flood mitigation, and carbon sequestration, as well as hosting biodiversity hotspots. Although they fulfill fundamental physical and natural processes, wetland extent and health are threatened by anthropogenic influences related to urbanization, population increase, pollution, and climate change. Recognizing the need to quantitatively monitor changes in these recently threatened ecosystems in a timely and cost-effective way, we developed a Google Earth Engine (GEE) Python API tool for automated wetland extent mapping using optical and radar satellite sensors that can be applied globally. The tool will significantly improve wetland change analysis and monitoring as the optical and SAR data proves high resolution (5-10 m) imagery, and SAR data is unaffected by cloud cover and light availability (day vs. night), which are common limitations for other remotely sensed sensors. The tool utilizes Copernicus Sentinel-1 C-band and NISAR L-band synthetic aperture radar (SAR) imagery. During image preprocessing, we applied a MODIS snow mask product to mask global snow coverage, which would affect land classification sensitivity. Calibration and validation were conducted through a historical change and sensitivity analysis of the Sudd watershed located in central Sudan. The tool was the first of its kind, as it enables NISAR data processing through an open-source GEE repository, further expanding and improving the utility of NASA Earth observations and contributing to NASA Open Science initiatives. We anticipate the tool will be used by researchers and practitioners interested in wetland monitoring and management..

Lori Berberian↗

Technology Assessment for Producing Propellant From Lunar Water

Introduction: The presence of water ice in permanently shadowed regions (PSRs) on the lunar surface may enable a sustained human presence on the Moon with minimal need for consumables. One of the most valuable uses of lunar water is to produce rocket propellant. A sustained human presence on the Moon will re-quire frequent launches from the lunar surface, and each launch will require tons of propellant. However, many other volatiles are likely to be present in the areas where lunar water is stable1. Producing propellant from lunar water in-situ will require the separation and purification of water before it can be electrolyzed into oxygen and hydrogen gas. The Resource Exploration and Science of OUR Cosmic Environment (RESOURCE) project, funded by the Solar System Exploration Research Virtual Institute (SSERVI) program, includes a task to demonstrate the end-to-end process of water extraction, capture, purification and electrolysis to produce pure/dry oxygen gas sourced from an icy regolith mixture. Water Purification: The requirements for water purity are driven by the type of water electrolyzer used. For example, the water electrolyzer currently used on the International Space Station is based on proton ex-change membrane (PEM) technology. PEM-based electrolyzers require water to be ultrapure and deionized (>10 MegaOhm-cm) so that electrical conductivity is limited to the membranes themselves, where electrical current is used to convert H2O into H2 and O2.. A practical first step toward purifying water that is sourced from a lunar PSR is to use freeze distillation to separate water from other volatiles2. A sub-micronic filter can also be used to ensure that a minimal amount of dust is present in any lunar water that is captured. The combination of freeze distillation and sub-micronic filtration will result in relatively pure water but would require a deionization step if being fed into a PEM electrolyzer. Water Electrolysis: An alternative to PEM electrolysis is to use a Solid Oxide Electrolyzer (SOE). This technology operates at a much higher operating temperature (~800oC) and has a limited pressure range but does not require water to be ultrapure and deionized. Another advantage of SOE technology is that it produces pure dry oxygen gas without the need of an additional drying step. PEM electrolyzers produce humid oxygen gas and the humidity must be removed prior to liquefaction. Assessment: In order to compare PEM and SOE technologies side-by-side, they must be compared at a system level that includes water purification and gas drying steps. Engineers at the Johnson Space Center up-dated and utilized a detailed In-Situ Resource Utilization (ISRU) system model to compare and select the best option for the RESOURCE project which will conclude with a laboratory demonstration of the process of producing pure/dry oxygen from an icy regolith mixture in 2025.

A.J. Paz↗

WET Water Resources: A Google Earth Engine Python API Tool to Automate Wetland Extent Mapping Using Radar Satellite Sensors for Wetland Management and Monitoring

Wetland ecosystems are annually or seasonally wet transition zones between land and water. They provide a range of ecosystem services such as water filtration, flood mitigation, and carbon sequestration, as well as hosting biodiversity hotspots. Although they fulfill fundamental physical and natural processes, wetland extent and health are threatened by anthropogenic influences related to urbanization, population increase, pollution, and climate change. Recognizing the need to quantitatively monitor changes in these recently threatened ecosystems in a timely and cost-effective way, we developed a Google Earth Engine (GEE) Python API tool for automated wetland extent mapping using optical and radar satellite sensors that can be applied globally. The tool will significantly improve wetland change analysis and monitoring as SAR data provides high resolution (5-10 m) imagery, unaffected by cloud cover and light availability (day vs. night), common limitations for other remotely sensed sensors. The tool utilizes Copernicus Sentinel-1 C-band and NISAR L-band (once operational and available on the GEE repository) synthetic aperture radar (SAR) imagery. During image preprocessing, we applied a Terra Moderate Resolution Imaging Spectroradiometer (MODIS) snow product to determine regional snow coverage, which affects land classification sensitivity. Calibration and validation were conducted through a historical change and sensitivity analysis of the Sudd wetland located in central Sudan. The tool was the first of its kind, as it enables NISAR data processing through an open-source GEE repository, further expanding and improving the utility of NASA Earth observations and contributing to NASA Open Science initiatives. We anticipate the tool will be used by researchers and practitioners interested in wetland monitoring and management.

Inundation↗

Space Algae-2: Preflight Testing for A Long-Duration, Multi-Omics Analysis of Arthrospira Platensis

The cyanobacteria Arthrospira platensis NIES-39, commonly known as spirulina, could provide a fresh supply of nutrients for crew on long-duration spaceflight missions. Spirulina is a readily digestible food that is high in protein with all essential amino acids as well as significant levels of B vitamins, antioxidants, and anti-inflammatory metabolites. Spaceflight has multiple abiotic stressors such as increased ionizing radiation and microgravity, which causes a lack of convective mixing. These environmental conditions may impact productivity, nutritional composition, and in long-duration propagation, spaceflight stress may impact the genetic stability of spirulina cultures. We are developing an International Space Station experiment to continuously culture A. platensis for six months. Multi-omics profiling will be used to monitor for changes in the genome, transcriptome, proteome, and metabolome to determine if A. platensis is a suitable nutritional supplement on long-duration missions. During preflight testing we developed a protocol for inoculated liquid cultures to survive a 10-week storage period prior to photo-incubation. The bioreactor bag, temperature, and lighting conditions that support a 14-day growth cycle between passages were also determined. Media testing identified minimal salts supporting robust growth that can be stored in liquid or dry form. A simple filtration method was developed to dewater cultures and harvest biomass for frozen sample return. We optimized a cryopreservation method to enable return of live cells for isolation of individual A. platensis clones. The concept of operations for Space Algae-2 developed from these test results as well as progress on multi-omics analysis methods will be presented.

Algae↗

Technology Assessment for Producing Propellant From Lunar Water

Introduction: The presence of water ice in permanently shadowed regions (PSRs) on the lunar surface may enable a sustained human presence on the Moon with minimal need for consumables. One of the most valuable uses of lunar water is to produce rocket propellant. A sustained human presence on the Moon will re-quire frequent launches from the lunar surface, and each launch will require tons of propellant. However, many other volatiles are likely to be present in the areas where lunar water is stable1. Producing propellant from lunar water in-situ will require the separation and purification of water before it can be electrolyzed into oxygen and hydrogen gas. The Resource Exploration and Science of OUR Cosmic Environment (RESOURCE) project, funded by the Solar System Exploration Research Virtual Institute (SSERVI) program, includes a task to demonstrate the end-to-end process of water extraction, capture, purification and electrolysis to produce pure/dry oxygen gas sourced from an icy regolith mixture. Water Purification: The requirements for water purity are driven by the type of water electrolyzer used. For example, the water electrolyzer currently used on the International Space Station is based on proton ex-change membrane (PEM) technology. PEM-based electrolyzers require water to be ultrapure and deionized (>10 MegaOhm-cm) so that electrical conductivity is limited to the membranes themselves, where electrical current is used to convert H2O into H2 and O2.. A practical first step toward purifying water that is sourced from a lunar PSR is to use freeze distillation to separate water from other volatiles2. A sub-micronic filter can also be used to ensure that a minimal amount of dust is present in any lunar water that is captured. The combination of freeze distillation and sub-micronic filtration will result in relatively pure water but would require a deionization step if being fed into a PEM electrolyzer. Water Electrolysis: An alternative to PEM electrolysis is to use a Solid Oxide Electrolyzer (SOE). This technology operates at a much higher operating temperature (~800oC) and has a limited pressure range but does not require water to be ultrapure and deionized. Another advantage of SOE technology is that it produces pure dry oxygen gas without the need of an additional drying step. PEM electrolyzers produce humid oxygen gas and the humidity must be removed prior to liquefaction. Assessment: In order to compare PEM and SOE technologies side-by-side, they must be compared at a system level that includes water purification and gas drying steps. Engineers at the Johnson Space Center up-dated and utilized a detailed In-Situ Resource Utilization (ISRU) system model to compare and select the best option for the RESOURCE project which will conclude with a laboratory demonstration of the process of producing pure/dry oxygen from an icy regolith mixture in 2025.

ISRU↗

The Influence of Smoke Particle Properties and Cabin Characteristics on Smoke Detection in Lunar Gravity

Spacecraft fires pose a threat to the success of future Lunar, Martian, and deep space exploration missions. As NASA plans to return humans to the Moon in the next decade, novel mission requirements will present new fire safety challenges. For example, materials that are fire resistant on Earth are expected to burn under planned habitat conditions (elevated oxygen concentrations and reduced cabin pressure) and partial gravity (0.16g). Optimal smoke detector placement will depend on a combination of buoyant plume velocities, induced by partial gravity, and Environmental Control and Life Support Systems (ECLSS) parameters, including particle filtration rates, supply and return placement within the cabin, and forced air velocities. These ECLSS parameters must also address the need for rapid Lunar dust removal, as Lunar dust exposure poses a risk to crew health and hardware functionality. Here, we present progress toward a computational fluid dynamics model to evaluate smoke transport in a Lunar habitat. Recent work has demonstrated that if air supplies are placed on ceilings and returns on the floor, a buoyant smoke layer at the ceiling may disperse over the order of minutes even under low forced flow conditions. We expand upon these results to examine the influence of different supply and return configurations on smoke plume development. Additionally, we also address differences in the transport of smoke particles and Lunar dust by varying particle parameters like size, density, and shape factor. Finally, we discuss ongoing and future experimental efforts to measure smoke particle properties and transport under partial gravity, elevated oxygen, and reduced pressure conditions.

Claire Fortenberry↗

High-Pressure Oxygen Dryer (HPOD) FY 2023 Trade Study and Market Survey

A reliable source of dry, high-pressure oxygen is needed for future lunar missions to support nominal and contingency operations on the Surface Habitat (SH) and the Pressurized Rover (PR) in future Gateway missions. A High-Pressure Oxygen Dryer (HPOD) is needed to support potential use of primary oxygen generation systems, the High-Pressure Oxygen Generation Assembly (HPOGA) and the Skyre EOC, by drying the wet oxygen exiting the systems. The objective of this paper is to perform an architecture trade study and market survey of options to dry a continuous stream of 45°C, 4000 PSIG oxygen containing ~ 500 ppm H2O (dew point 52°C) down to < 7 ppm H2O (dew point -15°C) for both the 28-day and 365-day timeframe. A total of seven different technologies were identified as potential dryer candidates during the market survey, relying on one of three principles, adsorption, filtration, and cooling. During the sizing and trade study, it was found that several of the technologies aren’t sufficient for the extreme conditions and were eliminated. In the end, a single-use throw away adsorption bed traded the best for single mission use, while a pressure regenerative adsorption bed traded the best for a multiple mission scenario. The results of this analysis are intended to influence future design work for high-pressure oxygen drying applications.

High Pressure Oxygen↗

Modeling Evolvable Water Recovery Systems for Short and Long-Duration Missions in Partial Gravity

Water recovery technologies on the International Space Station (ISS) are designed and optimized for microgravity environments, thus creating a need for innovative systems optimized for mission operations in the presence of gravity. Alternative water recovery technologies under research by the Life Support Systems division were compiled into a series of Partial Gravity Water Recovery System (PGWRS) architectures. Individual unit processes were modeled via fundamental physical-chemical process equations to simulate their method of treatment, including chemical or biological oxidation, flash evaporation, filtration, or a combination of adsorption and ion exchange processes. Dimension and sizing data were then utilized to scale each architecture. Modeling was completed on an assortment of architectures assuming three scenarios in which treatment methods could take advantage of partial gravity: a short-duration mission with a temporary surface habitat, a longer duration mission with a more permanent habitat, and a long duration mission without resupply. Total system mass was estimated to quantitatively compare architectures within scenarios, but qualitative observations were also made regarding system robustness, flexibility, and capacity to meet more stringent demands. Technologies that traded most favorably between the scenarios were those that included a pre-oxidation stage to minimize mass loading to downstream absorption and ion exchange beds, exploited gravity in liquid-vapor separation processes, and reconstituted wastewater components into recyclable material streams.

Avery Carlson↗

Space Algae-2: Preflight Testing for A Long-Duration, Multi-Omics Analysis of Arthrospira Platensis

The cyanobacteria Arthrospira platensis NIES-39, commonly known as spirulina, could provide a fresh supply of nutrients for crew on long-duration spaceflight missions. Spirulina is a readily digestible food that is high in protein with all essential amino acids as well as significant levels of B vitamins, antioxidants, and anti-inflammatory metabolites. Spaceflight has multiple abiotic stressors such as increased ionizing radiation and microgravity, which causes a lack of convective mixing. These environmental conditions may impact productivity, nutritional composition, and in long-duration propagation, spaceflight stress may impact the genetic stability of spirulina cultures. We are developing an International Space Station experiment to continuously culture A. platensis for six months. Multi-omics profiling will be used to monitor for changes in the genome, transcriptome, proteome, and metabolome to determine if A. platensis is a suitable nutritional supplement on long-duration missions. During preflight testing we developed a protocol for inoculated liquid cultures to survive a 10-week storage period prior to photo-incubation. The bioreactor bag, temperature, and lighting conditions that support a 14-day growth cycle between passages were also determined. Media testing identified minimal salts supporting robust growth that can be stored in liquid or dry form. A simple filtration method was developed to dewater cultures and harvest biomass for frozen sample return. We optimized a cryopreservation method to enable return of live cells for isolation of individual A. platensis clones. The concept of operations for Space Algae-2 developed from these test results as well as progress on multi-omics analysis methods will be presented.

Algae↗