Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “target variability”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,459 records · Page 81

Foundations of Feature Selection and Classification for Non-Gaussian Distributed Targets

This report describes the author’s research toward creating algorithms for selecting useful statistical features in pattern/target classification problems in which the features are non-Gaussian distributed. In engineering practice, it is common to either (1) Not perform any feature selection procedure, or (2) Use a feature selection algorithm that assumes the features are Gaussian distributed. This process can be far from optimal if the data are actually non-Gaussian, as they frequently are in practice. This research has the goal of mitigating that problem by creating a useful algorithm that can be used in practice. The approach is to focus on the performance indices used in common feature selection algorithms, such as Branch and Bound algorithms and the Sequential Forward Selection Algorithm. Ordinarily, the performance index used to measure the class separation in feature space involves assuming the data are Gaussian and deriving tractable performance indices that use closed form equations that represent the probability density functions of the class data. The advantage of this approach is that it produces feature selection algorithms that have low computational complexity and do not require knowledge of the actual data densities. The disadvantage is that these algorithms may not perform reasonably when the data are non-Gaussian, as they commonly are in practice. This research examines the use of information-theoretic class separability measures that can deal with the non-Gaussian case. In particular, this work shows that the Hellinger Distance (a divergence measure), has very desirable mathematical properties and can be useful for feature selection. The work in this paper employs a multivariate kernel density estimator along with the Hellinger Distance and an optimal subset selection algorithm to do feature selection. This report (1) Defines the classification and feature selection problems, (2) Describes the continuous variable Hellinger distance in one dimension and discusses its mathematical properties in comparison with other distance measures, (3) Develops the discrete variable Hellinger distance expression for one and two dimensions, and shows that the discrete Hellinger distance for multiple dimensions is a straightforward extension, and (4) Presents experimental density estimation results using a new MATLAB implementation of a multivariate kernel density estimator. The draft of this report served as a starting point for Ensign Matthew Wilder’s MSEE thesis work at the Naval Postgraduate School (NPS) in 2010 and 2011. Ensign Wilder completed his thesis and graduated from NPS in June 2011 (M. J. Wilder, “Automatic Target Recognition: Statistical Feature Selection of Non-Gaussian Distributed Target Classes,” MSEE Thesis, Naval Postgraduate School, Thesis Advisor: Grace A. Clark, Second Reader: Monique P. Fargues, June 2011). In the thesis, Ensign Wilder created implementations of the Branch and Bound and Sequential Feature Selection algorithms, extended the multivariate kernel density estimator code, and evaluated the performance of Hellinger Distance-based feature selection algorithms using both simulated data and data from a real-world benchmark data set. The new algorithm was shown to be very effective for both Gaussian and non-Gaussian data sets. The advantages gained for non-Gaussian data come at the cost of increased computational complexity. Using a laptop computer for practical problems, we generally use initial feature vectors containing no more than about ten or twelve features. Proposed future research lies in creating faster kernel density estimator algorithms that would allow for the use of higher dimensional data.

42 ENGINEERING↗

Preliminary characterization of a laser-generated plasma sheet

We present the results from recent experiments to create a flowing plasma sheet. Two groups of three laser beams with nominally 1.5 kJ of energy per group were focused to separate pointing locations, driving a shock into a wedge target. As the shock breaks out of the wedge, the plasma is focused on center, creating a sheet of plasma. Measurements at 60 ns indicate the plasma sheet has propagated 2825 microns with an average velocity of 49 microns/ns. These experiments follow previous experiments, which are aimed at studying similar physics as that found in the hot spot region of cataclysmic variables. Krauland et al created a flowing plasma, which represents the flowing plasma from the secondary star. This flow interacted with a stationary object, which represented the disk around the white dwarf. A reverse shock is a shock formed when a freely expanding plasma encounters an obstacle. Reverse shocks can be generated by a blast wave propagating through a medium. As a result, they can also be found in binary star systems where the flowing gas from a companion star interacts with the accretion disk of the primary star.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Development of a Fluorescence-Activated Cell Sorting Method Coupled with Whole Genome Amplification To Analyze Minority and Trace Dehalococcoides Genomes in Microbial Communities

Dehalococcoides mccartyi are functionally important bacteria that catalyze the reductive dechlorination of chlorinated ethenes. However, these anaerobic bacteria are fastidious to isolate, making downstream genomic characterization challenging. In order to facilitate genomic analysis, a fluorescence-activated cell sorting (FACS) method was developed in this study to separate D. mccartyi cells from a microbial community, and the DNA of the isolated cells was processed by whole genome amplification (WGA) and hybridized onto a D. mccartyi microarray for comparative genomics against four sequenced strains. First, FACS was successfully applied to a D. mccartyi isolate as positive control, and then microarray results verified that WGA from 10 6 cells or ∼1 ng of genomic DNA yielded high-quality coverage detecting nearly all genes across the genome. As expected, some inter- and intrasample variability in WGA was observed, but these biases were minimized by performing multiple parallel amplifications. Subsequent application of the FACS and WGA protocols to two enrichment cultures containing ∼10% and ∼1% D. mccartyi cells successfully enabled genomic analysis. As proof of concept, this study demonstrates that coupling FACS with WGA and microarrays is a promising tool to expedite genomic characterization of target strains in environmental communities where the relative concentrations are low.

59 BASIC BIOLOGICAL SCIENCES↗

Advanced manufacturing provides tailor-made solutions for crystallography with x-ray free-electron lasers

Serial crystallography at large facilities, such as x-ray free-electron lasers and synchrotrons, evolved as a powerful method for the high-resolution structural investigation of proteins that are critical for human health, thus advancing drug discovery and novel therapies. However, a critical barrier to successful serial crystallography experiments lies in the efficient handling of the protein microcrystals and solutions at microscales. Microfluidics are the obvious approach for any high-throughput, nano-to-microliter sample handling, that also requires design flexibility and rapid prototyping to deal with the variable shapes, sizes, and density of crystals. Here, we discuss recent advances in polymer 3D printing for microfluidics-based serial crystallography research and present a demonstration of emerging, large-scale, nano-3D printing approaches leading into the future of 3D sample environment and delivery device fabrication from liquid jet gas-dynamic virtual nozzles devices to fixed-target sample environment technology.

36 MATERIALS SCIENCE↗

Computational Estimation by Scientific Data Mining with Classical Methods to Automate Learning Strategies of Scientists

Experimental results are often plotted as 2-dimensional graphical plots (aka graphs) in scientific domains depicting dependent versus independent variables to aid visual analysis of processes. Repeatedly performing laboratory experiments consumes significant time and resources, motivating the need for computational estimation. The goals are to estimate the graph obtained in an experiment given its input conditions, and to estimate the conditions that would lead to a desired graph. Existing estimation approaches often do not meet accuracy and efficiency needs of targeted applications. We develop a computational estimation approach called AutoDomainMine that integrates clustering and classification over complex scientific data in a framework so as to automate classical learning methods of scientists. Knowledge discovered thereby from a database of existing experiments serves as the basis for estimation. Challenges include preserving domain semantics in clustering, finding matching strategies in classification, striking a good balance between elaboration and conciseness while displaying estimation results based on needs of targeted users, and deriving objective measures to capture subjective user interests. These and other challenges are addressed in this work. The AutoDomainMine approach is used to build a computational estimation system, rigorously evaluated with real data in Materials Science. Our evaluation confirms that AutoDomainMine provides desired accuracy and efficiency in computational estimation. It is extendable to other science and engineering domains as proved by adaptation of its sub-processes within fields such as Bioinformatics and Nanotechnology.

Computer Science↗

QProR: An Efficient Framework for Quantity-of-Interest Based Progressive Retrieval with Guaranteed Error Control

Scientific applications generate an unprecedented volume of data, overwhelming the network and file systems’ bandwidth and posing challenges for efficient and scalable data retrieval and analysis. Progressive data compression offers a promising solution by enabling on-demand retrieval at reduced size. However, existing progressive methods either fail to bound the errors in essential quantities of interest (QoIs) derived from raw data or suffer from suboptimal retrieval efficiency. In this work, we propose QProR, an efficient QoI-based progressive framework that optimizes progressive retrieval for target QoIs. Our key contributions include: (1) a systematic framework that integrates error-controlled lossy compressors with bitplane encoding while decoupling the two processes for high flexibility and adaptability; (2) a novel weighted bitplane encoding method which incorperates QoI knowledge into data refactoring to enhance retrieval efficiency; (3) an optimized retrieval strategy that accounts for the varying impacts of different variables on multivariate QoIs; (4) comprehensive evaluations using six real-world datasets from multiple scientific applications and thorough comparisons against state of the arts. Experimental results demonstrate that QProR achieves up to 80.38% reduction in the retrieval size under the same requested QoI error tolerance, when compared with the best-performing existing methods. When transferring 384 GB of scientific data to remote sites, QProR delivers up to 1.68 × speedup in the end-to-end data transfer performance.

Li, Wenbo [University of Kentucky]↗

Phased Retrofits in Existing Homes In Florida Phase I: Shallow and Deep Retrofits

The U.S. Department of Energy (DOE) Building America program, in collaboration with Florida Power and Light (FPL), conducted a phased residential energy-efficiency retrofit program. This research sought to establish impacts on annual energy and peak energy reductions from the technologies applied at two levels of retrofit - shallow and deep, with savings levels approaching the Building America program goals of reducing whole-house energy use by 40%. Under the Phased Deep Retrofit (PDR) project, we have installed phased, energy-efficiency retrofits in a sample of 56 existing, all-electric homes. End-use savings and economic evaluation results from the phased measure packages and single measures are summarized in this report. Project results will be of interest to utility program designers, weatherization evaluators, and the housing remodel industry. Shallow retrofits were conducted in all homes from March to June 2013. The measures for this phase were chosen based on ease of installation, targeting lighting (CFLs and LED lamps), domestic hot water (wraps and showerheads), refrigeration (cleaning of coils), pool pump (reduction of operating hours), and the home entertainment center (smart plugs). Deep retrofits were conducted on a subset of ten PDR homes from May 2013 through March 2014. Measures included new air source heat pumps, duct repair, ceiling insulation, heat pump water heaters, variable speed pool pumps and learning thermostats. Major appliances such as refrigerators and dishwashers were replaced where they were old and inefficient.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Development of a comprehensive software engineering environment

The generation of a set of tools for software lifecycle is a recurring theme in the software engineering literature. The development of such tools and their integration into a software development environment is a difficult task because of the magnitude (number of variables) and the complexity (combinatorics) of the software lifecycle process. An initial development of a global approach was initiated in 1982 as the Software Development Workbench (SDW). Continuing efforts focus on tool development, tool integration, human interfacing, data dictionaries, and testing algorithms. Current efforts are emphasizing natural language interfaces, expert system software development associates and distributed environments with Ada as the target language. The current implementation of the SDW is on a VAX-11/780. Other software development tools are being networked through engineering workstations.

Hartrum, Thomas C.↗

The use of designed experiments in the process development of continuous propellant mixing

A continuous mix pilot plant was constructed at Aerojet Propulsion Division in Sacramento, California to develop a robust propellant mixing process for the full scale plant that was to be built at the NASA Advanced Solid Rocket Motor facility Yellow Creek, Mississippi. The plant was used to conduct dozens of subsystem and full system mixing tests for evaluation of equipment, processing methods, and control schemes for later use at the production plant. As a culmination to this work, a series of designed experiments were conducted using an eight run Taguchi analysis with four factors at two levels each to determine the primary effect of processing parameters on propellant ballistic and mechanical properties. The factors examined in these runs included the propellant production rate (454 (1000) and 622 kg/hr (1371 Ib/hr)), the product temperature out of the mixer (49 (120) and 63 deg C (145 deg F)), mixer screw speed (75 and 90 rpm), and the deaerator excess capacity (20 and 80 percent). Measured response variables included the uncured and cured density, Crawford Bomb liquid strand burning rates, and selected mechanical properties. The experiment revealed that several of the response variables displayed significant changes from run-to-run with the product temperature being the single most important factor. After concluding this experiment, a twenty-six hour confirmation run was conducted to verify the conclusions reached in the designed experiment. The extended run produced over 12,250 kgs (27,000 lbs) of propellant meeting all of the pre-run targeted properties including density (1.803 g/cc (0.065 lb/in(exp 3)) with a 0.12 percent coefficient of variation (CV) at 25 deg C (77 deg F)), liquid strand burn rate (0.889 cm/s (0.350 in/s) with a 0.69 percent CV at 4210 KPa (610 psig), 15.6 deg C (60 deg F)), nominal maximum stress (828 KPa (120 psig) with a 2.84 percent CV, S&E at 25 deg C (77 deg F), 5.08 cm/min (2 in/min)), strain at nominal maximum (47.4 percent with a 3.96 percent CV), and initial tangent modulus (5349 KPa (775 psig) with a 7.26 percent CV).

Campbell, J. A.↗

Employing Sensitivity Derivatives for Robust Optimization under Uncertainty in CFD

A robust optimization is demonstrated on a two-dimensional inviscid airfoil problem in subsonic flow. Given uncertainties in statistically independent, random, normally distributed flow parameters (input variables), an approximate first-order statistical moment method is employed to represent the Computational Fluid Dynamics (CFD) code outputs as expected values with variances. These output quantities are used to form the objective function and constraints. The constraints are cast in probabilistic terms; that is, the probability that a constraint is satisfied is greater than or equal to some desired target probability. Gradient-based robust optimization of this stochastic problem is accomplished through use of both first and second-order sensitivity derivatives. For each robust optimization, the effect of increasing both input standard deviations and target probability of constraint satisfaction are demonstrated. This method provides a means for incorporating uncertainty when considering small deviations from input mean values.

Newman, Perry A.↗

The Time Series Technique for Aerosol Retrievals over Land from MODIS: Algorithm MAIAC

Atmospheric aerosols interact with sun light by scattering and absorbing radiation. By changing irradiance of the Earth surface, modifying cloud fractional cover and microphysical properties and a number of other mechanisms, they affect the energy balance, hydrological cycle, and planetary climate [IPCC, 2007]. In many world regions there is a growing impact of aerosols on air quality and human health. The Earth Observing System [NASA, 1999] initiated high quality global Earth observations and operational aerosol retrievals over land. With the wide swath (2300 km) of MODIS instrument, the MODIS Dark Target algorithm [Kaufman et al., 1997; Remer et al., 2005; Levy et al., 2007] currently complemented with the Deep Blue method [Hsu et al., 2004] provides daily global view of planetary atmospheric aerosol. The MISR algorithm [Martonchik et al., 1998; Diner et al., 2005] makes high quality aerosol retrievals in 300 km swaths covering the globe in 8 days. With MODIS aerosol program being very successful, there are still several unresolved issues in the retrieval algorithms. The current processing is pixel-based and relies on a single-orbit data. Such an approach produces a single measurement for every pixel characterized by two main unknowns, aerosol optical thickness (AOT) and surface reflectance (SR). This lack of information constitutes a fundamental problem of the remote sensing which cannot be resolved without a priori information. For example, MODIS Dark Target algorithm makes spectral assumptions about surface reflectance, whereas the Deep Blue method uses ancillary global database of surface reflectance composed from minimal monthly measurements with Rayleigh correction. Both algorithms use Lambertian surface model. The surface-related assumptions in the aerosol retrievals may affect subsequent atmospheric correction in unintended way. For example, the Dark Target algorithm uses an empirical relationship to predict SR in the Blue (B3) and Red (B1) bands from the 2.1 m channel (B7) for the purpose of aerosol retrieval. Obviously, the subsequent atmospheric correction will produce the same SR in the red and blue bands as predicted, i.e. an empirical function of 2.1. In other words, the spectral, spatial and temporal variability of surface reflectance in the Blue and Red bands appears borrowed from band B7. This may have certain implications for the vegetation and global carbon analysis because the chlorophyll-sensing bands B1, B3 are effectively substituted in terms of variability by band B7, which is sensitive to the plant liquid water. This chapter describes a new recently developed generic aerosol-surface retrieval algorithm for MODIS. The Multi-Angle Implementation of Atmospheric Correction (MAIAC) algorithm simultaneously retrieves AOT and surface bi-directional reflection factor (BRF) using the time series of MODIS measurements.

Lyapustin, Alexei↗

Framework for Multidisciplinary Analysis, Design, and Optimization with High-Fidelity Analysis Tools

A plan is presented for the development of a high fidelity multidisciplinary optimization process for rotorcraft. The plan formulates individual disciplinary design problems, identifies practical high-fidelity tools and processes that can be incorporated in an automated optimization environment, and establishes statements of the multidisciplinary design problem including objectives, constraints, design variables, and cross-disciplinary dependencies. Five key disciplinary areas are selected in the development plan. These are rotor aerodynamics, rotor structures and dynamics, fuselage aerodynamics, fuselage structures, and propulsion / drive system. Flying qualities and noise are included as ancillary areas. Consistency across engineering disciplines is maintained with a central geometry engine that supports all multidisciplinary analysis. The multidisciplinary optimization process targets the preliminary design cycle where gross elements of the helicopter have been defined. These might include number of rotors and rotor configuration (tandem, coaxial, etc.). It is at this stage that sufficient configuration information is defined to perform high-fidelity analysis. At the same time there is enough design freedom to influence a design. The rotorcraft multidisciplinary optimization tool is built and substantiated throughout its development cycle in a staged approach by incorporating disciplines sequentially.

Orr, Stanley A.↗

Heliophysics 2009 Roadmap and Global Change: Possibilities for Improved Understanding of the Connection

Heliophysics is the science that includes all aspects of the research needed to understand the Sun and its effects on the Earth and the solar system. Six science targets: 1. Origins of Near-Earth Plasma - to understand the origin and transport of terrestrial plasma from its source to the magnetosphere and solar wind. 2. Solar Energetic Particle Acceleration and Transport - to understand how and where solar eruptions accelerate energetic particles that reach Earth. 3. Ion-Neutral Coupling in the Atmosphere - to understand how neutral winds control ionospheric variability. 4. Climate Impacts of Space Radiation - to understand our atmosphere s response to auroral, radiation belt, and solar energetic particles, and the associated effects on nitric oxide (NO) and ozone. 5. Dynamic Geospace Coupling - to understand how magnetospheric dynamics provides energy into the coupled ionosphere-magnetosphere system. 6. Heliospheric Magnetics - to understand the flow and dynamics of transient magnetic structures form the solar interior to Earth.

Spann, Jim↗

The Kepler Data Processing Handbook: A Field Guide to Prospecting for Habitable Worlds

The Kepler telescope hurtled into orbit in March 2009, initiating NASA's first mission to discover Earth-size planets orbiting Sun-like stars. Kepler simultaneously collected data for approximately 165,000 target stars at a time over its four-year mission, identifying over 4700 planet candidates, over 2300 confirmed or validated planets, and over 2100 eclipsing binaries. While Kepler was designed to discover exoplanets, the long-term, ultrahigh photometric precision measurements it achieved made it a premier observational facility for stellar astrophysics, especially in the field of asteroseismology, and for variable stars, such as RR Lyrae. The Kepler Science Operations Center (SOC) was developed at NASA Ames Research Center to process the data acquired by Kepler from pixel-level calibrations all the way to identifying transiting planet signatures and subjecting them to a suite of diagnostic tests to establish or break confidence in their planetary nature. Detecting small, rocky planets transiting Sun-like stars presents a variety of daunting challenges, including achieving an unprecedented photometric precision of ~20 ppm on 6.5-hour timescales, and supporting the science operations, management, processing, and repeated reprocessing of the accumulating data stream. A newly revised and expanded version of the Kepler Data Processing Handbook (KDPH) has been released to support the legacy archival products. The KDPH details the theory, design and performance of the algorithms supporting each data processing step. This paper presents an overview of the KDPH and features illustrations of several key algorithms in the Kepler Science Data Processing Pipeline. Kepler was selected as the 10th mission of the Discovery Program. Funding for this mission is provided by NASA, Science Mission Directorate.

high performance computing↗

Welding in Space: Past, Present, and Future

It has been over fifty years since the first welds were made in space by Soviet cosmonauts on Soyuz-6 in October of 1969. The United States performed bead-on-plate welding, brazing, and metal melting experiments onboard the Skylab orbital space station several years later in 1973. Finally, Soviet cosmonauts departed their Salyut-7 capsule and made the first (and last) welds in open space in 1984. Progress on further demonstrations of welding in space stagnated, and subsequent microgravity welding research work shifted to lower-cost earth-based experiments that include drop towers and parabolic flights. With the advent of the International Space Station, relevant microgravity research was undertaken in the field of metal solidification science, and limited studies of brazing and soldering were undertaken; however, no welds have ever been on the Space Station. Therefore, it has been almost 40 years since a weld was made in space. Much like terrestrial construction and manufacturing industries, welding, joining, and allied processes will be enabling technologies for In-space Assembly and Manufacturing (ISAM) in the nascent “Space Economy”, a sector that is expected to approach a value of 10^12 USD within the next decade. It is critical that the welding and joining research community, along with the welding industry, engages the space industry to advance the understanding of those critical manufacturing processes which must be evaluated and matured in the extreme environments of space. Such environments include variable gravity (microgravity in low earth orbit, 0.17 gravity on the lunar surface, and 0.38 gravity on the martian surface), reduced pressure (extreme vacuum in space and the lunar surface to a predominantly CO2 atmosphere on Mars), and extreme temperatures (between 40 and 400 K). Past experiments and analyses are reviewed to suggest requirements that the welding and joining community should target to make strides on closing the current space welding gaps. Efforts to continue welding in space are currently underway at NASA in conjunction with academia and industry. Those are explored along with future agency goals which are seen as opportunities to engage the welding community on this historic effort.

in-space assembly and manufacturing↗

Dynamic electrophoretic fingerprinting of the HIV-1 envelope glycoprotein

Background: Interactions between the HIV-1 envelope glycoprotein (Env) and its primary receptor CD4 are influenced by the physiological setting in which these events take place. In this study, we explored the surface chemistry of HIV-1 Env constructs at a range of pH and salinities relevant to mucosal and systemic compartments through electrophoretic mobility (EM) measurements. Sexual transmission events provide a more acidic environment for HIV-1 compared to dissemination and spread of infection occurring in blood or lymph node. We hypothesize functional, trimeric Env behaves differently than monomeric forms. Results: The dynamic electrophoretic fingerprint of trimeric gp140 revealed a change in EM from strongly negative to strongly positive as pH increased from that of the lower female genital tract (pHx) to that of the blood (pHy). Similar findings were observed using a trimeric influenza Haemagglutinin (HA) glycoprotein, indicating that this may be a general attribute of trimeric viral envelope glycoproteins. These findings were supported by computationally modeling the surface charge of various gp120 and HA crystal structures. To identify the behavior of the infectious agent and its target cells, EM measurements were made on purified whole HIV-1 virions and primary T-lymphocytes. Viral particles had a largely negative surface charge, and lacked the regions of positivity near neutral pH that were observed with trimeric Env. T cells changed their surface chemistry as a function of activation state, becoming more negative over a wider range of pH after activation. Soluble recombinant CD4 (sCD4) was found to be positively charged under a wide range of conditions. Binding studies between sCD4 and gp140 show that the affinity of CD4-gp140 interactions depends on pH. Conclusions: Taken together, these findings allow a more complete model of the electrochemical forces involved in HIV-1 Env functionality. These results indicate that the influence of the localized environment on the interactions of HIV with target cells are more pronounced than previously appreciated. There is differential chemistry of trimeric, but not monomeric, Env under conditions which mimic the mucosa compared to those found systemically. This should be taken into consideration during design of immunogens which targets virus at mucosal portals of entry.

59 BASIC BIOLOGICAL SCIENCES↗

High-Grade Glioma Radiation Therapy Target Volumes and Patterns of Failure Obtained From Magnetic Resonance Imaging and {sup 18}F-FDOPA Positron Emission Tomography Delineations From Multiple Observers

Purpose: The objective of this study was to compare recurrent tumor locations after radiation therapy with pretreatment delineations of high-grade gliomas from magnetic resonance imaging (MRI) and 3,4-dihydroxy-6-[{sup 18}F]fluoro-L-phenylalanine ({sup 18}F-FDOPA) positron emission tomography (PET) using contours delineated by multiple observers. Methods and Materials: Nineteen patients with newly diagnosed high-grade gliomas underwent computed tomography (CT), gadolinium contrast-enhanced MRI, and {sup 18}F-FDOPA PET/CT. The image sets (CT, MRI, and PET/CT) were registered, and 5 observers contoured gross tumor volumes (GTVs) using MRI and PET. Consensus contours were obtained by simultaneous truth and performance level estimation (STAPLE). Interobserver variability was quantified by the percentage of volume overlap. Recurrent tumor locations after radiation therapy were contoured by each observer using CT or MRI. Consensus recurrence contours were obtained with STAPLE. Results: The mean interobserver volume overlap for PET GTVs (42% ± 22%) and MRI GTVs (41% ± 22%) was not significantly different (P=.67). The mean consensus volume was significantly larger for PET GTVs (58.6 ± 52.4 cm{sup 3}) than for MRI GTVs (30.8 ± 26.0 cm{sup 3}, P=.003). More than 95% of the consensus recurrence volume was within the 95% isodose surface for 11 of 12 (92%) cases with recurrent tumor imaging. Ten (91%) of these cases extended beyond the PET GTV, and 9 (82%) were contained within a 2-cm margin on the MRI GTV. One recurrence (8%) was located outside the 95% isodose surface. Conclusions: High-grade glioma contours obtained with {sup 18}F-FDOPA PET had similar interobserver agreement to volumes obtained with MRI. Although PET-based consensus target volumes were larger than MRI-based volumes, treatment planning using PET-based volumes may not have yielded better treatment outcomes, given that all but 1 recurrence extended beyond the PET GTV and most were contained by a 2-cm margin on the MRI GTV.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

Enzalutamide response in a panel of prostate cancer cell lines reveals a role for glucocorticoid receptor in enzalutamide resistant disease

Representative in vitro model systems that accurately model response to therapy and allow the identification of new targets are important for improving our treatment of prostate cancer. Here we describe molecular characterization and drug testing in a panel of 20 prostate cancer cell lines. The cell lines cluster into distinct subsets based on RNA expression, which is largely driven by functional Androgen Receptor (AR) expression. KLK3 , the AR-responsive gene that encodes prostate specific antigen, shows the greatest variability in expression across the cell line panel. Other common prostate cancer associated genes such as TMPRSS2 and ERG show similar expression patterns. Copy number analysis demonstrates that many of the most commonly gained (including regions containing TERC and MYC ) and lost regions (including regions containing TP53 and PTEN) that were identified in patient samples by the TCGA are mirrored in the prostate cancer cell lines. Assessment of response to the anti-androgen enzalutamide shows a distinct separation of responders and non-responders, predominantly related to status of wild-type AR. Surprisingly, several AR-null lines responded to enzalutamide. These AR-null, enzalutamide-responsive cells were characterized by high levels of expression of glucocorticoid receptor (GR) encoded by NR3C1 . Treatment of these cells with the anti-GR agent mifepristone showed that they were more sensitive to this drug than enzalutamide, as were several of the enzalutamide non-responsive lines. This is consistent with several recent reports that suggest that GR expression is an alternative signaling mechanism that can bypass AR blockade. This study reinforces the utility of large cell line panels for the study of cancer and identifies several cell lines that represent ideal models to study AR-null cells that have upregulated GR to sustain growth.

59 BASIC BIOLOGICAL SCIENCES↗