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At least 1,459 records · Page 81

Hanford Site Post-Fire Vegetation Monitoring Report for Calendar Year 2021

The Hanford Site presents an expanse of shrub-steppe habitat that provides exceptional value to plants and animals located on the Site and in the surrounding greater Columbia Basin. The greatest threat to this habitat is fire-related conversion to a cheatgrass-dominated monoculture. In order to decrease future fire risk and to disrupt the positive feedback cycle between cheatgrass and fire, land managers can make efforts to restore native vegetation following a fire. The goal of this report is to analyze vegetation recovery post-fire in both restored and unrestored areas to inform future post-fire response actions.

54 ENVIRONMENTAL SCIENCES↗

Hanford Site Rare Plant Monitoring Report for Calendar Year 2023-2024

This report summarizes rare plant monitoring data collected in calendar years (CY) 2023 through CY 2024 and provides management recommendations accordingly. DOE/RL-2021-35, Central Hanford Rare Plant Management Plan, guides the approach to rare plant monitoring and management at the Hanford Site. In CYs 2023 and 2024, rare plant monitoring efforts occurred on the portion of the Hanford Site managed by the Hanford Field Office (HFO; Figure 1-1), referred to herein as Central Hanford. The goal of monitoring is to collect data to evaluate the conservation status of rare plant species. Surveys conducted in CY 2023 through CY 2024 built on previous monitoring efforts, tracking the abundance and distribution of rare plants at Central Hanford. Results from previous monitoring efforts are included for reference. Monitoring data are submitted to the Washington Natural Heritage Program (WNHP) to evaluate statewide conservation statuses.

54 ENVIRONMENTAL SCIENCES↗

Hanford Site Rare Plant Monitoring Report for Calendar Year 2022

Rare plants are monitored on the Hanford Site as distribution and abundance data of current and historic rare plant populations are necessary to make well-informed environmental and ecological resource management decisions. The Central Hanford Rare Plant Management Plan (DOE/RL-2021-35) guides rare plant monitoring methodology in the context of resource management at the Hanford Site. In 2022, a rare plant monitoring effort occurred on the portion of the Hanford Site (referred to herein as Central Hanford) managed by the U.S. Department of Energy, Richland Operations Office (DOE-RL; Figure 1). The goal of this effort was to revisit known occurrences of upland rare plant populations on Central Hanford to improve baseline information on locations of rare plant populations on the Hanford Site. Rare upland plants were thoroughly surveyed in 2021 but unusually low precipitation that year limited germination of many upland annual plants. Surveys in 2022 focused on rare upland annual species after the Hanford Site received higher than average spring precipitation that was expected to increase germination. Rainfall from April 2022 to June 2022 was 76% higher than average; however, these months were also colder than average. Winter rainfall from January 2022 to March 2022 was 84% lower than average. The high spring precipitation in 2022 appears to have triggered the germination of some populations of rare upland annuals but the dry winter and cold spring may have inhibited others. Population stability, viability, and threats were evaluated and will be used to inform future management of rare plant species.

54 ENVIRONMENTAL SCIENCES↗

Hanford Site Rare Plant Monitoring Report for Calendar Year 2021

In 2021, a rare plant monitoring effort occurred on the portion of the Hanford Site (referred to herein as Central Hanford) managed by the U.S. Department of Energy Richland Operations Office (DOE-RL; Figure 1). The goal of this effort was to revisit known occurrences of upland rare plant populations on Central Hanford to improve baseline information on locations of rare plant populations on the Hanford Site. Additionally, population stability, viability, and threats were recorded and will be used to inform future management of rare plant species.

54 ENVIRONMENTAL SCIENCES↗

Hanford Site Rare Plant Monitoring Report for Calendar Year 2016

In 2016, a rare plant monitoring effort occurred on the portion of the Hanford Site (referred to herein as Central Hanford) managed by the U.S. Department of Energy (DOE). The goal of this effort was to revisit known occurrences of upland rare plant populations on Central Hanford to improve baseline information on locations of rare plant populations on the Hanford Site. Additionally, population stability, viability, and threats were recorded and will be used to inform future management of rare plant species.

54 ENVIRONMENTAL SCIENCES↗

Hanford Site Rare Plant Monitoring Report for Calendar Year 2016

In 2016, a rare plant monitoring effort occurred on the portion of the Hanford Site (referred to herein as Central Hanford) managed by the U.S. Department of Energy (DOE). The goal of this effort was to revisit known occurrences of upland rare plant populations on Central Hanford to improve baseline information on locations of rare plant populations on the Hanford Site. Additionally, population stability, viability, and threats were recorded and will be used to inform future management of rare plant species.

54 ENVIRONMENTAL SCIENCES↗

Hanford Site Rare Plant Monitoring Report for Calendar Year 2015

In 2015, two separate rare plant monitoring efforts occurred on the portion of the Hanford Site managed by the U.S. Department of Energy (DOE) (referred to herein as Central Hanford). 1. Upland plants of conservation concern were inventoried concurrently with efforts to map plant communities on Central Hanford. Plants were selected based on 2015 status with the Washington State Department of Natural Resources Natural Heritage Program (WNHP) listings, which are summarized in the bullets below. This effort was led by Mission Support Alliance (MSA) and subcontracted personnel from SEE Botanical Consulting.

54 ENVIRONMENTAL SCIENCES↗

​​Updates to Anaerobic Digestion Pathways for Animal Manure in R&D GREET 2025​

Livestock and poultry manure management in the U.S. is a greenhouse gas (GHG) intensive process, emitting 81.7 MMT CO 2 e in 2022 (1.5% of net U.S. GHG emissions). The primary GHG is methane (CH 4 ), with 2,312 kt released in 2022 (9% of U.S. CH 4 emissions). Manure management methods are commonly categorized by whether they are anaerobic (“wet”) or aerobic (“dry”) techniques. Although dry methods manage the largest share of manure, the majority of GHG emissions are generated during storage of manure in anaerobic conditions – typically in water-filled tanks, pits, or lagoons. There has been a 65% increase in emissions from 1990, primarily due to an increasing cattle population. Also, this rise in population has been coupled with a rise in animal confinement and density, which typically adopt wet manure management methods. Within wet methods, anaerobic bacteria proliferate and decompose volatile solids (VS) within the manure in a process called anaerobic digestion to produce roughly equal mixtures of CH 4 and carbon dioxide (CO 2 ). These GHGs are fugitive, in that they are assumed to be released to the atmosphere and contribute to GHGs within U.S. GHG inventories. If the methane is captured and purified (i.e., “upgraded”) this simultaneously mitigates GHGs that would have otherwise been released and produces a valuable energy product known colloquially as Renewable Natural Gas (RNG). Such processes are acknowledged by U.S. policy through programs such as the U.S. Renewable Fuels Standard (RFS), the federal Clean Fuel Production Credit (45Z), and state clean fuel standards (CFS). In the 45Z and CFS schemes, the GHG emissions of the business-as-usual (BAU) manure management system is taken as a baseline, and credits are received based on GHG reductions relative to this baseline. Thus, estimating the GHG emissions of the BAU scenario (also known as the “counterfactual”) is necessary.

09 BIOMASS FUELS↗

Townsend's Ground Squirrel Conservation on the Hanford Site for Calendar Year 2025

The U.S. Department of Energy (DOE), Hanford Field Office (HFO) conducts ecological monitoring on the DOE-managed portion of Hanford Site (hereby referred to as Central Hanford) to collect and track data needed to ensure compliance with an array of environmental laws, regulations, and policies governing HFO activities. Ecological monitoring data provides baseline information about the plants, animals, and habitat under HFO stewardship on the Hanford Site, which is required for accurate ecological impact assessment decision making under the National Environmental Policy Act and the Comprehensive Environmental Response, Compensation, and Liability Act. In addition, ecological monitoring helps ensure that HFO, its contractors, and other entities conducting activities on the Hanford Site are in compliance with DOE/EIS-0222-F, Final Hanford Comprehensive Land Use Plan Environmental Impact Statement. HFO places priority on monitoring plant and animal species or habitats that have specific regulatory protections or requirements; that are rare and/or declining (federal or state listed endangered, threatened, or sensitive species); or are of significant interest to federal, state, or tribal governments or to the public.

54 ENVIRONMENTAL SCIENCES↗

Outcomes of PAX sapiens-Supported Global Wildlife Data Sharing Conferences for Enhanced One Health Security (GWDSC)

Across two consecutive Global Wildlife Data Sharing Conferences supported by PAX sapiens—Year 1 (May 2024) at Pacific Northwest National Laboratory and Year 2 (2025) in Ciudad Real, Spain—the initiative converted wildlife data sharing from aspiration into operational reality, producing measurable impacts in platform development, data mobilization, standards harmonization, and international partnership formation. The conferences addressed a critical gap in global health security: while 75% of emerging infectious diseases affect both humans and animals and over 60% originate in wildlife, wildlife health surveillance has historically lagged behind human and agricultural sectors due to fragmented databases, inconsistent terminology, uneven capacity, and limited cross-border coordination. By convening practitioners, government agencies, international organizations, academic institutions, and NGOs, the GWDSC catalyzed trust-based relationships and practical workflows that enable earlier detection, better risk assessment, and more effective prevention of threats at the wildlife–domestic animal–human–environment interface.

54 ENVIRONMENTAL SCIENCES↗

Hanford Site Mule Deer Monitoring Report for Fiscal Years 2024 and 2026

The U.S. Department of Energy, Hanford Field Office (HFO) conducts ecological monitoring on the Hanford Site to collect and track data needed to ensure compliance with environmental laws, regulations, and policies governing Department of Energy activities. The vision for the HFOmanaged portion of the Hanford Site, hereby referred to as Central Hanford, focuses not only on the cleanup of nuclear facilities and waste sites but on the protection and restoration of the Hanford Site lands. As the HFO moves toward accomplishing this vision, understanding of the ecological resources present and the need for conservation and/or protection of those resources will be critical for making informed decisions for responsible site stewardship. Ecological monitoring data provides baseline information about the plants, animals, and habitats under HFO stewardship at Central Hanford required for decision-making under the National Environmental Policy Act of 1969 (NEPA) and Comprehensive Environmental Response, Compensation, and Liability Act of 1980.

54 ENVIRONMENTAL SCIENCES↗

Coupling Remote Sensing and Sonar Measurements to Estimate Availability of Suitable Backwater Nursery Habitat for Colorado Pikeminnow

Low-velocity channel-margin habitats serve as important nursery habitats for the endangered Colorado pikeminnow (Ptychocheilus lucius) in the middle Green River between Jensen and Ouray, Utah. These habitats, known as backwaters, are associated with emergent sandbars and are shaped and reformed annually by peak flows. Recent knowledge about backwater characteristics and dynamics was summarized in a synthesis report (Grippo et al. 2015) that was based on detailed annual survey data collected from a relatively small sample of backwaters between 2003 to 2014 and reach-wide evaluations of backwater surface area based on manual interpretation of aerial and satellite imagery. Methods that bridge the gap between the detailed surveys from a small number of backwaters and reach-wide assessments of their surface area would enable an improved assessment of the availability of backwater habitats that provide suitable depths for young-of-the-year (YOY) Colorado pikeminnow.

13 HYDRO ENERGY↗

Burrowing Owl Survey Report for Sandia National Laboratories: 2025

The Burrowing Owl Survey Report for Sandia National Laboratories: 2025 provides data and analysis on occupancy surveys that were conducted during the 2025 survey season. The surveys were conducted in partnership with Kirtland Air Force Base’s Natural Resource Program and the United States Fish and Wildlife Service to maintain and expand on the long-term dataset on burrowing owls on base. Ecology Program biologists conducted roadside census surveys at survey areas previously identified by Kirtland Air Force Base along with several additional survey areas deemed suitable for occupancy. Remote sensor camera monitoring was also conducted on three nest burrows. In total, 12 breeding pairs of burrowing owls were confirmed in 2025. Several owls were confirmed to have leg bands, indicating previous captures and recurrence of these specific individuals on base. Occupancy surveys on burrowing owls continue to provide valuable information on ecosystem health and can serve to inform future management direction and decisions.

54 ENVIRONMENTAL SCIENCES↗

Developing a one-step, acid-free microbial process to recover and separate REE (CRADA Final Report)

The project aimed to develop and test microbial strains for increased bioleaching, bioconcentration, and/or bioaccumulation of REEs, and for enhanced separation of REEs from other metals and of individual REEs from one another. The team secured and tested unconventional REE feedstocks to evaluate strains/processes for bioleaching, bioconcentration, and bioaccumulation, including but not limited to wastewater, geothermal brines, red mud, and electronic waste. purified and chemically analyzed REE granules from the microbial platform; determined metabolite and biosynthetic impacts of growth with REEs and the composition of extracellular material(s) produced by platform strains; optimized the growth process with alternative carbon feedstocks to reduce the cost of the fermentation process; and scaled the process from 1 mL to 10 L.

36 MATERIALS SCIENCE↗

Bull Trout and Westslope Cutthroat Trout movement in a dam tailrace

Populations of Bull Trout Salvelinus confluentus and Westslope Cutthroat Trout Oncorhynchus clarkii lewisi in the Pend Oreille Basin have declined, partly due to fragmentation caused by hydropower dams. This study aimed to analyze the movements and behavior of these species downstream of Albeni Falls Dam over two years to inform fishway design. Radiotracking investigations were conducted to monitor 10 adult Bull Trout and 17 adult Westslope Cutthroat Trout from September 2008 to July 2010. Macro and micro detection zones were delineated to study fine- and large-scale movements in the tailrace. Macro zones included the spillway and powerhouse tailrace areas. Micro zones were nested within macro zones to identify regions close to the dam where fishway structures could be built. Both Bull Trout and Westslope Cutthroat Trout exhibited high mobility in the dam tailrace, transitioning between macro detection zones. Seasonal variations influenced their distribution patterns. During the spring freshet migration season, both species were primarily detected at the left powerhouse micro zone. In sedentary periods (fall/winter and summer), fish actively swam throughout the tailrace, displaying search behavior. These behavior observations suggest that potential fishway entrances located near dam concrete would be effective: the area near the left powerhouse was identified as the optimal construction location. This study highlights the feasibility of designing fish passage structures to mitigate population fragmentation and support species recovery.

13 HYDRO ENERGY↗

U.S. Pacific Coast Workshop Report on Preconstruction Research Recommendations (U.S. Offshore Wind Synthesis of Environmental Effects Research (SEER) Project)

In May 2022, the U.S. Offshore Wind Synthesis of Environmental Effects Research (SEER) project team hosted a stakeholder workshop focused on preconstruction (baseline) research needs for potential floating offshore wind (OSW) energy development on the U.S. Pacific Coast, including California, Oregon, and Washington. Prior to the workshop, the SEER team developed a set of initial synthesized research recommendations that were identified based on a review of relevant, publicly available resources and with advisory group input. The workshop covered three marine life breakout groups on subsequent days to discuss research recommendations related to 1) marine mammals and sea turtles, 2) fish and invertebrates, and 3) birds and bats. As part of the workshop, over a hundred participants from the public and private sectors provided feedback on various aspects of the initial research recommendations, including associated data and knowledge gaps, benefits/limitations of available methods and technologies, and technological advancements or infrastructure needed to address the recommendation. Approximately 1,000 total comments were received on the workshop MURAL boards and were synthesized in this report. Based on workshop feedback, SEER developed a final database of over 500 specific research recommendations based on more than 40 resources. In Fall 2022, the full database and a tool with updated synthesized research recommendations were disseminated on Tethys (https://tethys.pnnl.gov) to assist with informing future funding opportunities and research programming. There is a continued need to improve awareness of the potential environmental effects, monitoring technologies, and management strategies for floating OSW energy development on the U.S. Pacific Coast. Coordination of these activities will require the sustained involvement of multiple stakeholders from across sectors. Beyond the baseline considerations discussed in this workshop, future state-of-the-science activities should be planned to consider research needs across wind energy life cycle phases for all relevant wildlife taxa and associated habitat and ecosystem processes.

17 WIND ENERGY↗

East Tennessee Technology Park 2006 Illness and Injury Surveillance Report

The U.S. Department of Energy’s (DOE) commitment to assuring the health and safety of its workers includes the conduct of illness and injury surveillance activities that provide an early warning system to detect health problems among workers. The Illness and Injury Surveillance Program monitors illnesses and health conditions that result in an absence, occupational injuries and illnesses, and disabilities and deaths among current workers.

54 ENVIRONMENTAL SCIENCES↗

Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomography

Primary cilia mediate sensory signaling in multiple organisms and cell types but have structures adapted for specific roles. Structural defects in them lead to devastating diseases known as ciliopathies in humans. Key to their functions are structures at their base: the basal body, the transition zone, the “Y-shaped links,” and the “ciliary necklace.” We have used cryo-electron tomography with subtomogram averaging and conventional transmission electron microscopy to elucidate the structures associated with the basal region of the “connecting cilia” of rod outer segments in mouse retina. The longitudinal variations in microtubule (MT) structures and the lumenal scaffold complexes connecting them have been determined, as well as membrane-associated transition zone structures: Y-shaped links connecting MT to the membrane, and ciliary beads connected to them that protrude from the cell surface and form a necklace-like structure. These results represent a clearer structural scaffold onto which molecules identified by genetics, proteomics, and superresolution fluorescence can be placed in our emerging model of photoreceptor sensory cilia.

59 BASIC BIOLOGICAL SCIENCES↗