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At least 1,459 records · Page 81
All sourcesNASA NTRSDOE OSTI
DOE OSTI2025

Metagenome-assembled genomes from East River floodplain sediments near Crested Butte, CO, USA (June to September 2017)

Microorganisms play a key role in cycling nutrients and contaminants in the terrestrial environment depending on their genetic potential. Here, we present metagenome-assembled genomes (MAGs) for the bacterial and archaeal community in floodplain sediment samples taken in 2017 in June (flooded conditions) and September (drained conditions) at two locations (MCB1 and MCB3) in an active meander (Meander C) of the East River. Sediment cores were collected from 2 depths, a near-surface, generally unsaturated depth (15-40 centimeter (cm) depth below surface) and a deeper depth influenced by flooding with redoximorphic features (50-88 cm depth below surface). Sediments were homogenized from the ~10 cm cores for microbial analyses. A total of 24 metagenomes were sequenced through the Joint genome institute (JGI) corresponding to 8 samples sequenced in triplicate. These metagenomes can be found under Genomes Online Database (GOLD) sequencing project: Gs0151851. Metagenomes were assembled, binned, and refined using metawrap to generate MAGs (>50% complete and < 10% contamination based on checkM scores). This dataset includes a zip file of 405 MAG fasta files and a csv file with quality, taxonomic classification (Genome Taxonomy Database Release RS220), and metagenome accessions for MAGs. This dataset also includes a file-level metadata (flmd.csv) file that lists each file contained in the dataset with associated metadata and a data dictionary (dd.csv) file that contains column/row headers used throughout the files along with a definition, units, and data type.This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231. Part of this work was performed at SLAC Accelerator Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-76SF00515.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2025

Metagenome-assembled genomes from East River floodplain sediments near Crested Butte, CO, USA (May to September 2018)

Microorganisms play a key role in cycling nutrients and contaminants in the terrestrial environment depending on their genetic potential. Here, we present metagenome-assembled genomes (MAGs) for the bacterial and archaeal community in floodplain sediment samples taken in 2018 in May (flooded conditions) and September (drained conditions) at two locations (MCB1 and MCB3) near the Meander C/Pumphouse floodplain sites of the East River. Sediment cores were collected from 2 depths, a near-surface, generally unsaturated depth (30-40 centimeter (cm) depth below surface) and a deeper depth influenced by flooding with redoximorphic features (70-80 cm depth below surface). Sediments were homogenized from the 10 cm core for microbial analyses. A total of 24 metagenomes were sequenced through the Joint genome institute (JGI) corresponding to 8 samples sequenced in triplicate. These metagenomes can be found under Genomes Online Database (GOLD) sequencing project: Gs0141020. Metagenomes were assembled, binned, and refined using metawrap to generate MAGs (>50% complete and < 10% contamination based on checkM scores). This dataset includes a zip file of 478 MAG fasta files and a csv file with quality, taxonomic classification (Genome Taxonomy Database Release RS220), and metagenome accessions for MAGs. This dataset also includes a file-level metadata (flmd.csv) file that lists each file contained in the dataset with associated metadata and a data dictionary (dd.csv) file that contains column/row headers used throughout the files along with a definition, units, and data type.This work was supported by the Watershed Function Science Focus Area at Lawrence Berkeley National Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-05CH11231. Part of this work was performed at SLAC Accelerator Laboratory funded by the US Department of Energy, Office of Science, Biological and Environmental Research under Contract No. DE-AC02-76SF00515.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2018

Rail-Cask Tests: Normal-Conditionsof- Transport Tests of Surrogate PWR Fuel Assemblies in an ENSA ENUN 32P Cask.

This report describes tests conducted using a full-size rail cask, the ENSA ENUN 32P, involving handling of the cask and transport of the cask via truck, ships, and rail. The purpose of the tests was to measure strains and accelerations on surrogate pressurized water reactor fuel rods when the fuel assemblies were subjected to Normal Conditions of Transport within the rail cask. In addition, accelerations were measured on the transport platform, the cask cradle, the cask, and the basket within the cask holding the assemblies. These tests were an international collaboration that included Equipos Nucleares S.A., Sandia National Laboratories, Pacific Northwest National Laboratory, Coordinadora Internacional de Cargas S.A., the Transportation Technology Center, Inc., the Korea Radioactive Waste Agency, and the Korea Atomic Energy Research Institute. All test results in this report are PRELIMINARY – complete analyses of test data will be completed and reported in FY18. However, preliminarily: The strains were exceedingly low on the surrogate fuel rods during the rail-cask tests for all the transport and handling modes. The test results provide a compelling technical basis for the safe transport of spent fuel.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗
DOE OSTI2019

Dynamical Self-Assembly: Constrained phase separation and mesoscale dynamics in lipid membranes (Final Report)

This final technical report summarizes scientific and technical achievements derived through the the award, DE-FG02-04ER46173 [2013-2016, 2016-2017, and 2017-2018(NCE)], for the grant proposal titled, “Dynamical Self-Assembly: Constrained phase separation and mesoscale dynamics in lipid membranes.” The effort was organized into three major scientific aims: (1) characterize the mechanism of inter-layer coupling of laterally phase-separated domains in stacked lipid bilayers; (2) characterize curvature-dependent dynamic reorganization in membrane milieu; and (3) characterize real-time effects of mechanochemical perturbations on lateral phase separation membrane bilayers. The cumulative weight of the activities, including experimental research and collaborative modeling, disseminated through the publications listed above and presentations, has addressed two over-arching objectives: (a) to abstract a physical science based understanding of fundamental rules that determine dynamic self-assembly processes in lipid bilayer membranes - a versatile class of biologically inspired interfacial complex fluids and (b) to translate these physical principles into quantitative design rules for the development of new classes of membrane-based complex materials that exhibit complex, co-operative, and adaptive behavior.

36 MATERIALS SCIENCE↗
DOE OSTI2022

Thylakoid Assembly and Folded Protein Transport by the Tat Pathway

Assembly of functional photosystems complete with necessary intrinsic and extrinsic proteins requires the function of at least three protein transport pathways in thylakoid membranes. Our research focuses on one of those pathways, a unique and essential protein transport pathway found in the chloroplasts of plants, bacteria, and some archaebacteria, the Twin arginine translocation (Tat) system. The chloroplast Tat (cpTat) system is thought to be responsible for the proper localization of ~50% of thylakoid lumen proteins, several of which are necessary for proper photosystem assembly, maintenance, and function. For example, two components of the extrinsic oxygen evolving complex, PsbP and PsbQ, are transported to the thylakoid lumen via the cpTat system. PsbP and PsbQ have been shown to be important in photosystem II function and maintenance (Yi et al. 2006; Yi et al. 2007). The current project addresses the issues of what forms the translocation conduit, how the translocon complex is arranged and what role Hcf106 and cpTatC may have in the process of forming the conduit.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2022

A Subcritical Assembly for Training and Education Use at the Oak Ridge National Laboratory [Slides]

Sufficient AGN-201M fuel plate material exists at Y-12 to support the development of a new, inherently safe, subcritical assembly for use in NCS training courses at ORNL. Four experiments with the ORNL subcritical assembly are possible and feasible. Future work includes the final design of the core and reflector and split table component design specifications. The ORNL Material Demonstration Facility (MDF) may be used to 3D print ORSA stand/table components and graphite reflector. Researchers will consider fuel coatings for contamination control, rather than an Al can. A visit to Y-12 will be made to determine the location of AGN fuel plates and remaining fuel transportation considerations. Final facility location will be considered

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2020

Feasibility Study for a Proposed Subcritical Assembly at Oak Ridge National Laboratory [Slides]

To provide additional NCS training bandwidth, a simple feasibility study was performed for a proposed, inherently safe, subcritical assembly at ORNL for the US Department of Energy (DOE)/National Nuclear Security Administration (NNSA) NCSP training and education (T&E) program. The NCSP performs subcritical, delayed critical and prompt supercritical experiments to support the NCSP T&E program. ORNL performed a study to examine the feasibility of a subcritical assembly with existing fuel that meets the ANSI/ANS-8.26 standard. Section 7.4 of the standard requires NCS staff to participate in hands-on experiments meant to “…demonstrate how varying the properties of a fissionable material system can affect neutron multiplication.” This training is performed to ensure NCS, and operations staff are aware of the risks involved with conducting operations with fissionable materials outside reactors.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗
DOE OSTI2017

Conservation of Species- and Trait-Based Modeling Network Interactions in Extremely Acidic Microbial Community Assembly

Understanding microbial interactions is essential to decipher the mechanisms of community assembly and their effects on ecosystem functioning, however, the conservation of species- and trait-based network interactions along environmental gradient remains largely unknown. Here, by using the network-based analyses with three paralleled data sets derived from 16S rRNA gene pyrosequencing, functional microarray, and predicted metagenome, we test our hypothesis that the network interactions of traits are more conserved than those of taxonomic measures, with significantly lower variation of network characteristics along the environmental gradient in acid mine drainage. The results showed that although the overall network characteristics remained similar, the structural variation was significantly lower at trait levels. The higher conserved individual node topological properties at trait level rather than at species level indicated that the responses of diverse traits remained relatively consistent even though different species played key roles under different environmental conditions. Additionally, the randomization tests revealed that it could not reject the null hypothesis that species-based correlations were random, while the tests suggested that correlation patterns of traits were non-random. Furthermore, relationships between trait-based network characteristics and environmental properties implied that trait-based networks might be more useful in reflecting the variation of ecosystem function. Taken together, our results suggest that deterministic trait-based community assembly results in greater conservation of network interaction, which may ensure ecosystem function across environmental regimes, emphasizing the potential importance of measuring the complexity and conservation of network interaction in evaluating the ecosystem stability and functioning.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2023

De Novo Assembly and Annotation of 11 Diverse Shrub Willow (Salix) Genomes Reveals Novel Gene Organization in Sex-Linked Regions

Poplar and willow species in the Salicaceae are dioecious, yet have been shown to use different sex determination systems located on different chromosomes. Willows in the subgenus Vetrix are interesting for comparative studies of sex determination systems, yet genomic resources for these species are still quite limited. Only a few annotated reference genome assemblies are available, despite many species in use in breeding programs. Here we present de novo assemblies and annotations of 11 shrub willow genomes from six species. Copy number variation of candidate sex determination genes within each genome was characterized and revealed remarkable differences in putative master regulator gene duplication and deletion. We also analyzed copy number and expression of candidate genes involved in floral secondary metabolism, and identified substantial variation across genotypes, which can be used for parental selection in breeding programs. Lastly, we report on a genotype that produces only female descendants and identified gene presence/absence variation in the mitochondrial genome that may be responsible for this unusual inheritance.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1995

(abstract) Designing for Small Volume Assembly of Advanced Electronics Packages

There is a need for high reliability surface mount assembly processes for aerospace, military, and medical applications. This approach for improving design for producibility and reliability of electronic system assembly processes can be utilized as a starting framework. Described herein is a general methodology to Design for Producibility and Reliability (DFPAR) for very small volume production runs.

assembly processes↗
NASA NTRS2016

Design and Analysis of an X-Ray Mirror Assembly Using the Meta-Shell Approach

Lightweight and high resolution optics are needed for future space-based x-ray telescopes to achieve advances in high-energy astrophysics. Past missions such as Chandra and XMM-Newton have achieved excellent angular resolution using a full shell mirror approach. Other missions such as Suzaku and NuSTAR have achieved lightweight mirrors using a segmented approach. This paper describes a new approach, called meta-shells, which combines the fabrication advantages of segmented optics with the alignment advantages of full shell optics. Meta-shells are built by layering overlapping mirror segments onto a central structural shell. The resulting optic has the stiffness and rotational symmetry of a full shell, but with an order of magnitude greater collecting area. Several meta-shells so constructed can be integrated into a large x-ray mirror assembly by proven methods used for Chandra and XMM-Newton. The mirror segments are mounted to the meta-shell using a novel four point semi-kinematic mount. The four point mount deterministically locates the segment in its most performance sensitive degrees of freedom. Extensive analysis has been performed to demonstrate the feasibility of the four point mount and meta-shell approach. A mathematical model of a meta-shell constructed with mirror segments bonded at four points and subject to launch loads has been developed to determine the optimal design parameters, namely bond size, mirror segment span, and number of layers per meta-shell. The parameters of an example 1.3 m diameter mirror assembly are given including the predicted effective area. To verify the mathematical model and support opto-mechanical analysis, a detailed finite element model of a meta-shell was created. Finite element analysis predicts low gravity distortion and low sensitivity to thermal gradients.

module↗
NASA NTRS2016

The Design, Implementation, and Performance of the Astro-H SXS Aperture Assembly and Blocking Filters

The calorimeter array of the JAXA Astro-H (renamed Hitomi) Soft X-ray Spectrometer (SXS) was designed to provide unprecedented spectral resolution of spatially extended cosmic x-ray sources and of all cosmic x-ray sources in the Fe-K band around 6 keV, enabling essential plasma diagnostics. The properties that make the SXS array a powerful x-ray spectrometer also make it sensitive to photons from the entire electromagnetic band, and particles as well. If characterized as a bolometer, it would have a noise equivalent power (NEP) of less than 4x10(exp -18) W/(Hz)0.5(exp 0.5). Thus it was imperative to shield the detector from thermal radiation from the instrument and optical and UV photons from the sky. Additionally, it was necessary to shield the coldest stages of the instrument from the thermal radiation emanating from the warmer stages. Both of these needs are addressed by a series of five thin-film radiation-blocking filters, anchored to the nested temperature stages, that block long-wavelength radiation while minimizing x-ray attenuation. The aperture assembly is a system of barriers, baffles, filter carriers, and filter mounts that supports the filters and inhibits their potential contamination. The three outer filters also have been equipped with thermometers and heaters for decontamination. We present the requirements, design, implementation, and performance of the SXS aperture assembly and blocking filters.

radiation - blocking filters↗
NASA NTRSDate not supplied

Tessellation and Numerical Simulation of the in-Space Assembled Telescope (iSAT) Reflector

A novel tessellation method has been developed to allow the use of uniform truss modules to form curved or doubly curved surfaces. To illustrate the method, the structural support for the doubly curved in-Space Assembled Telescope primary reflector is developed. The method intentionally leaves gaps at the nodes between identical connecting modules; the gaps are relatively small, on the order of 0.5% of the lattice characteristic cell size. Uniquely shaped nodal connectors called multi-nuts are generated to fill in the nodal gaps and connect adjacent modules. With optimization techniques, the size of these nodal gaps can be minimized to thereby minimize the incurred structural efficiency losses. Finite element analyses of the primary reflector were performed, and the structural efficiency losses amounted to a natural frequency reduction of 5%. The benefits of having uniform truss modules outweigh the structural penalties as it will greatly simplify assembly and lower manufacturing costs.

Tessellation↗
NASA NTRSDate not supplied

Tessellation and Numerical Simulation of the in-Space Assembled Telescope (iSAT) Reflector

A novel tessellation method has been developed to allow the use of uniform truss modules to form curved or doubly curved surfaces. To illustrate the method, the structural support for the doubly curved in-Space Assembled Telescope primary reflector is developed. The method intentionally leaves gaps at the nodes between identical connecting modules; the gaps are relatively small, on the order of 0.5% of the lattice characteristic cell size. Uniquely shaped nodal connectors called multi-nuts are generated to fill in the nodal gaps and connect adjacent modules. With optimization techniques, the size of these nodal gaps can be minimized to thereby minimize the incurred structural efficiency losses. Finite element analyses of the primary reflector were performed, and the structural efficiency losses amounted to a natural frequency reduction of 5%. The benefits of having uniform truss modules outweigh the structural penalties as it will greatly simplify assembly and lower manufacturing costs.

Tessellation↗
NASA NTRSDate not supplied

SOLL-E: A Module Transport and Placement Robot for Autonomous Assembly of Discrete Lattice Structures

This paper presents the design and development of a transport and placement robot that demonstrates autonomous assembly of structural building blocks. The robots are intended to serve as a critical component of automated structural assembly and maintenance systems. The Scaling Omni-directional Lattice Locomoting Explorer (SOLL-E) uses a 5-DoF bipedal inchworm locomotion architecture with locking foot and cargo grippers. The locomotion system employs large magnet gap diameter BLDC motors with moderate timing belt gearing for primary joints, and DC planetary gearmotors for turning. Foot and cargo grippers are identical, with servo-actuated locking mechanisms. Three modular controller boards are used to control these actuators in real-time, with command and telemetry data transferred between the server and each controller board via WiFi. Functionality and performance were evaluated in a ground demonstration.

robotics↗