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At least 1,441 records · Page 80

Results and Implications of Mineralogical Models for Chemical Sediments at Meridiani Planum

The Mars Exploration Rover (MER) "Opportunity" has explored chemically-enriched sedimentary outcrops at Meridiani Planum, Mars. In its first year, three different crater sites - Eagle, Fram and Endurance - have been explored. Nineteen high-interest outcrop rocks were investigated by first grinding a hole to reach the interior (using the Rock Abrasion Tool, RAT), and then conducting APXS (alpha particle x-ray spectrometry) analysis, MB (M ssbauer) analysis, and close up imaging (MI, microscopic imager). Sixteen elements and four Fe-bearing minerals were assayed to good accuracy in each sample, producing 380 compositional data points. The Miniature Thermal Emission Spectrometer (Mini- TES) obtained spectra on outcrop materials which provide direct indication of several mineral classes. Preliminary reports on Eagle crater and three RAT samples have been published. Chemical trends and a derived mineralogical model for all RAT d outcrop samples to date has been developed.

Clark, B. C.↗

Prenatal Centrifugation: A Mode1 for Fetal Programming of Body Weight?

'Fetal programming' is a newly emerging field that is revealing astounding insights into the prenatal origins of adult disease, including metabolic, endocrine, and cardiovascular pathophysiology. In the present study, we tested the hypothesis that rat pups conceived, gestated and born at 2-g have significantly reduced birth weights and increased adult body weights as compared to 1-g controls. Offspring were produced by mating young adult male and female rats that were adapted to 2-g centrifugation. Female rats underwent conception, pregnancy and birth at 2-g. Newborn pups in the 2-g condition were removed from the centrifuge and fostered to non-manipulated, newly parturient dams maintained at 1 -g. Comparisons were made with 1-g stationary controls, also crossfostered at birth. As compared to 1-g controls, birth weights of pups gestated and born at 2-g were significantly reduced. Pup body weights were significantly reduced until Postnatal day (P) 12. Beginning on P63, body weights of 2-g-gestated offspring exceeded those of 1-g controls by 7-10%. Thus, prenatal rearing at 2-g restricts neonatal growth and increases adult body weight. Collectively, these data support the hypothesis that 2-g centrifugation alters the intrauterine milieu, thereby inducing persistent changes in adult phenotype.

Baer, Lisa A.↗

Human Bone-Forming Chondrocytes Cultured in the Hydrodynamic Focusing Bioreactor Retain Matrix Proteins: Similarities to Spaceflight Results

Fracture healing, crucial to a successful Mars mission, involves formation of a cartilaginous fracture callus which differentiates, mineralizes, ossifies and remodels via the endochondral process. Studies of spaceflown and tailsuspended rats found that, without loading, fracture callus formation and cartilage differentiation within the callus were minimal. We found delayed differentiation of chondrocytes within the rat growth plate on Cosmos 1887, 2044, and Spacelab 3. In the current study, differentiation of human bone-forming chondrocytes cultured in the hydrodynamic focusing bioreactor (HFB) was assessed. Human costochondral chondrocytes in suspension were aggregated overnight, then cultured in the HFB for 25 days. Collagen Type II, aggrecan and unsulfated chondroitin were found extracellularly and chondroitin sulfates 4 and 6 within the cell. Lack of secretion was also found in pancreatic cells of spaceflown rats, and in our SL3 studies. The HFB can be used to study cartilage differentiation in simulated microgravity.

Duke, P. J.↗

Device Acquires and Retains Rock or Ice Samples

The Rock Baller is a sample acquisition tool that improves sample retention. The basic elements of the Rock Baller are the tool rotation axis, the hub, the two jaws, and the cutting blades, which are located on each of the jaws. The entire device rotates about the tool rotation axis, which is aligned parallel to the nominal normal direction of the parent rock surface. Both jaws also rotate about the jaw axis, which is perpendicular to the tool rotation axis, at a rate much slower than the rotation about the tool rotation axis. This movement gradually closes the jaws into a nearly continuous hemispherical shell that encloses the sample as it is cut from the parent rock. When required the jaws are opened to release the sample. The hemispherical cutting method eliminates the sample retention problems associated with existing sample acquisition methods that employ conventional cylindrical cutting. The resulting samples are hemispherical, or nearly hemispherical, and as a result the aspect ratio (sample depth relative to sample radius) is essentially fixed. This fixed sample aspect ratio may be considered a drawback of the Rock Baller method, as samples with a higher aspect ratio (more depth, less width) may be considered more scientifically valuable because such samples would allow for a broader inspection of the geological record. This aspect ratio issue can be ameliorated if the Rock Baller is paired with a device similar to the Rock Abrasion Tool (RAT) used on the Mars Exploration Rovers. The RAT could be used to first grind into the surface of the parent rock, after which the Rock Baller would extract a sample from a depth inside the rock that would not have been possible without first using the RAT. Other potential applications for this technology include medical applications such as the removal of tissue samples or tumors from the body, particularly during endoscopic, laparoscopic, or thoracoscopic surgeries.

Giersch, Louis R.↗

Fluorogenic Cell-Based Biosensors for Monitoring Microbes

Fluorogenic cell-based sensor systems for detecting microbes (especially pathogenic ones) and some toxins and allergens are undergoing development. These systems harness the natural signaltransduction and amplification cascades that occur in mast cells upon activation with antigens. These systems include (1) fluidic biochips for automated containment of samples, reagents, and wastes and (2) sensitive, compact fluorometers for monitoring the fluorescent responses of mast cells engineered to contain fluorescent dyes. It should be possible to observe responses within minutes of adding immune complexes. The systems have been shown to work when utilizing either immunoglobulin E (IgE) antibodies or traditionally generated rat antibodies - a promising result in that it indicates that the systems could be developed to detect many target microbes. Chimeric IgE antibodies and rat immunoglobulin G (IgG) antibodies could be genetically engineered for recognizing biological and chemical warfare agents and airborne and food-borne allergens. Genetic engineering efforts thus far have yielded (1) CD14 chimeric antibodies that recognize both Grampositive and Gram-negative bacteria and bind to the surfaces of mast cells, eliciting a degranulation response and (2) rat IgG2a antibodies that act similarly in response to low levels of canine parvovirus.

Curtis, Theresa↗

Permissible Exposure Level for Lunar Dusts: Gaps are Closing

Space faring nations plan to return human explorers to the moon within the next decade. Experience during the Apollo flights suggests that lunar dust will invariably get into the habitat where the finest portion (less than 5 micrometers) could be inhaled by the crew before it is cleared from the atmosphere. NASA is developing a database from which a 6-month, episodic exposure standard for lunar dust can be set. Three kinds of moon dust were prepared from a parent sample of Apollo 14 regolith #14003,96. Our goal was to prepare each type of dust sample with a mean diameter less than 2 m, which is suitable for instillation into the lungs of rats. The three samples were prepared as follows: separation from the parent sample using a fluidized bed, grinding using a jet mill grinder, or grinding with a ball-mill grinder. Grinding simulated restoration of surface activation of dust expected to occur at the surface of the moon on native lunar dust. We used two grinding methods because they seemed to produce different modes of activation. The effects of grinding were preserved by maintaining the dust in ultra-pure nitrogen until immediately before it was placed in suspension for administration to rats. The dust was suspended in physiological saline with 10% Survanta, a lung surfactant. Rats were given intratrachael instillations of the dust suspension at three doses. In addition to the three moon dusts (A, C and E), we instilled the same amount of a negative control (TiO2, B) and a highly-toxic, positive control (quartz, D). These additional mineral dusts were selected because they have well-established and very different permissible exposure levels (PELs). Our goal was to determine where lunar dusts fit between these extremes, and then estimate a PEL for each lunar dust. We evaluated many indices of toxicity to the lung. The figure shows the changes in lactate dehydrogenase (LDH), a marker of cell death, for the five dusts. Benchmark dose software (Version 2.1.2) from the Environmental Protection Agency was used to estimate the 10% effect levels (BMD(sub 10)) using five models. The best-fitting model was used to estimate the optimal BMD(sub 10) (table)

James, John T.↗

Modeling Respiratory Toxicity of Authentic Lunar Dust

The lunar expeditions of the Apollo operations from the 60 s and early 70 s have generated awareness about lunar dust exposures and their implication towards future lunar explorations. Critical analyses on the reports from the Apollo crew members suggest that lunar dust is a mild respiratory and ocular irritant. Currently, NASA s space toxicology group is functioning with the Lunar Airborne Dust Toxicity Assessment Group (LADTAG) and the National Institute for Occupational Safety and Health (NIOSH) to investigate and examine toxic effects to the respiratory system of rats in order to establish permissible exposure levels (PELs) for human exposure to lunar dust. In collaboration with the space toxicology group, LADTAG and NIOSH the goal of the present research is to analyze dose-response curves from rat exposures seven and twenty-eight days after intrapharyngeal instillations, and model the response using BenchMark Dose Software (BMDS) from the Environmental Protection Agency (EPA). Via this analysis, the relative toxicities of three types of Apollo 14 lunar dust samples and two control dust samples, titanium dioxide (TiO2) and quartz will be determined. This will be executed for several toxicity endpoints such as cell counts and biochemical markers in bronchoaveolar lavage fluid (BALF) harvested from the rats.

Santana, Patricia A.↗

The Habitat Demonstration Unit Project: A Modular Instrumentation System for a Deep Space Habitat

NASA is focused on developing human exploration capabilities in low Earth orbit (LEO), expanding to near Earth asteroids (NEA), and finally to Mars. Habitation is a crucial aspect of human exploration, and a current focus of NASA activities. The Habitation Demonstration Unit (HDU) is a project focused on developing an autonomous habitation system that enables human exploration of space by providing engineers and scientists with a test bed to develop, integrate, test, and evaluate habitation systems. A critical feature of the HDU is the instrumentation system, which monitors key subsystems within the habitat. The following paper will discuss the HDU instrumentation system performance and lessons learned during the 2010 Desert Research and Technology Studies (D-RaTS). In addition, this paper will discuss the evolution of the instrumentation system to support the 2011 Deep Space Habitat configuration, the challenges, and the lessons learned of implementing this configuration. In 2010, the HDU was implemented as a pressurized excursion module (PEM) and was tested at NASA s D-RaTS in Arizona [1]. For this initial configuration, the instrumentation system design used features that were successful in previous habitat instrumentation projects, while also considering challenges, and implementing lessons learned [2]. The main feature of the PEM instrumentation system was the use of a standards-based wireless sensor node (WSN), implementing an IEEE 802.15.4 protocol. Many of the instruments were connected to several WSNs, which wirelessly transmitted data to the command and data handling system via a mesh network. The PEM instrumentation system monitored the HDU during field tests at D-RaTS, and the WSN data was later analyzed to understand the performance of this system. In addition, several lessons learned were gained from the field test experience, which fed into the instrumentation design of the next generation of the HDU.

Rojdev, Kristina↗

Desert Research and Technology Studies Exposure of Lotus Coated Electrodynamic Shield Samples

The passive Lotus dust mitigation coating currently being developed at NASA's Goddard Space Flight Center (GSFC), was selected by the Habitation Demonstration Unit Deep Space Habitat (HDU-DSH) for participation in the 2011 Desert Research and Technology Studies (D-RaTS). Based on the unique surface architecture of the Lotus leaf, the nano-engineered Lotus coating seeks to replicate these structures on space flight and habitation surfaces. By decreasing both the surface energy and area for particle attachment, the Lotus coating greatly diminishes dust accumulation on surfaces. This is a problem that can be encountered on lunar, Martian, and asteroid missions. Two different application methods of this coating were tested in summer 2011 at the D-RaTS site: the wet chemistry applied version and combustion chemical vapor deposition (CCVD) applied version. These Lotus coatings, along with two common thermal control coatings, were combined with the active dust mitigation electrodynamic shield (EDS) technology developed at Kennedy Space Center (KSC). The EDS technology uses an electrified grid to remove dust particles from the surface of a Kapton (Trademark) substrate. The Lotus coating and thermal control coatings were applied to these Kapton (Trademark) substrates for testing. The combination of these two innovations was theorized to be an applicable countermeasure for addressing dust accumulation during long-duration human space exploration. This theory was tested and characterized prior to, during, and after D-RaTS exposure.

Margiotta, Danielle V.↗

Desert Research and Technology Studies (DRATS) 2010 Science Operations: Operational Approaches and Lessons Learned for Managing Science during Human Planetary Surface Missions

Desert Research and Technology Studies (Desert RATS) is a multi-year series of hardware and operations tests carried out annually in the high desert of Arizona on the San Francisco Volcanic Field. These activities are designed to exercise planetary surface hardware and operations in conditions where long-distance, multi-day roving is achievable, and they allow NASA to evaluate different mission concepts and approaches in an environment less costly and more forgiving than space.The results from the RATS tests allows election of potential operational approaches to planetary surface exploration prior to making commitments to specific flight and mission hardware development. In previous RATS operations, the Science Support Room has operated largely in an advisory role, an approach that was driven by the need to provide a loose science mission framework that would underpin the engineering tests. However, the extensive nature of the traverse operations for 2010 expanded the role of the science operations and tested specific operational approaches. Science mission operations approaches from the Apollo and Mars-Phoenix missions were merged to become the baseline for this test. Six days of traverse operations were conducted during each week of the 2-week test, with three traverse days each week conducted with voice and data communications continuously available, and three traverse days conducted with only two 1-hour communications periods per day. Within this framework, the team evaluated integrated science operations management using real-time, tactical science operations to oversee daily crew activities, and strategic level evaluations of science data and daily traverse results during a post-traverse planning shift. During continuous communications, both tactical and strategic teams were employed. On days when communications were reduced to only two communications periods per day, only a strategic team was employed. The Science Operations Team found that, if communications are good and down-linking of science data is ensured, high quality science returns is possible regardless of communications. What is absent from reduced communications is the scientific interaction between the crew on the planet and the scientists on the ground. These scientific interactions were a critical part of the science process and significantly improved mission science return over reduced communications conditions. The test also showed that the quality of science return is not measurable by simple numerical quantities but is, in fact, based on strongly non-quantifiable factors, such as the interactions between the crew and the Science Operations Teams. Although the metric evaluation data suggested some trends, there was not sufficient granularity in the data or specificity in the metrics to allow those trends to be understood on numerical data alone.

Eppler, Dean↗

The Antarctic Search for Meteorites: A Model for Deep Space Exploration

In an era of shrinking buying power and reduced flight opportunities, NASA must extract the greatest possible value from all sources of insight into the future of human space exploration. Antarctica is one such source. The history of Antarctic exploration has many political and technical parallels with the development of space, and Antarctica's remoteness and harsh climate make it an excellent proxy for space (e.g., [1,2]). Links between exploration of space and of the Antarctic date back to the International Geophysical Year of 1957-1958, which saw both the launch of Sputnik 1 and the establishment of a station at the South Pole. The Antarctic Search for Meteorites (ANSMET) is an annual expedition to the south polar plateau to collect meteorites. Although its intent is not to simulate a space mission, the handful of astronauts who have participated in ANSMET agree that it is very similar to a long-duration space flight. Independently, NASA and other space agencies have simulated deep space exploration missions in "analog" activities at remote field sites on Earth (e.g., [3]). These include NASA Extreme Environment Mission Operations (NEEMO) [4,5], Desert Research And Technology Studies (Desert RATS) [6,7], and the Pavilion Lake Research Project (PLRP or simply Pavilion Lake) [8]. This report focuses on NEEMO, Desert RATS, and PLRP because of the author's firsthand experience with them. Other noteworthy analogs, such as the arctic Haughton Mars Project and the European Space Agency's underground Cooperative Adventure for Valuing and Exercising human behavior and performance Skills (CAVES), are not treated here. NASA analogs often include fully staffed control centers, astronauts serving as crew, and realistic mission timelines lasting one to two weeks. Analogs have provided key insights into system architectures and operational concepts for the future human exploration of deep space. They have pioneered techniques for human communication with significant speed-of-light delays, for conducting spacewalks on natural objects with negligible surface gravity, and for empowering exploration crews to work with reduced dependence on a ground control center. They have field-tested dozens of emerging technologies including spacewalking tools and full-scale prototype vehicles and habitats. They have provided valuable experience for astronauts preparing for their first space flight, and for flown crewmembers who will take command roles on later flights. Some analogs, especially PLRP, have connected observers in the field with science teams in remotely located control centers to produce high-quality, publishable scientific results. The analogs have accomplished all of this at a tiny fraction of the cost of an actual space flight. This report treats ANSMET as space flight analog. The chapter following this introduction describes ANSMET in depth. The report then presents data on logistics and crew considerations that may be useful for developers of future human space exploration missions. It offers detailed comparisons between ANSMET and past, present, and future space flights on the Space Shuttle, the International Space Station (ISS), and a proposed Mars mission. Those comparisons are intended to complement the work of Eppler [2], who compares ANSMET to the Apollo moon flights. This report also compares ANSMET with the Desert RATS, NEEMO, and PLRP analogs. It then presents observations and makes recommendations related to ANSMET's value as a simulated space mission. The report ends with a short conclusion. The remainder of this introductory chapter provides background material to help readers interpret the rest of the report. It gives brief overviews of Space Shuttle and ISS missions along with information on a notional future human flight to Mars. It also presents the general features of three of NASA's space flight "analog" projects. With those points of reference in place, the chapter concludes with an overview of ANSMET.

Love, Stanley G.↗

Signaling Pathways Involved in Lunar Dust Induced Cytotoxicity

The Moon's surface is covered by a layer of fine, reactive dust. Lunar dust contain about 1-2% of very fine dust (< 3 micron), that is respirable. The habitable area of any lunar landing vehicle and outpost would inevitably be contaminated with lunar dust that could pose a health risk. The purpose of the study is to evaluate the toxicity of Apollo moon dust in rodents to assess the health risk of dust exposures to humans. One of the particular interests in the study is to evaluate dust-induced changes of the expression of fibrosis-related genes, and to identify specific signaling pathways involved in lunar dust-induced toxicity. F344 rats were exposed for 4 weeks (6h/d; 5d/wk) in nose-only inhalation chambers to concentrations of 0 (control air), 2.1, 6.1, 21, and 61 mg/m(exp 3) of lunar dust. Five rats per group were euthanized 1 day, 1 week, 1 month, and 3 months after the last inhalation exposure. The total RNAs were isolated from the blood or lung tissue after being lavaged, using the Qigen RNeasy kit. The Rat Fibrosis RT2 Profile PCR Array was used to profile the expression of 84 genes relevant to fibrosis. The genes with significant expression changes are identified and the gene expression data were further analyzed using IPA pathway analysis tool to determine the signaling pathways with significant changes.

Zhang, Ye↗

Evaluation of Rodent Spaceflight in the NASA Animal Enclosure Module for an Extended Operational Period (up to 35 days)

The National Aeronautics and Space Administration Animal Enclosure Module (AEM) was developed as a self-contained rodent habitat for shuttle flight missions that provides inhabitants with living space, food, water, ventilation, and lighting for shuttle flight missions, and this study reports whether, after minimal hardware modification, the AEM could support an extended term up to 35 days for Sprague-Dawley rats and C57BL/6 female mice for use on the International Space Station. Success was evaluated based on comparison of AEM housed animals to that of vivarium housed and to normal biological ranges through various measures of animal health and well-being, including animal health evaluations, animal growth and body masses, organ masses, rodent food bar consumption, water consumption, and analysis of blood contents. The results of this study confirmed that the AEMs could support 12 adult female C57BL/6 mice for up to 35 days with self-contained RFB and water, and the AEMs could also support 5 adult male Sprague-Dawley rats for 35 days with external replenishment of diet and water. This study has demonstrated the capability and flexibility of the AEM to operate for up to 35 days with minor hardware modification. Therefore, with modifications, it is possible to utilize this hardware on the International Space Station or other operational platforms to extend the space life science research use of mice and rats.

Animal enclosure module↗

Informing Rodent Behavior in Space: Gene Expression and Hyper-Emotionality Following Prenatal Stress

As space exploration pushes our boundaries further away from Earth and for longer durations, we will inevitably require the use of multi-generational studies to continue our expansion. Space is a stressful environment not only due to the deleterious effect of spaceflight on physiology, but also due to confinement, limited social interactions, inherently dangerous circumstances, and many other stresses of an unknown environment. Stress can alter the brain chemistry, and these alterations can affect behavior at all stages of development, but it is especially pronounced during the perinatal period and can have longstanding effects, even into adulthood, which manifest through augmented brain function and psychopathology. This study investigated the nexus of brain chemistry and brain function by observing behavior of adult rats whose mothers were exposed to unpredictable variable prenatal stress (UVPS) while they were in the womb. The UVPS consisted of strobe light, tube restraint, and white noise, and was administered at unpredictable times of the day and also varied in length, both of which were measures taken to prevent habituation to the stressor. The offspring rats were then allowed to reach adulthood and at 90 days were subjected to a series of behavioral tests including novel object, startle response, and an unknown intruder to quantify the adult rats stress response and anxiety. Here we report these results of the behavioral analysis and correlate adult behavioral measures with the expression of genes involved in the hypothalamic-pituitary-adrenal axis, which modulates the animals stress response. We hypothesized that hyper-expression of genes involved in the HPA axis would correlate with the observed anxiety-like behaviors associated with early stress.

Moyer, E. L.↗

Consequences of Prenatal Stress on Appetite Control and the Energy Expenditure Pathway

Established research has illustrated that moderate exposure to stress in the womb influences both adult phonotype and genotype for several physiological pathways, especially in males. Proposed explanations include adaptions made by the fetus resulting from a limited supply of nutrients, referred to as the thrifty phenotype. In this study, we examine this fetal programming effect on the appetite control and energy expenditure pathways in prenatally stressed adult male offspring. Subjects were male rats born from time-mated female rats exposed to unpredictable, variable prenatal stress (UVPS) throughout gestation. An analysis of the adult male rat offspring genetic expression of epididymal fat pads and the plasma concentrations of hormones involved in appetite control and energy expenditure pathways showed a significantly diminished expression of leptin and adiponectin compared to unstressed controls. Leptin and adiponectin are both major hormones involved in the appetite control and energy expenditure pathways, with leptin regulating energy balance due to its function as an inhibitor of hunger, and adiponectin modulating glucose levels and fatty acid breakdown. We observed higher leptin concentrations within the prenatally stressed male plasma, and lower expression of leptin (OB) and adiponectin (ADIPOQ) genes from the epididymal fat pads. We suggest that elevated leptin in the plasma elicited a negative feedback effect on OB expression levels, decreasing their quantification compared to control animals. Further analysis will include plasma quantification of insulin and glucose, as well as expression of ghrelin, a peptide which acts on the central nervous system and the bodys perception of hunger.

appetite control↗

Unpredictable Variable Prenatal Stress Programs Expression of Genes Involved in Appetite Control and Energy Expenditure

Exposure to stress in the womb shapes neurobiological and physiological outcomes of offspring in later life, including body weight regulation and metabolic profiles. Our previous work utilizing a centrifugation-induced hypergravity demonstrated significantly increased (8-15) body mass in male, but not female, rats exposed throughout gestation to chronic 2-g from conception to birth. We reported the same outcome in adult offspring exposed throughout gestation to Unpredictable Variable Prenatal Stress (UVPS). Here we examine gene expression changes using our UVPS model to identify a potential role for prenatal stress in this hypergravity programming effect. Specifically we focused on appetite control and energy expenditure pathways in prenatally stressed adult (90-day-old) male Sprague-Dawley rats. Time-mated female rats were exposed throughout their 22-day pregnancy to UVPS consisting of white noise, strobe light, and tube restraint individually once per day on an unpredictable schedule for 15, 30 or 60 min. To control for potential changes in postnatal maternal care, newborn pups were fostered to non-manipulated, newly parturient dams. At 90-days of age, we analyzed plasma concentrations of hormones involved in appetite control and energy expenditure (leptin and adiponectin), and quantified expression of key genes in epididymal fat pads harvested from adult male offspring and controls. Leptin regulates energy balance by inhibiting hunger, and adiponectin modulates glucose levels and fatty acid breakdown. Our findings indicate significantly elevated plasma leptin concentrations and reduced expression of epididymal fat leptin (OB) and adiponectin (ADIPOQ) genes compared to controls. Analyses presently underway include quantification of plasma insulin and glucose, and the expression of ghrelin, a peptide that acts on the central nervous system and the body's perception of hunger. Collectively, these findings will further understanding of the consequences of UVPS on body weight regulation and metabolism, and provide further insight into the effect of gravity modulation on mammalian fetal development.

Appetite Control↗

Simulated Weightlessness Alters Cardiomyocyte Structure and Transcriptional Regulation of Mediators Related to Immunity and Cardiovascular Disease

Spaceflight and the ensuing fluid shifts, together with an overall reduction in physical activity, lead to acute and latent effects on the cardiovascular system. This current study makes use of the rodent hindlimb unloading (HU) model to determine how factors such as sex, age, and duration of exposure impact cardiac responses to weightlessness. We hypothesize that extended exposure to simulated weightlessness and the ensuing recovery alters cardiac structure and expression of select genes, including those involved in redox signaling which together, negatively impact long-term cardiac tissue health. To begin to test this hypothesis, male and female rats underwent HU at various durations up to 90 days, with a subset reambulated after 90 days of HU. Physiological stress or contractility changes lead to alterations in ventricular cardiomyocyte size and ventricular wall thickness to adapt to greater functional demand and mitigate mechanical stress to ventricular tissue; under certain conditions, these changes also may mark progression to cardiac failure. Hence, left ventricular cardiomyocyte size (cardiomyocyte cross sectional area, CSA) was quantified to determine if HU leads to structural adaptation responses in cardiac tissue and if age and sex had any impact on this outcome. Cardiomyocyte CSA of older males (9 months) were altered by HU in a time-dependent manner, where HU led to decreases in CSA at 14 days and increases at 90 days. In contrast, younger males (3 months) did not show any changes at day 14 of HU. CSA of females (3 months) was increased in response to short-term HU (14 days) suggesting sex-dependence of structural changes. In older HU males, cardiomyocyte CSA was comparable to controls after 90 days of re-ambulation. Levels of the DNA oxidative damage marker, 8-hydroxydeoxyguanosine (8-OHdG) were greater in left ventricular tissue of females that underwent HU compared to sex-matched controls, while there were no such differences in older or younger males. To gain insight into the signals that drive cardiac adaptations to HU, global transcriptomic analysis (RNAseq) was performed on left ventricular tissue of older males that underwent 14 days of HU. Short-term simulated weightlessness led to differential expression of genes involved in immune and pro-inflammatory signaling. A subset of these genes play a role in autoimmune and cardiovascular disease and are targets of current drugs used to treat bradycardia, hypertension, atherosclerosis and rheumatoid arthritis, amongst others. Oxidative damage/redox signaling pathways were not enriched at the timepoint tested in older males. Since young females displayed greater oxidative damage to DNA, activation of oxidative stress responses at earlier or later time points cannot be ruled out. In summary, simulated weightlessness in adult rats caused changes in cardiomyocyte structure in a sex and age-dependent manner, and the transcriptional regulation of key mediators of immunity and cardiovascular disease, meriting further study to define cardiac risks for interplanetary travel of human crew. Our findings also confirm the value of the rat HU model for cardiac health and countermeasure research.

Tahimic, Candice↗

Interstellar Dust Grain Alignment

Dust induced polarization is an efficient probe of the interstellar magnetic field. The combination of short wavelength dichroic extinction polarimetry with far-infrared/sub-mm wave emission observations, using modern instruments, allow us to measure the polarization from diffuse gas to star forming cores. With our improved understanding of the physics of grain alignment we can now better interpret the observations and evaluate the importance of the magnetic field. Radiative Alignment Torque (RAT) grain-alignment has been observationally confirmed, but some aspects and details are still being clarified. The quantitative theoretical frame work provided by RAT theory, also allows long standing questions about the interstellar environment and dust characteristics to be addressed anew - including dust composition and structure. I will review the basics of RAT alignment and its observational support and discuss some of the open questions of dust induced polarization.

Andersson, B.-G.↗