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All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Elucidating Photoinduced Processes of Photosystem I Via Multidimensional Electronic and Vibrational Spectroscopies

This project was motivated by an overarching goal to elucidate the mechanism of energy and electron transfer that governs the efficient charge separation in photosystem I (PSI) complexes. PSI is a natural light harvesting complex that drives oxygenic photosynthesis in plants, algae, and cyanobacteria. It uses ~300 tightly packed chlorophylls (Chls) to absorb photons, transfer the excitation energy to the reaction center (RC), and generate a charge separated state with near unity quantum efficiency (QE). A better understanding of the mechanism of energy transfer and charge separation in PSI is required for understanding the high QE of natural light harvesting complexes, and it could lead to the further development of artificial photosynthetic systems for solar energy conversion and modification of light harvesting complexes to improve crop yields. We applied two-dimensional optical spectroscopies to different cyanobacterial photosystem I complexes, including PSI complexes that contain Chl f molecules, to map energy transfer pathways and gain insight into the efficient light harvesting of PSI. We used two-dimensional electronic spectroscopies (2DES) to map energy transfer in Chl a and Chl f containing PSI complexes. To investigate the Chl f PSI complexes, we modified our spectrometer to probe the lower energy states associated with Chl f molecules. We interpreted the 2DES spectra through global analysis procedures to generate maps of energy transfer. We also constructed a two-dimensional electronic vibrational (2DEV) spectrometer that will be used to investigate charge transfer transitions and dynamics within PSI complexes. Measurements were performed on model systems to establish general data analysis procedures for interpreting 2D spectra and gain insight into protein cofactor interactions.

14 SOLAR ENERGY↗
NASA NTRS2002

Response of thyroid follicular cells to gamma irradiation compared to proton irradiation: II. The role of connexin 32

The objective of this study was to determine whether connexin 32-type gap junctions contribute to the "contact effect" in follicular thyrocytes and whether the response is influenced by radiation quality. Our previous studies demonstrated that early-passage follicular cultures of Fischer rat thyroid cells express functional connexin 32 gap junctions, with later-passage cultures expressing a truncated nonfunctional form of the protein. This model allowed us to assess the role of connexin 32 in radiation responsiveness without relying solely on chemical manipulation of gap junctions. The survival curves generated after gamma irradiation revealed that early-passage follicular cultures had significantly lower values of alpha (0.04 Gy(-1)) than later-passage cultures (0.11 Gy(-1)) (P < 0.0001, n = 12). As an additional way to determine whether connexin 32 was contributing to the difference in survival, cultures were treated with heptanol, resulting in higher alpha values, with early-passage cultures (0.10 Gy(-1)) nearly equivalent to untreated late-passage cultures (0.11 Gy(-1)) (P > 0.1, n = 9). This strongly suggests that the presence of functional connexin 32-type gap junctions was contributing to radiation resistance in gamma-irradiated thyroid follicles. Survival curves from proton-irradiated cultures had alpha values that were not significantly different whether cells expressed functional connexin 32 (0.10 Gy(-1)), did not express connexin 32 (0.09 Gy(-1)), or were down-regulated (early-passage plus heptanol, 0.09 Gy(-1); late-passage plus heptanol, 0.12 Gy(-1)) (P > 0.1, n = 19). Thus, for proton irradiation, the presence of connexin 32-type gap junctional channels did not influence their radiosensitivity. Collectively, the data support the following conclusions. (1) The lower alpha values from the gamma-ray survival curves of the early-passage cultures suggest greater repair efficiency and/or enhanced resistance to radiation-induced damage, coincident with the expression of connexin 32-type gap junctions. (2) The increased sensitivity of FRTL-5 cells to proton irradiation was independent of their ability to communicate through connexin 32 gap junctions. (3) The fact that the beta components of the survival curves from both gamma rays and proton beams were similar (average 0.022 +/- 0.008 Gy(-2), P > 0.1, n = 39) suggests that at higher doses the loss of viability occurs at a relatively constant rate and is independent of radiation quality and the presence of functional gap junctions.

Non-NASA Center↗
NASA NTRS2000

A deficiency in DNA repair and DNA-PKcs expression in the radiosensitive BALB/c mouse

We have studied the efficiency of DNA double strand break (DSB) rejoining in primary cells from mouse strains that show large differences in in vivo radiosensitivity and tumor susceptibility. Cells from radiosensitive, cancer-prone BALB/c mice showed inefficient end joining of gamma ray-induced DSBs as compared with cells from all of the other commonly used strains and F1 hybrids of C57BL/6 and BALB/c mice. The BALB/c repair phenotype was accompanied by a significantly reduced expression level of DNA-PKcs protein as well as a lowered DNA-PK activity level as compared with the other strains. In conjunction with published reports, these data suggest that natural genetic variation in nonhomologous end joining processes may have a significant impact on the in vivo radiation response of mice.

Non-NASA Center↗
NASA NTRS2017

Security Vulnerability Profiles of NASA Mission Software: Empirical Analysis of Security Related Bug Reports

NASA develops, runs, and maintains software systems for which security is of vital importance. Therefore, it is becoming an imperative to develop secure systems and extend the current software assurance capabilities to cover information assurance and cybersecurity concerns of NASA missions. The results presented in this report are based on the information provided in the issue tracking systems of one ground mission and one flight mission. The extracted data were used to create three datasets: Ground mission IVV issues, Flight mission IVV issues, and Flight mission Developers issues. In each dataset, we identified the software bugs that are security related and classified them in specific security classes. This information was then used to create the security vulnerability profiles (i.e., to determine how, why, where, and when the security vulnerabilities were introduced) and explore the existence of common trends. The main findings of our work include:- Code related security issues dominated both the Ground and Flight mission IVV security issues, with 95 and 92, respectively. Therefore, enforcing secure coding practices and verification and validation focused on coding errors would be cost effective ways to improve mission's security. (Flight mission Developers issues dataset did not contain data in the Issue Category.)- In both the Ground and Flight mission IVV issues datasets, the majority of security issues (i.e., 91 and 85, respectively) were introduced in the Implementation phase. In most cases, the phase in which the issues were found was the same as the phase in which they were introduced. The most security related issues of the Flight mission Developers issues dataset were found during Code Implementation, Build Integration, and Build Verification; the data on the phase in which these issues were introduced were not available for this dataset.- The location of security related issues, as the location of software issues in general, followed the Pareto principle. Specifically, for all three datasets, from 86 to 88 the security related issues were located in two to four subsystems.- The severity levels of most security issues were moderate, in all three datasets.- Out of 21 primary security classes, five dominated: Exception Management, Memory Access, Other, Risky Values, and Unused Entities. Together, these classes contributed from around 80 to 90 of all security issues in each dataset. This again proves the Pareto principle of uneven distribution of security issues, in this case across CWE classes, and supports the fact that addressing these dominant security classes provides the most cost efficient way to improve missions' security. The findings presented in this report uncovered the security vulnerability profiles and identified the common trends and dominant classes of security issues, which in turn can be used to select the most efficient secure design and coding best practices compiled by the part of the SARP project team associated with the NASA's Johnson Space Center. In addition, these findings provide valuable input to the NASA IVV initiative aimed at identification of the two 25 CWEs of ground and flight missions.

vulnerability↗
NASA NTRS2003

Advanced Data Acquisition Systems

Current and future requirements of the aerospace sensors and transducers field make it necessary for the design and development of new data acquisition devices and instrumentation systems. New designs are sought to incorporate self-health, self-calibrating, self-repair capabilities, allowing greater measurement reliability and extended calibration cycles. With the addition of power management schemes, state-of-the-art data acquisition systems allow data to be processed and presented to the users with increased efficiency and accuracy. The design architecture presented in this paper displays an innovative approach to data acquisition systems. The design incorporates: electronic health self-check, device/system self-calibration, electronics and function self-repair, failure detection and prediction, and power management (reduced power consumption). These requirements are driven by the aerospace industry need to reduce operations and maintenance costs, to accelerate processing time and to provide reliable hardware with minimum costs. The project's design architecture incorporates some commercially available components identified during the market research investigation like: Field Programmable Gate Arrays (FPGA) Programmable Analog Integrated Circuits (PAC IC) and Field Programmable Analog Arrays (FPAA); Digital Signal Processing (DSP) electronic/system control and investigation of specific characteristics found in technologies like: Electronic Component Mean Time Between Failure (MTBF); and Radiation Hardened Component Availability. There are three main sections discussed in the design architecture presented in this document. They are the following: (a) Analog Signal Module Section, (b) Digital Signal/Control Module Section and (c) Power Management Module Section. These sections are discussed in detail in the following pages. This approach to data acquisition systems has resulted in the assignment of patent rights to Kennedy Space Center under U.S. patent # 6,462,684. Furthermore, NASA KSC commercialization office has issued licensing rights to Circuit Avenue Netrepreneurs, LLC , a minority-owned business founded in 1999 located in Camden, NJ.

Perotti, J.↗
NASA NTRS2019

Large-Scale Boundary Layer Ingesting Propulsor Research

NASA’s Advanced Air Transport Technology (AATT) project is investigating boundary layer ingesting (BLI) propulsors for advanced subsonic commercial vehicle concepts to enable the reduction of fuel burn. A multidisciplinary team of researchers from NASA, United Technologies Research Center (UTRC), Virginia Polytechnic University, and the Air Force Arnold Engineering Development Complex developed and tested an embedded BLI inlet and distortion-tolerant fan (BLI2DTF) system in the NASA Glenn Research Center (GRC) 8- foot by 6-foot (8x6) transonic wind tunnel. The test demonstrated the component performance goals necessary for an overall fuel burn reduction of 3 to 5 percent on a large hybrid wing body (HWB) aircraft. Special test equipment, including a raised floor with flow effectors and a bleed system, was developed for use in the 8x6 to produce the appropriate incoming boundary layer representative of an HWB application. Detailed measurements were made to determine the inlet total pressure loss and distortion, fan stage efficiency, and aeromechanic performance including blade vibration stress and displacement response. Results from this test were used as input to a vehicle-level system study performed by the AATT project to assess the impact of BLI on an alternative advanced concept aircraft referred to as the NASA D8 (ND8), which is somewhat similar to the HWB in its integration of the propulsor. This paper will provide an overview of the project timeline, special test equipment needed in the wind tunnel to develop the appropriate incoming boundary layer, and the difficulties in designing a propulsor for the test. The paper will conclude with some representative aerodynamic and aeromechanic data from the test itself and conclude with how this data was used in the ND8 system study.

Aerodynamic performance↗
NASA NTRS2019

Large-Scale Boundary Layer Ingesting Propulsor Research

_NASA's Advanced Air Transport Technology (AATT) project is investigating boundary layer ingesting (BLI) propulsors for advanced subsonic commercial vehicle concepts to enable the reduction of fuel burn. A multidisciplinary team of researchers from NASA, United Technologies Research Center (UTRC), Virginia Polytechnic University, and the Air Force Arnold Engineering Development Complex developed and tested an embedded BLI inlet and distortion-tolerant fan (BLI2DTF) system in the NASA Glenn Research Center (GRC) 8-foot by 6-foot (8x6) transonic wind tunnel. The test demonstrated the component performance goals necessary for an overall fuel burn reduction of 3 to 5 percent on a large hybrid wing body (HWB) aircraft. Special test equipment, including a raised floor with flow effectors and a bleed system, was developed for use in the 8x6 to produce the appropriate incoming boundary layer representative of an HWB application. Detailed measurements were made to determine the inlet total pressure loss and distortion, fan stage efficiency, and aeromechanic performance including blade vibration stress and displacement response. Results from this test were used as input to a vehicle-level system study performed by the AATT project to assess the impact of BLI on an alternative advanced concept aircraft referred to as the NASA D8 (ND8), which is somewhat similar to the HWB in its integration of the propulsor. This paper will provide an overview of the project timeline, special test equipment needed in the wind tunnel to develop the appropriate incoming boundary layer, and the difficulties in designing a propulsor for the test. The paper will conclude with some representative aerodynamic and aeromechanic data from the test itself and conclude with how this data was used in the ND8 system study.

Celestina, Mark L.↗
DOE OSTI2025

Lost and Found: Rediscovering Microbiome-Associated Phenotypes that Reshape Agricultural Sustainability

Modern agriculture faces an urgent need to improve nutrient use efficiency while reducing environmental impacts. Here, we show that ancestral traits controlling rhizosphere microbiome functions can be reintroduced into elite maize through targeted teosinte introgressions. Using near-isogenic lines, we mapped microbiome-associated phenotypes (MAPs) derived from teosinte that suppress nitrification and denitrification—key microbial processes contributing to nitrogen loss. These introgressions altered root exudate chemistry, resulting in distinct microbial assemblies and enhanced nitrogen retention. We identified candidate loci and metabolites responsible for suppressive activity and demonstrated their functional effects in vitro. Our findings reveal a genetic and biochemical basis for rewilding microbiome-mediated ecosystem services in crops, offering a scalable path toward sustainable nutrient management in global agriculture. ---- These maize root exduate metabolomics data are a subset of this larger project and make up a phenotyping for candidate lines.

Favela, Alonso [School of Plant Sciences, Universi↗
DOE OSTI2021

Topical Report – Findings on Subtask 2.7 – Wet ESP and Aerosol Testing at Coal Creek Station

Growing concerns over the impact of CO 2 emissions from combustion sources on global climate change have prompted numerous research and development projects aimed at developing cost-effective technologies for CO 2 capture. One family of technologies being demonstrated at pilot and full scale globally is postcombustion carbon capture (PCCC) systems that employ amine-based solvents. The captured CO 2 can be compressed and permanently stored underground or used for enhanced oil recovery. The proximity of North Dakota’s lignite-fired fleet of power plants to potential CO 2 storage options creates a unique atmosphere for PCCC within the state. However, the unique components present in lignite flue gas present a challenge for large-scale PCCC at North Dakota power plants by contributing to aerosol formation. Aerosols can negatively impact the long-term performance of amine-based solvents for CO 2 capture. Amine-based solvents are volatile, and flue gas particulate provides nucleation sites where amine vapors can condense as aerosols. Because aerosols cannot be easily captured at the column outlet using conventional technologies, the amine-laden aerosols escape the system and lead to accelerated solvent losses. Moreover, particulate components can chemically react with amines to form degradation products that can permanently deactivate the amine, cause fouling, and lead to hazardous emissions. Many of the elements that have been shown to catalyze solvent degradation are present in lignite coals and can exacerbate solvent replacement economics. Understanding this issue is critical to the implementation of solvent-based CO 2 capture systems as applied to lignite-fired generation systems. The Energy & Environmental Research Center (EERC) designed and carried out this project to fully characterize aerosol behavior with various control technologies installed to better optimize aerosol mitigation technology for CO 2 capture. To meet the goal of this project, the following objectives were identified: Determine the effectiveness of a wet electrostatic precipitator (WESP) on mitigating formation of problematic aerosols at Great River Energy’s Coal Creek Station, upstream of the PCCC system. Determine the effectiveness of the Mitsubishi Heavy Industries (MHI) proprietary amine emission reduction unit (AERU) as a postcapture solvent recovery system for reducing aerosol emissions and extending solvent life downstream of the PCCC system. Determine the impact of aerosols on the efficiency and degradation products of both commercial and advanced solvents within the PCCC system. Work was conducted at Coal Creek Station Unit 1 using a slipstream of flue gas from the outlet of the plant’s flue gas desulfurization (FGD) unit. Flue gas was routed through a pilot-scale FGD unit to remove SO 2 to very low levels (~1 ppm) and then through a direct contact cooler (DCC) to further cool the gas and to remove moisture. The gas exiting the DCC was then optionally routed through a WESP before passing to the CO 2 absorber columns. The MHI solvent was used to scrub CO 2 from the slipstream through a set of two absorber columns. The rich solvent was regenerated in a stripper column by heating to drive off captured CO 2 . Flue gas exiting the absorber column was routed to MHI’s proprietary AERU to recover entrained solvent. The system operated using a catch-and-release method where the CO 2 was separated to provide data on the process, but the captured CO 2 was released back into the host site stack. Particulate was measured, collected, and analyzed from multiple locations throughout the pilot-scale system. Unlike the performance observed in prior work, the inlet FGD and DCC did not remove significant particulate matter from the flue gas. This appears to be due to a difference in the nature of the particulate. The DCC seemed to increase particulate size and count, most likely owing to water condensing onto the surfaces of fly ash particles. When the WESP was operated, it achieved >95% particulate capture. Very little particulate matter or indications of solvent were detected at the AERU outlet. When operating with advanced KS 21 solvent, the particulate material at the AERU outlet was even further decreased. Solvent analysis showed that some species derived from flue gas and ash were slowly concentrating in the solvent over the duration of the test. The levels observed were reported to be within expected ranges and were not of concern to MHI. A high-level techno-economic assessment of installing CO 2 capture at Coal Creek Station suggested that, when using a standard monoethanolamine (MEA)-based solution with simple heat integration, the energy penalty to net generation would be 34%. The bulk of this was due to steam losses for regenerating solvent, followed by parasitic electrical demand for CO 2 compression and then by increased parasitic load for pushing flue gas through the absorber column. These demands could be decreased with a more advanced solvent that exhibits lower heat of regeneration and lower pressure drop than does a simple MEA solution. Further energy could be saved with more thorough heat integration to recover useful energy from the steam used for solvent regeneration. Installing a WESP was predicted to increase the cost of electricity by nearly $5/MWh. This would become cost-effective if solvent losses were roughly 10 times the baseline estimate when not using a WESP but could be returned to baseline by installing the WESP. Piping CO 2 for storage in more favorable geology could help with carbon capture and storage economics. Although storing off-site would necessitate construction of a CO 2 transport pipeline, the cost of this pipeline might be more than offset by reducing the number of wells required, the depths of the wells required, and the electrical demand for the CO 2 compressor. Additional factors that favor off-site storage costs include smaller expected CO 2 plume sizes, which translates to less monitoring and fewer landowner agreements. More detailed assessment of the specific geology in the region under and around Coal Creek Station would be needed to accurately assess the costs and benefits of different storage site options. This subtask was cofunded through the Energy & Environmental Research Center–U.S. Department of Energy Joint Program on Research and Development for Fossil Energy-Related Resources Cooperative Agreement No. DE-FE0024233. Nonfederal funding was provided by the North Dakota Industrial Commission.

Strege, Joshua↗
NASA NTRS2013

Wireless Integrated Microelectronic Vacuum Sensor System

NASA Stennis Space Center's (SSC's) large rocket engine test facility requires the use of liquid propellants, including the use of cryogenic fluids like liquid hydrogen as fuel, and liquid oxygen as an oxidizer (gases which have been liquefied at very low temperatures). These fluids require special handling, storage, and transfer technology. The biggest problem associated with transferring cryogenic liquids is product loss due to heat transfer. Vacuum jacketed piping is specifically designed to maintain high thermal efficiency so that cryogenic liquids can be transferred with minimal heat transfer. A vacuum jacketed pipe is essentially two pipes in one. There is an inner carrier pipe, in which the cryogenic liquid is actually transferred, and an outer jacket pipe that supports and seals the vacuum insulation, forming the "vacuum jacket." The integrity of the vacuum jacketed transmission lines that transfer the cryogenic fluid from delivery barges to the test stand must be maintained prior to and during engine testing. To monitor the vacuum in these vacuum jacketed transmission lines, vacuum gauge readings are used. At SSC, vacuum gauge measurements are done on a manual rotation basis with two technicians, each using a handheld instrument. Manual collection of vacuum data is labor intensive and uses valuable personnel time. Additionally, there are times when personnel cannot collect the data in a timely fashion (i.e., when a leak is detected, measurements must be taken more often). Additionally, distribution of this data to all interested parties can be cumbersome. To simplify the vacuum-gauge data collection process, automate the data collection, and decrease the labor costs associated with acquiring these measurements, an automated system that monitors the existing gauges was developed by Invocon, Inc. For this project, Invocon developed a Wireless Integrated Microelectronic Vacuum Sensor System (WIMVSS) that provides the ability to gather vacuum-gauge measurements automatically and wirelessly, in near-real time - using a low-maintenance, lowpower sensor mesh network. The WIMVSS operates by using a self-configuring mesh network of wireless sensor units. Mesh networking is a type of networking where each sensor or node can capture and disseminate its own data, but also serve as a relay to receive and transmit data from other sensors. Each sensor node can synchronize with adjacent sensors, and propagate data from one sensor to the next, until the destination is reached. In this case, the destination is a Network Interface Unit (NIU). The WIMVSS sensors are mounted on the existing vacuum gauges. Information gathered by the sensors is sent to the NIU. Because of the mesh networking, if a sensor cannot directly send the data to the NIU, it can be propagated through the network of sensors. The NIU requires antenna access to the sensor units, AC power, and an Ethernet connection. The NIU bridges the sensor network to a WIMVSS server via an Ethernet connection. The server is configured with a database, a Web server, and proprietary interface software that makes it possible for the vacuum measurements from vacuum jacketed fluid lines to be saved, retrieved, and then displayed from any Web-enabled PC that has access to the Internet. Authorized users can then simply access the data from any PC with Internet connection. Commands can also be sent directly from the Web interface for control and maintenance of the sensor network. The technology enabled by the WIMVSS decreases labor required for gathering vacuum measurements, increases access to vacuum data by making it available on any computer with access to the Internet, increases the frequency with which data points can be acquired for evaluating the system, and decreases the recurring cost of the sensors by using off-the-shelf components and integrating these with heritage vacuum gauges.

Krug, Eric↗
NASA NTRS2017

One Step Closer to Mars with Aquaponics: Cultivating Citizen Science in K12 Schools

The Microbial Ecology and Biogeochemistry Research Laboratory at NASA Ames Research Center focuses primarily on the nutrient cycling and diversity of complex microbial communities. NASA is interested in the composition and functioning of microbial mat communities as these processes fundamentally shape the form and function of these analogs for the earliest forms of life on Earth (3.6 billion years ago), and likely will on other planets as well. Aquaponics systems are supported by microbial communities who perform many complex ecosystem services, including cycling nitrogen. Microbes are integral to the stability and productivity of aquaponics systems, which are analogous to microbial communities in food production systems that are essential for building efficient life support systems for long-distance space travel. Students at Meadow Park Middle School created 10 parallel aquaponics systems and took temporal microbial samples to characterize whether any macro-ecology variables impacted or changed the microbial diversity of these systems. Students additionally created a website so that other classrooms can pursue similar projects in their own schools (https://go.nasa.gov/2uJhxmF). Our lab at NASA Ames has sequenced water samples from each of the 10 tanks at 3 timepoints using a MinION sequencer. MPMS students will be involved in the analysis of the bioinformatics data generated through this collaboration. Our ongoing collaboration aims to collect and analyze data in the classroom setting that has utility for research scientists, while involving students as collaborators in the research process.

Kolattukudy, Maria↗
NASA NTRS1999

A Diagnostic Analysis of the Kennedy Space Center LDAR Network: Data Characteristics - 1

An analytical framework is developed in which to analyze climatological VHF (66 MHz) radiation measurements taken by the Kennedy Space Center Lightning Detection and Ranging (LDAR) network. A 19-month noise-filtered sample of LDAR observations is examined using this framework. It is found that the climatological VHF source density as observed by LDAR falls off approximately 10 dB every 71 km of ground range away from the network centroid (a 31 km e-folding scale). From this framework it is inferred that the underlying source distribution is likely inverse exponential in amplitude, with a log-slope of 126-147 mW(sup -1/2). The underlying vertical distribution of VHF sources is approximately normally distributed with a mean altitude of 9 km and a standard deviation of 2.7 km; this implies that the loss of below-horizon sources has a negligible effect on column-integrated source densities within 200 km ground range. At medium to far ranges, location errors are primarily radial and have a slightly asymmetric distribution whose first moment increases as range squared. Error moments estimated from observed lightning are significantly higher than those from aircraft-based signal generator or analytic solution estimates, suggesting that timing errors arising from poor signal identification and discrimination may dominate over timing errors arising from nominal sensor resolution. The VHF source properties of individual LDAR-observed flashes are computed and an analytic expression for flash detection efficiency vs. range is derived. This reveals nearly constant flash detection efficiency to 80-94 km range from the network centroid.

Boccippio, D. J.↗
NASA NTRS1998

Steps Towards the Integration of Conflict Resolution with Metering and Scheduling

NASA Ames Research Center is developing decision support tool technology for air traffic controllers to improve the efficiency and capacity of National Airspace System. The goal is to provide technology, tools and procedures that result in the highest possible level of user preferred trajectories whenever possible with safe and efficient traffic management when necessary. The work is being conducted under the NASA Advanced Air Transportation Technology Program in cooperation with the FAA through the Inter-Agency Integrated Product Team. The objective is to develop technology and procedures that lead towards a seamless integration of conflict resolution with metering and scheduling for arrival aircraft and en route aircraft that are under metering restrictions. A requirement is that the integration incorporate user preferred trajectories. The ultimate goal is the implementation and validation of the Descent Advisor (DA) concept which provides clearance advisories to a sector controller that simultaneously meet metering constraints, are conflict free, incorporate a user preferred (e.g., minimum fuel) descent profile, and generally require no further corrective clearance as the aircraft transitions from en route cruise into the TRACON. The DA concept may also be applied to en route aircraft under metering constraints, e.g., miles-in-trail. To achieve the DA concept a stepwise development and field evaluation is anticipated. This paper addresses the initial steps towards implementation of the DA. The Traffic Management Advisor (TMA) computes arrival time sequence and required delay information for display to the sector controller during periods when arrivals must be metered due to landing rate restrictions at the airport. The Initial Conflict Probe (ICP) compares trajectory predictions for all aircraft and alerts the controller when any two aircraft are predicted to violate separation standards (5 mi. and 2000 ft. in en route airspace). ICP also includes a trial planning function allowing the controller to develop and check a separate "what if" trajectory for conflict resolution. TMA and ICP currently operate independent of one another and have separate controller displays. The TMA meter list is on the radar controller's plan view display. The ICP is still under development, but the current concept calls for a list of predicted conflicts at the data controller position. The research described herein address two steps towards the implementation of DA. The first is to develop a concept for integrated display of conflict and metering information on a controller's display. The objective is to provide the controller with situational awareness of one problem while he develops a solution to the other. The next step is to expand the concept for display of automated clearance advisories for one problem (e.g., metering) which take into account the other problems (e.g., conflicts). Information to be communicated between TMA and ICP to facilitate' manual or automated advisories is being identified as the concept matures. In order to study the ICP/TMA integration concept the CTAS conflict probe capability has been adapted to Ft. Worth Center. The system is being validated in the laboratory with all-track data and for non -interference with TMA, TMA is already running as a daily use prototype at Ft. Worth Center. A laboratory prototype system has been developed under the CTAS baseline which combines conflict and metering information on a common user interface. Elements of the user interface are shown in Figure 1. In this simple illustration the user sees the simultaneous effect of a trial plan on meter fix delay and conflict status. Delay information is shown in the aircraft flight data block, the meter list, and the TMA timeline. Experience during Descent Advisor development and observations at Ft. Worth Center high and low attitude arrival sectors during metering suggests that manual trial planning will be unworkable during rush periods due to high controller workload.

McNally, B. David↗
DOE OSTI2025

Data for Discovery, Characterization, and Application of Chromosomal Integration Sites for Stable Heterologous Gene Expression in Rhodotorula toruloides

Rhodotorula toruloides is a non-model, oleaginous yeast uniquely suited to produce acetyl-CoA-derived chemicals. However, the lack of well-characterized genomic integration sites has impeded the metabolic engineering of this organism. Here we report a set of computationally predicted and experimentally validated chromosomal integration sites in R. toruloides . We first implemented an in silico platform by integrating essential gene information and transcriptomic data to identify candidate sites that meet stringent criteria. We then conducted a full experimental characterization of these sites, assessing integration efficiency, gene expression levels, impact on cell growth, and long-term expression stability. Among the identified sites, 12 exhibited integration efficiencies of 50% or higher, making them sufficient for most metabolic engineering applications. Using selected high-efficiency sites, we achieved simultaneous double and triple integrations and efficiently integrated long functional pathways (up to 14.7 kb). Additionally, we developed a new inducible marker recycling system that allows multiple rounds of integration at our characterized sites. We validated this system by performing five sequential rounds of GFP integration and three sequential rounds of MaFAR integration for fatty alcohol production, demonstrating, for the first time, precise gene copy number tuning in R. toruloides . These characterized integration sites should significantly advance metabolic engineering efforts and future genetic tool development in R. toruloides .

Conversion↗
DOE OSTI2021

A Scalable Process for Upcycling Carbon Dioxide (CO 2 ) and Coal Combustion Residues into Construction Products

Anthropogenic sources of carbon dioxide are generated from a number of sources, but the key among these are ordinary Portland cement (OPC) production and combustion of fossil fuels. Cement production is the largest global CO 2 source from the mineral decomposition of carbonates. Combination of the limestone decomposition and thermal requirements of the clinkering process causes cement production to contribute 8-9% of annual global CO 2 emissions. Combustion of fossil fuels (coal, oil and gas) was shown to contribute a much larger portion of global CO 2 emissions. As of 2018, combustion of fossil fuels accounted for 65% of global CO 2 , where 41% was derived from stationary sources for electricity and heat generation and the other 24% was related to transport. To reduce these contributions, key steps forward in CO 2 utilization technologies are required. The purpose of this project is to demonstrate the feasibility of the Reversa process evolving from a TRL-3 technology at the bench-scale up to TRL-6 technology at the pilot-scale. The reliability of the Reversa technology was tested to prove the effective production of concrete masonry units (CMUs) at bench scale, where the units exceeded the required 13.8 MPa compressive strength requirements. The overall goal of this project was to accelerate the development of a CO 2 mineralization process that synergistically utilizes CO 2 in flue gas and coal combustion residues (CCRs) to synthesize carbonated concrete, a functional replacement for traditional concrete. The culmination of this work resulted in 12 successful production runs at the Integrated Test Center (ITC), Gillette, WY using coal flue gas as a CO 2 source. This was followed by 6 production runs which were completed at the National Carbon Capture Center (NCCC), Wilsonville, AL, using coal-fired and natural gas (NG) flue gas as the CO 2 source. Over the course of the production runs at NCCC and ITC, the CO 2 utilization as a function of time, 24-h CO 2 uptake, electricity usage, and 28-d net area compressive strength recorded for each run. The ITC and NCCC demonstrations achieved an average of 0.19 and 0.1 g CO 2 /g reactant, respectively. Both demonstrations exceeded the project’s target goals of uptake > 0.05 g CO 2 /g reactant. Average compressive strength of the ITC blocks was 18.24 MPa at 28-days. This exceeded the target strength of 13.8 MPa specified by ASTM C90. ITC and NCCC produced an average CO 2 utilization efficiency of 74.9 and 57.9%, respectively. Both demonstration averages were within the target range of 50 to 90% utilization efficiency. For some production runs the NCCC demo did exceeded the a CO 2 utilization efficiency of 75%. An LCA of the Reversa process compared to an industry standard product revealed a net CO 2 reduction of 39% to 42%. This exceeded the target requirement of >25% net CO 2 reduction. Collection of this data was used to determine that the project was successful as the demonstration goals were achieved: (1) achieving in excess of 75% CO 2 utilization efficiency, (2) utilizing greater than 250 kg of CO 2 per production batch/run, and (3) ensuring compliance of carbonated blocks with industry standard specifications (ASTM C90).

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2021

Using Pilot Jobs and CernVM File System for Simplified Use of Containers and Software Distribution

High Energy Physics (HEP) experiments entail an abundance of computing resources, i.e. sites, to run simulations and analyses by processing data. This requirement is fulfilled by local batch farms, grid sites, private/commercial clouds, and supercomputing centers via High Throughput Computing (HTC). The growing needs of such experiments and resources being prone to trends of heterogeneity make it difficult for physicists to handle these resources directly. Additionally, HEP collaborations heavily rely on data and software releases, typically in the order of tens of gigabytes, while conducting simulations and analyses. Hence, aspects of scalability, reliability, and maintenance become crucial with regards to the distribution of the necessary data and software stack. The GlideinWMS [4] framework helps with the resource management problem by using pilot jobs, aka Glideins, to provision reliable elastic virtual clusters. Glideins are submitted to unreliable heterogeneous resources which are validated and customized by the Glideins to make the worker nodes available for end-user job execution. On the other hand, the CernVM File System (CernVM-FS or CVMFS) [1] helps with data distribution. It is a write-once, read-everywhere filesystem used to deploy scientific software to thousands of nodes on a worldwide distributed computing infrastructure. CVMFS is based on the Hyper Text Transfer Protocol and has been widely used within the particle physics community for (1) distributing experiment software and data such as calibrations, and (2) facilitating containerization by efficiently hosting container images along with providing containerization software, especially Singularity [3] GlideinWMS relies on CVMFS installed locally on the computing resources to satisfy the experiments' software needs. This requires system administrators' effort to install and maintain CVMFS at the sites and limits the use of sites, especially HPC resources, that do not have CVMFS installed. This poster presents a solution, taking advantage of Glideins to provide CVMFS at most sites without the need for a local installation. Doing so expands the pool of resources available for HEP experiments and reduces the effort of system administrators for current resources. Additionally, the proposed solution allows GlideinWMS to also start Singularity [3], a containerization software that can run unprivileged, on sites where neither CVMFS nor Singularity are available, including HPC sites. The benefits provided by this solution are: (1) lower overhead for site administrators in that they have less software to install, (2) an expanded pool of resources that run user jobs with easy access to software and data provided by CVMFS, thus making life easier for the scientists, and (3) improved flexibility to use HPC resources by enabling GlideinWMS pilot jobs to support HPC sites.

Urs, Namratha↗
DOE OSTI2022

Advancements in Yttrium Hydride Moderator Development

As microreactors evolve to become a more affordable and efficient worldwide energy source, the development of moderator material within the system to decrease the required mass of low-enriched uranium fuel is important. The use of low- instead of high-enriched uranium in small nuclear reactors stems from recent national policies associated with nonproliferation. New designs are being developed for a range of applications and nuclear space systems in particular. Using system geometries such as those described in this paper, the next step is to advance the technology readiness level of moderator material such as delta-yttrium hydride (YH x,x = 1.6–2.0 ) so that it can be qualified for use in a microreactor system. Although characterization of unirradiated material has been documented previously, to fully understand the performance of this material, behavior in relevant irradiation environments must occur. This paper describes the fabrication of yttrium hydride samples through innovative techniques and how these samples were tested in two relevant neutron environments. These two experiments include (1) a critical experiment performed at the National Criticality Experiments Research Center (NCERC) to evaluate reactivity changes in a neutron-critical environment and (2) irradiation in the Advanced Test Reactor (ATR) to assess structural integrity/material form, thermophysical data, hydrogen permeability, and other features post irradiation. For this purpose, hundreds of samples were fabricated for the NCERC and ATR experiments and are described within this paper.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗
NASA NTRS1992

Application of fiber bridging models to fatigue crack growth in unidirectional titanium matrix composites

Several fiber bridging models were reviewed and applied to study the matrix fatigue crack growth behavior in center notched (0)(sub 8) SCS-6/Ti-15-3 and (0)(sub 4) SCS-6/Ti-6Al-4V laminates. Observations revealed that fatigue damage consisted primarily of matrix cracks and fiber matrix interfacial failure in the (0)(sub 8) SCS-6/Ti-15-3 laminates. Fiber-matrix interface failure included fracture of the brittle reaction zone and cracking between the two carbon rich fiber coatings. Intact fibers in the wake of the matrix cracks reduce the stress intensity factor range. Thus, an applied stress intensity factor range is inappropriate to characterize matrix crack growth behavior. Fiber bridging models were used to determine the matrix stress intensity factor range in titanium metal matrix composites. In these models, the fibers in the wake of the crack are idealized as a closure pressure. An unknown constant frictional shear stress is assumed to act along the debond or slip length of the bridging fibers. The frictional shear stress was used as a curve fitting parameter to available data (crack growth data, crack opening displacement data, and debond length data). Large variations in the frictional shear stress required to fit the experimental data indicate that the fiber bridging models in their present form lack predictive capabilities. However, these models provide an efficient and relatively simple engineering method for conducting parametric studies of the matrix growth behavior based on constituent properties.

Bakuckas, J. G., Jr.↗