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At least 1,441 records · Page 80

Machine learning models for maintenance cost estimation in delivery trucks using diesel and natural gas fuels

The maintenance costs can represent about 15%–60% of the cost of produced goods depending on the type of goods transported. To comply with stringent emissions regulations, diesel engines are incorporated with complex after-treatment systems that demand increased maintenance. The availability of alternative fuels such as natural gas and propane has fostered the natural gas and propane powertrain systems as well as electrification options for heavy- and medium-duty vehicles. A critical barrier to adopting alternative fuel vehicles has been the lack of knowledge on comparative vehicle maintenance/repair costs with conventional diesel. Moreover, the region of operation, the type of vehicle operation, and seasonal temperature changes also affect the duty cycle which impacts the maintenance and repair costs. This study focuses on estimating the cost-per-mile for heavy-duty vehicles using machine learning models such as random forest, xgboost, neural networks, and a super-learner model. The super-learner model achieved an error as low as 0.0068 $/mile for mean absolute error and 0.0086 $/mile for root mean square error with a coefficient of determination/R-Squared of 97.28%. Specifically, the paper investigates the data collected from the maintenance and repair costs associated with delivery trucks using diesel and natural gas fuels. Since the availability of data is the major constraint, we leveraged the data collected by West Virginia University and the partnership with fleet companies. This allows for additional information related to maintenance costs and fleet-specific maintenance practices of alternative fuel vehicles. This study promotes clean fuel technologies and enables fleet management companies to adopt alternative fuel vehicles in case of similar or lower cost of maintenance compared to diesel vehicles resulting in reduced emissions and total cost of ownership.

Katreddi, Sasanka↗

The reduced genomes of Parcubacteria (OD1) contain signatures of a symbiotic lifestyle

Candidate phylum OD1 bacteria (also referred to as Parcubacteria) have been identified in broad range of anoxic environments through community survey analysis. Although none of these species have been isolated in the laboratory, several genome sequences have been reconstructed from metagenomic sequence data and single-cell sequencing. The organisms have small (generally <1 Mb) genomes with severely reduced metabolic capabilities. We have reconstructed 8 partial to near-complete OD1 genomes from oxic groundwater samples, and compared them against existing genomic data. The conserved core gene set comprises 202 genes, or ~28% of the genomic complement. ‘Housekeeping’ genes and genes for biosynthesis of peptidoglycan and Type IV pilus production are conserved. Gene sets for biosynthesis of cofactors, amino acids, nucleotides and fatty acids are absent entirely or greatly reduced. The only aspects of energy metabolism conserved are the non-oxidative branch of the pentose-phosphate shunt and central glycolysis. These organisms also lack some activities conserved in almost all other known bacterial genomes, including signal recognition particle, pseudouridine synthase A, and FAD synthase. Pan-genome analysis indicates a broad genotypic diversity and perhaps a highly fluid gene complement, indicating historical adaptation to a wide range of growth environments and a high degree of specialization. The genomes were examined for signatures suggesting either a free-living, streamlined lifestyle or a symbiotic lifestyle. The lack of biosynthetic capabilities and DNA repair, along with the presence of potential attachment and adhesion proteins suggest the Parcubacteria are ectosymbionts or parasites of other organisms. The wide diversity of genes that potentially mediate cell-cell contact suggests a broad range of partner/prey organisms across the phylum.

candidate phyla↗

Overexpression of the Mas1 gene mitigated LPS-induced inflammatory injury in mammary epithelial cells by inhibiting the NF-κB/MAPKs signaling pathways

Breast infection is the primary etiology of mastitis in dairy cows, leading to a reduction in the quality of dairy products and resulting in substantial economic losses for animal husbandry. Although antibiotic treatment can eliminate the pathogenic microorganisms that induce mastitis, it cannot repair the inflammatory damage of mammary epithelial cells and blood milk barrier. Mas1 is a G protein-coupled receptor, and its role in lipopolysaccharide (LPS) -induced inflammatory injury to mammary epithelial cells has not been studied. LPS treatment of EpH4 EV cells led to a significant downregulation of Mas1 transcript levels, which attracted our great interest, suggesting that Mas1 may be an important target for the treatment of mastitis. Therefore, this study intends to verify the role of Mas1 in the inflammatory injury of EpH4 EV cells by gene overexpression technology and gene silencing technology. The findings demonstrated that the overexpression of the Mas1 gene effectively reversed the activation of the nuclear factor-κB/mitogen-activated protein kinase (NF-κB/MAPK) signaling pathways induced by LPS, while also suppressing the upregulation of pro-inflammatory mediators. Furthermore, overexpression of the Mas1 gene reversed the downregulation of zonula occludens 1 (ZO-1), Occludin, and Claudin-3 caused by LPS, suggesting that Mas1 could promote to repair the blood-milk barrier. However, the silencing of the Mas1 gene using siRNA resulted in a contrasting effect. These results indicated that Mas1 alleviated the inflammatory injury of mammary epithelial cells induced by LPS.

Yan, Shuping↗

Lack of Genomic Instability in Bone Marrow Cells of SCID Mice Exposed Whole-Body to Low-Dose Radiation

It is clear that high-dose radiation is harmful. However, despite extensive research, assessment of potential health-risks associated with exposure to low-dose radiation (at doses below or equal to 0.1 Gy) is still challenging. Recently, we reported that 0.05 Gy of 137Cs gamma rays (the existing limit for radiation-exposure in the workplace) was incapable of inducing significant in vivo genomic instability (measured by the presence of late-occurring chromosomal damage at 6 months post-irradiation) in bone marrow (BM) cells of two mouse strains, one with constitutively high and one with intermediate levels of the repair enzyme DNA-dependent protein-kinase catalytic-subunit (DNA-PKcs). In this study, we present evidence for a lack of genomic instability in BM cells of the severely combined-immunodeficiency (SCID/J) mouse (which has an extremely low-level of DNA-PKcs activity) exposed whole-body to low-dose radiation (0.05 Gy). Together with our previous report, the data indicate that low-dose radiation (0.05 Gy) is incapable of inducing genomic instability in vivo (regardless of the levels of DNA-PKcs activity of the exposed mice), yet higher doses of radiation (0.1 and 1 Gy) do induce genomic instability in mice with intermediate and extremely low-levels of DNA-PKcs activity (indicating an important role of DNA-PKcs in DNA repair).

chromosome aberrations↗

Sediment Transport into the Swinomish Navigation Channel, Puget Sound—Habitat Restoration versus Navigation Maintenance Needs

The 11 mile (1.6 km) Swinomish Federal Navigation Channel provides a safe and short passage to fishing and recreational craft in and out of Northern Puget Sound by connecting Skagit and Padilla Bays, US State abbrev., USA. A network of dikes and jetties were constructed through the Swinomish corridor between 1893 and 1936 to improve navigation functionality. Over the years, these river training dikes and jetties designed to minimize sedimentation in the channel have deteriorated, resulting in reduced protection of the channel. The need to repair or modify dikes/jetties for channel maintenance, however, may conflict with salmon habitat restoration goals aimed at improving access, connectivity and brackish water habitat. Several restoration projects have been proposed in the Skagit delta involving breaching, lowering, or removal of dikes. To assess relative merits of the available alternatives, a hydrodynamic model of the Skagit River estuary was developed using the Finite Volume Community Ocean Model (FVCOM). Here, in this paper, we present the refinement and calibration of the model using oceanographic data collected from the years 2006 and 2009 with a focus on the sediment and brackish water transport from the river and Skagit Bay tide flats to the Swinomish Channel. The model was applied to assess the feasibility of achieving the desired dual outcome of (a) reducing sedimentation and shoaling in the Swinomish Channel and (b) providing a direct migration pathway and improved conveyance of freshwater into the Swinomish Channel. Finally, the potential reduction in shoaling through site-specific structure repairs is evaluated. Similarly, the potential to significantly improve of brackish water habitat through dike breach restoration actions using the McGlinn Causeway project example, along with its impacts on sediment deposition in the Swinomish Navigation Channel, is examined

54 ENVIRONMENTAL SCIENCES↗

Peening Techniques for Mitigating Chlorine-Induced Stress Corrosion Cracking of Dry Storage Canisters for Nuclear Applications

Fusion-welded austenitic stainless steel (ASS) was predominantly employed to manufacture dry storage canisters (DSCs) for the storage applications of spent nuclear fuel (SNF). However, the ASS weld joints are prone to chloride-induced stress corrosion cracking (CISCC), a critical safety issue in the nuclear industry. DSCs were exposed to a chloride-rich environment during storage, creating CISCC precursors. The CISCC failure leads to nuclear radiation leakage. Therefore, there is a critical need to enhance the CISCC resistance of DSC weld joints using promising repair techniques. This review article encapsulates the current state-of-the-art of peening techniques for mitigating the CISCC in DSCs. More specifically, conventional shot peening (CSP), ultrasonic impact peening (UIP), and laser shock peening (LSP) were elucidated with a focus on CISCC mitigation. The underlying mechanism of CISCC mitigation in each process was summarized. Finally, this review provides recent advances in surface modification techniques, repair techniques, and developments in welding techniques for CISCC mitigation in DSCs.

Chemistry↗

Advances in the Understanding of the Lifecycle of Photosystem II

Photosystem II is a light-driven water-plastoquinone oxidoreductase present in cyanobacteria, algae and plants. It produces molecular oxygen and protons to drive ATP synthesis, fueling life on Earth. As a multi-subunit membrane-protein-pigment complex, Photosystem II undergoes a dynamic cycle of synthesis, damage, and repair known as the Photosystem II lifecycle, to maintain a high level of photosynthetic activity at the cellular level. Cyanobacteria, oxygenic photosynthetic bacteria, are frequently used as model organisms to study oxygenic photosynthetic processes due to their ease of growth and genetic manipulation. The cyanobacterial PSII structure and function have been well-characterized, but its lifecycle is under active investigation. In this review, advances in studying the lifecycle of Photosystem II in cyanobacteria will be discussed, with a particular emphasis on new structural findings enabled by cryo-electron microscopy. These structural findings complement a rich and growing body of biochemical and molecular biology research into Photosystem II assembly and repair.

59 BASIC BIOLOGICAL SCIENCES↗

The complete structure of the human TFIIH core complex

Transcription factor IIH (TFIIH) is a heterodecameric protein complex critical for transcription initiation by RNA polymerase II and nucleotide excision DNA repair. The TFIIH core complex is sufficient for its repair functions and harbors the XPB and XPD DNA-dependent ATPase/helicase subunits, which are affected by human disease mutations. Transcription initiation additionally requires the CdK activating kinase subcomplex. Previous structural work has provided only partial insight into the architecture of TFIIH and its interactions within transcription pre-initiation complexes. Here, we present the complete structure of the human TFIIH core complex, determined by phase-plate cryo-electron microscopy at 3.7 Å resolution. The structure uncovers the molecular basis of TFIIH assembly, revealing how the recruitment of XPB by p52 depends on a pseudo-symmetric dimer of homologous domains in these two proteins. The structure also suggests a function for p62 in the regulation of XPD, and allows the mapping of previously unresolved human disease mutations.

59 BASIC BIOLOGICAL SCIENCES↗

Reliability estimation procedures and CARE: The Computer-Aided Reliability Estimation Program

Ultrareliable fault-tolerant onboard digital systems for spacecraft intended for long mission life exploration of the outer planets are under development. The design of systems involving self-repair and fault-tolerance leads to the companion problem of quantifying and evaluating the survival probability of the system for the mission under consideration and the constraints imposed upon the system. Methods have been developed to (1) model self-repair and fault-tolerant organizations; (2) compute survival probability, mean life, and many other reliability predictive functions with respect to various systems and mission parameters; (3) perform sensitivity analysis of the system with respect to mission parameters; and (4) quantitatively compare competitive fault-tolerant systems. Various measures of comparison are offered. To automate the procedures of reliability mathematical modeling and evaluation, the CARE (computer-aided reliability estimation) program was developed. CARE is an interactive program residing on the UNIVAC 1108 system, which makes the above calculations and facilitates report preparation by providing output in tabular form, graphical 2-dimensional plots, and 3-dimensional projections. The reliability estimation of fault-tolerant organization by means of the CARE program is described.

Mathur, F. P.↗

Refurbishment cost study of the thermal protection system of a space shuttle vehicle, phase 2

Labor costs and techniques associated with the refurbishment of representative TPS and their attach concepts suitable for space shuttle application have been identified on the basis of experimental tests. Ablative and reusable surface insulation TPS configurations were designed, fabricated, and tested on a full-scale mockup. The TPS attachment methods investigated included pi-strap, multiple mechanical fasteners, key/keyway, and direct bond concepts. Techniques for performing installation, inspection, repair and replacement of TPS components were studied by examining a variety of shop procedures. Major problem areas associated with these procedures and the designs to which they were applied were analyzed for several significant parameters such as handling, gaskets between joints, repair techniques, ablator plugs, etc. Results of time and motion studies of specific maintenance tasks, simulating operational procedures, were obtained. Using these data, refurbishment labor cost projections were generated for a representative Space Shuttle orbiter.

Haas, D. W.↗

Logistics hardware and services control system

Software system permits onsite direct control of logistics operations, which include spare parts, initial installation, tool control, and repairable parts status and control, through all facets of operations. System integrates logistics actions and controls receipts, issues, loans, repairs, fabrications, and modifications and assets in predicting and allocating logistics parts and services effectively.

Koromilas, A.↗

Saving Skylab

Initial difficulties encountered with the launch and operational deployment of the Skylab vehicle are described in terms of early indications of hardware failures, ground-based efforts at diagnosing the nature and significance of damage, and organization of improvized means and procedures for repair operations. Attention is given to heating and power-supply problems stemming from the loss of a micrometeoroid shield and a solar cell array. Systems engineering problems arising from the necessity of achieving a compromise among thermal, electrical, and stabilization requirements are explained, and repair solutions and operations in space and on the ground are described in detail.-

Schneider, W. C.↗

Cluster man/system design requirements and verification

Discussion of the procedures employed for determining the man/system requirements that guided Skylab design, and review of the techniques used for implementing the man/system design verification. The foremost lesson learned from the design need anticipation and design verification experience is the necessity to allow for human capabilities of in-flight maintenance and repair. It is now known that the entire program was salvaged by a series of unplanned maintenance and repair events which were implemented in spite of poor design provisions for maintenance.

Orbital workers↗

Skylab extravehicular activity

The use of extravehicular activity (EVA) techniques during the Skylab program for accomplishing major mission objectives and major and minor repair work outside the Skylab workshop is discussed. There were ten EVA periods during Skylab that lasted 82.5 man-hr. Accomplishments included those planned before the mission; but, more important, the Skylab mission was saved by EVA. The life-giving solar wing was erected during the first manned Skylab mission, and the permanent solar shield was erected during the second manned Skylab mission. In addition, 18 extra mission objectives and 13 in-flight repair tasks were accomplished through EVA during the Skylab missions.

Schultz, D. C.↗

ERTS-1 DCS technical support provided by Wallops Station

Wallops Station accepted the tasks of providing ground truth to several ERTS investigators, operating a DCP repair depot, designing and building an airborne DCP Data Acquisition System, and providing aircraft underflight support for several other investigators. Additionally, the data bank is generally available for use by ERTS and other investigators that have a scientific interest in data pertaining to the Chesapeake Bay area. Working with DCS has provided a means of evaluating the system as a data collection device possibly applicable to ongoing Earth Resources Program activities in the Chesapeake Bay area as well as providing useful data and services to other ERTS investigators. The two areas of technical support provided by Wallops, ground truth stations and repair for DCPs, are briefly discussed.

Smith, R.↗

Skylab extravehicular activity

The use of extravehicular activity (EVA) techniques during Skylab for accomplishing major mission objectives and major and minor repair work outside the Skylab workshop is discussed. The basic feasibility of EVA was demonstrated during the Gemini Program. Extravehicular activity was again demonstrated on Apollo, and was used during the Apollo 15 to 17 missions to retrieve film from the cameras used to map the lunar surface. The techniques developed for these earlier extravehicular activities were used to the fullest possible extent during Skylab. Premission plans for Skylab EVA included six EVA periods for a total of 29 man-hours. The objective of the EVA was to retrieve the Apollo telescope mount film and several particle sample collectors. There were actually 10 EVA periods during Skylab that lasted 82.5 man-hours. Accomplishments included those planned before the mission; but, more important, the Skylab mission was saved by EVA. The life-saving solar wing was erected during Skylab 2, and the permanent solar shield was erected during Skylab 3. In addition, 18 extra mission objectives and 13 in-flight repair tasks were accomplished through EVA during the Skylab missions.

Schultz, D. C.↗

Integrated orbital servicing and payloads study. Volume 1: Executive summary

A study is summarized in which a comparison was made of the following modes of maintaining a satellite system: (1) expendable mode in which failed satellites are replaced, (2) on-orbit servicing where a satellite can be fixed by unmanned module exchange in space, and (3) ground refurbishment in which the satellite is brought back to ground for repairs. It was concluded that on-orbit maintenance is the most cost-effective mode and that it is technically feasible. It can be used to repair failed satellites, to improve reliability of operating satellites, and to update equipment. On-orbit servicing can increase program flexibility and satellite reliability, lifetime, and availability. The significant conclusions and results of two studies are summarized.

Source record↗