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At least 1,441 records · Page 80
All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

Latent Neural ODE for Integrating Multi-Timescale Measurements in Smart Distribution Grids

Under a smart grid paradigm, there has been an increase in sensor installations to enhance situational awareness. The measurements from these sensors can be leveraged for real-time monitoring, control, and protection. However, these measurements are typically irregularly sampled. These measure-ments may also be intermittent due to communication bandwidth limitations. To tackle this problem, this paper proposes a novel latent neural ordinary differential equations (LODE) approach to aggregate the unevenly sampled multivariate time-series measurements. The proposed approach is flexible in performing both imputations and predictions while being computationally efficient. Simulation results on IEEE 37 bus test systems illustrate the efficiency of the proposed approach.

multi time-scale measurements↗
NASA NTRS2004

A modular suite of hardware enabling spaceflight cell culture research

BioServe Space Technologies, a NASA Research Partnership Center (RPC), has developed and operated various middeck payloads launched on 23 shuttle missions since 1991 in support of commercial space biotechnology projects. Modular cell culture systems are contained within the Commercial Generic Bioprocessing Apparatus (CGBA) suite of flight-qualified hardware, compatible with Space Shuttle, SPACEHAB, Spacelab and International Space Station (ISS) EXPRESS Rack interfaces. As part of the CGBA family, the Isothermal Containment Module (ICM) incubator provides thermal control, data acquisition and experiment manipulation capabilities, including accelerometer launch detection for automated activation and thermal profiling for culture incubation and sample preservation. The ICM can accommodate up to 8 individually controlled temperature zones. Command and telemetry capabilities allow real-time downlink of data and video permitting remote payload operation and ground control synchronization. Individual cell culture experiments can be accommodated in a variety of devices ranging from 'microgravity test tubes' or standard 100 mm Petri dishes, to complex, fed-batch bioreactors with automated culture feeding, waste removal and multiple sample draws. Up to 3 levels of containment can be achieved for chemical fixative addition, and passive gas exchange can be provided through hydrophobic membranes. Many additional options exist for designing customized hardware depending on specific science requirements.

manned↗
NASA NTRS2012

Apparatus and methods for manipulation and optimization of biological systems

The invention provides systems and methods for manipulating, e.g., optimizing and controlling, biological systems, e.g., for eliciting a more desired biological response of biological sample, such as a tissue, organ, and/or a cell. In one aspect, systems and methods of the invention operate by efficiently searching through a large parametric space of stimuli and system parameters to manipulate, control, and optimize the response of biological samples sustained in the system, e.g., a bioreactor. In alternative aspects, systems include a device for sustaining cells or tissue samples, one or more actuators for stimulating the samples via biochemical, electromagnetic, thermal, mechanical, and/or optical stimulation, one or more sensors for measuring a biological response signal of the samples resulting from the stimulation of the sample. In one aspect, the systems and methods of the invention use at least one optimization algorithm to modify the actuator's control inputs for stimulation, responsive to the sensor's output of response signals. The compositions and methods of the invention can be used, e.g., to for systems optimization of any biological manufacturing or experimental system, e.g., bioreactors for proteins, e.g., therapeutic proteins, polypeptides or peptides for vaccines, and the like, small molecules (e.g., antibiotics), polysaccharides, lipids, and the like. Another use of the apparatus and methods includes combination drug therapy, e.g. optimal drug cocktail, directed cell proliferations and differentiations, e.g. in tissue engineering, e.g. neural progenitor cells differentiation, and discovery of key parameters in complex biological systems.

Ho, Chih-Ming↗
DOE OSTI2021

A multimodal and integrated approach to interrogate human kidney biopsies with rigor and reproducibility: guidelines from the Kidney Precision Medicine Project

Comprehensive and spatially mapped molecular atlases of organs at a cellular level are a critical resource to gain insights into pathogenic mechanisms and personalized therapies for diseases. The Kidney Precision Medicine Project (KPMP) is an endeavor to generate three-dimensional (3-D) molecular atlases of healthy and diseased kidney biopsies by using multiple state-of-the-art omics and imaging technologies across several institutions. Obtaining rigorous and reproducible results from disparate methods and at different sites to interrogate biomolecules at a single-cell level or in 3-D space is a significant challenge that can be a futile exercise if not well controlled. Here we describe a “follow the tissue” pipeline for generating a reliable and authentic single-cell/region 3-D molecular atlas of human adult kidney. Our approach emphasizes quality assurance, quality control, validation, and harmonization across different omics and imaging technologies from sample procurement, processing, storage, shipping to data generation, analysis, and sharing. We established benchmarks for quality control, rigor, reproducibility, and feasibility across multiple technologies through a pilot experiment using common source tissue that was processed and analyzed at different institutions and different technologies. A peer review system was established to critically review quality control measures and the reproducibility of data generated by each technology before their being approved to interrogate clinical biopsy specimens. The process established economizes the use of valuable biopsy tissue for multiomics and imaging analysis with stringent quality control to ensure rigor and reproducibility of results and serves as a model for precision medicine projects across laboratories, institutions and consortia.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1980

Changes in plasma vasopressin during motion sickness in cats

Changes in levels of plasma vasopressin (AVP) and cortisol (C) have been shown to be correlated with motion sickness and nausea in man. As part of the research aimed at validation of the cat as an appropriate animal model for motion sickness research, levels of these hormones were investigated in the cat during motion sickness elicited by vertical linear acceleration of approximately 0.6 Hz and 1 +/- 0.6 G. In Study 1, 15 cats previously screened for susceptibility to motion sickness were prepared with indwelling jugular catheters to permit withdrawl of blood with minimal disruption of the stimulus and minimum stress to the animal. AVP and C were measured in blood samples obtained during exposure to vertical linear acceleration and during control sessions in which the animals were placed in the stationary apparatus. 10 min and 1 min prior to duration; 1, 5, 10, and 20 min after start of motion. Total duration of exposure to motion was 20 min. The data indicate that both AVP and C are elevated during exposure to motion if emesis occurs. AVP reaches maximum levels during or about the same time as emesis, while C increases gradually throughout the period of vertical acceleration. In Study 2, four cats were prepared with indwelling catheters and AVP was measured in blood withdrawn during exposure to the vertical linear acceleration. A single pre-motion sample consisting of three samples drawn 5 min prior to motion onset. Two series of samples consisting of three samples drawn at 3-min intervals were obtained during motion. The first series was initiated at emesis, and the second 25 min after emesis. Results show that levels of circulating AVP were elevated (2 to 27 times the control and pre-motion levels) in the samples taken during emesis and decreased, but remained 1 to 6 times above the pre-motion or control levels within 25 min. The results of these two studies indicate that AVP is elevated during motion-produced emesis than is C. These findings are in general agreement with those obtained from humans under motion sickness conditions, and indicate that it is appropriate to continue to use the cat in studies of hormone changes during motion sickness.

R Fox↗
NASA NTRSDate not supplied

Sampling the ENSO response to tropical volcanic eruptions in the GISS-E2-1 model: physical & biogeochemical ocean effects

Despite observational evidence that an El-Niño is likely to follow tropical volcanic eruptions (Predybaylo et al, 2017), modelling studies have so far reached no consensus on either the sign or physical mechanism of El Niño response to volcanism (Khodri et al, 2017) . A previous modelling study found that following the Pinatubo eruption, the tropical Pacific developed El Niño-like conditions that triggered a decrease in the upwelling of DIC and increased carbon uptake (Eddebbar et al., 2019). However, the carbon cycle response to volcanisms as it depends on ENSO phase remains unclear. Here we perform thirty ensemble control simulations using the GISS-E2-1-G model to sample the pre-industrial control ENSO cycle and thirty more to superimpose a Pinatubo-like volcanic eruption. We find that the model tends to favor the development of a weak El-Niño following the volcanic eruption only in the case of a volcano that erupts at the start of a neutral ENSO phase. In response to the weak El-Niño, some anomalies have the same sign as those that develop from a strong El-Niño, such as weaker winds, shallower mixed layer depths, less direct but more diffuse radiation at the surface of the ocean, less nitrate, and lower primary productivity. However, other anomalies show the opposite sign as those from a strong El-Niño, such as more precipitation, less surface DIC, and less surface ocean pCO 2 . In contrast to the neutral phase, the volcanic response during positive ENSO and negative ENSO phases are swamped by the model’s internal variability.

ENSO↗
NASA NTRS1990

Nonlinear feedback method of robot control - A preliminary experimental study

The nonlinear feedback method of robot control has been experimentally implemented on two PUMA 560 robot arms. The feasibility of the proposed controller, which was shown viable through simulation results earlier, is stressed. The servomechanism operates in task space, and the nonlinear feedback takes care of the necessary transformations to compute the necessary joint currents. A discussion is presented of the implementation with details of the experiments performed. The performance of the controller is encouraging but was limited to 100-Hz sampling frequency and to derived velocity information at the time of the experimentation. The setup of the lab, the software aspects, results, and the control hardware architecture that has recently been implemented are discussed.

Tarn, T. J.↗
NASA NTRS1998

Second Flight of the Zeno Experiment on USMP-3

The second flight of the Zeno experiment in the USMP-3 flight has shown successful control of density perturbations near the window surfaces. These density changes were demonstrated to be arising from the rate of temperature change in the sample cell during the flight. These effects could be controlled by using decreasing ramp rates as Tc was approached. The cell window interference was carefully recorded to provide data about the phase of the interference close to the critical point and the effective window reflectivity. Correlograms were recorded from 500 mili K down to 2 mili K at 24 temperatures, 383 correlograms in all. The efforts to get closer were prevented by a long (greater than two week time constant) decay of a 1.3% density error caused by local heating from the 17 micro W laser beam. The phase boundary was located with unprecedented precision of +/- 2O micro K. The complete experiment sequence was done with ground commanding and uploaded script files, bypassing the original autonomous-mode program sequence.

Gammon, Robert W.↗
NASA NTRS2011

Technical Tension Between Achieving Particulate and Molecular Organic Environmental Cleanliness: Data from Astromaterial Curation Laboratories

NASA Johnson Space Center operates clean curation facilities for Apollo lunar, Antarctic meteorite, stratospheric cosmic dust, Stardust comet and Genesis solar wind samples. Each of these collections is curated separately due unique requirements. The purpose of this abstract is to highlight the technical tensions between providing particulate cleanliness and molecular cleanliness, illustrated using data from curation laboratories. Strict control of three components are required for curating samples cleanly: a clean environment; clean containers and tools that touch samples; and use of non-shedding materials of cleanable chemistry and smooth surface finish. This abstract focuses on environmental cleanliness and the technical tension between achieving particulate and molecular cleanliness. An environment in which a sample is manipulated or stored can be a room, an enclosed glovebox (or robotic isolation chamber) or an individual sample container.

Allton, J. H.↗
DOE OSTI2025

Ultrathin VO 2 Films on Functional Substrates

The metal–insulator transition (MIT) in vanadium dioxide (VO 2 ) thin films is strongly affected by grain size, thickness, and interfacial properties. Typically, a minimum thickness around 50 nm is required for VO 2 to exhibit a significant MIT when functional substrates like sapphire and silicon are used. Several works have shown that thin films below 20 nm, with up to 2–3 decades of change in the resistance across the MIT, can be achieved but require complex pre- or postprocessing of the samples. We show that predeposition substrate condition control facilitates the direct growth of VO 2 ultrathin 15 nm films, exhibiting a resistance change between 3 and 4 decades across the MIT. Our findings indicate that the interface between the film and the substrate is crucial in determining the initial growth layers and the structural evolution. With appropriate substrate surface treatment, the desired VO 2 MIT can be enhanced regardless of the substrate crystallographic orientation. Moreover, we propose a novel approach to obtain large resistance changes across the MIT in ultrathin VO 2 films by incorporating a predeposited 1.5 nm vanadium oxide buffer layer, thereby eliminating the need to use different materials or complex pre- or postprocessing of the samples. Here we also demonstrate that this method improves the transition of 25–50 nm VO 2 thin films on silicon substrates. Our study reveals a simple approach for direct growth of ultrathin VO 2 films exhibiting a significant MIT, which is commonly accepted unattainable over substrates of technological importance, such as sapphire and silicon.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
NASA NTRS2014

Status of Propulsion Technology Development Under the NASA In-Space Propulsion Technology Program

Since 2001, the In-Space Propulsion Technology (ISPT) program has been developing and delivering in-space propulsion technologies for NASA's Science Mission Directorate (SMD). These in-space propulsion technologies are applicable, and potentially enabling for future NASA Discovery, New Frontiers, Flagship and sample return missions currently under consideration. The ISPT program is currently developing technology in three areas that include Propulsion System Technologies, Entry Vehicle Technologies, and Systems/Mission Analysis. ISPT's propulsion technologies include: 1) the 0.6-7 kW NASA's Evolutionary Xenon Thruster (NEXT) gridded ion propulsion system; 2) a 0.3-3.9kW Halleffect electric propulsion (HEP) system for low cost and sample return missions; 3) the Xenon Flow Control Module (XFCM); 4) ultra-lightweight propellant tank technologies (ULTT); and 5) propulsion technologies for a Mars Ascent Vehicle (MAV). The NEXT Long Duration Test (LDT) recently exceeded 50,000 hours of operation and 900 kg throughput, corresponding to 34.8 MN-s of total impulse delivered. The HEP system is composed of the High Voltage Hall Accelerator (HIVHAC) thruster, a power processing unit (PPU), and the XFCM. NEXT and the HIVHAC are throttle-able electric propulsion systems for planetary science missions. The XFCM and ULTT are two component technologies which being developed with nearer-term flight infusion in mind. Several of the ISPT technologies are related to sample return missions needs: MAV propulsion and electric propulsion. And finally, one focus of the Systems/Mission Analysis area is developing tools that aid the application or operation of these technologies on wide variety of mission concepts. This paper provides a brief overview of the ISPT program, describing the development status and technology infusion readiness.

systems analysis↗
NASA NTRS1960

A Homing Missile Control System to Reduce the Effects of Radome Diffraction

The problem of radome diffraction in radar-controlled homing missiles at high speeds and high altitudes is considered from the point of view of developing a control system configuration which will alleviate the deleterious effects of the diffraction. It is shown that radome diffraction is in essence a kinematic feedback of body angular velocities which causes the radar to sense large apparent line-of-sight angular velocities. The normal control system cannot distinguish between the erroneous and actual line-of-sight rates, and entirely wrong maneuvers are produced which result in large miss distances. The problem is resolved by adding to the control system a special-purpose computer which utilizes measured body angular velocity to extract from the radar output true line-of-sight information for use in steering the missile. The computer operates on the principle of sampling and storing the radar output at instants when the body angular velocity is low and using this stored information for maneuvering commands. In addition, when the angular velocity is not low the computer determines a radome diffraction compensation which is subtracted from the radar output to reduce the error in the sampled information. Analog simulation results for the proposed control system operating in a coplanar (vertical plane) attack indicate a potential decrease in miss distance to an order of magnitude below that for a conventional system. Effects of glint noise, random target maneuvers, initial heading errors, and missile maneuverability are considered in the investigation.

Smith, Gerald L.↗
NASA NTRS2002

Comparative Results of Using Different Methods for Discovery of Microorganisms in very Ancient Layers of the Central Antarctic Glacier above the Lake Vostok

The ice sheet of the Central Antarctic is considered by the scientific community worldwide, as a model to elaborate on different methods to search for life outside Earth. This became especially significant in connection with the discovery of the underglacial lake in the vicinity of the Russian Antarctic Station Vostok. Lake Vostok is considered by many scientists as an analog of the ice covered seas of Jupiter's satellite Europa. According to the opinion of many researchers there is the possibility that relict forms of microorganisms, well preserved since the Ice Age, may be present in this lake. Investigations throughout the thickness of the ice sheet above Lake Vostok show the presence of microorganisms belonging to different well-known taxonomic groups, even in the very ancient horizons near close to floor of the glacier. Different methods were used to search for microorganisms that are rarely found in the deep ancient layers of an ice sheet. The method of aseptic sampling from the ice cores and the results of controlled sterile conditions in all stages when conducting these investigations, are described in detail in previous reports. Primary investigations tried the usual methods of sowing samples onto different nutrient media, and the result was that only a few microorganisms grew on the media used. The possibility of isolating the organisms obtained for further investigations, by using modern methods including DNA-analysis, appears to be the preferred method. Further investigations of the very ancient layers of the ice sheet by radioisotopic, luminescence, and scanning electron microscopy methods at different modifications, revealed the quantity and morphological diversity of the cells of microorganisms that were distributed on the different horizons. Investigations over many years have shown that the microflora in the very ancient strata of the Antarctic ice cover, nearest to the bedrock, support the effectiveness of using a combination of different methods to search for signs of life in ancient icy formations, which might play a role in the long-term preservation and transportation of microbial life throughout the Universe.

Abyzov, S. S.↗
NASA NTRSDate not supplied

AERACEPT (Aerosol Rapid Analysis Combined Entry Probe/sonde Technology): Enabling Technology for Planetary Atmospheric Science Probes

Aerosols –clouds, hazes, and dusts –are a key part of planetary mass and energy balance, but difficult to study remotely. Current technology limits in situ measurements to once-in-a-lifetime flagship missions, which is not enough to characterize such highly dynamic systems. AERACEPT (AErosol Rapid Analysis Combined Entry Probe/sonde Technology) is an early-stage technology allowing asingle aeroshell body toact as both anentry vehicle and aerosol-samplingpassivedescent sonde, using the aeroshell’sown velocityto drive aerosol capture and separationthrough a series of embedded inlets.It takes advantage ofrecently developed thermal protection materials (3D-CC and 3MDCP)in combination withheritage aerosol sampling technologies fromboth planetary and airborne science (high-speed inlets and particle separation). By eliminating the need for heat shield separation, deployable parachutes, ordescent control, AERACEPT reducesthe mass, volume, and complexity ofplanetary aerosol sampling. Verifying AERACEPT’s performance involves modeling interdependencies between the size and geometry of the sampling inlets, the material response of the hypersonic phase of entry, the thermal conditions throughout the probe’s descent, the subsonic flow and particle sampling efficiency, the needed amount of sample, andthe cadence at which the sample can be analyzed. AERACEPT’s current predictions, using the Nephele Venus cloud mission concept (sampling between 63km to 39km, all subsonic) indicates AERACEPT can obtain >20 μL of particle material (roughly 10x the limit of detection) from droplets larger than 0.2 μm, with sample heating limited to <15 K above ambient. Validation testing for the thermal material response model has recently been performed at the UIUC Plasmatron facility, and for the flow and particle capture models is planned for this fall at the Ames Fluid Mechanics Laboratory.

AERACEPT↗
NASA NTRS1968

Preparing rock powder specimens of controlled size distribution

Apparatus produces rock powder specimens of the size distribution needed in geological sampling. By cutting grooves in the surface of the rock sample and then by milling these shallow, parallel ridges, the powder specimen is produced. Particle size distribution is controlled by changing the height and width of ridges.

Blum, P.↗
NASA NTRS1993

SALI chemical analysis of provided samples

SRI has completed the chemical analysis of all the samples supplied by NASA. The final batch of four samples consisted of: one inch diameter MgF2 mirror, control 1200-ID-FL3; one inch diameter neat resin, PMR-15, AO171-IV-55, half exposed and half unexposed; one inch diameter chromic acid anodized, EOIM-3 120-47 aluminum disc; and AO-exposed and unexposed samples of fullerene extract material in powdered form, pressed into In foil for analysis. Chemical analyses of the surfaces were performed by the surface analysis by laser ionization (SALI) method. The analyses emphasize surface contamination or general organic composition. SALI uses nonselective photoionization of sputtered or desorbed atoms and molecules above but close (approximately one mm) to the surface, followed by time-of-flight (TOF) mass spectrometry. In these studies, we used laser-induced desorption by 5-ns pulse-width 355-nm light (10-100 mJ/sq cm) and single-photon ionization (SPI) by coherent 118-nm radiation (at approximately 5 x 10(exp 5) W/sq cm). SPI was chosen primarily for its ability to obtain molecular information, whereas multiphoton ionization (not used in the present studies) is intended primarily for elemental and small molecule information. In addition to these four samples, the Au mirror (EOIM-3 200-11, sample four) was depth profiled again. Argon ion sputtering was used together with photoionization with intense 355-nm radiation (35-ps pulsewidths). Depth profiles are similar to those reported earlier, showing reproducibility. No chromium was found in the sample above noise level; its presence could at most be at the trace level. Somewhat more Ni appears to be present in the Au layer in the unexposed side, indicating thermal diffusion without chemical enhancement. The result of the presence of oxygen is apparently to tie-up/draw out the Ni as an oxide at the surface. The exposed region has a brownish tint appearance to the naked eye.

Becker, Christopher H.↗
NASA NTRS2001

Visualization of Microbial Biomarkers by Scanning Electron Microscopy

We are developing tools to link the biochemical structure of selected biomarkers with putative biogenic structures observed in mineralized samples. The detection of evidence of life on Mars and other planets will rely on methods that can discriminate compounds formed exclusively by living organisms. While biogenic compounds, such as amino acids and nucleotides have been discovered in extraterrestrial sources, such as meteorites and comets, their formation can be explained by abiotic means. The formation of cellular structures, or more elaborate organic molecules, such as complex lipids, proteins or nucleic acids, however, is strongly correlated to the presence of even the most primitive life processes. Recent evidence lends support to the hypothesis that life may have once existed on Mars. Carbonate globules and ppm concentrations of polycyclic aromatic hydrocarbons (PAHs) have been described in ALH84001, a meteorite originating from Mars ejecta captured by Earth over 13,000 years ago. The localized high concentration of PAHs that follow an increasing gradient from the intact fusion crust towards the interior corresponds to microgram quantities of hydrocarbon. Even though ALH84001 and other similar meteorites have withstood the forces capable of ejecting rock through Mars' escape velocity, upon entering Earth's atmosphere, their core temperatures are likely not to have been raised significantly, as evidenced by the survival of remanent magnetic signatures. Ideal biomarkers of ancient or modern biological life would include molecules that are (or were) pervasive and highly resistant to degradation. Also, requisite methods of detection should be simple, extremely sensitive and broadly inclusive (NASA SP-530). Lipopolysaccharide (LPS), peptidoglycan or pseudopeptidoglycan and beta-glucan are microbial cell wall components which together cover the entire microbial spectrum of eubacteria, archea and fungi. They are all remarkably resistant to thermal degradation. Fortunately, many antimicrobial defense systems of higher organisms require sensitive detection to combat microbial pathogens. We employ here the primitive immune system of the evolutionarily ancient horseshoe crab, Limulus polyphemus. This species relies on multi-enzyme signal amplification detection of cell wall molecules and they can be applied to the development of useful detectors of life. An extension of this work includes the visualization of microbial signatures by labeling LAL components with chromogenic or electron dense markers. The protein Limulus Anti-LPS Factor (LALF) has an extremely high affinity for LPS. By coupling LALF binding with colloidal gold labels we demonstrate a correlation of the structures visible by electron microscopy with biochemical evidence of microbial cell wall materials. Pure silica particles were mixed with cultures of E. coli (10(exp 6) cfu/mL). Samples were washed sequentially with buffered saline, LALF, antibody to LALF and finally colloidal gold-labeled Protein A. Negative controls were not exposed to E. coli but received identical treatment otherwise. Samples were coated with carbon and imaged on a JEOL JSM-840 scanning electron microscope with LaB6 source in the back scatter mode with the JEOL annular back scatter detector. 20 nm-scale black spots in this contrast-reversed image originate from electrons back-scattered by gold atoms. Negative controls did not give any signal. Future work will expand application of this technique to soil simulants and mineralized rock samples.

Wainwright, Norman R.↗
NASA NTRS1990

Small gain robustness issues in the p-integrator repetitive controller

The basic theory of the digital p-integrator controller is utilized to modify the command to a stable analog feedback control system in order to produce zero tracking error of a repetitive command. Mapping of continuous time poles and zeros to the images in the discrete time domain is performed. It is shown that for a pole excess of one, with p odd, the p-integrator learning controller will often produce a stable learning process, even for relatively large sample times. For a pole excess of two, a stable learning process can often be achieved for any p as long as the sample time is kept sufficiently short. Therefore, when the pole excess is zero and one utilizes the proper p-integrator controller with p odd, stable performance is often produced, even for relatively large sample times.

Longman, Richard W.↗