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At least 145 records · Page 8

Impact of irradiance and inorganic carbon availability on heterologous sucrose production in Synechococcus elongatus PCC 7942

Cyanobacteria have been proposed as a potential alternative carbohydrate feedstock and multiple species have been successfully engineered to secrete fermentable sugars. To date, the most productive cyanobacterial strains are those designed to secrete sucrose, yet there exist considerable differences in reported productivities across different model species and laboratories. In this study, we investigate how cultivation conditions (specifically, irradiance, CO 2 , and cultivator type) affect the productivity of sucrose-secreting Synechococcus elongatus PCC 7942. We find that S. elongatus produces the highest sucrose yield in irradiances far greater than what is often experimentally utilized, and that high light intensities are tolerated by S. elongatus , especially under higher density cultivation where turbidity may attenuate the effective light experienced in the culture. By increasing light and inorganic carbon availability, S. elongatus cscB / sps produced a total of 3.8 g L -1 of sucrose and the highest productivity within that period being 47.8 mg L -1 h -1 . This study provides quantitative description of the impact of culture conditions on cyanobacteria-derived sucrose that may assist to standardize cross-laboratory comparisons and demonstrates a significant capacity to improve productivity via optimizing cultivation conditions.

59 BASIC BIOLOGICAL SCIENCES↗

Methanogenesis and Salt Tolerance Genes of a Novel Halophilic Methanosarcinaceae Metagenome-Assembled Genome from a Former Solar Saltern

Anaerobic archaeal methanogens are key players in the global carbon cycle due to their role in the final stages of organic matter decomposition in anaerobic environments such as wetland sediments. Here we present the first draft metagenome-assembled genome (MAG) sequence of an unclassified Methanosarcinaceae methanogen phylogenetically placed adjacent to the Methanolobus and Methanomethylovorans genera that appears to be a distinct genus and species. The genome is derived from sediments of a hypersaline (97–148 ppt chloride) unrestored industrial saltern that has been observed to be a significant methane source. The source sediment is more saline than previous sources of Methanolobus and Methanomethylovorans. We propose a new genus name, Methanosalis, to house this genome, which we designate with the strain name SBSPR1A. The MAG was binned with CONCOCT and then improved via scaffold extension and reassembly. The genome contains pathways for methylotrophic methanogenesis from trimethylamine and dimethylamine, as well as genes for the synthesis and transport of compatible solutes. Some genes involved in acetoclastic and hydrogenotrophic methanogenesis are present, but those pathways appear incomplete in the genome. The MAG was more abundant in two former industrial salterns than in a nearby reference wetland and a restored wetland, both of which have much lower salinity levels, as well as significantly lower methane emissions than the salterns.

59 BASIC BIOLOGICAL SCIENCES↗

Graphite/polyimide tension tests at elevated and cryogenic temperatures

Interim results for characterizing tension properties of graphite/polyimide laminates are reported. Existing test procedures for resin-matrix composites were evaluated for use through the extreme temperature range indicated above. Of particular concern was the performance of resistance strain gages at the elevated temperatures and tests were carried out to compare their performance against that of a capacitive strain gage. Limited design data are reported for graphite/polyimide laminates which are being produced through the CASTS Project.

Chapman, A. J.↗

Flight set 360L003 instrumentation final test report, volume 9

Post-flight instrumentation hardware and data evaluation for 360L003 is summarized. The 360L003 motors were equipped with Developmental Flight Instrumentation (DFI), Operational Flight Instrumentation (OFI), and Ground Environmental Instrumentation (GEI). The DFI was designed to measure strain, temperature, pressure, and vibration at various locations on the motor during flight. The DFI is used to validate engineering models in a flight environment. The OFI consists of six Operational Pressure Tranducers which monitor chamber pressure during flight. These pressure transducers are used in the SRB separation cue. GEI measures the motor case, igniter flange, and nozzle temperature prior to launch.

Source record↗

National Transonic Facility model and model support vibration problems

Vibrations of models and model support system were encountered during testing in the National Transonic Facility. Model support system yaw plane vibrations have resulted in model strain gage balance design load limits being reached. These high levels of vibrations resulted in limited aerodynamic testing for several wind tunnel models. The yaw vibration problem was the subject of an intensive experimental and analytical investigation which identified the primary source of the yaw excitation and resulted in attenuation of the yaw oscillations to acceptable levels. This paper presents the principal results of analyses and experimental investigation of the yaw plane vibration problems. Also, an overview of plans for development and installation of a permanent model system dynamic and aeroelastic response measurement and monitoring system for the National Transonic Facility is presented.

Young, Clarence P., Jr.↗

Summary Report of the First International Symposium on Strain Gauge Balances and Workshop on AoA/Model Deformation Measurement Techniques

The first International Symposium on Strain Gauge Balances was sponsored under the auspices of the NASA Langley Research Center (LaRC), Hampton, Virginia. Held at the LaRC Reid Conference Center, the Symposium provided an open international forum for presentation, discussion, and exchange of technical information among wind tunnel test technique specialists and strain gauge balance designers. The Symposium also served to initiate organized professional activities among the participating and relevant international technical communities. The program included a panel discussion, technical paper sessions, tours of local facilities, and vendor exhibits. Over 130 delegates were in attendance from 15 countries. A steering committee was formed to plan a second international balance symposium tentatively scheduled to be hosted in the United Kingdom in 1998 or 1999. The Balance Symposium was followed bv the half-day, Workshop on Angle of Attack and Model Deformation on the afternoon of October 25. The thrust of the Workshop was to assess the state of the art in angle of attack (AoA) and model deformation measurement techniques and to discuss future developments.

Tripp, John S.↗

Summary Report of the First International Symposium on Strain Gauge Balances and Workshop on AoA/Model Deformation Measurement Techniques

The first International Symposium on Strain Gauge Balances was sponsored under the auspices of the NASA Langley Research Center (LaRC), Hampton, Virginia during October 22-25, 1996. Held at the LaRC Reid Conference Center, the Symposium provided an open international forum for presentation, discussion, and exchange of technical information among wind tunnel test technique specialists and strain gauge balance designers. The Symposium also served to initiate organized professional activities among the participating and relevant international technical communities. The program included a panel discussion, technical paper sessions, tours of local facilities, and vendor exhibits. Over 130 delegates were in attendance from 15 countries. A steering committee was formed to plan a second international balance symposium tentatively scheduled to be hosted in the United Kingdom in 1998 or 1999. The Balance Symposium was followed by the half-day Workshop on Angle of Attack and Model Deformation on the afternoon of October 25. The thrust of the Workshop was to assess the state of the art in angle of attack (AoA) and model deformation measurement techniques and to discuss future developments.

Tripp, John S.↗

BioHydrogen (BioH2) Consortium to Advance Fermentative H2 Production

The overall objective of this project is to develop a carbon-neutral, microbial dark fermentation technology to convert waste lignocellulosic biomass into H2 with a production cost less than $2/kg-H2 via strain engineering, bioprocess design for scale-up, and integrating fermentation with microbial electrolysis cell (MEC).

bioH2↗

BioHydrogen (BioH2) Consortium to Advance Fermentative H2 Production

The overall objective of this project is to develop a carbon-neutral, microbial dark fermentation technology to convert waste lignocellulosic biomass into H2 with a production cost less than $2/kg-H2 via strain engineering, bioprocess design for scale-up, and integrating fermentation with microbial electrolysis cell (MEC).

bioH2↗

The MISSE 7 Flexural Stress Effects Experiment After 1.5 Years of Wake Space Exposure

Low Earth orbit space environment conditions, including ultraviolet radiation, thermal cycling, and atomic oxygen exposure, can cause degradation of exterior spacecraft materials over time. Radiation and thermal exposure often results in bond- breaking and embrittlement of polymers, reducing mechanical strength and structural integrity. An experiment called the Flexural Stress Effects Experiment (FSEE) was flown with the objective of determining the role of space environmental exposure on the degradation of polymers under flexural stress. The FSEE samples were flown in the wake orientation on the exterior of International Space Station for 1.5 years. Twenty-four samples were flown: 12 bent over a 0.375 in. mandrel and 12 were over a 0.25 in. mandrel. This was designed to simulate flight configurations of insulation blankets on spacecraft. The samples consisted of assorted polyimide and fluorinated polymers with various coatings. Half the samples were designated for bend testing and the other half will be tensile tested. A non-standard bend-test procedure was designed to determine the surface strain at which embrittled polymers crack. All ten samples designated for bend testing have been tested. None of the control samples' polymers cracked, even under surface strains up to 19.7%, although one coating cracked. Of the ten flight samples tested, seven show increased embrittlement through bend-test induced cracking at surface strains from 0.70%to 11.73%. These results show that most of the tested polymers are embrittled due to space exposure, when compared to their control samples. Determination of the extent of space induced embrittlement of polymers is important for designing durable spacecraft.

Snow, Kate E.↗

Forming blocks speed production of strain gage grids

A tool is designed which facilitates the forming of wire grids used in manufacturing strain gage grids. Flattening the grid wire by a cold working process produces a stabilized grid which can be readily handled for storage or shipment.

Bonn, J. L.↗

Development of a fiber optic high temperature strain sensor

From 1 Apr. 1991 to 31 Aug. 1992, the Georgia Tech Research Institute conducted a research program to develop a high temperature fiber optic strain sensor as part of a measurement program for the space shuttle booster rocket motor. The major objectives of this program were divided into four tasks. Under Task 1, the literature on high-temperature fiber optic strain sensors was reviewed. Task 2 addressed the design and fabrication of the strain sensor. Tests and calibration were conducted under Task 3, and Task 4 was to generate recommendations for a follow-on study of a distributed strain sensor. Task 4 was submitted to NASA as a separate proposal.

Rausch, E. O.↗

An extrapolation method for strain ranges and hold times in developing the EPP+SMT creep-fatigue design curves for Alloy 617

Experimental and numerical studies in developing the integrated Elastic–Perfectly Plastic (EPP) plus Simplified Model Test (SMT) design methodology, referred to as the EPP+SMT method, continued in FY2022. This report focuses on the methods for extrapolating the EPP+SMT creep-fatigue (CF) design curves at long hold times and low strain ranges. In this study, the available CF failure data on Alloy 617 at 950°C were analyzed to determine a set of CF failure criteria. At very low strain ranges and long hold times, CF failure data are not accessible by experiments because of the extraordinarily long test durations and the inability of the test machines to accurately control these small strain ranges. A CF experimental approach with the concept of block-strain range CF testing protocol was developed. Tests using this protocol were conducted to generate the needed information for calibrating material parameters of the numerical material models. The Time Fraction based method and Dissipated Energy method were used to extrapolate the CF life curves to low strain ranges and long hold times. Based on the new experimental approach and CF life prediction methods, the CF life curves with various hold times were developed for Alloy 617 at 950°C. In addition, an experiment was designed and is being performed to verify the predicted CF curves at 950°C. The extrapolation procedure will be applied at lower temperatures to complete the development of the EPP+SMT CF design curves for Alloy 617 in F2023.

36 MATERIALS SCIENCE↗

Design and Evaluation of a Bolted Joint for a Discrete Carbon-Epoxy Rod-Reinforced Hat Section

The use of pre-fabricated pultruded carbon-epoxy rods has reduced the manufacturing complexity and costs of stiffened composite panels while increasing the damage tolerance of the panels. However, repairability of these highly efficient discrete stiffeners has been a concern. Design, analysis, and test results are presented in this paper for a bolted-joint repair for the pultruded rod concept that is capable of efficiently transferring axial loads in a hat-section stiffener on the upper skin segment of a heavily loaded aircraft wing component. A tension and a compression joint design were evaluated. The tension joint design achieved approximately 1.0 percent strain in the carbon-epoxy rod-reinforced hat-section and failed in a metal fitting at 166 percent of the design ultimate load. The compression joint design failed in the carbon-epoxy rod-reinforced hat-section test specimen area at approximately 0.7 percent strain and at 110 percent of the design ultimate load. This strain level of 0.7 percent in compression is similar to the failure strain observed in previously reported carbon-epoxy rod-reinforced hat-section column tests.

Baker, Donald J.↗

RETSCP: A computer program for analysis of rocket engine thermal strains with cyclic plasticity

A computer program, designated RETSCP, for the analysis of Rocket Engine Thermal Strain with Cyclic Plasticity is described. RETSCP is a finite element program which employs a three dimensional isoparametric element. The program treats elasto-plastic strain cycling including the effects of thermal and pressure loads and temperature dependent material properties. Theoretical aspects of the finite element method are discussed and the program logic is described. A RETSCP User's Manual is presented including sample case results.

Miller, R. W.↗

Investigation of the Leak Response of a Carbon-Fiber Laminate Loaded in Biaxial Tension

Designers of pressurized structures have been reluctant to use composite materials because of concerns over leakage. Biaxial stress states are expected to be the worst-case loading condition for allowing leakage to occur through microcracks. To investigate the leakage behavior under in-plane biaxial loading, a cruciform composite specimen was designed that would have a relatively large test section with a uniform 1:1 biaxial loading ratio. A 7.6-cm-square test section was desired for future investigations of the leakage response as a result of impact damage. Many iterations of the cruciform specimen were evaluated using finite element analysis to reduce stress concentrations and maximize the size of the uniform biaxial strain field. The final design allowed the specimen to go to relatively high biaxial strain levels without incurring damage away from the test section. The specimen was designed and manufactured using carbon/epoxy fabric with a four-ply-thick, quasi-isotropic, central test section. Initial validation and testing were performed on a specimen without impact damage. The specimen was tested to maximum biaxial strains of approximately 4500micro epsilon without apparent damage. A leak measurement system containing a pressurized cavity was clamped to the test section and used to measure the flow rate through the specimen. The leakage behavior of the specimen was investigated for pressure differences up to 172 kPa

Jackson, Wade C.↗

Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: ‘transgenic oilcane’

Sugarcane plant engineered to accumulate lipids in its vegetative tissue is being developed as a new bioenergy crop. The new crop would be a source of juice, oil, and cellulosic sugars. However, limited tolerance of industrially recognized yeasts towards inhibitors generated during the processing of lignocellulosic biomass to produce fermentable sugars is a major challenge in developing scalable processes for second-generation drop-in fuel production. To this end, hydrolysates generated from engineered sugarcane—‘oilcane’ bagasse contain added phenolics and fatty acids that further restrict the growth of fermenting microorganisms and necessitate nutrient supplementation and/or detoxification of hydrolysate which makes the fermentation process expensive. Herein, we propose a resourceful and economical approach for growing lab and commercial strains of S. cerevisiae on unrefined cellulosic sugars aerobically and fermentatively.

09 BIOMASS FUELS↗