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At least 145 records · Page 8

Energetic electrons, Type III radio bursts, and impulsive solar flare X-rays

Observations of impulsive hard X-ray and type III radio bursts made during the maximum of the last solar activity cycle are analyzed. Spectral measurements of 10-68 keV X-rays were made with the University of California (Berkeley) experiment aboard the OGO 5 satellite. About 20% of impulsive hard X-ray bursts are correlated with type III radio bursts, whereas only about 3% of the reported type III radio bursts are correlated with impulsive X-ray bursts. The location of the associated H gamma flare on the solar disk has little effect on the X-ray-type III burst correlation. The magnitude of the X-ray-type III burst correlation increases systematically with an increase in the intensity and starting frequency of the radio burst, the peak energy and hardness of the X-ray burst, and the peak nonthermal emission measure and spectral hardness of the electron spectrum not less than 20 keV inside the X-ray source. Observations are consistent with the electron populations responsible for both the X-ray and type III emissions accelerated in a single acceleration process; they also suggest a flare model where the primary instability causing electron acceleration during the impulsive phase occurs in the corona.

Kane, S. R.↗

Optimal sampling theory and population modelling - Application to determination of the influence of the microgravity environment on drug distribution and elimination

The optimal sampling theory is evaluated in applications to studies related to the distribution and elimination of several drugs (including ceftazidime, piperacillin, and ciprofloxacin), using the SAMPLE module of the ADAPT II package of programs developed by D'Argenio and Schumitzky (1979, 1988) and comparing the pharmacokinetic parameter values with results obtained by traditional ten-sample design. The impact of the use of optimal sampling was demonstrated in conjunction with NONMEM (Sheiner et al., 1977) approach, in which the population is taken as the unit of analysis, allowing even fragmentary patient data sets to contribute to population parameter estimates. It is shown that this technique is applicable in both the single-dose and the multiple-dose environments. The ability to study real patients made it possible to show that there was a bimodal distribution in ciprofloxacin nonrenal clearance.

Drusano, George L.↗

A photometric study of Saturn's F Ring

The Saturn F Ring's radially integrated brightness has been measured over a wide range of phase angles from Voyager images; in order to model the resultant phase curve, the ring population has been divided into a dust regime and one of larger bodies, and while single scattering properties of small particles are modeled by semiempirical nonspherical/randomly oriented particles, those of large bodies are based on the photometric behavior of satellites. It is suggested that the dust in the envelope arises from micrometeoroid impacts into the large core particles, and then migrates inward.

Showalter, Mark R.↗

Multispectral Imaging Broadens Cellular Analysis

Amnis Corporation, a Seattle-based biotechnology company, developed ImageStream to produce sensitive fluorescence images of cells in flow. The company responded to an SBIR solicitation from Ames Research Center, and proposed to evaluate several methods of extending the depth of field for its ImageStream system and implement the best as an upgrade to its commercial products. This would allow users to view whole cells at the same time, rather than just one section of each cell. Through Phase I and II SBIR contracts, Ames provided Amnis the funding the company needed to develop this extended functionality. For NASA, the resulting high-speed image flow cytometry process made its way into Medusa, a life-detection instrument built to collect, store, and analyze sample organisms from erupting hydrothermal vents, and has the potential to benefit space flight health monitoring. On the commercial end, Amnis has implemented the process in ImageStream, combining high-resolution microscopy and flow cytometry in a single instrument, giving researchers the power to conduct quantitative analyses of individual cells and cell populations at the same time, in the same experiment. ImageStream is also built for many other applications, including cell signaling and pathway analysis; classification and characterization of peripheral blood mononuclear cell populations; quantitative morphology; apoptosis (cell death) assays; gene expression analysis; analysis of cell conjugates; molecular distribution; and receptor mapping and distribution.

Source record↗

Two-Dimensional Planetary Surface Landers

We proposed to develop a new landing approach that significantly reduces development time and obviates the most complicated, most expensive, and highest-risk phase of a landing mission. The concept is a blanket- or carpet-like two-dimensional (2D) lander (~1-m × 1-m surface area and <1-cm thick) with a low mass/drag ratio, which allows the lander to efficiently shed its approach velocity and provide a more robust structure for landing integrity. The form factor of these landers allows dozens to be stacked on a single spacecraft for transport and distributed en masse to the surface. Lander surfaces will be populated on both sides by surface-mount, low-profile sensors and instruments, surface-mount telecom, solar cells, batteries, processors, and memory. Landers will also incorporate thin flexible electronics, made possible in part by printable electronics technology. The mass and size of these highly capable technologies further reduces the required stiffness and mass of the lander structures to the point that compliant, lightweight, robust landers capable of passive landings are possible. This capability avoids the costly, complex use of rockets, radar, and associated structure and control systems. This approach is expected to provide an unprecedented science payload mass to spacecraft mass ratio of approximately 80% (estimated based on current knowledge). This compared to ~1% for Pathfinder, ~17% for MER, and 22% for MSL rovers. Clearly, one difference is rovers vs. a lower capability lander. An outcome of the Phase I study is a clear roadmap for near-term demonstration and long-term technology development.

Landers↗

Studies of Vestibular Neurons in Normal, Hyper- and Hypogravity

During the past year, pre-, in- and postflight studies were conducted in association with the Axon project for Bion 10 (Cosmos 2229). Recordings were made during pre- and postflight studies, from 118 horizontal semicircular canal afferents and 27 vestibular nucleus neurons in 7 rhesus monkeys; 137 pulse rotation protocols alone were executed (548 acceleration and deceleration responses were curve fit). Usable data was obtained from 127 horizontal afferents concerning their spontaneous discharge. Curve fits and analysis was made of sinusoidal and sum of sinusoidal responses from 42 and 35 horizontal afferents, respectively. Also recordings were made from neurons inflight from the two flight animals. The mean spontaneous rate varied from 128 spikes/sec. during preflight to 92 spikes/sec during postflight (day 5) - a change of 28%. In direct contrast to the results of Cosmos 2044, the best fitted neural adaptation operator (k) and the gain of the pulse response were decreased during post flight when compared to preflight. Surprisingly, the best fitted gain and k values for the sum of sines were slightly elevated during post flight tests. The gain and phase of single sine responses were compared for pre- and post flight tests and compared to a larger population of afferents. In contrast to Cosmos 2044 results where on the first day of post flight testing the gains of the best fitted sine response were skewed toward the higher values of the Miles and Braitman distribution, the gain of the best fitted sine responses during the first day of post flight testing (day 2) during Cosmos 2229 were exactly on the mode of the Miles and Braitman distribution. Thus, at least for the periodic stimuli, (pulses and sine waves) we found no change in gain and neural adaptation during post flight testing following Cosmos 2229. This conclusion is different from the one derived following the Cosmos 2044 flight. Cosmos flight 2229 differed from Cosmos flight 2044 in several significant ways: For example, during preflight, (1) The animals preflight training was different (less well trained on the gaze task) and (2) the animals were exposed to more experimental manipulations (surgical and rotational). Inflight, (1) the animals were required to make a pointing gesture (motor response) in association with eye movements to obtain reward, (2) the inflight diet was different (more balanced), (3) the feeder for one of the animals clogged following 9 days of flight resulting in evident dehydration and probably less head motion exposure in that monkey and (4) there was limited video taping of the monkeys in space. During postflight, (1) we were unable to test the flight animals until 26 hours postflight as compared to 14.5 hours during Cosmos 2044, (2) the animals received significantly more exposure to motion stimuli during postflight testing than during Cosmos 2044. These differences in the vestibular environment will require analysis of several parameters other than just neural and eye movement responses. For example, computer programs will have to be written and used to recover and quantify the number of head movements made by each animal during flight. This activity is critical to the production of neural adaptation and increased gain.

Correia, Manning J.↗

Titanospirillum velox: a huge, speedy, sulfur-storing spirillum from Ebro Delta microbial mats

A long (20-30 micrometer), wide (3-5 micrometer) microbial-mat bacterium from the Ebro Delta (Tarragona, Spain) was grown in mixed culture and videographed live. Intracellular elemental sulfur globules and unique cell termini were observed in scanning-electron-microprobe and transmission-electron micrographs. A polar organelle underlies bundles of greater than 60 flagella at each indented terminus. These Gram-negative bacteria bend, flex, and swim in a spiral fashion; they translate at speeds greater than 10 body lengths per second. The large size of the spirillum permits direct observation of cell motility in single individual bacteria. After desiccation (i.e., absence of standing water for at least 24 h), large populations developed in mat samples remoistened with sea water. Ultrastructural observations reveal abundant large sulfur globules irregularly distributed in the cytoplasm. A multilayered cell wall, pliable and elastic yet rigid, distends around the sulfur globules. Details of the wall, multiflagellated termini, and large cytoplasmic sulfur globules indicate that these fast-moving spirilla are distinctive enough to warrant a genus and species designation: Titanospirillum velox genus nov., sp. nov. The same collection techniques at a similar habitat in the United States (Plum Island, northeast Essex County, Massachusetts) also yielded large populations of the bacterium among purple phototrophic and other inhabitants of sulfurous microbial-mat muds. The months-long survival of T. velox from Spain and from the United States in closed jars filled with mud taken from both localities leads us to infer that this large spirillum has a cosmopolitan distribution.

Non-NASA Center↗

Post-AGB Stars in Nearby Galaxies as Calibrators for HST

This report summarizes activities carried out with support from the NASA Ultraviolet, Visible, and Gravitational Astrophysics Research and Analysis Program under Grant NAG 5-6821. The Principal Investigator is Howard E. Bond (Space Telescope Science Institute). STScI Postdoctoral Associates Laura K. Fullton (1998), David Alves (1998-2001), and Michael Siegel (2001) were partially supported by this grant. The aim of the program is to calibrate the absolute magnitudes of post-asymptotic- giant-branch (post-AGB or PAGB) stars, which we believe will be an excellent new "standard candle" for measuring extragalactic distances. The argument is that, in old populations, the stars that are evolving through the PAGB region of the HR diagram arise from only a single main-sequence turnoff mass. In addition, theoretical PAGB evolutionary tracks show that they evolve through this region at constant luminosity; hence the PAGB stars should have an extremely narrow luminosity function. Moreover, as the PAGB stars evolve through spectral types F and A (en route from the AGB to hot stellar remnants and white dwarfs), they have the highest luminosities attained by old stars (both bolometrically and in the visual band). Finally, PAGB stars of these spectral types are very easily identified. because of their large Balmer jumps, which are due to their very low surface gravities. Our approach is first to identify PAGB stars in Milky Way globular clusters and in other Local Group galaxies, which are at known distances, and thus to measure accurate absolute magnitudes for the PAGB stars. With this Milky Way and Local Group luminosity calibration, we will then be in a position to find PAGB stars in more distant galaxies from the ground, and ultimately from the Hubble Space Telescope. and thus derive distances. These PAGB stars are, as noted above, the visually brightest members of Population II, and hence will allow distance measurements to galaxies that do not contain Cepheids, such as elliptical galaxies, as well as distances to spirals using PAGB stars in their halos. Moreover, the method is entirely independent of Cepheids. and thus provides a direct test of the Cepheid distance scale. The program will also provide information on the evolutionary lifetimes of PAGB stars.

Bond, Howard E.↗

Constraints on the formation history of the elliptical galaxy NGC 3923 from the colors of its globular clusters

We present a study of the colors of globular clusters associated with the elliptical galaxy NGC 3923. Our final sample consists of Wasington system C and T(sub 1) photometry for 143 globular cluster candidates with an expected contamination of no more than 10%. We find that the color distribution of the NGC 3923 globular cluster system (GCS) is broad and appears to have at least two peaks. A mixture modeling analysis of the color distribution indicates that a two-component model is favored over a single-component one at a high level of confidence (greater than 99%). This evidence for more than one population in the GCS of NGC 3923 is similar to that previously noted for the four other elliptical galaxies for which similar data have been published. Furthermore, we find that the NGC 3923 GCS is redder than the GCSs of previously studed elliptical galaxies of similar luminosity. The median metallicity inferred from our (C-(T(sub 1)))(sub 0) colors is (Fe/H)(sub med) = -0.56, with an uncertainty of 0.14 dex arising from all sources of uncertainty in the mean color. This is more metal rich than the median metallicity found for the GCS of M87 using the same method, (Fe/H)(sub med) = -0.94. Since M87 is more luminous than NGC 3923, this result points to significant scatter about any trend of higher GCS metallicity with increasing galaxy luminosity. We also show that there is a color gradient in the NGC 3923 GCS corresponding to about -0.5 dex in Delta(Fe/H)/Delta(log r). We conclude that the shape of the color distribution of individual GCSs and the variation in mean color among the GCSs of ellipticals are difficult to understand if elliptical galaxies are formed in a single protogalactic collapse. Models in which ellipticals and their globular clusters are formed in more than one event, such as a merger scenario, are more successful in accounting for these observations.

Zepf, Stephen E.↗

Hydride structures in Ti-aluminides subjected to high temperature and hydrogen pressure charging conditions

The distribution and chemistry of hydrides produced in single and dual phase alloys with a composition near TiAl have been investigated by using a combination of TEM and X-ray diffraction techniques. The alloys were exposed at 650 C to 13.8 MPa of gaseous H2 for 100 h. In the single-phase gamma alloy, large hydrides preferentially nucleated on the grain boundaries and matrix dislocations and a population of small hydrides was distributed throughout the matrix. X-ray and electron diffraction patterns from these hydrides indicated that they have an fcc structure with a lattice parameter of 0.45 nm. EDAX analysis of the hydrides showed that they were enriched in Ti. The hydrides were mostly removed by vacuum annealing at 800 C for 24 h. On dissolution of the hydrides, the chemistry of hydride-free regions of the grain boundary returned to the matrix composition, suggesting that Ti segregation accompanied the hydride formation rather than Ti enrichment causing the formation of the hydride.

Legzdina, D.↗

CCD photometry in the globular cluster NGC 288. I - Blue stragglers and main-sequence binary stars

Photometry based on a mosaic of CCD images in B and V is presented for the globular cluster NGC 288. The spatial coverage ranges from the cluster core to about 6 core radii, and stars have been measured over the absolute visual magnitude range -1.2 to 8.4. The cluster is shown to contain a significant number of blue straggler stars in the central regions, and there is an excess of objects brighter and redder than the single-star main-sequence in the color-magnitude plane. These objects are interpreted as a population of main-sequence binary stars. With this interpretation, the explicity measured fraction of binary stars is 10 percent, which sets a lower limit for the total binary population.

Bolte, Michael↗

Microbial Extremophiles in Aspect of Limits of Life

During Earth's evolution accompanied by geophysical and climatic changes a number of ecosystems have been formed. These ecosystems differ by the broad variety of physicochemical and biological factors composing our environment. Traditionally, pH and salinity are considered as geochemical extremes, as opposed to the temperature, pressure and radiation that are referred to as physical extremes (Van den Burg, 2003). Life inhabits all possible places on Earth interacting with the environment and within itself (cross species relations). In nature it is very rare when an ecotope is inhabited by a single species. As a rule, most ecosystems contain the functionally related and evolutionarily adjusted communities (consortia and populations). In contrast to the multicellular structure of eukaryotes (tissues, organs, systems of organs, whole organism), the highest organized form of prokaryotic life in nature is the benthic colonization in biofilms and microbial mats. In these complex structures all microbial cells of different species are distributed in space and time according to their functions and to physicochemical gradients that allow more effective system support, self-protection, and energy distribution. In vitro, of course, the most primitive organized structure for bacterial and archaeal cultures is the colony, the size, shape, color, consistency, and other characteristics of which could carry varies specifics on species or subspecies levels. In table 1 all known types of microbial communities are shown (Pikuta et a]., 2005). In deep underground (lithospheric) and deep-sea ecosystems an additional factor - pressure, and irradiation - could also be included in the list of microbial communities. Currently the beststudied ecosystems are: human body (due to the medical importance), and fresh water and marine ecosystems (due to the reason of an environmental safety). For a long time, extremophiles were terra incognita, since the environments with aggressive parameters (compared to the human body temperature, pH, mineralization, and pressure) were considered a priori as a dead zone.

Pikuta, Elena V.↗

Plasma and Brain Cytokine Responses to Single and Combinatorial Spaceflight Stressors in Male and Female Mice

Spaceflight involves exposure to multiple environmental stressors, including Ionizing Radiation (IR), microgravity, and social isolation. We hypothesize that spaceflight stressors combine synergistically to trigger an oxidative stress response that, in turn, alters immune homeostasis, brain structure/function, and neurobehavioral/cognitive performance. Here, we report plasma and brain cytokine findings from two studies of crew age-matched (24-week-old) male and female mice exposed to simulated 5-Ion GCRsim at the NASA Space Radiation Laboratory (NSRL). In Study 1, mice were exposed to 0, 5, 15, or 50cGy 5-ion GCRsim. Immune and brain measures were acquired either at ‘Acute’ (within 3 days post-irradiation; IR), ‘Intermediate’ (IR+14 days) or ‘Delayed’ (IR+125 days) timepoints. In Study 2, mice were exposed singly and in combination to 15cGy 5-ion GCRsim, simulated microgravity via continuous head-down tilt/hindlimb unloading (HU), and social isolation. Plasma and brain cytokine cytokine profiling revealed sex-specific as well as sex-common responses. Notably, plasma granulocyte colony stimulating factor (GCSF) levels showed significant regulation compared to a single stressor alone, suggesting a potential biomarker responder. Increased GCSF was correlated with corresponding increased blood neutrophil populations. Futhermore, our study provides evidence for robust effects of HU relative to IR alone. This project addresses NASA’s efforts to characterize risks in both women and men in anticipation of future space missions beyond low earth orbit (BLEO). Ensuring crew health and performance during extended transits to the moon and beyond necessitates that sensorimotor and cognitive abilities remain strong to avoid potentially catastrophic health and safety outcomes.

mouse radiation hindlimb unloading spaceflight↗

Mechanical Unloading Effects Hematopoiesis and Subsequent Red Blood Cell Life Cycle Evaluated at Singe Cell Resolution

Red blood cell formation or erythropoiesis in the bone marrow and exit into circulation are known to be affected by mechanical loading. Specifically, space anemia is a condition observed during prolonged microgravity unloading in which the red blood cell (RBC) mass in circulation is reduced. However, the mechanisms causing space anemia are yet to be clearly established, but may include increased hemolysis of RBC in circulation, decreased RBC production or a combination of both factors. The presentation of space anemia is comparable to chronic anemia conditions associated with aging, disuse, and poor health. Molecular dysregulation in the RBC's life cycle is poorly defined and is a significant concern in the context of disuse anemia as mechanotransduction significantly alters of gene expression, protein synthesis and cell signaling pathways. Our study analyzes mouse single cell RNA sequencing libraries from the bone marrow, circulating blood, and spleen and integrates the RBC lineage populations from the tissues into a RBC life cycle atlas. This study also probes how age at time of spaceflight contributes to the molecular signature of space anemia. The overarching objective of the study is to develop a mechanistic understanding of space anemia and translate the findings to a better understanding of aging, disuse, and poor health-related anemia to defining the role of mechanical loading in health and devise potential therapeutic interventions. Important findings of our study are: 1) that mechanical unloading in microgravity alters the proportion of developmental RBC lineage cells, skewing toward progenitor, indicating that fewer RBCs may be present in blood; 2) that late reticulocyte number is elevated in the bone marrow of older animals, suggesting a failure to enter circulation; and 3) that the molecular signature of the circulating RBCs is not significantly altered in microgravity. However, expression markers for eryptosis and erythrophagocytosis are elevated in circulating and spleen RBCs, suggesting spaceflight may shorten the lifetime of RBCs after exit from the marrow. In conclusion our results suggest early stages of erythropoiesis proliferation and differentiation in the marrow are key factors in space anemia and mechanical loading may be a necessary step of RBC entry in circulation.

blood↗

Specialization of the DNA-Cleaving Activity of a Group I Ribozyme Through In Vitro Evolution

In an earlier study, an in vitro evolution procedure was applied to a large population of variants of the Tetrahymena group 1 ribozyme to obtain individuals with a 10(exp 5)-fold improved ability to cleave a target single-stranded DNA substrate under simulated physiological conditions. The evolved ribozymes also showed a twofold improvement, compared to the wild-type, in their ability to cleave a single-stranded RNA substrate. Here, we report continuation of the in vitro evolution process using a new selection strategy to achieve both enhanced DNA and diminished RNA-cleavage activity. Our strategy combines a positive selection for DNA cleavage with a negative selection against RNA binding. After 36 "generations" of in vitro evolution, the evolved population showed an approx. 100-fold increase in the ratio of DNA to RNA-cleavage activity. Site-directed mutagenesis experiment confirmed the selective advantage of two covarying mutations within the catalytic core of ribozyme that are largely responsible for this modified behavior. The population of ribozymes has now undergone a total of 63 successive generations of evolution, resulting in an average 28 mutations relative to the wild-type that are responsible for the altered phenotype.

Tsang, Joyce↗

Constraining the Metallicities, Ages, Star Formation Histories, and Ionizing Continua of Extragalactic Massive Star Populations

We infer the properties of massive star populations using the far-ultraviolet stellar continua of 61 star-forming galaxies: 42 at low redshift observed with the Hubble Space Telescope and 19 at z ~ 2 from the MegaSaura sample. We fit each stellar continuum with a linear combination of up to 50 single-age and single-metallicity starburst99 models. From these fits, we derive light-weighted ages and metallicities, which agree with stellar wind and photospheric spectral features, and infer the spectral shapes and strengths of the ionizing continua. Inferred light-weighted stellar metallicities span 0.05–1.5 Z(sub ⊙) and are similar to the measured nebular metallicities. We quantify the ionizing continua using the ratio of the ionizing flux at 900 Å to the non-ionizing flux at 1500 Å and demonstrate the evolution of this ratio with stellar age and metallicity using theoretical single-burst models. These single-burst models only match the inferred ionizing continua of half of the sample, while the other half are described by a mixture of stellar ages. Mixed-age populations produce stronger and harder ionizing spectra than continuous star formation histories, but, contrary to previous studies that assume constant star formation, have similar stellar and nebular metallicities. Stellar population age and metallicity affect the far-UV continua in different and distinguishable ways; assuming a constant star formation history diminishes the diagnostic power. Finally, we provide simple prescriptions to determine the ionizing photon production efficiency (ξ(sub ion)) from the stellar population properties. The ξ(sub ion) inferred from the observed star-forming galaxies has a range of log(ξ(sub ion)) = 24.4–25.7 Hz erg(exp −1) that depends on the stellar population age, metallicity, star formation history, and contributions from binary star evolution. These stellar population properties must be observationally determined to accurately determine the number of ionizing photons generated by massive stars.

Chisholm, J.↗

A Multiphase Model for the Intracluster Medium

Constraints on the clustered mass density of the universe derived from the observed population mean intracluster gas fraction of x-ray clusters may be biased by reliance on a single-phase assumption for the thermodynamic structure of the intracluster medium (ICM). We propose a descriptive model for multiphase structure in which a spherically symmetric ICM contains isobaric density perturbations with a radially dependent variance. Fixing the x-ray emission and emission weighted temperature, we explore two independently observable signatures of the model in the parameter space. For bremsstrahlung dominated emission, the central Sunyaev-Zel'dovich (SZ) decrement in the multiphase case is increased over the single-phase case and multiphase x-ray spectra in the range 0.1-20 keV are flatter in the continuum and exhibit stronger low energy emission lines than their single-phase counterpart. We quantify these effects for a fiducial 10e8 K cluster and demonstrate how the combination of SZ and x-ray spectroscopy can be used to identify a preferred location in the plane of the model parameter space. From these parameters the correct value of mean intracluster gas fraction in the multiphase model results, allowing an unbiased estimate of clustered mass density to he recovered.

Nagai, Daisuke↗

Strategies for Prompt Searches for GRB Afterglows: The Discovery of GRB 001011 Optical/Near-Infrared Counterpart Using Colour-Colour Selection

We report the discovery of the optical and near-infrared counterpart to GRB 001011. The GRB 001011 error box determined by Beppo-SAX was simultaneously imaged in the near-infrared by the 3.58-m. New Technology Telescope and in the optical by the 1.54-m Danish Telescope - 8 hr after the gamma-ray event. We implement the colour-colour discrimination technique proposed by Rhoads (2001) and extend it using near-IR data as well. We present the results provided by an automatic colour-colour discrimination pipe-line developed to discern the different populations of objects present in the GRB 001011 error box. Our software revealed three candidates based on single-epoch images. Second-epoch observations carried out approx. 3.2 days after the burst revealed that the most likely candidate had faded thus identifying it with the counterpart to the GRB. In deep R-band images obtained 7 months after the burst a faint (R=25.38 plus or minus 0.25) elongated object, presumably the host galaxy of GRB 001011, was detected at the position of the afterglow. The GRB 001011 afterglow is the first discovered with the assistance of colour-colour diagram techniques. We discuss the advantages of using this method and its application to boxes determined by future missions.

Gorosabel, J.↗