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At least 145 records · Page 8
All sourcesNASA NTRSDOE OSTI
NASA NTRS1980

Investigation of the effect of space flight factors on chromosomes in seeds

Frequency of mutations in postflight air-dry Crepis capillaris seeds, kept for five days aboard an artificial satellite was 36.0 + or - 1.9%, vs. 19.01 + or - 1.2% for seeds in a similar ground experiment. Both groups of seeds were prepared in identical concentrations and were treated by the same mutagen. The spectrum of mutations in postflight Crepis capillaris seeds contained a large number of chromosome-type mutations and some cells showed multiple alterations. Postflight seeds not treated with mutagens had a slightly higher level of mutability.

Dubinina, L. G.↗
NASA NTRS1983

Seeded crystal growth of pseudobinary systems with large liquidus-solidus separation

The design and execution of seeded melt growth involving concentrated alloys is strongly dependent on the crystal-melt interface temperature and its change during seeding and growth. Thermodynamic instabilities during the seeding process can be controlled by the choice of the seed and melt composition and by the axial temperature gradient of the growth apparatus. These basic factors controlling seeded melt growth of concentrated alloys are of major importance for planning space experiments.

Bourret, E. D.↗
NASA NTRS1985

LDA seeding system for the Langley Low Turbulence Pressure Tunnel

A Laser Velocimetry (LV) seeding system was specifically developed for the Langley Low Turbulence Wind Tunnel (LTPT), and it has been successfully used for LV measurements in two major tests (Juncture Flow Experiment and Gortler Experiment). The LTPT is capable of operating at Mach numbers from 0.05 to 0.50 and unit Reynolds numbers from 100,000 to 15,000,000 per foot. The test section is 3 feet wide and 7.5 feet high. The turbulence level in the test section is relatively low because of the high contraction ratio and because of the nine turbulence reduction screens in the settling chamber. A primary requirement of the seeding system was that the seeding material not contaminate or damage in any way these screens. Both solid and liquid seeding systems were evaluated, and the results are presented. They can provide some guidelines for setting up seeding systems in other similar tunnels.

Scheiman, J.↗
NASA NTRS1992

Final results of the Space Exposed Experiment Developed for Students (SEEDS) P-0004-2

Space Exposed Experiment Developed for Students (SEEDS), resulted in the distribution of over 132,000 SEED kits in 1990. The kits contained Rutger's tomato seeds that had flown on the Long Duration Exposure Facility (LDEF) as well as seeds that had been stored in a climate controlled warehouse for the same period of time. Students compared germination and growth rate characteristics of the two seeds groups and returned data to NASA for analysis. The scientific information gained was valuable as students shared the excitement of taking part in a national project. Of greater importance was the subsequent interest generated in science education.

Grigsby, Doris K.↗
NASA NTRS1994

Mutational effects of space flight on Zea mays seeds

The growth and development of more than 500 Zea mays seeds flown on Long Duration Exposure Facility (LDEF) were studied. Somatic mutations, including white-yellow stripes on leaves, dwarfing, change of leaf sheath color or seedling color were observed in plants developed from these seeds. When the frequency of white-yellow formation was used as the endpoint and compared with data from ground based studies, the dose to which maize seeds might be exposed during the flight was estimated to be equivalent to 635 cGy of gamma rays. Seeds from one particular holder gave a high mutation frequency and a wide mutation spectrum. White-yellow stripes on leaves were also found in some of the inbred progenies from plants displayed somatic mutation. Electron microscopy studies showed that the damage of chloroplast development in the white-yellow stripe on leaves was similar between seeds flown on LDEF and that irradiated by accelerated heavy ions on ground.

Mei, M.↗
NASA NTRS1995

Demonstration of a stabilized alumina/ethanol colloidal dispersion technique for seeding high temperature air flows

Laser anemometry enables the measurement of complex flow fields via the light scattered from small particles entrained in the flow. In the study of turbomachinery, refractory seed materials are required for seeding the flow due to the high temperatures encountered. In this work we present a pH stabilization technique commonly employed in ceramic processing to obtain stable dispersions for generating aerosols of refractory seed material. By adding submicron alumina particles to a preadjusted pH solution of ethanol, a stable dispersion is obtained which when atomized, produces a high quality aerosol. Commercial grade alumina powder is used with a moderate size distribution. Other metal oxide powders in various polar solvents could also be used once the point of zero charge (pH(pzc)) of the powder in the solvent has been determined. Laser anemometry measurements obtained using the new seeding technique are compared to measurements obtained using Polystyrene Latex (PSL) spheres as the seed material.

Wernet, Mark P.↗
NASA NTRS1988

Effect of salicylhydroxamic acid on endosperm strength and embryo growth of Lactuca sativa L. cv Waldmann's Green seeds

Salicylhydroxamic acid (SHAM) stimulated germination of photosensitive lettuce (Lactuca sativa L. cv Waldmann's Green) seeds in darkness. To determine whether SHAM acts on the embryo or the endosperm, we investigated separately effects of SHAM on growth potential of isolated embryos as well as on endosperm strength. Embryo growth potential was quantified by incubating decoated embryos in various concentrations of osmoticum and measuring subsequent radicle elongation. Growth potential of embryos isolated from seeds pretreated with 4 millimolar SHAM was equal to that of untreated controls. Rupture strength of endosperm tissue excised from seeds pretreated with SHAM was 33% less than that of controls in the micropylar region. To determine if the embryo must be in contact with the endosperm of SHAM to weaken the endosperm, some endosperms were incubated with SHAM only after dissection from seeds. Rupture strength of SHAM-treated, isolated endosperms in the micropylar region was 25% less than that of untreated controls. There was no difference in rupture strength in the cotyledonary region of endosperm isolated from seeds treated with SHAM in buffer or buffer alone. SHAM therefore stimulates germination not by enhancing embryo growth potential, but by weakening the micropylar region of the endosperm enclosing the embryo.

NASA Discipline Plant Biology↗
NASA NTRS2010

Fiber-Coupled Planar Light-Wave Circuit for Seed Laser Control in High Spectral Resolution Lidar Systems

Precise laser remote sensing of aerosol extinction and backscatter in the atmosphere requires a high-power, pulsed, frequency doubled Nd:YAG laser that is wavelength- stabilized to a narrow absorption line such as found in iodine vapor. One method for precise wavelength control is to injection seed the Nd:YAG laser with a low-power CW laser that is stabilized by frequency converting a fraction of the beam to 532 nm, and to actively frequency-lock it to an iodine vapor absorption line. While the feasibility of this approach has been demonstrated using bulk optics in NASA Langley s Airborne High Spectral Resolution Lidar (HSRL) program, an ideal, lower cost solution is to develop an all-waveguide, frequency-locked seed laser in a compact, robust package that will withstand the temperature, shock, and vibration levels associated with airborne and space-based remote sensing platforms. A key technology leading to this miniaturization is the integration of an efficient waveguide frequency doubling element, and a low-voltage phase modulation element into a single, monolithic, planar light-wave circuit (PLC). The PLC concept advances NASA's future lidar systems due to its compact, efficient and reliable design, thus enabling use on small aircraft and satellites. The immediate application for this technology is targeted for NASA Langley's HSRL system for aerosol and cloud characterization. This Phase I effort proposes the development of a potassium titanyl phosphate (KTP) waveguide phase modulator for future integration into a PLC. For this innovation, the proposed device is the integration of a waveguide-based frequency doubler and phase modulator in a single, fiber pigtail device that will be capable of efficient second harmonic generation of 1,064-nm light and subsequent phase modulation of the 532 nm light at 250 MHz, providing a properly spectrally formatted beam for HSRL s seed laser locking system. Fabrication of the integrated PLC chip for NASA Langley, planned for the Phase II effort, will require full integration and optimization of the waveguide components (SHG waveguide, splitters, and phase modulator) onto a single, monolithic device. The PLC will greatly reduce the size and weight, improve electrical- to-optical efficiency, and significantly reduce the cost of NASA Langley s current stabilized HSRL seed laser system built around a commercial off-the-shelf seed laser that is free-space coupled to a bulk doubler and bulk phase modulator.

Cook, Anthony↗
NASA NTRS2013

Cirrus Cloud Seeding has Potential to Cool Climate

Cirrus clouds, thin ice clouds in the upper troposphere, have a net warming effect on Earth s climate. Consequently, a reduction in cirrus cloud amount or optical thickness would cool the climate. Recent research indicates that by seeding cirrus clouds with particles that promote ice nucleation, their lifetimes and coverage could be reduced. We have tested this hypothesis in a global climate model with a state-of-the-art representation of cirrus clouds and find that cirrus cloud seeding has the potential to cancel the entire warming caused by human activity from pre-industrial times to present day. However, the desired effect is only obtained for seeding particle concentrations that lie within an optimal range. With lower than optimal particle concentrations, a seeding exercise would have no effect. Moreover, a higher than optimal concentration results in an over-seeding that could have the deleterious effect of prolonging cirrus lifetime and contributing to global warming.

Storelvmo, T.↗
NASA NTRS2014

Structural Characterization of Lateral-grown 6H-SiC am-plane Seed Crystals by Hot Wall CVD Epitaxy

The performance of commercially available silicon carbide (SiC) power devices is limited due to inherently high density of screw dislocations (SD), which are necessary for maintaining polytype during boule growth and commercially viable growth rates. The NASA Glenn Research Center (GRC) has recently proposed a new bulk growth process based on axial fiber growth (parallel to the c-axis) followed by lateral expansion (perpendicular to the c-axis) for producing multi-faceted m-plane SiC boules that can potentially produce wafers with as few as one SD per wafer. In order to implement this novel growth technique, the lateral homoepitaxial growth expansion of a SiC fiber without introducing a significant number of additional defects is critical. Lateral expansion is being investigated by hot wall chemical vapor deposition (HWCVD) growth of 6H-SiC am-plane seed crystals (0.8mm x 0.5mm x 15mm) designed to replicate axially grown SiC single crystal fibers. The post-growth crystals exhibit hexagonal morphology with approximately 1500 m (1.5 mm) of total lateral expansion. Preliminary analysis by synchrotron white beam x-ray topography (SWBXT) confirms that the growth was homoepitaxial, matching the polytype of the respective underlying region of the seed crystal. Axial and transverse sections from the as grown crystal samples were characterized in detail by a combination of SWBXT, transmission electron microscopy (TEM) and Raman spectroscopy to map defect types and distribution. X-ray diffraction analysis indicates the seed crystal contained stacking disorders and this appears to have been reproduced in the lateral growth sections. Analysis of the relative intensity for folded transverse acoustic (FTA) and optical (FTO) modes on the Raman spectra indicate the existence of stacking faults. Further, the density of stacking faults is higher in the seed than in the grown crystal. Bundles of dislocations are observed propagating from the seed in m-axis lateral directions. Contrast extinction analysis of these dislocation lines reveals they are edge type basal plane dislocations that track the growth direction. Polytype phase transition and stacking faults were observed by high-resolution TEM (HRTEM), in agreement with SWBXT and Raman scattering.

Single Crystal↗
NASA NTRS2015

Investigation of Gas Seeding for Planar Laser-Induced Fluorescence in Hypersonic Boundary Layers

Numerical simulations of the gas-seeding strategies required for planar laser-induced fluorescence in a Mach 10 (approximately Mach 8.2 postshock) airflow were performed. The work was performed to understand and quantify the adverse effects associated with gas seeding and to assess various types of seed gas that could potentially be used in future experiments. In prior experiments, NO and NO2 were injected through a slot near the leading edge of a flatplate wedge model used in NASA Langley Research Center's 31 in. Mach 10 air tunnel facility. In this paper, nitric oxide, krypton, and iodine gases were simulated at various injection rates. Simulations showing the deflection of the velocity boundary layer for each of the cases are presented. Streamwise distributions of velocity and concentration boundary-layer thicknesses, as well as vertical distributions of velocity, temperature, and mass distributions, are presented for each of the cases. A comparison between simulated streamwise velocity profiles and experimentally obtained molecular tagging velocimetry profiles using a nitric oxide seeding strategy is performed to verify the influence of such a strategy on the boundary layer. The relative merits of the different seeding strategies are discussed. The results from a custom solver based on OpenFOAM version 2.2.1 are compared against results obtained from ANSYS® Fluent version 6.3.

Arisman, C. J.↗
NASA NTRSDate not supplied

Changes in Plants Developed from Imbibed Seeds Irradiated by Simulated Solar Particle Events

One of the major concerns for long-term exploration missions beyond the Earth’s magnetosphere is radiation risk primarily from solar particle events (SPEs) and galactic cosmic rays (GCRs). With the goal of enabling manned Mars exploration, the production of fresh food during long duration space missions may provide nutritional supplementation and benefit astronauts’ behavioral health. However, the effects of space radiation on plants and plant propagules have not been sufficiently investigated and characterized. In this study, we evaluated the effect of simulated SPEs on imbibed seeds of mizuna mustard, ‘Outredgeous’ red romaine lettuce, and ‘Red Robin’ dwarf tomato. Seeds were exposed to various doses of simulated SPEs (0, 40, 80 cGy, and 200 cGy), either acutely or at a low dose rate (LDR only for 40 and 80 cGy doses), using the NASA Space Radiation Laboratory (NSRL) facility at Brookhaven National Lab (BNL). Simulated SPE exposure was achieved using sequential exposures to protons with a broad energy range at 50-150 MeV/n. Control and irradiated seeds were planted either onto 0.5% MS-based agar plates containing required nutrients for early seedling analysis, or in peat plugs/pots for 28 day (mizuna and lettuce) and 91 day (tomato) grow outs. All plants were maintained under space-vehicle relevant conditions at 3000 ppm CO2, 50% RH, and 23°C, and a 16/8 h photoperiod with 300 μmol·m(exp -2)·s(exp -1) photosynthetically active radiation. Plant/fruit morphometrics and edible fresh mass were measured. Additionally, nutrient analyses were performed on mizuna and tomato fruit. Seedlings from irradiated seeds (both 40 and 80 cGy) showed significant and dose dependent impacts on cotyledon development and root length. Interestingly, small, but significant changes in vitamin content were found in mizuna and tomato fruits grown from 80 cGy (LDR) irradiated seeds. In addition, 80 cGy (LDR) simulated SPEs significantly delayed tomato early seedling development and plant growth, and reduced the quantity of fruits produced from the surviving plants. (This study was supported by NASA Human Research Program.)

J. T. Richards↗
NASA NTRSDate not supplied

Particle Seeding Method for Small-Scale, High-Pressure Nozzles

NASA tests new launch and reentry vehicle configurations in wind tunnels, where flow visualizations and quantitative flowfield measurements are often desired. Some of these vehicles have rocket motors for propulsion, retro-propulsion, or reaction control. High-pressure air is used to supply these rocket motor plumes. However, it is difficult to make off-body measurements in these regions, for several reasons. First, the plumes themselves lack seeding particles for flow diagnostics, and at the low pressures after expansion, Rayleigh scattering or other molecular techniques yield insufficient signal for flow velocity measurements. Second, the plumes displace particle-seeded tunnel air, preventing measurements in the vicinity of the plume. Third, the plumes force shock waves ahead of the vehicle, which melts the ice crystal fog commonly used for visualization and measurement techniques in certain facilities. In the current work, a novel method for seeding the flow in these small-scale, high-pressure nozzles has been devised and initially demonstrated, potentially enabling quantitative and qualitative measurements with particle-based instruments such as Doppler global velocimetry or particle image velocimetry. The method involves a Venturi contraction to draw the seed liquid out of a reservoir and into the nozzle channel, wherein shearing forces atomize the seed into particles. The concept was tested with a laser sheet visualization, which demonstrated that the flow rate of liquid spray was controllable; a valve could be adjusted to drop the flow rate by up to 65%. This relatively inexpensive and simple technique may prove useful in wind tunnel experiments involving particle-based laser diagnostics and small, high-pressure nozzles.

Aditya S. Acharya↗
NASA NTRSDate not supplied

Particle Seeding Method for Small-Scale, High-Pressure Nozzles

NASA tests new launch and reentry vehicle configurations in wind tunnels, where flow visualizations and quantitative flowfield measurements are often desired. Some of these vehicles have rocket motors for propulsion, retro-propulsion, or reaction control. High-pressure air is used to supply these rocket motor plumes. However, it is difficult to make off-body measurements in these regions, for several reasons. First, the plumes themselves lack seeding particles for flow diagnostics, and at the low pressures after expansion, Rayleigh scattering or other molecular techniques yield insufficient signal for flow velocity measurements. Second, the plumes displace particle-seeded tunnel air, preventing measurements in the vicinity of the plume. Third, the plumes force shock waves ahead of the vehicle, which melts the ice crystal fog commonly used for visualization and measurement techniques in certain facilities. In the current work, a novel method for seeding the flow in these small-scale, high-pressure nozzles has been devised and initially demonstrated, potentially enabling quantitative and qualitative measurements with particle-based instruments such as Doppler global velocimetry or particle image velocimetry. The method involves a Venturi contraction to draw the seed liquid out of a reservoir and into the nozzle channel, wherein shearing forces atomize the seed into particles. The concept was tested with a laser sheet visualization, which demonstrated that the flow rate of liquid spray was controllable; a valve could be adjusted to drop the flow rate by up to 65%. This relatively inexpensive and simple technique may prove useful in wind tunnel experiments involving particle-based laser diagnostics and small, high-pressure nozzles.

lasers↗
NASA NTRSDate not supplied

Transcriptomic Changes in Seedlings from Seeds Exposed to Simulated Space Radiation

Outside the protection of Earth’s magnetic field, living organisms are constantly exposed to space radiation that consists of energetic protons and other heavier charged particles. With the goal of manned Mars exploration, the production of fresh crop during long duration space missions can be beneficial for meeting astronauts’ nutritional and psychological needs. In our study, we not only evaluated plant/fruit morphometrics and edible fresh mass, but also analyzed transcriptomic changes in seedlings from seeds of three plant species (Arabidopsis, mizuna, and tomato) exposed to simulated Galactic Cosmic Rays(GCR) and solar particle events(SPE). The radiation experiments were performed in the NASA Space Radiation Laboratory (NSRL) facility at Brookhaven National Lab (BNL). 10-day Arabidopsis seedlings were exposed acutely (~240 cGy/hr) to simulated GCR scenarios of combined ions including protons, helium, oxygen, titanium, and/or iron ions at 40 or 80 cGy. Seeds of Arabidopsis, mizuna, and tomato were exposed to 40 or 80 cGy simulated GCR (dry seeds) or SPE (imbibed seeds) at lower dose rates(20-26 cGy/hr). Seedlings from control and irradiated seeds were then collected in RNAlater at similar growth stages with true leaves emerged. Total RNA was isolated and analyzed via Illumina whole transcriptome sequencing technology. Plant species-specific bioinformatics revealed transcriptional biomarkers and signaling pathways induced by simulated space radiation that were found to be dose, dose-rate, and species dependent. DNA damage response, stress signaling, and metabolic pathways are among the most significant changes. These data highlight some critical insights on the mechanisms of how plants respond and adapt to the space radiation environment and provide a molecular basis for crop selection and refinement in deep space exploration.

Anirudha Dixit↗
DOE OSTI2021

A 3D FPGA Track Segment Seeding Engine Based on the Tiny Triplet Finder

A 3D track segment seeding engine core based on the Tiny Triplet Finder has been implemented and tested in a low-cost FPGA device. The seeding engine is designed to preselect and group hits (stubs) from cylindrical detector layers to feed subsequent track fitting stage. The seeding engine consists of a Hough transform space for r-z view and a Tiny Triplet Finder for r-phi view to implement 3D constraints. The seeding engine is organized as a pipeline so that each hit is processed in a single clock cycle. Test results show that the seeding engine operates as expected.

Wu, Jinyuan↗
DOE OSTI2024

Seed classification with random forest models

Premise: To improve forest conservation monitoring, we developed a protocol to automatically count and identify the seeds of plant species with minimal resource requirements, making the process more efficient and less dependent on human operators. Methods and Results: Seeds from six North American conifer tree species were separated from leaf litter and imaged on a flatbed scanner. In the most successful species-classification approach, an ImageJ macro automatically extracted measurements for random forest classification in the software R. The method allows for good classification accuracy, and the same process can be used to train the model on other species. Conclusions: This protocol is an adaptable tool for efficient and consistent identification of seed species or potentially other objects. Automated seed classification is efficient and inexpensive, making it a practical solution that enhances the feasibility of large-scale monitoring projects in conservation biology.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2026

Why Seeding Works When Nucleation Barriers Vanish

This paper explains why seeds can speed up crystallization even when the formation of new crystal nuclei is not the slow step. In zeolite synthesis, local crystalline order can form relatively early, but the material may still appear poorly crystalline by X-ray diffraction because many small crystallites are misoriented and lack long-range coherence. Using coarse-grained molecular simulations, the study showed that seeds help solve this coherence problem. Rather than primarily lowering a nucleation barrier, seeds impose a common orientation on nearby growing crystallites, allowing them to merge into larger, better aligned domains. This accelerates the development of X-ray-detectable crystallinity even if the total amount of locally ordered material has not changed much. The work changes how induction times in zeolite synthesis should be interpreted: the delay before X-ray crystallinity appears can reflect the time needed to build long-range structural coherence, not simply the time needed to form crystalline material. This provides a new explanation for why seeding is effective in growth-limited crystallization and offers design principles for controlling crystallization rate and crystal quality.

36 MATERIALS SCIENCE↗