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At least 145 records · Page 8

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DOE OSTI2022

Size and Distribution of Parr Produced from Natural‐ and Hatchery‐Origin Steelhead Spawning Naturally in a Small Pacific Northwest Coastal Stream

Abstract Recent studies suggest that steelhead Oncorhynchus mykiss produced from local hatchery‐origin (HOR) adults have lower lifetime fitness than their natural‐origin (NOR) counterparts. To increase our understanding of this pattern, we compared age‐1 parr size and distribution produced by a local integrated population of HOR and NOR adults spawning naturally across a range of environmental conditions. Across 8 years, we used genetic parentage assignments and field measurements of parr in conjunction with creek temperature and flow data to find small, nonbiologically significant differences in size between parr of different parent origins. This suggests that parr produced by HOR adults are acquiring enough resources to grow at a rate similar to that of parr produced by NOR adults. In contrast to origin, we found strong positive associations between mean size of parr and annual mean water temperature and summer flow. Additionally, the distribution of parr was similar and HOR‐produced parr were not skewed relative to the location of the hatchery. Parr occupying the full extent of accessible and suitable habitat might be a positive outcome for hatchery programs seeking to supplement an existing population or replace an extirpated population. However, these results also imply that HOR fish could be competing with NOR fish for food and space, which might be less desirable if the goal of the hatchery program is harvest and not supplementation. Our results highlight the importance of clearly articulated goals for the hatchery program, as the observed pattern could be deemed a benefit or a risk depending on the hatchery's purpose. Lastly, projections for higher water temperatures and reduced summer flows, when considered in the context of the correlations we observed for size of parr, imply that coastal steelhead populations could start experiencing negative climate impacts with respect to steelhead parr growth metrics during the summer in the next few decades.

Kennedy, Benjamen M.↗

DOE OSTI2018

Isolation and characterization of novel mutations in the pSC101 origin that increase copy number

pSC101 is a narrow host range, low-copy plasmid commonly used for genetically manipulating Escherichia coli. As a byproduct of a genetic screen for a more sensitive lactam biosensor, we identified multiple novel mutations that increase the copy number of plasmids with the pSC101 origin. All mutations identified in this study occurred on plasmids which also contained at least one mutation localized to the RepA protein encoded within the origin. Homology modelling predicts that many of these mutations occur within the dimerization interface of RepA. Mutant RepA resulted in plasmid copy numbers between ~31 and ~113 copies/cell, relative to ~5 copies/cell in wild-type pSC101 plasmids. Combining the mutations that were predicted to disrupt multiple contacts on the dimerization interface resulted in copy numbers of ~500 copies/cell, while also attenuating growth in host strains. Fluorescent protein production expressed from an arabinose-inducible promoter on mutant origin derived plasmids did correlate with copy number. Plasmids harboring RepA with one of two mutations, E83K and N99D, resulted in fluorescent protein production similar to that from p15a- (~20 copies/cell) and ColE1- (~31 copies/cell) based plasmids, respectively. The mutant copy number variants retained compatibility with p15a, pBBR, and ColE1 origins of replication. Thus, these pSC101 variants may be useful in future metabolic engineering efforts that require medium or high-copy vectors compatible with p15a- and ColE1-based plasmids.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2017

Origin of the extremely large magnetoresistance in the semimetal YSb

Extremely large magnetoresistance (XMR) was recently discovered in YSb but its origin, along with that of many other XMR materials, is an active subject of debate. Here we demonstrate that YSb, with a cubic crystalline lattice and anisotropic bulk electron Fermi pockets, can be an excellent candidate for revealing the origin of XMR. We carried out angle dependent Shubnikov – de Haas quantum oscillation measurements to determine the volume and shape of the Fermi pockets. In addition, by investigating both Hall and longitudinal magnetoresistivities, we reveal that the origin of XMR in YSb lies in its carrier high mobility with a diminishing Hall factor that is obtained from the ratio of the Hall and longitudinal magentoresistivities. The high mobility leads to a strong magnetic field dependence of the longitudinal magnetoconductivity while a diminishing Hall factor reveals the latent XMR hidden in the longitudinal magnetoconductivity whose inverse has a nearly quadratic magnetic-field dependence. The Hall factor highlights the deviation of the measured magnetoresistivity from its full potential value and provides a general formulation to reveal the origin of XMR behavior in high mobility materials and of nonsaturating MR behavior as a whole. Our approach can be readily applied to other XMR materials.

36 MATERIALS SCIENCE↗

DOE OSTI2022

Finding evidence for inflation and the origin of galactic magnetic fields with CMB surveys

The origin of the μG magnetic fields observed in galaxies is unknown. One promising scenario is that magnetic fields generated during inflation, larger than 0.1 nG on Mpc scales, were adiabatically compressed to μG strengths in galaxies during structure formation. Thus, detecting a scale-invariant primordial magnetic field (PMF) above 0.1 nG on Mpc scales just after recombination would indicate an inflationary origin of galactic magnetic fields. Furthermore, this would also provide compelling evidence that inflation occurred since only an inflationary mechanism could generate such a strong, scale-invariant magnetic field on Mpc scales. In contrast, constraining the scale-invariant PMF strength to be below 0.1 nG would imply an inflationary scenario is not the primary origin, since such weak PMFs cannot be amplified enough via adiabatic compression to produce the strength of the galactic fields we observe today. We find that measurements of anisotropic birefringence by future CMB surveys will be able to improve the sensitivity to Mpc-scale inflationary PMFs by an order of magnitude, and, in particular, that CMB-HD would lower the upper bound to 0.072 nG at the 95% C.L., which is below the critical 0.1 nG threshold for ruling out a purely inflationary origin. If inflationary PMFs exist, we find that a CMB-HD survey would be able to detect them with about 3σ significance or higher, providing evidence for inflation itself.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

DOE OSTI2022

Impact of salinity origin on microbial communities in saline springs within the Illinois Basin, USA

Abstract Saline springs within the Illinois Basin result from the discharge of deep‐seated evaporated seawater (brine) and likely contain diverse and complex microbial communities that are poorly understood. In this study, seven saline/mineral springs with different geochemical characteristics and salinity origins were investigated using geochemical and molecular microbiological analyses to reveal the composition of microbial communities inhabiting springs and their key controlling factors. The 16S rRNA sequencing results demonstrated that each spring harbours a unique microbial community influenced by its geochemical properties and subsurface conditions. The microbial communities in springs that originated from Cambrian/Ordovician strata, which are deep confined units that have limited recharge from overlying formations, share a greater similarity in community composition and have a higher species richness and more overlapped taxa than those that originated from shallower Pennsylvanian strata, which are subject to extensive regional surface and groundwater recharge. The microbial distribution along the spring flow paths at the surface indicates that 59.8%–94.2% of total sequences in sedimentary samples originated from spring water, highlighting the role of springs in influencing microbiota in the immediate terrestrial environment. The results indicate that the springs introduce microbiota with a high biodiversity into surface terrestrial or aquatic ecosystems, potentially affecting microbial reservoirs in downstream ecosystems.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2017

Evidence of an Internal Dissipation Origin for the High-energy Prompt Emission of GRB 170214A

The origin of the prompt high-energy (>100 MeV) emission of gamma-ray bursts (GRBs), detected by the Large Area Telescope (LAT) on board the Fermi Gamma-ray Space Telescope, for which both an external shock origin and internal dissipation origin have been suggested, is still under debate. In the internal dissipation scenario, the high-energy emission is expected to exhibit significant temporal variability, tracking the keV/MeV fast variable behavior. Here, we report a detailed analysis of the Fermi data of GRB 170214A, which is sufficiently bright in high energies to enable a quantitative analysis of the correlation between high-energy emission and keV/MeV emission with high statistics. Our result shows a clear temporal correlation between high-energy and keV/MeV emission in the whole prompt emission phase as well as in two decomposed short time intervals. Such a correlation is also found in some other bright LAT GRBs, e.g., GRB 080916C, 090902B, and 090926A. For these GRBs as well as GRB 090510, we also find rapid temporal variability in the high-energy emission. We thus conclude that the prompt high-energy emission in these bright LAT GRBs should be due to an internal origin.

79 ASTRONOMY AND ASTROPHYSICS↗

DOE OSTI2017

The First Ionization Potential Effect from the Ponderomotive Force: On the Polarization and Coronal Origin of Alfvén Waves

We investigate in more detail the origin of chromospheric Alfvén waves that give rise to the separation of ions and neutrals—the first ionization potential (FIP) effect—through the action of the ponderomotive force. In open field regions, we model the dependence of fractionation on the plasma upflow velocity through the chromosphere for both shear (or planar) and torsional Alfvén waves of photospheric origin. These differ mainly in their parametric coupling to slow mode waves. Shear Alfvén waves appear to reproduce observed fractionations for a wider range of model parameters and present less of a “fine-tuning” problem than do torsional waves. In closed field regions, we study the fractionations produced by Alfvén waves with photospheric and coronal origins. Waves with a coronal origin, at or close to resonance with the coronal loop, offer a significantly better match to observed abundances than do photospheric waves, with shear and torsional waves in such a case giving essentially indistinguishable fractionations. Such coronal waves are likely the result of a nanoflare coronal heating mechanism that, as well as heating coronal plasmas, releases Alfvén waves that can travel down to loop footpoints and cause FIP fractionation through the ponderomotive force as they reflect from the chromosphere back into the corona.

79 ASTRONOMY AND ASTROPHYSICS↗

DOE OSTI2017

The Origins of [C ii] Emission in Local Star-forming Galaxies

The [C ii] 158 μ m fine-structure line is the brightest emission line observed in local star-forming galaxies. As a major coolant of the gas-phase interstellar medium, [C ii] balances the heating, including that due to far-ultraviolet photons, which heat the gas via the photoelectric effect. However, the origin of [C ii] emission remains unclear because C{sup +} can be found in multiple phases of the interstellar medium. Here we measure the fractions of [C ii] emission originating in the ionized and neutral gas phases of a sample of nearby galaxies. We use the [N ii] 205 μ m fine-structure line to trace the ionized medium, thereby eliminating the strong density dependence that exists in the ratio of [C ii]/[N ii] 122 μ m. Using the FIR [C ii] and [N ii] emission detected by the KINGFISH (Key Insights on Nearby Galaxies: a Far- Infrared Survey with Herschel ) and Beyond the Peak Herschel programs, we show that 60%–80% of [C ii] emission originates from neutral gas. We find that the fraction of [C ii] originating in the neutral medium has a weak dependence on dust temperature and the surface density of star formation, and has a stronger dependence on the gas-phase metallicity. In metal-rich environments, the relatively cooler ionized gas makes substantially larger contributions to total [C ii] emission than at low abundance, contrary to prior expectations. Approximate calibrations of this metallicity trend are provided.

79 ASTRONOMY AND ASTROPHYSICS↗

NASA NTRS1970

Origins of life; Proceedings of the First Conference, Princeton, N.J., May 21-24, 1967.

The dialogs on the origin of life commence with the fossil record. They deal with the age of the earth; the primitive atmosphere; precambrian microfossils; the Fig Tree series of rocks, which is part of the Swaziland system; the Bitter Springs Formation in Australia; and simulated Precambrian microfossils. The syntheses of biologically important monomers, amino acids, and nucleic acid derivatives are discussed. Polymerization mechanisms; the thermal origin of amino acid polymers; nonnucleic acid information carriers; the relationship between amino acid and nucleic acid polymers; the origin of the triple code; early reciprocating systems; and the evolution of the ribosomal system receive attention. Some brief comments on extraterrestrial life are presented. An appendix deals with the geological evidence for the thermal origin of living systems.

Margulis, L.↗

NASA NTRS1972

Lunar crater origin in the maria from analysis of Orbiter photographs.

Attempt to diagnose the origin of craters that are predominantly between 100 and 2000 m diam by the use of a statistical method that is capable of measuring both the chaining and clustering of craters. It is argued that craters with aligned centers that trend in parallel directions over vast distances are exclusively of internal origin. Strong evidence that a high proportion of the chains are of tectonic origin is found. Other criteria, such as a systematic change in the number density of craters over a test area or an excess of craters on a recent lava flow, are also used to argue for endogenic craters. Using all the data together, it is found that at least 33%, but not more than 51%, of the craters sampled are of endogenic origin. It is shown that the flux of meteoroids has not necessarily varied for the past few billion years.

Fielder, G.↗

NASA NTRS1972

Concepts related to the origin of the genetic apparatus.

Since the genetic apparatus is composed of nucleic acid and protein, the origin of the apparatus involves the origin of polyaminoacids and of polynucleotides, separately or together. Laboratory models for the origins of the separate macromolecular types are considered. The origin of their joint synthesis, believed to have been necessary at a later stage, is also discussed. Proteins and nucleic acids appear to have definite relationships with each other in three major contemporary biological systems, including chromatin, ribosomes, and the protein synthesizing system using nucleic acid templates. Stages considered in connection with the flow of information in the early evolution of the apparatus are environment, protoprotein, protein, RNA, and DNA.

Lacey, J. C., Jr.↗

NASA NTRS1974

Geochemistry and the origin of life

The origin of life on earth is examined from a viewpoint stressing the validity of the concept of chemical evolution. The different geological formations supporting the mechanisms of the theory are described; the stage of chemical evolution (preceding that of biological evolution) would have taken place from the time of the origin of the earth and meteorites, 4.6 billion years ago, to the early Precambrian period, about 3.2 billion years ago. Specific aspects of the problem discussed include amino acids from spark discharges and their comparison with the Murchison meteorite amino acids, the properties and theory of genesis of the carbonaceous complex within the cold Bokevelt meteorite, ammonion ion concentration in the primitive ocean, the oxygen isotope chemistry of ancient charts, the origin and rise of oxygen concentration in the earth's atmosphere, Precambrian microorganisms and evolutionary events prior to the origin of vascular plants, and biogenicity and significance of the oldest known stromatolites.

Kvenvolden, K. A.↗

NASA NTRS1975

Comets and planets - Their interrelated origin

This report gives a concise summary of the conclusions of cosmogonic studies linking the origin of comets with the origin of the Jovian planets at the dawn of the solar system. Cometary nuclei are shown to be original planetesimals ejected from and returning to the solar system. Formation processes for comets are described in detail, emphasizing the preplanetary rings of the Jovian planets, condensation and accretion from diffuse matter, and the origin of Oort's sphere. Physical and mechanical properties of comets are discussed, including the composition of cometary gases, the size and composition of cometary nuclei, the placement of comets in Oort's sphere, and the density of nuclei in that region. Unresolved difficulties in models of planet and comet formation are considered.

Opik, E. J.↗

NASA NTRS1983

Impact of solar system exploration on theories of chemical evolution and the origin of life

The impact of solar system exploration on theories regarding chemical evolution and the origin of life is examined in detail. Major findings from missions to Mercury, Venus, the moon, Mars, Jupiter, Saturn, and Titan are reviewed and implications for prebiotic chemistry are discussed. Among the major conclusions are: prebiotic chemistry is widespread throughout the solar system and universe; chemical evolution and the origin of life are intimately associated with the origin and evolution of the solar system; the rate, direction, and extent of prebiotic chemistry is highly dependent upon planetary characteristics; and continued exploration will increase understanding of how life originated on earth and allow better estimates of the likelihood of similar processes occurring elsewhere.

Devincenzi, D. L.↗

NASA NTRS1983

Multiple origins of life

There is some indication that life may have originated readily under primitive earth conditions. If there were multiple origins of life, the result could have been a polyphyletic biota today. Using simple stochastic models for diversification and extinction, we conclude: (1) the probability of survival of life is low unless there are multiple origins, and (2) given survival of life and given as many as 10 independent origins of life, the odds are that all but one would have gone extinct, yielding the monophyletic biota we have now. The fact of the survival of our particular form of life does not imply that it was unique or superior.

Raup, D. M.↗

NASA NTRS1990

Numerical investigation of the origin of vortex asymmetry

The flow about a slender body of revolution placed at incidence to an oncoming stream is numerically investigated for angles of attack ranging from 20 to 80 degrees and a Reynolds number of 200,000, based on freestream conditions and maximum body diameter. Navier-Stokes computations are applied to investigate the phenomena governing the onset of vortex asymmetry. Time-accurate solutions are given for an ogive-cylinder body with and without a space-fixed time-invariant disturbance added near the tip. At a 40-degree angle of attack the flow is found to be steady but becomes highly asymmetric when the disturbance is added. The level of asymmetry is dependent upon the size and location of the disturbance. It is suggested that the origin of the asymmetry is a convective-type instability of the originally symmetric flow. For higher angles of attack (60 and 80 degrees) the flow around the cylindrical part becomes unsteady, and vortex shedding is observed. It is suggested that the origin of flow unsteadiness and vortex shedding in the wake is an absolute-type instability of the originally steady flow.

Degani, David↗

NASA NTRS1990

The photochemical origins of life and photoreaction of ferrous ion in the archaean oceans

A general argument is made for the photochemical origins of life. A constant flux of free energy is required to maintain the organized state of matter called life. Solar photons are the unique source of the large amounts of energy probably required to initiate this organization and certainly required for the evolution of life to occur. The completion of this argument will require the experimental determination of suitable photochemical reactions. It is shown that biogenetic porphyrins readily photooxidize substrates and emit hydrogen in the presence of a catalyst. These results are consistent with the Granick hypothesis, which relates a biosynthetic pathway to its evolutionary origin. It has been shown that photoexcitation of ferrous ion at neutral pH with near ultraviolet light produces hydrogen with high quantum yield. This same simple system may reduce carbon dioxide to formaldehyde and further products. These reactions offer a solution to the dilemma confronting the Oparin-Urey-Miller model of the chemical origin of life. If carbon dioxide is the main form of carbon on the primitive earth, the ferrous photoreaction may provide the reduced carbon necessary for the formation of amino acids and other biogenic molecules. These results suggest that this progenitor of modern photosynthesis may have contributed to the chemical origins of life.

Mauzerall, David C.↗

NASA NTRS1991

Origin of the Uranian satellites

The current understanding of the origin of the Uranian satellites is assessed by reviewing relevant data on the Uranian satellites, including those obtained by Voyager, and comparing these properties with those of the satellites of the other outer planets. The nature of the early solar system, including the origin of the giant planets, is discussed as a preface to alternative hypotheses for the origin of the nebular disk within which the Uranian satellites formed. The chemical and physical properties of this disk are discussed, as well as the accretion of the satellites from disk solid matter. Predictions of alternative scenarios for the satellites' origin with the relevant observational constraint are compared. The orbital evolution of the larger satellites of Uranus is discussed to gain an understanding of their present orbital properties and possibly important past tidal heating episodes.

Pollack, James B.↗