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At least 145 records · Page 8

Effects of Saccharomyces cerevisiae quorum sensing signal molecules on ethanol production in bioethanol fermentation process

Here in this study, the concentrations of Saccharomyces cerevisiae quorum sensing signal molecules (QSMs) were determined, not to mention the exploration of the effects of exogenous S. cerevisiae QSMs on the sole fermentation of S. cerevisiae and co-fermentation of S. cerevisiae and Lactobacillus plantarum. The results showed that the concentrations of three signal molecules (2-phenylethanol (2-PE), tyrosol and tryptophan) produced by S. cerevisiae increased with a higher bacteria density, which tends to become stable up to 118.02, 32.05 and 1.93 mg/L respectively when cultivating for 144 h. Among the three signaling molecules, only 2-PE promoted the ethanol production capacity of S. cerevisiae. The ethanol concentration of the sole fermentation of S. cerevisiae loaded with 120 mg/L 2-PE reached 3.2 g/L in 9 h, which was 58.7% higher than that of the group without 2-PE addition. Moreover, 2-PE reduced the negative impact of L. plantarum on S. cerevisiae. Within 12 h of the co-fermentation of L. plantarum and S. cerevisiae, the ethanol concentration in the co-fermentation group loaded with 2-PE reached 5.6 g/L, similar to that in the group fermenting with sole S. cerevisiae, and the yeast budding rate was also restored to 28.51%. qRT-PCR results of S. cerevisiae which was in sole fermentation with 2-PE addition for 9 h showed that the relative expression levels of ethanol dehydrogenase gene ADH1 in S. cerevisiae decreased by 25% and the relative expression levels of MLS1, CIT2, IDH1,CIT1 decreased by 26%, 30%, 22%,18%, respectively, meant that the glyoxylic and tricarboxylic acid cycles were greatly inhibited, which promotes the accumulation of ethanol. The results of this study provide basic data for using QSMs more than antibiotics in the the prevention of contamination during the industrialized bioethanol production.

2-phenylethanol↗

Spiral Orbit Tribometer

The spiral orbit tribometer (SOT) bridges the gap between full-scale life testing and typically unrealistic accelerated life testing of ball-bearing lubricants in conjunction with bearing ball and race materials. The SOT operates under realistic conditions and quickly produces results, thereby providing information that can guide the selection of lubricant, ball, and race materials early in a design process. The SOT is based upon a simplified, retainerless thrust bearing comprising one ball between flat races (see figure). The SOT measures lubricant consumption and degradation rates and friction coefficients in boundary lubricated rolling and pivoting contacts. The ball is pressed between the lower and upper races with a controlled force and the lower plate is rotated. The combination of load and rotation causes the ball to move in a nearly circular orbit that is, more precisely, an opening spiral. The spiral s pitch is directly related to the friction coefficient. At the end of the orbit, the ball contacts the guide plate, restoring the orbit to its original radius. The orbit is repeatable throughout the entire test. A force transducer, mounted in-line with the guide plate, measures the force between the ball and the guide plate, which directly relates to the friction coefficient. The SOT, shown in the figure, can operate in under ultra-high vacuum (10(exp -9) Torr) or in a variety of gases at atmospheric pressure. The load force can be adjusted between 45 and 450 N. By varying the load force and ball diameter, mean Hertzian stresses between 0.5 and 5.0 GPa can be obtained. The ball s orbital speed range is between 1 and 100 rpm.

Pepper, Stephen V.↗

Voltage Restoration in Microgrids using Temporal Logic Specifications

This paper proposes an energy storage controller synthesis method for voltage restoration in microgrids with respect to temporal logic specifications (TLSs). TLSs is introduced in this paper as a formalism to control the voltage variation of a critical bus against an operational bounds over time. The power system with synchronous generator (SG) connected to a critical load bus is modeled as a set of differential-algebraic equations and a simplified analytical model is derived to describe the voltage variation of this critical bus. The control objective is to schedule an optimal control input signal from a supportive energy storage system (ESS) connected to the critical bus, such that the voltage variation of the latter satisfies the TLSs, such as a finite-time restoration. The proposed control is verified on a lumped distribution system model. With this control diagram, supportive controllers can be designed to make voltage behaviors comply with grid codes and avoid unnecessary relay actions.

Taousser, Fatima Zohra↗

Decentralized Distribution System Restoration with Grid-Forming/Following Inverter-Based Resources

The high penetration of distributed energy resources (DERs) in active distribution systems has posed challenges to the centralized distribution system restoration (DSR) strategies in current practice. On the other hand, the advancement in smart inverter technologies enables the bottom-up restoration capability. This paper is motivated to develop a 3-layered hierarchical framework for decentralized DSR, based on the grid-forming (GFM) and grid-following (GFL) grid-edge inverters. The first layer presents the tertiary control, which determines the load pickup schedule and generation dispatch of DERs, using the alternating direction method of the multipliers algorithm. The second layer consists of two control functions: GFM control, which regulates voltage and frequency, establishing a stable grid for GFL inverters to follow; and GFL control, which regulates the real and reactive power. In the third layer, the primary control is proposed to regulate the inverter voltage and current, which is developed based on the virtual oscillator control (VOC). Furthermore, the developed framework is tested in the modified IEEE 13-node test feeder. Two scenarios of grid-connected and islanded operating modes are designed, and simulation results demonstrate the effectiveness of decentralized DSR strategies for controlling grid-edge inverters to enhance the distribution system resilience.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Fault Diagnosis of Power Systems Using Intelligent Systems

The power system operator's need for a reliable power delivery system calls for a real-time or near-real-time Al-based fault diagnosis tool. Such a tool will allow NASA ground controllers to re-establish a normal or near-normal degraded operating state of the EPS (a DC power system) for Space Station Alpha by isolating the faulted branches and loads of the system. And after isolation, re-energizing those branches and loads that have been found not to have any faults in them. A proposed solution involves using the Fault Diagnosis Intelligent System (FDIS) to perform near-real time fault diagnosis of Alpha's EPS by downloading power transient telemetry at fault-time from onboard data loggers. The FDIS uses an ANN clustering algorithm augmented with a wavelet transform feature extractor. This combination enables this system to perform pattern recognition of the power transient signatures to diagnose the fault type and its location down to the orbital replaceable unit. FDIS has been tested using a simulation of the LeRC Testbed Space Station Freedom configuration including the topology from the DDCU's to the electrical loads attached to the TPDU's. FDIS will work in conjunction with the Power Management Load Scheduler to determine what the state of the system was at the time of the fault condition. This information is used to activate the appropriate diagnostic section, and to refine if necessary the solution obtained. In the latter case, if the FDIS reports back that it is equally likely that the faulty device as 'start tracker #1' and 'time generation unit,' then based on a priori knowledge of the system's state, the refined solution would be 'star tracker #1' located in cabinet ITAS2. It is concluded from the present studies that artificial intelligence diagnostic abilities are improved with the addition of the wavelet transform, and that when such a system such as FDIS is coupled to the Power Management Load Scheduler, a faulty device can be located and isolated from the rest of the system. The benefit of these studies provides NASA with the ability to quickly restore the operating status of a space station from a critical state to a safe degraded mode, thereby saving costs in experimentation rescheduling, fault diagnostics, and prevention of loss-of-life.

Momoh, James A.↗

OSAM-2: Plans and Progress for the First Demonstration of Structural Manufacturing in Space

OSAM-2 began as a NASA Space Technology Mission Directorate Tipping Point project in 2016 in which an investment made in ground development and demonstration and/or flight demonstration will result in: Significant advancement of technology’s maturation High likelihood for utilization of technology in commercially fielded space application Significant improvement in ability to successfully bring space technology to market Topic area was “Robotic In-Space Manufacturing and Assembly of Spacecraft and Space Structures” Made In Space, Inc. (now Redwire) proposed Archinaut One, an in-space robotic precision manufacturing and assembly system for larger-than-deployable structures Manufacturing / assembly in the operational environment allows manufactured parts to be designed for that environment (and not for launch loads and need to deploy) In 2020 NASA budget language, NASA began using On-orbit Servicing, Assembly, and Manufacturing Mission 2 (OSAM-2) for Archinaut One Restore-L + SPIDER (SPace Infrastructure DExterous Robot) became OSAM-1 Note: Slide 7 contains video animation, best viewed in presentation mode, run time 2 mins 25 secs.

In-Space Manufacturing↗

Regional Power System Black Start with Run-of-river Hydropower Plant and Battery Energy Storage

Battery energy storage systems (BESSs) are an important asset for power systems with high integration levels of renewable energy, and they can be controlled to provide various critical services to the power grid. This paper presents the real-world experience of using a megawatt-scale BESS with grid-following (GFL) and grid-forming (GFM) controls and a run-of-river (ROR) hydropower plant to restore a regional power system. To demonstrate this, we carry out power-hardware-in-the-loop experiments integrating an actual GFL- or GFM-controlled BESS and a load bank. Both the simulation and experimental results presented in this paper show the different roles of GFL- or GFM-controlled BESS in power system black starts. The results provide further insight for system operators on how GFL- or GFM-controlled BESS can enhance grid stability and how an ROR hydropower plant can be converted into a black-start-capable unit with the support of a small-capacity BESS. The results show that an ROR hydropower plant combined with a BESS has the potential of becoming one of enabling elements to perform bottom-up black-start schemes as opposed to conventional bottom-down method, thus enhancing the system resiliency and robustness.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Addressing Coldwater Temperature Impairment in a Changing Climate

Climate change is concerning for fisheries professionals because increased water temperatures threaten coldwater habitats, which are vital to recreational and commercial fisheries and keystone species. The Clean Water Act provides a legal framework to identify and address water quality impairments including those associated with temperature. However, although this framework provides a basic strategy to identify impairments, develop total maximum daily loads, and reduce pollution, it does not explicitly consider the challenges of a changing climate in addressing water quality impairments. Clean Water Act administrators and fisheries professionals can facilitate temperature restoration planning and implementation using the Clean Water Act framework to link stakeholders to the management of coldwater resources. Stakeholder engagement and collaboration, robust local monitoring, and incorporating climate resilience into restoration planning and implementation will promote comprehensive management of temperature impairments and the effects of climate change on fisheries.

54 ENVIRONMENTAL SCIENCES↗

Using Distributed Dynamic Voltage Restorers to Improve the Frequency Stability of Low-Inertia Networked Microgrids

This paper elucidates the novel concept of using distributed dynamic voltage restorers (DVRs), which are traditionally used to compensate for voltage sags, to improve the frequency stability of low-inertia networked microgrids. During frequency transients, the distributed DVRs rapidly regulate load voltages within an acceptable range, resulting in a fast change of load power consumption and helping improve frequency stability. To enable large-scale network simulation, a phasor-based model of DVRs is developed and validated against real data from a commercially available device. Finally, simulation results of a modified IEEE 123-node test feeder that comprises three microgrids and six DVRs verify the effectiveness of the proposed method.

Du, Wei↗

Quantifying the Protectability of Power Systems for Restoration Applications

With the increasing availability of distributed energy resources, controllable switches, and advanced metering infrastructure, distribution systems are exploring opportunities for improving system reliability through fault location, isolation, and service restoration. During permanent outages, this requires islanding microgrids and/or reconfiguration of the feeders; however, it is critical to assess the protection of the islanded/reconfigured networks before transition because the rated load and fault current conditions change. This paper presents a novel “protectability” metric that quantifies the protection performance and allows the operators to compare the effectiveness of the protection between two or more possible reconfiguration and islanding approaches. We present the mathematical formulation and assumptions for the metric. Multiple configurations of the Electric Power Research Institute J1 feeder, both in grid-connected and weak-sourced islanded operation, are used to demonstrate the performance of the metric with reference to overcurrent protection. Takeaways for future studies on system planning integration are provided.

distributed energy resources↗

Advanced Stirling Convertor Control Unit Testing at NASA Glenn Research Center in the Radioisotope Power Systems System Integration Laboratory

Future NASA missions could include long-duration flyby, orbital, lander, or rover applications where generating power from sunlight may be limited. Radioisotope power systems (RPSs) provide a dependable power source for missions where inadequate sunlight or operational requirements make other power systems impractical. Over the past 16 years, NASA Glenn Research Center has been supporting the development of RPSs. The advanced Stirling radioisotope generator (ASRG) utilized a pair of advanced Stirling convertors (ASCs). Although flight development of the ASRG has been canceled, much of the technology and hardware continued development and testing to guide future activities. Specifically, a controller for the convertor(s) is an integral part of a Stirling-based RPS. For the ASRG design, the controller maintains stable operation of the convertors, regulates the alternating current produced by the linear alternator of the convertor, provides a specified direct-current output voltage for the spacecraft, and synchronizes the piston motion of the two convertors to minimize vibration as well as manage and maintain operation with a stable piston amplitude and hot-end temperature. It not only provides power to the spacecraft but also must regulate convertor operation to avoid damage to internal components and maintain safe thermal conditions after fueling. Lockheed Martin Coherent Technologies, Inc., has designed, developed, and tested an ASC control unit engineering development unit (ACU EDU) to support this effort. GRC used the ACU EDU as part of its nonnuclear representation of a RPS that also consists of a Dual advanced Stirling convertor simulator (DASCS), and associated support equipment to perform a test in the Radioisotope Power Systems System Integration Laboratory (RSIL). The RSIL was designed and built with flexibility to evaluate hardware utilizing RPS technology. The RSIL provides insight into the electrical interactions between as many as three radioisotope power generators, associated control strategies, and typical electric system loads. The first phase of testing included a DASCS that was developed by Johns Hopkins University Applied Physics Laboratory and simulates the operation and electrical behavior of a pair of ASCs in real time via a combination of hardware and software. Testing included the following spacecraft electrical energy storage configurations: capacitor, battery, and supercapacitor. Testing of the DASCS and ACU in each energy storage configuration included simulation of a typical mission profile and transient voltage and current data during load turnon and turnoff. Testing for these devices also included the initiation of several system faults such as short circuits, electrical bus overvoltage, undervoltage, and a "dead bus" recovery to restore normal power operations. The goal of this testing was to verify operation of the ACU(s) when connected to a spacecraft electrical bus. The results of these tests are presented here.

General↗

Community Resilience Through Rapid Restoration Leveraging Distributed Energy Resources (DERs) and Low-Cost Sensors

Equitable and automated bottoms-up power restoration following an extreme event will be demonstrated at a site in Puerto Rico. To do so, the team will develop enhanced grid situational awareness techniques integrating behind-the-meter (BTM) distributed energy resources (DER) discovery, impedance sweeping based outage boundary detection, and feasible restoration path identification algorithms. Resilience metric will be developed and incorporated along with situational awareness information in a distributed Model Predictive Control (MPC)-based restoration optimization algorithm to control and mobilize grid assets. These algorithms will be validated through power hardware-in-the-loop experiments and ultimately, a site demonstration to show that outage recovery time and total recovered load could be improved by >20% over the baseline.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Torque control for electric motors

Method for adjusting electric-motor torque output to accomodate various loads utilizes phase-lock loop to control relay connected to starting circuit. As load is imposed, motor slows down, and phase lock is lost. Phase-lock signal triggers relay to power starting coil and generate additional torque. Once phase lock is recoverd, relay restores starting circuit to its normal operating mode.

Bernard, C. A.↗

An Irreversible Constitutive Law for Modeling the Delamination Process Using Interface Elements

An irreversible constitutive law is postulated for the formulation of interface elements to predict initiation and progression of delamination in composite structures. An exponential function is used for the constitutive law such that it satisfies a multi-axial stress criterion for the onset of delamination, and satisfies a mixed mode fracture criterion for the progression of delamination. A damage parameter is included to prevent the restoration of the previous cohesive state between the interfacial surfaces. To demonstrate the irreversibility capability of the constitutive law, steady-state crack growth is simulated for quasi-static loading-unloading cycle of various fracture test specimens.

Goyal, Vinay K.↗

An Irreversible Constitutive Law for Modeling the Delamination Process using Interface Elements

An irreversible constitutive law is postulated for the formulation of interface elements to predict initiation and progression of delamination in composite structures. An exponential function is used for the constitutive law such that it satisfies a multi-axial stress criterion for the onset of delamination, and satisfies a mixed mode fracture criterion for the progression of delamination. A damage parameter is included to prevent the restoration of the previous cohesive state between the interfacial surfaces. To demonstrate the irreversibility capability of the constitutive law, steady-state crack growth is simulated for quasi-static loading-unloading cycle of various fracture test specimens.

Goyal, Vinay K.↗

Development of lower body negative pressure as a countermeasure for orthostatic intolerance

Exposure to prolonged (1-4 hr) lower body negative pressure (LBNP) is a countermeasure against postflight orthostatic intolerance which is used in the Soviet space program and planned for use in the American space program. LBNP in combination with fluid-loading is believed to act by promoting a transient positive fluid balance resulting in an increase in vascular, as well as extravascular fluid. Inflight LBNP also may provide beneficial orthostatic effects by restoring baroreceptor reflex functions and/or lower body venous compliance. Current research efforts at the Johnson Space Center are directed toward increasing the effectiveness and efficiency of the LBNP and saline countermeasure. A promising avenue may involve combining pharmacologic agents, such as inhaled anti-diuretic hormone, or mineralocorticoids, with mechanical stimuli such as LBNP.

Fortney, Suzanne M.↗

Intralaminar and Interlaminar Progressive Failure Analysis of Composite Panels with Circular Cutouts

A progressive failure methodology is developed and demonstrated to simulate the initiation and material degradation of a laminated panel due to intralaminar and interlaminar failures. Initiation of intralaminar failure can be by a matrix-cracking mode, a fiber-matrix shear mode, and a fiber failure mode. Subsequent material degradation is modeled using damage parameters for each mode to selectively reduce lamina material properties. The interlaminar failure mechanism such as delamination is simulated by positioning interface elements between adjacent sublaminates. A nonlinear constitutive law is postulated for the interface element that accounts for a multi-axial stress criteria to detect the initiation of delamination, a mixed-mode fracture criteria for delamination progression, and a damage parameter to prevent restoration of a previous cohesive state. The methodology is validated using experimental data available in the literature on the response and failure of quasi-isotropic panels with centrally located circular cutouts loaded into the postbuckling regime. Very good agreement between the progressive failure analyses and the experimental results is achieved if the failure analyses includes the interaction of intralaminar and interlaminar failures.

Goyal, Vinay K.↗

Countermeasures for Mitigation of Sensorimotor Decrements Following Head-Down Bed Rest

BACKGROUND Astronauts experience postflight disturbances in postural and locomotor control due to sensorimotor adaptations during spaceflight. These alterations may have adverse consequences if a rapid egress is required after landing. Although exercise is partially effective for mitigating cardiovascular and muscular deconditioning, additional countermeasures are needed to further preserve sensorimotor function for exploration missions. We have identified proprioceptive training and electrical muscle stimulation (EMS) as promising in-flight countermeasures. Since prolonged head down bed rest (HDBR) is a spaceflight analog for body unloading and causes postural and locomotor control decrements that parallel those observed after spaceflight, it can be used to facilitate the development of these countermeasures. METHODS This study will determine the effects of proprioceptive training and EMS on functional task performance and sensorimotor function following 60 days of 6° HDBR. Subjects will be randomly assigned to one of four groups: 1)an EMS arm, 2) a proprioceptive training arm, 3) an exercise plus proprioceptive training arm, and 4) a control arm. The EMS countermeasure will include daily bilateral stimulation of selected bilateral lower extremity muscles (30 minutes per session). Proprioceptive training will be performed three days per week (20 minutes per session) consisting of body-loaded postural tasks in the horizontal position on an air bearing sled. Exercise training will mimic current protocols used on the International Space Station, but treadmill aerobic exercise will be replaced with additional cycling aerobic exercise. Primary outcome measures will include pre and post HDBR functional tests that are representative of high priority exploration mission tasks and require high demand for dynamic control of postural stability. Additional measures will be used to identify the key physiological factors contributing to countermeasure benefits. Given the constrained samples size, a Bayesian modelling approach will be used to quantify the probability that there is an effect of a given magnitude. HARDWARE AND PROTOCOL DEVELOPMENT Proprioceptive countermeasure design enhancements continued as part of the Mars Campaign Office (MCO) Crew Health and Performance (CHP) Crew Health Countermeasures (CHC). The primary goals of this work were to assemble a portable version of the countermeasure system that can be used at the :envihab facility, expand the software feedback system’s capabilities, and develop an actuator loading system that mimics what could be used on the International Space Station. In addition, one major piece of exercise hardware used in previous HDBR studies, the Horizontal Squat Device, was reassembled and restored to full functionality. Human-in-the-loop pilot testing is ongoing, prior to shipping both devices to the :envihab HDBR facility. Evaluations are also underway for the best EMS device technologies and specific methods. Finally, assessment techniques are being translated for administration in the horizontal position (e.g., leg dexterity and foot sole skin sensitivity). Our current goals are to refine the countermeasure training and assessment techniques and integrate protocols across multiple modalities. RELEVANCE The deliverable from this project will be proof-of-concept sensorimotor countermeasure designs for functional task performance with full assessment of efficacy in a spaceflight analog. If one or more countermeasures are effective, they will be translated for validation with the suite of operationally implemented in-flight countermeasures. ACKNOWLEDGEMENT This work is supported by NASA’s Human Research Program Human Health Countermeasures Element and by the Canadian Space Agency (L. Bent).

T R Macaulay↗