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At least 145 records · Page 8
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

Flux REaction TArget Prioritization (Flux RETAP) v1

Metabolic engineering is evolving rapidly as a result of new advances in synthetic biology and automation, as well as the irruption of machine learning (ML). ML has been shown to provide the predictive power synthetic biology lacked and needed, and to be able to effectively guide the metabolic engineering process. However, current technical limitations prevent the independent application of ML approaches to metabolic engineering without the use of previous biological knowledge in the form of a prioritized list of desirable engineering targets. Here, we present FluxRETAP, a simple and computationally inexpensive method that leverages the prior mechanistic knowledge embedded in genome-scale metabolic models (GSMs) for suggesting targets for genetic overexpression, downregulation or deletion, with the final goal of increasing metabolite production. FluxRETAP captured 100% of reaction targets experimentally verified to improve Escherichia coli isoprenol production in the literature accessible to us, 50% of targets that experimentally improved taxadiene production in E. coli and ~60% of genetic targets from a verified minimal constrained cut-set in Pseudomonas putida while providing additional high priority targets that could be tested. Overall, FluxRETAP is an efficient algorithm for identifying a prioritized list of testable genetic and reaction targets which can also be utilized in ML pipelines.

Czajka, Jeffrey [Battelle Memorial Institute, Paci↗
NASA NTRS1985

An Idealized Short Period Comet Model: Surface Insolation, H2O Flux, Dust Flux and Mantle Evolution

The present cometary activity model integrates feedback processes that involve the transport of heat, gas, and dust, as well as dust mantle development, and includes the effects of latitude, rotation, and spin orientation. Attention is given to the development, structural change, and distribution of dust mantles and their mutual interaction with ice surface temperature and gas and dust production. The results obtained suggest that an initially homogeneous, short period comet with a cosmic dust to water ice ratio, as well as typical orbit, rotation rate, and grain size distribution, would develop only a thin, less than 1 mm cyclic mantle at all points on the nucleus. Most H2O dust histories deduced from brightness data are noted to be in reasonable agreement with the model, allowing for uncertainty in radius and albedo. The exceptional case of Comet Encke is discussed.

Fanale, F. P.↗
NASA NTRS1984

An idealized short-period comet model - Surface insolation, H2O flux, dust flux, and mantle evolution

The present cometary activity model integrates feedback processes that involve the transport of heat, gas, and dust, as well as dust mantle development, and includes the effects of latitude, rotation, and spin axis orientation. Attention is given to the development, structural change, and distribution of dust mantles and their mutual interaction with ice surface temperature and gas and dust production. The results obtained suggest that an initially homogeneous, short period comet with a cosmic dust-to-water ice ratio, as well as typical orbit, rotation rate, and grain size distribution, would develop only a thin, less than 1 mm cyclic mantle at all points on the nucleus. Most H2O dust histories deduced from brightness data are noted to be in reasonable agreement with the model, allowing for uncertainty in radius and albedo. The exceptional case of Comet Encke is discussed.

Fanale, F. P.↗
NASA NTRS1989

Substorms, plasmoids, flux robes, and magnetotail flux loss on March 25, 1983 - CDAW-8

During a 9-hour period following a storm-sudden commencement, six spacecraft near geosynchronous orbit, one over the pole, and three in the mgnetotail, monitored a complex sequence of magnetospheric variations. Magnetic field compressions associated with the sudden commencement were seen first by the near-earth spacecraft and subsequently by the three down-tail spacecraft with increasing time delays that were consistent with the tailward movement of an interplanetary-shock-associated pressure enhancement. Ground magnetograms and synchronous orbit data are used to identify 7 substorm intensifications during this geomagnetically active period. Six of these intensifications are clearly associated with tail lobe field decreases about 18 R sub E behind the earth. Four of these intensifications are followed by both Bz field increases in the tail lobes at about 18 and about 30 R sub E and by the subsequent observation of rapidly flowing plasma sheet plasma at ISEE 3 about 110 R sub E down the tail. During two substorms where DE 1 was optically observing the auroral oval, the area of the polar cap was observed to decrease as the tail lobe field decreased at 18 R sub E. All these observations are consistent with the substorm associated release of a plasmoid at a neutral line near 20 R sub E.

Fairfield, D. H.↗
NASA NTRS1990

Evaluation of sensible heat flux, latent heat flux, and effective aerodynamic roughness at the land surface from a combination of satellite and in-situ data

A simple method is presented for using the daytime rise of the PBL height, combined with coincident satellite skin temperature measurements, to evaluate sensible heating and the effective surface roughness for heat transfer at synoptic locations. Multiple runs of a combination surface layer-mixed layer model are made in the central U.S., varying the values of surface moisture and roughness length around climatological means, and initializing the model with a set of atmospheric conditions from the synoptic report. The results, shown in figures and tables, are quite reasonable and spatially coherent, and depict well the sharp change in surface moisture and roughness which is known to take place across the central U.S.

Diak, George R.↗