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At least 145 records · Page 8

Metal-Templated Design of Chemically Switchable Protein Assemblies with High-Affinity Coordination Sites

To mimic a hypothetical pathway for protein evolution, we previously tailored a monomeric protein (cyt cb562) for metal-mediated self-assembly, followed by re-design of the resulting oligomers for enhanced stability and metal-based functions. We show that a single hydrophobic mutation on the cyt cb562 surface drastically alters the outcome of metal-directed oligomerization to yield a new trimeric architecture, (TriCyt1)3. This nascent trimer was redesigned into second and third-generation variants (TriCyt2)3 and (TriCyt3)3 with increased structural stability and preorganization for metal coordination. The three TriCyt variants combined furnish a unique platform to 1) provide tunable coupling between protein quaternary structure and metal coordination, 2) enable the construction of metal/pH-switchable protein oligomerization motifs, and 3) generate a robust metal coordination site that can coordinate all mid-to-late first-row transition-metal ions with high affinity.

Albert Kakkis↗

Differential Saccade-Pursuit Coordination Under Sleep Loss and Low-Dose Alcohol

Introduction: Ocular tracking of a moving object requires tight coordination between smooth pursuit and saccadic eye movements. Normally, pursuit drives gaze velocity to closely match target velocity, with residual position offsets corrected by catch-up saccades. However, how/if common stressors affect this coordination is largely unknown. This study seeks to elucidate the effects of acute and chronic sleep loss, and low-dose alcohol, on saccade-pursuit coordination, as well as that of caffeine. Methods: We used an ocular tracking paradigm to assess three metrics of tracking (pursuit gain, saccade rate, saccade amplitude) and to compute “ground lost” (from reductions in steady-state pursuit gain) and “ground recouped” (from increases in steady-state saccade rate and/or amplitude). We emphasize that these are measures of relative changes in positional offsets, and not absolute offset from the fovea. Results: Under low-dose alcohol and acute sleep loss, ground lost was similarly large. However, under the former, it was nearly completely recouped by saccades, whereas under the latter, compensation was at best partial. Under chronic sleep restriction and acute sleep loss with a caffeine countermeasure, the pursuit deficit was dramatically smaller, yet saccadic behavior remained altered from baseline. In particular, saccadic rate remained significantly elevated, despite the fact that ground lost was minimal. Discussion: This constellation of findings demonstrates differential impacts on saccade-pursuit coordination with low-dose alcohol impacting only pursuit, likely through extrastriate cortical pathways, while acute sleep loss not only disrupts pursuit but also undermines saccadic compensation, likely through midbrain/brainstem pathways. Furthermore, while chronic sleep loss and caffeine-mitigated acute sleep loss show little residual pursuit deficit, consistent with uncompromised cortical visual processing, they nonetheless show an elevated saccade rate, suggesting residual midbrain and/or brainstem impacts.

smooth pursuit↗

Working Toward A National Coordinated Soil Moisture Monitoring Network: Vision, Progress, and Future Directions

Soil moisture is a critical land surface variable, impacting the water, energy, and carbon cycles. While in situ soil moisture monitoring networks are still developing, there is no cohesive strategy or framework to coordinate, integrate, or disseminate these diverse data sources in a synergistic way that can improve our ability to understand climate variability at the national, state, and local levels. Thus, a national strategy is needed to guide network deployment, sustainable network operation, data integration and dissemination, and user-focused product development. The National Coordinated Soil Moisture Monitoring Network (NCSMMN) is a federally led, multi-institution effort that aims to address these needs by capitalizing on existing wide-ranging soil moisture monitoring activities, increasing the utility of observational data, and supporting their strategic application to the full range of decision-making needs. The goals of the NCSMMN are to 1) establish a national “network of networks” that effectively demonstrates data integration and operational coordination of diverse in situ networks; 2) build a community of practice around soil moisture measurement, interpretation, and application—a “network of people” that links data providers, researchers, and the public; and 3) support research and development (R&D) on techniques to merge in situ soil moisture data with remotely sensed and modeled hydrologic data to create user-friendly soil moisture maps and associated tools. The overarching mission of the NCSMMN is to provide coordinated high-quality, nationwide soil moisture information for the public good by supporting applications like drought and flood monitoring, water resource management, agricultural and forestry planning, and fire danger ratings.

C. Bruce Baker↗

Pathways to International Coordination in Space Weather

The United Nations Committee for the Peaceful Uses of Outer Space(UN-COPUOS) in its 2022 Space Weather Expert Group report, called upon the World Meteorological Organization (WMO), the InternationalSpace Environment Service (ISES) and the Committee on Space Research (COSPAR) to take a leadership role in improving the global coordination of space weather activities in consultation and collaboration with other relevant actors and international organizations. The three organizations have established a framework for trusting partnership while minimizing duplication of efforts and have agreed on three distinct domains in which the particular expertise and strengths of each of the three were identified: Research and Development (COSPAR), Facilitating Integration (WMO), andServices (ISES). The next stage in building a pathway to improved coordination is to lay the same foundation with other relevant organizations and actors engaged in space weather. We will present outcomes of the first International Space Weather Coordination Forum intending to shape the future of international collaboration on space weather with the ultimate aim of increasing the community’s ability to mitigate space environment threats. We will discuss approaches to alignments of bottom-up initiatives and community-driven roadmaps with national/regional strategic planning activities and funding programs. We will also review opportunities for taking advantage ofInternational Space Weather Action Teams (ISWAT) for planning pilot projects to demonstrate the value of collaboration and coordination.

Maria M Kuznetsova↗

Application of the generalized vertical coordinate ocean model for better representing satellite data

It is found that two adaptive parametric functions can be introduced into the basic ocean equations for utilizing the optimal or hybrid features of commonly used z-level, terrain- following, isopycnal, and pressure coordinates in numerical ocean models. The two parametric functions are formulated by combining three techniques: the arbitrary vertical coordinate system of Kasahara (1 974), the Jacobian pressure gradient formulation of Song (1 998), and a newly developed metric factor that permits both compressible (non-Boussinesq) and incompressible (Boussinesq) approximations. Based on the new formulation, an adaptive modeling strategy is proposed and a staggered finite volume method is designed to ensure conservation of important physical properties and numerical accuracy. Implementation of the combined techniques to SCRUM (Song and Haidvogel1994) shows that the adaptive modeling strategy can be applied to any existing ocean model without incurring computational expense or altering the original numerical schemes. Such a generalized coordinate model is expected to benefit diverse ocean modelers for easily choosing optimal vertical structures and sharing modeling resources based on a common model platform. Several representing oceanographic problems with different scales and characteristics, such as coastal canyons, basin-scale circulation, and global ocean circulation, are used to demonstrate the model's capability for multiple applications. New results show that the model is capable of simultaneously resolving both Boussinesq and non-Boussinesq, and both small- and large-scale processes well. This talk will focus on its applications of multiple satellite sensing data in eddy-resolving simulations of Asian Marginal Sea and Kurosio. Attention will be given to how Topex/Poseidon SSH, TRMM SST; and GRACE ocean bottom pressure can be correctly represented in a non- Boussinesq model.

numerical ocean model generalized coordinate syste↗

Lessons Learned from Coordinating Relay Activities at Mars

The Mission Management Office at the Jet Propulsion Laboratory was tasked with coordinating the relay of data between multiple spacecraft at Mars in support of the Mars Exploration Rover Missions in early 2004. The confluence of three orbiters (Mars Global Surveyor, Mars Odyssey, and Mars Express), two rovers (Spirit and Opportunity), and one lander (Beagle 2) has provided a challenging operational scenario that required careful coordination between missions to provide the necessary support and to avoid potential interference during simultaneous relay sessions. As these coordination efforts progressed, several important lessons were learned that should be applied to future Mars relay activities.

coordination↗

International Coordination and Cooperation on LunaNet Spectrum

LunaNet is planned to be the network of networks operated by a set of cooperating organizations to provide interoperable Communications, networking, Position, Navigation, and Timing (CPNT) services to users on and around the Moon based on a framework of mutually agreed-upon standards, protocols, frequency bands and interface requirements. LunaNet follows a service-oriented architecture that is agnostic about the types of organizations that provide services, e.g., government, industry, or academia. LunaNet is open, scalable, resilient, secure, and extensible. To achieve these goals, LunaNet Service Providers (LNSP) must coordinate with each other to define and develop the architecture, to plan initial and evolved capabilities, and to operate their networks. One of the central LunaNet tenets is the use of shared spectrum. For example, the Lunar Augmented Navigation Service (LANS) acts like a Global Navigation Satellite System (GNSS) such as the US Global Positioning System (GPS) or European Galileo but the LNSPs’ contributions to LunaNet must use the same frequency band (2483.5 MHz-2500.0 MHz) and transmit the same waveform synchronized by highly accurate clocks so that Users ‘see’ one virtual network and use the same multilateration algorithm to determine their positions. This necessitates a high degree of spectrum coordination. NASA’s Lunar and Human Spaceflight Spectrum Management Team has been actively supporting development of the LunaNet Interoperability Specification (LNIS), soliciting inputs from spectrum policy and planning experts across NASA, ESA and JAXA. Cislunar spectrum use considerations have been studied and adjudicated within the Space Frequency Coordination Group (SFCG) and inform the ongoing discussion of a lunar communication and navigation architecture within the existing radio regulatory framework of the International Telecommunication Union, leading to the 2027 World Radiocommunication Conference (WRC-27). The frequency plan contained in the publicly released draft of the LunaNet Interoperability Specification reflects the initial phase of exploration (roughly to 2030) defining an optimal set of radio frequencies in appropriately allocated services consistent with WRC-23 decisions for use by known or planned CPNT applications, while striving to maximize coexistence and compatibility amongst cislunar systems and other systems within the near-Earth regime (< 2 million km from Earth). Important considerations include: protection of extremely sensitive receive-only radio astronomy systems on the lunar far side, known as the Shielded Zone of the Moon (SZM); compatibility between Direct with Earth (DWE) communications links and links needed to support relay satellites in lunar orbit with their customer systems on orbit or on the lunar surface; compatibility between multiple lunar surface communications systems and capabilities over varied and challenging terrain and distances; as well as ensuring compatibility and interoperability between navigation systems which either leverage Earth-based or in-situ lunar systems. In addition, the lunar CPNT architecture is envisioned to be the basis – with adjustments – of the future Mars CPNT architecture as we expand into the solar system using Interplanetary Networking (IPN). The second phase of lunar spectrum definition will address planned international capabilities for the next decade that will require action at WRC-27 and beyond. This paper will discuss each of these considerations in more depth and how the current LunaNet frequency plan addresses them.

LunaNet↗

Empirical analytic transformations between geographic and corrected geomagnetic coordinates

Based upon a mathematical model of contours of constant corrected geomagnetic latitude in a polar projection of geographic coordinates, analytic equations are developed for converting geographic coordinates to corrected geomagnetic coordinates and vice versa. The equations were programmed for use on a small computer. This treatment is restricted to the Northern Hemisphere.

Comfort, R. H.↗

Coordinate perturbation and multiple scale in gasdynamics.

A simple way of applying the coordinate perturbation technique is developed which bypasses the usual transformations of the equations to perturbed coordinates, and which provides the appropriate coordinate stretching by simple algebraic manipulations on the nonuniformly valid expansion obtained by a straightforward expansion from the original equations. Interesting results are obtained by applying the procedure to two gasdynamical problems. In the first, the flowfield around a supersonic two-dimensional wing is determined up to third order, including a uniformly valid representation of the front shock shape. The second concerns the oscillations in a closed tube following an arbitrary initial disturbance, both when the two ends are closed, and when one of the two ends contains an oscillating piston.

Crocco, L.↗

Coordinate transformation by minimizing correlations between parameters

This investigation was to determine the transformation parameters (three rotations, three translations and a scale factor) between two Cartesian coordinate systems from sets of coordinates given in both systems. The objective was the determination of well separated transformation parameters with reduced correlations between each other, a problem especially relevant when the sets of coordinates are not well distributed. The above objective is achieved by preliminarily determining the three rotational parameters and the scale factor from the respective direction cosines and chord distances (these being independent of the translation parameters) between the common points, and then computing all the seven parameters from a solution in which the rotations and the scale factor are entered as weighted constraints according to their variances and covariances obtained in the preliminary solutions. Numerical tests involving two geodetic reference systems were performed to evaluate the effectiveness of this approach.

Kumar, M.↗

Coordinated adaptive washout for motion simulators.

This paper introduces a new method of providing motion cues to a moving base six-degree-of-freedom flight simulator utilizing nonlinear filters. Coordinated adaptive filters, used to coordinate translational and rotational motion, are derived based on the method of continuous steepest descent, and the basic concept of the digital controllers used for the uncoordinated heave and yaw cues is also presented. The coordinated adaptive washout method is illustrated by an application in a six-degree-of-freedom fixed-base environment.

Parrish, R. V.↗

Coordinate transformations for studies of interactions between interplanetary and geomagnetic fields

A graphical procedure is provided for performing coordinate transformations between the geocentric-solar-equatorial, geocentric-solar-ecliptic and geocentric-solar-magnetospheric coordinate systems. The procedure is designed to facilitate intercomparison of previous studies of interactions between interplanetary and geomagnetic fields that made use of these coordinate systems. The interaction in the geocentric-solar-magnetosphere system has been shown to give the most consistent results.

Burch, J. L.↗

Determination of selenographic coordinates of lunar surface points from single pictures obtained from Zond 6

Pictures of the lunar surface with an image of practically the entire lunar limb were obtained from the Zond 6 spacecraft. During the time of exposure, the entire lunar surface covered by these photographs was illuminated by the sun. Such single pictures were used to find the external orientation elements and selenographic coordinates of the photographed lunar surface points. The selenographic coordinate system was specified by the Goloseyev catalog and was realized by points of this catalog identified on the pictures and termed reference points. Craters located on the invisible side of the moon and also other points of the Goloseyev catalog, which could be used as control points, were taken as the points being determined. The technique used to compute the selenographic coordinates of the points is outlined.

Ziman, Y. L.↗

Station coordinates in the standard earth 3 system and radiation-pressure perturbations from ISAGEX camera data

Simultaneous and individual camera observations of GEOS 1, GEOS 2, Pageos, and Midas 4 obtained during the International Satellite Geodesy Experiment are utilized to determine station coordinates. The Smithsonian Astrophysical Observatory Standard Earth III system of coordinates is used to tie the geometrical network to a geocentric system and as a reference for calculating satellite orbits. A solution for coordinates combining geometrical and dynamical methods is obtained, and a comparison between the solutions and terrestrial data is made. The radiation-pressure and earth-albedo perturbations for Pageos are very large, and Pageos' orbits are used to evaluate the analytical treatment of these perturbations. Residual effects, which are probably of interest to aeronomists, remain in the Pageos orbits.

Gaposchkin, E. M.↗

Reformulation of the relativistic conversion between coordinate time and atomic time

The relativistic conversion between coordinate time and atomic time is reformulated to allow simpler time calculations relating analysis in solar system barycentric coordinates (using coordinate time) with earth-fixed observations (measuring 'earth-bound' proper time or atomic time). After an interpretation in terms of relatively well-known concepts, this simplified formulation, which has a rate accuracy of about 10 to the minus 15th, is used to explain the conventions required in the synchronization of a worldwide clock network and to analyze two synchronization techniques - portable clocks and radio interferometry. Finally, pertinent experimental tests of relativity are briefly discussed in terms of the reformulated time conversion.

Thomas, J. B.↗

Numerical solutions of the unsteady Navier-Stokes equations for arbitrary bodies using boundary-fitted curvilinear coordinates

A method of automatic body-fitted curvilinear coordinate generation is described and used to construct a finite-difference solution of the full incompressible time-dependent Navier-Stokes equations for the unsteady laminar viscous flow arbitrary two-dimensional airfoils or any other two-dimensional body. A method of controlling the spacing of the coordinate lines encircling the body is developed in order to treat high Reynolds number flows, since the coordinate lines must concentrate near the surface to a greater degree as the Reynolds number increases. Multiple airfoils and submerged hydrofoils are treated as illustrative examples. The solution shows good agreement with the Blasius boundary layer solution for the flow past a semi-infinite flat plate.

Thompson, J. F.↗

Nature of the requirements for reference coordinate systems /Review paper/

The current need for more precisely defined reference coordinate systems arises for geodynamics because the earth can certainly not be treated as a rigid body when measurement uncertainties reach the few-centimeter scale or its angular equivalent. At least two coordinate systems seem to be required. The first is a system defined in space relative to appropriate astronomical objects, suitable for ultimately expressing the dynamical equations of motion for the earth. The second coordinate system must be associated with the nonrigid earth in some well defined way so that the rotational motions of the whole earth are represented by the transformation parameters relating the earth system to the space-inertial system. The earth system should be defined so that the dynamical equations for relative motions of the various internal mechanical components of the earth and accurate measurements of these motions are conveniently expressed in this system.

Lundquist, C. A.↗