LCI1, a Chlamydomonas reinhardtii plasma membrane protein, functions in active CO 2 uptake under low CO 2
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A complex role for chlorine radicals Radicals are atoms or molecules that are highly reactive because they have an unpaired electron. A common means of investigating whether they are involved in a particular reaction is to try to trap them with an acceptor compound. Yang et al. reinvestigated a photoinduced alkane oxidation reaction for which a trapping study had previously implicated alkoxy radicals. Their spectroscopic, kinetic, and isotopic labeling studies revealed that chlorine, rather than alkoxy, was the key radical intermediate; the prior trapping results had stemmed from its complexation with alcohols. Science , abd8408, this issue p. 847
Background: Hydrogenases (H2ases) are metalloenzymes capable of the reversible conversion of protons and electrons to molecular hydrogen. Exploiting the unique enzymatic activity of H2ases can lead to advancements in the process of biohydrogen evolution and green energy production. Results: Here we created of a functional, optimized operon for rapid and robust production of recombinant [NiFe] Desulfomicrobium baculatum hydrogenase (Dmb H2ase). The conversion of the [NiFeSe] Dmb H2ase to [NiFe] type was performed on genetic level by site-directed mutagenesis. The native dmb operon includes two structural H2ase genes, coding for large and small subunits, and an additional gene, encoding a specific maturase (protease) that is essential for the proper maturation of the enzyme. Dmb, like all H2ases, needs intricate bio-production machinery to incorporate its crucial inorganic ligands and cofactors. Strictly anaerobic, sulfate reducer D. baculatum bacteria are distinct, in terms of their biology, from E. coli. Thus, we introduced a series of alterations within the native dmb genes. As a result, more than 100 elements, further compiled into 32 operon variants, were constructed. The initial requirement for a specific maturase was omitted by the artificial truncation of the large Dmb subunit. The assembly of the produced H2ase subunit variants was investigated both, in vitro and in vivo. This approach resulted in 4 recombinant [NiFe] Dmb enzyme variants, capable of H 2 evolution. The aim of this study was to overcome the gene expression, protein biosynthesis, maturation and ligand loading bottlenecks for the easy, fast, and cost-effective delivery of recombinant [NiFe] H2ase, using a commonly available E. coli strains. Conclusion: The optimized genetic constructs together with the developed growth and purification procedures appear to be a promising platform for further studies toward fully-active and O 2 tolerant, recombinant [NiFeSe] Dmb H2ase, resembling the native Dmb enzyme. It could likely be achieved by selective cysteine to selenocysteine substitution within the active site of the [NiFe] Dmb variant.
Red geysers are a population of massive (logM/M ⊙ ~ 10.5), quiescent galaxies with large-scale but weak, bisymmetric ionized gas outflows, interpreted as signatures of ongoing, low-level active galactic nucleus (AGN) feedback. We investigate the kinematics and prevalence of cool (T ∼ 100–1000 K) neutral gas traced by Na I D absorption, and its connection to galaxy environment and AGN activity. Using 140 red geyser galaxies from the Sloan Digital Sky Survey-IV Mapping Nearby Galaxies at Apache Point Observatory (or MaNGA), we measure spatially resolved velocities and dispersions via double-Gaussian fits to the Na I D doublet. We find that ∼70% of the cool gas is inflowing, with a median velocity of ∼47 km s −1 (∼10% of the expected freefall velocity), and that it also exhibits kinematically ordered motions with σ NaD /σ* ∼ 0.4. Additionally, the Na I D absorption is more prevalent in red geysers than in a matched control sample, with a higher detection fraction (63% versus 40%) and reservoir areas ∼1.6 times larger. Acceleration (∼1 Myr) and accretion (∼20 Myr) timescales indicate that the Na I D-absorbing clouds are likely young and short-lived. Another intriguing result is that radio-detected red geysers (30% of the sample) show inflowing gas reservoirs ∼7 times larger than in non-radio systems. Similarly, galaxies subject to environmental effects host inflowing gas reservoirs ∼2.7 times larger than isolated red geysers. We take this as evidence that galaxy environments play a key role in replenishing the cool gas reservoirs of red geysers, fueling central AGN activity, sustaining radio emission, and regulating long-term quiescence. These findings reveal that quiescent systems are governed by cycles of inflow, feedback, and regulation.
Comparison of observations of type III impulsive radio bursts made at the Clark Lake Radio Observatory with high-spatial-resolution cinematographic observations taken at the Big Bear Solar Observatory. Use of the log-periodic radio interferometer makes it possible to localize the radio emission uniquely. This study concentrates on the particularly active region close to the limb on May 22, 1970. Sixteen of the 17 groups were associated with some H alpha activity, 11 of them with the start of such activity.
The effects of fuselage cross section and structural arrangement on the performance of actively cooled hypersonic cruise vehicles are investigated. An active cooling system which maintains the aircraft's entire surface area at temperatures below 394 K at Mach 6 is developed along with a hydrogen fuel tankage thermal protection system. Thermodynamic characteristics of the actively cooled thermal protection systems established are summarized. Design heat loads and coolant flowrate requirements are defined for each major structural section and for the total system. Cooling system weights are summarized at the major component level. Conclusions and recommendations are included.
Study of the longitudinal magnetic field in a number of active limb prominences showing fields in excess of 30 G. The objects fall into three groups: surges, caps, and active-region prominences. There appears to be an upper limit of 150 to 200 G for the field strength in prominences. A model of surges is presented in which a presurge axisymmetric magnetic field is established by a line current in the corona. Particle acceleration in surges is observed, which indicates that V x B is not equal to zero in these objects during periods comparable to the Alfven transit time. The strong fields observed in caps seem to run between parts of active regions in accordance with Hale's law of sunspot group polarities.
It is pointed out that the autocorrelation coefficients of the daily Wolf sunspot numbers over a period of 128 years reveal a number of interesting features of the variability of solar activity. Besides establishing periodicities for the solar rotation, solar activity cycle, and, perhaps, the 'Gleissberg Cycle', they suggest that active longitudes do exist, but with much greater strength and persistence in some solar cycles than in others. Evidence is adduced for a variation in the solar rotation period, as measured by sunspot number, of as much as two days between different solar cycles.
The reduction of protein content in skeletal muscle undergoing disuse-induced atrophy is correlated with accelerated rates of protein degradation and reduced rates of protein synthesis (Goldspink, 1977). It is not known in what manner myofibers are partially disassembled during disuse atrophy to fibers of smaller diameter; nor is it known which proteases are responsible for this morphological change in contractile protein mass. Dayton and colleagues (1975) have suggested that the Ca(2+)-activated protease (CaP) may initiate myofibril degradation. The discovery of a form of CaP that is activatable by nano-molar concentrations of Ca(2+) indicates that CaP activity may be regulated by physiological concentrations of Ca(2+) (Mellgren, 1980). The enhancement of proteolysis by the Ca(2+) ionophore A23187, reported by Etlinger (1979), is consistent with a significant role for CaP in protein degradation. It was of interest, therefore, to measure the levels of CaP activity and the CaP inhibitor in extracts obtained from skeletal muscles of rat and chicken limbs undergoing disuse atrophy or stretch hypertrophy, respectively.
A group of comets defined as 'normal' by Cochran (1987) is compared with comets from which no C2 or C3 emissions have been detected and exhibit anomalously low activity. It is established that the low-activity comets are C2- and C3-depleted relative to CN, and that this depletion is not due to these comets' being low producers of all types of gases. A study of all the orbital parameters of the group of comets is conducted, but no parameter is found able to account for the observed difference between normal and low-activity types. Several alternative scenarios for the differences in question are proposed and discussed.
The general properties of elementary bipoles (EBs), the class of moving magnetic features identified by Frazier (1972) as building blocks of new solar active regions, are described, and variations in their characteristics are illustrated with extensive videomagnetograms obtained at Big Bear Solar Observatory during 1984-1989. Consideration is given to ephemeral active regions consisting of EBs with only one positive and one negative pole, multiple-pole ephemeral regions, reversed-polarity EBs, interactions among EBs and adjacent magnetic features, and the EBs of small and medium active regions. The detection of EBs prior to the appearance of arch filaments confirms the relationship found by Frazier.
In order to investigate how solar activity is organized in longitude, major solar flares, large sunspot groups, and large scale photospheric magnetic field strengths were analyzed. The results of these analyses are reported. The following results are discussed: hot spots, initially recognized as areas of high concentration of major flares, are the preferred locations for the emergence of big sunspot groups; double hot spots appear in pairs that rotate at the same rate separated by about 180 deg in longitude, whereas, single hot spots have no such companions; the northern and southern hemispheres behave differently in organizing solar activity in longitude; the lifetime of hot spots range from one to several solar cycles; a hot spot is not always active throughout its lifetime, but goes through dormant periods; and hot spots with different rotational periods coexist in the same hemisphere during the same solar cycle.
The objective of this effort is to develop an analytical model for the coupling of active noise control (ANC) piston-type actuators that are mounted flush to the inner and outer walls of an annular duct to the modes in the duct generated by the actuator motion. The analysis will be used to couple the ANC actuators to the modal analysis propagation computer program for the annular duct, to predict the effects of active suppression of fan-generated engine noise sources. This combined program will then be available to assist in the design or evaluation of ANC systems in fan engine annular exhaust ducts. An analysis has been developed to predict the modes generated in an annular duct due to the coupling of flush-mounted ring actuators on the inner and outer walls of the duct. The analysis has been combined with a previous analysis for the coupling of modes to a cylindrical duct in a FORTRAN computer program to perform the computations. The method includes the effects of uniform mean flow in the duct. The program can be used for design or evaluation purposes for active noise control hardware for turbofan engines. Predictions for some sample cases modeled after the geometry of the NASA Lewis ANC Fan indicate very efficient coupling in both the inlet and exhaust ducts for the m = 6 spinning mode at frequencies where only a single radial mode is cut-on. Radial mode content in higher order cut-off modes at the source plane and the required actuator displacement amplitude to achieve 110 dB SPL levels in the desired mode were predicted. Equivalent cases with and without flow were examined for the cylindrical and annular geometry, and little difference was found for a duct flow Mach number of 0.1. The actuator ring coupling program will be adapted as a subroutine to the cylindrical duct modal analysis and the exhaust duct modal analysis. This will allow the fan source to be defined in terms of characteristic modes at the fan source plane and predict the propagation to the arbitrarily-located ANC source plane. The actuator velocities can then be determined to generate the anti-phase mode. The resulting combined fan source/ANC pressure can then be calculated at any desired wall sensor position. The actuator velocities can be determined manually or using a simulation of a control system feedback loop. This will provide a very useful ANC system design and evaluation tool.
Super-active region NOAA 6555 was highly flare productive during the period March 21st - 27th, 1991 of its disk passage. We have studied its chromospheric activity using high spatial resolution H alpha filtergrams taken at Udaipur along with MSFC vector magnetograms. A possible relationship of flare productivity and the variation in shear has been explored. Flares were generally seen in those subareas of the active region which possessed closed magnetic field configuration, whereas only minor flares and/or surges occurred in subareas showing open magnetic field configuration. Physical mechanisms responsible for the observed surges are also discussed.
NASA Langley continues to aggressively investigate the potential advantages of active flow control over more traditional aerodynamic techniques. This paper provides an update to a previous paper and describes both the progress in the various research areas and the significant changes in the NASA research programs. The goals of the topics presented are focused on advancing the state of knowledge and understanding of controllable fundamental mechanisms in fluids as well as to address engineering challenges. An organizational view of current research activities at NASA Langley in active flow control as supported by several projects is presented. On-center research as well as NASA Langley funded contracts and grants are discussed at a relatively high level. The products of this research are to be demonstrated either in bench-top experiments, wind-tunnel investigations, or in flight as part of the fundamental NASA R&D program and then transferred to more applied research programs within NASA, DOD, and U.S. industry.
AIM: The vestibulosympathetic reflex refers to sympathetic nerve activation by the vestibular system. Animal studies indicate that the vestibular system assists in blood pressure regulation during orthostasis. Although human studies clearly demonstrate activation of muscle sympathetic nerve activity (MSNA) during engagement of the otolith organs, the role of the vestibulosympathetic reflex in maintaining blood pressure during orthostasis is not well-established. Examination of the vestibulosympathetic reflex with other cardiovascular reflexes indicates that it is a powerful and independent reflex. Ageing, which is associated with an increased risk for orthostatic hypotension, attenuates the vestibulosympathetic reflex. The attenuated reflex is associated with a reduction in arterial pressure. CONCLUSION: These findings suggest that the vestibulosympathetic reflex assists in blood pressure regulation in humans, but future studies examining this reflex in other orthostatically intolerant populations are necessary to address this hypothesis.
The Mars Exploration Rover mission depends on the Science Activity Planner as its primary interface to the Spirit and Opportunity Rovers. Scientists alternate between a series of mouse clicks and keyboard inputs to create a set of instructions for the rovers. To accelerate planning by minimizing mouse usage, a rover planning editor should receive the majority of inputted commands from the keyboard. Thorough investigation of the Eclipse platform's Java editor has provided the understanding of the base model for the Activity Scratchpad. Desirable Eclipse features can be mapped to specific rover planning commands, such as auto-completion for activity titles and content assist for target names. A custom editor imitating the Java editor's features was created with an XML parser for experimenting purposes. The prototype editor minimized effort for redundant tasks and significantly improved the visual representation of XML syntax by highlighting keywords, coloring rules, folding projections, and providing hover assist, templates and an outline view of the code.
The Soil Moisture Active Passive (SMAP) mission is one of the first-tier satellite missions recommended by the U.S. National Research Council Committee on Earth Science and Applications from Space. The SMAP mission 1 is under development by NASA and is scheduled for launch late in 2014. The SMAP measurements will allow global and high-resolution mapping of soil moisture and its freeze/thaw state at resolutions from 3-40 km. These measurements will have high value for a wide range of environmental applications that underpin many weather-related decisions including drought and flood guidance, agricultural productivity estimation, weather forecasting, climate predictions, and human health risk. In 2007, NASA was tasked by The National Academies to ensure that emerging scientific knowledge is actively applied to obtain societal benefits by broadening community participation and improving means for use of information. SMAP is one of the first missions to come out of this new charge, and its Applications Plan forms the basis for ensuring its commitment to its users. The purpose of this paper is to outline the methods and approaches of the SMAP applications activity, which is designed to increase and sustain the interaction between users and scientists involved in mission development.