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At least 145 records · Page 8

Probing transmembrane mechanical coupling and cytomechanics using magnetic twisting cytometry

We recently developed a magnetic twisting cytometry technique that allows us to apply controlled mechanical stresses to specific cell surface receptors using ligand-coated ferromagnetic microbeads and to simultaneously measure the mechanical response in living cells. Using this technique, we have previously shown the following: (i) beta 1 integrin receptors mediate mechanical force transfer across the cell surface and to the cytoskeleton, whereas other transmembrane receptors (e.g., scavenger receptors) do not; (ii) cytoskeletal stiffness increases in direct proportion to the level of stress applied to integrins; and (iii) the slope of this linear stiffening response differs depending on the shape of the cell. We now show that different integrins (beta 1, alpha V beta 3, alpha V, alpha 5, alpha 2) and other transmembrane receptors (scavenger receptor, platelet endothelial cell adhesion molecule) differ in their ability to mediate force transfer across the cell surface. In addition, the linear stiffening behavior previously observed in endothelial cells was found to be shared by other cell types. Finally, we demonstrate that dynamic changes in cell shape that occur during both cell spreading and retraction are accompanied by coordinate changes in cytoskeletal stiffness. Taken together, these results suggest that the magnetic twisting cytometry technique may be a powerful and versatile tool for studies analyzing the molecular basis of transmembrane mechanical coupling to the cytoskeleton as well as dynamic relations between changes in cytoskeletal structure and alterations in cell form and function.

NASA Discipline Number 40-20↗

Twist seal for high-pressure vessels such as space shuttle rocket motors

Seals for sealing clevis and flange joints (14) of a solid rocket booster motor, and more particularly to a seal (30) which is twisted upon application of expansion forces to an edge seal (36). This twisting motion initially causes a leading edge seal (44) to be urged into sealing engagement with a surface (48) of an adjacent member (20) and thereafter, increasing fluid pressure on a pressurized side (64) of a seal (30) drives a broad sealing region (46) into sealing engagement with a surface (48).

von Pragenau, George L.↗

Coupled CFD/CSD Computation of Airloads of an Active-Twist Rotor

An unsteady Reynolds averaged Navier-Stokes analysis loosely coupled with a comprehensive rotorcraft code for blade trim and aeroelastic effects is presented for a second-generation Active-Twist Rotor. Mesh and temporal sensitives of computed airloads are evaluated. In the final paper, computed airloads will be compared with wind tunnel data for the Active-Twist Rotor test that is currently underway.

Massey, Steven J.↗

Reconstruction of Twist Torque in Main Parachute Risers

The reconstruction of twist torque in the Main Parachute Risers of the Capsule Parachute Assembly System (CPAS) has been successfully used to validate CPAS Model Memo conservative twist torque equations. Reconstruction of basic, one degree of freedom drop tests was used to create a functional process for the evaluation of more complex, rigid body simulation. The roll, pitch, and yaw of the body, the fly-out angles of the parachutes, and the relative location of the parachutes to the body are inputs to the torque simulation. The data collected by the Inertial Measurement Unit (IMU) was used to calculate the true torque. The simulation then used photogrammetric and IMU data as inputs into the Model Memo equations. The results were then compared to the true torque results to validate the Model Memo equations. The Model Memo parameters were based off of steel risers and the parameters will need to be re-evaluated for different materials. Photogrammetric data was found to be more accurate than the inertial data in accounting for the relative rotation between payload and cluster. The Model Memo equations were generally a good match and when not matching were generally conservative.

Day, Joshua D.↗

Orion Capsule Parachute Assembly System (CPAS) Riser Twist Load Amplification

Any cluster of parachute systems is subject to effects on performance due to interactions between the parachutes. One such interaction is the twisting of a riser from one parachute around that of another. Due to friction and relative motion between the risers, it is possible for the tension in the riser near the attach point to be different from the tension in the riser towards the suspension lines or canopy. This could result in system failure due to larger than expected loading. The Orion Capsule Parachute Assembly System (CPAS) designed and executed a test to quantify the amplification of the load in a parachute riser due to twist, rocking rate and angle, cluster size, and canopy load. The design of the testing approach, test matrix, and hardware are discussed along with results and findings.

Parachute↗

A CME-Producing Solar Eruption from the Interior of a Twisted, Emerging Bipole

In a negative-polarity coronal hole, magnetic flux emergence, seen by the Solar Dynamics Observatory's (SDO) Helioseismic Magnetic Imager (HMI), begins at approximately 19:00 UT on March 3, 2016. The emerged magnetic field produced sunspots with penumbrae by 3:00 UT on March 4, which are a part of NOAA 12514. The emerging magnetic field is largely bipolar with the opposite-polarity fluxes spreading apart overall, but there is simultaneously some convergence and cancellation of opposite-polarity flux at the polarity inversion line (PIL) inside the emerging bipole. The emerging bipole shows obvious overall left-handed shear and/or twist in its magnetic field and corresponding clockwise rotation of the two poles of the bipole about each other as the bipole emerges. The eruption comes from inside the emerging bipole and blows it open to produce a CME observed by SOHO/LASCO. That eruption is preceded by flux cancellation at the emerging bipole's interior PIL, cancellation that plausibly builds a sheared and twisted flux rope above the interior PIL and fnally triggers the blow-out eruption of the flux rope via photospheric-convection-driven, slow tether-cutting reconnection of the legs of the sheared core field, low above the interior PIL, as proposed by van Ballegooijen and Martens (1989, ApJ, 343, 971) and Moore and Roumeliotis (1992, in Eruptive Solar Flares, ed. Z. Svestka, B.V. Jackson, and M.E. Machado [Berlin:Springer], 69). The production of this eruption is a (perhaps rare) counterexample to solar eruptions that result from external collisional shearing between opposite polarities from two distinct emerging and/or emerged bipoles (Chintzoglou et al., 2019, ApJ, 871:67).

Adams, Mitzi L.↗

Fiber-Optic Strain-Based Deflection and Twist Sensing for a High-Aspect-Ratio Swept Wing

Designs of aircraft structures have been moving toward leaner, lightweight designs for increased fuel efficiency. The Passive Aeroelastic Tailored (PAT) wing developed under the NASA Advanced Air Transport Technology (AATT) project is an example of a swept-wing design with high aspect ratio that incorporates lightweight highly-flexible tailored composite construction. The passive aeroelastic tailored structural design has explored the design space to enable aeroelastically tailored wing structures to increase aspect ratios (from 9 to 14) and ultimately reduce weight by 20 percent to 25 percent without impacting aeroelastic performance. To further study the aeroelastic performance of such a wing, the NASA Armstrong Flight Research Center (AFRC) (Edwards, California) has developed efficient real-time structural algorithms that are used in conjunction with a fiber-optic measurement system for lightweight vehicle applications. The AFRC Fiber Optic Sensing System (FOSS) provides up to 8,000 distributed surface strain measurements at one-half-inch increments and can be used to estimate a variety of structural parameters such as shape and load. This report discusses the implementation of strain-based displacement and twist-sensing techniques applied to the PAT wing test article tested at the NASA AFRC Flight Loads Laboratory. Empirical FOSS strain data are collected under varying loading conditions. Strain data are processed with the displacement and twist-sensing algorithms and independently verified by comparison to conventional ground-based instrumentation.

Pena, Francisco↗

Acoustics and Performance Characteristics of an Ideally Twisted Rotor in Hover

A series of experiments were conducted in an anechoic chamber to investigate the noise and performance of an ideally twisted rotor design, leading to validation of a low-fidelity aerodynamic performance and acoustic modeling tool chain. An "ideally twisted" rotor was designed in order to simplify the theoretical rotor inflow for a target thrust condition in hover. This rotor design was then fabricated using state-of-the-art rapid prototyping and tested in an anechoic chamber facility. Aerodynamic load and acoustic data were acquired across a range of rotation rates (RPM) and rotor collective settings in order to ascertain the accuracy of the low-fidelity modeling codes. Emphasis was placed on modeling of the broadband self-noise generated by the rotor system due to the fact that it was found to be a prominent contributor to the overall rotor system noise.

Nicole A. Pettingill↗

Acoustic and Performance Characteristics of an Ideally Twisted Rotor in Hover

A series of experiments were conducted in an anechoic chamber to investigate the noise and performance of an ideally twisted rotor design, leading to validation of a low-fidelity aerodynamic performance and acoustic modeling tool chain. An “ideally twisted” rotor was designed in order to simplify the theoretical rotor inflow for a target thrust condition in hover. This rotor design was then fabricated using state-of-the-art rapid prototyping and tested in an anechoic chamber. Aerodynamic load and acoustic data were acquired across a range of rotation rates and rotor collective settings in order to both identify noise trends and ascertain the accuracy of the low-fidelity modeling codes. Emphasis was placed on modeling of the broadband self-noise generated by the rotor system due to the fact that it was found to be a prominent contributor to the overall rotor system noise.

Nicole A Pettingill↗

Preliminary Measurements of Partial Discharge Inception Voltage Degradation with Constant Temperature Aging of Magnet Wire Twisted Pairs for Electric Aircraft Motors

Electric aircraft require high reliability electric motors to meet the stringent safety standards of the aviation industry. Motor winding insulation lifetime and reliability models for electric aircraft applications are needed to enable design of high reliability and high-performance electric motors for aviation applications. Three primary stresses effect the lifetime of motor winding insulation: thermochemical, mechanical, and electrical. This paper presents preliminary experiments on twisted pairs of magnet wire targeting understanding the thermo-chemical degradation of motor winding insulation through constant temperature thermal aging. Three types of magnet wire twisted pairs where tested: polyimide insulated magnet wire, epoxy coated polyimide insulated magnet wire, and epoxy potted polyimide magnet wire. The degradation of the samples over time at temperature was tracked using partial discharge inception voltage measurements. These initial experiments were used for development of procedures and expertise in partial discharge testing and thermo-chemical aging to inform future testing. The preliminary nature of these experiments should be noted when viewing the results. Lessons learned from the experiments are documented.

Thomas Tallerico↗

Low-speed static longitudinal and lateral stability characteristics of two low-aspect-ratio wings cambered and twisted to provide a uniform load at a supersonic flight condition

A delta wing and a tapered sweptback wing of aspect ratios 1.56 and 2.00, respectively, both of which were cambered and twisted so as to provide a uniform load distribution for a supersonic flight condition, were tested in combination with a fuselage at Reynolds numbers between 384,000 and 1,550,000 in order to determine the low-speed lift-drag and static stability characteristics of such wings.

REYNOLDS NUMBER EFFECTS - COMPLETE WINGS↗