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DOE OSTI2025

De novo synthesis and near atomic resolution imaging of host immune receptors critical for pathogen recognition (Abbreviated Final Report)

The innate immune system serves as the body’s first line of defense against invading pathogens, responding rapidly through the deployment of immune cells at common sites of infection, such as the skin and airways. These immune-cell sentinels express a range of highly conserved receptors, including Toll-like receptors (TLRs), which recognize and bind to pathogen-derived components. This recognition event triggers intracellular signaling cascades that initiate and coordinate immune responses. Despite their significance, the complete structural characterization of full-length TLRs, including their extracellular domain (ECD), transmembrane domain (TMD), and Toll/interleukin-1 receptor (TIR) domain, remains incomplete. In this study, we examined the expression and isolation of human TLR4 incorporated into nanodiscs using two approaches: a cell-free synthesis system and a cell-based transfection strategy employing Expi293F cells derived from the human embryonic kidney lineage. Our findings demonstrate that NLP-bound human TLR4 produced via the cell-based method yielded functional protein suitable for time-resolved single-particle cryo-electron microscopy (cryo-EM). This advancement enables structural characterization of full-length TLR4, maintaining the integrity of its extracellular domain, transmembrane domain, and Toll/Interleukin-1 receptor (TIR) domain.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2022

Environmental Effects in Herschel Observations of the Ionized Carbon Content of Star-forming Dwarf Galaxies in the Virgo Cluster

We use archival Herschel data to examine the singly ionized carbon ([C II]) content of 14 star-forming dwarf galaxies in the Virgo cluster. We use spectral energy distribution fits to far-infrared, mid-infrared, near-infrared, optical, and ultraviolet data to derive the total infrared continuum (TIR) for these galaxies. We compare the [C II]/TIR ratio for dwarf galaxies in the central part of Virgo to those in the southern part of the cluster and to galaxies with similar TIR luminosities and metallicities in the Herschel Dwarf Galaxy Survey (DGS) sample of field dwarf galaxies to look for signs of [C II] formation independent of star formation. Our analysis indicates that the sample of Virgo dwarfs in the central part of the cluster has significantly higher values of [C II]/TIR than the sample from the southern part of the cluster and the sample from the DGS, while the southern sample is consistent with the DGS. This [C II]/TIR excess implies that a significant fraction of the [C II] in the dwarf galaxies in the cluster center has an origin other than star formation and is likely to be due to environmental processes in the central part of the cluster. We also find a surprisingly strong correlation between [C II]/TIR and the local ram pressure felt by the dwarf galaxies in the cluster. In this respect, we claim that the excess [C II] we see in these galaxies is likely to be due to formation in ram-pressure shocks.

79 ASTRONOMY AND ASTROPHYSICS↗

DOE OSTI2020

A Multi-Sensor Unoccupied Aerial System Improves Characterization of Vegetation Composition and Canopy Properties in the Arctic Tundra: Supporting Data

Remote sensing data collected from Brookhaven National Laboratory's (BNL) heavy-lift unoccupied aerial system (UAS) octocopter platform - the Osprey - operated by the Terrestrial Ecosystem Science and Technology (TEST) group. Data was collected from a single flight over the Kougarok hillslope site on 26 July, 2018. The Osprey is a multi-sensor UAS platform that simultaneously measures very high spatial resolution optical red/green/blue (RGB) and thermal infrared (TIR) surface "skin" temperature imagery, as well as surface reflectance at 1nm intervals in the visible to near-infrared spectral range from ~350-1000 nm measured at regular intervals along each flight path. Derived image products include ortho-mosaiced RGB and TIR images, an RGB-based digital surface model (DSM) using the structure from motion (SfM) technique, digital terrain model (DTM), and a canopy height model. Ancillary aircraft data, flight mission parameters, and general flight conditions are also included. This dataset includes *.pdf, *.txt, *.tif, *.dat, and *.csv with most zipped in *.tar.gz files. The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), was a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy's Office of Biological and Environmental Research. The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska. Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2021

Landscape-scale Characterization of Arctic Tundra Vegetation Composition, Structure, and Function with a Multi-sensor Unoccupied Aerial System: Supporting Data.

Airborne remote sensing data collected using the Brookhaven National Laboratory's (BNL) heavy-lift unoccupied aerial system (UAS) octocopter platform, the Osprey, operated by the Terrestrial Ecosystem Science and Technology (TEST) group (https://www.bnl.gov/testgroup). This package includes data from three flights flown over the NGEE-Arctic Council, Kougarok and Teller sites in July, 2018. The Osprey is a multi-sensor UAS platform that simultaneously measures very high spatial resolution optical red/green/blue (RGB) and thermal infrared (TIR) surface 'skin' temperature imagery, as well as surface reflectance at 1 nm intervals in the visible to near-infrared spectral range 350 - 1000 nm measured at regular intervals along each flight path. Derived image products include ortho-mosaiced RGB and TIR images, an RGB-based digital surface model (DSM) using the structure from motion (SfM) technique, digital terrain model (DTM), and a canopy height model (CHM). In addition, a VNIR surface reflectance file is provided for the trigger locations collected during each flight campaign. Ancillary aircraft data, flight mission parameters, and general flight conditions provided by the onboard flight and data collection computers are also included. Unprocessed and processed data products are included in this package (processing levels 0-3). Data and metadata are provided as text (*.txt, *.json, *.kml, *hdr, *.enp), tabular (*.dat, *.csv, *.waypoint, ENVI format (no extension)), point cloud (*.laz) and image (*.jpg, *.tif, *png) formats. This metadata document contains flight campaign, instrument and file metadata, along with a description of data processing levels, data products and file naming scheme.The Next-Generation Ecosystem Experiments: Arctic (NGEE Arctic), a research effort to reduce uncertainty in Earth System Models by developing a predictive understanding of carbon-rich Arctic ecosystems and feedbacks to climate. NGEE Arctic was supported by the Department of Energy?s Office of Biological and Environmental Research.The NGEE Arctic project had two field research sites: 1) located within the Arctic polygonal tundra coastal region on the Barrow Environmental Observatory (BEO) and the North Slope near Utqiagvik (Barrow), Alaska and 2) multiple areas on the discontinuous permafrost region of the Seward Peninsula north of Nome, Alaska.Through observations, experiments, and synthesis with existing datasets, NGEE Arctic provided an enhanced knowledge base for multi-scale modeling and contributed to improved process representation at global pan-Arctic scales within the Department of Energy's Earth system Model (the Energy Exascale Earth System Model, or E3SM), and specifically within the E3SM Land Model component (ELM).

54 ENVIRONMENTAL SCIENCES↗

NASA NTRS1983

Advanced microwave soil moisture studies

Comparisons of low level L-band brightness temperature (TB) and thermal infrared (TIR) data as well as the following data sets: soil map and land cover data; direct soil moisture measurement; and a computer generated contour map were statistically evaluated using regression analysis and linear discriminant analysis. Regression analysis of footprint data shows that statistical groupings of ground variables (soil features and land cover) hold promise for qualitative assessment of soil moisture and for reducing variance within the sampling space. Dry conditions appear to be more conductive to producing meaningful statistics than wet conditions. Regression analysis using field averaged TB and TIR data did not approach the higher sq R values obtained using within-field variations. The linear discriminant analysis indicates some capacity to distinguish categories with the results being somewhat better on a field basis than a footprint basis.

Dalsted, K. J.↗

NASA NTRS1984

Testing the radiometric stability of HCMM thermal infrared data

A study conducted to test the radiometric stability of thermal infrared (TIR) data from the heat-capacity mapping mission (HCMM) satellites is considered. The radiance values associated with various land use and cover types in a regional study area centered on Washington, D.C. are examined. The study shows that for three different day TIR-data sets, the relative ranking of mean thermal values associated with five Level I and three Level II land-use/land-cover categories remains constant over time. Although HCMM predicted temperatures show variability up to 5 C from ground observed temperatures, the thermal measurements recorded by the satellite are fairly stable as indicators of surface temperature. A method for combining HCMM thermal data and Landsat multispectral scanner (MSS) data to improve the classification of Level I land-cover categories, and in particular the separability of urban and nonurban areas is described. A merged HCMM-MSS data set is found to yield the best results in terms of thematic-map accuracy.

Witt, R. G.↗

NASA NTRS1988

The basis for the spectral behaviour of silicates in the thermal infrared and applications to remote sensing

Variations in the thermal infrared (TIR) spectral response of silicate rocks is related to changes in the structures and divalent cation contents of the minerals which form the rocks. These considerations lead to a chemical parameter, SCFM, which reflects mineral structures, rock types, and their spectra. The parameter is the ratio of silica to the abundance of depolymerizing cations, defined as SCFM = SiO2/SiO2 + CaO + MgO + FeO. Parameter SCFM is therefore proposed for use in TIR remote sensing of igneous rocks. It is also demonstrated that two or three broad bands are sufficient for distinguishing among major rock types and the system noise has little effect on the quality of the results. These factors can be traded off against improved spatial resolution in instrument design.

Walter, L. S.↗

NASA NTRS1992

Enhancement of multispectral thermal infrared images - Decorrelation contrast stretching

Decorrelation contrast stretching is an effective method for displaying information from multispectral thermal infrared (TIR) images. The technique involves transformation of the data to principle components ('decorrelation'), independent contrast 'stretching' of data from the new 'decorrelated' image bands, and retransformation of the stretched data back to the approximate original axes, based on the inverse of the principle component rotation. The enhancement is robust in that colors of the same scene components are similar in enhanced images of similar scenes, or the same scene imaged at different times. Decorrelation contrast stretching is reviewed in the context of other enhancements applied to TIR images.

Gillespie, Alan R.↗

NASA NTRS1994

Land surface temperature measurements for EOS MODIS data

Work accomplished includes: Beta delivery 1 of the MODIS LST product; the first version of MODIS LST ATBD; update of the atmospheric radiative transfer code ATRAD; the development of a new approach look-up table method; and improvement of the TIR spectrometer. Preliminary feasibility analysis of the look-up table approach is presented in terms of showing the effects on the TIR radiance at the top of the atmosphere of the stratospheric and upper atmospheric temperature profiles, the surface emissivity and temperature, the lower atmospheric temperature and water vapor profiles, and the viewing angle.

Wan, Zhengming↗

NASA NTRS1993

AVIRIS and TIMS data processing and distribution at the land processes distributed active archive center

The U.S. Government has initiated the Global Change Research program, a systematic study of the Earth as a complete system. NASA's contribution of the Global Change Research Program is the Earth Observing System (EOS), a series of orbital sensor platforms and an associated data processing and distribution system. The EOS Data and Information System (EOSDIS) is the archiving, production, and distribution system for data collected by the EOS space segment and uses a multilayer architecture for processing, archiving, and distributing EOS data. The first layer consists of the spacecraft ground stations and processing facilities that receive the raw data from the orbiting platforms and then separate the data by individual sensors. The second layer consists of Distributed Active Archive Centers (DAAC) that process, distribute, and archive the sensor data. The third layer consists of a user science processing network. The EOSDIS is being developed in a phased implementation. The initial phase, Version 0, is a prototype of the operational system. Version 0 activities are based upon existing systems and are designed to provide an EOSDIS-like capability for information management and distribution. An important science support task is the creation of simulated data sets for EOS instruments from precursor aircraft or satellite data. The Land Processes DAAC, at the EROS Data Center (EDC), is responsible for archiving and processing EOS precursor data from airborne instruments such as the Thermal Infrared Multispectral Scanner (TIMS), the Thematic Mapper Simulator (TMS), and Airborne Visible and Infrared Imaging Spectrometer (AVIRIS). AVIRIS, TIMS, and TMS are flown by the NASA-Ames Research Center ARC) on an ER-2. The ER-2 flies at 65000 feet and can carry up to three sensors simultaneously. Most jointly collected data sets are somewhat boresighted and roughly registered. The instrument data are being used to construct data sets that simulate the spectral and spatial characteristics of the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) instrument scheduled to be flown on the first EOS-AM spacecraft. The ASTER is designed to acquire 14 channels of land science data in the visible and near-IR (VNIR), shortwave-IR (SWIR), and thermal-IR (TIR) regions from 0.52 micron to 11.65 micron at high spatial resolutions of 15 m to 90 m. Stereo data will also be acquired in the VNIR region in a single band. The AVIRIS and TMS cover the ASTER VNIR and SWIR bands, and the TIMS covers the TIR bands. Simulated ASTER data sets have been generated over Death Valley, California, Cuprite, Nevada, and the Drum Mountains, Utah using a combination of AVIRIS, TIMS, amd TMS data, and existing digital elevation models (DEM) for the topographic information.

Mah, G. R.↗

NASA NTRS1993

Mineralogic variability of the Kelso Dunes, Mojave Desert, California derived from Thermal Infrared Multispectral Scanner (TIMS) data

Mineral identification and mapping of alluvial material using thermal infrared (TIR) remote sensing is extremely useful for tracking sediment transport, assessing the degree of weathering and locating sediment sources. As a result of the linear relation between a mineral's percentage in a given area (image pixel) and the depth of its diagnostic spectral features, TIR spectra can be deconvolved in order to ascertain mineralogic percentages. Typical complications such as vegetation, particle size and thermal shadowing are minimized upon examination of dunes. Actively saltating dunes contain little to no vegetation, are very well sorted and lack the thermal shadows that arise from rocky terrain. The primary focus of this work was to use the Kelso Dunes as a test location for an accuracy analysis of temperature/emissivity separation and linear unmixing algorithms. Accurate determination of ground temperature and component discrimination will become key products of future ASTER data. A decorrelation stretch of the TIMS image showed clear color variations within the active dunes. Samples collected from these color units were analyzed for mineralogy, grain size, and separated into endmembers. This analysis not only revealed that the dunes contained significant mineralogic variation, but were more immature (low quartz percentage) than previously reported. Unmixing of the TIMS data using the primary mineral endmembers produced unique variations within the dunes and may indicate near, rather than far, source locales for the dunes. The Kelso Dunes lie in the eastern Mojave Desert, California, approximately 95 km west of the Colorado River. The primary dune field is contained within a topographic basin bounded by the Providence, Granite Mountains, with the active region marked by three northeast trending linear ridges. Although active, the dunes appear to lie at an opposing regional wind boundary which produces little net movement of the crests. Previous studies have estimated the dunes range from 70% to 90% quartz mainly derived from a source 40 km to the west. The dune field is assumed to have formed in a much more arid climate than present, with the age of the deposit estimated at greater than 100,000 years.

Ramsey, Michael S.↗

NASA NTRS1993

Land surface temperature measurements from EOS MODIS data

The task objectives of this reporting phase included: (1) completing the draft of the LST Algorithms Theoretical Basic Document by July 30, 1993; (2) making a detailed characterization of the thermal infrared measurement system including spectrometer, blackbody, and radiation sources; (3) making TIR spectral measurements of water and snow-cover surfaces with the MIDAC M2401 spectrometer; and (4) making conceptual and engineering design of an accessory system for spectrometric measurements at variable angles. These objectives are based on the requirements by the MODIS Science Team and the unique challenge in the development of MODIS LST algorithms: to acquire accurate spectral emissivity data of land covers in the near-term and to make ground validations of the LST product in the long-term with a TIR measurement system.

Wan, Zhengming↗

NASA NTRS1998

Land Surface Temperature Measurements from EOD MODIS Data

We made more tests of the version 2.0 daily Level 2 and Level 3 Land-Surface Temperature (LST) code (PGE 16) jointly with the MODIS Science Data Support Team (SDST). After making minor changes a few times, the PGE16 code has been successfully integrated and tested by MODIS SDST, and recently has passed the inspection at the Goddard Distributed Active Archive Center (DAAC). We conducted a field campaign in the area of Mono Lake, California on March 10, 1998, in order to validate the MODIS LST algorithm in cold and dry conditions. Two MODIS Airborne Simulator (MAS) flights were completed during the field campaign, one before noon, and another around 10 pm PST. The weather condition for the daytime flight was perfect: clear sky, the column water vapor measured by radiosonde around 0.3 cm, and wind speed less than a half meter per second. The quality of MAS data is good for both day and night flights. We analyzed the noise equivalent temperature difference (NE(delta)T) and the calibration accuracy of the seven MAS thermal infrared (TIR) bands, that are used in the MODIS day/night LST algorithm, with daytime MAS data over four flat homogeneous study areas: two on Grant Lake (covered with ice and snow, respectively), one on Mono Lake, and another on the snow field site where we made field measurements. NE(delta)T ranges from 0.2 to 0.6 k for bands 42, 45, 46, and 48. It ranges from 0.8 to 1.1 K for bands 30-32. The day and night MAS data have been used to retrieve surface temperature and emissivities in these bands. A simple method to correct the effect of night thin cirrus has been incorporated into the day/night LST algorithm in dry atmospheric conditions. We compared the retrieved surface temperatures with those measured with TIR spectrometer, radiometers and thermistors in the snow test site, and the retrieved emissivity images with topographic map. The daytime LST values match well within 1 K. The night LST retrieved from MAS data is 3.3 K colder than those from field measurements most likely because of the effect of haze at night. The good agreement among the regional averaged surface temperatures obtained from LST values retrieved at different resolutions increased our confidence in the MODIS day/night LST algorithm.

Wan, Zheng-Ming↗

NASA NTRS2000

CPL Applications on the EOS-TERRA Spacecraft

This presentation reviews the use of Capillary Pumped Loops on the EOS-TERRA spacecraft, It starts with a brief review of the status of CPL technology. On the TERRA spacecraft, CPL's will be used for thermal control of 3 instruments. The document reviews the ground testing, and states that life testing will continue to run for three year, even after the launch.The document has schematic diagrams of the EOS-AM spacecraft, the Advanced Spaceborne Thermal Emission and Reflection Radiometer - Thermal-Infrared Radiometer (ASTER-TIR) CPL configuration, the Advanced Spaceborne Thermal Emission and Reflection Radiometer - Short-Wave-Infrared Radiometer (ASTER-SWIR). The use of Measurements of Pollution In The Troposphere (MOPITTS) Capillary Pumped Heat Transport System (CPHTS) is reviewed, and the performance is summarized in several charts. The use of CPHTS in the ASTER-SWIR is reviewed. The loops in the ASTER-TIR module is also reviewed, and the problems with the second loop temperature control is discussed.

Ku, Jentung↗

NASA NTRS1999

Exploring the Effects of Dust Coatings and Shock Pressures on Thermal Infrared Spectra

Dust coatings on rocks obscured the underlying rock surfaces at the Mars Pathfinder landing site and made it difficult to obtain pristine visible/near-infrared reflectance spectra and chemical measurements. Such dust coatings also affect the ability of thermal infrared (TIR) spectra (such as that provided by TES and future lander (mini-TES) and orbiter multispectral imaging systems (THEMIS) to reveal accurately the mineralogy of rocks and soils. Similarly, high shock pressures from impact events cause crystalline disorder in important minerals such as pyroxene and feldspar that also affects their TIR spectra and complicates spectral interpretation of shocked impact materials. Additional information is contained in the original extended abstract.

Johnson, J. R.↗

NASA NTRS2004

Near Space Tracking of the EM Phenomena Associated with the Main Earthquakes

Searching for electromagnetic (EM) phenomena originating in the Earth's crust prior to major earthquakes (M>5) are the object of this exploratory study. We present the idea of a possible relationship between: (1) electro-chemical and thermodynamic processes in the Earth's crust and (2) ionic enhancement of the atmosphere/ionosphere with tectonic stress and earthquake activity. The major source of these signals are proposed to originate from electromagnetic phenomenon which are responsible for these observed pre-seismic processes, such as, enhanced IR emission, also born as thermal anomalies, generation of long wave radiation, light emission caused by ground-to-air electric discharges, Total Electron Content (TEC) ionospheric anomalies and ionospheric plasma variations. The source of these data will include: (i) ionospheric plasma perturbations data from the recently launched DEMETER mission and currently available TEC/GPS network data; (ii) geomagnetic data from ORSTED and CHAMP; (iii) Thermal infra-red (TIR) transients mapped by the polar orbiting (NOAA/AVHRR, MODIS) and (iv) geosynchronous weather satellites measurements of GOES, METEOSAT. This approach requires continues observations and data collecting, in addition to both ground and space based monitoring over selected regions in order to investigate the various techniques for recording possible anomalies. During the space campaign emphasis will be on IR emission, obtained from TIR (thermal infrared) satellites, that records land/sea surface temperature anomalies and changes in the plasma and total electron content (TEC) of the ionosphere that occur over areas of potential earthquake activity.

Ouzounov, Dimitar↗

NASA NTRS2004

Progress in Understanding the Pre-Earthquake Associated Events by Analyzing IR Satellite Data

We present latest result in understanding the potential relationship between tectonic stress, electro-chemical and thermodynamic processes in the Earths crust and atmosphere with an increase in IR flux as a potential signature of electromagnetic (EM) phenomena that are related to earthquake activity, either pre-, co- or post seismic. Thermal infra-red (TIR) surveys performed by the polar orbiting (NOAA/AVHRR MODIS) and geosynchronous weather satellites (GOES, METEOSAT) gave an indication of the appearance (from days to weeks before the event) of "anomalous" space-time TIR transients that are associated with the location (epicenter and local tectonic structures) and time of a number of major earthquakes with M>5 and focal depths less than 50km. We analyzed broad category of associated pre-earthquake events, which provided evidence for changes in surface temperature, surface latent heat flux, chlorophyll concentrations, soil moisture, brightness temperature, emissivity of surface, water vapour in the atmosphere prior to the earthquakes occurred in Algeria, India, Iran, Italy, Mexico and Japan. The cause of such anomalies has been mainly related to the change of near-surface thermal properties due to complex lithosphere-hydrosphere-atmospheric interactions. As final results we present examples from the most recent (2000-2004) worldwide strong earthquakes and the techniques used to capture the tracks of EM emission mid-IR anomalies and a methodology for practical future use of such phenomena in the early warning systems.

Ouzounov, Dimitar↗

NASA NTRS2005

Mapping Weathering and Alteration Minerals in the Comstock and Geiger Grade Areas using Visible to Thermal Infrared Airborne Remote Sensing Data

To support research into both precious metal exploration and environmental site characterization a combination of high spatial/spectral resolution airborne visible, near infrared, short wave infrared (VNIR/SWIR) and thermal infrared (TIR) image data were acquired to remotely map hydrothermal alteration minerals around the Geiger Grade and Comstock alteration regions, and map the mineral by-products of weathered mine dumps in Virginia City. Remote sensing data from the Airborne Visible Infrared Imaging Spectrometer (AVIRIS), SpecTIR Corporation's airborne hyperspectral imager (HyperSpecTIR), the MODIS-ASTER airborne simulator (MASTER), and the Spatially Enhanced Broadband Array Spectrograph System (SEBASS) were acquired and processed into mineral maps based on the unique spectral signatures of image pixels. VNIR/SWIR and TIR field spectrometer data were collected for both calibration and validation of the remote data sets, and field sampling, laboratory spectral analyses and XRD analyses were made to corroborate the surface mineralogy identified by spectroscopy. The resulting mineral maps show the spatial distribution of several important alteration minerals around each study area including alunite, quartz, pyrophyllite, kaolinite, montmorillonite/muscovite, and chlorite. In the Comstock region the mineral maps show acid-sulfate alteration, widespread propylitic alteration and extensive faulting that offsets the acid-sulfate areas, in contrast to the larger, dominantly acid-sulfate alteration exposed along Geiger Grade. Also, different mineral zones within the intense acid-sulfate areas were mapped. In the Virginia City historic mining district the important weathering minerals mapped include hematite, goethite, jarosite and hydrous sulfate minerals (hexahydrite, alunogen and gypsum) located on mine dumps. Sulfate minerals indicate acidic water forming in the mine dump environment. While there is not an immediate threat to the community, there are clearly sources of acidic drainage that were identified remotely.

imaging spectroscopy↗