Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “SPLICE”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 145 records · Page 8

The characterization of widespread fatigue damage in fuselage structure

The characteristics of widespread fatigue damage (WSFD) in fuselage riveted structure were established by detailed nondestructive and destructive examinations of fatigue damage contained in a full size fuselage test article. The objectives of this were to establish an experimental data base for validating emerging WSFD analytical prediction methodology and to identify first order effects that contribute to fatigue crack initiation and growth. Detailed examinations were performed on a test panel containing four bays of a riveted lap splice joint. The panel was removed from a full scale fuselage test article after receiving 60,000 full pressurization cycles. The results of in situ examinations document the progression of fuselage skin fatigue crack growth through crack linkup. Detailed tear down examinations and fractography of the lap splice joint region revealed fatigue crack initiation sites, crack morphology, and crack linkup geometry. From this large data base, distributions of crack size and locations are presented and discussions of operative damage mechanisms are offered.

Piascik, Robert S.↗

The characterization of widespread fatigue damage in fuselage structure

The characteristics of widespread fatigue damage (WSFD) in fuselage riveted structure were established by detailed nondestructive and destructive examinations of fatigue damage contained in a full size fuselage test article. The objectives of this work were to establish an experimental data base for validating emerging WSFD analytical prediction methodology and to identify first order effects that contribute to fatigue crack initiation and growth. Detailed examinations were performed on a test panel containing four bays of a riveted lap splice joint. The panel was removed from a full scale fuselage test article after receiving 60,000 full pressurization cycles. The results of in situ examinations document the progression of fuselage skin fatigue crack growth through crack linkup. Detailed tear down examinations and fractography of the lap splice joint region revealed fatigue crack initiation sites, crack morphology and crack linkup geometry. From this large data base, distributions of crack size and locations are presented and discussions of operative damage mechanisms are offered.

Piascik, Robert S.↗

Hoop Tensile Characterization Of SiC/SiC Cylinders Fabricated From 2D Fabric

Tensile stress-strain properties in the hoop direction were obtained for 100-mm diameter SiC/SiC cylinders using ring specimens machined from the cylinder ends. The cylinders were fabricated from 2D balanced fabric with several material variants, including wall thickness (6, 8, and 12 plies), Sic fiber type (Sylramic, Sylramic-iBN, Hi-Nicalon, and Hi-Nicalon S), fiber sizing type, and matrix type (full CVI Sic, and partial CVI plus melt-infiltrated SiC-Si). Fiber ply splices existed in the all the hoops. Tensile hoop measurements were made at room temperature and 1200 C using hydrostatic ring test facilities. The hoop results are compared with in-plane data measured on flat panels using same material variants, but containing no splices.

Verrilli, Michael J.↗

Left-handed Z-DNA: structure and function

Z-DNA is a high energy conformer of B-DNA that forms in vivo during transcription as a result of torsional strain generated by a moving polymerase. An understanding of the biological role of Z-DNA has advanced with the discovery that the RNA editing enzyme double-stranded RNA adenosine deaminase type I (ADAR1) has motifs specific for the Z-DNA conformation. Editing by ADAR1 requires a double-stranded RNA substrate. In the cases known, the substrate is formed by folding an intron back onto the exon that is targeted for modification. The use of introns to direct processing of exons requires that editing occurs before splicing. Recognition of Z-DNA by ADAR1 may allow editing of nascent transcripts to be initiated immediately after transcription, ensuring that editing and splicing are performed in the correct sequence. Structural characterization of the Z-DNA binding domain indicates that it belongs to the winged helix-turn-helix class of proteins and is similar to the globular domain of histone-H5.

Review↗

Hoop Tensile Properties of Ceramic Matrix Composite Cylinders

Tensile stress-strain properties in the hoop direction were obtained for 100-mm diameter SiC/SiC ceramic matrix composite cylinders using ring specimens machined form the cylinder ends. The cylinders were fabricated from 2D balanced SiC fabric with several material variants, including wall thickness (6,8, and 12 plies), SiC fiber type (Sylramic, Sylramic-iBN, Hi-Nicalon, and Hi-Nicalon S), fiber sizing type, and matrix type (full CVI SiC, and partial CVI SiC plus slurry cast + melt-infiltrated SiC-Si). Fiber ply splices existed in all the hoops. Tensile hoop measurements are made at room temperature and 1200 C using hydrostatic ring test facilities. The failure mode of the hoops, determined through microstructural examination, is presented. The hoop properties are compared with in-plane data measured on flat panels using same material variants, but containing no splices.

Verrilli, Michael J.↗

SiC Composite Turbine Vanes

Turbine inlet guide vanes have been fabricated from composites of silicon carbide fibers in silicon carbide matrices. A unique design for a cloth made from SiC fibers makes it possible to realize the geometric features necessary to form these vanes in the same airfoil shapes as those of prior metal vanes. The fiber component of each of these vanes was made from SiC-fiber cloth coated with boron nitride. The matrix was formed by chemical-vapor infiltration with SiC, then slurry-casting of SiC, followed by melt infiltration with silicon. These SiC/SiC vanes were found to be capable of withstanding temperatures 400 F (222 C) greater than those that can be withstood by nickel-base-superalloy turbine airfoils now in common use in gas turbine engines. The higher temperature capability of SiC/SiC parts is expected to make it possible to use them with significantly less cooling than is used for metallic parts, thereby enabling engines to operate more efficiently while emitting smaller amounts of NOx and CO. The SiC/SiC composite vanes were fabricated in two different configurations. Each vane of one of the configurations has two internal cavities formed by a web between the suction and the pressure sides of the vane. Each vane of the other configuration has no web (see Figure 1). It is difficult to fabricate components having small radii, like those of the trailing edges of these vanes, by use of stiff stoichiometric SiC fibers currently preferred for SiC/SiC composites. To satisfy the severe geometric and structural requirements for these vanes, the aforementioned unique cloth design, denoted by the term Y-cloth, was conceived (see Figure 2). In the regions away from the trailing edge, the Y-cloth features a fiber architecture that had been well characterized and successfully demonstrated in combustor liners. To form a sharp trailing edge (having a radius of 0.3 mm), the cloth was split into two planes during the weaving process. The fiber tows forming the trailing-edge section were interlocked, thereby enhancing through-thickness strength of the resulting composite material. For vanes of the webless configuration, each made from a layup of six plies of Ycloth, the length of each Y-cloth layer was cut so that the two strips corresponding to the aforementioned two planes would wrap around the perimeter of a graphite vane preform tool with a 10-mm overlap. The overlap was used to join the two strips in a fringe splice. To make the external sixth ply, a standard woven cloth was cut to the required final length and a fringe splice joined the two ends of the cloth at the trailing edge. The cloth was then prepregged. The entire assembly was then placed into an aluminum compaction tool designed to form the outer net shape of the vane. After the prepreg material was allowed to dry, the preform was removed from the aluminum tooling and placed into an external graphite tool before being shipped to a vendor for matrix infiltration. To make the SiC fiber preform for a vane having an internal web, a slightly different initial approach was followed. Each of two sections forming the internal cavities (and ultimately the web) was created by first slipping two concentric layers of a two-dimensional, 2-by-2, 45 - braided tube around a net-shape graphite mandrel. The tubes on both mandrels were prepregged and allowed to dry. The resulting two subassemblies were put together, then four additional plies were wrapped around them in the same fashion as that described above for the six plies of the vaneless configuration. The consolidation of the SiC fiber preforms into SiC/SiC composite parts was performed by commercial vendors using their standard processes. The capability of two of the webless SiC/SiC turbine vanes was demonstrated in tests in a turbine environment. The tests included 50 hours of steady-state operation and 102 two-minute thermal cycles. A surface temperature of 1,320 C was reached during the tests.

Calomino, Anthony M.↗

Development of a Climatology of Vertically Complete Wind Profiles from Doppler Radar Wind Profiler Systems

Assessment of space vehicle loads and trajectories during design requires a large sample of wind profiles at the altitudes where winds affect the vehicle. Traditionally, this altitude region extends from near 8-14 km to address maximum dynamic pressure upon ascent into space, but some applications require knowledge of measured wind profiles at lower altitudes. Such applications include crew capsule pad abort and plume damage analyses. Two Doppler Radar Wind Profiler (DRWP) systems exist at the United States Air Force (USAF) Eastern Range and at the National Aeronautics and Space Administration's Kennedy Space Center. The 50-MHz DRWP provides wind profiles every 3-5 minutes from roughly 2.5-18.5 km, and five 915-MHz DRWPs provide wind profiles every 15 minutes from approximately 0.2-3.0 km. Archived wind profiles from all systems underwent rigorous quality control (QC) processes, and concurrent measurements from the QC'ed 50- and 915-MHz DRWP archives were spliced into individual profiles that extend from about 0.2-18.5 km. The archive contains combined profiles from April 2000 to December 2009, and thousands of profiles during each month are available for use by the launch vehicle community. This paper presents the details of the QC and splice methodology, as well as some attributes of the archive.

Barbre, Robert, Jr.↗

A Simulation Framework for Precision Landing and Hazard Avoidance Technology Assessments

To meet NASA’s challenge to return humans to the Moon in 2024 and establish a sustainable presence in 2028 requires advances in autonomous spacecraft navigation. The Safe and Precise Landing Integrated Capabilities Evolution (SPLICE) project, which leverages previous work at NASA to develop multi-mission precision landing and hazard avoidance technologies, is using a multi-faceted approach to achieve the advanced landing requirements. In addition to increasing the technology readiness level of key sensors and developing high performance space computing, SPLICE uses simulations to determine navigation requirements and evaluate sensor performance. The effort evaluates various precision landing concepts of operations, not only for the lunar human and robotic missions, but also for potential missions to other solar system destinations. This paper summarizes the six degree-of-freedom high fidelity simulation framework, trajectory design methodology, and sensor models being considered for a variety of precision lander missions. Initial results of the navigation sensor performance for a human Mars mission are presented. Finally, trade and sensitivity studies are outlined for future work to fully characterize sensor performance assumptions and modifications required to achieve precision landing and hazard avoidance.

Alicia Dwyer Cianciolo↗

NOR Flash Memory Scrubbing Application for Boot File Preservation of NASA’s Descent and Landing Computer (DLC)

Progress on NASA’s Safe and Precise Landing Integrated Capabilities Evolution (SPLICE) project continues, specifically with this development of the Descent and Landing Computer (DLC). One of the DLC’s primary contributions as a SPLICE technology is its implementation of algorithms and operation of sensors to autonomously guide a spacecraft in performing more precise and safer landings on celestial bodies such as the Moon and Mars [1]. The second iteration of the DLC is known as the Engineering Test Unit (ETU) and one of its desired functionalities is the ability to preserve the fidelity of the system’s boot file through the use of memory scrubbing [2]. The ETU has two primary boards, one for housing a Multi-Processor System on a Chip (MPSoC) and the other for housing a Xilinx Kintex Ultrascale FPGA*. To emulate a memory scrubbing function implemented on the ETU’s FPGA board, the design and testing of a software application was performed on a Xilinx KCU105 FPGA evaluation board. The memory scrubbing application had to meet certain key criteria such as (1) properly utilize with the flash memory’s Serial Peripheral Interface (SPI) to read, write, and erase flash memory properly, (2) be able to detect arbitrarily large or small amounts of bit-errors, (3) be able to correct all detected errors, and (4) perform memory scrubbing indefinitely and autonomously. A prototype implementation was constructed and tested, demonstrating successful detection and correction of bit errors in multiple configurations. In the form of burst errors or singular bit flips, and in amounts of errors ranging from one to fifteen (per 256 Bytes), the application was successful in preserving memory fidelity.

Flash memory↗

NOR Flash Memory Scrubbing Application for Boot File Preservation of NASA’s Descent and Landing Computer (DLC)

Progress on NASA’s Safe and Precise Landing Integrated Capabilities Evolution (SPLICE)project continues, specifically with this development of the Descent and Landing Computer(DLC). One of the DLC’s primary contributions as a SPLICE technology is its implementationof algorithms and operation of sensors to autonomously guide a spacecraft in performing moreprecise and safer landings on celestial bodies such as the Moon and Mars. The second iterationof the DLC is known as the Engineering Test Unit (ETU) and one of its desired functionalitiesis the ability to preserve the fidelity of the system’s boot file through the use of memoryscrubbing. The ETU has two primary boards, one for housing a Multi-Processor System ona Chip (MPSoC) and the other for housing a Xilinx Kintex Ultrascale FPGA3. To emulate amemory scrubbing function implemented on the ETU’s FPGA board, the design and testingof a software application was performed on a Xilinx KCU105 FPGA evaluation board. Thememory scrubbing application had to meet certain key criteria such as (1) properly utilize withthe flash memory’s Serial Peripheral Interface (SPI) to read, write, and erase flash memoryproperly, (2) be able to detect arbitrarily large or small amounts of bit-errors, (3) be able tocorrect all detected errors, and (4) perform memory scrubbing indefinitely and autonomously.A prototype implementation was constructed and tested, demonstrating successful detectionand correction of bit errors in multiple configurations. In the form of burst errors or singularbit flips, and in amounts of errors ranging from one to fifteen (per 256 Bytes), the applicationwas successful in preserving memory fidelity.

Radiation tolerant↗

Lidar-Based Safe Site Relative Navigation

Established Safe and Precise Landing–Integrated Capability Evolution (SPLICE) project precision landing requirements necessitate a navigation filter architecture and underlying models developed specifically with these needs in mind. To date, test flights to characterize SPLICE guidance and navigation (GN) system performance have not provided a means to divert from the a priori selected landing site (LS) due to hazardous conditions. With the inclusion of a new sensor type, the Hazard Detection Lidar (HDL) coupled with safe landing site selection algorithms, GN can divert from the originally planned trajectory and navigate relative to the new targeted landing site. This discussion covers the navigation filter developments necessary to perform this estimation and process the resulting HDL measurements to meet project safe landing goals.

Navigation↗

Precision Landing Performance and Technology Assessments of a Human-Scale Lunar Lander Using a Generalized Simulation Framework

NASA has established goals of returning humans to Moon with an initial landing by 2024 and a subsequent sustained presence by 2028, which will require technological advances in spacecraft navigation to enable precision landing. The ability to assess the navigation performance of these new and existing technologies is critical to identifying areas of risk reduction and investment. To that end, the Safe and Precise Landing Integrated Capabilities Evolution (SPLICE) project has demonstrated that a detailed six degree-of-freedom integrated performance simulation framework can provide information on and assessment of expected navigation performance. This framework incorporates engineering models of the on-board spacecraft guidance, navigation, and control systems at varying levels of fidelity. Recent advances in the development of this integrated performance simulation permit running these systems “in-the-loop,” rather than assuming perfect knowledge of the spacecraft states. This development, coupled with fast simulation time and modularization of the various system models, enables a wide variety of system trades to be assessed at once. This paper presents a summary of the advances in the SPLICE simulation framework, updates to the spacecraft navigation models, and an application of the framework to characterize the precision landing performance of a human-scale lunar lander. A series of trade studies examining effects of ground state update qualities shows that given all other assumptions, sufficiently accurate Deep Space Network (DSN) measurements can enable safe and precise human-scale Lunar landings.

"D'Souza, Sarah", 'Pensado, Alegandro R.↗

Lidar-Based Safe Site Relative Navigation

There has been a renewed focus in exploration of the lunar surface and maximizing scientific potential of such missions is made possible in part by minimizing the time required to set up operations; that is, reducing transit time on the surface by increasing the landing precision with respect to the intended target. Established SPLICE project precision landing requirements necessitate a navigation filter architecture and underlying models developed specifically with these needs in mind. To date, test flights to characterize SPLICE GNC system performance have not provided a means to divert from the a priori selected landing site due to hazardous conditions. With the inclusion of a new sensor, the HDL, coupled with safe landing site selection algorithms, GNC can divert from the originally planned trajectory and navigate relative to the new targeted landing site. This work presents a novel hazard relative measurement model and covariance transformation methodology that enable the navigation system to inform a safe-site relative guidance profile to meet project precision landing goals.

Navigation↗

Space Technology Mission Directorate Game Changing Development Program

SPLICE project annual program review presentation. SPLICE Precision Landing and Hazard Avoidance (PL&HA) technologies will improve landing precision by an order of magnitude and enable high-resolution real-time 3-D mapping for hazard detection and avoidance during descent.

Guidance Navigation and Control↗

An integrated multi-omics approach identifies the landscape of interferon-α-mediated responses of human pancreatic beta cells

Proinflammatory cytokines are important mediators of pancreatic beta cell dysfunction and demise in the early stages of type 1 diabetes (T1D). Interferon-a (IFNa), a type I interferon member, is expressed in the islets of T1D individuals and it is expression and signaling is regulated by both genetic (T1D risk variants) and environmental factors (viral infections) associated to T1D. We presently characterized human beta cells responses to IFNa by combining ATAC-seq, RNA-seq and proteomics assays. The initial beta cell response to IFNa was characterized by major chromatin remodeling, followed by marked changes in transcriptional and translational regulation. IFNa-induced changes in alternative splicing (AS) and first exon usage increased the diversity of transcripts expressed by beta cells. This, combined with changes observed on protein modification/degradation, ER stress and MHC class I, may significantly expand the peptide repertoire presented by beta cells to the immune system. On the other hand, beta cells up-regulated checkpoint proteins, such as PDL1 and HLA-E, that may protect them against the autoimmune assault. Data mining of the present multi-omics analysis led to the identification of two compound classes that revert IFNa effects on human beta cells and may be translated to clinical trials.

60 APPLIED LIFE SCIENCES↗

GRIK phosphorylates and activates KIN10 which also promotes its degradation

The sensor kinase Sucrose Non-fermenting-1-Related Kinase 1 (SnRK1) plays a central role in energy and metabolic homeostasis. KIN10 is a major catalytic (a) kinase subunit of SnRK1 regulated by transcription, posttranslational modification, targeted protein degradation, and its subcellular localization. Geminivirus Rep Interacting Kinase 1 and 2 (GRIK1 and 2) are immediate upstream kinases of KIN10. In the transient protein expression assays carried out in Nicotiana benthamiana (N. benthamiana) leaves, GRIK1 not only phosphorylates KIN10 but also simultaneously initiates its degradation. Posttranslational GRIK-mediated KIN10 degradation is dependent on both GRIK kinase activity and phosphorylation of the KIN10 T-loop. KIN10 proteins are significantly enriched in the grik1-1 grik2-1 double mutant, consistent with the transient assays in N. benthamiana. Interestingly. Among the enriched KIN10 proteins from grik1-1 grik2-1, is a longer isoform, putatively derived by alternative splicing which is barely detectable in wild-type plants. The reduced stability of KIN10 upon phosphorylation and activation by GRIK represents a mechanism that enables the KIN10 activity to be rapidly reduced when the levels of intracellular sugar/energy are restored to their set point, representing an important homeostatic control that prevents a metabolic overreaction to low sugar conditions. Since GRIKs are activating kinases of KIN10, KIN10s in the grik1 grik2 double null mutant background remain un-phosphorylated, with only their basal level of activity, are more stable, and therefore increase in abundance, which also explains the longer isoform KIN10L which is a minor isoform in wild type is clearly detected in the grik1 grik2 double mutant.

59 BASIC BIOLOGICAL SCIENCES↗

Reduction of Chemokine CXCL9 Expression by Omega-3 Fatty Acids via ADP-Ribosylhydrolase ARH3 in MIN6 Insulin-Producing Cells

Type 1 diabetes (T1D) results from the autoimmune destruction of the insulin-producing β cells of the pancreas. Omega-3 fatty acids protect β cells and reduce the incidence of T1D, but the mechanism is poorly understood. We have shown that omega-3 fatty acids reduce pro-inflammatory cytokine-mediated β-cell apoptosis by upregulating the expression of the ADP-ribosylhydrolase ARH3. Here, we further investigate the β-cell protection mechanism of ARH3 by performing siRNA analysis of its gene Adprhl2 in MIN6 insulin-producing cells, subsequent treatment with a cocktail of the pro-inflammatory cytokines IL-1β + IFN-γ + TNF-α, followed by proteomics analysis. ARH3 regulated proteins from several pathways related to the nucleus (splicing, RNA surveillance, and nucleocytoplasmic transport), mitochondria (metabolic pathways), and endoplasmic reticulum (protein folding). ARH3 also regulated the levels of proteins related to antigen processing and presentation, and the chemokine-signaling pathway. We further studied the role of ARH3 in regulating the chemokine CXCL9. We found that ARH3 reduces the cytokine-induced expression of CXCL9, which is dependent on omega-3 fatty acids. In conclusion, we demonstrate that omega-3 fatty acids regulate CXCL9 expression via ARH3, which may have a role in protecting β cells from immune attack thereby preventing T1D development.

59 BASIC BIOLOGICAL SCIENCES↗

Image Provenance Analysis

The literature of multimedia forensics is mainly dedicated to the analysis of single assets (such as sole image or video files), aiming at individually assessing their authenticity. Different from this, image provenance analysis is devoted to the joint examination of multiple assets, intending to ascertain their history of edits, by evaluating pairwise relationships. Each relationship, thus, expresses the probability of one asset giving rise to the other, through either global or local operations, such as data compression, resizing, color-space modifications, content blurring, and content splicing. The principled combination of these relationships unveils the provenance of the assets, also constituting an important forensic tool for authenticity verification. This chapter introduces the problem of provenance analysis, discussing its importance and delving into the state-of-the-art techniques to solve it.

Moreira, Daniel↗