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DOE OSTI2025

Revisiting the Effects of Seed Liquid Water Content on Isoprene Secondary Organic Aerosol Growth

Aerosol liquid water content (LWC) has been shown to enhance partitioning of water-soluble oxygenated organic vapors into the aerosol particles and facilitate aqueous chemistry, impact SOA formation, composition, and physicochemical properties. Here, this study examines the effect of aerosol liquid water content (LWC) on isoprene SOA formation using effloresced and deliquesced ammonium sulfate ((NH 4 ) 2 SO 4 ) and sodium chloride (NaCl) seed particles in an environmental chamber with photo-oxidation extending over hours under moderate relative humidity (50%-60%). Our findings indicate that isoprene SOA mass yield is not significantly influenced (p value >> 0.05) by the phase state or the chemical identity of the seed particles within a 3–4-hour reaction time scale. Detailed molecular analysis using advanced high resolution mass spectrometry indicates that while both effloresced and deliquesced seed types produced similar molecular formula distributions, deliquesced (NH 4 ) 2 SO 4 slightly favored the production of less volatile compounds. Depth-profiling analysis by single particle mass spectrometry confirmed that isoprene SOA predominantly located on the surfaces of particles with core-shell morphologies, with partial dissolution of the effloresced inorganic salt observed even below the deliquescence relative humidity (DRH) of salt. This finding suggests that assuming dry salt core particles do not interact with SOA coatings, based on salt deliquescence points, may overlook important aqueous chemistry. These results emphasize the critical role of reaction time scale and mixing state on SOA formation and call for further research to understand LWC effects on SOA from different precursors.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

DOE OSTI2025

Targeted seed EMS mutagenesis reveals a basic helix–loop–helix transcription factor underlying male sterility in sorghum

Abstract Forward genetic screens of mutant populations are fundamental for functional genomics studies. However, isolating independent mutant alleles to molecularly identify causal genes is challenging in species recalcitrant to genetic manipulation. Here, we demonstrate that classic seed ethyl methanesulfonate (EMS) mutagenesis coupled with genome sequencing can overcome this limitation in sorghum. We used this method to generate new mutant alleles of sorghum MALE STERILE 8 (MS8) and identified the causal locus for the ms8 phenotype as Sobic.004G270900, which encodes the sorghum ortholog of maize bhlh122, a basic helix–loop–helix (bHLH) transcription factor required for male fertility in maize. Bulked segregant analysis mapped ms8-1 to a region on chromosome 4 containing Sobic.004G270900. Seeds from heterozygous MS8/ms8-1 plants were mutagenized and screened for chimeric inflorescences containing sectors with white, sterile anthers resembling the ms8-1 homozygous phenotype. DNA sequencing of sterile and fertile sectors from a single chimeric inflorescence revealed two mutations in Sobic.004G270900 within the sterile sector, but not the fertile sector. Isolation of this loss-of-function allele (ms8-2) established Sobic.004G270900 as the causative locus for male sterility in the ms8 mutant. We generated additional alleles of MS8 in a different genetic background using CRISPR/Cas9-based gene editing, where deletions in Sobic.004G270900 also resulted in male sterility. Our work identified a gene underlying male sterility in sorghum and provides a novel and straightforward genetic tool for researchers who lack access to advanced transformation facilities to validate gene candidates. Unlike gene editing, no prior knowledge of candidate genes is required for targeted seed EMS mutagenesis to aid identification of causal loci.

Genetics & Heredity↗

DOE OSTI2026

Massive black holes seeded by dark matter: Implications for little red dots and gravitational wave signatures

Observations of supermassive black holes (SMBHs) at high redshifts challenge standard seeding scenarios. We examine a dissipative self-interacting dark matter (dSIDM) model in which gravothermal collapse leads to the formation of massive BH seeds ab initio. We utilize a semianalytical framework to predict properties of the dSIDM-seeded SMBH population. Billion solar mass quasars are reproduced along with low-mass faint active galactic nuclei (known as little red dots) with SMBH-to-galaxy stellar mass ratios consistent with recent James Webb Space Telescope observations. To match the abundance of the observed bright quasars, a subpercent-level duty cycle is suggested, implying a large population of dormant SMBHs. The gravitational wave (GW) signals from mergers of these massive SMBHs can be detected by LISA while remaining within the NANOGrav constraints on the GW background. These results provide testable signatures of DM-driven SMBH formation, offering a pathway to probe hidden-sector physics through SMBH and GW observables.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

DOE OSTI2026

Multiplet lines in seeded stimulated Mn 𝐾⁢𝛼 1 x-ray emission

We report the successful resolution of the multiplet structure of 𝐾⁢𝛼 1 x-ray emission in manganese (Mn) complexes through seeded stimulated x-ray emission spectroscopy (seeded S-XES). Using a femtosecond pump pulse above the Mn 𝐾 edge to generate simultaneous 1⁢𝑠 core holes, and a second-color tunable seed pulse to initiate the stimulated emission process, we were able to enhance individual lines within the 𝐾⁢𝛼 1 emission. This approach allows to resolve the fine multiplet features that are obscured by lifetime broadening in conventional (spontaneous) Mn 𝐾⁢𝛼 XES. The work builds on our previous observation that S-XES from Mn(II) and Mn(VII) complexes pumped at high intensities can exhibit stimulated emission without sacrificing the chemical sensitivity to oxidation states. Furthermore, this technique opens the door to controlled high-resolution electronic structure spectroscopy in transition-metal complexes beyond the core-hole lifetime, with potential applications in catalysis, inorganic chemistry, and materials science.

Chemical Physics & Physical Chemistry↗

DOE OSTI2025

Genomic Analysis of the Natural Variation of Fatty Acid Composition in Seed Oils of Camelina sativa

Camelina sativa is an oilseed crop that has shown strong promise as a biofuel feedstock. The profile of fatty acids greatly influences the oil quality; however, genetic mechanisms that determine the natural variation of fatty acid composition in camelina are not fully understood. A genome wide association study (GWAS) was performed to uncover genetic loci that may contribute to the contents of major fatty acids such as oleic and linolenic acids in camelina seed. Two approaches were taken to improve the GWAS efficiency. First, growing a diversity panel of 212 accessions in four locations and two nitrogen fertilization conditions revealed great variation in fatty acid contents in seeds. Second, using an improved reference genome, abundant markers, including 203,320 single nucleotide polymorphisms (SNPs) and 99,067 insertions/deletions (indels), were developed, which refined the population structure of the diversity panel. GWAS resulted in 118 genetic markers across 31 trait/treatment conditions. Closely linked markers were determined based on linkage decay and by comparing secondarily associated markers when highly associated ones were removed. Candidate genes were examined by comparing the pangenomes of 12 high-quality reference genomes. This study provides new resources to understand seed lipid metabolism and improve camelina oils through molecular breeding.

Life Sciences & Biomedicine - Other Topics↗

DOE OSTI2026

Occurrence of seeding multi-layer clouds in the Arctic from ground-based observations

Studies of Arctic clouds often focus on low-level single-layer clouds (SLCs). Here, we use combined observations of soundings and cloud radar during the MOSAiC, ACSE, and AO2018 research cruises as well as from long-term observations at Ny-Ålesund, Svalbard and Utqiagvik, Alaska to investigate the occurrence of SLCs and multi-layer clouds (MLCs) in the Arctic and to assess the rate of ice-crystal seeding in cold MLCs. MOSAiC observations show cloudy conditions in between 70 % and 90 % of sounding-radar cases. SLCs show occurrence rates of 30 % to 40 % with the highest value of 45 % during October. Cold MLCs are most abundant from November to June (40 % to 55 % of cases). Seeding occurs in about half to two thirds of the identified cold MLCs during MOSAiC for which the sub-saturated layer extends between 100 and 1000 m. The seeding rate increases by as much as 20 percentage points as the assumed size of the falling ice crystals is increased from 100 to 400 µm. The observations reveal a stable rate of cloud-free conditions of around 20 % over the covered latitude range. Cloud occurrence during MOSAiC and at Ny-Ålesund in July, when the geographical distance between observations was minimal, shows reasonable agreement. Comparisons of MOSAiC and other research cruises to the central Arctic also indicate consistent occurrence rates of different cloud types despite the likely effect of year-to-year variability. The comparison of data from ship campaigns and land sites suggests that the latter are not necessarily a good indicator of cloud occurrence in the high Arctic.

Achtert, Peggy [Leipzig Univ. (Germany); Meteorolo↗

NASA NTRS1971

Opacity of tungsten-seeded hydrogen to 2500 K and 115 atmospheres.

Experimental investigation and measurement of the radiant heat attenuation of an aerosol which may serve as a gas core nuclear-rocket propellant. The experiment uses a tungsten-hydrogen aerosol heated to temperatures as high as 2500 K under pressures up to 115 atmospheres. The hydrogen aerosol is produced by dispersion of submicron-sized particles of tungsten in hydrogen gas. A narrow beam of broad spectrum (visible and ultraviolet) light is passed through it with the attenuation being measured as a function of wavelength. Other aerosol characteristics examined include the nature and extent of chemical reactions between the seed material and the hydrogen and the degree of dispersion of the seed material obtained before and after heating. Chemical equilibrium calculations and vapor pressure data for the refractory metals indicate that tungsten is a prime candidate for the seed material in the gas core nuclear rocket.

Williams, J. R.↗

NASA NTRS1977

Estimates of Optimal Operating Conditions for Hydrogen-Oxygen Cesium-Seeded Magnetohydrodynamic Power Generator

The value of percent seed, oxygen to fuel ratio, combustion pressure, Mach number, and magnetic field strength which maximize either the electrical conductivity or power density at the entrance of an MHD power generator was obtained. The working fluid is the combustion product of H2 and O2 seeded with CsOH. The ideal theoretical segmented Faraday generator along with an empirical form found from correlating the data of many experimenters working with generators of different sizes, electrode configurations, and working fluids, are investigated. The conductivity and power densities optimize at a seed fraction of 3.5 mole percent and an oxygen to hydrogen weight ratio of 7.5. The optimum values of combustion pressure and Mach number depend on the operating magnetic field strength.

Smith, J. M.↗

NASA NTRS1976

Wind tunnel flow seeding for laser velocimetry applications

Flow-seeding requirements for wind tunnel studies of subsonic and transonic flow fields are discussed with reference to laser velocimeter applications. Design of an aerosol generator with an output flow rate of 60 scfm is presented. The generated aerosol is fairly monodisperse; tracer particle diameter can be varied from 0.2 to 0.5 micron; and seeding density can be varied from 100 to 10,000 particles/cu sec. Experimental data on the use of this aerosol generator with various aerosol injection mechanisms indicate that the method is suitable for wind tunnel flow seeding.

Mazumder, M. K.↗

NASA NTRS1978

Germination of pine seed in weightlessness (investigation in Kosmos 782)

An investigation was made of the orientation of aboveground and underground organs of pine plants grown from seed in weightlessness. Orientation was found to be caused by the position of the seeds relative to the substrate surface. Normal growth was manifest only for the plants grown from seed oriented with embryo toward the substrate. Differences were noted between experiment and control as to the quantitative content of nucleoli in the meristematic cells of the rootlets and the shape of cells in the cotyledonous leaflets. No complete agreement was found between data obtained in weightlessness and when gravity was compensated (clinostat treatment with horizontal rotation).

Platonova, R. N.↗

NASA NTRS1979

Growth of silicon carbide crystals on a seed while pulling silicon crystals from a melt

A saturated solution of silicon and an element such as carbon having a segregation coefficient less than unity is formed by placing a solid piece of carbon in a body of molten silicon having a temperature differential decreasing toward the surface. A silicon carbide seed crystal is disposed on a holder beneath the surface of the molten silicon. As a rod or ribbon of silicon is slowly pulled from the melt, a supersaturated solution of carbon in silicon is formed in the vicinity of the seed crystal. Excess carbon is emitted from the solution in the form of silicon carbide which crystallizes on the seed crystal held in the cool region of the melt.

Ciszek, T. F.↗

NASA NTRS1982

Temperature-Controlled Support for a Seed Crystal

A rodlike structure called a sting has been proposed for supporting a seed crystal at center of a body of saturated fluid and for controlling temperature/time profile of seed for experiments on crystal growth. Seed crystal is cooled or heated by thermoelectric modules while surrounding sheath remains at solution temperature. Heat is withdrawn to cooling fins by heat pipe, which replaces solid copper rod in a previous design.

Reeve, J. L.↗

NASA NTRS1982

Segregation during directional melting and its implications on seeded crystal growth: A theoretical analysis

Directional melting of binary systems, as encountered during seeding in melt growth, is analyzed for concurrent compositional changes at the crystal-melt interface. It is shown that steady state conditions cannot normally be reached during seeding and that the growth interface temperature at the initial stages of seeded growth is a function of backmelt conditions. The theoretical treatment is numerically applied to Hg1-xCdXTe and Ga-doped Ge.

Bourret, E. D.↗

NASA NTRS1984

Experimental and theoretical deposition rates from salt-seeded combustion gases of a Mach 0.3 burner rig

Deposition rates on platinum-rhodium cylindrical collectors rotating in the cross streams of the combustion gases of a salt-seeded Mach 0.3 burner rig were determined. The collectors were internally air cooled so that their surface temperatures could be widely varied while they were exposed to constant combustion gas temperatures. The deposition rates were compared with those predicted by the chemically frozen boundary layer (CFBL) computer program, which is based on multicomponent vapor transport through the boundary layer. Excellent agreement was obtained between theory and experiment for the NaCl-seeded case, but the agreement lessened as the seed was changed to synthetic sea salt, NaNO3, and K2SO4, respectively, and was particularly poor in the case of Na2SO4. However, when inertial impaction was assumed to be the deposition mechanism for the Na2SO4 case, the predicted rates agreed well with the experimental rates. The former were calculated from a mean particle diameter that was derived from the measured intial droplet size distribution of the solution spray. Critical experiments showed that liquid phase deposits were blown off the smooth surface of the platinum-rhodium collectors by the aerodynamic shear forces of the high-velocity combustion gases but that rough or porous surfaces retained their liquid deposits.

Santoro, G. J.↗

NASA NTRS1984

Pre-existing seed particles and the onset of condensation in cryogenic wind tunnels

The condensation research at NASA Langley Research Center has used a variety of experimental approaches to gather information on seed particles that can act as sites for condensation growth. Total pressure measurements have suggested that condensation growth is caused by impurities in the flow and not by unevaporated liquid nitrogen (LN2) droplets resulting from the LN2 injected to cool the 0.3-Meter Transonic Cryogenic Tunnel. A separate test with an optical droplet sizing probe, which was designed to detect droplets in the 2- to 300-micron range, confirmed the conclusions from the total pressure measurements and also discovered what appears to be solidified oil droplets having diameters of about 3 microns. These oil droplets appear to be the dominant source of seed particles above 2 microns. However, computer simulations of static pressure test data suggest that the measured condensation effects are the result of more numerous, smaller seeds with number densities on the order of 10 to the 12th per kilogram of the gas and diameters on the order of 0.5 microns.

Hall, R. M.↗

NASA NTRS1985

Seeding materials: Health and safety considerations

The choice of a proper seeding material for laser velocimeters must include health and safety considerations. Failure to do so can lead to catastrophic results. All materials are toxic, and laser velocimeter seeding materials are no exception. Toxicity may be considered an inherent property of a given material. The manifestation of that property or the physiological response to the material is dependent on dose and exposure conditions. An approximate physiological classification of toxicity is given in tablular form. Toxicity in some situations is not necessarily the most restrictive factor in selection of materials. It is also very important to consider how the material is used so that actual exposure to the material in a damaging form can result. For example, nickel and cadmium are both extremely toxic as systemic poisons and in the case of nickel as a carcinogen. Seeding materials are dispersed in air under conditions that favor personnel exposure. Dispersal equipment is frequently if not normally manned, and personnel are often required to make frequent adjustments to assure proper operations.

Brown, R. D.↗

NASA NTRS1988

Performance of laser Doppler velocimeter with polydisperse seed particles in high speed flows

The flowfield behind an oblique shock wave, where the LDV measured velocities are seed particle size dependent, was used to investigate the effects of LDV system parameters on the range of detectable polydisperse seed particles. The parameters included frequency shifting, laser power, scattered signal amplification level, and number of required fringe crossings. The results showed that with polydisperse seed particles ranging from 0.1 to 4.0 microns available in the flow, the average diameter of the detected particles could change from 0.2 to 3.0 microns by changing different LDV system parameters. The effects of this shift in the range of detectable particles on the frequency response of LDV was discussed.

Samimy, M.↗

NASA NTRS1989

Performance of laser Doppler velocimeter with polydisperse seed particles in high-speed flows

The flowfield behind an oblique shock wave, where the LDV measured velocities are seed-particle-size dependent, was used to investigate the effects of LDV system parameters on the range of detectable polydisperse seed particles. The parameters included frequency shifting, laser power, scattered signal amplification level, and number of required fringe crossings. The results showed that with polydisperse seed particles ranging from 0.1 to 4.0 microns available in the flow, the average diameter of the detected particles could change from 0.2 to 3.0 microns by changing different LDV system parameters. The effects of this shift in the range of detectable particles on the frequency response of LDV are discussed.

Samimy, M.↗